BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cnidium21_contig00007658
         (3062 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAB46363.1| DNA topoisomerase I [Daucus carota]                  1390   0.0  
dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]              1143   0.0  
dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]      1133   0.0  
dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]                1127   0.0  
dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]               1118   0.0  

>emb|CAB46363.1| DNA topoisomerase I [Daucus carota]
          Length = 909

 Score = 1390 bits (3597), Expect = 0.0
 Identities = 722/915 (78%), Positives = 770/915 (84%), Gaps = 17/915 (1%)
 Frame = -3

Query: 2994 MDNGSDSEDDRPLVFKRSTPSVKQNQVKPDVKKTSMQKPDSKSGRQMSDVRSPNGNGHDS 2815
            M++GSDSEDD+PL+FKRS  SVKQNQ KP  +K    +   + GR +SDVRS NG  HDS
Sbjct: 1    MEHGSDSEDDKPLIFKRSNTSVKQNQAKPRCQKIISAEAVWQVGRLISDVRSTNG--HDS 58

Query: 2814 NIQKGKTVPSTKGXXXXXXXXXXXXXXXXXXXXXXXXPVASTKASTSGADKSRQNIQQNK 2635
            N+QKGKTVPSTKG                        PVASTKASTSGAD+SRQN QQN+
Sbjct: 59   NVQKGKTVPSTKGSPVVSPLASPKASVSSSKASQVKPPVASTKASTSGADQSRQNSQQNR 118

Query: 2634 IATVKADTPSGKPKDEPDNGSEDSDDDKPLNSRLPVGGSKGSITELNKRPGSSGPASLAK 2455
             A VKA+ PSG PK+EPD+GSEDSDDDKPLNSRLPVGGSKGSI+E+N+RP SSGPA+  K
Sbjct: 119  TAIVKAERPSGIPKEEPDSGSEDSDDDKPLNSRLPVGGSKGSISEVNRRPSSSGPAAFEK 178

Query: 2454 PDVGVKEEDSEDDIPLSHKFKQKSNAGESRNAYDSDDIKPFASKHQQNGTSARDNVKNPS 2275
             D+G+KEEDSEDDIPLSHKFKQKSNAGES NAYDSD+ KPFASK +QNGT+ +DNVKNPS
Sbjct: 179  -DLGIKEEDSEDDIPLSHKFKQKSNAGESSNAYDSDEGKPFASKPRQNGTNVKDNVKNPS 237

Query: 2274 TALNKRPSAEVKCSGQSPVKKAKHTATSTPVNGKKVSVKAEPKKEXXXXXXDNATISQRV 2095
            TA+NKRPSAEVKCSGQSPVKKA        V  +K++VKAEPKKE      DNATISQRV
Sbjct: 238  TAINKRPSAEVKCSGQSPVKKATVQLHLHQV--RKLTVKAEPKKEADDSDDDNATISQRV 295

Query: 2094 KKPVTPIXXXXXXXXXXXXXXXXXXXXXXXXXKITKTTQFSKSLKVPPGSGEGQKWITLV 1915
            KKP TPI                         KITK+TQFSKSLKVPPGSGEGQKW TLV
Sbjct: 296  KKPATPISKASDKKKSAVSVSSSLNKVNKNSKKITKSTQFSKSLKVPPGSGEGQKWTTLV 355

Query: 1914 HNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMIAVMQD-TEYMTKPVFRENVMNDW 1738
            HNGVIFPPPYKPHGVKMLYK  PVDLT  QEEV  MIAVMQ  TEY+TKPVFRENVMNDW
Sbjct: 356  HNGVIFPPPYKPHGVKMLYKESPVDLTLNQEEVH-MIAVMQILTEYLTKPVFRENVMNDW 414

Query: 1737 KKILGRNHIIKNLDDCDFTPIYEWHQSEKEKKKQMSAEEKKALREEKMKQEEKYMWAIVD 1558
            K ILGRNHIIKNLDDCDFTPIYEW + +KEKKKQMSAEEKKA++EEK+KQEEKYMWA+VD
Sbjct: 415  KIILGRNHIIKNLDDCDFTPIYEWFRLKKEKKKQMSAEEKKAVKEEKLKQEEKYMWAVVD 474

Query: 1557 GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIYPSDITINIGGDAPIPECPIPGESWKE 1378
            GVKE VGNFRVEPPGLFRGR   PKMGKLKKRI PSDITINIG DAPIPECPIPGESWKE
Sbjct: 475  GVKENVGNFRVEPPGLFRGRESIPKMGKLKKRISPSDITINIGKDAPIPECPIPGESWKE 534

Query: 1377 VRHDNTVTWLAYWLDPINGKEFKYVFLAASSSLKGQSDKEKYEKARKLKGYIAGIRTAYT 1198
            VRHDNTVTWLAYW DPINGKEFKYVFLAASSSLKGQSDKEKYEKARKLKGYI GIRTAYT
Sbjct: 535  VRHDNTVTWLAYWNDPINGKEFKYVFLAASSSLKGQSDKEKYEKARKLKGYIEGIRTAYT 594

Query: 1197 KDFANKDVAKRQI----------------AVATYLIDKLALRAGNEKDDDEADTVGCCTL 1066
            KDF++KDV KRQI                AVATYLIDKLALRAGNEKDDDEADTVGCCTL
Sbjct: 595  KDFSSKDVTKRQIAVATYLIDKLALRAGNAVATYLIDKLALRAGNEKDDDEADTVGCCTL 654

Query: 1065 KVENVEPSPPNILKLDFLGKDSIRYQNEVEVEIPVFKAIQQFRSGKEGSDNLFDKLDTTK 886
            KVENVEP    +LKLDFL KDSIRYQ  VEVEIPVFKAIQQFRSGK+GSDNLFDKLDT K
Sbjct: 655  KVENVEPRNNPLLKLDFLPKDSIRYQKRVEVEIPVFKAIQQFRSGKKGSDNLFDKLDTCK 714

Query: 885  LNAHLKELMPGLSAKVFRTYNASITLDEMLNKETKGGDVSQKVAVYQHANKEVAIICNHQ 706
            LNAHLKELMPGLSAKVFRTYNASITLDEMLNKETKGGDVSQKVAVYQHANKEVAIICNHQ
Sbjct: 715  LNAHLKELMPGLSAKVFRTYNASITLDEMLNKETKGGDVSQKVAVYQHANKEVAIICNHQ 774

Query: 705  RSVSKSHSTQMLRLDEKINELKDLVEELKTDLSRAKKGKPPLKGSDGKIKRNINPEALEK 526
            RSVSKSHSTQM+RLDEKIN+LK+ +EELKTDLSRAKKGKPPLKGSDGK KRN+NPEA+EK
Sbjct: 775  RSVSKSHSTQMMRLDEKINKLKNFIEELKTDLSRAKKGKPPLKGSDGKTKRNMNPEAIEK 834

Query: 525  KIAQTDAKIEKMERDKEIKEDLKTVALGTSKINYLDPRITVAWCKRYEVPIEKMFNKSLL 346
            KIAQT+ KIEKMERDKE KEDLKTVALGTSKINYLDPRITVAWCKRYEVPIEKMFNKSLL
Sbjct: 835  KIAQTNVKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRYEVPIEKMFNKSLL 894

Query: 345  AKFAWAMDVEPTFRF 301
            AKFAWAMDVEP+FRF
Sbjct: 895  AKFAWAMDVEPSFRF 909


>dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]
          Length = 894

 Score = 1143 bits (2957), Expect = 0.0
 Identities = 596/895 (66%), Positives = 693/895 (77%), Gaps = 4/895 (0%)
 Frame = -3

Query: 2973 EDDRPLVFKRSTPSVKQNQVKPDVKKTSMQKPDSKSGRQMSDVRSPNGNGHDSNIQKGKT 2794
            EDD PLVFKRS  + K NQ+  D KK+S Q+ D +SGRQ+SDV  P+ NG  S+  KGKT
Sbjct: 17   EDDEPLVFKRSNATSKHNQLNSDTKKSS-QRYDGQSGRQVSDV--PSTNGQSSSASKGKT 73

Query: 2793 VPSTKGXXXXXXXXXXXXXXXXXXXXXXXXPVASTKASTSGADKSRQNIQQNKIATVKAD 2614
               +                          PVA+ K S+S  D+S+ + +QN I  VK +
Sbjct: 74   TKLSS-------MKSPIASPKASTSFAKASPVANPKVSSSSDDRSKHSSKQNTINVVKEE 126

Query: 2613 TPSGKPKDEPDNGSEDSDDDKPLNSRLPVGGSKGSITELNKRPGSSGPASLA--KPDVGV 2440
                 P  EP     DS+D+KPL++RL  G +KGS    NK   +S PASL   KP++  
Sbjct: 127  KELVNPAAEPYG---DSEDEKPLSARLFTGLTKGSSNNANKGLINSSPASLPVPKPEINR 183

Query: 2439 KEEDSEDDIPLSHKFKQKSNAGESR-NAYDSDDIKPFASKHQQNGTSARDNVKNPSTALN 2263
              +DS+D+IPLS KF+ K+NAG S   +Y+SDD K   S  QQNG+  R +    S  +N
Sbjct: 184  YSDDSDDEIPLSSKFRLKANAGTSTVKSYESDDNKTLVSNFQQNGSINRGS--KSSIKVN 241

Query: 2262 KRPSAEVKCSGQSPVKKAKHTATSTPVNGKKVSVKAEPKKEXXXXXXDNATISQRVKKPV 2083
            KRP  EVK S QS VKK K +  STPVN K+ S KAEPK +       +  ISQR+KK  
Sbjct: 242  KRPLGEVKSSVQSSVKKPKLSDASTPVNNKQASKKAEPKADDSDDD--HVPISQRMKKSA 299

Query: 2082 TP-IXXXXXXXXXXXXXXXXXXXXXXXXXKITKTTQFSKSLKVPPGSGEGQKWITLVHNG 1906
            T                            K+ K +++SKS KVPP SGEG+KW TLVHNG
Sbjct: 300  TSGNKSSTAKQKVTKVVSSSMKKASKKFKKVVKKSKYSKSSKVPPSSGEGKKWNTLVHNG 359

Query: 1905 VIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMIAVMQDTEYMTKPVFRENVMNDWKKIL 1726
            VIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATM AVM DT+YMTK  F+EN M+DW+KIL
Sbjct: 360  VIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKSKFKENFMDDWRKIL 419

Query: 1725 GRNHIIKNLDDCDFTPIYEWHQSEKEKKKQMSAEEKKALREEKMKQEEKYMWAIVDGVKE 1546
            G+NH+I+NL+DCDFTPIYEWHQ EKEKKK+M+AEEKK L+EE++KQEEKYMWAIV+GVKE
Sbjct: 420  GKNHVIQNLEDCDFTPIYEWHQREKEKKKKMTAEEKKVLKEERLKQEEKYMWAIVNGVKE 479

Query: 1545 KVGNFRVEPPGLFRGRGEHPKMGKLKKRIYPSDITINIGGDAPIPECPIPGESWKEVRHD 1366
            KVGNFRVEPP LFRGRGEHPKMGKLKK I PSDITINIG DAPIPECPIPGESWKE+RHD
Sbjct: 480  KVGNFRVEPPSLFRGRGEHPKMGKLKKCIRPSDITINIGKDAPIPECPIPGESWKEIRHD 539

Query: 1365 NTVTWLAYWLDPINGKEFKYVFLAASSSLKGQSDKEKYEKARKLKGYIAGIRTAYTKDFA 1186
            NTVTWLA+W DPI  +EFKYVFLAASSSLKGQSDKEKYEKAR LK +I GIR AYTKDFA
Sbjct: 540  NTVTWLAFWNDPIKPREFKYVFLAASSSLKGQSDKEKYEKARLLKDFIQGIRAAYTKDFA 599

Query: 1185 NKDVAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPSPPNILKLDFLGK 1006
            +KD+ KRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEP PP+ILK DFLGK
Sbjct: 600  SKDITKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPKPPSILKFDFLGK 659

Query: 1005 DSIRYQNEVEVEIPVFKAIQQFRSGKEGSDNLFDKLDTTKLNAHLKELMPGLSAKVFRTY 826
            DSIRYQNEVEVE+PV+KAIQQFR+GK G D+LFDKLDT+KLNAHLK LMPGL+AKVFRTY
Sbjct: 660  DSIRYQNEVEVELPVYKAIQQFRTGKRGGDDLFDKLDTSKLNAHLKGLMPGLTAKVFRTY 719

Query: 825  NASITLDEMLNKETKGGDVSQKVAVYQHANKEVAIICNHQRSVSKSHSTQMLRLDEKINE 646
            NASITLDEML++ETKGG+V++K+ VYQHANKEVAIICNHQR+VSKSH  QM RL+ KI+E
Sbjct: 720  NASITLDEMLSRETKGGNVAEKIVVYQHANKEVAIICNHQRTVSKSHPAQMTRLNGKIDE 779

Query: 645  LKDLVEELKTDLSRAKKGKPPLKGSDGKIKRNINPEALEKKIAQTDAKIEKMERDKEIKE 466
            LK +++ L+TDL+RAKKGKPPL+ +DGK KRN+ PEALE+KI QT+AKIEKMERDKE KE
Sbjct: 780  LKGILDGLQTDLARAKKGKPPLEDADGKPKRNLTPEALERKIGQTNAKIEKMERDKETKE 839

Query: 465  DLKTVALGTSKINYLDPRITVAWCKRYEVPIEKMFNKSLLAKFAWAMDVEPTFRF 301
             LKT+ALGTSKI+YLDPRITVAWCKR+EVPIEK+FNKSLLAKFAW+MDV+P+FRF
Sbjct: 840  GLKTIALGTSKISYLDPRITVAWCKRHEVPIEKVFNKSLLAKFAWSMDVDPSFRF 894


>dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]
          Length = 925

 Score = 1133 bits (2931), Expect = 0.0
 Identities = 600/919 (65%), Positives = 684/919 (74%), Gaps = 26/919 (2%)
 Frame = -3

Query: 2979 DSEDDR-PLVFKRSTPSVKQNQVKPDVKKTSMQKPDSKSGRQMSDVRSPNGNGHDSNIQK 2803
            D EDD  P++FKRS P+ KQNQ   + KK S+QK D +S    SDVR    NG  S+ QK
Sbjct: 13   DMEDDGGPVIFKRSKPAPKQNQANSEKKKLSLQKQDGQSAVHTSDVRP--ANGESSSAQK 70

Query: 2802 GKTVPSTKGXXXXXXXXXXXXXXXXXXXXXXXXPVASTKASTSGADKSRQNIQQNKIATV 2623
            G+  PS K                         P A++K S S    ++Q       A  
Sbjct: 71   GRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAKQQNHHKSSAAF 130

Query: 2622 KADTPSGKPKDEPDNGSEDSDDDKPLNSRLPVGGSKGSITELNKRPGSSGPASLAKPDVG 2443
                PS  P  + ++ SE SDD+KPL++R     SKG     NK  G S   S+ +P + 
Sbjct: 131  IEPKPS-VPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDS--CSIRRPVIE 187

Query: 2442 VKEEDSEDDIPLSHKFKQKSNAGESRN-AYDSDDIKPFASKHQQNGTSARDNVKNPSTAL 2266
             + EDSED+ PLS +F  KS+ GES + +YDSD+ KP A+K QQNG+++RD   N S +L
Sbjct: 188  -ESEDSEDEKPLSCRFTSKSSLGESTSKSYDSDEKKPLAAKVQQNGSASRDGPMNKSASL 246

Query: 2265 -NKRPSAEVKCSGQSPVKKAKHTATSTPVNGKKVSVKAEPKKEXXXXXXD---------- 2119
             NKRP  E K   QS VKK K ++  T ++ K+ +VK EPK E                 
Sbjct: 247  SNKRPPGEAKSLNQSSVKKPKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQRGKKSQS 306

Query: 2118 -----------NATISQRVKK-PVTPIXXXXXXXXXXXXXXXXXXXXXXXXXKITKTTQF 1975
                       N  ISQR+KK P +                           K+ K T +
Sbjct: 307  ATSKPKDDDDDNVPISQRIKKSPASVSKSSVPVKKASTVVSSSLKKVNKNSKKVIKKTAY 366

Query: 1974 SKSLKVPPGSGEGQKWITLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMIAVM 1795
            +KS KVPPGSGEGQKW TLVHNGVIFPPPYKPHGVKMLYK +P+ LTPEQEEVATM AVM
Sbjct: 367  TKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEVATMFAVM 426

Query: 1794 QDTEYMTKPVFRENVMNDWKKILGRNHIIKNLDDCDFTPIYEWHQSEKEKKKQMSAEEKK 1615
             DT+YM KP F+EN  +DWKKILG+NH+I+NL+DCDF PIYEWHQ EKEKKKQM+ EEKK
Sbjct: 427  LDTDYMNKPCFKENFFSDWKKILGKNHMIQNLEDCDFGPIYEWHQQEKEKKKQMTTEEKK 486

Query: 1614 ALREEKMKQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIYPSDITIN 1435
            AL+EEK++QEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI P DITIN
Sbjct: 487  ALKEEKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITIN 546

Query: 1434 IGGDAPIPECPIPGESWKEVRHDNTVTWLAYWLDPINGKEFKYVFLAASSSLKGQSDKEK 1255
            IG DAPIPECPIPGE WKEVR+ NTVTWLAYW DPIN KEFKYVFLAASS+LKGQSDKEK
Sbjct: 547  IGKDAPIPECPIPGERWKEVRNGNTVTWLAYWNDPINQKEFKYVFLAASSTLKGQSDKEK 606

Query: 1254 YEKARKLKGYIAGIRTAYTKDFAN-KDVAKRQIAVATYLIDKLALRAGNEKDDDEADTVG 1078
            YEKAR LK YI GIR AYTKDF N KD  K+QIAVATYLIDKLALRAGNEKDDDEADTVG
Sbjct: 607  YEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDDDEADTVG 666

Query: 1077 CCTLKVENVEPSPPNILKLDFLGKDSIRYQNEVEVEIPVFKAIQQFRSGKEGSDNLFDKL 898
            CCTLKVENVEP PPNILK DFLGKDSIRYQNEV+VE  VFKAIQQFRSGKEGS++LFD+L
Sbjct: 667  CCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGSEDLFDRL 726

Query: 897  DTTKLNAHLKELMPGLSAKVFRTYNASITLDEMLNKETKGGDVSQKVAVYQHANKEVAII 718
            DT+KLNAHLKELMPGL+AKVFRTYNASITLD+ML+KETKGG+V++ V VYQHANKEVAII
Sbjct: 727  DTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGEVAENVVVYQHANKEVAII 786

Query: 717  CNHQRSVSKSHSTQMLRLDEKINELKDLVEELKTDLSRAKKGKPPLKGSDGKIKRNINPE 538
            CNHQR+VSKSHS QM RL+EKI+ELK  +EELKTDLSRAKKGKPP KG+DGK KRN+NPE
Sbjct: 787  CNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPPTKGADGKAKRNLNPE 846

Query: 537  ALEKKIAQTDAKIEKMERDKEIKEDLKTVALGTSKINYLDPRITVAWCKRYEVPIEKMFN 358
            ALE+KI QT+AKIEKMERDKE KEDLKTVALGTSKINYLDPRITVAWCKR+EVPIEK+FN
Sbjct: 847  ALERKITQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFN 906

Query: 357  KSLLAKFAWAMDVEPTFRF 301
            KSLLAKFAW+MDV+P+FRF
Sbjct: 907  KSLLAKFAWSMDVDPSFRF 925


>dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]
          Length = 910

 Score = 1127 bits (2915), Expect = 0.0
 Identities = 598/911 (65%), Positives = 679/911 (74%), Gaps = 20/911 (2%)
 Frame = -3

Query: 2973 EDDRPLVFKRSTPSVKQNQVKPDVKKTSMQKPDSKSGRQMSDVRSPNGNGHDSNIQKGKT 2794
            EDD P+VFKR+  + KQ+Q   + +K S QK D +S RQ+ D R    NG  S+ QKG+ 
Sbjct: 17   EDDEPIVFKRNNSASKQSQTNSETRKVSSQKRDGQSVRQIYDSRP--ANGESSSGQKGRM 74

Query: 2793 VPSTKGXXXXXXXXXXXXXXXXXXXXXXXXPV--------------ASTKASTSGADKSR 2656
            VP +K                          V              A++K STSG+ K +
Sbjct: 75   VPPSKPSQVKSPLSSPPSKPSQVKSPLSSPKVSTSSAKKSPVSSLPANSKPSTSGSFKVK 134

Query: 2655 QNIQQNKIATVKADTPSGKPK--DEPDNGSEDSDDDKPLNSRLPVGGSKGSITELNKRPG 2482
                Q+K ATV  +  S   +      N SEDSDD KPL+ R     SKG+  +++K   
Sbjct: 135  P-FNQHKSATVIKEEKSSPLRLASAIINDSEDSDDGKPLSLRHSASSSKGNTNQVSKEG- 192

Query: 2481 SSGPASLAKPDVGVKEEDSEDDIPLSHKFKQKSNAGESRN-AYDSDDIKPFASKHQQNGT 2305
                   +KP+     EDS+D+ PLS +F  KS  GES + AYDSDD KP A K Q NG+
Sbjct: 193  -------SKPE----NEDSDDEKPLSSRFPLKSGVGESTSKAYDSDDDKPLAKKLQHNGS 241

Query: 2304 SARDNVKNP-STALNKRPSAEVKCSGQSPVKKAKHTATSTPVNGKKVSVKAEPKKEXXXX 2128
            + RD   N  S   NKRP  ++K S Q  VKK K ++ + P N K+ SVK EPK E    
Sbjct: 242  AMRDGQPNKYSNMSNKRPPGDIKSSNQLSVKKPKLSSVTAPTNKKQASVKPEPKAEDDDD 301

Query: 2127 XXDNATISQRVKKPVTPIXXXXXXXXXXXXXXXXXXXXXXXXXKITKTTQFSKSLKVPPG 1948
               +  ISQR+KK  T                           K+ K +++SKS KVPP 
Sbjct: 302  D--HLPISQRIKKSPTSESKSSSIKKTTKVVSSSVKKVNKKSKKVMKNSKYSKSSKVPPS 359

Query: 1947 SGEGQKWITLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMIAVMQDTEYMTKP 1768
            SGEG+KW  L HNGVIFPPPYKPHG+KMLYKGKPVDLTPEQEEVATM AVM DT+YM KP
Sbjct: 360  SGEGKKWNKLEHNGVIFPPPYKPHGIKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMNKP 419

Query: 1767 VFRENVMNDWKKILGRNHIIKNLDDCDFTPIYEWHQSEKEKKKQMSAEEKKALREEKMKQ 1588
             F+EN   DWKKILG+NH+I+NL++CDF+PIYEWHQSEKEKKKQM+ EEKKAL+EEK+KQ
Sbjct: 420  RFKENFFGDWKKILGKNHVIQNLENCDFSPIYEWHQSEKEKKKQMTTEEKKALKEEKLKQ 479

Query: 1587 EEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIYPSDITINIGGDAPIPE 1408
            EEKYMWA VDG KEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI P DITINIG DAPIPE
Sbjct: 480  EEKYMWAWVDGKKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPCDITINIGKDAPIPE 539

Query: 1407 CPIPGESWKEVRHDNTVTWLAYWLDPINGKEFKYVFLAASSSLKGQSDKEKYEKARKLKG 1228
            CP+PGE WKEVRHDNTVTWLA+W DPIN KEFKYVFLAASS+LKG SDKEKYEKAR LK 
Sbjct: 540  CPVPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSTLKGLSDKEKYEKARLLKD 599

Query: 1227 YIAGIRTAYTKDFANKDVAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE 1048
            YI GIR AYTKDFA+KD  K+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE
Sbjct: 600  YIQGIRAAYTKDFASKDPTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE 659

Query: 1047 PSPPNILKLDFLGKDSIRYQNEVEVEIPVFKAIQQFRSGKEGSDNLFDKLDTTKLNAHLK 868
            P PPNILK DFLGKDSIRYQNEVEVE  VFKAIQQFRSGKEGS +LFD LDT+KLNAHLK
Sbjct: 660  PVPPNILKFDFLGKDSIRYQNEVEVEAAVFKAIQQFRSGKEGSSDLFDLLDTSKLNAHLK 719

Query: 867  ELMPGLSAKVFRTYNASITLDEMLNKETKGGDVSQKVAVYQHANKEVAIICNHQRSVSKS 688
            ELMPGL+AKVFRTYNASITLD+ML++ETKGGDV++KV VYQHANKEVAIICNHQR+VSKS
Sbjct: 720  ELMPGLTAKVFRTYNASITLDDMLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKS 779

Query: 687  HSTQMLRLDEKINELKDLVEELKTDLSRAKKGKPPL--KGSDGKIKRNINPEALEKKIAQ 514
            HS QMLRL+EKI ELK +VEELK+DLSR KKGKPPL  K +DGK KRN+NPE+LEKKIAQ
Sbjct: 780  HSAQMLRLNEKIEELKAVVEELKSDLSRVKKGKPPLKSKNADGKPKRNLNPESLEKKIAQ 839

Query: 513  TDAKIEKMERDKEIKEDLKTVALGTSKINYLDPRITVAWCKRYEVPIEKMFNKSLLAKFA 334
            T+AKIEKMERDKE KEDLKTVALGTSKINYLDPRITVAWCKR+EVPIEK+FNKSLLAKF 
Sbjct: 840  TNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFT 899

Query: 333  WAMDVEPTFRF 301
            WAMDV+P+FRF
Sbjct: 900  WAMDVDPSFRF 910


>dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]
          Length = 927

 Score = 1118 bits (2893), Expect = 0.0
 Identities = 593/921 (64%), Positives = 681/921 (73%), Gaps = 28/921 (3%)
 Frame = -3

Query: 2979 DSEDDR-PLVFKRSTPSVKQNQVKPDVKKTSMQKPDSKSGRQMSDVRSPNGNGHDSNIQK 2803
            D EDD  P++FKRS P+ KQNQ   + KK S+QK   +S    SDVR    NG  S+ QK
Sbjct: 13   DMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVRP--ANGESSSAQK 70

Query: 2802 GKTVPSTKGXXXXXXXXXXXXXXXXXXXXXXXXPVASTKASTSGADKSRQNIQQNKIATV 2623
            G+  PS K                         P A++K S S    ++Q       A  
Sbjct: 71   GRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAKQQNHHKSSAAF 130

Query: 2622 KADTPSGKPKDEPDNGSEDSDDDKPLNSRLPVGGSKGSITELNKRPGSSGPASLAKPDVG 2443
                PS  P  + ++ SE SDD+KPL++R     SKG     NK  G S      +  V 
Sbjct: 131  IEPKPS-VPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDS---CSIRRRVI 186

Query: 2442 VKEEDSEDDIPLSHKFKQKSNAGESRN-AYDSDDIKPFASKHQQNGTSARDNVKNPSTAL 2266
             + EDSED+ PLS +F  KS+ GES + +YDSD+ KP A+K QQNG+ +RD   N S +L
Sbjct: 187  EESEDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPLAAKVQQNGSVSRDGPMNKSASL 246

Query: 2265 -NKRPSAEVKCSGQSPVKKAKHTATSTPVNGKKVSVKAEPK------------------- 2146
             NKRP  EVK   QS VKK K ++  T ++ K+ +VK EP+                   
Sbjct: 247  SNKRPPGEVKSLNQSSVKKPKLSSPITSISNKQAAVKPEPEPKAEDDDDNIPLSQRGKKS 306

Query: 2145 ----KEXXXXXXDNATISQRVKK-PVTPIXXXXXXXXXXXXXXXXXXXXXXXXXKITKTT 1981
                 +      DN  I+QR+KK P +                           K+ K T
Sbjct: 307  QAATSKPKDEDDDNVPIAQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKNSKKVIKKT 366

Query: 1980 QFSKSLKVPPGSGEGQKWITLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMIA 1801
             ++KS KVPPGSGEGQKW TLVHNGVIFPPPY PHGVKMLYK +P+ LTPEQEEVATM A
Sbjct: 367  AYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYNPHGVKMLYKRQPITLTPEQEEVATMFA 426

Query: 1800 VMQDTEYMTKPVFRENVMNDWKKILGRNHIIKNLDDCDFTPIYEWHQSEKEKKKQMSAEE 1621
            VM DT+YM KP F+EN  +DWKK+LG+NH I+NL+DCDF PIYEWHQ EKEKKKQM+ EE
Sbjct: 427  VMLDTDYMNKPRFKENFFSDWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKKKQMTTEE 486

Query: 1620 KKALREEKMKQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIYPSDIT 1441
            KKAL+E+K++QEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI P DIT
Sbjct: 487  KKALKEDKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDIT 546

Query: 1440 INIGGDAPIPECPIPGESWKEVRHDNTVTWLAYWLDPINGKEFKYVFLAASSSLKGQSDK 1261
            INIG DAPIPECPIPGE WKEVR+DNTVTWLA+W+DPIN KEFKYVFLAASS+LKGQSDK
Sbjct: 547  INIGKDAPIPECPIPGERWKEVRNDNTVTWLAFWIDPINQKEFKYVFLAASSTLKGQSDK 606

Query: 1260 EKYEKARKLKGYIAGIRTAYTKDFAN-KDVAKRQIAVATYLIDKLALRAGNEKDDDEADT 1084
            EKYEKAR LK YI GIR AYTKDF N KD  K+QIAVATYLIDKLALRAGNEKDDDEADT
Sbjct: 607  EKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDDDEADT 666

Query: 1083 VGCCTLKVENVEPSPPNILKLDFLGKDSIRYQNEVEVEIPVFKAIQQFRSGKEGSDNLFD 904
            VGCCTLKVENVEP PPNILK DFLGKDSIRYQNEV+VE  VFKAIQQFRSGKEGS++LFD
Sbjct: 667  VGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGSEDLFD 726

Query: 903  KLDTTKLNAHLKELMPGLSAKVFRTYNASITLDEMLNKETKGGDVSQKVAVYQHANKEVA 724
            +LDT+KLNAHLKELMPGL+AKVFRTYNASITLD+ML+KETKGG+V++KV VYQHANKEVA
Sbjct: 727  RLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGEVAEKVVVYQHANKEVA 786

Query: 723  IICNHQRSVSKSHSTQMLRLDEKINELKDLVEELKTDLSRAKKGKPPLKGSDGKIKRNIN 544
            IICNHQR+VSKSHS QM RL+EKI+ELK  +EELKTDLSR KKGKPP KG+DGK KRN+N
Sbjct: 787  IICNHQRTVSKSHSAQMSRLNEKIDELKTAMEELKTDLSRVKKGKPPSKGADGKPKRNLN 846

Query: 543  PEALEKKIAQTDAKIEKMERDKEIKEDLKTVALGTSKINYLDPRITVAWCKRYEVPIEKM 364
            PEALE+K+AQ +AKIEKMERDKE KEDLKTVALGTSKINYLDPRITVAWCKR+EVPIEK+
Sbjct: 847  PEALERKMAQINAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKI 906

Query: 363  FNKSLLAKFAWAMDVEPTFRF 301
            FNKSLLAKFAW+MDV+P+FRF
Sbjct: 907  FNKSLLAKFAWSMDVDPSFRF 927