BLASTX nr result
ID: Cinnamomum25_contig00034665
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cinnamomum25_contig00034665
(289 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica] 83 8e-14
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran... 80 4e-13
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;... 80 4e-13
gb|AJD20224.1| coclaurine N-methyltransferase [Sinopodophyllum h... 75 2e-11
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum] 73 6e-11
gb|AKH61489.1| N-methyltransferase [Expression vector pWCD2353] 69 2e-09
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf... 69 2e-09
gb|ADP76529.1| coclaurine N-methyltransferase [Aristolochia fimb... 63 7e-08
sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin... 63 9e-08
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi... 60 6e-07
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin... 60 6e-07
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro... 58 3e-06
>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
Length = 358
Score = 82.8 bits (203), Expect = 8e-14
Identities = 40/75 (53%), Positives = 59/75 (78%)
Frame = -2
Query: 225 MAVDSNSKPAKKASVVELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLL 46
MAV++ K KKA++VEL ++L+ G V ++++QL+R E+ARRLQWGYKPTY++Q+A++
Sbjct: 1 MAVEA--KQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQ 58
Query: 45 SFAKSLRHMSIATEV 1
+ SLR M IATEV
Sbjct: 59 NLTHSLRQMKIATEV 73
>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
[Thalictrum flavum subsp. glaucum]
gi|51980204|gb|AAU20766.1| (S)-coclaurine
N-methyltransferase [Thalictrum flavum subsp. glaucum]
Length = 361
Score = 80.5 bits (197), Expect = 4e-13
Identities = 38/75 (50%), Positives = 55/75 (73%)
Frame = -2
Query: 225 MAVDSNSKPAKKASVVELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLL 46
MAV+ KKA +VEL ++L+ G V D+E+++L+R ++ RRLQWG K TY++Q+AQL+
Sbjct: 1 MAVEGKQVAPKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLV 60
Query: 45 SFAKSLRHMSIATEV 1
+ SLR M IATEV
Sbjct: 61 NLTHSLRQMKIATEV 75
>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
[Thalictrum flavum subsp. glaucum]
gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
[Thalictrum flavum]
Length = 356
Score = 80.5 bits (197), Expect = 4e-13
Identities = 35/69 (50%), Positives = 54/69 (78%)
Frame = -2
Query: 207 SKPAKKASVVELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLLSFAKSL 28
+K KK +V L +R++ G V DEE+R +M+ ++ +RL+WGYKPT++QQLAQL++FA+SL
Sbjct: 3 TKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSL 62
Query: 27 RHMSIATEV 1
+ M +A EV
Sbjct: 63 KGMEMAEEV 71
>gb|AJD20224.1| coclaurine N-methyltransferase [Sinopodophyllum hexandrum]
Length = 370
Score = 74.7 bits (182), Expect = 2e-11
Identities = 37/64 (57%), Positives = 48/64 (75%)
Frame = -2
Query: 192 KASVVELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLLSFAKSLRHMSI 13
K + EL R+L+ G V D E++QL+R E+ RRL+WGYK T+ QQLAQL+ FA SLR M+I
Sbjct: 22 KTNKAELLRKLELGVVPDHEIKQLIRHELDRRLRWGYKSTHQQQLAQLVDFAHSLRRMNI 81
Query: 12 ATEV 1
A EV
Sbjct: 82 ADEV 85
>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
Length = 351
Score = 73.2 bits (178), Expect = 6e-11
Identities = 32/59 (54%), Positives = 48/59 (81%)
Frame = -2
Query: 177 ELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLLSFAKSLRHMSIATEV 1
EL ++L+ G + ++E+++LMR ++ARRLQWGYK TY++Q+AQ+L SLRHM+IA EV
Sbjct: 8 ELFKKLELGLIPNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEV 66
>gb|AKH61489.1| N-methyltransferase [Expression vector pWCD2353]
Length = 353
Score = 68.6 bits (166), Expect = 2e-09
Identities = 30/59 (50%), Positives = 45/59 (76%)
Frame = -2
Query: 177 ELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLLSFAKSLRHMSIATEV 1
EL R ++ G + D+E+RQL+R E+ +RLQWGYK T+++QL+QLL SL+ M +ATE+
Sbjct: 8 ELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEM 66
>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
somniferum] gi|571330880|gb|AHF27396.1|
S-adenosyl-L-methionine:coclaurine N-methyltransferase
[synthetic construct]
Length = 351
Score = 68.6 bits (166), Expect = 2e-09
Identities = 30/59 (50%), Positives = 45/59 (76%)
Frame = -2
Query: 177 ELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLLSFAKSLRHMSIATEV 1
EL R ++ G + D+E+RQL+R E+ +RLQWGYK T+++QL+QLL SL+ M +ATE+
Sbjct: 8 ELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEM 66
>gb|ADP76529.1| coclaurine N-methyltransferase [Aristolochia fimbriata]
Length = 357
Score = 63.2 bits (152), Expect = 7e-08
Identities = 30/64 (46%), Positives = 46/64 (71%), Gaps = 2/64 (3%)
Frame = -2
Query: 198 AKKASVVELQRRLDEGRVQDEELRQLMRTEIARRLQW--GYKPTYDQQLAQLLSFAKSLR 25
++K + E+ RRL+EG V DEE R+L+R E+ RRL+W KPTY++Q A++++ K+LR
Sbjct: 3 SEKLNKTEMLRRLEEGSVPDEEFRRLIRIELGRRLRWYCQKKPTYEEQTAEIVALVKALR 62
Query: 24 HMSI 13
M I
Sbjct: 63 QMGI 66
>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT [Eschscholzia californica]
gi|226897702|gb|ACO90222.1| tetrahydroprotoberberine
N-methyltransferase [Eschscholzia californica]
Length = 350
Score = 62.8 bits (151), Expect = 9e-08
Identities = 28/63 (44%), Positives = 46/63 (73%)
Frame = -2
Query: 189 ASVVELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLLSFAKSLRHMSIA 10
+S E+ RL +G ++DEEL++L+R + RR++WGYKPT+++QLA L F K L+ M ++
Sbjct: 3 SSAGEIMGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMVMS 62
Query: 9 TEV 1
E+
Sbjct: 63 GEI 65
>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 [Papaver bracteatum]
gi|226897732|gb|ACO90237.1| tetrahydroprotoberberine
N-methyltransferase [Papaver bracteatum]
Length = 358
Score = 60.1 bits (144), Expect = 6e-07
Identities = 28/65 (43%), Positives = 45/65 (69%)
Frame = -2
Query: 195 KKASVVELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLLSFAKSLRHMS 16
KK S E RL +G ++DEEL++L++ + +RLQWGYK ++ +QL+ L F KSL+ M
Sbjct: 8 KKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKKME 67
Query: 15 IATEV 1
++ E+
Sbjct: 68 MSGEI 72
>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT [Papaver somniferum]
gi|67764091|gb|AAY79177.1|
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
cis-N-methyltransferase [Papaver somniferum]
Length = 358
Score = 60.1 bits (144), Expect = 6e-07
Identities = 28/65 (43%), Positives = 45/65 (69%)
Frame = -2
Query: 195 KKASVVELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLLSFAKSLRHMS 16
KK S E RL +G ++DEEL++L++ + +RLQWGYK ++ +QL+ L F KSL+ M
Sbjct: 8 KKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKKME 67
Query: 15 IATEV 1
++ E+
Sbjct: 68 MSGEI 72
>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2 [Papaver
bracteatum] gi|226897730|gb|ACO90236.1| putative
N-methyltransferase [Papaver bracteatum]
Length = 358
Score = 57.8 bits (138), Expect = 3e-06
Identities = 27/65 (41%), Positives = 43/65 (66%)
Frame = -2
Query: 195 KKASVVELQRRLDEGRVQDEELRQLMRTEIARRLQWGYKPTYDQQLAQLLSFAKSLRHMS 16
KK S E RL G + DEEL++L++ ++ +RLQWGYK ++ +QL+ L F SL+ M
Sbjct: 8 KKESAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFINSLKKMG 67
Query: 15 IATEV 1
++ +V
Sbjct: 68 MSGQV 72