BLASTX nr result
ID: Cinnamomum24_contig00026531
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cinnamomum24_contig00026531
(359 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_010917273.1| PREDICTED: DNA polymerase epsilon subunit 2 ... 177 2e-42
ref|XP_010917272.1| PREDICTED: DNA polymerase epsilon subunit 2 ... 177 2e-42
gb|KDO42631.1| hypothetical protein CISIN_1g009713mg [Citrus sin... 176 5e-42
ref|XP_006478101.1| PREDICTED: DNA polymerase epsilon subunit 2-... 176 5e-42
gb|KDO42632.1| hypothetical protein CISIN_1g009713mg [Citrus sin... 176 8e-42
dbj|BAS92403.1| Os05g0160800, partial [Oryza sativa Japonica Group] 175 1e-41
gb|EEC78576.1| hypothetical protein OsI_18564 [Oryza sativa Indi... 175 1e-41
ref|XP_006659486.1| PREDICTED: DNA polymerase epsilon subunit 2-... 175 1e-41
ref|NP_001054723.1| Os05g0160800 [Oryza sativa Japonica Group] g... 175 1e-41
ref|XP_008791857.1| PREDICTED: DNA polymerase epsilon subunit 2 ... 175 1e-41
ref|XP_006441363.1| hypothetical protein CICLE_v10019690mg [Citr... 175 1e-41
ref|XP_006441362.1| hypothetical protein CICLE_v10019690mg [Citr... 174 2e-41
ref|XP_003574603.1| PREDICTED: DNA polymerase epsilon subunit 2 ... 174 2e-41
ref|XP_010276251.1| PREDICTED: DNA polymerase epsilon subunit 2 ... 174 3e-41
ref|XP_007152612.1| hypothetical protein PHAVU_004G144700g [Phas... 174 3e-41
ref|XP_007152611.1| hypothetical protein PHAVU_004G144700g [Phas... 174 3e-41
emb|CDM81836.1| unnamed protein product [Triticum aestivum] 173 4e-41
gb|KQL02122.1| hypothetical protein SETIT_013537mg [Setaria ital... 172 7e-41
ref|XP_012702396.1| PREDICTED: DNA polymerase epsilon subunit 2 ... 172 7e-41
ref|XP_004973646.1| PREDICTED: DNA polymerase epsilon subunit 2 ... 172 7e-41
>ref|XP_010917273.1| PREDICTED: DNA polymerase epsilon subunit 2 isoform X2 [Elaeis
guineensis]
Length = 511
Score = 177 bits (450), Expect = 2e-42
Identities = 84/99 (84%), Positives = 90/99 (90%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFVLMGNFCSRPCNLAF SFSSLR+QFGKLGE+I AHPRLKE SRFLF+PGPDD G
Sbjct: 315 VVPSLFVLMGNFCSRPCNLAFHSFSSLRMQFGKLGEMIEAHPRLKEQSRFLFIPGPDDAG 374
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PSKVLPRC+LPKYL E+LQKHIPNAIF SNPCR + TQ
Sbjct: 375 PSKVLPRCSLPKYLMEDLQKHIPNAIFSSNPCRIKFYTQ 413
>ref|XP_010917272.1| PREDICTED: DNA polymerase epsilon subunit 2 isoform X1 [Elaeis
guineensis]
Length = 530
Score = 177 bits (450), Expect = 2e-42
Identities = 84/99 (84%), Positives = 90/99 (90%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFVLMGNFCSRPCNLAF SFSSLR+QFGKLGE+I AHPRLKE SRFLF+PGPDD G
Sbjct: 315 VVPSLFVLMGNFCSRPCNLAFHSFSSLRMQFGKLGEMIEAHPRLKEQSRFLFIPGPDDAG 374
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PSKVLPRC+LPKYL E+LQKHIPNAIF SNPCR + TQ
Sbjct: 375 PSKVLPRCSLPKYLMEDLQKHIPNAIFSSNPCRIKFYTQ 413
>gb|KDO42631.1| hypothetical protein CISIN_1g009713mg [Citrus sinensis]
Length = 528
Score = 176 bits (447), Expect = 5e-42
Identities = 83/99 (83%), Positives = 89/99 (89%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFV MGNFCS PCNL+F SFSSLRLQFGKLG++I AHPRLKEHSRFLF+PGPDD G
Sbjct: 315 VVPSLFVFMGNFCSHPCNLSFHSFSSLRLQFGKLGQMIAAHPRLKEHSRFLFIPGPDDAG 374
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PS VLPRCALPKYLTEELQK+IPNAIF SNPCR + TQ
Sbjct: 375 PSTVLPRCALPKYLTEELQKYIPNAIFSSNPCRIKFYTQ 413
>ref|XP_006478101.1| PREDICTED: DNA polymerase epsilon subunit 2-like [Citrus sinensis]
Length = 528
Score = 176 bits (447), Expect = 5e-42
Identities = 83/99 (83%), Positives = 89/99 (89%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFV MGNFCS PCNL+F SFSSLRLQFGKLG++I AHPRLKEHSRFLF+PGPDD G
Sbjct: 315 VVPSLFVFMGNFCSHPCNLSFHSFSSLRLQFGKLGQMIAAHPRLKEHSRFLFIPGPDDAG 374
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PS VLPRCALPKYLTEELQK+IPNAIF SNPCR + TQ
Sbjct: 375 PSTVLPRCALPKYLTEELQKYIPNAIFSSNPCRIKFYTQ 413
>gb|KDO42632.1| hypothetical protein CISIN_1g009713mg [Citrus sinensis]
Length = 418
Score = 176 bits (445), Expect = 8e-42
Identities = 83/104 (79%), Positives = 90/104 (86%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFV MGNFCS PCNL+F SFSSLRLQFGKLG++I AHPRLKEHSRFLF+PGPDD G
Sbjct: 315 VVPSLFVFMGNFCSHPCNLSFHSFSSLRLQFGKLGQMIAAHPRLKEHSRFLFIPGPDDAG 374
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQVSRRH 47
PS VLPRCALPKYLTEELQK+IPNAIF SNPCR ++ V H
Sbjct: 375 PSTVLPRCALPKYLTEELQKYIPNAIFSSNPCRLNFLITVQLNH 418
>dbj|BAS92403.1| Os05g0160800, partial [Oryza sativa Japonica Group]
Length = 504
Score = 175 bits (444), Expect = 1e-41
Identities = 83/98 (84%), Positives = 87/98 (88%)
Frame = -2
Query: 355 VPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIGP 176
VPSLFVLMGNFCSRPCNLAF+SF LRLQFGKLGE+I A RLKEHSRFLF+PGPDD GP
Sbjct: 290 VPSLFVLMGNFCSRPCNLAFNSFEELRLQFGKLGEMIAARSRLKEHSRFLFIPGPDDAGP 349
Query: 175 SKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
SK LPRCALPKYLTEELQKHIPNAIF SNPCR + TQ
Sbjct: 350 SKALPRCALPKYLTEELQKHIPNAIFVSNPCRVKFYTQ 387
>gb|EEC78576.1| hypothetical protein OsI_18564 [Oryza sativa Indica Group]
gi|222630291|gb|EEE62423.1| hypothetical protein
OsJ_17214 [Oryza sativa Japonica Group]
Length = 533
Score = 175 bits (444), Expect = 1e-41
Identities = 83/98 (84%), Positives = 87/98 (88%)
Frame = -2
Query: 355 VPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIGP 176
VPSLFVLMGNFCSRPCNLAF+SF LRLQFGKLGE+I A RLKEHSRFLF+PGPDD GP
Sbjct: 319 VPSLFVLMGNFCSRPCNLAFNSFEELRLQFGKLGEMIAARSRLKEHSRFLFIPGPDDAGP 378
Query: 175 SKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
SK LPRCALPKYLTEELQKHIPNAIF SNPCR + TQ
Sbjct: 379 SKALPRCALPKYLTEELQKHIPNAIFVSNPCRVKFYTQ 416
>ref|XP_006659486.1| PREDICTED: DNA polymerase epsilon subunit 2-like [Oryza
brachyantha]
Length = 533
Score = 175 bits (444), Expect = 1e-41
Identities = 83/98 (84%), Positives = 87/98 (88%)
Frame = -2
Query: 355 VPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIGP 176
VPSLFVLMGNFCSRPCNLAF+SF LRLQFGKLGE+I A RLKEHSRFLF+PGPDD GP
Sbjct: 319 VPSLFVLMGNFCSRPCNLAFNSFEELRLQFGKLGEMIAARSRLKEHSRFLFIPGPDDAGP 378
Query: 175 SKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
SK LPRCALPKYLTEELQKHIPNAIF SNPCR + TQ
Sbjct: 379 SKALPRCALPKYLTEELQKHIPNAIFVSNPCRVKFYTQ 416
>ref|NP_001054723.1| Os05g0160800 [Oryza sativa Japonica Group]
gi|51038114|gb|AAT93917.1| unknown protein [Oryza sativa
Japonica Group] gi|55168061|gb|AAV43929.1| unknown
protein [Oryza sativa Japonica Group]
gi|113578274|dbj|BAF16637.1| Os05g0160800 [Oryza sativa
Japonica Group]
Length = 372
Score = 175 bits (444), Expect = 1e-41
Identities = 83/98 (84%), Positives = 87/98 (88%)
Frame = -2
Query: 355 VPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIGP 176
VPSLFVLMGNFCSRPCNLAF+SF LRLQFGKLGE+I A RLKEHSRFLF+PGPDD GP
Sbjct: 158 VPSLFVLMGNFCSRPCNLAFNSFEELRLQFGKLGEMIAARSRLKEHSRFLFIPGPDDAGP 217
Query: 175 SKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
SK LPRCALPKYLTEELQKHIPNAIF SNPCR + TQ
Sbjct: 218 SKALPRCALPKYLTEELQKHIPNAIFVSNPCRVKFYTQ 255
>ref|XP_008791857.1| PREDICTED: DNA polymerase epsilon subunit 2 [Phoenix dactylifera]
Length = 530
Score = 175 bits (443), Expect = 1e-41
Identities = 83/99 (83%), Positives = 89/99 (89%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFVLMGNFCS PCNLAF SFSSLR+QFGKLGE+I A PRLKE SRFLF+PGPDD G
Sbjct: 315 VVPSLFVLMGNFCSHPCNLAFHSFSSLRMQFGKLGEMIEARPRLKERSRFLFIPGPDDAG 374
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PSKVLPRC+LPKYLTE+LQKHIPNAIF SNPCR + TQ
Sbjct: 375 PSKVLPRCSLPKYLTEDLQKHIPNAIFSSNPCRIKFYTQ 413
>ref|XP_006441363.1| hypothetical protein CICLE_v10019690mg [Citrus clementina]
gi|557543625|gb|ESR54603.1| hypothetical protein
CICLE_v10019690mg [Citrus clementina]
Length = 528
Score = 175 bits (443), Expect = 1e-41
Identities = 82/99 (82%), Positives = 89/99 (89%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFV MGNFCS PCNL+F SFSSLRLQFGKLG++I AHPRLKEHSRFLF+PGPDD G
Sbjct: 315 VVPSLFVFMGNFCSHPCNLSFHSFSSLRLQFGKLGQMIAAHPRLKEHSRFLFIPGPDDAG 374
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PS VLPRCALPKYLTEEL+K+IPNAIF SNPCR + TQ
Sbjct: 375 PSTVLPRCALPKYLTEELKKYIPNAIFSSNPCRIKFYTQ 413
>ref|XP_006441362.1| hypothetical protein CICLE_v10019690mg [Citrus clementina]
gi|557543624|gb|ESR54602.1| hypothetical protein
CICLE_v10019690mg [Citrus clementina]
Length = 418
Score = 174 bits (441), Expect = 2e-41
Identities = 82/104 (78%), Positives = 90/104 (86%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFV MGNFCS PCNL+F SFSSLRLQFGKLG++I AHPRLKEHSRFLF+PGPDD G
Sbjct: 315 VVPSLFVFMGNFCSHPCNLSFHSFSSLRLQFGKLGQMIAAHPRLKEHSRFLFIPGPDDAG 374
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQVSRRH 47
PS VLPRCALPKYLTEEL+K+IPNAIF SNPCR ++ V H
Sbjct: 375 PSTVLPRCALPKYLTEELKKYIPNAIFSSNPCRLNFLITVQLNH 418
>ref|XP_003574603.1| PREDICTED: DNA polymerase epsilon subunit 2 [Brachypodium
distachyon] gi|944062996|gb|KQJ98586.1| hypothetical
protein BRADI_3g37840 [Brachypodium distachyon]
Length = 533
Score = 174 bits (441), Expect = 2e-41
Identities = 83/99 (83%), Positives = 87/99 (87%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFVLMGNFCSRPCNLAF+SF LRLQFGKLGE+I A RLKEHSRFLF+PGPDD G
Sbjct: 318 VVPSLFVLMGNFCSRPCNLAFNSFEELRLQFGKLGEMIAARTRLKEHSRFLFIPGPDDAG 377
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PSK LPRCALPKYL EELQKHIPNAIF SNPCR + TQ
Sbjct: 378 PSKALPRCALPKYLIEELQKHIPNAIFVSNPCRVRFYTQ 416
>ref|XP_010276251.1| PREDICTED: DNA polymerase epsilon subunit 2 isoform X2 [Nelumbo
nucifera]
Length = 526
Score = 174 bits (440), Expect = 3e-41
Identities = 83/99 (83%), Positives = 90/99 (90%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFVLMGNFCSRPCNL+F SFSSLR QFGKLGE+IGAH RLKE+S+FLF+PGPDD G
Sbjct: 313 VVPSLFVLMGNFCSRPCNLSFHSFSSLRQQFGKLGEMIGAHQRLKENSQFLFVPGPDDAG 372
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PS VLPRCALPKYLTEELQKHIP+AIF SNPCR + TQ
Sbjct: 373 PSTVLPRCALPKYLTEELQKHIPSAIFSSNPCRIKFYTQ 411
>ref|XP_007152612.1| hypothetical protein PHAVU_004G144700g [Phaseolus vulgaris]
gi|561025921|gb|ESW24606.1| hypothetical protein
PHAVU_004G144700g [Phaseolus vulgaris]
Length = 300
Score = 174 bits (440), Expect = 3e-41
Identities = 82/99 (82%), Positives = 88/99 (88%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFV MGNFCSRPCNL+F S+SSLR+QFGKLGE+I AH RLKE SRFLF+PGPDD G
Sbjct: 87 VVPSLFVFMGNFCSRPCNLSFHSYSSLRMQFGKLGEMIAAHTRLKEQSRFLFIPGPDDAG 146
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PS VLPRCALPKYLTEELQKHIPNAIF SNPCR + TQ
Sbjct: 147 PSTVLPRCALPKYLTEELQKHIPNAIFSSNPCRVKFYTQ 185
>ref|XP_007152611.1| hypothetical protein PHAVU_004G144700g [Phaseolus vulgaris]
gi|561025920|gb|ESW24605.1| hypothetical protein
PHAVU_004G144700g [Phaseolus vulgaris]
Length = 520
Score = 174 bits (440), Expect = 3e-41
Identities = 82/99 (82%), Positives = 88/99 (88%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFV MGNFCSRPCNL+F S+SSLR+QFGKLGE+I AH RLKE SRFLF+PGPDD G
Sbjct: 307 VVPSLFVFMGNFCSRPCNLSFHSYSSLRMQFGKLGEMIAAHTRLKEQSRFLFIPGPDDAG 366
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PS VLPRCALPKYLTEELQKHIPNAIF SNPCR + TQ
Sbjct: 367 PSTVLPRCALPKYLTEELQKHIPNAIFSSNPCRVKFYTQ 405
>emb|CDM81836.1| unnamed protein product [Triticum aestivum]
Length = 533
Score = 173 bits (439), Expect = 4e-41
Identities = 82/99 (82%), Positives = 87/99 (87%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFVLMGNFCSRPCNLAF+SF LRLQFGKLGE+I + RLKEHSRFLF+PGPDD G
Sbjct: 318 VVPSLFVLMGNFCSRPCNLAFNSFEELRLQFGKLGEMIASRTRLKEHSRFLFIPGPDDAG 377
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PSK LPRCALPKYL EELQKHIPNAIF SNPCR + TQ
Sbjct: 378 PSKALPRCALPKYLIEELQKHIPNAIFVSNPCRVKFYTQ 416
>gb|KQL02122.1| hypothetical protein SETIT_013537mg [Setaria italica]
Length = 534
Score = 172 bits (437), Expect = 7e-41
Identities = 83/99 (83%), Positives = 86/99 (86%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFVLMGNFCSRPCNLAFSSF LRLQFGKLGE+I A RLKEHSRFLF+PGPDD G
Sbjct: 319 VVPSLFVLMGNFCSRPCNLAFSSFEELRLQFGKLGEMIAARSRLKEHSRFLFVPGPDDAG 378
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PSK LPRCALPKYL EEL KHIPNAIF SNPCR + TQ
Sbjct: 379 PSKALPRCALPKYLIEELLKHIPNAIFVSNPCRVKFYTQ 417
>ref|XP_012702396.1| PREDICTED: DNA polymerase epsilon subunit 2 isoform X2 [Setaria
italica]
Length = 514
Score = 172 bits (437), Expect = 7e-41
Identities = 83/99 (83%), Positives = 86/99 (86%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFVLMGNFCSRPCNLAFSSF LRLQFGKLGE+I A RLKEHSRFLF+PGPDD G
Sbjct: 299 VVPSLFVLMGNFCSRPCNLAFSSFEELRLQFGKLGEMIAARSRLKEHSRFLFVPGPDDAG 358
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PSK LPRCALPKYL EEL KHIPNAIF SNPCR + TQ
Sbjct: 359 PSKALPRCALPKYLIEELLKHIPNAIFVSNPCRVKFYTQ 397
>ref|XP_004973646.1| PREDICTED: DNA polymerase epsilon subunit 2 isoform X1 [Setaria
italica]
Length = 533
Score = 172 bits (437), Expect = 7e-41
Identities = 83/99 (83%), Positives = 86/99 (86%)
Frame = -2
Query: 358 VVPSLFVLMGNFCSRPCNLAFSSFSSLRLQFGKLGEIIGAHPRLKEHSRFLFLPGPDDIG 179
VVPSLFVLMGNFCSRPCNLAFSSF LRLQFGKLGE+I A RLKEHSRFLF+PGPDD G
Sbjct: 318 VVPSLFVLMGNFCSRPCNLAFSSFEELRLQFGKLGEMIAARSRLKEHSRFLFVPGPDDAG 377
Query: 178 PSKVLPRCALPKYLTEELQKHIPNAIFCSNPCR*SWGTQ 62
PSK LPRCALPKYL EEL KHIPNAIF SNPCR + TQ
Sbjct: 378 PSKALPRCALPKYLIEELLKHIPNAIFVSNPCRVKFYTQ 416