BLASTX nr result
ID: Cinnamomum24_contig00001175
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cinnamomum24_contig00001175
(297 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pdb|2VLC|A Chain A, Crystal Structure Of Natural Cinnamomin (Iso... 91 3e-16
gb|AAK82460.1| type 2 ribosome-inactivating protein cinnamomin I... 91 3e-16
gb|AAK82459.1| type 2 ribosome-inactivating protein cinnamomin I... 90 5e-16
gb|AAF68978.2|AF259548_1 type II ribosome-inactivating protein c... 88 2e-15
gb|AAK82458.1| type 2 ribosome-inactivating protein cinnamomin I... 88 2e-15
gb|AET98835.1| type 2 ribosome-inactivating protein [Cinnamomum ... 84 3e-14
ref|XP_010922360.1| PREDICTED: ricin-like [Elaeis guineensis] gi... 75 2e-11
gb|AAN86130.1|AF249280_1 type 2 ribosome-inactivating protein Ni... 74 4e-11
sp|P33183.2|NIGB_SAMNI RecName: Full=Nigrin b; AltName: Full=Agg... 74 4e-11
gb|AAL04123.1|AF409135_1 ribosome-inactivating protein [Sambucus... 74 4e-11
ref|NP_001295735.1| ricin-like precursor [Jatropha curcas] gi|38... 73 7e-11
gb|AAM46934.1| lectin chain A isoform 3, partial [Viscum coloratum] 73 9e-11
ref|XP_008365107.1| PREDICTED: nigrin b-like [Malus domestica] 71 2e-10
pdb|1PC8|A Chain A, Crystal Structure Of A Novel Form Of Mistlet... 70 6e-10
gb|ABG47419.1| ribosome-inactivating protein [Malus domestica] 70 6e-10
sp|Q6ITZ3.1|ML4_VISAL RecName: Full=Beta-galactoside-specific le... 70 6e-10
ref|XP_009343886.1| PREDICTED: abrin-b-like [Pyrus x bretschneid... 70 7e-10
ref|XP_009363429.1| PREDICTED: abrin-b-like [Pyrus x bretschneid... 70 7e-10
gb|AAR25549.1| lectin precursor [Viscum album] 70 7e-10
ref|XP_008357384.1| PREDICTED: nigrin b-like [Malus domestica] 69 9e-10
>pdb|2VLC|A Chain A, Crystal Structure Of Natural Cinnamomin (Isoform Iii)
gi|223365706|pdb|2VLC|B Chain B, Crystal Structure Of
Natural Cinnamomin (Isoform Iii)
Length = 570
Score = 90.9 bits (224), Expect = 3e-16
Identities = 60/105 (57%), Positives = 67/105 (63%), Gaps = 7/105 (6%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
AVDVTNA VVAY SQSFFL+ED PD L P++ +R PF SYTDLERVA R
Sbjct: 90 AVDVTNAYVVAYRTGSQSFFLREDNPDPAIENLLPDT-KRYTFPFSGSYTDLERVAGERR 148
Query: 131 RNIDLGRTPLANAISNL--EYHDDTSNIARNLIVVNQMVLEAVRF 3
I LG PL NAIS L + +AR+LIVV QMV EAVRF
Sbjct: 149 EEILLGMDPLENAISALWISNLNQQRALARSLIVVIQMVAEAVRF 193
>gb|AAK82460.1| type 2 ribosome-inactivating protein cinnamomin III precursor
[Cinnamomum camphora]
Length = 580
Score = 90.9 bits (224), Expect = 3e-16
Identities = 60/105 (57%), Positives = 67/105 (63%), Gaps = 7/105 (6%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
AVDVTNA VVAY SQSFFL+ED PD L P++ +R PF SYTDLERVA R
Sbjct: 100 AVDVTNAYVVAYRTGSQSFFLREDNPDPAIENLLPDT-KRYTFPFSGSYTDLERVAGERR 158
Query: 131 RNIDLGRTPLANAISNL--EYHDDTSNIARNLIVVNQMVLEAVRF 3
I LG PL NAIS L + +AR+LIVV QMV EAVRF
Sbjct: 159 EEILLGMDPLENAISALWISNLNQQRALARSLIVVIQMVAEAVRF 203
>gb|AAK82459.1| type 2 ribosome-inactivating protein cinnamomin II precursor
[Cinnamomum camphora]
Length = 580
Score = 90.1 bits (222), Expect = 5e-16
Identities = 63/109 (57%), Positives = 69/109 (63%), Gaps = 11/109 (10%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
AVDVTNA VVAY SQSFFL+ED PD L P++ +R PF SYTDLERVA R
Sbjct: 100 AVDVTNAYVVAYRTGSQSFFLREDNPDPAIENLLPDT-KRYTFPFSGSYTDLERVAGELR 158
Query: 131 RNIDLGRTPLANAISNLEYHDDTSN------IARNLIVVNQMVLEAVRF 3
I LG PL NAIS L TSN +AR+LIVV QMV EAVRF
Sbjct: 159 EEILLGMDPLENAISALW----TSNLNQQRALARSLIVVIQMVAEAVRF 203
>gb|AAF68978.2|AF259548_1 type II ribosome-inactivating protein cinnamomin [Cinnamomum
camphora]
Length = 549
Score = 88.2 bits (217), Expect = 2e-15
Identities = 59/105 (56%), Positives = 66/105 (62%), Gaps = 7/105 (6%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
AVDVTNA VVAY SQSFFL+ED PD L P++ +R PF SYTDLE VA R
Sbjct: 68 AVDVTNAYVVAYRTGSQSFFLREDNPDPAIENLLPDT-KRYTFPFSGSYTDLEGVAGERR 126
Query: 131 RNIDLGRTPLANAISNL--EYHDDTSNIARNLIVVNQMVLEAVRF 3
I LG PL NAIS L + +AR+LIVV QMV EAVRF
Sbjct: 127 EEILLGMDPLENAISALWISNLNQQRALARSLIVVIQMVAEAVRF 171
>gb|AAK82458.1| type 2 ribosome-inactivating protein cinnamomin I precursor
[Cinnamomum camphora]
Length = 581
Score = 88.2 bits (217), Expect = 2e-15
Identities = 59/105 (56%), Positives = 66/105 (62%), Gaps = 7/105 (6%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
AVDVTNA VVAY SQSFFL+ED PD L P++ +R PF SYTDLE VA R
Sbjct: 100 AVDVTNAYVVAYRTGSQSFFLREDNPDPAIENLLPDT-KRYTFPFSGSYTDLEGVAGERR 158
Query: 131 RNIDLGRTPLANAISNL--EYHDDTSNIARNLIVVNQMVLEAVRF 3
I LG PL NAIS L + +AR+LIVV QMV EAVRF
Sbjct: 159 EEILLGMDPLENAISALWISNLNQQRALARSLIVVIQMVAEAVRF 203
>gb|AET98835.1| type 2 ribosome-inactivating protein [Cinnamomum camphora]
Length = 585
Score = 84.3 bits (207), Expect = 3e-14
Identities = 55/105 (52%), Positives = 66/105 (62%), Gaps = 7/105 (6%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
A++VT+A VVAY S+SFFL+ED PD LFP++ +R L F +Y DLE A R
Sbjct: 106 ALNVTDAYVVAYRTGSRSFFLREDNPDPAIDNLFPDT-QRQTLRFGGNYNDLEDAAGQRR 164
Query: 131 RNIDLGRTPLANAISNLEYH--DDTSNIARNLIVVNQMVLEAVRF 3
I+LG PL NAIS L D IAR+LIVV QMV EAVRF
Sbjct: 165 DTIELGMEPLENAISTLRVSNTDPDGPIARSLIVVIQMVAEAVRF 209
>ref|XP_010922360.1| PREDICTED: ricin-like [Elaeis guineensis]
gi|743787211|ref|XP_010922361.1| PREDICTED: ricin-like
[Elaeis guineensis]
Length = 570
Score = 75.1 bits (183), Expect = 2e-11
Identities = 53/105 (50%), Positives = 63/105 (60%), Gaps = 7/105 (6%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
AVDV NA VVAY+ QS+F + D PD LF N+ ER L F+SSY L+RVA +R
Sbjct: 105 AVDVINAYVVAYQAGDQSYFFR-DAPDVAFSNLFTNT-ERQTLSFNSSYPALQRVAGEDR 162
Query: 131 RNIDLGRTPLANAISNLEYHDDT--SNIARNLIVVNQMVLEAVRF 3
NIDLG L AIS+L + AR+LIV QMV EA RF
Sbjct: 163 ENIDLGILALEEAISSLHRTSSVQQNTQARSLIVSIQMVSEAARF 207
>gb|AAN86130.1|AF249280_1 type 2 ribosome-inactivating protein Nigrin l precursor [Sambucus
nigra]
Length = 563
Score = 73.9 bits (180), Expect = 4e-11
Identities = 46/102 (45%), Positives = 66/102 (64%), Gaps = 4/102 (3%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIP----DLFPNSGERNILPFDSSYTDLERVANVERR 129
AVDVTN VVA+ G + S+F ++ +LF + ++N L F +Y +LE AN R
Sbjct: 95 AVDVTNLYVVAFSGNANSYFFKDATEVQKSNLFVGT-KQNTLSFTGNYDNLETAANTRRE 153
Query: 128 NIDLGRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVRF 3
+I+LG +PL AI++L YH D ++AR+L+VV QMV EA RF
Sbjct: 154 SIELGPSPLDGAITSL-YHGD--SVARSLLVVIQMVSEAARF 192
>sp|P33183.2|NIGB_SAMNI RecName: Full=Nigrin b; AltName: Full=Agglutinin V; AltName:
Full=SNAV; Contains: RecName: Full=Nigrin b A chain;
AltName: Full=rRNA N-glycosidase; Contains: RecName:
Full=Nigrin b B chain; Flags: Precursor [Sambucus nigra]
gi|1754981|gb|AAB39475.1| ribosome inactivating protein
precursor [Sambucus nigra] gi|1589093|prf||2210286A
agglutinin V
Length = 563
Score = 73.9 bits (180), Expect = 4e-11
Identities = 46/102 (45%), Positives = 66/102 (64%), Gaps = 4/102 (3%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIP----DLFPNSGERNILPFDSSYTDLERVANVERR 129
AVDVTN VVA+ G + S+F ++ +LF + ++N L F +Y +LE AN R
Sbjct: 95 AVDVTNLYVVAFSGNANSYFFKDATEVQKSNLFVGT-KQNTLSFTGNYDNLETAANTRRE 153
Query: 128 NIDLGRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVRF 3
+I+LG +PL AI++L YH D ++AR+L+VV QMV EA RF
Sbjct: 154 SIELGPSPLDGAITSL-YHGD--SVARSLLVVIQMVSEAARF 192
>gb|AAL04123.1|AF409135_1 ribosome-inactivating protein [Sambucus nigra]
Length = 563
Score = 73.9 bits (180), Expect = 4e-11
Identities = 46/102 (45%), Positives = 66/102 (64%), Gaps = 4/102 (3%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIP----DLFPNSGERNILPFDSSYTDLERVANVERR 129
AVDVTN VVA+ G + S+F ++ +LF + ++N L F +Y +LE AN R
Sbjct: 95 AVDVTNLYVVAFSGNANSYFFKDATEVQKSNLFVGT-KQNTLSFTGNYDNLETAANTRRE 153
Query: 128 NIDLGRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVRF 3
+I+LG +PL AI++L YH D ++AR+L+VV QMV EA RF
Sbjct: 154 SIELGPSPLDGAITSL-YHGD--SVARSLLVVIQMVSEAARF 192
>ref|NP_001295735.1| ricin-like precursor [Jatropha curcas] gi|388564818|gb|AFK73425.1|
type 2 ribosomal inactivating protein [Jatropha curcas]
gi|643725759|gb|KDP34680.1| hypothetical protein
JCGZ_10885 [Jatropha curcas]
Length = 551
Score = 73.2 bits (178), Expect = 7e-11
Identities = 46/101 (45%), Positives = 63/101 (62%), Gaps = 3/101 (2%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIP--DLFPNSGERNILPFDSSYTDLERVANVERRNI 123
A+DV A VVAY ++QS+F + + +LF N ++N LPF SSY +LE + R I
Sbjct: 100 ALDVATAGVVAYGFQNQSYFFNDKLAFSNLF-NDTKQNTLPFGSSYGELENSTGMPRSKI 158
Query: 122 DLGRTPLANAISNLEYHD-DTSNIARNLIVVNQMVLEAVRF 3
DLG PL A+SN+ ++ T ++A LIVV QMV EA RF
Sbjct: 159 DLGLLPLNEAVSNIVANNIKTEDLASCLIVVMQMVSEAARF 199
>gb|AAM46934.1| lectin chain A isoform 3, partial [Viscum coloratum]
Length = 251
Score = 72.8 bits (177), Expect = 9e-11
Identities = 48/104 (46%), Positives = 64/104 (61%), Gaps = 6/104 (5%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
A+DVTN VVAY+ QS+FL+ D PD LF + R+ LPF+ SY DLER A R
Sbjct: 71 AIDVTNLYVVAYQAGDQSYFLR-DAPDGAERHLFTGT-TRSSLPFNGSYADLERYAG-HR 127
Query: 131 RNIDLGRTPLANAISNLEYHDDTSNI-ARNLIVVNQMVLEAVRF 3
I LGR PL ++S L+Y ++ A ++I+V QM+ EA RF
Sbjct: 128 DRIPLGREPLIRSVSALDYPGGSTRAQASSIIIVIQMISEAARF 171
>ref|XP_008365107.1| PREDICTED: nigrin b-like [Malus domestica]
Length = 548
Score = 71.2 bits (173), Expect = 2e-10
Identities = 43/102 (42%), Positives = 59/102 (57%), Gaps = 4/102 (3%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD----LFPNSGERNILPFDSSYTDLERVANVERR 129
A+DV NA+VVAY R+ + D PD + N LPF S+Y DLE+ A R
Sbjct: 86 AIDVVNASVVAYRARAARPYFLADAPDEALDILFNDTRGFFLPFTSNYXDLEKAAEKSRD 145
Query: 128 NIDLGRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVRF 3
I LG TPL NAI++L ++ ++ A +L+V+ Q V EA RF
Sbjct: 146 KIPLGLTPLHNAITSL-WNXESEEAAVSLLVIIQTVFEAARF 186
>pdb|1PC8|A Chain A, Crystal Structure Of A Novel Form Of Mistletoe Lectin From
Himalayan Viscum Album L. At 3.8a Resolution
gi|51247662|pdb|1TFM|A Chain A, Crystal Structure Of A
Ribosome Inactivating Protein In Its Naturally Inhibited
Form gi|62738635|pdb|1YF8|A Chain A, Crystal Structure
Of Himalayan Mistletoe Rip Reveals The Presence Of A
Natural Inhibitor And A New Functionally Active
Sugar-Binding Site
Length = 240
Score = 70.1 bits (170), Expect = 6e-10
Identities = 41/98 (41%), Positives = 60/98 (61%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPDLFPNSGERNILPFDSSYTDLERVANVERRNIDL 117
A+DVTN VVAY+ SQS+FL LF + R+ LPF+ SY DLE+ A R+ I L
Sbjct: 68 AIDVTNLYVVAYQAGSQSYFLSGPGTHLFTGT-TRSSLPFNGSYPDLEQYAG-HRKQIPL 125
Query: 116 GRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVRF 3
G L +++ L + +T AR+++++ QM+ EA RF
Sbjct: 126 GIDQLIQSVTALRFPGNTRTQARSILILIQMISEAARF 163
>gb|ABG47419.1| ribosome-inactivating protein [Malus domestica]
Length = 189
Score = 70.1 bits (170), Expect = 6e-10
Identities = 43/101 (42%), Positives = 57/101 (56%), Gaps = 4/101 (3%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD----LFPNSGERNILPFDSSYTDLERVANVERR 129
A+DV NA+VVAY R+ + D PD + N LPF S+Y DLE+ A R
Sbjct: 86 AIDVVNASVVAYRARAARPYFLADAPDEALDILFNDTRGFFLPFTSNYLDLEKAAEKSRE 145
Query: 128 NIDLGRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVR 6
I LG TPL NAI++L H ++ A +L+V+ Q V EA R
Sbjct: 146 KIPLGLTPLHNAITSLWNH-ESEEAAVSLLVIIQTVFEAAR 185
>sp|Q6ITZ3.1|ML4_VISAL RecName: Full=Beta-galactoside-specific lectin 4; AltName:
Full=Beta-galactoside-specific lectin IV; Contains:
RecName: Full=Beta-galactoside-specific lectin 4 chain
A; AltName: Full=Beta-galactoside-specific lectin IV
chain A; AltName: Full=ML-4 A; AltName: Full=ML-IV A;
AltName: Full=rRNA N-glycosidase; Contains: RecName:
Full=Beta-galactoside-specific lectin 4 chain B;
AltName: Full=Beta-galactoside-specific lectin IV chain
B; AltName: Full=ML-4B; AltName: Full=ML-IV B; Flags:
Precursor [Viscum album] gi|47716663|gb|AAT37532.1|
Himalayan mistletoe ribosome-inactivating protein
precursor [Viscum album]
Length = 520
Score = 70.1 bits (170), Expect = 6e-10
Identities = 41/98 (41%), Positives = 60/98 (61%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPDLFPNSGERNILPFDSSYTDLERVANVERRNIDL 117
A+DVTN VVAY+ SQS+FL LF + R+ LPF+ SY DLE+ A R+ I L
Sbjct: 68 AIDVTNLYVVAYQAGSQSYFLSGPGTHLFTGT-TRSSLPFNGSYPDLEQYAG-HRKQIPL 125
Query: 116 GRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVRF 3
G L +++ L + +T AR+++++ QM+ EA RF
Sbjct: 126 GIDQLIQSVTALRFPGNTRTQARSILILIQMISEAARF 163
>ref|XP_009343886.1| PREDICTED: abrin-b-like [Pyrus x bretschneideri]
Length = 194
Score = 69.7 bits (169), Expect = 7e-10
Identities = 46/101 (45%), Positives = 60/101 (59%), Gaps = 4/101 (3%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQED----IPDLFPNSGERNILPFDSSYTDLERVANVERR 129
A+D NA VVAY G +S+FL++ + LFP + LPF SSYTDLE+ R
Sbjct: 50 AIDKENANVVAYVGGDKSYFLKDAPVAALNTLFPPTTSF-YLPFTSSYTDLEKAGRKSRW 108
Query: 128 NIDLGRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVR 6
I LG TPL NAI+ L + S A++L+V QMVLEA +
Sbjct: 109 EIPLGLTPLDNAITAL-WEGGDSKAAQSLLVTTQMVLEAAQ 148
>ref|XP_009363429.1| PREDICTED: abrin-b-like [Pyrus x bretschneideri]
Length = 194
Score = 69.7 bits (169), Expect = 7e-10
Identities = 46/101 (45%), Positives = 60/101 (59%), Gaps = 4/101 (3%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQED----IPDLFPNSGERNILPFDSSYTDLERVANVERR 129
A+D NA VVAY G +S+FL++ + LFP + LPF SSYTDLE+ R
Sbjct: 50 AIDKENANVVAYVGGDKSYFLKDAPVAALNTLFPPTTSF-YLPFTSSYTDLEKAGRKSRW 108
Query: 128 NIDLGRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVR 6
I LG TPL NAI+ L + S A++L+V QMVLEA +
Sbjct: 109 EIPLGLTPLDNAITAL-WEGGDSKAAQSLLVTTQMVLEAAQ 148
>gb|AAR25549.1| lectin precursor [Viscum album]
Length = 567
Score = 69.7 bits (169), Expect = 7e-10
Identities = 46/104 (44%), Positives = 62/104 (59%), Gaps = 6/104 (5%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
A+DVTN VVAY+ QS++L+ D PD LF + R+ LPF+ SY DLER A R
Sbjct: 102 AIDVTNLYVVAYQAAGQSYYLR-DAPDGAERHLFTGT-TRSSLPFNGSYADLERYAG-HR 158
Query: 131 RNIDLGRTPLANAISNLEYHDDTSNI-ARNLIVVNQMVLEAVRF 3
I LGR PL ++S L Y ++ A ++I+V QM+ E RF
Sbjct: 159 DRIPLGREPLLRSVSALHYPGGSTRAQASSIIIVIQMISEVARF 202
>ref|XP_008357384.1| PREDICTED: nigrin b-like [Malus domestica]
Length = 548
Score = 69.3 bits (168), Expect = 9e-10
Identities = 45/103 (43%), Positives = 58/103 (56%), Gaps = 5/103 (4%)
Frame = -2
Query: 296 AVDVTNAAVVAYEGRSQSFFLQEDIPD-----LFPNSGERNILPFDSSYTDLERVANVER 132
A+DV N +VVAY R+ + D PD LF N LPF S+Y DLE+ A R
Sbjct: 86 AIDVVNXSVVAYRARAARPYFLADAPDEALDXLF-NDTRGFFLPFTSNYXDLEKAAEKSR 144
Query: 131 RNIDLGRTPLANAISNLEYHDDTSNIARNLIVVNQMVLEAVRF 3
I LG TPL NAI++L H ++ A +L+V+ Q V EA RF
Sbjct: 145 DKIPLGLTPLHNAITSLWNH-ESEEAAVSLLVIIQTVFEAARF 186