BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cinnamomum23_contig00024086
         (572 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   232   8e-59
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]      221   2e-55
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   218   1e-54
ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   202   1e-49
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   201   2e-49
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ...   197   3e-48
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   197   4e-48
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   196   5e-48
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   196   5e-48
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   195   1e-47
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   194   2e-47
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   194   2e-47
gb|AFK73713.1| cytochrome P450 [Papaver somniferum]                   189   7e-46
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum...   186   6e-45
emb|CDP15158.1| unnamed protein product [Coffea canephora]            175   1e-41
emb|CDP15153.1| unnamed protein product [Coffea canephora]            175   1e-41
emb|CDP22276.1| unnamed protein product [Coffea canephora]            175   1e-41
ref|XP_009610421.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   171   2e-40
ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   170   5e-40
ref|XP_009609252.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   169   6e-40

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  232 bits (592), Expect = 8e-59
 Identities = 112/193 (58%), Positives = 146/193 (75%), Gaps = 3/193 (1%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAP---HVELMKLARTHGPLMLVKFGAQPVI 402
           +  L    KNLPPGP PWP+IGN+  + T      H+ L  +ARTHGP+M++  G QP +
Sbjct: 18  ILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTV 77

Query: 401 IASTPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFS 222
           +AST  AA EILK +DRI S R +  S R+K+HIK+S+VW+DCTD WK LR+I RTE+FS
Sbjct: 78  MASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFS 137

Query: 221 TKMLDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGEKG 42
            KML AQ  VRE+KVAE+++F+R ++G+ V I++FVFGTLLNILG+VVFSKDVF YG++ 
Sbjct: 138 PKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYGDET 197

Query: 41  DKVGMQRLIREML 3
           DK G+Q LIREML
Sbjct: 198 DKGGIQNLIREML 210


>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
          Length = 483

 Score =  221 bits (563), Expect = 2e-55
 Identities = 114/193 (59%), Positives = 146/193 (75%), Gaps = 3/193 (1%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGA--PHVELMKLARTHGPLMLVKFGAQPVII 399
           + F P S KNLPPGPKPWPIIGN+ ++ + A   HV L  +ARTHGP+M++  G QP  +
Sbjct: 18  ILFKPRS-KNLPPGPKPWPIIGNIPALFSSAYPMHVALTNMARTHGPIMILWLGTQPTAV 76

Query: 398 ASTPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFST 219
           AST  AA EILK +DR  SGR V  S R+K+HIK+S+VW DC++ WK LR+I RTE+FS 
Sbjct: 77  ASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCSEYWKLLRKIVRTEIFSP 136

Query: 218 KMLDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQY-GEKG 42
           KML  Q  VRE+KVAE+V F++G++G+ V I++ VFGTLLNILG+V+FSKDVF+Y G  G
Sbjct: 137 KMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVGADG 196

Query: 41  DKVGMQRLIREML 3
           DK  MQ LI+EML
Sbjct: 197 DK--MQTLIKEML 207


>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Nelumbo nucifera]
          Length = 485

 Score =  218 bits (556), Expect = 1e-54
 Identities = 102/191 (53%), Positives = 144/191 (75%), Gaps = 1/191 (0%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           + FL  S  NLPPGP  WPIIG +L  L   PHVEL KLA+  GPLML+KFG +PV++AS
Sbjct: 18  VLFLKPSPNNLPPGPFSWPIIGTLLPKLKKQPHVELSKLAQRFGPLMLLKFGVEPVVVAS 77

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKM 213
           +  AA E+LK  DR+LSGRF PHS+R+K +I+HS+VW DCTD WK +R++ RTELFSTKM
Sbjct: 78  SHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWKMVRKVWRTELFSTKM 137

Query: 212 LDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGEKGD-K 36
           LD Q   RE+KV+E++ F+  ++G+ V   + +FG++LNILG ++FSKDV+ + ++ D  
Sbjct: 138 LDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALIFSKDVYDFEDRTDNN 197

Query: 35  VGMQRLIREML 3
           +GM+ +IR+++
Sbjct: 198 LGMKGMIRQLM 208


>ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Nelumbo nucifera]
          Length = 486

 Score =  202 bits (513), Expect = 1e-49
 Identities = 98/191 (51%), Positives = 142/191 (74%), Gaps = 1/191 (0%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           LF  PSS+K LPPGP  WPIIG  L   T  P++EL KLA+ +GPLML +FG + V++AS
Sbjct: 19  LFLKPSSKK-LPPGPFSWPIIGTQLPSPTMKPNLELFKLAQRYGPLMLFRFGFENVVVAS 77

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKM 213
              AA E+LK  DR+LSGRF  +S+R+K +I++S+VW DCTD WK +R+I RTELFSTKM
Sbjct: 78  NHVAAMEVLKNQDRVLSGRFKANSVRVKGYIEYSMVWADCTDYWKMVRKILRTELFSTKM 137

Query: 212 LDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGEKGD-K 36
           LD    VRE+KV+E++ F+R ++G+ V   + +FG +LN+LG +++SKDV+ + +K D  
Sbjct: 138 LDVHAHVREEKVSELMKFLRRKEGEEVNFVDVIFGCILNMLGALIYSKDVYDFEDKTDIN 197

Query: 35  VGMQRLIREML 3
           +GM+ +IR+++
Sbjct: 198 LGMKGMIRQLM 208


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
           isozyme 2; AltName: Full=Cytochrome P450 80B2 [Coptis
           japonica] gi|9971208|dbj|BAB12433.1|
           (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  201 bits (511), Expect = 2e-49
 Identities = 96/171 (56%), Positives = 131/171 (76%), Gaps = 1/171 (0%)
 Frame = -1

Query: 569 FFLPSSR-KNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           F L  SR KNLPPGP+P PI+GN+L  L   PH E  KLA+ +G L  +K G+Q V++AS
Sbjct: 19  FILRDSRPKNLPPGPRPSPIVGNLLQ-LGDKPHAEFAKLAQKYGELFSLKLGSQTVVVAS 77

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKM 213
           +PAAA EILK +D+ILSGR+V  S R+K H+++S+VW++C DNWK LR++ RTELF+ KM
Sbjct: 78  SPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKM 137

Query: 212 LDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVF 60
           +++Q  +RE K  EMV F+RG++G+ V I E VFGTL+NI G+++FSKDVF
Sbjct: 138 IESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVF 188


>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
           californica [Expression vector pWCD2353]
          Length = 490

 Score =  197 bits (501), Expect = 3e-48
 Identities = 89/175 (50%), Positives = 131/175 (74%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           L F  S  KNLPPGPKPWPI+GN+L  L   PH +  +LA+T+G +  +K G + V++AS
Sbjct: 19  LLFGSSGHKNLPPGPKPWPIVGNLLQ-LGEKPHAQFAELAQTYGDIFTLKMGTETVVVAS 77

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKM 213
           T +AA+EILK +DRILS R+V  S R+K H+++S+VW+DCT+ WK LR++ RTELF+ KM
Sbjct: 78  TSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKM 137

Query: 212 LDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGE 48
           +++Q  VREKK  EMV ++  + G+ V I E +FGTL+NI G+++FS+++F+ G+
Sbjct: 138 IESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGD 192


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1, partial
           [Eschscholzia californica] gi|3127027|gb|AAC39452.1|
           (S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
           californica]
          Length = 487

 Score =  197 bits (500), Expect = 4e-48
 Identities = 89/175 (50%), Positives = 131/175 (74%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           L F  S  KNLPPGPKPWPI+GN+L  L   PH +  +LA+T+G +  +K G + V++AS
Sbjct: 18  LLFGGSGHKNLPPGPKPWPIVGNLLQ-LGEKPHAQFAELAQTYGDIFTLKMGTETVVVAS 76

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKM 213
           T +AA+EILK +DRILS R+V  S R+K H+++S+VW+DCT+ WK LR++ RTELF+ KM
Sbjct: 77  TSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKM 136

Query: 212 LDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGE 48
           +++Q  VREKK  EMV ++  + G+ V I E +FGTL+NI G+++FS+++F+ G+
Sbjct: 137 IESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGD 191


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
           AltName: Full=Cytochrome P450 80B2 [Eschscholzia
           californica] gi|3127029|gb|AAC39453.1|
           (S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
           californica]
          Length = 488

 Score =  196 bits (499), Expect = 5e-48
 Identities = 89/174 (51%), Positives = 130/174 (74%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           L F  S  KNLPPGPKPWPI+GN+L  L   PH +  +LA+T+G +  +K G + V++AS
Sbjct: 19  LLFGSSGHKNLPPGPKPWPIVGNLLQ-LGEKPHAQFAELAQTYGDIFTLKMGTETVVVAS 77

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKM 213
           T +AA+EILK +DRILS R+V  S R+K H+++S+VW+DCT+ WK LR++ RTELF+ KM
Sbjct: 78  TSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKM 137

Query: 212 LDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYG 51
           +++Q  VREKK  EMV ++  + G+ V I E +FGTL+NI G+++FS+++F+ G
Sbjct: 138 IESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELG 191


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  196 bits (499), Expect = 5e-48
 Identities = 93/191 (48%), Positives = 135/191 (70%), Gaps = 1/191 (0%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           L F  SS K LPPGPKPWPI+GN+L  L   PH +  +LA+T+G L  +K G++ V++AS
Sbjct: 18  LIFRDSSPKGLPPGPKPWPIVGNLLQ-LGEKPHSQFAELAKTYGDLFTLKLGSETVVVAS 76

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKM 213
           TP AA+EILK +DR+LSGR+V  S R+K H+++S+VW++C + WK LR++ R ELF+ KM
Sbjct: 77  TPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKM 136

Query: 212 LDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYG-EKGDK 36
           +++Q  +RE K  EMV F++   G  V I E VFGTL+NI G+++FS+++F+ G E    
Sbjct: 137 IESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGS 196

Query: 35  VGMQRLIREML 3
           V M+  +  ML
Sbjct: 197 VEMKEHLWRML 207


>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  195 bits (496), Expect = 1e-47
 Identities = 94/191 (49%), Positives = 135/191 (70%), Gaps = 1/191 (0%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           L F  SS K LPPGPKPWPI+GN+L  L   PH +  +LA T+G L  +K G++ V++AS
Sbjct: 13  LIFRDSSPKGLPPGPKPWPIVGNLLQ-LGEKPHSQFAQLAETYGDLFSLKLGSETVVVAS 71

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKM 213
           TP AA+EILK +DR+LSGR+V  S R+K H+++S+VW++C + WK LR++ RTELF+ KM
Sbjct: 72  TPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKM 131

Query: 212 LDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYG-EKGDK 36
           +++Q  VRE K  EMV +++   G  V I E VFGTL+NI G+++FS+++F+ G E    
Sbjct: 132 IESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGS 191

Query: 35  VGMQRLIREML 3
           V M+  +  ML
Sbjct: 192 VEMKEHLWRML 202


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  194 bits (494), Expect = 2e-47
 Identities = 92/173 (53%), Positives = 130/173 (75%), Gaps = 2/173 (1%)
 Frame = -1

Query: 572 LFFL--PSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVII 399
           LFF+     +KNLPPGP+P PI+GN+L  L   P  E  KLA+ +G L  +K G+Q V++
Sbjct: 17  LFFILRDPRQKNLPPGPRPLPIVGNLLQ-LGDKPRAEFAKLAQKYGQLFSLKLGSQTVVV 75

Query: 398 ASTPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFST 219
           AS+PAAA E+LK +D+ILSGR+V  S R+K H+++S+VW++C DNWK LR++ RTELF+ 
Sbjct: 76  ASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTP 135

Query: 218 KMLDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVF 60
           KM+++Q  +RE K  EMV F+R ++G+ V I E VFGTL+NI G+++FSKDVF
Sbjct: 136 KMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVF 188


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  194 bits (493), Expect = 2e-47
 Identities = 93/191 (48%), Positives = 135/191 (70%), Gaps = 1/191 (0%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           L F  SS K LPPGPKPWPI+GN+L  L   PH +  +LA T+G L  +K G++ V++AS
Sbjct: 13  LIFRDSSPKGLPPGPKPWPIVGNLLQ-LGEKPHSQFAQLAETYGDLFSLKLGSETVVVAS 71

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKM 213
           TP AA+EILK +DR+LSGR+V  S R+K H+++S+VW++C + WK LR++ RT+LF+ KM
Sbjct: 72  TPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKM 131

Query: 212 LDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYG-EKGDK 36
           +++Q  VRE K  EMV +++   G  V I E VFGTL+NI G+++FS+++F+ G E    
Sbjct: 132 IESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGS 191

Query: 35  VGMQRLIREML 3
           V M+  +  ML
Sbjct: 192 VEMKEHLWRML 202


>gb|AFK73713.1| cytochrome P450 [Papaver somniferum]
          Length = 440

 Score =  189 bits (480), Expect = 7e-46
 Identities = 90/186 (48%), Positives = 131/186 (70%), Gaps = 1/186 (0%)
 Frame = -1

Query: 557 SSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIASTPAAA 378
           SS   LPPGPKPWPI+GN+L  L   PH +  +LA T+G L  +K G Q V++ASTP AA
Sbjct: 24  SSPNGLPPGPKPWPIVGNLLQ-LGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAA 82

Query: 377 TEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFSTKMLDAQL 198
           +E+LKA+DR L GR+V  S R+KNH+++S+VW +C + WK LR++ RT+LF+ KM++ Q 
Sbjct: 83  SEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNETWKKLRKVCRTQLFTQKMIENQA 142

Query: 197 LVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYG-EKGDKVGMQR 21
            VRE K  EMV +++  +G  V I E +FGTL+N+ G+++FS+D+F+ G E    + M++
Sbjct: 143 EVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQ 202

Query: 20  LIREML 3
            I  ML
Sbjct: 203 HIWRML 208


>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
           glaucum]
          Length = 491

 Score =  186 bits (472), Expect = 6e-45
 Identities = 89/173 (51%), Positives = 125/173 (72%), Gaps = 2/173 (1%)
 Frame = -1

Query: 572 LFFL--PSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVII 399
           +FF+   S  K+LPPGP+P PI+GN+L  L   PH E  KLA  +G L  +K G+Q V++
Sbjct: 18  IFFILCDSRNKDLPPGPRPSPIVGNLLQ-LGEKPHAEFAKLAEKYGELFTLKLGSQTVVV 76

Query: 398 ASTPAAATEILKANDRILSGRFVPHSLRMKNHIKHSVVWTDCTDNWKTLRRIARTELFST 219
           AS+PAAA EILK  D+ILSGR+V  S R+  H+ +S+VW++C +NWK LR++ RTELFS 
Sbjct: 77  ASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNENWKLLRKVCRTELFSP 136

Query: 218 KMLDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVF 60
           KM+++Q  +RE K  +MV F+R ++ + V I E VF TL+NI G+++FSKDVF
Sbjct: 137 KMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLIFSKDVF 189


>emb|CDP15158.1| unnamed protein product [Coffea canephora]
          Length = 496

 Score =  175 bits (443), Expect = 1e-41
 Identities = 89/193 (46%), Positives = 130/193 (67%), Gaps = 3/193 (1%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           L ++ S  ++LPPGPKPWPI+GN L  +   PHV L +LA+ +GPL+ ++ G+Q V++ S
Sbjct: 24  LNYIKSRNQSLPPGPKPWPIVGN-LPQIGSKPHVALAQLAQDYGPLISLRLGSQLVVVGS 82

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKH--SVVWT-DCTDNWKTLRRIARTELFS 222
           TPAAATEILK +DRILSGR VPH    K+ + +  SV WT +CTD WK LR + ++E+  
Sbjct: 83  TPAAATEILKTHDRILSGRHVPHVSYAKSPLMNYVSVGWTYECTDQWKFLRTLCKSEILG 142

Query: 221 TKMLDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGEKG 42
            K+++ Q  +RE+K  E+V F+  ++G+ + I E VF ++ N LG + FSKD   Y E  
Sbjct: 143 AKVIENQSHLREQKANELVQFLVSKEGQRIKIAEVVFVSVFNFLGQIFFSKDFLSYDEVE 202

Query: 41  DKVGMQRLIREML 3
           +  GM  LIRE++
Sbjct: 203 NGGGMSELIREVM 215


>emb|CDP15153.1| unnamed protein product [Coffea canephora]
          Length = 496

 Score =  175 bits (443), Expect = 1e-41
 Identities = 89/193 (46%), Positives = 130/193 (67%), Gaps = 3/193 (1%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           L ++ S  ++LPPGPKPWPI+GN L  +   PHV L +LA+ +GPL+ ++ G+Q V++ S
Sbjct: 24  LNYIKSRNQSLPPGPKPWPIVGN-LPQIGSKPHVALAQLAQDYGPLISLRLGSQLVVVGS 82

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKH--SVVWT-DCTDNWKTLRRIARTELFS 222
           TPAAATEILK +DRILSGR VPH    K+ + +  SV WT +CTD WK LR + ++E+  
Sbjct: 83  TPAAATEILKTHDRILSGRHVPHVSYAKSPLMNYVSVGWTYECTDQWKFLRTLCKSEILG 142

Query: 221 TKMLDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGEKG 42
            K+++ Q  +RE+K  E+V F+  ++G+ + I E VF ++ N LG + FSKD   Y E  
Sbjct: 143 AKVIENQSHLREQKANELVQFLVSKEGQRIKIAEVVFVSVFNFLGQIFFSKDFLSYDEVE 202

Query: 41  DKVGMQRLIREML 3
           +  GM  LIRE++
Sbjct: 203 NGGGMSELIREVM 215


>emb|CDP22276.1| unnamed protein product [Coffea canephora]
          Length = 312

 Score =  175 bits (443), Expect = 1e-41
 Identities = 89/193 (46%), Positives = 130/193 (67%), Gaps = 3/193 (1%)
 Frame = -1

Query: 572 LFFLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIAS 393
           L ++ S  ++LPPGPKPWPI+GN L  +   PHV L +LA+ +GPL+ ++ G+Q V++ S
Sbjct: 24  LNYIKSRNQSLPPGPKPWPIVGN-LPQIGSKPHVALAQLAQDYGPLISLRLGSQLVVVGS 82

Query: 392 TPAAATEILKANDRILSGRFVPHSLRMKNHIKH--SVVWT-DCTDNWKTLRRIARTELFS 222
           TPAAATEILK +DRILSGR VPH    K+ + +  SV WT +CTD WK LR + ++E+  
Sbjct: 83  TPAAATEILKTHDRILSGRHVPHVSYAKSPLMNYVSVGWTYECTDQWKFLRTLCKSEILG 142

Query: 221 TKMLDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGEKG 42
            K+++ Q  +RE+K  E+V F+  ++G+ + I E VF ++ N LG + FSKD   Y E  
Sbjct: 143 AKVIENQSHLREQKANELVQFLVSKEGQRIKIAEVVFVSVFNFLGQIFFSKDFLSYDEVE 202

Query: 41  DKVGMQRLIREML 3
           +  GM  LIRE++
Sbjct: 203 NGGGMSELIREVM 215


>ref|XP_009610421.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Nicotiana tomentosiformis]
          Length = 489

 Score =  171 bits (434), Expect = 2e-40
 Identities = 87/191 (45%), Positives = 129/191 (67%), Gaps = 3/191 (1%)
 Frame = -1

Query: 566 FLPSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIASTP 387
           F+P S  NLPPGP+PWPI+GN+L  L   PH+ + + A+ HGPL+ ++ G Q V+IAS+P
Sbjct: 23  FIPKSG-NLPPGPRPWPILGNILQ-LGKNPHISMTQFAKIHGPLICLRLGTQLVVIASSP 80

Query: 386 AAATEILKANDRILSGRFVPHSLRMKNHI--KHSVVWT-DCTDNWKTLRRIARTELFSTK 216
            +A EILK  DR+LS R VP     K  +  +HS+VW+ D T++WK+LR   RT LFS K
Sbjct: 81  DSAAEILKTQDRLLSARSVPRVAPYKLSVIDQHSIVWSSDLTNHWKSLRAFCRTHLFSAK 140

Query: 215 MLDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGEKGDK 36
            +++Q  +REKKV EM+ F+R   GK V I+E +F T+LN LG++ F+K++     +G+ 
Sbjct: 141 AVESQADLREKKVFEMIEFLRSNKGKTVKISEILFATILNTLGNLFFTKNLCDLDSEGNT 200

Query: 35  VGMQRLIREML 3
            G++ +IR  +
Sbjct: 201 SGIKHVIRRFV 211


>ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           isoform X1 [Nicotiana sylvestris]
           gi|698520618|ref|XP_009757124.1| PREDICTED: probable
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 isoform
           X2 [Nicotiana sylvestris]
          Length = 489

 Score =  170 bits (430), Expect = 5e-40
 Identities = 84/184 (45%), Positives = 127/184 (69%), Gaps = 3/184 (1%)
 Frame = -1

Query: 545 NLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQPVIIASTPAAATEIL 366
           NLPPGP+PWPI+GN+L  L   PH+ + + A+ HGPL+ ++ G Q V+IAS+P +A EIL
Sbjct: 29  NLPPGPRPWPILGNILQ-LGKNPHISMTQFAKIHGPLISLRLGTQLVVIASSPDSAAEIL 87

Query: 365 KANDRILSGRFVPHSLRMKNHI--KHSVVWT-DCTDNWKTLRRIARTELFSTKMLDAQLL 195
           K  DR+LS R VP     K  +  +HS+VW+ D +++WK+LR   RT LFS K +++Q  
Sbjct: 88  KTQDRLLSARSVPRVAPYKLSVVDQHSLVWSSDLSNHWKSLRAFCRTHLFSAKAVESQAA 147

Query: 194 VREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQYGEKGDKVGMQRLI 15
           +REKKV EM+ F+R + GK V I+E +F T+LN LG++ F+KD+     +G+  G++ +I
Sbjct: 148 LREKKVFEMIEFLRSKKGKTVKISEIMFATILNTLGNLFFTKDLCDLDYEGNTSGIKHVI 207

Query: 14  REML 3
           R+ +
Sbjct: 208 RKFV 211


>ref|XP_009609252.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Nicotiana tomentosiformis]
          Length = 495

 Score =  169 bits (429), Expect = 6e-40
 Identities = 87/198 (43%), Positives = 132/198 (66%), Gaps = 8/198 (4%)
 Frame = -1

Query: 572 LFFL-----PSSRKNLPPGPKPWPIIGNMLSMLTGAPHVELMKLARTHGPLMLVKFGAQP 408
           +FFL      S    LPPGPKPWPI+GN+   +   PH+ L   A+T+GPLM +K G Q 
Sbjct: 19  IFFLLKLCKSSKIPQLPPGPKPWPILGNI--HMGKMPHITLTNFAKTYGPLMSLKLGTQC 76

Query: 407 VIIASTPAAATEILKANDRILSGRFVPHSLRMKNHI--KHSVVWT-DCTDNWKTLRRIAR 237
           +++ S+P+ A EILK +DRILSGR VP++   K     K S+ WT +C + W+ LR + R
Sbjct: 77  LVVGSSPSVAIEILKTHDRILSGRHVPNAFPFKKSELEKSSIGWTSECNNGWRYLRTLCR 136

Query: 236 TELFSTKMLDAQLLVREKKVAEMVNFVRGQDGKAVVITEFVFGTLLNILGHVVFSKDVFQ 57
           TELFS K+L++Q  +REKKV E++ F+R ++G+ V + E +F T+LN+L +V+ S+D+ +
Sbjct: 137 TELFSGKVLESQACLREKKVKELIEFLRSKEGQVVNLGELIFATVLNMLSNVMISQDIVK 196

Query: 56  YGEKGDKVGMQRLIREML 3
             ++ +  GM+ LIRE +
Sbjct: 197 LEKETEDGGMKSLIREAM 214