BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cinnamomum23_contig00014956
         (672 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ...   115   9e-30
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   115   9e-30
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   115   9e-30
ref|XP_002513351.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   106   4e-29
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   104   9e-29
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   105   1e-27
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   110   2e-27
ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   100   4e-27
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   108   5e-27
ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   101   6e-27
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   108   8e-27
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   107   1e-26
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   106   2e-26
gb|KGN50421.1| hypothetical protein Csa_5G173480 [Cucumis sativus]    101   2e-26
emb|CBI31830.3| unnamed protein product [Vitis vinifera]               97   5e-26
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...    97   5e-26
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]    97   7e-26
ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   100   3e-25
gb|KDP34501.1| hypothetical protein JCGZ_11051 [Jatropha curcas]       97   3e-25
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   107   4e-25

>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
           californica [Expression vector pWCD2353]
          Length = 490

 Score =  115 bits (287), Expect(2) = 9e-30
 Identities = 55/106 (51%), Positives = 79/106 (74%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           AS+ S AS++LKTHDRILSARY+ Q+ R+K ++ +S+ +S DC+E WK LR +C+TELFT
Sbjct: 76  ASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWS-DCTETWKNLRKVCRTELFT 134

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            +M++ QAH+R+ K  E+V  L   +GE V + E++FGT  NI GN
Sbjct: 135 QKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 180



 Score = 42.7 bits (99), Expect(2) = 9e-30
 Identities = 27/77 (35%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDF 141
           EL  + N AD++P LG  D+ G  KE    +K IY IW                    DF
Sbjct: 209 ELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDF 268

Query: 140 VDVLQSAGCSEIQIKAL 90
           VDV   +G ++ QI AL
Sbjct: 269 VDVCLDSGLNDYQINAL 285


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
           AltName: Full=Cytochrome P450 80B2 [Eschscholzia
           californica] gi|3127029|gb|AAC39453.1|
           (S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
           californica]
          Length = 488

 Score =  115 bits (287), Expect(2) = 9e-30
 Identities = 55/106 (51%), Positives = 79/106 (74%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           AS+ S AS++LKTHDRILSARY+ Q+ R+K ++ +S+ +S DC+E WK LR +C+TELFT
Sbjct: 76  ASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWS-DCTETWKNLRKVCRTELFT 134

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            +M++ QAH+R+ K  E+V  L   +GE V + E++FGT  NI GN
Sbjct: 135 QKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 180



 Score = 42.7 bits (99), Expect(2) = 9e-30
 Identities = 27/77 (35%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDF 141
           EL  + N AD++P LG  D+ G  KE    +K IY IW                    DF
Sbjct: 209 ELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDF 268

Query: 140 VDVLQSAGCSEIQIKAL 90
           VDV   +G ++ QI AL
Sbjct: 269 VDVCLDSGLNDYQINAL 285


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1, partial
           [Eschscholzia californica] gi|3127027|gb|AAC39452.1|
           (S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
           californica]
          Length = 487

 Score =  115 bits (287), Expect(2) = 9e-30
 Identities = 55/106 (51%), Positives = 79/106 (74%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           AS+ S AS++LKTHDRILSARY+ Q+ R+K ++ +S+ +S DC+E WK LR +C+TELFT
Sbjct: 75  ASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWS-DCTETWKNLRKVCRTELFT 133

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            +M++ QAH+R+ K  E+V  L   +GE V + E++FGT  NI GN
Sbjct: 134 QKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 179



 Score = 42.7 bits (99), Expect(2) = 9e-30
 Identities = 27/77 (35%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDF 141
           EL  + N AD++P LG  D+ G  KE    +K IY IW                    DF
Sbjct: 208 ELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDF 267

Query: 140 VDVLQSAGCSEIQIKAL 90
           VDV   +G ++ QI AL
Sbjct: 268 VDVCLDSGLNDYQINAL 284


>ref|XP_002513351.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223547259|gb|EEF48754.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 318

 Score =  106 bits (265), Expect(2) = 4e-29
 Identities = 52/107 (48%), Positives = 78/107 (72%), Gaps = 2/107 (1%)
 Frame = -1

Query: 669 SSPSTASQVLKTHDRILSARYIPQTC--RIKFYISHSLPFSFDCSERWKTLRALCKTELF 496
           SSP  A+++LKTHDR+LSAR IP+    +I F    ++ ++  C+ERWK+LRALC+TELF
Sbjct: 92  SSPLAATEILKTHDRLLSARTIPKVLPYKIHFVDRLAIVWASSCNERWKSLRALCRTELF 151

Query: 495 TTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           +   ++ QA LR+ K++E+V  L S +G+VVN+GE+VF + FN + N
Sbjct: 152 SANAIESQAALREKKMIEMVEYLSSEQGQVVNIGEVVFTSVFNTISN 198



 Score = 48.9 bits (115), Expect(2) = 4e-29
 Identities = 26/75 (34%), Positives = 36/75 (48%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVD 135
           EL +APN+ADFYP   GLD QGL K+    +K+++  W                  DF+D
Sbjct: 226 ELTVAPNIADFYPVFAGLDPQGLQKKMLKYMKEMFSAWEIHIKERRETHVHGAPTNDFLD 285

Query: 134 VLQSAGCSEIQIKAL 90
           V  +    + QI  L
Sbjct: 286 VFLANDFDDDQINWL 300


>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223547255|gb|EEF48750.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 496

 Score =  104 bits (260), Expect(2) = 9e-29
 Identities = 49/108 (45%), Positives = 79/108 (73%), Gaps = 2/108 (1%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISH--SLPFSFDCSERWKTLRALCKTEL 499
           ASSP+ A+++LKTHDR+LS RYIP T   +  +    +L ++  CS++WK LRA+C++EL
Sbjct: 84  ASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSEL 143

Query: 498 FTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           F+ + ++ QA LR+ K+ E++  L S +G++VN+GE+VF T+FN + N
Sbjct: 144 FSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISN 191



 Score = 49.7 bits (117), Expect(2) = 9e-29
 Identities = 25/75 (33%), Positives = 37/75 (49%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVD 135
           EL   PN+ADFYP L  LD QG+ ++    +++++G+W                  DF+D
Sbjct: 219 ELATCPNIADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERRERHVKDARKTDFLD 278

Query: 134 VLQSAGCSEIQIKAL 90
           V  S G  + QI  L
Sbjct: 279 VFLSNGFDDHQINWL 293


>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Nelumbo nucifera]
          Length = 485

 Score =  105 bits (261), Expect(2) = 1e-27
 Identities = 52/105 (49%), Positives = 73/105 (69%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           ASS   A +VLK  DR+LS R+ P + RIK YI HS+ ++ DC++ WK +R + +TELF+
Sbjct: 76  ASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWA-DCTDYWKMVRKVWRTELFS 134

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILG 358
           T+MLD+QAH R+ KV EL+  L    GE VN  +++FG+  NILG
Sbjct: 135 TKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILG 179



 Score = 45.8 bits (107), Expect(2) = 1e-27
 Identities = 28/74 (37%), Positives = 35/74 (47%)
 Frame = -2

Query: 311 LVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVDV 132
           L   PNLAD YP LGG D QGL K +   VK++   W                  DF+ V
Sbjct: 210 LAAIPNLADLYPILGGSDFQGLRKASAACVKRMNESW--AAIVKQRRKNDDHSKNDFLQV 267

Query: 131 LQSAGCSEIQIKAL 90
           L  +G S+ QI A+
Sbjct: 268 LLDSGFSDPQIDAM 281


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  110 bits (276), Expect(2) = 2e-27
 Identities = 53/106 (50%), Positives = 79/106 (74%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           ASSP+ A++VLKTHD+ILS RY+ Q+ R+K ++ +S+ +S +C++ WK LR +C+TELFT
Sbjct: 76  ASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWS-ECNDNWKLLRKVCRTELFT 134

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            +M++ Q+ +R+ K  E+V  L S  GEVV + E+VFGT  NI GN
Sbjct: 135 PKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGN 180



 Score = 38.9 bits (89), Expect(2) = 2e-27
 Identities = 22/75 (29%), Positives = 37/75 (49%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVD 135
           E+  + N AD++P +G  D+ G  +    ++++IY +W                  DFVD
Sbjct: 209 EMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVW-GINLKERRGMKGSESNNDFVD 267

Query: 134 VLQSAGCSEIQIKAL 90
           VL +AG  + +I AL
Sbjct: 268 VLLNAGLEDQKINAL 282


>ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
           2-like [Solanum tuberosum]
          Length = 496

 Score =  100 bits (250), Expect(2) = 4e-27
 Identities = 53/107 (49%), Positives = 70/107 (65%), Gaps = 2/107 (1%)
 Frame = -1

Query: 669 SSPSTASQVLKTHDRILSARYIPQTCRIKF--YISHSLPFSFDCSERWKTLRALCKTELF 496
           SSPS A ++ KTHDRILS R++P   R K       SL +  +C   W+ LR LC+T+LF
Sbjct: 83  SSPSVAIEIFKTHDRILSGRHVPNAVRSKGSDLEKTSLGWKSECDSGWRYLRTLCRTDLF 142

Query: 495 TTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
             ++L+ QA LR+ KV+ELV  L S  G+VVN+GELVF T  N+L N
Sbjct: 143 AGKVLESQACLREKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSN 189



 Score = 48.1 bits (113), Expect(2) = 4e-27
 Identities = 21/38 (55%), Positives = 28/38 (73%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW 201
           E+  APN++DFYP LG LD+QGL K++T +V K   IW
Sbjct: 217 EVGSAPNISDFYPILGKLDLQGLRKKSTDLVTKFCSIW 254


>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  108 bits (269), Expect(2) = 5e-27
 Identities = 51/106 (48%), Positives = 78/106 (73%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           AS+P  AS++LKTHDR+LS RY+ Q+ R+K ++ +S+ +S +C+E WK LR +C+TELFT
Sbjct: 70  ASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWS-ECNETWKKLRKVCRTELFT 128

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            +M++ QA +R++K +E+V  L  + G  V + E+VFGT  NI GN
Sbjct: 129 QKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 174



 Score = 40.4 bits (93), Expect(2) = 5e-27
 Identities = 23/76 (30%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW-XXXXXXXXXXXXXXXXXRDFV 138
           EL  + N AD++P+LG  D+ G +K+    ++ IY +W                   DFV
Sbjct: 203 ELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDFV 262

Query: 137 DVLQSAGCSEIQIKAL 90
           ++L  +G  + QI AL
Sbjct: 263 EILLDSGLDDQQINAL 278


>ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
           2-like [Solanum tuberosum]
          Length = 495

 Score =  101 bits (252), Expect(2) = 6e-27
 Identities = 53/107 (49%), Positives = 72/107 (67%), Gaps = 2/107 (1%)
 Frame = -1

Query: 669 SSPSTASQVLKTHDRILSARYIPQTCRIKF--YISHSLPFSFDCSERWKTLRALCKTELF 496
           SS S A ++LKTHDRILS R++P     K       S+ ++ +C   W+ LR LCKTELF
Sbjct: 82  SSSSAAIEILKTHDRILSGRHVPNAMPAKRSDLDKISMGWNSECHNEWRCLRTLCKTELF 141

Query: 495 TTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           + ++L+ QA+LR+ KV+ELV  L S  G+VVN+GELVF T  N+L N
Sbjct: 142 SGKVLESQAYLREKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSN 188



 Score = 46.6 bits (109), Expect(2) = 6e-27
 Identities = 22/38 (57%), Positives = 26/38 (68%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW 201
           E V APNL+DFYP LG LD+QGL K A  ++ KI   W
Sbjct: 216 EAVSAPNLSDFYPVLGKLDLQGLRKRARYVMTKIRSNW 253


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
           isozyme 2; AltName: Full=Cytochrome P450 80B2 [Coptis
           japonica] gi|9971208|dbj|BAB12433.1|
           (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  108 bits (269), Expect(2) = 8e-27
 Identities = 51/106 (48%), Positives = 78/106 (73%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           ASSP+ A+++LKTHD+ILS RY+ Q+ R+K ++ +S+ +S +C++ WK LR +C+TELFT
Sbjct: 76  ASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWS-ECNDNWKLLRKVCRTELFT 134

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            +M++ Q+ +R+ K  E+V  L    GEVV + E+VFGT  NI GN
Sbjct: 135 PKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGN 180



 Score = 39.7 bits (91), Expect(2) = 8e-27
 Identities = 22/69 (31%), Positives = 35/69 (50%)
 Frame = -2

Query: 296 NLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVDVLQSAG 117
           N AD++P +G LD+ G  +    ++++IY +W                  DFVDVL +AG
Sbjct: 215 NPADYFPIMGKLDLFGQRRAVAEVLQQIYDVW-GVMLKERRGTKGSESKNDFVDVLLNAG 273

Query: 116 CSEIQIKAL 90
             + +I AL
Sbjct: 274 LDDQKINAL 282


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  107 bits (266), Expect(2) = 1e-26
 Identities = 50/106 (47%), Positives = 78/106 (73%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           AS+P  AS++LKTHDR+LS RY+ Q+ R+K ++ +S+ +S +C+E WK LR +C+T+LFT
Sbjct: 70  ASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWS-ECNETWKKLRKVCRTDLFT 128

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            +M++ QA +R++K +E+V  L  + G  V + E+VFGT  NI GN
Sbjct: 129 QKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 174



 Score = 40.4 bits (93), Expect(2) = 1e-26
 Identities = 23/76 (30%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW-XXXXXXXXXXXXXXXXXRDFV 138
           EL  + N AD++P+LG  D+ G +K+    ++ IY +W                   DFV
Sbjct: 203 ELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQHNNSKKNDFV 262

Query: 137 DVLQSAGCSEIQIKAL 90
           ++L  +G  + QI AL
Sbjct: 263 EILLDSGLDDQQINAL 278


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  106 bits (265), Expect(2) = 2e-26
 Identities = 50/106 (47%), Positives = 78/106 (73%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           AS+P  AS++LKTHDR+LS RY+ Q+ R+K ++ +S+ +S +C+E WK LR +C+ ELFT
Sbjct: 75  ASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWS-ECNETWKKLRKVCRAELFT 133

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            +M++ QA +R++K +E+V  L  ++G  V + E+VFGT  NI GN
Sbjct: 134 QKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGN 179



 Score = 40.0 bits (92), Expect(2) = 2e-26
 Identities = 23/76 (30%), Positives = 36/76 (47%), Gaps = 1/76 (1%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW-XXXXXXXXXXXXXXXXXRDFV 138
           EL  + N AD++P+LG  D+ G  K+    ++ IY +W                   DFV
Sbjct: 208 ELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLHNNSKKNDFV 267

Query: 137 DVLQSAGCSEIQIKAL 90
           ++L  +G  + QI AL
Sbjct: 268 EILLDSGLDDQQINAL 283


>gb|KGN50421.1| hypothetical protein Csa_5G173480 [Cucumis sativus]
          Length = 306

 Score =  101 bits (251), Expect(2) = 2e-26
 Identities = 55/107 (51%), Positives = 70/107 (65%), Gaps = 1/107 (0%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQ-TCRIKFYISHSLPFSFDCSERWKTLRALCKTELF 496
           ASSP+TA+ VLKTHDR LS RYI Q T     Y  HSL FS +C + WK LR++CK  LF
Sbjct: 84  ASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLF 143

Query: 495 TTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           T + ++ QA LR+ K+ ELV  L S +G VV + + VF + FN L N
Sbjct: 144 TAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSN 190



 Score = 45.1 bits (105), Expect(2) = 2e-26
 Identities = 27/76 (35%), Positives = 38/76 (50%), Gaps = 1/76 (1%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW-XXXXXXXXXXXXXXXXXRDFV 138
           +L + PNLADFYP L   D+QGL K+A   + ++  +W                   DF+
Sbjct: 217 DLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFL 276

Query: 137 DVLQSAGCSEIQIKAL 90
           DVL  +G S+ QI  L
Sbjct: 277 DVLIQSGFSDQQINYL 292


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score = 97.1 bits (240), Expect(2) = 5e-26
 Identities = 52/108 (48%), Positives = 72/108 (66%), Gaps = 2/108 (1%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKF-YISH-SLPFSFDCSERWKTLRALCKTEL 499
           ASSP+ A +VLKTHDR LS RY+  +  +K   ++H S+ F+ DC+  WK LRA+C+ EL
Sbjct: 625 ASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMEL 684

Query: 498 FTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           F+ + ++ Q  LR+ KV ELV  L +  GEVV + +LVF T  NIL N
Sbjct: 685 FSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSN 732



 Score = 48.1 bits (113), Expect(2) = 5e-26
 Identities = 25/72 (34%), Positives = 37/72 (51%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVD 135
           E    PNL+D+YP LGGLD+QG+N++A  + ++I   W                 RDF++
Sbjct: 761 EFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLE 820

Query: 134 VLQSAGCSEIQI 99
            L   G  + QI
Sbjct: 821 ALLEIGFEDDQI 832



 Score = 95.9 bits (237), Expect(2) = 4e-22
 Identities = 50/108 (46%), Positives = 72/108 (66%), Gaps = 2/108 (1%)
 Frame = -1

Query: 672  ASSPSTASQVLKTHDRILSARYIPQTCRIKF-YISH-SLPFSFDCSERWKTLRALCKTEL 499
            ASSP+ A +VLKTHDR LS RY+  +  +K   ++H SL F+ +C+  WK LR +C+TE+
Sbjct: 1130 ASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEM 1189

Query: 498  FTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            F+ + ++    LR+ KV+ELV  L +  GEVV + +LVF T  NIL N
Sbjct: 1190 FSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSN 1237



 Score = 36.2 bits (82), Expect(2) = 4e-22
 Identities = 16/38 (42%), Positives = 23/38 (60%)
 Frame = -2

Query: 314  ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW 201
            EL   PNL+DFYP L GL++ G  K++   + +I   W
Sbjct: 1261 ELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATW 1298



 Score = 84.0 bits (206), Expect(2) = 1e-21
 Identities = 45/108 (41%), Positives = 69/108 (63%), Gaps = 2/108 (1%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKF-YISH-SLPFSFDCSERWKTLRALCKTEL 499
           ASS + A +VLKTHDR LS RY+  T  +    ++H ++ F+  C+  W+ L+A+C+ EL
Sbjct: 85  ASSSAAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMEL 144

Query: 498 FTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           F+ + ++ +  LR+ KV+ELV  L    GEVV + +LV+ T  NIL N
Sbjct: 145 FSGKAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSN 192



 Score = 46.2 bits (108), Expect(2) = 1e-21
 Identities = 24/68 (35%), Positives = 32/68 (47%)
 Frame = -2

Query: 302 APNLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVDVLQS 123
           A N+ DFYP LGGLD+QG+ K+   I ++I   W                  DF+D L  
Sbjct: 224 ATNILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSSHGDFLDALLE 283

Query: 122 AGCSEIQI 99
            G  + QI
Sbjct: 284 TGFEDDQI 291


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 498

 Score = 97.1 bits (240), Expect(2) = 5e-26
 Identities = 52/108 (48%), Positives = 72/108 (66%), Gaps = 2/108 (1%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKF-YISH-SLPFSFDCSERWKTLRALCKTEL 499
           ASSP+ A +VLKTHDR LS RY+  +  +K   ++H S+ F+ DC+  WK LRA+C+ EL
Sbjct: 85  ASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMEL 144

Query: 498 FTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           F+ + ++ Q  LR+ KV ELV  L +  GEVV + +LVF T  NIL N
Sbjct: 145 FSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSN 192



 Score = 48.1 bits (113), Expect(2) = 5e-26
 Identities = 25/72 (34%), Positives = 37/72 (51%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVD 135
           E    PNL+D+YP LGGLD+QG+N++A  + ++I   W                 RDF++
Sbjct: 221 EFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLE 280

Query: 134 VLQSAGCSEIQI 99
            L   G  + QI
Sbjct: 281 ALLEIGFEDDQI 292


>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
          Length = 498

 Score = 96.7 bits (239), Expect(2) = 7e-26
 Identities = 52/108 (48%), Positives = 72/108 (66%), Gaps = 2/108 (1%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKF-YISH-SLPFSFDCSERWKTLRALCKTEL 499
           ASSP+ A +VLKTHDR LS RY+  +  +K   ++H S+ F+ DC+  WK LRA+C+ EL
Sbjct: 85  ASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMEL 144

Query: 498 FTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           F+ + ++ Q  LR+ KV ELV  L +  GEVV + +LVF T  NIL N
Sbjct: 145 FSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSN 192



 Score = 48.1 bits (113), Expect(2) = 7e-26
 Identities = 25/72 (34%), Positives = 37/72 (51%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVD 135
           E    PNL+D+YP LGGLD+QG+N++A  + ++I   W                 RDF++
Sbjct: 221 EFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLE 280

Query: 134 VLQSAGCSEIQI 99
            L   G  + QI
Sbjct: 281 ALLEIGFEDDQI 292


>ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           isoform X1 [Nicotiana sylvestris]
           gi|698520618|ref|XP_009757124.1| PREDICTED: probable
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 isoform
           X2 [Nicotiana sylvestris]
          Length = 489

 Score =  100 bits (248), Expect(2) = 3e-25
 Identities = 49/108 (45%), Positives = 72/108 (66%), Gaps = 2/108 (1%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYI--SHSLPFSFDCSERWKTLRALCKTEL 499
           ASSP +A+++LKT DR+LSAR +P+    K  +   HSL +S D S  WK+LRA C+T L
Sbjct: 77  ASSPDSAAEILKTQDRLLSARSVPRVAPYKLSVVDQHSLVWSSDLSNHWKSLRAFCRTHL 136

Query: 498 FTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           F+ + ++ QA LR+ KV E++  L S +G+ V + E++F T  N LGN
Sbjct: 137 FSAKAVESQAALREKKVFEMIEFLRSKKGKTVKISEIMFATILNTLGN 184



 Score = 42.4 bits (98), Expect(2) = 3e-25
 Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 2/77 (2%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDF 141
           EL   PN+++FYP+L  LD+QGL K+      +++ IW                   +DF
Sbjct: 212 ELGAMPNISEFYPFLDALDLQGLRKQTKIYQGQLFNIWAEIVKEKRQAISRGSSNKIQDF 271

Query: 140 VDVLQSAGCSEIQIKAL 90
           +D++  +G +++QI  +
Sbjct: 272 LDIMIYSGFTDLQINII 288


>gb|KDP34501.1| hypothetical protein JCGZ_11051 [Jatropha curcas]
          Length = 444

 Score = 97.1 bits (240), Expect(2) = 3e-25
 Identities = 49/108 (45%), Positives = 74/108 (68%), Gaps = 2/108 (1%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISH--SLPFSFDCSERWKTLRALCKTEL 499
           ASS   A ++LK +DR+LSAR +P+    K ++    SL ++  C+E+WK+ RALC+TEL
Sbjct: 81  ASSSKAAIEILKYNDRLLSARSVPKAVPRKSHVLERISLVWNPTCNEQWKSFRALCRTEL 140

Query: 498 FTTRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
           F+ + ++ +A LR+NK+ E+V  L S  GE VN+GE+VF T FN + N
Sbjct: 141 FSAKAVESRASLRENKMTEMVEYLSSKEGEEVNIGEVVFATVFNTISN 188



 Score = 45.4 bits (106), Expect(2) = 3e-25
 Identities = 27/77 (35%), Positives = 37/77 (48%), Gaps = 2/77 (2%)
 Frame = -2

Query: 314 ELVMAPNLADFYPWLGGLDVQGLNKEATGIVKKIYGIW--XXXXXXXXXXXXXXXXXRDF 141
           EL  APN+ADFYP    LD QG+ +  +  +K+++GIW                    DF
Sbjct: 212 ELGAAPNVADFYPTFAELDPQGIRRAISECLKEMFGIWEIYIKERRERHANAHNVFSTDF 271

Query: 140 VDVLQSAGCSEIQIKAL 90
           +DV  S G  + QI  L
Sbjct: 272 LDVFLSNGLDDDQINWL 288


>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  107 bits (267), Expect(2) = 4e-25
 Identities = 52/106 (49%), Positives = 75/106 (70%)
 Frame = -1

Query: 672 ASSPSTASQVLKTHDRILSARYIPQTCRIKFYISHSLPFSFDCSERWKTLRALCKTELFT 493
           AS+   A ++LKTHDRI SAR+I  + R+K +I +SL +S DC++ WK LR + +TE+F+
Sbjct: 79  ASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWS-DCTDYWKLLRKIVRTEIFS 137

Query: 492 TRMLDLQAHLRQNKVVELVGSLFSSRGEVVNLGELVFGTSFNILGN 355
            +ML  Q+H+R+ KV EL+  L S  G+VV + + VFGT  NILGN
Sbjct: 138 PKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGN 183



 Score = 34.7 bits (78), Expect(2) = 4e-25
 Identities = 22/70 (31%), Positives = 34/70 (48%)
 Frame = -2

Query: 299 PNLADFYPWLGGLDVQGLNKEATGIVKKIYGIWXXXXXXXXXXXXXXXXXRDFVDVLQSA 120
           PN+A+FYP L  LD+QGL K+      ++  +W                 +D +DVL + 
Sbjct: 216 PNVAEFYPSLEELDLQGLKKKCDERFIRVMKMW--EGTVKERKANRNEESKDMLDVLLAN 273

Query: 119 GCSEIQIKAL 90
             ++ QI AL
Sbjct: 274 DFNDAQINAL 283