BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cinnamomum23_contig00010462
         (1369 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   510   e-142
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...   500   e-139
ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran...   500   e-138
gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papa...   486   e-134
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        473   e-130
ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran...   464   e-128
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ...   455   e-125
gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyl...   452   e-124
ref|XP_010247902.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran...   447   e-123
ref|XP_010271691.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran...   434   e-119
gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353]     433   e-118
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth...   433   e-118
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt...   432   e-118
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap...   431   e-118
gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Pap...   428   e-117
gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papa...   414   e-113
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   409   e-111
gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353]    408   e-111
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   408   e-111
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc...   406   e-110

>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT [Coptis japonica]
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  510 bits (1314), Expect = e-142
 Identities = 243/347 (70%), Positives = 294/347 (84%)
 Frame = -3

Query: 1289 MEVQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSV 1110
            MEV+K + ++QAK+W  IYGF+ESLVL+CAV+L +A+IIH  G ++++ +L+S++P   V
Sbjct: 1    MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPV 60

Query: 1109 NVDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMA 930
            N D LYR+MR+LVHMKLF+  S+ GELRYGL P A+ LVKGWDKCMV SIL+ITDKDFMA
Sbjct: 61   NEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMA 120

Query: 929  PWHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKE 750
            PWHY+K+ L  ES TAFEK LG NIW YMA++P+KNQLFNE MA DSRL+ SAL+++C  
Sbjct: 121  PWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 180

Query: 749  MFEGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
            +F GI +LVDVGGGTGTA+R IA AFPH+KCTVYDLPHVIADSP Y+EV  V GDMFK I
Sbjct: 181  IFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI 240

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            P ADAIMMKCILHDWDD+ECI+ILK+C+EAVP  GGKVIIVDIVL+V+S+  Y+ MR  +
Sbjct: 241  PKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTL 300

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            DLDMMLNTGGKERTEEEWKKLI+DAG+ G+KI  I+AVQSVIEAYPY
Sbjct: 301  DLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYPY 347


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
            glaucum]
          Length = 350

 Score =  500 bits (1288), Expect = e-139
 Identities = 233/347 (67%), Positives = 293/347 (84%), Gaps = 2/347 (0%)
 Frame = -3

Query: 1283 VQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSVNV 1104
            + K + ++QAK+W  IYGF++SLVL+ AV+L +A+IIH HG  +++ +L+  +P   VN 
Sbjct: 4    INKENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQ 63

Query: 1103 DHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAPW 924
            D LYR++R+LVHMKLF+  S+ GELRYGL P A+ LVKGWDKCM+ +IL+ITDKDFMAPW
Sbjct: 64   DALYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPW 123

Query: 923  HYIKESLGKE--SPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKE 750
            HY+KE +  +  + TAFEK LG NIWDYMA++P+KNQLFNEGMA D+RL+ SAL+++C  
Sbjct: 124  HYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSS 183

Query: 749  MFEGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
            MF+GI ++VDVGGGTGTA+R IAKAFPH+KCTVYDLPHVIADSP Y E+  + GDMFK I
Sbjct: 184  MFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKYI 243

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            P+ADAIMMKCILHDWDD+ECI+ILK+C++AVP +GGKVII+DI+LDV+S+  Y+ MR  +
Sbjct: 244  PNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMRLTL 303

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            DLDMMLNTGGKERTEEEWKKLI+DAG+ GYKI  ISAVQSVIEAYPY
Sbjct: 304  DLDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYPY 350


>ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
            nucifera]
          Length = 346

 Score =  500 bits (1287), Expect = e-138
 Identities = 235/347 (67%), Positives = 292/347 (84%)
 Frame = -3

Query: 1289 MEVQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSV 1110
            ME+QK  QAA AKIW+ +YGF++ LVLRCA++LGIADIIH+ G+ +++ +L ++IPV  V
Sbjct: 1    MEIQKEGQAAAAKIWKFVYGFADCLVLRCAIDLGIADIIHKQGEPLTLSELGAQIPVQPV 60

Query: 1109 NVDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMA 930
            N DHL+R+MR+LVHMK+F+ +++ GE RYGL P A+ +VKGWDK +V  IL +TDKDFMA
Sbjct: 61   NTDHLHRLMRYLVHMKIFTKETLDGEARYGLAPPAKFIVKGWDKSIVSIILVVTDKDFMA 120

Query: 929  PWHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKE 750
            PWH +K+SL  E  TAFEK LG +IW YMAD+P+KN+LFNEGMACD++LL SAL+QDCK+
Sbjct: 121  PWHCLKDSLCGEG-TAFEKALGRSIWTYMADHPEKNKLFNEGMACDTKLLISALVQDCKD 179

Query: 749  MFEGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
            +F+GI+SLVDVGGGTGTA+RAIAKAFPH+KCT+YDLPHVIADSPDY EV R+ GDMFK I
Sbjct: 180  LFQGIMSLVDVGGGTGTAMRAIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMFKHI 239

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            PSADAI++KCILHDWDD ECI+ILK+C+E+VP  GGKVIIVDIV+D+ES    +  R  +
Sbjct: 240  PSADAILLKCILHDWDDGECIEILKRCKESVPREGGKVIIVDIVVDLESKHPLTKTRLSL 299

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            DLDMM+ TGGKERTE EWKKL+  AGF  +KI  ISAVQSVI AYPY
Sbjct: 300  DLDMMVTTGGKERTEAEWKKLLNAAGFPVFKITHISAVQSVIVAYPY 346


>gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
          Length = 348

 Score =  486 bits (1252), Expect = e-134
 Identities = 230/349 (65%), Positives = 288/349 (82%), Gaps = 2/349 (0%)
 Frame = -3

Query: 1289 MEVQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSV 1110
            ME  K DQA QA IW+LIYGF+ESLVL+CA++L IAD IH HG+ +S+ +LASK+PV  V
Sbjct: 1    MEATKSDQANQANIWKLIYGFAESLVLKCAIQLEIADTIHNHGEPMSLSELASKLPVQPV 60

Query: 1109 NVDHLYRIMRFLVHMKLFSIQ--SVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDF 936
            N D LYR+MR+LVHMKLF+ +  S+ GE +Y L P A+ L+KGW+K MV SIL+I DKDF
Sbjct: 61   NSDRLYRVMRYLVHMKLFNKEKTSINGEFKYSLAPPAKFLIKGWEKSMVASILAINDKDF 120

Query: 935  MAPWHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDC 756
            +APWH++K+ L  +   AFEK LG +IW YM++NP+KNQLFNE MACD+RL+TSAL+ DC
Sbjct: 121  LAPWHHLKDGLSGDCD-AFEKALGKSIWVYMSENPEKNQLFNEAMACDTRLVTSALVNDC 179

Query: 755  KEMFEGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFK 576
            + +F+GI +LVDVGGGTGTA++AI+KAFPH+KC++YDLPHVIADSP+   V ++ GDMFK
Sbjct: 180  QSVFKGINTLVDVGGGTGTAVKAISKAFPHIKCSIYDLPHVIADSPEIPNVVKIEGDMFK 239

Query: 575  SIPSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRR 396
            +IPSADAI+MKCILHDW+D ECI+ILKKC+EAVP  GGKVIIVD+VL+++    YS +R 
Sbjct: 240  AIPSADAILMKCILHDWNDDECIQILKKCKEAVPQEGGKVIIVDVVLNMDLTHPYSKIRL 299

Query: 395  CMDLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
             +DLDMMLNTGGKERT EEWKKLI  AGF  +KI  ISAVQSVIEA+PY
Sbjct: 300  TLDLDMMLNTGGKERTVEEWKKLIDAAGFASFKITEISAVQSVIEAFPY 348


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  473 bits (1217), Expect = e-130
 Identities = 226/345 (65%), Positives = 282/345 (81%), Gaps = 2/345 (0%)
 Frame = -3

Query: 1277 KGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSVNVDH 1098
            K DQA QAKIW+ IYGF+ESL+L+ AVEL IAD IH HG  +++ +LASK+PV  V++D 
Sbjct: 8    KIDQATQAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDR 67

Query: 1097 LYRIMRFLVHMKLFSIQSVKGE--LRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAPW 924
            LYR+MR+LVHMKLF  Q  + E  ++Y L P A+ L+KGW+K MV +IL I DKDFM+PW
Sbjct: 68   LYRVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPW 127

Query: 923  HYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEMF 744
            H++KE L   S  AF+K LG +IWDYM+ NP KN++FNEGMACDSRL++SAL+ +C  +F
Sbjct: 128  HHLKEGLVNNS-NAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNECGNIF 186

Query: 743  EGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSIPS 564
            +GI +LVDVGGGTGTA++A++KAFPH+KC++YDLPHVI+DSPD   + ++ G+MFKSIPS
Sbjct: 187  QGIQTLVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKSIPS 246

Query: 563  ADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCMDL 384
            ADAI+MKCILHDW+D ECI+ILKKC+EAVP  GGKVIIVDIVLD+ES   YS +R  +DL
Sbjct: 247  ADAILMKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSKVRLTLDL 306

Query: 383  DMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            DMMLNT GKERTEEEWKKLI  AGF  YKI  ISA+QSVIEA+PY
Sbjct: 307  DMMLNTEGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAFPY 351


>ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
            nucifera]
          Length = 346

 Score =  464 bits (1193), Expect = e-128
 Identities = 220/347 (63%), Positives = 279/347 (80%)
 Frame = -3

Query: 1289 MEVQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSV 1110
            ME+ K  QA + +IW+  Y F+++LVLRCA+E GIADIIH+ G+ +++ +L ++IPV  V
Sbjct: 1    MEIPKEVQADEVEIWKFGYDFADTLVLRCAIEFGIADIIHKQGEPLTLFELGAQIPVQPV 60

Query: 1109 NVDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMA 930
            N DHL+R+MR++VHMK+F+ +++ GE  YGL P  + LVKGWDK M  +IL+ITD+DF A
Sbjct: 61   NTDHLHRLMRYMVHMKIFTKETLGGEEXYGLSPHGKFLVKGWDKSMASAILAITDEDFFA 120

Query: 929  PWHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKE 750
            PWH +K+ L  E  TAFEK LG +IW Y+AD+P+KN+LFNE MACD+  +TS LIQDCK+
Sbjct: 121  PWHCLKDVLAGEG-TAFEKALGKSIWAYVADHPEKNKLFNEVMACDTSFITSVLIQDCKD 179

Query: 749  MFEGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
            +F+GI S+VDVGGGTGTA+R IAKAFPH+KCT+YDLPHVIADSPDY EV R+ GDMFK I
Sbjct: 180  VFQGIKSVVDVGGGTGTAMRDIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMFKHI 239

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            PSADAI++K ILHDWDD ECI+ILK+C+E+VP  GGKVIIVDIVLD ES    +  R  +
Sbjct: 240  PSADAILLKWILHDWDDGECIEILKRCKESVPREGGKVIIVDIVLDPESKDPLTKARLRL 299

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            DLDMM+ TGGKER+E EWKKL+  AGF GYKI  ++AVQSVI AYPY
Sbjct: 300  DLDMMVYTGGKERSEAEWKKLLNAAGFPGYKILHVAAVQSVIMAYPY 346


>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum flavum]
          Length = 346

 Score =  455 bits (1171), Expect = e-125
 Identities = 217/344 (63%), Positives = 280/344 (81%), Gaps = 3/344 (0%)
 Frame = -3

Query: 1271 DQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSVNVDHLY 1092
            D ++QAK+W  IYGF++SLVL+CAV+L +A+IIH+HG  +++ +L+S++P   VN D LY
Sbjct: 3    DLSSQAKLWNYIYGFADSLVLKCAVQLDLANIIHDHGTPMTLSELSSRLPSQPVNQDALY 62

Query: 1091 RIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAPWHYIK 912
            R+MR+LVH+KLF+  S+ GELRYGL P A+ LVKGWD CM+ SIL + D DFMAP+HYIK
Sbjct: 63   RVMRYLVHIKLFTKSSIDGELRYGLAPPAKFLVKGWDNCMIGSILLMVDTDFMAPYHYIK 122

Query: 911  ESLGKE--SPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEMFEG 738
            + +  +  S TAFEK  G NIW+Y+A++P+KNQLFNEGMA +SRL  S L++DC   FEG
Sbjct: 123  DEILNDGSSITAFEKASGTNIWEYLAEHPEKNQLFNEGMANESRLTMSGLMKDCSSTFEG 182

Query: 737  ILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSIPSAD 558
            + +LVDVGGGTGT ++ IAKAFP++K TVYDLPHVIAD+P   E+  V GDMFK IP+AD
Sbjct: 183  LTTLVDVGGGTGTTVKYIAKAFPNIKYTVYDLPHVIADAPGDTEINFVEGDMFKYIPNAD 242

Query: 557  AIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCMDLDM 378
            AI+MK +LHDWDD ECI+ILK+C++AVP +GGKVII+D+VL+V+S+  Y+ MR  +DLDM
Sbjct: 243  AILMKGVLHDWDDNECIEILKRCKDAVPRDGGKVIIIDVVLNVKSEHPYTKMRLTLDLDM 302

Query: 377  MLNTGGKERTEEEWKKLIY-DAGFTGYKIKSISAVQSVIEAYPY 249
            MLN  GKERTEE+WKKLIY DAG+ GYKI  ISA+QSVIEAYPY
Sbjct: 303  MLNNKGKERTEEDWKKLIYDDAGYKGYKITQISALQSVIEAYPY 346


>gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyllum hexandrum]
          Length = 350

 Score =  452 bits (1162), Expect = e-124
 Identities = 218/350 (62%), Positives = 275/350 (78%), Gaps = 3/350 (0%)
 Frame = -3

Query: 1289 MEVQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPV-NS 1113
            ME QK + ++QAK+W  IYGF+ESLVL+CAVEL  A+IIH HG  +++ +LAS++PV   
Sbjct: 1    MEAQKENISSQAKLWNFIYGFAESLVLKCAVELDFANIIHNHGKPMTLSELASQLPVMQP 60

Query: 1112 VNVDHLYRIMRFLVHMKLFS--IQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKD 939
            VN + LYR+MR+LVH+ +F+  +    GE +YGL   A+ LVKGWD CMV SIL ITDK 
Sbjct: 61   VNTNSLYRVMRYLVHINIFTKTLDENDGETKYGLAAPAKFLVKGWDNCMVGSILGITDKV 120

Query: 938  FMAPWHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQD 759
            FM PW+YIK+ L   + TAFE  LG +IW+YM +NP+KN+LFN  MACD+ ++ SALI +
Sbjct: 121  FMEPWYYIKDELAPGTGTAFELALGKDIWEYMGENPEKNKLFNAAMACDTSMIMSALISE 180

Query: 758  CKEMFEGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMF 579
            CK+ F GI +LVDVGGGTGTA R IA+AFP++KCTVYDLPHVIADSP Y E+ RV GDMF
Sbjct: 181  CKDKFNGIRTLVDVGGGTGTAARNIARAFPNIKCTVYDLPHVIADSPVYPEINRVSGDMF 240

Query: 578  KSIPSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMR 399
            K IP+ADAI+MKCILHDW+D+ECI+ILK+C+EAVP +GGKVII+D+VLD ES+  Y+ +R
Sbjct: 241  KCIPNADAILMKCILHDWEDKECIEILKRCKEAVPVDGGKVIIIDVVLDGESEHPYTKVR 300

Query: 398  RCMDLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
               DLDMMLNT GKERT+E WKKL   AG+  Y I  ISA+QSVIEA+PY
Sbjct: 301  LNSDLDMMLNTEGKERTKEGWKKLFKAAGYRDYNITQISALQSVIEAFPY 350


>ref|XP_010247902.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
            nucifera]
          Length = 347

 Score =  447 bits (1151), Expect = e-123
 Identities = 218/348 (62%), Positives = 279/348 (80%), Gaps = 1/348 (0%)
 Frame = -3

Query: 1289 MEVQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSV 1110
            ME+QK  QAA  +I +  YGF++ LV+RCA++LGIADIIH+ G+ +++ +L ++IPV  V
Sbjct: 1    MEIQKEVQAADVEIRKFGYGFADILVIRCAIQLGIADIIHKQGEPLTLSELEAQIPVKPV 60

Query: 1109 NVDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMA 930
            N DHL+R+MR++VHMK+F+ ++  GE RYGL P  + LV GWD+ MV +IL++TDKDFM 
Sbjct: 61   NTDHLHRLMRYMVHMKIFTKETPDGEERYGLAPLGKFLVNGWDRNMVSAILAVTDKDFMV 120

Query: 929  PWHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDS-RLLTSALIQDCK 753
            PW+ +K+SL  E  TAFEK LG  I + MAD+P+K + FNE MACD+ RLLTSALIQDCK
Sbjct: 121  PWYRLKDSLVGEG-TAFEKALGKTICECMADHPEKKKPFNEAMACDTTRLLTSALIQDCK 179

Query: 752  EMFEGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKS 573
            ++F+GI+SLVDVGGGTGTA+R IAK FPH+KCT+YDLPHVIADSPDY EV R+ G+MFK 
Sbjct: 180  DLFQGIMSLVDVGGGTGTAMRDIAKTFPHLKCTIYDLPHVIADSPDYPEVDRIAGNMFKH 239

Query: 572  IPSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRC 393
            IPSAD I++KCILHD  D++CI+IL++C+E+VP  GGKVIIVDIVLD ES    +  R  
Sbjct: 240  IPSADGILLKCILHDLGDRQCIEILQRCKESVPREGGKVIIVDIVLDPESTDPLTKARLR 299

Query: 392  MDLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            +DLDMM+ TGGKER+E EWKKL+  AGF  YKI  I+AVQSVIEAYPY
Sbjct: 300  LDLDMMVYTGGKERSEAEWKKLLNAAGFPRYKILHIAAVQSVIEAYPY 347


>ref|XP_010271691.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
            nucifera]
          Length = 351

 Score =  434 bits (1117), Expect = e-119
 Identities = 218/351 (62%), Positives = 272/351 (77%), Gaps = 4/351 (1%)
 Frame = -3

Query: 1289 MEVQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSV 1110
            ME QK  QAA+AKIW  +YGF+++LVLRCA+ELGIADIIH+ G+ +++ +L ++IP+ SV
Sbjct: 1    MENQKEVQAAEAKIWNFVYGFADTLVLRCAIELGIADIIHKQGEPLTLSELGAQIPLKSV 60

Query: 1109 NVDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGW-DKCMVPSILSITDKDFM 933
            N DHL+R+MR+LVHMKLF+ +++ GE RYGL P A+ LVK W DK +   I  ITDKDF+
Sbjct: 61   NTDHLHRLMRYLVHMKLFTKETLDGEARYGLAPPAKLLVKWWEDKGLASIIFGITDKDFI 120

Query: 932  APWHYIKESL-GKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDC 756
            APWH++K+SL G    T FEK LG +I  YMAD+ +K+ LFNE M  D+RL TS LIQD 
Sbjct: 121  APWHHLKDSLAGDGEETTFEKVLGKSISTYMADHLEKSMLFNESMVHDTRLFTSVLIQDF 180

Query: 755  KEMFEGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFK 576
            K++F+GI SLVDVGGG+GT + AIAKAFPH+KCT+Y LPHVIADSPDY EV R+ GDMFK
Sbjct: 181  KDVFQGIKSLVDVGGGSGTDMGAIAKAFPHLKCTIYGLPHVIADSPDYPEVDRISGDMFK 240

Query: 575  SIPSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHY-SNMR 399
             IPSADAI++KCILH W D +CI+ILK+C+E+VP  GG VII D V+D+ES   Y +   
Sbjct: 241  HIPSADAILLKCILHYWGDGQCIEILKRCKESVPREGGIVIIADAVVDLESKHPYLTKTL 300

Query: 398  RCMDLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQ-SVIEAYPY 249
               DLDMMLNTGGKERTE EWKKL   AGF  YKI  ++ V+ SVIEAYPY
Sbjct: 301  LSTDLDMMLNTGGKERTEAEWKKLFNAAGFPAYKITHVADVEYSVIEAYPY 351


>gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353]
          Length = 348

 Score =  433 bits (1114), Expect = e-118
 Identities = 215/347 (61%), Positives = 272/347 (78%), Gaps = 2/347 (0%)
 Frame = -3

Query: 1283 VQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPV-NSVN 1107
            V K DQ  QAKIW+ IYGF+ESLVL+CAV+L IA+ +H +   +S+ +LASK+PV   VN
Sbjct: 4    VSKIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVN 63

Query: 1106 VDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAP 927
             D L+RIMR+LVHM+LF I +     +Y L P A+ L++GW+K MV SIL I DKDF+AP
Sbjct: 64   EDRLFRIMRYLVHMELFKIDATTQ--KYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 121

Query: 926  WHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEM 747
            WH++ + L      AFEK LG +IW YM+ NP+KNQLFN  MACD+RL+TSAL  +CK +
Sbjct: 122  WHHLGDGLTGNCD-AFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSI 180

Query: 746  F-EGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
            F +GI +LVDVGGGTGTA++AI+KAFP +KCT+YDLPHVIADSP+   + ++ GDMFKSI
Sbjct: 181  FSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            PSADAI MKCILHDW+D ECI+ILK+C+EA+ P GGKVIIVD+V+D++S   Y+ +R  +
Sbjct: 241  PSADAIFMKCILHDWNDDECIQILKRCKEAL-PKGGKVIIVDVVIDMDSTHPYAKIRLTL 299

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            DLDMMLNTGGKERT+EEWK L   AGF  +K+  ISAVQSVIEAYPY
Sbjct: 300  DLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYPY 346


>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; Short=6OMT
            [Papaver somniferum] gi|33286374|gb|AAQ01669.1|
            (R,S)-norcoclaurine 6-O-methyltransferase [Papaver
            somniferum] gi|571330878|gb|AHF27395.1|
            (R,S)-norcoclaurine 6-O-methyltransferase [synthetic
            construct]
          Length = 346

 Score =  433 bits (1114), Expect = e-118
 Identities = 215/347 (61%), Positives = 272/347 (78%), Gaps = 2/347 (0%)
 Frame = -3

Query: 1283 VQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPV-NSVN 1107
            V K DQ  QAKIW+ IYGF+ESLVL+CAV+L IA+ +H +   +S+ +LASK+PV   VN
Sbjct: 4    VSKIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVN 63

Query: 1106 VDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAP 927
             D L+RIMR+LVHM+LF I +     +Y L P A+ L++GW+K MV SIL I DKDF+AP
Sbjct: 64   EDRLFRIMRYLVHMELFKIDATTQ--KYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 121

Query: 926  WHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEM 747
            WH++ + L      AFEK LG +IW YM+ NP+KNQLFN  MACD+RL+TSAL  +CK +
Sbjct: 122  WHHLGDGLTGNCD-AFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSI 180

Query: 746  F-EGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
            F +GI +LVDVGGGTGTA++AI+KAFP +KCT+YDLPHVIADSP+   + ++ GDMFKSI
Sbjct: 181  FSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            PSADAI MKCILHDW+D ECI+ILK+C+EA+ P GGKVIIVD+V+D++S   Y+ +R  +
Sbjct: 241  PSADAIFMKCILHDWNDDECIQILKRCKEAL-PKGGKVIIVDVVIDMDSTHPYAKIRLTL 299

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            DLDMMLNTGGKERT+EEWK L   AGF  +K+  ISAVQSVIEAYPY
Sbjct: 300  DLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYPY 346


>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase [Papaver
            somniferum]
          Length = 346

 Score =  432 bits (1110), Expect = e-118
 Identities = 214/347 (61%), Positives = 272/347 (78%), Gaps = 2/347 (0%)
 Frame = -3

Query: 1283 VQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPV-NSVN 1107
            V K DQ  QAKIW+ IYGF+ESLVL+CAV+L IA+ +H +   +S+ +LASK+PV   VN
Sbjct: 4    VSKIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVN 63

Query: 1106 VDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAP 927
             D L+RIMR+LVHM+LF I +     +Y L P A+ L++GW+K MV SIL I DKDF+AP
Sbjct: 64   EDRLFRIMRYLVHMELFKIDATTQ--KYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 121

Query: 926  WHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEM 747
            WH++ + L      AFEK LG +IW YM++NP+KNQLFN  MACD+RL+TSAL  +CK +
Sbjct: 122  WHHLGDGLTGNCD-AFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI 180

Query: 746  F-EGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
            F +GI +LVDVGGGTGTA++AI+KAFP +KCT+YDLPHVIADSP+   + ++ GDMFKSI
Sbjct: 181  FSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            PSADAI MKCILHDW+D ECI+ILK+C+EA+ P  GKVIIVD+V+D++S   Y+ +R  +
Sbjct: 241  PSADAIFMKCILHDWNDDECIQILKRCKEAL-PKVGKVIIVDVVIDMDSTHPYAKIRLTL 299

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            DLDMMLNTGGKERT+EEWK L   AGF  +K+  ISAVQSVIEAYPY
Sbjct: 300  DLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYPY 346


>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 346

 Score =  431 bits (1109), Expect = e-118
 Identities = 213/347 (61%), Positives = 273/347 (78%), Gaps = 2/347 (0%)
 Frame = -3

Query: 1283 VQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPV-NSVN 1107
            V K DQ  QAKIW+ IYGF+ESLVL+CAV+L IA+ +H +   +S+ +LASK+PV   VN
Sbjct: 4    VSKIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVPQPVN 63

Query: 1106 VDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAP 927
             D L+RIMR+LVHM+LF I +     +Y L P A+ L++GW+K MV SIL I DKDF+AP
Sbjct: 64   EDRLFRIMRYLVHMELFKIDATTQ--KYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 121

Query: 926  WHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEM 747
            WH++ + L      AFEK LG +IW YM++NP+KNQLFN  MACD+RL+TSAL  +CK +
Sbjct: 122  WHHLGDGLTGNCD-AFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI 180

Query: 746  F-EGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
            F +GI +LVDVGGGTGTA++ I+KAFP +KCT+YDLPHVIADSP+   + ++ GDMFKSI
Sbjct: 181  FSDGIKTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            PSADAI MKCILHDW+D+ECI+ILK+C+EA+P + GKVIIVD+V+D++S   Y+ +R  +
Sbjct: 241  PSADAIFMKCILHDWNDEECIQILKRCKEALPKD-GKVIIVDVVIDMDSTHPYAKIRLTL 299

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYPY 249
            DLDMMLNTGGKERT+EEWK L   AGF  +K+  ISAVQSVIEAYPY
Sbjct: 300  DLDMMLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYPY 346


>gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Papaver
            pseudo-orientale]
          Length = 343

 Score =  428 bits (1101), Expect = e-117
 Identities = 212/346 (61%), Positives = 271/346 (78%), Gaps = 2/346 (0%)
 Frame = -3

Query: 1283 VQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPV-NSVN 1107
            V K DQ  QAKIW+ IYGF+ESLVL+CAV+L IA+ +H +   +S+ +LASK+PV   VN
Sbjct: 2    VSKIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVN 61

Query: 1106 VDHLYRIMRFLVHMKLFSIQSVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAP 927
             D L+RIMR+LVHM+LF I +     +Y L P A+ L++GW+K MV SIL I DKDF+AP
Sbjct: 62   EDRLFRIMRYLVHMELFKIDATTQ--KYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 119

Query: 926  WHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEM 747
            WH++ + L      AFEK LG +IW YM++NP+KNQLFN  MACD+RL+TSAL  +CK +
Sbjct: 120  WHHLGDGLTGNCD-AFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI 178

Query: 746  F-EGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
            F +GI +LVDVGGGTGTA++ I+KAFP +KCT+YDLPHVIADSP+   + ++ GDMFKSI
Sbjct: 179  FSDGIRTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 238

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            PSADAI MKCILHDW D+ECI+ILK+C+EA+P + GKVIIVD+V+D++S   Y+ +R  +
Sbjct: 239  PSADAIFMKCILHDWSDEECIQILKRCKEALPKD-GKVIIVDVVIDMDSTHPYAKIRLTL 297

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYP 252
            DLDMMLNTGGKERT+EEWK L   AGF  +K+  ISAVQSVIEAYP
Sbjct: 298  DLDMMLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYP 343


>gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
          Length = 353

 Score =  414 bits (1065), Expect = e-113
 Identities = 193/346 (55%), Positives = 267/346 (77%), Gaps = 1/346 (0%)
 Frame = -3

Query: 1286 EVQKGDQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSVN 1107
            E Q+ D  AQA +W +IYGF++SLVLRCAVE+GIADII  +  +IS+ +LASK+P+ +VN
Sbjct: 9    ESQEVDIKAQAHLWNIIYGFADSLVLRCAVEIGIADIIKSNNGSISVTELASKLPITNVN 68

Query: 1106 VDHLYRIMRFLVHMKLFSIQSVKGELR-YGLEPAARCLVKGWDKCMVPSILSITDKDFMA 930
             D+LYR++R+LVHM +    S   E++ Y L+P A  L++  ++ MVP IL +T KDFM 
Sbjct: 69   SDNLYRVLRYLVHMGILKEVSDSNEVKLYSLQPVATLLLRDAERSMVPIILGMTQKDFMI 128

Query: 929  PWHYIKESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKE 750
            PWH++KE LG ++ TAFEKG+GM IW Y+  +P+++ LFNEGMA ++RLLT +LI  C++
Sbjct: 129  PWHFMKEGLGNDT-TAFEKGMGMTIWQYLEGHPEQSNLFNEGMAGETRLLTKSLIDGCRD 187

Query: 749  MFEGILSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSI 570
             FEG+ SL DVGGG GT ++ I  AFP +KC+VYDLPHVIA SP++  + R+PGDMFKS+
Sbjct: 188  TFEGLTSLCDVGGGNGTTIKGIYDAFPQIKCSVYDLPHVIASSPEHPNIERIPGDMFKSV 247

Query: 569  PSADAIMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCM 390
            PSA AI++K ILHDW D+EC+ IL KCREAVP + GKVIIVD+ L+ ES    +  R  +
Sbjct: 248  PSAQAILLKLILHDWTDEECVNILIKCREAVPKDTGKVIIVDVALEEESQHELTKTRLIL 307

Query: 389  DLDMMLNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYP 252
            D+DM++NTGG+ER+E++W+KL+  AGF G+KI+ I+A+QSVIEA+P
Sbjct: 308  DIDMLVNTGGRERSEDDWEKLLKRAGFRGHKIRHIAAIQSVIEAFP 353


>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  409 bits (1051), Expect = e-111
 Identities = 194/341 (56%), Positives = 267/341 (78%), Gaps = 1/341 (0%)
 Frame = -3

Query: 1271 DQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSVNVDHLY 1092
            D  AQA +W++IYGF++SLVLRCAVELGI DI+  +   +++  LASK+PV++VN D+LY
Sbjct: 11   DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLPVSNVNCDNLY 70

Query: 1091 RIMRFLVHMKLFSIQ-SVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAPWHYI 915
            RI+R+LV M++  ++ S  GE +Y LEP A  L +   + MVP IL +T KDFM PWH +
Sbjct: 71   RILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHSM 130

Query: 914  KESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEMFEGI 735
            K+ L  ++ TAFEK +GM IW+Y+  +P ++QLFNEGMA ++RLLTS+LI   ++MF+GI
Sbjct: 131  KDGLS-DNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQGI 189

Query: 734  LSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSIPSADA 555
             SLVDVGGG GT ++AI+ AFPH+KCT++DLPHVIA+S D+  + R+ GDMFKS+PSA A
Sbjct: 190  DSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKSVPSAQA 249

Query: 554  IMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCMDLDMM 375
            I++K ILHDW+D++ IKILK+CR AVP +GGKVIIVD+ LD ESD   S+ R  +D+DM+
Sbjct: 250  ILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDIDML 309

Query: 374  LNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYP 252
            +NTGGKERT+E W+K++  AGF+G KI+ I+A+QSVIE +P
Sbjct: 310  VNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350


>gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353]
          Length = 359

 Score =  408 bits (1048), Expect = e-111
 Identities = 185/338 (54%), Positives = 261/338 (77%), Gaps = 2/338 (0%)
 Frame = -3

Query: 1259 QAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSVNVDHLYRIMR 1080
            QA++W +IYGF++SLVLRCAVE+GIADII  +   I++ QLA+K+P+ +V+ D+LYR++R
Sbjct: 20   QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVR 79

Query: 1079 FLVHMKLFSIQSVKG--ELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAPWHYIKES 906
            +LVH+ +   ++  G  E  Y L+P    L++  ++ MVP IL +T KDFM  WH++KE 
Sbjct: 80   YLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEG 139

Query: 905  LGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEMFEGILSL 726
            LG  S TAFEKG+GM+IW Y+  NP ++QLFNEGMA ++RLLT  LI+DC++ F+G+ SL
Sbjct: 140  LGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGLDSL 199

Query: 725  VDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSIPSADAIMM 546
            VD+GGG GT ++AI +AFPH+KCT+YDLPHV+A+S D   + +VPGDMFKS+PSA AI++
Sbjct: 200  VDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAILL 259

Query: 545  KCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCMDLDMMLNT 366
            K ILHDW D+EC+ ILKKC+EA+P   GKVIIVD+ L+ ES+   +  R  +D+DM++NT
Sbjct: 260  KLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVNT 319

Query: 365  GGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYP 252
            GG+ERT ++W+ L+  AGF  +KI+ I A+QSVIEA+P
Sbjct: 320  GGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase 2 [Papaver somniferum]
            gi|571330882|gb|AHF27397.1|
            S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine 4'-
            O-methyltransferase 2 [synthetic construct]
          Length = 357

 Score =  408 bits (1048), Expect = e-111
 Identities = 185/338 (54%), Positives = 261/338 (77%), Gaps = 2/338 (0%)
 Frame = -3

Query: 1259 QAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSVNVDHLYRIMR 1080
            QA++W +IYGF++SLVLRCAVE+GIADII  +   I++ QLA+K+P+ +V+ D+LYR++R
Sbjct: 20   QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVR 79

Query: 1079 FLVHMKLFSIQSVKG--ELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAPWHYIKES 906
            +LVH+ +   ++  G  E  Y L+P    L++  ++ MVP IL +T KDFM  WH++KE 
Sbjct: 80   YLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEG 139

Query: 905  LGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEMFEGILSL 726
            LG  S TAFEKG+GM+IW Y+  NP ++QLFNEGMA ++RLLT  LI+DC++ F+G+ SL
Sbjct: 140  LGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGLDSL 199

Query: 725  VDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSIPSADAIMM 546
            VD+GGG GT ++AI +AFPH+KCT+YDLPHV+A+S D   + +VPGDMFKS+PSA AI++
Sbjct: 200  VDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAILL 259

Query: 545  KCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCMDLDMMLNT 366
            K ILHDW D+EC+ ILKKC+EA+P   GKVIIVD+ L+ ES+   +  R  +D+DM++NT
Sbjct: 260  KLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVNT 319

Query: 365  GGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYP 252
            GG+ERT ++W+ L+  AGF  +KI+ I A+QSVIEA+P
Sbjct: 320  GGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357


>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
            4'-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:3'-hydroxy-N-
            methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
            [Coptis japonica] gi|9711916|dbj|BAB08005.1|
            S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
            4'-O-methyltransferase [Coptis japonica]
          Length = 350

 Score =  406 bits (1044), Expect = e-110
 Identities = 194/341 (56%), Positives = 265/341 (77%), Gaps = 1/341 (0%)
 Frame = -3

Query: 1271 DQAAQAKIWRLIYGFSESLVLRCAVELGIADIIHEHGDTISIHQLASKIPVNSVNVDHLY 1092
            D  AQA +W++IYGF++SLVLRCAVELGI DII  +   +++  LASK+PV+ VN D+LY
Sbjct: 11   DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNLY 70

Query: 1091 RIMRFLVHMKLFSIQ-SVKGELRYGLEPAARCLVKGWDKCMVPSILSITDKDFMAPWHYI 915
            RI+R+LV M++  ++ S  G+ +Y LEP A  L +   + MVP IL +T KDFM PWH +
Sbjct: 71   RILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHSM 130

Query: 914  KESLGKESPTAFEKGLGMNIWDYMADNPKKNQLFNEGMACDSRLLTSALIQDCKEMFEGI 735
            K+ L  ++ TAFEK +GM IW+Y+  +P ++QLFNEGMA ++RLLTS+LI   ++MF+GI
Sbjct: 131  KDGLS-DNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQGI 189

Query: 734  LSLVDVGGGTGTALRAIAKAFPHMKCTVYDLPHVIADSPDYAEVARVPGDMFKSIPSADA 555
             SLVDVGGG GT ++AI+ AFPH+KCT++DLPHVIA+S D   + R+ GDMFKS+PSA A
Sbjct: 190  DSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPSAQA 249

Query: 554  IMMKCILHDWDDQECIKILKKCREAVPPNGGKVIIVDIVLDVESDSHYSNMRRCMDLDMM 375
            I++K ILHDW+D++ IKILK+CR AVP +GGKVIIVD+ LD ESD   S+ R  +D+DM+
Sbjct: 250  IILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDIDML 309

Query: 374  LNTGGKERTEEEWKKLIYDAGFTGYKIKSISAVQSVIEAYP 252
            +NTGGKERT+E W+K++  AGF+G KI+ I+A+QSVIE +P
Sbjct: 310  VNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350