BLASTX nr result
ID: Cinnamomum23_contig00001532
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cinnamomum23_contig00001532
(1331 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 488 e-135
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 486 e-134
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 460 e-126
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 458 e-126
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 456 e-125
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 454 e-125
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 454 e-125
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 453 e-124
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 453 e-124
gb|AFK73713.1| cytochrome P450 [Papaver somniferum] 373 e-100
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum] 370 1e-99
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 367 9e-99
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 365 4e-98
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 355 6e-95
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 355 6e-95
ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 342 4e-91
ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 335 5e-89
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 334 8e-89
ref|XP_002283461.2| PREDICTED: probable (S)-N-methylcoclaurine 3... 333 1e-88
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 333 1e-88
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 [Coptis japonica]
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 488 bits (1257), Expect = e-135
Identities = 238/384 (61%), Positives = 288/384 (75%), Gaps = 24/384 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV H++NS+ WS+CND WK+LR++CRTELF+PKM E+Q+ +RE K +MV FL+GKE
Sbjct: 101 SFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKE 160
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G+VVKI EVVFGTL NIFGNLI ADYF
Sbjct: 161 GEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYF 220
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKKGNDTNNDFVRVLLDAELNDHQIN 864
P++GK+DL GQRR VA+ LQQIYD+W V+++ERR KG+++ NDFV VLL+A L+D +IN
Sbjct: 221 PIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKIN 280
Query: 863 ALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQ 684
ALL+E+F AGTET+ASTIEWAI ELTK P ++SK+R E+ +VGDN +KES LPHLPYLQ
Sbjct: 281 ALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQ 340
Query: 683 ACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSP 504
A VKE +ALE C ++ YTIPK+CQ+MVNAWAIGRDPKTW DPLNF P
Sbjct: 341 AFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKP 400
Query: 503 ERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSE 324
ERFL+S+VD+KGNDFELIPFG GRRICPG+PLA+QF LIV T+VQ+FEW L GM SE
Sbjct: 401 ERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSE 460
Query: 323 LVMDEKFGLTLQKEPPLLIVPKAR 252
L MDEKFGLTLQK+PPLLIV KAR
Sbjct: 461 LSMDEKFGLTLQKDPPLLIVLKAR 484
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 486 bits (1251), Expect = e-134
Identities = 237/384 (61%), Positives = 285/384 (74%), Gaps = 24/384 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV H++NS+ WS+CND WK+LR++CRTELF+PKM E+Q+ +RE K +MV FL+ KE
Sbjct: 101 SFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKE 160
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G+VVKI EVVFGTL NIFGNLI ADYF
Sbjct: 161 GEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYF 220
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKKGNDTNNDFVRVLLDAELNDHQIN 864
P++GK DL GQRR VAD LQQIYD+W + ++ERR KG+++NNDFV VLL+A L D +IN
Sbjct: 221 PIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESNNDFVDVLLNAGLEDQKIN 280
Query: 863 ALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQ 684
ALL+E+F GTET+ASTIEWAI ELTK P ++SK+R E+ +VGDN +KES LPHLPYLQ
Sbjct: 281 ALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQ 340
Query: 683 ACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSP 504
A VKE +ALE C ++ YTIPK+CQ+MVNAWAIGRDPKTW DPLNF P
Sbjct: 341 AFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKP 400
Query: 503 ERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSE 324
ERFL+S+VD+KGNDFELIPFG GRRICPG+PLA+QF+ LIV T+VQ+FEW L GM SE
Sbjct: 401 ERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFISLIVATLVQNFEWSLPQGMSTSE 460
Query: 323 LVMDEKFGLTLQKEPPLLIVPKAR 252
L MDEKFGLTLQK+PPLLIV KAR
Sbjct: 461 LSMDEKFGLTLQKDPPLLIVLKAR 484
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 460 bits (1184), Expect = e-126
Identities = 223/386 (57%), Positives = 277/386 (71%), Gaps = 26/386 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV H++NS+ WS+CN+ WK LR++CR ELF+ KM E+QA +RE K +MV FLK +
Sbjct: 100 SFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQ 159
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G VKI EVVFGTL NIFGNLI ADYF
Sbjct: 160 GSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYF 219
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKK--GNDTNNDFVRVLLDAELNDHQ 870
P LG+ DL GQR+ VADCLQ IY +W +++ER+ K N NDFV +LLD+ L+D Q
Sbjct: 220 PFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQ 279
Query: 869 INALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPY 690
INALL+E+F AGTET+ASTIEWA++ELTK+P + + +R E+ ++VG P+KES +P++PY
Sbjct: 280 INALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPY 339
Query: 689 LQACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNF 510
LQA VKE +ALE C++L YTIPK+CQ+MVNAW IGRDPK W DPL F
Sbjct: 340 LQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKF 399
Query: 509 SPERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQP 330
+PERFLNS++DFKGNDFELIPFG+GRRICPGVPLATQF+ LIV T+VQ+F+WGL GM P
Sbjct: 400 APERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDP 459
Query: 329 SELVMDEKFGLTLQKEPPLLIVPKAR 252
S+L+M+EKFGLTLQKEPPL IVPK R
Sbjct: 460 SQLIMEEKFGLTLQKEPPLYIVPKNR 485
>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 458 bits (1179), Expect = e-126
Identities = 223/386 (57%), Positives = 276/386 (71%), Gaps = 26/386 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV H++NS+ WS+CN+ WK LR++CRTELF+ KM E+QA +RE K +MV +LK
Sbjct: 95 SFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNV 154
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G VKIAEVVFGTL NIFGNLI ADYF
Sbjct: 155 GNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYF 214
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKK--GNDTNNDFVRVLLDAELNDHQ 870
P LGK DL GQ + VADCLQ IY +W +++ER+ K N NDFV +LLD+ L+D Q
Sbjct: 215 PFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQ 274
Query: 869 INALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPY 690
INALL+E+F AGTET+ASTIEWA++ELTK+P + + +R E+ ++VG P+KES +P++PY
Sbjct: 275 INALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPY 334
Query: 689 LQACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNF 510
LQA VKE +ALE C++L YTIPK+CQ+MVNAW IGRDPK W DPL F
Sbjct: 335 LQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKF 394
Query: 509 SPERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQP 330
SPERFLNS++DFKGNDFELIPFG+GRRICPGVPLATQF+ LIV ++VQ+F+WG GM P
Sbjct: 395 SPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDP 454
Query: 329 SELVMDEKFGLTLQKEPPLLIVPKAR 252
S+L+M+EKFGLTLQKEPPL IVPK R
Sbjct: 455 SQLIMEEKFGLTLQKEPPLYIVPKTR 480
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 456 bits (1174), Expect = e-125
Identities = 222/386 (57%), Positives = 276/386 (71%), Gaps = 26/386 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV H++NS+ WS+CN+ WK LR++CRT+LF+ KM E+QA +RE K +MV +LK
Sbjct: 95 SFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNV 154
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G VKIAEVVFGTL NIFGNLI ADYF
Sbjct: 155 GNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYF 214
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKK--GNDTNNDFVRVLLDAELNDHQ 870
P LGK DL GQ + VADCLQ IY +W +++E + K N NDFV +LLD+ L+D Q
Sbjct: 215 PFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQHNNSKKNDFVEILLDSGLDDQQ 274
Query: 869 INALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPY 690
INALL+E+F AGTET+ASTIEWA++ELTK+P + + +R E+ ++VG P+KES +P++PY
Sbjct: 275 INALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPY 334
Query: 689 LQACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNF 510
LQA VKE +ALE C++L YTIPK+CQ+MVNAW IGRDPK W DPL F
Sbjct: 335 LQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKF 394
Query: 509 SPERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQP 330
SPERFLNS++DFKGNDFELIPFG+GRRICPGVPLATQF+ LIV ++VQ+F+WGL GM P
Sbjct: 395 SPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGLPKGMDP 454
Query: 329 SELVMDEKFGLTLQKEPPLLIVPKAR 252
S+L+M+EKFGLTLQKEPPL IVPK R
Sbjct: 455 SQLIMEEKFGLTLQKEPPLYIVPKTR 480
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 454 bits (1167), Expect = e-125
Identities = 226/384 (58%), Positives = 274/384 (71%), Gaps = 24/384 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV H+ NS+ WS+CN+ WK+LR++CRTELFSPKM E+QA++RE K MV FL+ KE
Sbjct: 102 SFRVYEHVLNSIVWSECNENWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKE 161
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
Q VKI EVVF TL NIFGNLI ADYF
Sbjct: 162 NQEVKIVEVVFNTLVNIFGNLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYF 221
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKKGNDTNNDFVRVLLDAELNDHQIN 864
P++GK DL GQRR VA L+QIYD+W V+++ERR G+ NDF VLL+A L+D +IN
Sbjct: 222 PIMGKFDLFGQRREVAKVLKQIYDVWGVMLKERRSITGH-RENDFANVLLNAGLDDQKIN 280
Query: 863 ALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQ 684
ALL+E+F AGTETTASTIEWAI ELT + ++SKLR E+ +VG +KES++PHLPYLQ
Sbjct: 281 ALLMELFGAGTETTASTIEWAITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQ 340
Query: 683 ACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSP 504
A VKE +ALE C+++ YTIPK+CQ+MVNAWAIGRDPKTW DPL F P
Sbjct: 341 AIVKETLRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVNAWAIGRDPKTWDDPLTFKP 400
Query: 503 ERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSE 324
ERF+NS VD+KGNDFELIPFG GRRICPG+PLA+QFL LIV T+VQ+F+W GM P+E
Sbjct: 401 ERFMNSTVDYKGNDFELIPFGGGRRICPGLPLASQFLSLIVATLVQNFDWSFPQGMTPNE 460
Query: 323 LVMDEKFGLTLQKEPPLLIVPKAR 252
+ MDEKFGL LQK+PPLLIV KAR
Sbjct: 461 VPMDEKFGLPLQKDPPLLIVLKAR 484
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1, partial [Eschscholzia
californica] gi|3127027|gb|AAC39452.1|
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 487
Score = 454 bits (1167), Expect = e-125
Identities = 223/388 (57%), Positives = 277/388 (71%), Gaps = 27/388 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV H++NS+ WSDC + WK LR++CRTELF+ KM E+QAH+RE+K +MV +L K+
Sbjct: 100 SFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQ 159
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G+ VKI EV+FGTL NIFGNLI ADYF
Sbjct: 160 GEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYF 219
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKK---GNDTNNDFVRVLLDAELNDH 873
P+LGK DL GQR+ VA+CL+ IY IW +++ER+ K G + NDFV V LD+ LND+
Sbjct: 220 PMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDY 279
Query: 872 QINALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLP 693
QINALL+E+F AGTET+ASTIEWA+ ELTK+P I +K+R EIQT+VG+ +KES P+LP
Sbjct: 280 QINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLP 339
Query: 692 YLQACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLN 513
YL+A VKE +ALE C +L YTIPKDCQ+MVNAW IGRDPKTW DPL
Sbjct: 340 YLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLT 399
Query: 512 FSPERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQ 333
FSPERFLNS+VDF+GNDF LIPFG+GRRICPG+P+A QF+ L+V T VQ+ +W L NGM
Sbjct: 400 FSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMS 459
Query: 332 PSELVMDEKFGLTLQKEPPLLIVPKARV 249
L+++EKFGLTLQKEPPL IVPK+RV
Sbjct: 460 VDHLIVEEKFGLTLQKEPPLFIVPKSRV 487
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 453 bits (1166), Expect = e-124
Identities = 223/388 (57%), Positives = 277/388 (71%), Gaps = 27/388 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV H++NS+ WSDC + WK LR++CRTELF+ KM E+QAH+RE+K +MV +L K+
Sbjct: 101 SFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQ 160
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G+ VKI EV+FGTL NIFGNLI ADYF
Sbjct: 161 GEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYF 220
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKK---GNDTNNDFVRVLLDAELNDH 873
P+LGK DL GQR+ VA+CL+ IY IW +++ER+ K G + NDFV V LD+ LND+
Sbjct: 221 PMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDY 280
Query: 872 QINALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLP 693
QINALL+E+F AGTET+ASTIEWA+ ELTK+P I +KLR E+QT+VG+ +KES P+LP
Sbjct: 281 QINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLP 340
Query: 692 YLQACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLN 513
YL+A VKE +ALE C +L YTIPKDCQ+MVNAW IGRDPKTW DPL
Sbjct: 341 YLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLT 400
Query: 512 FSPERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQ 333
FSPERFLNS+VDF+GNDF LIPFG+GRRICPG+P+A QF+ L+V T VQ+ +W L NGM
Sbjct: 401 FSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMS 460
Query: 332 PSELVMDEKFGLTLQKEPPLLIVPKARV 249
L+++EKFGLTLQKEPPL IVPK+RV
Sbjct: 461 VDHLIVEEKFGLTLQKEPPLFIVPKSRV 488
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2 [Eschscholzia
californica] gi|3127029|gb|AAC39453.1|
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 488
Score = 453 bits (1166), Expect = e-124
Identities = 224/388 (57%), Positives = 278/388 (71%), Gaps = 27/388 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV H++NS+ WSDC + WK LR++CRTELF+ KM E+QAH+RE+K +MV +L K+
Sbjct: 101 SFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQ 160
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G+ VKI EV+FGTL NIFGNLI ADYF
Sbjct: 161 GEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYF 220
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKK---GNDTNNDFVRVLLDAELNDH 873
P+LGK DL GQR+ VA+CL+ IY IW +++ER+ K G + NDFV V LD+ LND+
Sbjct: 221 PMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDY 280
Query: 872 QINALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLP 693
QINALL+E+F AGTET+ASTIEWA+ ELTK+P I +KLR E+QT+VG+ +KES P+LP
Sbjct: 281 QINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLP 340
Query: 692 YLQACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLN 513
YL+A VKE +ALE C +L YTIPKDCQ+MVNAW IGRDPKTW DPL
Sbjct: 341 YLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLT 400
Query: 512 FSPERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQ 333
FSPERFLNS+VDF+GNDF LIPFG+GRRICPG+P+A QF+ L+V T VQ+ +W L NGM
Sbjct: 401 FSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMS 460
Query: 332 PSELVMDEKFGLTLQKEPPLLIVPKARV 249
L+++EKFGLTLQKEPPL IVPK+RV
Sbjct: 461 VDHLIVEEKFGLTLQKEPPLFIVPKSRV 488
>gb|AFK73713.1| cytochrome P450 [Papaver somniferum]
Length = 440
Score = 373 bits (957), Expect = e-100
Identities = 183/334 (54%), Positives = 231/334 (69%), Gaps = 27/334 (8%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFRV +H++NS+ W++CN+ WK LR++CRT+LF+ KM E QA +RE K +MV +LK E
Sbjct: 101 SFRVKNHVENSIVWNECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNE 160
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G VKI EV+FGTL N+FGNLI DY
Sbjct: 161 GIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYV 220
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKK---GNDTNNDFVRVLLDAELNDH 873
P+LG +DL GQR+ VADCLQ +Y +W +++ERR K DT NDFV VLLD+ L+D
Sbjct: 221 PLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQ 280
Query: 872 QINALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLP 693
QIN+LLL+MF+AGTETTASTIEWA+ ELTK+P + + +R E+ ++VG +KES + +LP
Sbjct: 281 QINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLP 340
Query: 692 YLQACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLN 513
YLQA VKE +ALE CQ+L YTIPK+CQ+MVNAW IGRDPKTW DPL
Sbjct: 341 YLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLK 400
Query: 512 FSPERFLNSNVDFKGNDFELIPFGSGRRICPGVP 411
FSP+RFLNS++DFKGNDFELIPFG+GRRICPGVP
Sbjct: 401 FSPDRFLNSSIDFKGNDFELIPFGAGRRICPGVP 434
>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 370 bits (951), Expect = 1e-99
Identities = 183/383 (47%), Positives = 254/383 (66%), Gaps = 22/383 (5%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFR+ H+ S+ W+DC++YWK+LR+I RTE+FSPKM + Q H+RE+KVA++V FLKGKE
Sbjct: 102 SFRLKHHIKYSLVWADCSEYWKLLRKIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKE 161
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXXAD----------------------YFPV 1038
GQVVKI+++VFGTL NI GN+I AD ++P+
Sbjct: 162 GQVVKISQLVFGTLLNILGNVIFSKDVFRYVGADGDKMQTLIKEMLMIGAAPNLAEFWPI 221
Query: 1037 LGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKKGNDTNNDFVRVLLDAELNDHQINAL 858
+ DL G ++ ++ +W + ER+ K+ N+ D + VLL + ND QINA+
Sbjct: 222 FEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVKR-NEEPKDMLDVLLLNDFNDAQINAV 280
Query: 857 LLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQAC 678
LE F G+ET+++T EWA+AEL K+P IL+K+RQE+ +VG + +KES LPHLPYLQAC
Sbjct: 281 FLETFGPGSETSSATSEWALAELIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYLQAC 340
Query: 677 VKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPER 498
+KE H+A+E C ++GYTIPKD QV+VN +AIGRDPKTWK+P F PER
Sbjct: 341 IKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQVLVNIYAIGRDPKTWKEPTTFKPER 400
Query: 497 FLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSELV 318
FL S+VD+ G FE IPFG+GRR C G+PLAT+ + LIV +VQ+ +W L +G P +LV
Sbjct: 401 FLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIPLIVSNLVQTLDWSLPDGKLPQDLV 460
Query: 317 MDEKFGLTLQKEPPLLIVPKARV 249
M+E L+L +P L ++PK+RV
Sbjct: 461 MNESLSLSLALDPTLTVIPKSRV 483
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 367 bits (943), Expect = 9e-99
Identities = 180/385 (46%), Positives = 261/385 (67%), Gaps = 25/385 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
S R+ ++++SM W+DC DYWKM+R++ RTELFS KM + QAH REEKV++++ FL KE
Sbjct: 101 SVRIKGYIEHSMVWADCTDYWKMVRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKE 160
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G+ V A+V+FG++ NI G LI AD +
Sbjct: 161 GEKVNFADVIFGSILNILGALIFSKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLY 220
Query: 1043 PVLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKKGNDTNNDFVRVLLDAELNDHQIN 864
P+LG D G R+ A C++++ + W I+++RR K + + NDF++VLLD+ +D QI+
Sbjct: 221 PILGGSDFQGLRKASAACVKRMNESWAAIVKQRR-KNDDHSKNDFLQVLLDSGFSDPQID 279
Query: 863 ALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVG-DNPIKESHLPHLPYL 687
A+LLE F G++T+ STIEWA+AEL ++P L K+R+E+ ++ N +KES LP+LPYL
Sbjct: 280 AMLLETFGPGSDTSTSTIEWAMAELLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYL 339
Query: 686 QACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFS 507
ACVKE H+A+E CQ++ YTIPK CQ+MVN +AIGRD KTW+ PL+F
Sbjct: 340 HACVKETLRLHPPVTFLLPHRAMETCQMMNYTIPKGCQLMVNTYAIGRDSKTWEKPLSFL 399
Query: 506 PERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPS 327
PERFLNS +D++GNDF+ IPFG+GRRICPG+ LAT+ ++LI+ +++ +F+W L +GM P
Sbjct: 400 PERFLNSELDYQGNDFQYIPFGAGRRICPGLSLATRVVRLILASLLHTFDWSLPDGMHPD 459
Query: 326 ELVMDEKFGLTLQKEPPLLIVPKAR 252
EL M++KFGL LQK+ PL+++PK R
Sbjct: 460 ELDMNDKFGLALQKDIPLVVIPKLR 484
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 365 bits (937), Expect = 4e-98
Identities = 181/383 (47%), Positives = 255/383 (66%), Gaps = 23/383 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
SFR+ H+ S+ WSDC DYWK+LR+I RTE+FSPKM +AQ+H+RE+KVA+++ FL+ KE
Sbjct: 104 SFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKE 163
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX-----------------------ADYFP 1041
GQVVKI++ VFGTL NI GN++ A+++P
Sbjct: 164 GQVVKISQFVFGTLLNILGNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYP 223
Query: 1040 VLGKIDLLGQRRTVADCLQQIYDIWNVIMRERREKKGNDTNNDFVRVLLDAELNDHQINA 861
L ++DL G ++ + ++ +W ++ER+ + N+ + D + VLL + ND QINA
Sbjct: 224 SLEELDLQGLKKKCDERFIRVMKMWEGTVKERKANR-NEESKDMLDVLLANDFNDAQINA 282
Query: 860 LLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQA 681
L LE F G+ET+++TIEW IAEL KSP ++K+R+E+ +VG + IKES LP LPYLQA
Sbjct: 283 LFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQA 342
Query: 680 CVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPE 501
C+KE +A E C+++GYTIPK+ QV+VNA+AIGRDPK+WKDP F PE
Sbjct: 343 CIKEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPE 402
Query: 500 RFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSEL 321
RFL S+VDF G ++ IPFGSGRR C G+PLAT+ + LIVG++V ++++GL G +P +L
Sbjct: 403 RFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDL 462
Query: 320 VMDEKFGLTLQKEPPLLIVPKAR 252
M+E LTL +P L +VPKAR
Sbjct: 463 KMNEMLSLTLAIDPSLCVVPKAR 485
>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223549945|gb|EEF51432.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 355 bits (910), Expect = 6e-95
Identities = 170/376 (45%), Positives = 247/376 (65%), Gaps = 25/376 (6%)
Frame = -1
Query: 1301 SMTWSD-CNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKEGQVVKIAEV 1125
++ W+ CND+WK LR +CRTELFSPK E+QA LRE+K+A+M+ FL K+GQ V +AEV
Sbjct: 120 AIVWNPACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEV 179
Query: 1124 VFGTLFNIFGNLIXXXXXXXXXX---------------------ADYFPVLGKIDLLGQR 1008
VFGT+FN +L+ A+++P+L +D G +
Sbjct: 180 VFGTIFNTISHLLFSTDLIGFENQIGGVKSLLWSMMEMATSPNIAEFYPILAPLDPQGLK 239
Query: 1007 RTVADCLQQIYDIWNVIMRERREKKGND---TNNDFVRVLLDAELNDHQINALLLEMFAA 837
R + CL++++ +W + ++ERR +D DF+ + L +D QIN L++E+ +A
Sbjct: 240 RKMTKCLKEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSA 299
Query: 836 GTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQACVKEXXXX 657
GT+TT++++EWA+AEL K+ + K+R+E+ + NPIKESH+ LPYL ACVKE
Sbjct: 300 GTDTTSTSVEWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLRL 359
Query: 656 XXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPERFLNSNVD 477
+A E C+++ YTIPKD QV+VN WAIGRDP W+DP +F PERFL S++D
Sbjct: 360 HPPAPFLIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLGSSLD 419
Query: 476 FKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSELVMDEKFGL 297
KG+DFELIPFGSGRRICPG+P+AT+ L L++ +++ F+W L NG P+++ M EKFG+
Sbjct: 420 VKGHDFELIPFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEKFGI 479
Query: 296 TLQKEPPLLIVPKARV 249
TLQ E PLLI+PK ++
Sbjct: 480 TLQMEHPLLIIPKPKL 495
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 355 bits (910), Expect = 6e-95
Identities = 172/376 (45%), Positives = 248/376 (65%), Gaps = 25/376 (6%)
Frame = -1
Query: 1301 SMTWS-DCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKEGQVVKIAEV 1125
++ W+ C+D WK LR +CR+ELFS K E+QA LRE+K+ +M+ FL K+GQ+V I EV
Sbjct: 121 ALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEV 180
Query: 1124 VFGTLFNIFGNLIXXXXXXXXXX-----------------------ADYFPVLGKIDLLG 1014
VF T FN NL+ AD++PVL K+D G
Sbjct: 181 VFTTAFNTISNLLFSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQG 240
Query: 1013 QRRTVADCLQQIYDIWNVIMRERREKKGNDTNN-DFVRVLLDAELNDHQINALLLEMFAA 837
+R + +CL++++ +W++ ++ERRE+ D DF+ V L +DHQIN LLLE+F+A
Sbjct: 241 IKRKMKNCLERMFGVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDHQINWLLLELFSA 300
Query: 836 GTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQACVKEXXXX 657
G +TT +T+EWA+AEL K T+L K+R+E++T +G + I+ESH+P L YL ACVKE
Sbjct: 301 GADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACVKETLRL 360
Query: 656 XXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPERFLNSNVD 477
+A E C+++ YTIPK Q++VN WAIGRDP W+DPL++ PERFL+SN+D
Sbjct: 361 HPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERFLDSNLD 420
Query: 476 FKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSELVMDEKFGL 297
FKG++FE +PFGSGRRICPG+P+ T+ L LI+ ++V F+W L NG P+ L M++KF +
Sbjct: 421 FKGHNFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQNGDDPAMLDMNDKFSI 480
Query: 296 TLQKEPPLLIVPKARV 249
TL+KE LL+VPK ++
Sbjct: 481 TLEKEQHLLVVPKRKL 496
>ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Populus euphratica]
Length = 492
Score = 342 bits (877), Expect = 4e-91
Identities = 172/376 (45%), Positives = 240/376 (63%), Gaps = 25/376 (6%)
Frame = -1
Query: 1301 SMTWS-DCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKEGQVVKIAEV 1125
S+ W+ C+D WK LR +CR ELFS K E+QA LRE+K+ +MV F+ ++G+VV I EV
Sbjct: 117 SIVWATQCSDGWKSLRALCRNELFSAKAIESQAVLREKKMGEMVEFIGRRKGEVVGIGEV 176
Query: 1124 VFGTLFNIFGNLIXXXXXXXXXX-----------------------ADYFPVLGKIDLLG 1014
V +FN NL+ +D++P+LG ID G
Sbjct: 177 VLAIVFNTIANLLFSVDLIGLEDDGAATGLKSLMWRMMKLGATPNISDFYPILGGIDPQG 236
Query: 1013 QRRTVADCLQQIYDIWNVIMRERREKKGND-TNNDFVRVLLDAELNDHQINALLLEMFAA 837
+R +A C+ Q++ IW ++ERREK +D +DF+ V L D QIN L LE+ +A
Sbjct: 237 LKRKMAVCVNQMFGIWGKYIKERREKHVHDGPRSDFLDVFLANGFEDLQINWLALELLSA 296
Query: 836 GTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQACVKEXXXX 657
GT+TTA+T+EWAIAEL K+ +L K+ +EI+ N +KESH+ LPYL ACV E
Sbjct: 297 GTDTTATTVEWAIAELLKNKEVLKKVSEEIKRETDTNSLKESHVSQLPYLNACVNETLRL 356
Query: 656 XXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPERFLNSNVD 477
+ALE C+++ YTIP+D +V+VN WA+GRDP W+DPL+F PERFL S++D
Sbjct: 357 HPPVPFLIPRRALETCKVMDYTIPRDSEVIVNVWAVGRDPSLWEDPLSFKPERFLGSDLD 416
Query: 476 FKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSELVMDEKFGL 297
FKG+DFE +PFG+GRRICPG+P+A + + LI+ T++ F+W L NG P+ L M EKFG+
Sbjct: 417 FKGHDFEFLPFGAGRRICPGLPMAAKQVHLIIATLLYYFDWSLPNGEDPATLDMSEKFGI 476
Query: 296 TLQKEPPLLIVPKARV 249
TLQKE PLL+VP+ R+
Sbjct: 477 TLQKEQPLLLVPRRRI 492
>ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 335 bits (859), Expect = 5e-89
Identities = 170/386 (44%), Positives = 245/386 (63%), Gaps = 26/386 (6%)
Frame = -1
Query: 1331 SFRVPSHLDNSMTWSDCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKE 1152
S RV +++ SM W+DC DYWKM+R+I RTELFS KM + AH+REEKV++++ FL+ KE
Sbjct: 101 SVRVKGYIEYSMVWADCTDYWKMVRKILRTELFSTKMLDVHAHVREEKVSELMKFLRRKE 160
Query: 1151 GQVVKIAEVVFGTLFNIFGNLIXXXXXXXXXX------------------------ADYF 1044
G+ V +V+FG + N+ G LI AD +
Sbjct: 161 GEEVNFVDVIFGCILNMLGALIYSKDVYDFEDKTDINLGMKGMIRQLMILAATPNIADLY 220
Query: 1043 PVL-GKIDLLGQRRTVADCLQQIYDIWNVIMRERREKKGNDTNNDFVRVLLDAELNDHQI 867
P+ D G R+ A C++++ + W I+ ERR K + T ND ++VLLD+ +D QI
Sbjct: 221 PIFFDGSDFQGLRKESAACVKRMSESWAAIINERR-KNNDHTKNDLLQVLLDSGFSDPQI 279
Query: 866 NALLLEMFAAGTETTASTIEWAIAELTKSPTILSKLRQEIQTIVG-DNPIKESHLPHLPY 690
+A+ LE F G++T+ASTIEWA+AEL ++P L KL +E+ ++G +N +K+S LP+LPY
Sbjct: 280 DAIFLETFGPGSDTSASTIEWALAELLRNPEKLVKLHEELDRVIGRNNTVKDSDLPNLPY 339
Query: 689 LQACVKEXXXXXXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNF 510
L ACVKE H ALE C+++ YTIPK +V+VN +AIGRDP TW +P +F
Sbjct: 340 LHACVKETLRLHPPVPFLIPHIALESCEVMNYTIPKGSEVLVNLYAIGRDPTTWDNPNSF 399
Query: 509 SPERFLNSNVDFKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQP 330
PERFLNS VD++GN F+ IPFG+GRR+CPG+ L T+ ++LI+ +V +F+W L GM
Sbjct: 400 LPERFLNSEVDYQGNHFQYIPFGAGRRMCPGMSLGTRVVRLILAALVHTFDWSLPGGMHQ 459
Query: 329 SELVMDEKFGLTLQKEPPLLIVPKAR 252
EL M ++FG+ QKE PL+++P R
Sbjct: 460 DELDMADRFGVGFQKETPLVVIPTLR 485
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 334 bits (857), Expect = 8e-89
Identities = 172/379 (45%), Positives = 233/379 (61%), Gaps = 25/379 (6%)
Frame = -1
Query: 1286 DCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKEGQVVKIAEVVFGTLF 1107
DCN WK LR ICR ELFS K E+Q LRE KV ++V FL KEG+VVK+ ++VF T+
Sbjct: 668 DCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTIC 727
Query: 1106 NIFGNLIXXXXXXXXXX------------------------ADYFPVLGKIDLLGQRRTV 999
NI N +DY+P+LG +D+ G R
Sbjct: 728 NILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKA 787
Query: 998 ADCLQQIYDIWNVIMRERREKKGN-DTNNDFVRVLLDAELNDHQINALLLEMFAAGTETT 822
+ ++I W I++ERR ++ N ++ DF+ LL+ D QIN ++LE+F+AG ET+
Sbjct: 788 KEMFERIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETS 847
Query: 821 ASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQACVKEXXXXXXXXX 642
+ T+EWA+AEL ++ + KLR E++ IVG++P++ESHLP LPYLQACVKE
Sbjct: 848 SLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPPAP 907
Query: 641 XXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPERFLNSNVDFKGND 462
H A E CQ++GYTIPKD Q+ VN WA+ RDPK W DPL+F PERFL+S +DFKGND
Sbjct: 908 LLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGND 967
Query: 461 FELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSELVMDEKFGLTLQKE 282
FE IPFG+GRRICPG+ L + + LI+ T V F W L M ++L M+E +TL+KE
Sbjct: 968 FEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKE 1027
Query: 281 PPLLIVPKARV*LLTYFVF 225
PL +VP+ R YF+F
Sbjct: 1028 QPLRLVPRVR----KYFIF 1042
Score = 332 bits (850), Expect = 5e-88
Identities = 161/365 (44%), Positives = 233/365 (63%), Gaps = 20/365 (5%)
Frame = -1
Query: 1286 DCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKEGQVVKIAEVVFGTLF 1107
+C + WK LR ICRTE+FS K E+ LRE KV ++V FL KEG+VVK+ ++VF T+
Sbjct: 1173 ECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTIC 1232
Query: 1106 NIFGNLIXXXXXXXXXX-------------------ADYFPVLGKIDLLGQRRTVADCLQ 984
NI N +D++P+L ++L G ++ + L
Sbjct: 1233 NILSNTFFSMDLCDFEREGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALG 1292
Query: 983 QIYDIWNVIMRERREKKG-NDTNNDFVRVLLDAELNDHQINALLLEMFAAGTETTASTIE 807
+I W ++ERR++K ++ D + L+ D QIN ++LE+F+AG +T+ TIE
Sbjct: 1293 RILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIE 1352
Query: 806 WAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQACVKEXXXXXXXXXXXXXH 627
WAI +L ++P ++ KLR E+ I+G++P++ESHLPHLPYLQACVKE H
Sbjct: 1353 WAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVKETLRLHPPAPLLLPH 1412
Query: 626 KALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPERFLNSNVDFKGNDFELIP 447
+A+E CQ++GYTIPKD QV VN WA+GRDPK W DPL+F+PERFL+S ++FKGNDFE IP
Sbjct: 1413 RAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEYIP 1472
Query: 446 FGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSELVMDEKFGLTLQKEPPLLI 267
FG+GRRICPG+ L + + L++ T+V F+W L + M +++ M+E +TL+KE PL +
Sbjct: 1473 FGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKENPLRL 1532
Query: 266 VPKAR 252
VPK R
Sbjct: 1533 VPKVR 1537
Score = 322 bits (826), Expect = 3e-85
Identities = 159/366 (43%), Positives = 234/366 (63%), Gaps = 24/366 (6%)
Frame = -1
Query: 1283 CNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKEGQVVKIAEVVFGTLFN 1104
CN W+ L+ ICR ELFS K E++ LRE KV ++V FL+ KEG+VVK+ ++V+ T+ N
Sbjct: 129 CNSDWRNLKAICRMELFSGKAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCN 188
Query: 1103 IFGN-----------------------LIXXXXXXXXXXADYFPVLGKIDLLGQRRTVAD 993
I N L D++P+LG +D+ G R+ + +
Sbjct: 189 ILSNKFFSIDFSDFEGRDVRGVLLKDLLNENAELGATNILDFYPILGGLDIQGIRKKLKE 248
Query: 992 CLQQIYDIWNVIMRERREKK-GNDTNNDFVRVLLDAELNDHQINALLLEMFAAGTETTAS 816
++I W I++ERR+++ ++ DF+ LL+ D QIN +++E+F AG ET++
Sbjct: 249 IFRRIPTTWEDILKERRKQRIHGSSHGDFLDALLETGFEDDQINHVIMELFFAGPETSSL 308
Query: 815 TIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQACVKEXXXXXXXXXXX 636
T+EWA+AEL K+ + KL E+ I+G++P++ESHLPHLPYLQACVKE
Sbjct: 309 TVEWAMAELIKNQDAMHKLCNELTQIIGESPVRESHLPHLPYLQACVKETLRLHPTGPLL 368
Query: 635 XXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPERFLNSNVDFKGNDFE 456
H+A E CQ++GYTIPKD + VN WA+GRDP TW+DPL+F PERFL+S ++FKGNDFE
Sbjct: 369 LPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLSFKPERFLDSKLEFKGNDFE 428
Query: 455 LIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSELVMDEKFGLTLQKEPP 276
IPFG+GRR+CPG+PLA + + +I+ T V+ F+W M +E+ M+E+F +TL+KE P
Sbjct: 429 YIPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTPGDMDFAEIDMEERFVITLRKEQP 488
Query: 275 LLIVPK 258
L ++ K
Sbjct: 489 LRLLKK 494
>ref|XP_002283461.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 540
Score = 333 bits (855), Expect = 1e-88
Identities = 167/375 (44%), Positives = 232/375 (61%), Gaps = 25/375 (6%)
Frame = -1
Query: 1301 SMTWS-DCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKEGQVVKIAEV 1125
S+ W+ +CND WK LR +CR ELFS K+ E+QA + E+KV +MV F+ KEG+V+K+ EV
Sbjct: 165 SLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEV 224
Query: 1124 VFGTLFNIFGNLIXXXXXXXXXXA-----------------------DYFPVLGKIDLLG 1014
VF T+ N ++ D++ + +DL G
Sbjct: 225 VFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQG 284
Query: 1013 QRRTVADCLQQIYDIWNVIMRERREKKGND-TNNDFVRVLLDAELNDHQINALLLEMFAA 837
+ + + +I +W ++RERRE +D + DF+ +L+ + +D QIN L +E+ A
Sbjct: 285 LNKKTKELIARICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTA 344
Query: 836 GTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQACVKEXXXX 657
G +T++ST+EWA+AEL KSP + K+ +E+ + DN K S LPHLPYLQACVKE
Sbjct: 345 GADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVKETLRL 404
Query: 656 XXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPERFLNSNVD 477
+A C+++ YTIPKD Q+ VNAWAIGRDP W+DPL F PERFLNS VD
Sbjct: 405 HPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVD 464
Query: 476 FKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSELVMDEKFGL 297
FKGN+ E IPFG+GRRICPG+P+A + L LI+ ++ F+W L NG P EL M++KFG+
Sbjct: 465 FKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGI 524
Query: 296 TLQKEPPLLIVPKAR 252
LQKE PLLI+PK R
Sbjct: 525 ALQKEQPLLIIPKVR 539
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 333 bits (855), Expect = 1e-88
Identities = 167/375 (44%), Positives = 232/375 (61%), Gaps = 25/375 (6%)
Frame = -1
Query: 1301 SMTWS-DCNDYWKMLRRICRTELFSPKMTEAQAHLREEKVAQMVGFLKGKEGQVVKIAEV 1125
S+ W+ +CND WK LR +CR ELFS K+ E+QA + E+KV +MV F+ KEG+V+K+ EV
Sbjct: 561 SLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEV 620
Query: 1124 VFGTLFNIFGNLIXXXXXXXXXXA-----------------------DYFPVLGKIDLLG 1014
VF T+ N ++ D++ + +DL G
Sbjct: 621 VFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQG 680
Query: 1013 QRRTVADCLQQIYDIWNVIMRERREKKGND-TNNDFVRVLLDAELNDHQINALLLEMFAA 837
+ + + +I +W ++RERRE +D + DF+ +L+ + +D QIN L +E+ A
Sbjct: 681 LNKKTKELIARICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTA 740
Query: 836 GTETTASTIEWAIAELTKSPTILSKLRQEIQTIVGDNPIKESHLPHLPYLQACVKEXXXX 657
G +T++ST+EWA+AEL KSP + K+ +E+ + DN K S LPHLPYLQACVKE
Sbjct: 741 GADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVKETLRL 800
Query: 656 XXXXXXXXXHKALEPCQLLGYTIPKDCQVMVNAWAIGRDPKTWKDPLNFSPERFLNSNVD 477
+A C+++ YTIPKD Q+ VNAWAIGRDP W+DPL F PERFLNS VD
Sbjct: 801 HPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVD 860
Query: 476 FKGNDFELIPFGSGRRICPGVPLATQFLQLIVGTMVQSFEWGLFNGMQPSELVMDEKFGL 297
FKGN+ E IPFG+GRRICPG+P+A + L LI+ ++ F+W L NG P EL M++KFG+
Sbjct: 861 FKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGI 920
Query: 296 TLQKEPPLLIVPKAR 252
LQKE PLLI+PK R
Sbjct: 921 ALQKEQPLLIIPKVR 935