BLASTX nr result
ID: Cimicifuga21_contig00035558
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cimicifuga21_contig00035558
(1372 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel prot... 296 1e-77
ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel prot... 296 1e-77
emb|CAN67321.1| hypothetical protein VITISV_039348 [Vitis vinifera] 272 1e-70
ref|XP_002889201.1| mechanosensitive ion channel domain-containi... 256 1e-65
ref|NP_177982.1| mechanosensitive channel of small conductance-l... 252 1e-64
>ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
sativus]
Length = 923
Score = 296 bits (757), Expect = 1e-77
Identities = 196/455 (43%), Positives = 252/455 (55%), Gaps = 28/455 (6%)
Frame = +3
Query: 90 MDSLRKSFKQIGSQVHT-KISAGDRVGAVGVEESKPILSDLDD--MKSIDSSGRKDYTSR 260
MD KSFK S + K+S D A E PILSD M DSS RK+ +
Sbjct: 1 MDFSLKSFKSHPSYKYVRKLSGAD---ATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVK 57
Query: 261 DLELXXXXXXE---VNHETKIWRGSSYEFWSD---------GGSKVGTSNGFDFRXXXXX 404
E VN IWR SSY+FW+D GG+K T++ F+FR
Sbjct: 58 VDEADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAK--TTDDFEFRQHRKD 115
Query: 405 XXXXXXADPPSKLIGQFLHKQKASGDFSLDMDLEMEELSVDTHDGGAGLRTITETPS--T 578
DPPSKLIGQFLHKQKASG+ SLDMD+EM EL D L T+ E+P +
Sbjct: 116 VE-----DPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDK----TPLSTVAESPMRRS 166
Query: 579 PKEIRVSFQD----REEEALPSREDDGIXXXXXXXXXXXXXXXXXXXEGRGDRG--EILR 740
+E++VSF+ E +++ R D D G E+LR
Sbjct: 167 SRELKVSFESISEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLR 226
Query: 741 CTXXXXXXXXXXX-----FLRTKTTKSRLMDPPTPRDDAQDKTSGKPLKSRQLKSGFMGS 905
C+ LR KT KSRL+DPP + QD+ SG+ KS Q++SG +
Sbjct: 227 CSSNSSFQRDVSFQRKSSLLRAKT-KSRLLDPP----EHQDRRSGRVPKSGQVRSGLISK 281
Query: 906 TIDEVEEDPFADEDLPEEFKKGKFNTLTVCQWVSLILILAAFACSVSIPFXXXXXXXXXX 1085
+DE ++DPF +EDLP+E+KK LT+ QW SLILI+AA C+++I +
Sbjct: 282 ALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLE 341
Query: 1086 XXXXXXXXXXXFCGRLLSGWGIRLVVFFIERNFLMRKRVLYFVYGVRKAVQNCLWLGLVL 1265
CGRL+SGWGIR++VFFIERNFL+RKRVLYFVYGVRKAVQNCLWLGLVL
Sbjct: 342 VWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVL 401
Query: 1266 LAWHYILDAKVKNEIKSSPLPFFVTKVLVCLLVAT 1370
+AW+++ D KV+ E+KS+ L +VTKVLVCLLV+T
Sbjct: 402 IAWNFLFDDKVQREVKSNALE-YVTKVLVCLLVST 435
>ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
sativus]
Length = 923
Score = 296 bits (757), Expect = 1e-77
Identities = 196/455 (43%), Positives = 252/455 (55%), Gaps = 28/455 (6%)
Frame = +3
Query: 90 MDSLRKSFKQIGSQVHT-KISAGDRVGAVGVEESKPILSDLDD--MKSIDSSGRKDYTSR 260
MD KSFK S + K+S D A E PILSD M DSS RK+ +
Sbjct: 1 MDFSLKSFKSHPSYKYVRKLSGAD---ATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVK 57
Query: 261 DLELXXXXXXE---VNHETKIWRGSSYEFWSD---------GGSKVGTSNGFDFRXXXXX 404
E VN IWR SSY+FW+D GG+K T++ F+FR
Sbjct: 58 VDEADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAK--TTDDFEFRQHRKD 115
Query: 405 XXXXXXADPPSKLIGQFLHKQKASGDFSLDMDLEMEELSVDTHDGGAGLRTITETPS--T 578
DPPSKLIGQFLHKQKASG+ SLDMD+EM EL D L T+ E+P +
Sbjct: 116 VE-----DPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDK----TPLSTVAESPMRRS 166
Query: 579 PKEIRVSFQD----REEEALPSREDDGIXXXXXXXXXXXXXXXXXXXEGRGDRG--EILR 740
+E++VSF+ E +++ R D D G E+LR
Sbjct: 167 SRELKVSFESISEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLR 226
Query: 741 CTXXXXXXXXXXX-----FLRTKTTKSRLMDPPTPRDDAQDKTSGKPLKSRQLKSGFMGS 905
C+ LR KT KSRL+DPP + QD+ SG+ KS Q++SG +
Sbjct: 227 CSSNSFFQRDVSFQRKSSLLRAKT-KSRLLDPP----EHQDRRSGRVPKSGQVRSGLISK 281
Query: 906 TIDEVEEDPFADEDLPEEFKKGKFNTLTVCQWVSLILILAAFACSVSIPFXXXXXXXXXX 1085
+DE ++DPF +EDLP+E+KK LT+ QW SLILI+AA C+++I +
Sbjct: 282 ALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLE 341
Query: 1086 XXXXXXXXXXXFCGRLLSGWGIRLVVFFIERNFLMRKRVLYFVYGVRKAVQNCLWLGLVL 1265
CGRL+SGWGIR++VFFIERNFL+RKRVLYFVYGVRKAVQNCLWLGLVL
Sbjct: 342 VWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVL 401
Query: 1266 LAWHYILDAKVKNEIKSSPLPFFVTKVLVCLLVAT 1370
+AW+++ D KV+ E+KS+ L +VTKVLVCLLV+T
Sbjct: 402 IAWNFLFDDKVQREVKSNALE-YVTKVLVCLLVST 435
>emb|CAN67321.1| hypothetical protein VITISV_039348 [Vitis vinifera]
Length = 922
Score = 272 bits (696), Expect = 1e-70
Identities = 194/459 (42%), Positives = 240/459 (52%), Gaps = 34/459 (7%)
Frame = +3
Query: 90 MDSLRKSFKQIGSQVHTKISAGDRVGAVGVEESKPILS--DLDDMKSIDSS--GRKDY-- 251
MDSL+KS K GS +TKISAG ++G E +PIL + D +S DS RK++
Sbjct: 1 MDSLKKSIKSHGSYKNTKISAGGPDDSIG--EQQPILMHHNGDRTRSGDSGEVDRKEFIV 58
Query: 252 -------------------TSRDLELXXXXXXEVNHETKIWRGSSYEFWSDGGSKVGTSN 374
S + +KIWR SYEFW DGG S
Sbjct: 59 KIDGEDEGGRGRSSSGGGGVSGSSGSRGSSGSRGSGASKIWREPSYEFWRDGGEIERKSG 118
Query: 375 G-FDFRXXXXXXXXXXXADPPSKLIGQFLHKQKASGDFSLDMDLEMEELSVDTHDGGAGL 551
G F F+ DPPSKLIGQFLHKQKASGDFSLDMD+EMEEL + +
Sbjct: 119 GSFQFQQPLATE------DPPSKLIGQFLHKQKASGDFSLDMDMEMEELRDEKSEPPMPE 172
Query: 552 RTITETPSTPKEIRVSFQDREEEALPSREDD---GIXXXXXXXXXXXXXXXXXXXEGRGD 722
+ S+ +E++VSFQ + A R +
Sbjct: 173 SNMHPMMSS-REMKVSFQPQTTGADEMRSETVRRSYIDKEGSDKDGSDEDDIKRDNCDNP 231
Query: 723 RGEILRCTXXXXXXXXXXXFLRTKTTKSRLMDPPTPRDDAQDKT-----SGKPLKSRQLK 887
GE+L CT LR KT KSRL D R + K+ SG KS LK
Sbjct: 232 DGEVLMCTSNMEFQRKST-LLRNKT-KSRLAD----RTEYVMKSGLVPKSGLLPKSGMLK 285
Query: 888 SGFMGSTIDEVEEDPFADEDLPEEFKKGKFNTLTVCQWVSLILILAAFACSVSIPFXXXX 1067
SG +G + +E EEDPF +DLPEEFK+ F+ T+ QW+ LIL++A CS++IP
Sbjct: 286 SGLLGKS-EEDEEDPFFVDDLPEEFKRSNFSFWTILQWLILILLVAVLVCSLTIPLFKDR 344
Query: 1068 XXXXXXXXXXXXXXXXXFCGRLLSGWGIRLVVFFIERNFLMRKRVLYFVYGVRKAVQNCL 1247
CGRL+SGWGIRLVVFFIERNFL+RKRVLYFVYG+RKAVQNCL
Sbjct: 345 ILWKLRLWRWEVMVLVLICGRLVSGWGIRLVVFFIERNFLLRKRVLYFVYGLRKAVQNCL 404
Query: 1248 WLGLVLLAWHYILDAKVKNEIKSSPLPFFVTKVLVCLLV 1364
WLGLVL+AWH + D KV+ E K+ L +VTK+LVCLLV
Sbjct: 405 WLGLVLIAWHIMFDKKVERETKNDSLK-YVTKILVCLLV 442
>ref|XP_002889201.1| mechanosensitive ion channel domain-containing protein [Arabidopsis
lyrata subsp. lyrata] gi|297335042|gb|EFH65460.1|
mechanosensitive ion channel domain-containing protein
[Arabidopsis lyrata subsp. lyrata]
Length = 857
Score = 256 bits (653), Expect = 1e-65
Identities = 154/378 (40%), Positives = 203/378 (53%), Gaps = 26/378 (6%)
Frame = +3
Query: 309 KIWRGSSYEFWSDGGSKVGTSN--------------------GFDFRXXXXXXXXXXXAD 428
+IWR SY+FW+DG + + GF+FR D
Sbjct: 29 RIWRDGSYDFWTDGEGNLNKGHNAAAVDSDRSAATTEEQQDEGFEFRRGE---------D 79
Query: 429 PPSKLIGQFLHKQKASGDFSLDMDLEMEELSVDTHDGGAGLRTITETPSTPKEIRVSFQD 608
PP+KLIGQFLHKQ+ASG+ LDMDL M+EL GL ++E+P++ K
Sbjct: 80 PPTKLIGQFLHKQQASGEICLDMDLGMDELQ------SRGLTPVSESPTSAK-------- 125
Query: 609 REEEALPSREDDGIXXXXXXXXXXXXXXXXXXXEGRGDRGEILRCTXXXXXXXXXXXFLR 788
+P++ D + D GE+++C+ L+
Sbjct: 126 -----VPTKPPDPVGRRDSRSN-----------NNNNDDGEVVKCSGNAPIQRSSSNLLK 169
Query: 789 TKTTKSRLMDPPTPRDDAQ--DKTSGKPLKSRQLKSGFMGSTI----DEVEEDPFADEDL 950
+T KSRL DPPTP+ Q D SG+ KS Q+KSGF G + +E E+DPFA EDL
Sbjct: 170 MRT-KSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKNQGEEEEDDPFAAEDL 228
Query: 951 PEEFKKGKFNTLTVCQWVSLILILAAFACSVSIPFXXXXXXXXXXXXXXXXXXXXXFCGR 1130
PEE++K K + V +W+SLILI+A F C+++IP CGR
Sbjct: 229 PEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRRKKLWELQLWKWESMVLVLICGR 288
Query: 1131 LLSGWGIRLVVFFIERNFLMRKRVLYFVYGVRKAVQNCLWLGLVLLAWHYILDAKVKNEI 1310
L+S W +++VVFFIERNFL+RKRVLYFVYGVRKAVQNCLWLGLVLLAWH++ D KV
Sbjct: 289 LVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAA 348
Query: 1311 KSSPLPFFVTKVLVCLLV 1364
+ L VTK+ VCLLV
Sbjct: 349 NTKALR-VVTKIFVCLLV 365
>ref|NP_177982.1| mechanosensitive channel of small conductance-like 6 [Arabidopsis
thaliana] gi|75213461|sp|Q9SYM1.1|MSL6_ARATH RecName:
Full=Mechanosensitive ion channel protein 6; AltName:
Full=Mechanosensitive channel of small conductance-like
6; AltName: Full=MscS-Like protein 6
gi|4836872|gb|AAD30575.1|AC007260_6 Hypothetical protein
[Arabidopsis thaliana] gi|332198006|gb|AEE36127.1|
mechanosensitive channel of small conductance-like 6
[Arabidopsis thaliana]
Length = 856
Score = 252 bits (644), Expect = 1e-64
Identities = 156/379 (41%), Positives = 199/379 (52%), Gaps = 27/379 (7%)
Frame = +3
Query: 309 KIWRGSSYEFWSDGGSKVGTSN---------------------GFDFRXXXXXXXXXXXA 425
KIWR SY+FW+DG + + GF+FR
Sbjct: 32 KIWRDGSYDFWTDGEGNLNKGHNAAAVDSDRSAATTGEQQKDEGFEFRRGE--------- 82
Query: 426 DPPSKLIGQFLHKQKASGDFSLDMDLEMEELSVDTHDGGAGLRTITETPSTPKEIRVSFQ 605
DPP+KLIGQFLHKQ+ASG+ LDMDL M+EL GL ++E+P RVS +
Sbjct: 83 DPPTKLIGQFLHKQQASGEICLDMDLGMDELQ------SRGLTPVSESP------RVSTK 130
Query: 606 DREEEALPSREDDGIXXXXXXXXXXXXXXXXXXXEGRGDRGEILRCTXXXXXXXXXXXFL 785
SR + D GE+++C+ L
Sbjct: 131 RDPVGRRDSRSN----------------------TNNNDDGEVVKCSGNNAPIQRSSSTL 168
Query: 786 RTKTTKSRLMDPPTPRDDAQ--DKTSGKPLKSRQLKSGFMGSTI----DEVEEDPFADED 947
T+SRL DPPTP+ Q D SG+ KS Q+KSGF G + +E E+DPFA ED
Sbjct: 169 LKMRTRSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAED 228
Query: 948 LPEEFKKGKFNTLTVCQWVSLILILAAFACSVSIPFXXXXXXXXXXXXXXXXXXXXXFCG 1127
LPEE++K K + V +W+SLILI+A F C+++IP CG
Sbjct: 229 LPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICG 288
Query: 1128 RLLSGWGIRLVVFFIERNFLMRKRVLYFVYGVRKAVQNCLWLGLVLLAWHYILDAKVKNE 1307
RL+S W +++VVFFIERNFL+RKRVLYFVYGVRKAVQNCLWLGLVLLAWH++ D KV
Sbjct: 289 RLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKA 348
Query: 1308 IKSSPLPFFVTKVLVCLLV 1364
+ L VTK+ VCLLV
Sbjct: 349 ANTKALR-VVTKIFVCLLV 366