BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00031985
         (451 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       153   1e-35
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   152   3e-35
dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       150   1e-34
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   149   2e-34
dbj|BAG75115.1| cytochrome P450 [Eschscholzia californica]            143   1e-32

>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  153 bits (387), Expect = 1e-35
 Identities = 79/150 (52%), Positives = 106/150 (70%), Gaps = 1/150 (0%)
 Frame = -3

Query: 449 AQYIPGLSIPFKEASKVKHSIEELIRPYISNHMSFLPVCTNNCYLHFLLSQNFSEEVVIF 270
           A+Y+P      +EA   K+ IEEL+RP +S+          N YL+FLLSQN SEEV IF
Sbjct: 247 AKYLPSQRKATREAKLGKYRIEELVRPLLSSKPP------TNAYLYFLLSQNLSEEVTIF 300

Query: 269 NVFELFLFAVDSIATTTAWALAFLIHDQQIQQKLYEEVHREMGS-EAVKIDKVNKLQYLH 93
            +FELFL  VDS ++TT WALA++I +Q+ Q+KLY+++   +G  + VKI+ VNKL+YL 
Sbjct: 301 CIFELFLLGVDSASSTTTWALAYMIREQEAQEKLYQDIRMTIGDVDLVKIEDVNKLKYLQ 360

Query: 92  AVVKETTRMRPIAPLAVPHRAATDSKLMGT 3
           +VVKET RM+PIAPLA+PH A  D+ LMGT
Sbjct: 361 SVVKETMRMKPIAPLALPHNAGKDTTLMGT 390


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 gi|15822820|dbj|BAB68769.1|
           methylenedioxy bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  152 bits (384), Expect = 3e-35
 Identities = 78/150 (52%), Positives = 106/150 (70%), Gaps = 1/150 (0%)
 Frame = -3

Query: 449 AQYIPGLSIPFKEASKVKHSIEELIRPYISNHMSFLPVCTNNCYLHFLLSQNFSEEVVIF 270
           A+Y+P      +EA  VK  +EEL+RP +S+          N YL+FLLSQNF EEV+IF
Sbjct: 231 AKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPP------TNSYLYFLLSQNFEEEVIIF 284

Query: 269 NVFELFLFAVDSIATTTAWALAFLIHDQQIQQKLYEEVHREMGS-EAVKIDKVNKLQYLH 93
            +FEL+L  VDS ++TT WALA+LI +Q  Q+KLY+++   +G  + VKI+ VNKL+YL 
Sbjct: 285 CIFELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQ 344

Query: 92  AVVKETTRMRPIAPLAVPHRAATDSKLMGT 3
            VVKET RM+PIAPLA+PH+ A ++ LMGT
Sbjct: 345 GVVKETMRMKPIAPLAIPHKTAKETTLMGT 374


>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  150 bits (379), Expect = 1e-34
 Identities = 76/150 (50%), Positives = 107/150 (71%), Gaps = 1/150 (0%)
 Frame = -3

Query: 449 AQYIPGLSIPFKEASKVKHSIEELIRPYISNHMSFLPVCTNNCYLHFLLSQNFSEEVVIF 270
           A+Y+P      +EA   K+ IEEL+RP +S++         N YL+FL SQN+S+E++IF
Sbjct: 231 AKYLPSQKKVAREAYLAKYRIEELMRPLLSSNPP------TNSYLYFLQSQNYSKEIIIF 284

Query: 269 NVFELFLFAVDSIATTTAWALAFLIHDQQIQQKLYEEVHREMGS-EAVKIDKVNKLQYLH 93
             FELFL  VDS ++T  WALA+LIH+Q +Q+KL E++   +G  + VKI+ ++KL+YL 
Sbjct: 285 CTFELFLLGVDSTSSTATWALAYLIHEQAVQEKLNEDIRMTIGDVDLVKIEDLSKLKYLQ 344

Query: 92  AVVKETTRMRPIAPLAVPHRAATDSKLMGT 3
           AVVKET RM+PIAPLA+PH+ A D+ LMGT
Sbjct: 345 AVVKETMRMKPIAPLAIPHKTANDTTLMGT 374


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  149 bits (377), Expect = 2e-34
 Identities = 77/150 (51%), Positives = 107/150 (71%), Gaps = 1/150 (0%)
 Frame = -3

Query: 449 AQYIPGLSIPFKEASKVKHSIEELIRPYISNHMSFLPVCTNNCYLHFLLSQNFSEEVVIF 270
           A+Y+P      +EA  VK  +E+L+ P +S++         N YL+FLLSQ FSEEV+IF
Sbjct: 232 AKYLPSHKKAEREAYLVKCRVEDLVSPLLSSNPP------TNSYLYFLLSQRFSEEVIIF 285

Query: 269 NVFELFLFAVDSIATTTAWALAFLIHDQQIQQKLYEEVHREMGS-EAVKIDKVNKLQYLH 93
            +FEL+L  VDS ++TT WALA+LI +Q IQ+KLY++    +G  + VKI+ V+KL+YL 
Sbjct: 286 CIFELYLLGVDSTSSTTTWALAYLIREQAIQEKLYQDTRMTLGDVDLVKIEDVSKLKYLQ 345

Query: 92  AVVKETTRMRPIAPLAVPHRAATDSKLMGT 3
           AVVKET RM+PIAPLA+PH+ A D+ LMG+
Sbjct: 346 AVVKETMRMKPIAPLAIPHKTAKDTTLMGS 375


>dbj|BAG75115.1| cytochrome P450 [Eschscholzia californica]
          Length = 494

 Score =  143 bits (361), Expect = 1e-32
 Identities = 74/151 (49%), Positives = 103/151 (68%), Gaps = 3/151 (1%)
 Frame = -3

Query: 449 AQYIPGLSIPFKEASKVKHSIEELIRPYISNHMSFLPVCTNNCYLHFLLSQNFSEEVVIF 270
           A+Y+P      +   + K  +++LIRPYIS  +S  P    N YLHFL SQN+ EEVVIF
Sbjct: 235 AKYLPSHRKAVRHFEQTKQIVKKLIRPYIS--VSPPP----NTYLHFLQSQNYDEEVVIF 288

Query: 269 NVFELFLFAVDSIATTTAWALAFLIHDQQIQQKLYEEVH---REMGSEAVKIDKVNKLQY 99
            +FE +L  VDS ++TT WALAFLI +  +Q+KLY+E+     +   E +K++ +NKLQY
Sbjct: 289 AIFEAYLLGVDSTSSTTTWALAFLIREPNVQEKLYQELKNFAEQNDREIMKVEDINKLQY 348

Query: 98  LHAVVKETTRMRPIAPLAVPHRAATDSKLMG 6
           L AV KET RM+P+APLA+PH+A  ++ LMG
Sbjct: 349 LQAVTKETMRMKPVAPLAIPHKACRETSLMG 379