BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00023991
         (923 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI24150.3| unnamed protein product [Vitis vinifera]              375   e-102
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop...   364   2e-98
gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]             358   1e-96
ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precurso...   349   5e-94
ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa] g...   345   9e-93

>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
          Length = 826

 Score =  375 bits (964), Expect = e-102
 Identities = 183/309 (59%), Positives = 238/309 (77%), Gaps = 4/309 (1%)
 Frame = -3

Query: 921  SPSTTAAFVIQHHDANCFEYLRSLLEKFGNAVPTVYPINIYIRLSMVDRLERLGIDRHFK 742
            SPS TAA +    +A C  YLRSLLEKFGNAVPTVYP+++Y RL +VD LERLGIDR+F+
Sbjct: 275  SPSATAAALSHLQNAGCLNYLRSLLEKFGNAVPTVYPLDLYARLCLVDNLERLGIDRYFR 334

Query: 741  NEIKDVLDDTYRCWLHKDEEIFLDRETCALAFRLLQTNGYELFSDALAEFKEEDYYVNSV 562
             EI+ VLD+TYRCWL ++EEIF DR TCA+AFR+L+ NGY++ S  LA+F E+D Y    
Sbjct: 335  MEIRSVLDETYRCWLQREEEIFADRATCAIAFRILRLNGYDISSVPLAQFAEDDQYFK-F 393

Query: 561  GMNLKDMYTVLELYKASEIMILPDDQVLETLHSWTSHFLKQGILNGAIRDEKCYK----E 394
            G + KD+   LEL++ASE++I PD+ VLE  +SW+SHFL+QG+ N +I  ++  K    E
Sbjct: 394  GQDFKDLGAALELFRASEMIIHPDEVVLEKQNSWSSHFLRQGLSNSSIHADRLNKYIAQE 453

Query: 393  VKHALRFNFDANVERLHNRRNIECYDVDDIRILKASYRCSNIYNEDFLKFAVQDYNSCQA 214
            V+ ALRF + AN++R+ NRR+IE Y+VDD RILK +YR S++ N+DFLK AV+D+N CQ+
Sbjct: 454  VEDALRFPYYANLDRIANRRSIEHYNVDDTRILKTAYRSSHVCNKDFLKLAVEDFNFCQS 513

Query: 213  IQQKELENLERWIEENRLDQLKFARQKLTYLYFSVACILFAPELSDARMSWTKNAFLVIL 34
            I Q EL+ LERWI ENRLD+LKFARQKL Y YFS A  +F+PE SDAR+SW KN+ L  +
Sbjct: 514  IHQNELKQLERWIIENRLDKLKFARQKLAYCYFSAAATIFSPEQSDARLSWAKNSVLTTV 573

Query: 33   VDDFFDVGG 7
            VDDFFD+GG
Sbjct: 574  VDDFFDIGG 582


>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
            vinifera]
          Length = 793

 Score =  364 bits (934), Expect = 2e-98
 Identities = 179/309 (57%), Positives = 234/309 (75%), Gaps = 4/309 (1%)
 Frame = -3

Query: 921  SPSTTAAFVIQHHDANCFEYLRSLLEKFGNAVPTVYPINIYIRLSMVDRLERLGIDRHFK 742
            SPS TAA +    +A C  YLRSLLEKFGNAVPTVYP+++Y RL +VD LERLGIDR+F+
Sbjct: 250  SPSATAAALSHLQNAGCLNYLRSLLEKFGNAVPTVYPLDLYARLCLVDNLERLGIDRYFR 309

Query: 741  NEIKDVLDDTYRCWLHKDEEIFLDRETCALAFRLLQTNGYELFSDALAEFKEEDYYVNSV 562
             EI+ VLD+TYRCWL ++EEIF DR TCA+AFR+L+ NGY++ SD       + Y+    
Sbjct: 310  MEIRSVLDETYRCWLQREEEIFADRATCAIAFRILRLNGYDISSD-------DQYF--KF 360

Query: 561  GMNLKDMYTVLELYKASEIMILPDDQVLETLHSWTSHFLKQGILNGAIRDEKCYK----E 394
            G + KD+   LEL++ASE++I PD+ VLE  +SW+SHFL+QG+ N +I  ++  K    E
Sbjct: 361  GQDFKDLGAALELFRASEMIIHPDEVVLEKQNSWSSHFLRQGLSNSSIHADRLNKYIAQE 420

Query: 393  VKHALRFNFDANVERLHNRRNIECYDVDDIRILKASYRCSNIYNEDFLKFAVQDYNSCQA 214
            V+ ALRF + AN++R+ NRR+IE Y+VDD RILK +YR S++ N+DFLK AV+D+N CQ+
Sbjct: 421  VEDALRFPYYANLDRIANRRSIEHYNVDDTRILKTAYRSSHVCNKDFLKLAVEDFNFCQS 480

Query: 213  IQQKELENLERWIEENRLDQLKFARQKLTYLYFSVACILFAPELSDARMSWTKNAFLVIL 34
            I Q EL+ LERWI ENRLD+LKFARQKL Y YFS A  +F+PE SDAR+SW KN+ L  +
Sbjct: 481  IHQNELKQLERWIIENRLDKLKFARQKLAYCYFSAAATIFSPEQSDARLSWAKNSVLTTV 540

Query: 33   VDDFFDVGG 7
            VDDFFD+GG
Sbjct: 541  VDDFFDIGG 549


>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
          Length = 784

 Score =  358 bits (918), Expect = 1e-96
 Identities = 180/309 (58%), Positives = 232/309 (75%), Gaps = 4/309 (1%)
 Frame = -3

Query: 921  SPSTTAAFVIQHHDANCFEYLRSLLEKFGNAVPTVYPINIYIRLSMVDRLERLGIDRHFK 742
            SPSTTAA      ++ C  YL SLL++FGNAVPTVYP++IY RL MVD LERLGIDRHF+
Sbjct: 233  SPSTTAAAFTYLKNSGCLSYLHSLLDRFGNAVPTVYPLDIYTRLCMVDSLERLGIDRHFR 292

Query: 741  NEIKDVLDDTYRCWLHKDEEIFLDRETCALAFRLLQTNGYELFSDALAEFKEEDYYVNSV 562
             EIK VLD+TYRCWL  +EEIFLD  TCA+AFR+L+ NG+++ SD   +  E D++ +S+
Sbjct: 293  KEIKSVLDETYRCWLQGEEEIFLDTATCAMAFRILRVNGFDISSDPFTQLSE-DHFSSSL 351

Query: 561  GMNLKDMYTVLELYKASEIMILPDDQVLETLHSWTSHFLKQGILNGAIRDEKCYK----E 394
            G  +KD+ +VLEL++AS+I+I PD+ VLE  + WTS FL Q + NG+I  +   K    E
Sbjct: 352  GGYMKDIGSVLELFRASQIIIHPDEFVLEKQNFWTSQFLIQELSNGSIHADGLNKYVSQE 411

Query: 393  VKHALRFNFDANVERLHNRRNIECYDVDDIRILKASYRCSNIYNEDFLKFAVQDYNSCQA 214
            V  AL+F + A++ERL NRR IE Y+ ++ R+LK +Y  SNI NEDFL  AV+D+N CQ+
Sbjct: 412  VDDALKFPYHASLERLSNRRAIENYNKNNTRVLKTAYSSSNIGNEDFLNLAVEDFNICQS 471

Query: 213  IQQKELENLERWIEENRLDQLKFARQKLTYLYFSVACILFAPELSDARMSWTKNAFLVIL 34
            IQ++EL++L RWI ENRLD+LKFARQKL Y YFS A  LF+PELSDAR+SW KN  L  +
Sbjct: 472  IQREELKDLARWITENRLDKLKFARQKLAYCYFSAAATLFSPELSDARISWAKNGVLTTV 531

Query: 33   VDDFFDVGG 7
            VDDFFDVGG
Sbjct: 532  VDDFFDVGG 540


>ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
            communis] gi|223526405|gb|EEF28689.1| Ent-kaurene
            synthase B, chloroplast precursor, putative [Ricinus
            communis]
          Length = 808

 Score =  349 bits (896), Expect = 5e-94
 Identities = 180/309 (58%), Positives = 233/309 (75%), Gaps = 4/309 (1%)
 Frame = -3

Query: 921  SPSTTAAFVIQHHDANCFEYLRSLLEKFGNAVPTVYPINIYIRLSMVDRLERLGIDRHFK 742
            SPSTTAA      +A+C +YL+S+L+K+GNAVPT+YP+++Y RL MVD LERLGIDRHF+
Sbjct: 188  SPSTTAAAFSHLRNADCLQYLQSVLQKYGNAVPTIYPLDVYSRLLMVDILERLGIDRHFR 247

Query: 741  NEIKDVLDDTYRCWLHKDEEIFLDRETCALAFRLLQTNGYELFSDALAEFKEEDYYVNSV 562
             EIK VL++TYR WL  +EEIFLD  TCA+AFR+L+ NGY++ SD   +F E D++ +S+
Sbjct: 248  KEIKLVLEETYRYWLQGNEEIFLDCITCAMAFRILRVNGYDVSSDVFTQFTE-DHFFDSL 306

Query: 561  GMNLKDMYTVLELYKASEIMILPDDQVLETLHSWTSHFLKQGILNG---AIRDEKCYKEV 391
            G  LKD  TVLELY+AS+I+  PD+ +LE  +SWT+HFL++ + +G   A    +C  EV
Sbjct: 307  GGYLKDTRTVLELYRASQILY-PDEPLLEKQNSWTNHFLEKCLSSGSSYADGPRECITEV 365

Query: 390  KH-ALRFNFDANVERLHNRRNIECYDVDDIRILKASYRCSNIYNEDFLKFAVQDYNSCQA 214
             H AL   + A++ERL NRR+IE Y+VD+ RILKASYRC N  N+ FLK AV+D+N CQ 
Sbjct: 366  VHNALNCPYYADLERLTNRRSIENYNVDETRILKASYRCLNTGNQHFLKLAVEDFNLCQL 425

Query: 213  IQQKELENLERWIEENRLDQLKFARQKLTYLYFSVACILFAPELSDARMSWTKNAFLVIL 34
            I Q+EL+ L RW+ E RL++LKFARQKL Y YFS A  LFAPELSDAR+SW KN  L  +
Sbjct: 426  IHQEELQQLGRWVVEKRLNKLKFARQKLGYCYFSAAATLFAPELSDARLSWAKNGVLTTV 485

Query: 33   VDDFFDVGG 7
            VDDFFDVGG
Sbjct: 486  VDDFFDVGG 494


>ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa]
            gi|222851106|gb|EEE88653.1| ent-kaurene synthase [Populus
            trichocarpa]
          Length = 743

 Score =  345 bits (885), Expect = 9e-93
 Identities = 180/309 (58%), Positives = 223/309 (72%), Gaps = 4/309 (1%)
 Frame = -3

Query: 921  SPSTTAAFVIQHHDANCFEYLRSLLEKFGNAVPTVYPINIYIRLSMVDRLERLGIDRHFK 742
            SPSTTAA  I   DA C  Y+RSLL+KFGNAVPT+YP++IY RLSMVD LERLGIDRHF+
Sbjct: 200  SPSTTAAAFIHIQDAECLHYIRSLLQKFGNAVPTIYPLDIYARLSMVDALERLGIDRHFR 259

Query: 741  NEIKDVLDDTYRCWLHKDEEIFLDRETCALAFRLLQTNGYELFSDALAEFKEEDYYVNSV 562
             E K VLD+TYR WL  +EEIF D  TCALAFR+L+ NGY++          ED++ NS+
Sbjct: 260  KERKFVLDETYRFWLQGEEEIFSDNATCALAFRILRLNGYDV--------SLEDHFSNSL 311

Query: 561  GMNLKDMYTVLELYKASEIMILPDDQVLETLHSWTSHFLKQGILNGAIRDEKCYK----E 394
            G  LKD    LELY+A ++   PD+ +LE  +S TS+FLKQG+ N ++  ++  K    E
Sbjct: 312  GGYLKDSGAALELYRALQLSY-PDESLLEKQNSRTSYFLKQGLSNVSLCGDRLRKNIIGE 370

Query: 393  VKHALRFNFDANVERLHNRRNIECYDVDDIRILKASYRCSNIYNEDFLKFAVQDYNSCQA 214
            V  AL F+  AN++RL  RR I+ Y  DD RILK SYRCS I N+DFLK AV+D+N CQ+
Sbjct: 371  VHDALNFSDHANLQRLAIRRRIKHYATDDTRILKTSYRCSTIGNQDFLKLAVEDFNICQS 430

Query: 213  IQQKELENLERWIEENRLDQLKFARQKLTYLYFSVACILFAPELSDARMSWTKNAFLVIL 34
            IQ++E +++ERW+ E RLD+LKFARQK  Y YFS A  LFAPELSDARMSW KN  L  +
Sbjct: 431  IQREEFKHIERWVVERRLDKLKFARQKEAYCYFSAAATLFAPELSDARMSWAKNGVLTTV 490

Query: 33   VDDFFDVGG 7
            VDDFFDVGG
Sbjct: 491  VDDFFDVGG 499