BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00018507
         (2348 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002280913.1| PREDICTED: protein BONZAI 1 [Vitis vinifera]...   865   0.0  
ref|XP_002511086.1| copine, putative [Ricinus communis] gi|22355...   861   0.0  
ref|XP_003532331.1| PREDICTED: protein BONZAI 1-like [Glycine max]    852   0.0  
ref|XP_002321753.1| predicted protein [Populus trichocarpa] gi|2...   852   0.0  
ref|XP_002318175.1| predicted protein [Populus trichocarpa] gi|2...   849   0.0  

>ref|XP_002280913.1| PREDICTED: protein BONZAI 1 [Vitis vinifera]
            gi|297734511|emb|CBI15758.3| unnamed protein product
            [Vitis vinifera]
          Length = 576

 Score =  865 bits (2234), Expect = 0.0
 Identities = 439/582 (75%), Positives = 494/582 (84%), Gaps = 1/582 (0%)
 Frame = +1

Query: 10   MGNCCSDNPXXXXXXXXXXXXYNNPASGATANES-DFFLKSRGYQSLFTQIELSFSASNL 186
            MGNCCSD+                 AS    N++ D FLKSRGY+ LF QIELS SASNL
Sbjct: 1    MGNCCSDDGGGRSAVG------GTSASQHDHNDAVDLFLKSRGYRGLFVQIELSLSASNL 54

Query: 187  QDRDILSKNDPMAVFYAKGRDGKLEELGRTEVVLNSLNPTWITKYVITYQFEVVQHMVFH 366
            +DRD+LSK+DPMAV Y K +DG LEELGRTEVVLNSLNP W TK +I YQFEVVQ +VF 
Sbjct: 55   RDRDVLSKSDPMAVVYTKSKDGALEELGRTEVVLNSLNPMWFTKLIINYQFEVVQTLVFR 114

Query: 367  VYDVDTQFHNVPVKMLKLDEQQFLGEATCALSEIVTKPSRSLTLDIICREESNRSDRPRR 546
            VYDVDTQFHNV VKMLKLDEQQFLGEATC+LSEIV+K +RSLTLD++ REES +S R + 
Sbjct: 115  VYDVDTQFHNVDVKMLKLDEQQFLGEATCSLSEIVSKSNRSLTLDLVHREESIKSTRSQN 174

Query: 547  LGELTVHAEECIGSKTMTEMIFRCSDLEYKDLFSKSDPFLVISRTVESGGTIPVCKTEVK 726
            LGELTVHAEE   SK   E+IFRCSDLEYKDLFS+SDPFLVIS+ VE G +IP+CKTEV 
Sbjct: 175  LGELTVHAEELSSSKIAMEIIFRCSDLEYKDLFSRSDPFLVISKIVEGGISIPICKTEVI 234

Query: 727  KNDLNPTWKPVCLNIQQVGSKDSPLHIECLNFNSNGRHDLIGKVQKSLVDLEKLQHNEQG 906
            KNDL P WK V LNIQQVGSKD+ L IEC NFNSNG+HDLIGK+QKSL DLEKL  + QG
Sbjct: 235  KNDLKPKWKSVFLNIQQVGSKDNLLTIECFNFNSNGKHDLIGKIQKSLADLEKLHSSGQG 294

Query: 907  ESMFLPTVVGHSYQNKVLKSRIFVDKFSECVQNTFLDYIAGGCELNFMVAVDFTASNGNP 1086
            E+ FLPT VG+++ NKVLKS++FV+ FSE V++TFLDY+AGGCELNFMVA+DFTASNGNP
Sbjct: 295  ENFFLPTAVGNNHHNKVLKSQLFVEYFSETVEHTFLDYLAGGCELNFMVAIDFTASNGNP 354

Query: 1087 RLPDSLHYIDPSGRPNAYQKAILEVGGVLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNG 1266
            RLPDSLHYIDPSGRPNAYQ+AILEVG VLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNG
Sbjct: 355  RLPDSLHYIDPSGRPNAYQRAILEVGEVLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNG 414

Query: 1267 SSYHSEVEGIQGIMAAYASAIPNVSLAGPTLFGPVINNASIIASHALANNLQKYFVLLII 1446
            SS + EV+GIQGIM AY SA+ NVSLAGPTLFGPVI++A+ IAS +L N  QKY+VLLII
Sbjct: 415  SSNYCEVDGIQGIMMAYTSALFNVSLAGPTLFGPVISSAAQIASQSLINGRQKYYVLLII 474

Query: 1447 TDGVITDLQETKDALVKASDLPLSILIVGVGGADFKEMEVLDADKGDRLESSAGRIASRN 1626
            TDGVITDLQETKDALVKASDLPLSI I+GVGGADFKEMEVLDADKG+RLESS GR+ASR+
Sbjct: 475  TDGVITDLQETKDALVKASDLPLSIFILGVGGADFKEMEVLDADKGERLESSNGRVASRD 534

Query: 1627 IVQFVPFRDVQSGEILVLQSLLAELPVQFLTYMRSRDIRPQT 1752
            IVQFVPFRDV SGEI V+Q+LLAELP  FLTYMR+R+I P +
Sbjct: 535  IVQFVPFRDVHSGEISVVQALLAELPTHFLTYMRTRNILPNS 576


>ref|XP_002511086.1| copine, putative [Ricinus communis] gi|223550201|gb|EEF51688.1|
            copine, putative [Ricinus communis]
          Length = 577

 Score =  861 bits (2225), Expect = 0.0
 Identities = 425/580 (73%), Positives = 498/580 (85%), Gaps = 1/580 (0%)
 Frame = +1

Query: 10   MGNCCSDNPXXXXXXXXXXXXYNNPASGATANES-DFFLKSRGYQSLFTQIELSFSASNL 186
            MGNCCSD                  ++G T+N++ DFFL+SRG+  +F+QIELSFSA+NL
Sbjct: 1    MGNCCSDEAGGRAAVGGTLAA----STGGTSNDAVDFFLRSRGFHGIFSQIELSFSATNL 56

Query: 187  QDRDILSKNDPMAVFYAKGRDGKLEELGRTEVVLNSLNPTWITKYVITYQFEVVQHMVFH 366
            +DRD+LSK+DPM V Y KGRDG   E+ RTEVVLNSLNPTWI KY IT+QFEVVQ+++F 
Sbjct: 57   RDRDVLSKSDPMVVVYTKGRDGPPIEVFRTEVVLNSLNPTWIMKYSITFQFEVVQNLLFR 116

Query: 367  VYDVDTQFHNVPVKMLKLDEQQFLGEATCALSEIVTKPSRSLTLDIICREESNRSDRPRR 546
            VYD+D+QFHNV VKMLKLDEQQFLGEA+C LSEIVTKP RSLTLD++ +E+S  S  PR 
Sbjct: 117  VYDIDSQFHNVEVKMLKLDEQQFLGEASCVLSEIVTKPLRSLTLDLVHKEDSTLSSHPRG 176

Query: 547  LGELTVHAEECIGSKTMTEMIFRCSDLEYKDLFSKSDPFLVISRTVESGGTIPVCKTEVK 726
             G+L VHAEECI SKT TEMI RCSDLE+KDLFS+ +PFLVIS+TVE+G  IPVC+TEV 
Sbjct: 177  -GQLVVHAEECISSKTTTEMILRCSDLEHKDLFSRINPFLVISKTVENGNPIPVCRTEVL 235

Query: 727  KNDLNPTWKPVCLNIQQVGSKDSPLHIECLNFNSNGRHDLIGKVQKSLVDLEKLQHNEQG 906
            KND NP WKP+ LNIQQVGSKDSPL IEC NFNSNG+HDLIG+++KSL +LEKL+ +   
Sbjct: 236  KNDHNPIWKPIFLNIQQVGSKDSPLVIECYNFNSNGKHDLIGELRKSLAELEKLRSSGGS 295

Query: 907  ESMFLPTVVGHSYQNKVLKSRIFVDKFSECVQNTFLDYIAGGCELNFMVAVDFTASNGNP 1086
            E++FLP  VGH + NK LKS+++V++FSE ++ TFLDY+ GGCELNFMVA+DFTASNGNP
Sbjct: 296  ENLFLPNAVGHDHHNKALKSQLYVERFSESIKYTFLDYLKGGCELNFMVAIDFTASNGNP 355

Query: 1087 RLPDSLHYIDPSGRPNAYQKAILEVGGVLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNG 1266
            RLPDSLHY+DPS RPNAYQ+AI+EVG VLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNG
Sbjct: 356  RLPDSLHYLDPSARPNAYQQAIMEVGEVLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNG 415

Query: 1267 SSYHSEVEGIQGIMAAYASAIPNVSLAGPTLFGPVINNASIIASHALANNLQKYFVLLII 1446
            S+ H EVEGIQGIM AY SA+ NVSLAGPTLFGPVIN A++IAS +LAN  +KYFVLLII
Sbjct: 416  SNNHCEVEGIQGIMMAYTSALFNVSLAGPTLFGPVINTAALIASQSLANGGKKYFVLLII 475

Query: 1447 TDGVITDLQETKDALVKASDLPLSILIVGVGGADFKEMEVLDADKGDRLESSAGRIASRN 1626
            TDGV+TDLQETKDA+VKASDLPLSILIVGVGGADFKEME+LDADKG+RLESSAGR+ASR+
Sbjct: 476  TDGVVTDLQETKDAIVKASDLPLSILIVGVGGADFKEMEILDADKGERLESSAGRVASRD 535

Query: 1627 IVQFVPFRDVQSGEILVLQSLLAELPVQFLTYMRSRDIRP 1746
            IVQF+PFR+VQSG+  V+Q+LLAELP QFLTYMRSRDI+P
Sbjct: 536  IVQFIPFRNVQSGQASVVQALLAELPTQFLTYMRSRDIQP 575


>ref|XP_003532331.1| PREDICTED: protein BONZAI 1-like [Glycine max]
          Length = 580

 Score =  852 bits (2202), Expect = 0.0
 Identities = 425/581 (73%), Positives = 491/581 (84%)
 Frame = +1

Query: 10   MGNCCSDNPXXXXXXXXXXXXYNNPASGATANESDFFLKSRGYQSLFTQIELSFSASNLQ 189
            MGNCCSD              + NPA+ A  +  D FL+SRGY  LF+QIELSFSAS L+
Sbjct: 1    MGNCCSDVAGGQSAIGGTAAGHLNPAN-APNDAVDRFLRSRGYNGLFSQIELSFSASGLR 59

Query: 190  DRDILSKNDPMAVFYAKGRDGKLEELGRTEVVLNSLNPTWITKYVITYQFEVVQHMVFHV 369
            DRD+LSK+DPM V YA+G++  L ELGRTEV+LNSLNPTWITK+ + Y FEVVQ +VF V
Sbjct: 60   DRDVLSKSDPMMVLYARGKNAALTELGRTEVILNSLNPTWITKHTLIYNFEVVQVLVFRV 119

Query: 370  YDVDTQFHNVPVKMLKLDEQQFLGEATCALSEIVTKPSRSLTLDIICREESNRSDRPRRL 549
            YDVDTQFHN+ VKMLKL+EQQ+LGEATCALSEI+TK  RS+TLD+  RE+S RS + +  
Sbjct: 120  YDVDTQFHNIDVKMLKLEEQQYLGEATCALSEIITKFDRSMTLDLHRREDSIRSTQSQNC 179

Query: 550  GELTVHAEECIGSKTMTEMIFRCSDLEYKDLFSKSDPFLVISRTVESGGTIPVCKTEVKK 729
            G+L VHAEEC+ SKT  EMI RCSDLEY+DLFS+SDPFL++S+ VE G  IP+CKTEV K
Sbjct: 180  GKLLVHAEECVSSKTTIEMILRCSDLEYRDLFSRSDPFLLLSKVVEGGSHIPICKTEVIK 239

Query: 730  NDLNPTWKPVCLNIQQVGSKDSPLHIECLNFNSNGRHDLIGKVQKSLVDLEKLQHNEQGE 909
            ND NP WKPV LNIQQVGSK+SPL IEC NFNSNG+HDL+GKVQKSLV+LEKL    QGE
Sbjct: 240  NDHNPIWKPVFLNIQQVGSKESPLIIECYNFNSNGKHDLMGKVQKSLVELEKLYSGGQGE 299

Query: 910  SMFLPTVVGHSYQNKVLKSRIFVDKFSECVQNTFLDYIAGGCELNFMVAVDFTASNGNPR 1089
            +       GH+  NKVLKS++FVDKFSE +Q +FLDY+AGG ELNFMVA+DFTASNGNPR
Sbjct: 300  NFISSAPAGHNSHNKVLKSQLFVDKFSESIQYSFLDYLAGGFELNFMVAIDFTASNGNPR 359

Query: 1090 LPDSLHYIDPSGRPNAYQKAILEVGGVLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNGS 1269
            LPDSLHYIDPSGRPNAYQ+A+LEVG VLQ+YDSDKRFP WGFGARPID PVSHCFNLNGS
Sbjct: 360  LPDSLHYIDPSGRPNAYQRAVLEVGEVLQYYDSDKRFPTWGFGARPIDAPVSHCFNLNGS 419

Query: 1270 SYHSEVEGIQGIMAAYASAIPNVSLAGPTLFGPVINNASIIASHALANNLQKYFVLLIIT 1449
            S+  EVEGIQGIM AY SA+ NVSLAGPTLFGPVI+NA++IAS ++AN  +KYFVLLIIT
Sbjct: 420  SHCCEVEGIQGIMMAYTSALLNVSLAGPTLFGPVISNAALIASQSVANGGRKYFVLLIIT 479

Query: 1450 DGVITDLQETKDALVKASDLPLSILIVGVGGADFKEMEVLDADKGDRLESSAGRIASRNI 1629
            DGV+TDLQETKDALVKASDLPLSILIVGVGGADFKEME+LDADKG+RLESS+GR+ASR+I
Sbjct: 480  DGVVTDLQETKDALVKASDLPLSILIVGVGGADFKEMEILDADKGERLESSSGRVASRDI 539

Query: 1630 VQFVPFRDVQSGEILVLQSLLAELPVQFLTYMRSRDIRPQT 1752
            VQFVPFRDVQSGEI V+Q+LLAELP QFL+YMRSR+I P +
Sbjct: 540  VQFVPFRDVQSGEISVVQALLAELPTQFLSYMRSRNILPNS 580


>ref|XP_002321753.1| predicted protein [Populus trichocarpa] gi|222868749|gb|EEF05880.1|
            predicted protein [Populus trichocarpa]
          Length = 573

 Score =  852 bits (2202), Expect = 0.0
 Identities = 429/582 (73%), Positives = 496/582 (85%), Gaps = 3/582 (0%)
 Frame = +1

Query: 10   MGNCCSDNPXXXXXXXXXXXXYNNPASGATANES--DFFLKSRGYQSLFTQIELSFSASN 183
            MGNCCSD                   SGA A     D FLKSRG  S  +Q+ELSFSA+N
Sbjct: 1    MGNCCSDVAGGRAAVG---------GSGAAAGNDAVDHFLKSRGMYS--SQMELSFSATN 49

Query: 184  LQDRDILSKNDPMAVFYAKGRDGKLEELGRTEVVLNSLNPTWITKYVITYQFEVVQHMVF 363
            L+DRD+LSK+DP+ + Y KG+DG L E+ RTEVVLNSLNPTWITK+VIT+QFE VQ +VF
Sbjct: 50   LRDRDVLSKSDPILIGYTKGKDGTLAEVFRTEVVLNSLNPTWITKHVITFQFEAVQTLVF 109

Query: 364  HVYDVDTQFHNVPVKMLKLDEQQFLGEATCALSEIVTKPSRSLTLDIICREESNRSDRPR 543
            H YDVDTQFHN+ VKMLKL+E QFLGEA+C LSE+VT+PSRSLTLD++ RE+   S  PR
Sbjct: 110  HAYDVDTQFHNIDVKMLKLEELQFLGEASCVLSEVVTRPSRSLTLDLLFREDPTMSGNPR 169

Query: 544  RLGELTVHAEECIGSKTMTEMIFRCSDLEYKDLFSKSDPFLVISRTVESGGTIPVCKTEV 723
              G+L VHAEECI SK+  EM+ RCSDLE+KDLFSKSDPFL+IS+ VE G  IPVCKTEV
Sbjct: 170  HCGQLIVHAEECISSKSTVEMVLRCSDLEHKDLFSKSDPFLLISKIVEVGLPIPVCKTEV 229

Query: 724  KKNDLNPTWKPVCLNIQQVGSKDSPLHIECLNFNSNGRHDLIGKVQKSLVDLEKLQHNEQ 903
             KND NP WKP+ L+IQQVGSK++PL IEC NFNS+G+HDLIGKV+KSLV+LEKL  N +
Sbjct: 230  LKNDHNPVWKPIYLSIQQVGSKENPLMIECFNFNSSGKHDLIGKVKKSLVELEKLHSNGE 289

Query: 904  GESMFLPTVVGHSYQNKVLKSRIFVDKFSECVQNTFLDYIAGGCELNFMVAVDFTASNGN 1083
            GE++FLPT VGH++QNKVLKS++FVDKFS+  Q TFLDY+AGGCELNFMVAVDFTASNGN
Sbjct: 290  GENLFLPTTVGHNHQNKVLKSQLFVDKFSQSSQWTFLDYLAGGCELNFMVAVDFTASNGN 349

Query: 1084 PRLPDSLHYIDPSGRPNAYQKAILEVGGVLQFYDSDKRFPAWGFGARPIDGPVSHCFNLN 1263
            PRLPDSLHY+DPSGR NAYQ+AILEVG VL+FYDSDKRFPAWGFGARPIDGPVSHCFNLN
Sbjct: 350  PRLPDSLHYLDPSGRLNAYQRAILEVGEVLKFYDSDKRFPAWGFGARPIDGPVSHCFNLN 409

Query: 1264 GSSYHSEVEGIQGIMAAYASAIPNVSLAGPTLFGPVINNASIIASHALANNLQKYFVLLI 1443
            GS+ HSEV+GIQGIM AY SA+ NVSLAGPTLFGPVI+NA++IAS + AN  +KYFVLLI
Sbjct: 410  GSNNHSEVDGIQGIMTAYTSALYNVSLAGPTLFGPVISNAALIASQSPANGGKKYFVLLI 469

Query: 1444 ITDGVITDLQETKDALVKASDLPLSILIVGVGGADFKEMEVLDADKGDRLESSAGRIASR 1623
            ITDGV+TDLQETKDA+VKASDLPLSILIVGVGGADFKEME+LDADKG+RLESS+GR+ASR
Sbjct: 470  ITDGVVTDLQETKDAIVKASDLPLSILIVGVGGADFKEMEILDADKGERLESSSGRVASR 529

Query: 1624 NIVQFVPFRDVQSGEILVLQSLLAELPVQFLTYMRSR-DIRP 1746
            +IVQF+ FRDVQSGEI V+Q+LLAELP QFL+YMRSR DI+P
Sbjct: 530  DIVQFMSFRDVQSGEIAVVQALLAELPTQFLSYMRSREDIQP 571


>ref|XP_002318175.1| predicted protein [Populus trichocarpa] gi|222858848|gb|EEE96395.1|
            predicted protein [Populus trichocarpa]
          Length = 576

 Score =  849 bits (2194), Expect = 0.0
 Identities = 425/580 (73%), Positives = 491/580 (84%), Gaps = 1/580 (0%)
 Frame = +1

Query: 10   MGNCCSDNPXXXXXXXXXXXXYNNPASGATANES-DFFLKSRGYQSLFTQIELSFSASNL 186
            MGNC SD                  AS A  N++ D FLKSRG     +QIELSFSA+N+
Sbjct: 1    MGNCFSDVAGGRAAVGGSAAA----ASAAAGNDAVDHFLKSRGLYG--SQIELSFSATNM 54

Query: 187  QDRDILSKNDPMAVFYAKGRDGKLEELGRTEVVLNSLNPTWITKYVITYQFEVVQHMVFH 366
            +DRD+LSK+DPM V Y KGRDG L E  RTEVVLNSLNPTWI K+ IT+QFEVVQ +VFH
Sbjct: 55   RDRDVLSKSDPMLVGYTKGRDGTLTEAFRTEVVLNSLNPTWIAKHTITFQFEVVQTLVFH 114

Query: 367  VYDVDTQFHNVPVKMLKLDEQQFLGEATCALSEIVTKPSRSLTLDIICREESNRSDRPRR 546
             YDVDTQFHN+ VKMLKL+EQQFLGEA+C LSE+VT+PSRSLTLD++ RE+      PRR
Sbjct: 115  AYDVDTQFHNIDVKMLKLEEQQFLGEASCVLSEVVTRPSRSLTLDLVYREDPTIPGNPRR 174

Query: 547  LGELTVHAEECIGSKTMTEMIFRCSDLEYKDLFSKSDPFLVISRTVESGGTIPVCKTEVK 726
             G+L VHAEECI SKT TEMI +CS+LE KDLFS+SDPFL+IS+ VE+G  IPVCKTEV 
Sbjct: 175  CGQLIVHAEECISSKTTTEMILKCSNLEQKDLFSRSDPFLLISKIVEAGLPIPVCKTEVL 234

Query: 727  KNDLNPTWKPVCLNIQQVGSKDSPLHIECLNFNSNGRHDLIGKVQKSLVDLEKLQHNEQG 906
            KND +P WKPV L+IQQVGSK++PL IEC NFNS+G+HDLIGKVQKSL +LEKL  + +G
Sbjct: 235  KNDNHPIWKPVFLSIQQVGSKENPLMIECFNFNSSGKHDLIGKVQKSLAELEKLHSSGEG 294

Query: 907  ESMFLPTVVGHSYQNKVLKSRIFVDKFSECVQNTFLDYIAGGCELNFMVAVDFTASNGNP 1086
            E +FLPT VGH+YQNKVLKS+IFV+ FS+  ++TFLDY+ GGCELNFMVA+DFTASNGNP
Sbjct: 295  EHLFLPTTVGHNYQNKVLKSQIFVENFSQSSRHTFLDYLTGGCELNFMVAIDFTASNGNP 354

Query: 1087 RLPDSLHYIDPSGRPNAYQKAILEVGGVLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNG 1266
            RLPDSLHY+DPSGR NAYQ+AI+EVG VLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNG
Sbjct: 355  RLPDSLHYLDPSGRLNAYQRAIIEVGEVLQFYDSDKRFPAWGFGARPIDGPVSHCFNLNG 414

Query: 1267 SSYHSEVEGIQGIMAAYASAIPNVSLAGPTLFGPVINNASIIASHALANNLQKYFVLLII 1446
            S+ H EV+G+QGIM AY SA+ NVSLAGPTLFGPVI+NA++IAS +LAN  +KYFVLLII
Sbjct: 415  SNNHCEVDGVQGIMIAYTSALYNVSLAGPTLFGPVISNAALIASQSLANGGRKYFVLLII 474

Query: 1447 TDGVITDLQETKDALVKASDLPLSILIVGVGGADFKEMEVLDADKGDRLESSAGRIASRN 1626
            TDGV+TDLQETKDA+VKASDLPLSILIVGVGGADFKEME+LDADKGDRLESS GR+ASR+
Sbjct: 475  TDGVVTDLQETKDAIVKASDLPLSILIVGVGGADFKEMEILDADKGDRLESSTGRVASRD 534

Query: 1627 IVQFVPFRDVQSGEILVLQSLLAELPVQFLTYMRSRDIRP 1746
            IVQF+P RDVQSGEI V+Q LLAELP QFL+YMRSR+I+P
Sbjct: 535  IVQFIPLRDVQSGEISVVQELLAELPTQFLSYMRSRNIQP 574