BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00016888
         (1460 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285915.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   653   0.0  
ref|XP_003634474.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   643   0.0  
emb|CBI18888.3| unnamed protein product [Vitis vinifera]              642   0.0  
ref|XP_002307125.1| predicted protein [Populus trichocarpa] gi|2...   630   e-178
ref|NP_181956.2| cytoplasmic tRNA 2-thiolation protein 1 [Arabid...   626   e-177

>ref|XP_002285915.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like isoform 1
            [Vitis vinifera]
          Length = 356

 Score =  653 bits (1684), Expect = 0.0
 Identities = 320/356 (89%), Positives = 335/356 (94%), Gaps = 1/356 (0%)
 Frame = -2

Query: 1252 MEDTEGKQKKAGVRQCCICNQRKAALKRPKTLEQICRECFYAVFEEEIHRVIVDNQLFKS 1073
            M + E K KKAG  QC IC+QR+AALKRPKTLEQICRECFYAVFEEEIHRV+VDNQLFK 
Sbjct: 1    MGEAEVKPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKP 60

Query: 1072 GERIAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQ 893
            GERIAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNE+Q
Sbjct: 61   GERIAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQ 120

Query: 892  YGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGASLLKVDKLATGHNA 713
            YGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGA++LKVDKLATGHNA
Sbjct: 121  YGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAAMLKVDKLATGHNA 180

Query: 712  DDIAETILLNILRGDIARLGRCTSIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFS 533
            DDIAET+LLNILRGDIARL RCTSIITGEDGPIPRCKPFKYTYEKEIVMYAYFK+LDYFS
Sbjct: 181  DDIAETVLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKYTYEKEIVMYAYFKRLDYFS 240

Query: 532  TECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRISTSTKMPEQGTCERCGYIS 353
            TECIYSPNAYRGFAREFIKDLERIRPRAILDII+SGE FRISTS KMPEQGTCERCGYIS
Sbjct: 241  TECIYSPNAYRGFAREFIKDLERIRPRAILDIIRSGEKFRISTSAKMPEQGTCERCGYIS 300

Query: 352  SQKWCKACVLLEGLNRGLPRLGIGRTKGL-NGHMSGVEKSDDANNIQSKPCGTLDF 188
            SQKWCKACVLLEGLNRGLP+LGIGRT+GL NG     ++S+   NIQSK CGTLDF
Sbjct: 301  SQKWCKACVLLEGLNRGLPKLGIGRTRGLNNGLECDTKQSNGVKNIQSKQCGTLDF 356


>ref|XP_003634474.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like isoform 2
            [Vitis vinifera]
          Length = 363

 Score =  643 bits (1658), Expect = 0.0
 Identities = 318/363 (87%), Positives = 334/363 (92%), Gaps = 8/363 (2%)
 Frame = -2

Query: 1252 MEDTEGKQKKAGVRQCCICNQRKAALKRPKTLEQICRECFYAVFEEEIHRVIVDNQLFKS 1073
            M + E K KKAG  QC IC+QR+AALKRPKTLEQICRECFYAVFEEEIHRV+VDNQLFK 
Sbjct: 1    MGEAEVKPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKP 60

Query: 1072 GERIAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQ 893
            GERIAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNE+Q
Sbjct: 61   GERIAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQ 120

Query: 892  YGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGASLLKVDKLATGHNA 713
            YGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGA++LKVDKLATGHNA
Sbjct: 121  YGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAAMLKVDKLATGHNA 180

Query: 712  DDIAETILLNILRGDIARLGRCTSIITGEDGPIPRCKPFKYTYEKEIVM-------YAYF 554
            DDIAET+LLNILRGDIARL RCTSIITGEDGPIPRCKPFKYTYEKEI+        YAYF
Sbjct: 181  DDIAETVLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKYTYEKEILQNLNRIYTYAYF 240

Query: 553  KKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRISTSTKMPEQGTC 374
            K+LDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDII+SGE FRISTS KMPEQGTC
Sbjct: 241  KRLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIRSGEKFRISTSAKMPEQGTC 300

Query: 373  ERCGYISSQKWCKACVLLEGLNRGLPRLGIGRTKGL-NGHMSGVEKSDDANNIQSKPCGT 197
            ERCGYISSQKWCKACVLLEGLNRGLP+LGIGRT+GL NG     ++S+   NIQSK CGT
Sbjct: 301  ERCGYISSQKWCKACVLLEGLNRGLPKLGIGRTRGLNNGLECDTKQSNGVKNIQSKQCGT 360

Query: 196  LDF 188
            LDF
Sbjct: 361  LDF 363


>emb|CBI18888.3| unnamed protein product [Vitis vinifera]
          Length = 370

 Score =  642 bits (1655), Expect = 0.0
 Identities = 319/370 (86%), Positives = 335/370 (90%), Gaps = 15/370 (4%)
 Frame = -2

Query: 1252 MEDTEGKQKKAGVRQCCICNQRKAALKRPKTLEQICRECFYAVFEEEIHRVIVDNQLFKS 1073
            M + E K KKAG  QC IC+QR+AALKRPKTLEQICRECFYAVFEEEIHRV+VDNQLFK 
Sbjct: 1    MGEAEVKPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKP 60

Query: 1072 GERIAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQ 893
            GERIAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNE+Q
Sbjct: 61   GERIAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQ 120

Query: 892  YGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGASLLKVDKLATGHNA 713
            YGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGA++LKVDKLATGHNA
Sbjct: 121  YGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAAMLKVDKLATGHNA 180

Query: 712  DDIAETILLNILRGDIARLGRCTSIITGEDGPIPRCKPFKYTYEKEIVM----------- 566
            DDIAET+LLNILRGDIARL RCTSIITGEDGPIPRCKPFKYTYEKEIV+           
Sbjct: 181  DDIAETVLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKYTYEKEIVIYPYSFLQNLNR 240

Query: 565  ---YAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRISTSTK 395
               YAYFK+LDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDII+SGE FRISTS K
Sbjct: 241  IYTYAYFKRLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIRSGEKFRISTSAK 300

Query: 394  MPEQGTCERCGYISSQKWCKACVLLEGLNRGLPRLGIGRTKGL-NGHMSGVEKSDDANNI 218
            MPEQGTCERCGYISSQKWCKACVLLEGLNRGLP+LGIGRT+GL NG     ++S+   NI
Sbjct: 301  MPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKLGIGRTRGLNNGLECDTKQSNGVKNI 360

Query: 217  QSKPCGTLDF 188
            QSK CGTLDF
Sbjct: 361  QSKQCGTLDF 370


>ref|XP_002307125.1| predicted protein [Populus trichocarpa] gi|222856574|gb|EEE94121.1|
            predicted protein [Populus trichocarpa]
          Length = 354

 Score =  630 bits (1626), Expect = e-178
 Identities = 307/349 (87%), Positives = 327/349 (93%), Gaps = 2/349 (0%)
 Frame = -2

Query: 1228 KKAGVRQCCICNQRKAALKRPKTLEQICRECFYAVFEEEIHRVIVDNQLFKSGERIAIGA 1049
            KKA  R C +CNQR+AALKRPKTLEQICRECFY VFEEEIH+VIV NQLFK GERIAIGA
Sbjct: 6    KKAAGRLCYLCNQRRAALKRPKTLEQICRECFYEVFEEEIHQVIVKNQLFKPGERIAIGA 65

Query: 1048 SGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQYGLPLKVV 869
            SGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNE+QYGLPLK+V
Sbjct: 66   SGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLKIV 125

Query: 868  SYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGASLLKVDKLATGHNADDIAETIL 689
            SYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGA+LLKVDKL TGHNADDIAET+L
Sbjct: 126  SYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDIAETVL 185

Query: 688  LNILRGDIARLGRCTSIITGEDGPIPRCKPFKYTYEKEIVM-YAYFKKLDYFSTECIYSP 512
            LNILRGDIARL RCTSI TGEDGPIPRCKPFKYTYEKEI++ YAYFK+LDYFSTECIYSP
Sbjct: 186  LNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIMLTYAYFKRLDYFSTECIYSP 245

Query: 511  NAYRGFAREFIKDLERIRPRAILDIIKSGESFRISTSTKMPEQGTCERCGYISSQKWCKA 332
            NAYRGFAREFIKDLER+RPRAILDIIKSGE FRISTSTKMPEQGTCERCGYISSQKWCKA
Sbjct: 246  NAYRGFAREFIKDLERMRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYISSQKWCKA 305

Query: 331  CVLLEGLNRGLPRLGIGRTKGL-NGHMSGVEKSDDANNIQSKPCGTLDF 188
            CVLLEGLN+GLP+LGIGR++GL N H   V++S+   NI+ K CG+LDF
Sbjct: 306  CVLLEGLNKGLPKLGIGRSRGLDNNHRKDVKQSNGTKNIEGKQCGSLDF 354


>ref|NP_181956.2| cytoplasmic tRNA 2-thiolation protein 1 [Arabidopsis thaliana]
            gi|226711456|sp|O64862.2|CTU1_ARATH RecName:
            Full=Cytoplasmic tRNA 2-thiolation protein 1; AltName:
            Full=Cytoplasmic tRNA adenylyltransferase 1
            gi|26449617|dbj|BAC41934.1| unknown protein [Arabidopsis
            thaliana] gi|30725380|gb|AAP37712.1| At2g44270
            [Arabidopsis thaliana] gi|330255305|gb|AEC10399.1|
            cytoplasmic tRNA 2-thiolation protein 1 [Arabidopsis
            thaliana]
          Length = 355

 Score =  626 bits (1615), Expect = e-177
 Identities = 305/354 (86%), Positives = 327/354 (92%), Gaps = 3/354 (0%)
 Frame = -2

Query: 1240 EGKQKKA-GVRQCCICNQRKAALKRPKTLEQICRECFYAVFEEEIHRVIVDNQLFKSGER 1064
            E K KKA   R CC+CN R+  LKRPKTL+QICRECFY VFEEEIH+VIV N+LFKSGER
Sbjct: 2    EAKNKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGER 61

Query: 1063 IAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNELQYGL 884
            +AIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLS+DEGITGYRDDSLETVKRNE+QYGL
Sbjct: 62   VAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSIDEGITGYRDDSLETVKRNEVQYGL 121

Query: 883  PLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGASLLKVDKLATGHNADDI 704
            PLK+VSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGA+LLKV+KL TGHNADDI
Sbjct: 122  PLKIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDI 181

Query: 703  AETILLNILRGDIARLGRCTSIITGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC 524
            AET+LLNILRGDIARL RCTSI TGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC
Sbjct: 182  AETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC 241

Query: 523  IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRISTSTKMPEQGTCERCGYISSQK 344
            IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGE FRI+T+TKMPEQGTCERCGYISSQK
Sbjct: 242  IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIATTTKMPEQGTCERCGYISSQK 301

Query: 343  WCKACVLLEGLNRGLPRLGIGRTKGLNG-HMSGVEKSDD-ANNIQSKPCGTLDF 188
            WCKACVLLEGLNRGLP++GIGR +G+NG H    +K    A +I+SK CG+LDF
Sbjct: 302  WCKACVLLEGLNRGLPKMGIGRPRGVNGDHNKETKKPGSVAKSIESKQCGSLDF 355