BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00016681
         (1112 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P81898.2|PNAA_PRUDU RecName: Full=Peptide-N4-(N-acetyl-beta-g...   557   e-156
ref|XP_002285454.1| PREDICTED: peptide-N4-(N-acetyl-beta-glucosa...   537   e-150
ref|XP_002316856.1| predicted protein [Populus trichocarpa] gi|2...   535   e-150
ref|XP_003589332.1| Peptide-N4-(N-acetyl-beta-glucosaminyl)aspar...   512   e-143
ref|XP_002524519.1| Peptide-N4-(N-acetyl-beta-glucosaminyl)aspar...   512   e-143

>sp|P81898.2|PNAA_PRUDU RecName: Full=Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
            amidase A; Short=PNGase A; AltName: Full=Glycopeptide
            N-glycosidase; AltName: Full=N-glycanase; Contains:
            RecName:
            Full=Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
            amidase A light chain; AltName: Full=PNGase A small
            chain; AltName: Full=PNGase A subunit B; Contains:
            RecName:
            Full=Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
            amidase A heavy chain; AltName: Full=PNGase A large
            chain; Short=PNGase A subunit A
          Length = 571

 Score =  557 bits (1435), Expect = e-156
 Identities = 266/370 (71%), Positives = 312/370 (84%)
 Frame = -1

Query: 1112 FSKIVLEWKATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIVWTVEKDITRYASVLKS 933
            FS IVLEWKATC+ RQFDRIFGVWLGGVE+LRSCTAEPR  GIVWTVEKDITRY S+LKS
Sbjct: 61   FSTIVLEWKATCRRRQFDRIFGVWLGGVEILRSCTAEPRPNGIVWTVEKDITRYYSLLKS 120

Query: 932  PQTLAVYLGNLIDSTYTGIYHVNVTIHFYPAEQPQHGFKQNPADWDSGYGLPADLVLPIS 753
             QTLAVYLGNLID TYTGIYHVN+++HFYPA++  + F+Q   +  SGY   ADL+LPIS
Sbjct: 121  NQTLAVYLGNLIDKTYTGIYHVNISLHFYPAKEKLNSFQQKLDNLASGYHSWADLILPIS 180

Query: 752  RKLPLNDGQWFFVQNSTDVQLKEFSIPRNAYRAVLEVYVSPHQNDEFWYGNPPNDYLIAN 573
            R LPLNDG WF VQNS D +LKEF IP+NAYRAVLEVYVS H+NDEFWY N PN+Y+ AN
Sbjct: 181  RNLPLNDGLWFEVQNSNDTELKEFKIPQNAYRAVLEVYVSFHENDEFWYSNLPNEYIAAN 240

Query: 572  NLTGTPGNGPFREVVVSLDDKVVGAVFPFTVIYTGGINPLLWRPITGIGSYDLPSYDIDI 393
            NL+GTPGNGPFREVVVSLD +VVGAV+PFTVI+TGGINPLLWRPIT IGS+DLP+YDI+I
Sbjct: 241  NLSGTPGNGPFREVVVSLDGEVVGAVWPFTVIFTGGINPLLWRPITAIGSFDLPTYDIEI 300

Query: 392  TPFLGSILDGKRHTVGFGVTNALDLWYIDANLHVWLDPKSTETKGELIEYKSVPSKVSLV 213
            TPFLG ILDGK H  GF VTNAL++WY+DANLH+WLD +ST+T+G+L ++ S+P  VSLV
Sbjct: 301  TPFLGKILDGKSHKFGFNVTNALNVWYVDANLHLWLDKQSTKTEGKLSKHSSLPLVVSLV 360

Query: 212  SNFSGLNGTFLTKACRSVYSSGWVESSHGKIVTHSFQDFSFVNLMRMGKNANLQVVNQSI 33
            S+F GLNGTFLT+  RSV S+GWV+SS+G I T S QDF + N M +GK+ N+Q+VNQ I
Sbjct: 361  SDFKGLNGTFLTRTSRSVSSTGWVKSSYGNITTRSIQDFYYSNSMVLGKDGNMQIVNQKI 420

Query: 32   GSNHTVYSNL 3
              N +VY NL
Sbjct: 421  IFNDSVYINL 430


>ref|XP_002285454.1| PREDICTED: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
            A-like [Vitis vinifera]
          Length = 612

 Score =  537 bits (1383), Expect = e-150
 Identities = 255/368 (69%), Positives = 301/368 (81%)
 Frame = -1

Query: 1112 FSKIVLEWKATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIVWTVEKDITRYASVLKS 933
            FSKIVLEW ATCKGRQFDRIFGVWLGGVELLRSCTAEP ATGIVWTV+KD+TRY S+L  
Sbjct: 100  FSKIVLEWSATCKGRQFDRIFGVWLGGVELLRSCTAEPTATGIVWTVKKDVTRYYSLLMK 159

Query: 932  PQTLAVYLGNLIDSTYTGIYHVNVTIHFYPAEQPQHGFKQNPADWDSGYGLPADLVLPIS 753
             +TLAVYLGN++D TYTG+YHVNVT HFYPA +       N     SGYG  ADL+LPIS
Sbjct: 160  EETLAVYLGNVVDKTYTGVYHVNVTFHFYPAAK-------NSEKLASGYGSWADLILPIS 212

Query: 752  RKLPLNDGQWFFVQNSTDVQLKEFSIPRNAYRAVLEVYVSPHQNDEFWYGNPPNDYLIAN 573
            R LPLNDG WF ++NSTD+++K+F IPRNAYRAVLEVY+S H+NDEFWY NPPNDY+ AN
Sbjct: 213  RNLPLNDGLWFEIENSTDLEVKKFKIPRNAYRAVLEVYLSFHENDEFWYSNPPNDYISAN 272

Query: 572  NLTGTPGNGPFREVVVSLDDKVVGAVFPFTVIYTGGINPLLWRPITGIGSYDLPSYDIDI 393
            NLTGTPGNGPFREV+V LD K+VGAV+PFTVIYTGG+NPLLWRPITGIGS+DLPSY+I+I
Sbjct: 273  NLTGTPGNGPFREVLVGLDGKLVGAVWPFTVIYTGGVNPLLWRPITGIGSFDLPSYNIEI 332

Query: 392  TPFLGSILDGKRHTVGFGVTNALDLWYIDANLHVWLDPKSTETKGELIEYKSVPSKVSLV 213
            TPFLG ILDGK HT  F VTNAL++WYIDANLH+WLD KST+T+G L+ + S  S  +  
Sbjct: 333  TPFLGKILDGKTHTFEFSVTNALNVWYIDANLHIWLDHKSTQTEGRLLGHGSGSSLSTST 392

Query: 212  SNFSGLNGTFLTKACRSVYSSGWVESSHGKIVTHSFQDFSFVNLMRMGKNANLQVVNQSI 33
                GLN +FLT + RS+ S+GWV+SSHGK+ T S Q+F ++NLM MG + +LQ VNQ+I
Sbjct: 393  LYMKGLNASFLTLSSRSISSTGWVKSSHGKMTTQSIQEFKYINLMVMGNDGDLQTVNQTI 452

Query: 32   GSNHTVYS 9
              N  VY+
Sbjct: 453  HFNDGVYT 460


>ref|XP_002316856.1| predicted protein [Populus trichocarpa] gi|222859921|gb|EEE97468.1|
            predicted protein [Populus trichocarpa]
          Length = 631

 Score =  535 bits (1379), Expect = e-150
 Identities = 260/366 (71%), Positives = 305/366 (83%), Gaps = 6/366 (1%)
 Frame = -1

Query: 1112 FSKIVLEWKATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIVWTVEKDITRYASVLKS 933
            FSKIVLEWKATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIVWTV KDITRY S+L  
Sbjct: 109  FSKIVLEWKATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIVWTVRKDITRYYSLLVK 168

Query: 932  PQT--LAVYLGNLIDSTYTGIYHVNVTIHFYPAEQP----QHGFKQNPADWDSGYGLPAD 771
             +T   AVY+GN++DSTYTGIYHVNV+I+FYPAE+      HGF    +  DS     AD
Sbjct: 169  NETQEFAVYMGNIVDSTYTGIYHVNVSIYFYPAEKKLSHSDHGFNNLASGRDS----KAD 224

Query: 770  LVLPISRKLPLNDGQWFFVQNSTDVQLKEFSIPRNAYRAVLEVYVSPHQNDEFWYGNPPN 591
            L+LPISR  PLNDG WF +QNSTD + KEF IP+N YRAVLEVYVS H+NDEFWYGN PN
Sbjct: 225  LILPISRNFPLNDGFWFEIQNSTDSEAKEFKIPQNVYRAVLEVYVSFHENDEFWYGNYPN 284

Query: 590  DYLIANNLTGTPGNGPFREVVVSLDDKVVGAVFPFTVIYTGGINPLLWRPITGIGSYDLP 411
            +Y+IANNLTG PGNGPFREVVVSLD ++VGAV+PFTV++TGGINPLLWRPIT IGS+DLP
Sbjct: 285  EYIIANNLTGFPGNGPFREVVVSLDGEIVGAVWPFTVVFTGGINPLLWRPITAIGSFDLP 344

Query: 410  SYDIDITPFLGSILDGKRHTVGFGVTNALDLWYIDANLHVWLDPKSTETKGELIEYKSVP 231
            SYDI+ITPFLG+ILDGK H +GF VTNAL++WYIDANLH+WLD +ST T+G+L++++S P
Sbjct: 345  SYDIEITPFLGNILDGKTHKLGFSVTNALNVWYIDANLHLWLDHRSTITEGKLLKHESKP 404

Query: 230  SKVSLVSNFSGLNGTFLTKACRSVYSSGWVESSHGKIVTHSFQDFSFVNLMRMGKNANLQ 51
              +SLVSNF+GLNG FLT A R + S+GWV+SSHG I T   Q F + NLM MGK+ +LQ
Sbjct: 405  LALSLVSNFTGLNGKFLTSARRFISSNGWVKSSHGNITTRFNQHFGYRNLMEMGKDGDLQ 464

Query: 50   VVNQSI 33
            +VNQ+I
Sbjct: 465  IVNQTI 470


>ref|XP_003589332.1| Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Medicago
            truncatula] gi|355478380|gb|AES59583.1|
            Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
            A [Medicago truncatula]
          Length = 1183

 Score =  512 bits (1319), Expect = e-143
 Identities = 253/376 (67%), Positives = 300/376 (79%), Gaps = 10/376 (2%)
 Frame = -1

Query: 1112 FSKIVLEWKATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIVWTVEKDITRYASVLKS 933
            FSKIVLEWKATC+GRQFDRIFGVWL GVELLRSCTAEPR  GIVW+VEKDITRY S+L +
Sbjct: 94   FSKIVLEWKATCEGRQFDRIFGVWLDGVELLRSCTAEPRQNGIVWSVEKDITRYQSLLLN 153

Query: 932  PQ---TLAVYLGNLIDSTYTGIYHVNVTIHFYP---AEQPQHGFKQNPAD---WDSGYGL 780
            PQ   TLAV L N++D TYTG+YHV++ IHFY    A   Q+  K    D   + S +  
Sbjct: 154  PQKNQTLAVLLRNIVDKTYTGVYHVDIAIHFYGYHWASFRQNTHKTKKLDCFSFRSDFKS 213

Query: 779  PADLVLPISRKLPLNDGQWFFVQNSTDVQLKEFSIPRNAYRAVLEVYVSPHQNDEFWYGN 600
             ADL+LPISR LP NDG WF +QNSTDV LKEF +P+NAYRAVLEVYVS H+NDEFWY N
Sbjct: 214  HADLILPISRNLPFNDGFWFEIQNSTDVGLKEFVVPQNAYRAVLEVYVSFHENDEFWYTN 273

Query: 599  PPNDYLIANNLT-GTPGNGPFREVVVSLDDKVVGAVFPFTVIYTGGINPLLWRPITGIGS 423
            PPN+YL+ANN+T  TPGNGPFREVVV+LDDKVVG+V+PFTVIYTGG+NPLLWRPITGIGS
Sbjct: 274  PPNEYLVANNITDSTPGNGPFREVVVTLDDKVVGSVWPFTVIYTGGVNPLLWRPITGIGS 333

Query: 422  YDLPSYDIDITPFLGSILDGKRHTVGFGVTNALDLWYIDANLHVWLDPKSTETKGELIEY 243
            +DLPSYDI+ITPFLG+ILDGK+H  GF VTNAL++WYIDANLH+WLD KS+ T+G+L+ +
Sbjct: 334  FDLPSYDIEITPFLGTILDGKKHLFGFSVTNALNVWYIDANLHLWLDTKSSRTEGKLVNH 393

Query: 242  KSVPSKVSLVSNFSGLNGTFLTKACRSVYSSGWVESSHGKIVTHSFQDFSFVNLMRMGKN 63
               P   SLV++F+GLNGTFLT A +S+ ++GWV SS G I T   QDF + + M +GKN
Sbjct: 394  VDKPLVESLVADFNGLNGTFLTSAKKSILATGWVRSSFGNITTSFVQDFDYNSSMVIGKN 453

Query: 62   ANLQVVNQSIGSNHTV 15
               Q VNQ I  N+TV
Sbjct: 454  GGKQAVNQVISFNNTV 469



 Score =  497 bits (1279), Expect = e-138
 Identities = 245/376 (65%), Positives = 295/376 (78%), Gaps = 6/376 (1%)
 Frame = -1

Query: 1112 FSKIVLEWKATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIVWTVEKDITRYASVL-- 939
            FSKIVLEWKATC+GRQ+DRIFGVWLGGVELLRSCTAEP A GIVW+VEKDITRY S+L  
Sbjct: 674  FSKIVLEWKATCEGRQYDRIFGVWLGGVELLRSCTAEPIANGIVWSVEKDITRYKSLLLN 733

Query: 938  -KSPQTLAVYLGNLIDSTYTGIYHVNVTIHF--YPAEQPQHGFKQNPADWDSGYGLPADL 768
             +  QTLAV L N++D TYTG+YHV++TIHF  YP +  +    +         G  ADL
Sbjct: 734  HQQNQTLAVLLRNIVDKTYTGVYHVDITIHFHGYPFDVKKTHKTKKFDSLAFRSGFHADL 793

Query: 767  VLPISRKLPLNDGQWFFVQNSTDVQLKEFSIPRNAYRAVLEVYVSPHQNDEFWYGNPPND 588
            +LPISR LP ND  WF +QNSTDV +KEF +P+NAYRAVLEVYVS H+NDE WY NPPN+
Sbjct: 794  ILPISRNLPKNDVLWFEIQNSTDVGVKEFVVPQNAYRAVLEVYVSFHENDESWYSNPPNE 853

Query: 587  YLIANNLTGT-PGNGPFREVVVSLDDKVVGAVFPFTVIYTGGINPLLWRPITGIGSYDLP 411
            YL+ANN+T + PGNGPFREVVV+LDDKVVG+V+PFTVI+TGG+NPLLWRPIT IGS+DLP
Sbjct: 854  YLVANNITDSNPGNGPFREVVVTLDDKVVGSVWPFTVIFTGGVNPLLWRPITAIGSFDLP 913

Query: 410  SYDIDITPFLGSILDGKRHTVGFGVTNALDLWYIDANLHVWLDPKSTETKGELIEYKSVP 231
            SYDI+ITP LG+ILDGK+H  GF VTNAL++WYIDANLH+WLD KS+ T+GEL+ +   P
Sbjct: 914  SYDIEITPILGTILDGKKHLFGFSVTNALNVWYIDANLHLWLDTKSSRTEGELVNHVDKP 973

Query: 230  SKVSLVSNFSGLNGTFLTKACRSVYSSGWVESSHGKIVTHSFQDFSFVNLMRMGKNANLQ 51
               SLV++F+GLNGTFLT A +S+ S+GWV SS G I T   QDF + N M M KN + Q
Sbjct: 974  LVESLVTDFNGLNGTFLTSAKKSILSTGWVRSSFGNITTSFVQDFDYYNSMVMRKNGSKQ 1033

Query: 50   VVNQSIGSNHTVYSNL 3
             VNQ I  N ++ + L
Sbjct: 1034 TVNQIISFNDSIRAKL 1049


>ref|XP_002524519.1| Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A, putative
            [Ricinus communis] gi|223536193|gb|EEF37846.1|
            Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
            A, putative [Ricinus communis]
          Length = 624

 Score =  512 bits (1318), Expect = e-143
 Identities = 247/369 (66%), Positives = 297/369 (80%), Gaps = 3/369 (0%)
 Frame = -1

Query: 1112 FSKIVLEWKATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIVWTVEKDITRYASVLKS 933
            FSKI+LEW ATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGI W+V+KDITRY S L  
Sbjct: 103  FSKIILEWNATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIFWSVQKDITRYYSSLLK 162

Query: 932  PQT--LAVYLGNLIDSTYTGIYHVNVTIHFYPAEQPQHGFKQNPAD-WDSGYGLPADLVL 762
             +T  LAVYLGNL+DSTYTG+Y VNVT++FYPAE      + +  D + + +   ADL+L
Sbjct: 163  DETQELAVYLGNLVDSTYTGVYRVNVTLYFYPAEDKSSYNENSLLDHFKARHDSKADLIL 222

Query: 761  PISRKLPLNDGQWFFVQNSTDVQLKEFSIPRNAYRAVLEVYVSPHQNDEFWYGNPPNDYL 582
            PISR LPLNDG WF +QNSTD QLKEF IP N YRAVLEVYVS H+NDEFWY N  N+Y+
Sbjct: 223  PISRDLPLNDGLWFQIQNSTDTQLKEFEIPPNVYRAVLEVYVSFHENDEFWYSNYYNEYI 282

Query: 581  IANNLTGTPGNGPFREVVVSLDDKVVGAVFPFTVIYTGGINPLLWRPITGIGSYDLPSYD 402
             ANNLTG+PGNGPFREV+VSLD +V+GA++PFTVIYTGGINPLLWRPIT IGS+DLPSYD
Sbjct: 283  SANNLTGSPGNGPFREVIVSLDGEVLGAIWPFTVIYTGGINPLLWRPITAIGSFDLPSYD 342

Query: 401  IDITPFLGSILDGKRHTVGFGVTNALDLWYIDANLHVWLDPKSTETKGELIEYKSVPSKV 222
            +++TP LGS+LDGK H +GF VTNAL++WYIDANLH+WLD KS + +G++++++  P   
Sbjct: 343  LEMTPLLGSVLDGKTHKLGFSVTNALNVWYIDANLHLWLDHKSKKIEGKVLKHEGKPLAF 402

Query: 221  SLVSNFSGLNGTFLTKACRSVYSSGWVESSHGKIVTHSFQDFSFVNLMRMGKNANLQVVN 42
            SL+SNF  LNGTFL  A RS+ S+GWV+ S GKI TH  Q FS+ N M MG + NLQ+VN
Sbjct: 403  SLISNFKDLNGTFLAAAQRSISSTGWVKCSFGKITTHFNQRFSYNNSMEMGDDGNLQIVN 462

Query: 41   QSIGSNHTV 15
            Q+I    +V
Sbjct: 463  QTINFTDSV 471