BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00013581
         (2135 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]        858   0.0  
ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus ...   837   0.0  
gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]            820   0.0  
gb|AEX32786.1| Coumaroyl-CoA ligase [Vitis vinifera]                  818   0.0  
ref|XP_002325815.1| 4-Coumarate:CoA ligase [Populus trichocarpa]...   816   0.0  

>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
          Length = 565

 Score =  858 bits (2217), Expect = 0.0
 Identities = 431/564 (76%), Positives = 485/564 (85%), Gaps = 4/564 (0%)
 Frame = -3

Query: 1827 MISIASSETT----SPQITPQDSNSDTFVFRSKLPDITIPSHLPLHTYCFERLSEFSERT 1660
            M+S+AS ET     SP  TP++   + F+FRSKLPDI I +HLPLHTYCFE+++EFS+  
Sbjct: 1    MLSVASPETQPQIPSPTPTPENPPVENFIFRSKLPDIPISNHLPLHTYCFEKITEFSDAP 60

Query: 1659 CLISGSNGKVYTFAETHLLCQRSAVGLANLGIKKGDVVMILLQNCPEFVFSFMGASMLGA 1480
            CLISGS+G++YTFAETHL+CQ+SA GL+ LGIKKGDV+MILLQN PEF FSFMGASM+GA
Sbjct: 61   CLISGSSGRIYTFAETHLICQKSAAGLSKLGIKKGDVIMILLQNSPEFAFSFMGASMIGA 120

Query: 1479 ITTTANPFYTPPEIFKQLSSSGAKLIITQSQYVNKIHNSSDNSPKIGKDLTVVTIDDVGQ 1300
            ITTTANPF T  EIFKQ S SGAKL+ITQSQYV+K+ ++ +  PKIG+D TV+T+DD   
Sbjct: 121  ITTTANPFCTSAEIFKQFSVSGAKLVITQSQYVDKLRDTGEKFPKIGEDFTVITVDDP-P 179

Query: 1299 ENCIHFSEICNGDETKVPPVLIELDDAVALPFSSGTTGLPKGVILTHKSLISSIAQQVDG 1120
            E+C+HFS I   DE ++P V I+ DD V++PFSSGTTGLPKGV LTHKSLISS+AQQVDG
Sbjct: 180  ESCLHFSVISGADEREIPSVSIDPDDPVSMPFSSGTTGLPKGVFLTHKSLISSVAQQVDG 239

Query: 1119 ENPNLYLKADDVVLCILPLFHIFSLHSVLLCSLRAGAGVLLMQKFEIGTLLELIQTYRVS 940
            ENPNLYLK DD+VLC+LPLFHI+SL+SVLLCSLRAGAGVLLM KFEI +LLELIQ +RVS
Sbjct: 240  ENPNLYLKTDDIVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMHKFEIRSLLELIQRHRVS 299

Query: 939  XXXXXXXXXXXXAKNPDVEKFDLSSIRIVLSGAAPLGKELEEALKTRLPQAIFGQGYGMT 760
                        AKNP V  FDLSSIRIVLSGAAPLGKELEEAL +RLPQAIFGQGYGMT
Sbjct: 300  VAPVVPPLVLALAKNPMVASFDLSSIRIVLSGAAPLGKELEEALHSRLPQAIFGQGYGMT 359

Query: 759  EVGPVLSMCSGFAKHPFPTKSGSCGTVVRNAELKIIDPETNLSLRHNQAGEICIRGPQIM 580
            E GPVLSMC GFA+  FPTKSGSCGTVVRNAELK+IDPET  SL++NQ GEICIRGPQIM
Sbjct: 360  EAGPVLSMCLGFARQAFPTKSGSCGTVVRNAELKVIDPETGFSLQYNQPGEICIRGPQIM 419

Query: 579  KGYLNDPEATASTIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALL 400
            KGYLND EATASTIDV+GWLHTGD+GYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALL
Sbjct: 420  KGYLNDAEATASTIDVDGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALL 479

Query: 399  VSHPSIADAAVVPQKDTAAGEVPIAFVVRSSDFELTEETVKEFIAKQVVFYKRLHKVYFI 220
            +SHPSIADAAVVPQKD AAGEVP+AFVVRSS FELTEE VKEFIAKQVVFYKRLHKVYF+
Sbjct: 480  ISHPSIADAAVVPQKDEAAGEVPVAFVVRSSGFELTEEAVKEFIAKQVVFYKRLHKVYFV 539

Query: 219  HAIPRSPAGKILRKDLRAKLVMPS 148
            HAIP+S +GKIL KDLRAKL   S
Sbjct: 540  HAIPKSASGKILGKDLRAKLATGS 563


>ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223540792|gb|EEF42352.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 573

 Score =  837 bits (2162), Expect = 0.0
 Identities = 423/571 (74%), Positives = 485/571 (84%), Gaps = 11/571 (1%)
 Frame = -3

Query: 1827 MISIAS---------SETTSPQITPQDSNSDTFVFRSKLPDITIPSHLPLHTYCFERLSE 1675
            MISIAS         S ++S +  P  S+S+T +FRSKLPDI I +HLPLHTYCFE L +
Sbjct: 1    MISIASLEPPKQELSSSSSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLFK 60

Query: 1674 FSERTCLISGSNGKVYTFAETHLLCQRSAVGLANLGIKKGDVVMILLQNCPEFVFSFMGA 1495
             S++ CLISGS GK Y+FAETHL+ Q++A GL++LGIKKGDV+MILLQNCPEFVFSFMGA
Sbjct: 61   LSDKPCLISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMGA 120

Query: 1494 SMLGAITTTANPFYTPPEIFKQLSSSGAKLIITQSQYVNKIHNSSDN-SPKIGKDLTVVT 1318
            SM+GA+ TTANPFYT  EIFKQL+SS  KLIITQS YV+K+ +      PK+G+D TV+T
Sbjct: 121  SMIGAVATTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVIT 180

Query: 1317 IDDVGQENCIHFSEICNGDETKVPPVL-IELDDAVALPFSSGTTGLPKGVILTHKSLISS 1141
            ID+   ENC+HF+ +    ET++P V+    DD VALPFSSGTTGLPKGVILTHKSLI+S
Sbjct: 181  IDEP-PENCLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITS 239

Query: 1140 IAQQVDGENPNLYLKADDVVLCILPLFHIFSLHSVLLCSLRAGAGVLLMQKFEIGTLLEL 961
            +AQQVDGENPNLYLK DDVVLC+LPLFHI+SL+SVLLCSLRAGA VLLMQKF+IG LLEL
Sbjct: 240  VAQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFQIGALLEL 299

Query: 960  IQTYRVSXXXXXXXXXXXXAKNPDVEKFDLSSIRIVLSGAAPLGKELEEALKTRLPQAIF 781
            IQ ++VS            AKNP V +FDLSSIR+VLSGAAPLGKELE+AL++R+PQAI 
Sbjct: 300  IQKHKVSVAAVVPPLVLALAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAIL 359

Query: 780  GQGYGMTEVGPVLSMCSGFAKHPFPTKSGSCGTVVRNAELKIIDPETNLSLRHNQAGEIC 601
            GQGYGMTE GPVLSMC GFAK PFPTKSGSCGTVVRNAELK+IDPET  SL +NQ GEIC
Sbjct: 360  GQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEIC 419

Query: 600  IRGPQIMKGYLNDPEATASTIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPP 421
            IRGPQIMKGYLNDPEATA+TIDVEGWLHTGDIGYVDDDDE+FIVDRVKE+IKFKGFQVPP
Sbjct: 420  IRGPQIMKGYLNDPEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPP 479

Query: 420  AELEALLVSHPSIADAAVVPQKDTAAGEVPIAFVVRSSDFELTEETVKEFIAKQVVFYKR 241
            AELEALL++HPSIADAAVVPQKD  AGEVP+AFVVRS+ FEL+EE VKE+IAKQVVFYK+
Sbjct: 480  AELEALLLNHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELSEEDVKEYIAKQVVFYKK 539

Query: 240  LHKVYFIHAIPRSPAGKILRKDLRAKLVMPS 148
            LHKVYFIHAIP+SP+GKILRKDL+AKL  PS
Sbjct: 540  LHKVYFIHAIPKSPSGKILRKDLKAKLATPS 570


>gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]
          Length = 605

 Score =  820 bits (2117), Expect = 0.0
 Identities = 411/540 (76%), Positives = 465/540 (86%), Gaps = 8/540 (1%)
 Frame = -3

Query: 1755 VFRSKLPDITIPSHLPLHTYCFERLSEFSERTCLISGSNGKVYTFAETHLLCQRSAVGLA 1576
            VFRSKLPDI IP+HLPLHTYCF+ L EFS+R CLI GS GK Y+F+ETHL+ Q++  GL+
Sbjct: 59   VFRSKLPDIAIPNHLPLHTYCFQNLPEFSDRPCLIVGSTGKSYSFSETHLISQKTGAGLS 118

Query: 1575 NLGIKKGDVVMILLQNCPEFVFSFMGASMLGAITTTANPFYTPPEIFKQLSSSGAKLIIT 1396
            NLGI+KGDV+MILLQNC EFVF+FMGAS++GA+TTTANPFYT  EIFKQ+ ++ AKLIIT
Sbjct: 119  NLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTTAEIFKQVKAANAKLIIT 178

Query: 1395 QSQYVNKI--HNSS------DNSPKIGKDLTVVTIDDVGQENCIHFSEICNGDETKVPPV 1240
            QSQYVNK+  H SS      +N PK+G+D  VVTIDD   ENC+HFS +   +E ++P V
Sbjct: 179  QSQYVNKLREHPSSADGADQNNYPKLGEDFKVVTIDDP-PENCLHFSVLSEANEKELPDV 237

Query: 1239 LIELDDAVALPFSSGTTGLPKGVILTHKSLISSIAQQVDGENPNLYLKADDVVLCILPLF 1060
            +I+ +D VALPFSSGTTGLPKGVILTHK+L++S+AQQVDGENPNLYLK DDVVLC+LPLF
Sbjct: 238  VIDAEDPVALPFSSGTTGLPKGVILTHKNLVTSVAQQVDGENPNLYLKEDDVVLCVLPLF 297

Query: 1059 HIFSLHSVLLCSLRAGAGVLLMQKFEIGTLLELIQTYRVSXXXXXXXXXXXXAKNPDVEK 880
            HIFS +SVLLCSLRAGAGVLLM KFEIGTLLELIQ YRVS            AKNP V +
Sbjct: 298  HIFSFNSVLLCSLRAGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMVAE 357

Query: 879  FDLSSIRIVLSGAAPLGKELEEALKTRLPQAIFGQGYGMTEVGPVLSMCSGFAKHPFPTK 700
            FDLSSIR+VLSGAAPLGKELEEALK+R+PQA+ GQGYGMTE GPVLSMC  FAK P PTK
Sbjct: 358  FDLSSIRVVLSGAAPLGKELEEALKSRVPQAVLGQGYGMTEAGPVLSMCMAFAKEPMPTK 417

Query: 699  SGSCGTVVRNAELKIIDPETNLSLRHNQAGEICIRGPQIMKGYLNDPEATASTIDVEGWL 520
            SGSCGTVVRNAELK++DPET LSL +NQ GEICIRG QIMKGYLND  ATA+TID EGWL
Sbjct: 418  SGSCGTVVRNAELKVLDPETGLSLGYNQPGEICIRGFQIMKGYLNDAAATATTIDTEGWL 477

Query: 519  HTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDTAAG 340
            HTGD+GYVDDDDEVFIVDRVKELIKFKGFQVPPAELE+LL+SHPSIADAAVVPQ+D AAG
Sbjct: 478  HTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELESLLISHPSIADAAVVPQRDDAAG 537

Query: 339  EVPIAFVVRSSDFELTEETVKEFIAKQVVFYKRLHKVYFIHAIPRSPAGKILRKDLRAKL 160
            EVP+AFVVRS   ELTEE VKEFIAKQVVFYKRLHKV+F+HAIP+S +GKILRKDLRAKL
Sbjct: 538  EVPVAFVVRSDGLELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDLRAKL 597


>gb|AEX32786.1| Coumaroyl-CoA ligase [Vitis vinifera]
          Length = 570

 Score =  818 bits (2114), Expect = 0.0
 Identities = 411/552 (74%), Positives = 467/552 (84%), Gaps = 1/552 (0%)
 Frame = -3

Query: 1812 SSETTSPQITPQDSNSDTFVFRSKLPDITIPSHLPLHTYCFERLSEFSERTCLISGSNGK 1633
            S+  TS  I    +  D  VFRSKLP+I I +HLPLHTYCFE  S+F++R CLI GS  K
Sbjct: 12   SNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFADRPCLIVGSTNK 71

Query: 1632 VYTFAETHLLCQRSAVGLANLGIKKGDVVMILLQNCPEFVFSFMGASMLGAITTTANPFY 1453
             Y+FAETHL+ ++   G A+LG+K+GDVVMILLQNC EF FSF+GASM+GA+TTTANPFY
Sbjct: 72   TYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVTTTANPFY 131

Query: 1452 TPPEIFKQLSSSGAKLIITQSQYVNKIHNSSDNS-PKIGKDLTVVTIDDVGQENCIHFSE 1276
            T  EIFKQL++S AK+++TQ+QYV+K+ +  D    KIG+  TV+TIDD   ENC+HFS 
Sbjct: 132  TSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDDP-PENCMHFSV 190

Query: 1275 ICNGDETKVPPVLIELDDAVALPFSSGTTGLPKGVILTHKSLISSIAQQVDGENPNLYLK 1096
            +   +E+++P V I  DD VALPFSSGTTGLPKGV+LTHKSLI+S+AQQVDGENPNL+L 
Sbjct: 191  VSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLT 250

Query: 1095 ADDVVLCILPLFHIFSLHSVLLCSLRAGAGVLLMQKFEIGTLLELIQTYRVSXXXXXXXX 916
             DDVVLC+LPLFHI+SL+SVLLCSLRAGA VLLMQKFEIGTLLELIQ YRVS        
Sbjct: 251  PDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRYRVSVAAVVPPL 310

Query: 915  XXXXAKNPDVEKFDLSSIRIVLSGAAPLGKELEEALKTRLPQAIFGQGYGMTEVGPVLSM 736
                AKNP VE FDLSSIR+VLSGAAPLGKELE AL++R+PQA+ GQGYGMTE GPVLSM
Sbjct: 311  VLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGYGMTEAGPVLSM 370

Query: 735  CSGFAKHPFPTKSGSCGTVVRNAELKIIDPETNLSLRHNQAGEICIRGPQIMKGYLNDPE 556
            C GFAK PFPTKSGSCGTVVRNAELK++DPET  SL  NQ GEICIRG QIMKGYLNDPE
Sbjct: 371  CLGFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQQIMKGYLNDPE 430

Query: 555  ATASTIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIAD 376
            ATASTIDV+GWLHTGDIGYVDDD+EVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIAD
Sbjct: 431  ATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIAD 490

Query: 375  AAVVPQKDTAAGEVPIAFVVRSSDFELTEETVKEFIAKQVVFYKRLHKVYFIHAIPRSPA 196
            AAVVPQKD  AGEVP+AFVVRS+ FELTEE VKEFI+KQVVFYKRLHKVYF+HAIP+SP+
Sbjct: 491  AAVVPQKDDVAGEVPVAFVVRSNGFELTEEAVKEFISKQVVFYKRLHKVYFVHAIPKSPS 550

Query: 195  GKILRKDLRAKL 160
            GKILRKDLRAKL
Sbjct: 551  GKILRKDLRAKL 562


>ref|XP_002325815.1| 4-Coumarate:CoA ligase [Populus trichocarpa]
            gi|118487236|gb|ABK95446.1| unknown [Populus trichocarpa]
            gi|222862690|gb|EEF00197.1| 4-Coumarate:CoA ligase
            [Populus trichocarpa]
          Length = 570

 Score =  816 bits (2109), Expect = 0.0
 Identities = 412/564 (73%), Positives = 473/564 (83%), Gaps = 8/564 (1%)
 Frame = -3

Query: 1827 MISIASSETTSPQITPQD------SNSDTFVFRSKLPDITIPSHLPLHTYCFERLSEFSE 1666
            M+S+A+ E   P+++P        S+    +FRSKLPDITI +HLPLH YCFE LS+FS+
Sbjct: 1    MMSVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFSD 60

Query: 1665 RTCLISGSNGKVYTFAETHLLCQRSAVGLANLGIKKGDVVMILLQNCPEFVFSFMGASML 1486
            R CLISGS GK Y+FAETHL+ ++ A GL+NLGIKKGDV+M LLQNCPEFVFSFMGASM+
Sbjct: 61   RPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMI 120

Query: 1485 GAITTTANPFYTPPEIFKQLSSSGAKLIITQSQYVNKIHNSS--DNSPKIGKDLTVVTID 1312
            GA+TTT NPFYTP EIFKQ S+S AKLIITQSQ+VNK+ +S   +N+ K  +D  V+TID
Sbjct: 121  GAVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDCHENNQKPEEDFIVITID 180

Query: 1311 DVGQENCIHFSEICNGDETKVPPVLIELDDAVALPFSSGTTGLPKGVILTHKSLISSIAQ 1132
            D   ENC+HF+ +   +E+++P V I  DD VALPFSSGTTGLPKGVILTHKSLI+S+AQ
Sbjct: 181  DP-PENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 239

Query: 1131 QVDGENPNLYLKADDVVLCILPLFHIFSLHSVLLCSLRAGAGVLLMQKFEIGTLLELIQT 952
            QVDGE PNLYLK DDVVLC+LPLFHIFSL+SVLLCSLRAG+ VLLMQKFEIG+LLELIQ 
Sbjct: 240  QVDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQK 299

Query: 951  YRVSXXXXXXXXXXXXAKNPDVEKFDLSSIRIVLSGAAPLGKELEEALKTRLPQAIFGQG 772
            + VS            AKNP V  FDLSSIR+VLSGAAPLGKELEEAL++R+PQAI GQG
Sbjct: 300  HNVSVAAVVPPLVLALAKNPMVANFDLSSIRVVLSGAAPLGKELEEALRSRVPQAILGQG 359

Query: 771  YGMTEVGPVLSMCSGFAKHPFPTKSGSCGTVVRNAELKIIDPETNLSLRHNQAGEICIRG 592
            YGMTE GPVLSMC  F+K P PTKSGSCGTVVRNAELK+IDPET  SL  NQ GEICIRG
Sbjct: 360  YGMTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGSSLGRNQPGEICIRG 419

Query: 591  PQIMKGYLNDPEATASTIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAEL 412
             QIMKGYLND EATA+ IDVEGWLHTGDIGYVDDDDE+FIVDRVKE+IKFKGFQVPPAEL
Sbjct: 420  SQIMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 479

Query: 411  EALLVSHPSIADAAVVPQKDTAAGEVPIAFVVRSSDFELTEETVKEFIAKQVVFYKRLHK 232
            EALLV+HPSIADAAVVPQKD  AGEVP+AFVVRS+D +L EE VK++IAKQVVFYK+LHK
Sbjct: 480  EALLVNHPSIADAAVVPQKDEVAGEVPVAFVVRSNDLDLNEEAVKDYIAKQVVFYKKLHK 539

Query: 231  VYFIHAIPRSPAGKILRKDLRAKL 160
            V+F+H+IP+S AGKILRKDLRAKL
Sbjct: 540  VFFVHSIPKSAAGKILRKDLRAKL 563