BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00012959
         (2856 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like ...  1169   0.0  
emb|CBI40669.3| unnamed protein product [Vitis vinifera]             1162   0.0  
ref|XP_002324659.1| predicted protein [Populus trichocarpa] gi|2...  1141   0.0  
dbj|BAF52941.1| isoamylase-type starch-debranching enzyme 1 [Pha...  1140   0.0  
gb|AAN15317.1| isoamylase isoform 1 [Solanum tuberosum]              1133   0.0  

>ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like [Vitis vinifera]
          Length = 748

 Score = 1169 bits (3023), Expect = 0.0
 Identities = 539/649 (83%), Positives = 587/649 (90%)
 Frame = -2

Query: 2453 GSTEVAETAVIEKPKLRKFEVFDGSPLPFGATAKDGGVNFAIHSSGAVSATLCLISLSDL 2274
            GS   AET V+EKPKL+ F VF+G P P GATA+DGGVNFA++S  AVSATLCLIS SDL
Sbjct: 12   GSGAEAETVVVEKPKLQPFLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISASDL 71

Query: 2273 QENRVTKKIDLDPLVNKTGDVWHAFLKGDFEGMLYGYTFDGKFSPEEGHCYDSSKILLDP 2094
            +E+RVT++I LDPL NKTGDVWH FLKG+FE ++YGY FDGKFSPEEGH YDSS++LLDP
Sbjct: 72   EEDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSSRLLLDP 131

Query: 2093 YAKAVISREEFGALGAEGNCWPQMGGMVPPADNEFDWEGDLPLMYPQRDLIIYEMHVRGF 1914
            YAKAVISR EFG LG EGNCWP M GM+P +D EFDWEGDLPL YPQ+DLIIYEMHVRGF
Sbjct: 132  YAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYEMHVRGF 191

Query: 1913 TRHDSSGTNFPGTYRGVVEKLDHLKELGVNCIELMPFQEYNEMEYFSYNSVLGNYKLNFW 1734
            TRH+SS T FPGTY GVVEKLDHLKELGVNCIELMP  E+NE+EYFSYNSVL +Y++NFW
Sbjct: 192  TRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYFSYNSVLDDYRVNFW 251

Query: 1733 GYSTINYFSPMLRYSSAGIRNCGQDAINEVKVLVREAHKRGIEVLMDVVFNHTAEGNEKG 1554
            GYST+NYFSPM+RYSSAGI NCG DAINEVK+L+REAHKRGIEVLMDVVFNHTAEGNE G
Sbjct: 252  GYSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHKRGIEVLMDVVFNHTAEGNENG 311

Query: 1553 PILSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFR 1374
            PILSFRGVDNSVYYMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLRYWV EMHVDGFR
Sbjct: 312  PILSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEMHVDGFR 371

Query: 1373 FDLASILTRSSSLWDAVNVYGNSIEGDLLTTGTPLGSPPLIDMISNDPILRRVKLIAEAW 1194
            FDLASI+TR SSLWDA+NVYGN  EGD LTTGTPL SPPLIDMISNDPILR VKLIAEAW
Sbjct: 372  FDLASIMTRGSSLWDALNVYGNPAEGDSLTTGTPLSSPPLIDMISNDPILRGVKLIAEAW 431

Query: 1193 DTGGLYQVGMFPHWGIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKP 1014
            D GGLYQVGMFPHWG+WSEWNGKYRDIVRQFIKG+DGFSGAFAECLCGSPNLYQ GGRKP
Sbjct: 432  DAGGLYQVGMFPHWGLWSEWNGKYRDIVRQFIKGSDGFSGAFAECLCGSPNLYQEGGRKP 491

Query: 1013 WNSINFVCAHDGFTLADLVTYNKKHNLAXXXXXXXXXXXXNSWNCGQEGEFASLSVKRLR 834
            WNSINFVCAHDGFTLADLVTYNKKHN A            NSWNCGQEGEFAS+SVK+LR
Sbjct: 492  WNSINFVCAHDGFTLADLVTYNKKHNNANGEDNNDGENHNNSWNCGQEGEFASISVKKLR 551

Query: 833  KRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESSAEFFR 654
            KRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYC DNY+NYFRWDKKEES ++FFR
Sbjct: 552  KRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYCQDNYMNYFRWDKKEESLSDFFR 611

Query: 653  FCCLMTKFRQECEALGLDDFPTADRLQWHGHAPGVPDWSEISRFVAFSL 507
            FCCLM+KFRQECE+LGL+DFPTA+RLQWHG  PG+PDWS+ SRFVAF++
Sbjct: 612  FCCLMSKFRQECESLGLNDFPTAERLQWHGRTPGMPDWSKTSRFVAFTM 660



 Score =  150 bits (380), Expect = 1e-33
 Identities = 70/90 (77%), Positives = 82/90 (91%), Gaps = 1/90 (1%)
 Frame = -3

Query: 463 SQMDSVKGEIYIAFNTSHLPVTITLPERPGYRWEPLVDTSKPAPYDFLSDDLPEKATATK 284
           + +DSVKGEIY+AFNTSHLP+ ITLPERPGYRW+PLVDTSKPAP+DFLS+D+PE+ TA K
Sbjct: 659 TMVDSVKGEIYVAFNTSHLPIIITLPERPGYRWQPLVDTSKPAPFDFLSNDVPERDTAVK 718

Query: 283 QYSHFLDANMYPMLSYSSIILLLAP-DDNA 197
           QYS F +AN+YPMLSYSSIILLL P D+NA
Sbjct: 719 QYSQFTEANLYPMLSYSSIILLLCPVDENA 748


>emb|CBI40669.3| unnamed protein product [Vitis vinifera]
          Length = 809

 Score = 1162 bits (3006), Expect = 0.0
 Identities = 539/655 (82%), Positives = 587/655 (89%), Gaps = 6/655 (0%)
 Frame = -2

Query: 2453 GSTEVAETAVIEKPKLRKFEVFDGSPLPFGATAKDGGVNFAIHSSGAVSATLCLISLSDL 2274
            GS   AET V+EKPKL+ F VF+G P P GATA+DGGVNFA++S  AVSATLCLIS SDL
Sbjct: 67   GSGAEAETVVVEKPKLQPFLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISASDL 126

Query: 2273 QENRVTKKIDLDPLVNKTGDVWHAFLKGDFEGMLYGYTFDGKFSPEEGHCYDSSKILLDP 2094
            +E+RVT++I LDPL NKTGDVWH FLKG+FE ++YGY FDGKFSPEEGH YDSS++LLDP
Sbjct: 127  EEDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSSRLLLDP 186

Query: 2093 YAKAVISREEFGALGAEGNCWPQMGGMVPPADNEFDWEGDLPLMYPQRDLIIYEMHVRGF 1914
            YAKAVISR EFG LG EGNCWP M GM+P +D EFDWEGDLPL YPQ+DLIIYEMHVRGF
Sbjct: 187  YAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYEMHVRGF 246

Query: 1913 TRHDSSGTNFPGTYRGVVEKLDHLKELGVNCIELMPFQEYNEMEYFSYNSVLGNYK---- 1746
            TRH+SS T FPGTY GVVEKLDHLKELGVNCIELMP  E+NE+EYFSYNSVL +Y+    
Sbjct: 247  TRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYFSYNSVLDDYRYTCI 306

Query: 1745 --LNFWGYSTINYFSPMLRYSSAGIRNCGQDAINEVKVLVREAHKRGIEVLMDVVFNHTA 1572
              +NFWGYST+NYFSPM+RYSSAGI NCG DAINEVK+L+REAHKRGIEVLMDVVFNHTA
Sbjct: 307  SMVNFWGYSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHKRGIEVLMDVVFNHTA 366

Query: 1571 EGNEKGPILSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEM 1392
            EGNE GPILSFRGVDNSVYYMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLRYWV EM
Sbjct: 367  EGNENGPILSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEM 426

Query: 1391 HVDGFRFDLASILTRSSSLWDAVNVYGNSIEGDLLTTGTPLGSPPLIDMISNDPILRRVK 1212
            HVDGFRFDLASI+TR SSLWDA+NVYGN  EGD LTTGTPL SPPLIDMISNDPILR VK
Sbjct: 427  HVDGFRFDLASIMTRGSSLWDALNVYGNPAEGDSLTTGTPLSSPPLIDMISNDPILRGVK 486

Query: 1211 LIAEAWDTGGLYQVGMFPHWGIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQ 1032
            LIAEAWD GGLYQVGMFPHWG+WSEWNGKYRDIVRQFIKG+DGFSGAFAECLCGSPNLYQ
Sbjct: 487  LIAEAWDAGGLYQVGMFPHWGLWSEWNGKYRDIVRQFIKGSDGFSGAFAECLCGSPNLYQ 546

Query: 1031 GGGRKPWNSINFVCAHDGFTLADLVTYNKKHNLAXXXXXXXXXXXXNSWNCGQEGEFASL 852
             GGRKPWNSINFVCAHDGFTLADLVTYNKKHN A            NSWNCGQEGEFAS+
Sbjct: 547  EGGRKPWNSINFVCAHDGFTLADLVTYNKKHNNANGEDNNDGENHNNSWNCGQEGEFASI 606

Query: 851  SVKRLRKRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEES 672
            SVK+LRKRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYC DNY+NYFRWDKKEES
Sbjct: 607  SVKKLRKRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYCQDNYMNYFRWDKKEES 666

Query: 671  SAEFFRFCCLMTKFRQECEALGLDDFPTADRLQWHGHAPGVPDWSEISRFVAFSL 507
             ++FFRFCCLM+KFRQECE+LGL+DFPTA+RLQWHG  PG+PDWS+ SRFVAF++
Sbjct: 667  LSDFFRFCCLMSKFRQECESLGLNDFPTAERLQWHGRTPGMPDWSKTSRFVAFTM 721



 Score =  150 bits (380), Expect = 1e-33
 Identities = 70/90 (77%), Positives = 82/90 (91%), Gaps = 1/90 (1%)
 Frame = -3

Query: 463 SQMDSVKGEIYIAFNTSHLPVTITLPERPGYRWEPLVDTSKPAPYDFLSDDLPEKATATK 284
           + +DSVKGEIY+AFNTSHLP+ ITLPERPGYRW+PLVDTSKPAP+DFLS+D+PE+ TA K
Sbjct: 720 TMVDSVKGEIYVAFNTSHLPIIITLPERPGYRWQPLVDTSKPAPFDFLSNDVPERDTAVK 779

Query: 283 QYSHFLDANMYPMLSYSSIILLLAP-DDNA 197
           QYS F +AN+YPMLSYSSIILLL P D+NA
Sbjct: 780 QYSQFTEANLYPMLSYSSIILLLCPVDENA 809


>ref|XP_002324659.1| predicted protein [Populus trichocarpa] gi|222866093|gb|EEF03224.1|
            predicted protein [Populus trichocarpa]
          Length = 801

 Score = 1141 bits (2952), Expect = 0.0
 Identities = 546/710 (76%), Positives = 599/710 (84%), Gaps = 6/710 (0%)
 Frame = -2

Query: 2618 MELAQIASHS-----FLSPIWRNSQFYPKIQQKRSSQRRSENSLIMKKMKSFAINA-RDG 2457
            M L    SHS     F+S      +F    +   S+     ++L      S +I A RDG
Sbjct: 1    MNLLHSTSHSVHVLKFISQFRSTPKFCLLKRNVTSNLENFGSNLFSNSTASISIKAARDG 60

Query: 2456 GGSTEVAETAVIEKPKLRKFEVFDGSPLPFGATAKDGGVNFAIHSSGAVSATLCLISLSD 2277
              +  V E    E PKLRKF+VF+G P PFGAT +DGGVNFAI S+ AVSATLCLISLSD
Sbjct: 61   VDTAVVVEE---EGPKLRKFQVFEGHPSPFGATVRDGGVNFAIFSANAVSATLCLISLSD 117

Query: 2276 LQENRVTKKIDLDPLVNKTGDVWHAFLKGDFEGMLYGYTFDGKFSPEEGHCYDSSKILLD 2097
            L ENRVT++  LDPL NKTGDVWH  LKGDF+ MLYGY FDG FSPEEGH YD S+I+LD
Sbjct: 118  LPENRVTEQFFLDPLTNKTGDVWHVHLKGDFKDMLYGYKFDGSFSPEEGHYYDPSQIVLD 177

Query: 2096 PYAKAVISREEFGALGAEGNCWPQMGGMVPPADNEFDWEGDLPLMYPQRDLIIYEMHVRG 1917
            PYAKAVISR +FG LG++ NCWPQM  M+P AD++FDWEGD  L YPQRDLI+YEMHVRG
Sbjct: 178  PYAKAVISRGDFGVLGSDDNCWPQMACMIPTADDKFDWEGDSLLKYPQRDLILYEMHVRG 237

Query: 1916 FTRHDSSGTNFPGTYRGVVEKLDHLKELGVNCIELMPFQEYNEMEYFSYNSVLGNYKLNF 1737
            FT+H+SS T FPGTY GVVEKL HLKELGVNCIELMP  E+NE+EY+ YNSV G+YK+NF
Sbjct: 238  FTQHESSRTEFPGTYLGVVEKLGHLKELGVNCIELMPCHEFNELEYYDYNSVFGDYKVNF 297

Query: 1736 WGYSTINYFSPMLRYSSAGIRNCGQDAINEVKVLVREAHKRGIEVLMDVVFNHTAEGNEK 1557
            WGYST+NYFSPM RYSSAG  NCG DAINE K+LVREAHKRGIEV MDVVFNHTAEGNE+
Sbjct: 298  WGYSTVNYFSPMTRYSSAGTLNCGHDAINEFKLLVREAHKRGIEVFMDVVFNHTAEGNER 357

Query: 1556 GPILSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGF 1377
            GPILSF+GVDNSVYYMLAPKGE+YNYSGCGNTFNCNHPVVRQFI+DCLRYWV EMHVDGF
Sbjct: 358  GPILSFKGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVIEMHVDGF 417

Query: 1376 RFDLASILTRSSSLWDAVNVYGNSIEGDLLTTGTPLGSPPLIDMISNDPILRRVKLIAEA 1197
            RFDLASI+TRSSSLWDAVNV+G+ IEGDL+TTGTPLGSPPLI+M+SNDPILR VKLIAEA
Sbjct: 418  RFDLASIMTRSSSLWDAVNVFGSPIEGDLVTTGTPLGSPPLIEMMSNDPILRDVKLIAEA 477

Query: 1196 WDTGGLYQVGMFPHWGIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRK 1017
            WD GGLYQVGMFPHW IWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSP LYQ GGRK
Sbjct: 478  WDAGGLYQVGMFPHWHIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPGLYQEGGRK 537

Query: 1016 PWNSINFVCAHDGFTLADLVTYNKKHNLAXXXXXXXXXXXXNSWNCGQEGEFASLSVKRL 837
            PWNSINFVCAHDGFTLADLVTYN+KHNLA            NSWNCGQEGE AS+SVK+L
Sbjct: 538  PWNSINFVCAHDGFTLADLVTYNEKHNLANGEDNNDGENHNNSWNCGQEGELASISVKKL 597

Query: 836  RKRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESSAEFF 657
            RKRQMRNFFLCLMVSQGVPMI+MGDEYGHTKGGNNNTYCHDNYINYF WDKKEESS++FF
Sbjct: 598  RKRQMRNFFLCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFWWDKKEESSSDFF 657

Query: 656  RFCCLMTKFRQECEALGLDDFPTADRLQWHGHAPGVPDWSEISRFVAFSL 507
            RFC LMTKFR ECE+LGL+DFPTA+RLQWHGHAPG PDWSE SRFVAF+L
Sbjct: 658  RFCRLMTKFRHECESLGLNDFPTAERLQWHGHAPGTPDWSETSRFVAFTL 707



 Score =  149 bits (375), Expect = 5e-33
 Identities = 71/87 (81%), Positives = 77/87 (88%)
 Frame = -3

Query: 457 MDSVKGEIYIAFNTSHLPVTITLPERPGYRWEPLVDTSKPAPYDFLSDDLPEKATATKQY 278
           +DSVK EIYIAFN SHL VTITLPERP YRWEPLVDT KPAP+DFLS DLPE+ TA KQY
Sbjct: 708 IDSVKREIYIAFNASHLSVTITLPERPAYRWEPLVDTGKPAPFDFLSGDLPERDTAMKQY 767

Query: 277 SHFLDANMYPMLSYSSIILLLAPDDNA 197
           SHFL+AN+YPMLSYSSIIL+L P DNA
Sbjct: 768 SHFLEANLYPMLSYSSIILVLLPVDNA 794


>dbj|BAF52941.1| isoamylase-type starch-debranching enzyme 1 [Phaseolus vulgaris]
          Length = 791

 Score = 1140 bits (2948), Expect = 0.0
 Identities = 532/677 (78%), Positives = 589/677 (87%), Gaps = 1/677 (0%)
 Frame = -2

Query: 2534 RSSQRRSENSLIMKKMKSFAINARDGGGSTEVAETAVIEKPKLRK-FEVFDGSPLPFGAT 2358
            R SQ    +S+I    ++ A  AR+GG      ETAV+EKP+L   F+V  G   PFGAT
Sbjct: 32   RFSQLHETDSVIRTVARTRA--ARNGGAVD--TETAVVEKPQLESLFQVSRGYASPFGAT 87

Query: 2357 AKDGGVNFAIHSSGAVSATLCLISLSDLQENRVTKKIDLDPLVNKTGDVWHAFLKGDFEG 2178
             +DGGVNFAI+S  A SATLCL +LSD Q NRVT+ + LDPL+NKTG +WH FLKGDF  
Sbjct: 88   VRDGGVNFAIYSLNAFSATLCLFTLSDFQNNRVTESVPLDPLINKTGGIWHVFLKGDFSD 147

Query: 2177 MLYGYTFDGKFSPEEGHCYDSSKILLDPYAKAVISREEFGALGAEGNCWPQMGGMVPPAD 1998
            MLYGY FDGKFSP EGH YDSS+I+LDPYAKAVISR EFGALG  GNCWPQM GMVP   
Sbjct: 148  MLYGYKFDGKFSPLEGHYYDSSRIVLDPYAKAVISRGEFGALGPNGNCWPQMAGMVPSDH 207

Query: 1997 NEFDWEGDLPLMYPQRDLIIYEMHVRGFTRHDSSGTNFPGTYRGVVEKLDHLKELGVNCI 1818
            +EFDWEGDLPL YPQ+DL++YEMHVRGFT+H+SS T FPGTY GVVEKLDHLKELGVNC+
Sbjct: 208  DEFDWEGDLPLKYPQKDLVVYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLKELGVNCL 267

Query: 1817 ELMPFQEYNEMEYFSYNSVLGNYKLNFWGYSTINYFSPMLRYSSAGIRNCGQDAINEVKV 1638
            ELMP  E+NE+EY SYNSV G+Y++NFWGYSTIN+FSPM+RYSSAGIRNCG+D INE+K 
Sbjct: 268  ELMPCHEFNELEYSSYNSVQGDYRVNFWGYSTINFFSPMIRYSSAGIRNCGRDGINEIKF 327

Query: 1637 LVREAHKRGIEVLMDVVFNHTAEGNEKGPILSFRGVDNSVYYMLAPKGEYYNYSGCGNTF 1458
            L++EAHKRGIEV+MDVVFNHTAEGNE GPI+SFRGVDNS+YYM+APKGE+YNYSGCGNTF
Sbjct: 328  LIKEAHKRGIEVIMDVVFNHTAEGNEDGPIISFRGVDNSIYYMVAPKGEFYNYSGCGNTF 387

Query: 1457 NCNHPVVRQFIVDCLRYWVEEMHVDGFRFDLASILTRSSSLWDAVNVYGNSIEGDLLTTG 1278
            NC+HPVVRQFIVDCLRYWV EMHVDGFRFDLASI+TRSSSLWDA NV+G  IEGDLLTTG
Sbjct: 388  NCSHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDATNVFGAPIEGDLLTTG 447

Query: 1277 TPLGSPPLIDMISNDPILRRVKLIAEAWDTGGLYQVGMFPHWGIWSEWNGKYRDIVRQFI 1098
            TPLGSPPLID+ISNDPILR VKLIAEAWD GGLYQVG FPHWGIWSEWNGKYRD VRQFI
Sbjct: 448  TPLGSPPLIDLISNDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRDTVRQFI 507

Query: 1097 KGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFVCAHDGFTLADLVTYNKKHNLAXXXX 918
            KGTDGF+GAFAECLCGSPNLYQGGGRKPWNSINFVCAHDGFTLADLVTY  KHNL+    
Sbjct: 508  KGTDGFAGAFAECLCGSPNLYQGGGRKPWNSINFVCAHDGFTLADLVTYTNKHNLSNGED 567

Query: 917  XXXXXXXXNSWNCGQEGEFASLSVKRLRKRQMRNFFLCLMVSQGVPMIYMGDEYGHTKGG 738
                    NSWNCGQEGEF S SVK+LRKRQMRN FL LMVSQGVPMIYMGDEYGHTKGG
Sbjct: 568  NNDGENHNNSWNCGQEGEFVSTSVKKLRKRQMRNLFLSLMVSQGVPMIYMGDEYGHTKGG 627

Query: 737  NNNTYCHDNYINYFRWDKKEESSAEFFRFCCLMTKFRQECEALGLDDFPTADRLQWHGHA 558
            NNNTYCHDNY+NYF+WDKKEESS++FFRFCCL+TKFRQECE+LGLDDFPT++RLQWHGH 
Sbjct: 628  NNNTYCHDNYLNYFQWDKKEESSSDFFRFCCLVTKFRQECESLGLDDFPTSERLQWHGHF 687

Query: 557  PGVPDWSEISRFVAFSL 507
            PG+PDWSE SRFVAF+L
Sbjct: 688  PGMPDWSETSRFVAFTL 704



 Score =  148 bits (374), Expect = 7e-33
 Identities = 69/87 (79%), Positives = 76/87 (87%)
 Frame = -3

Query: 457 MDSVKGEIYIAFNTSHLPVTITLPERPGYRWEPLVDTSKPAPYDFLSDDLPEKATATKQY 278
           +DSVKGEIY+AFN SHLP TITLPERPGYRWEPLVDTSKP P+DFL+ DLP +  A +QY
Sbjct: 705 VDSVKGEIYVAFNMSHLPFTITLPERPGYRWEPLVDTSKPIPFDFLTPDLPGRDIAIQQY 764

Query: 277 SHFLDANMYPMLSYSSIILLLAPDDNA 197
           +HFLDANMYPMLSYSSIILL  PD NA
Sbjct: 765 AHFLDANMYPMLSYSSIILLRTPDQNA 791


>gb|AAN15317.1| isoamylase isoform 1 [Solanum tuberosum]
          Length = 793

 Score = 1133 bits (2931), Expect = 0.0
 Identities = 536/697 (76%), Positives = 591/697 (84%), Gaps = 11/697 (1%)
 Frame = -2

Query: 2564 SQFYPKIQ-QKRSSQRRSENSL----IMKKMKSFAINARDGGGSTEVAETA----VIEKP 2412
            S + PK+        RRS N +    I +K +S  +NA    G   V +TA    V+EKP
Sbjct: 10   STYKPKLSFHNHLFSRRSSNGVDFESIWRKSRSSVVNAAVDSGRGGVVKTAATAVVVEKP 69

Query: 2411 KLRK--FEVFDGSPLPFGATAKDGGVNFAIHSSGAVSATLCLISLSDLQENRVTKKIDLD 2238
               +  FEV  G PLPFGATA DGGVNFA+ S  A +ATLCLI+LSDL E RVT++I LD
Sbjct: 70   TTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSDLPEKRVTEQIFLD 129

Query: 2237 PLVNKTGDVWHAFLKGDFEGMLYGYTFDGKFSPEEGHCYDSSKILLDPYAKAVISREEFG 2058
            PL NKTGDVWH FLKGDFE MLYGY FDGKF PEEGH +DSS+I+LDPYAKA++SR E+G
Sbjct: 130  PLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYAKAIVSRGEYG 189

Query: 2057 ALGAEGNCWPQMGGMVPPADNEFDWEGDLPLMYPQRDLIIYEMHVRGFTRHDSSGTNFPG 1878
             LG E +CWP M GMVP A ++FDWEGDLPL +PQRDL+IYEMHVRGFT H+SS T +PG
Sbjct: 190  VLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFPQRDLVIYEMHVRGFTNHESSETKYPG 249

Query: 1877 TYRGVVEKLDHLKELGVNCIELMPFQEYNEMEYFSYNSVLGNYKLNFWGYSTINYFSPML 1698
            TY GVVEKLDHLKELGVNCIELMP  E+NE+EY+SYNSVLG+YK NFWGYST+N+FSPM 
Sbjct: 250  TYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYSTVNFFSPMG 309

Query: 1697 RYSSAGIRNCGQDAINEVKVLVREAHKRGIEVLMDVVFNHTAEGNEKGPILSFRGVDNSV 1518
            RYSSAG+ NCG  AINE K LV+EAHKRGIEV+MDVVFNHTAEGNE GPILSFRG+DNSV
Sbjct: 310  RYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPILSFRGIDNSV 369

Query: 1517 YYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDLASILTRSSS 1338
            +Y LAPKGE+YNYSGCGNTFNCN+P+VRQFIVDCLRYWV EMHVDGFRFDLASILTRSSS
Sbjct: 370  FYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCLRYWVTEMHVDGFRFDLASILTRSSS 429

Query: 1337 LWDAVNVYGNSIEGDLLTTGTPLGSPPLIDMISNDPILRRVKLIAEAWDTGGLYQVGMFP 1158
             W+AVNVYGNSI+GD++TTGTPL SPPLIDMISNDPILR VKLIAEAWD GGLYQVGMFP
Sbjct: 430  SWNAVNVYGNSIDGDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGMFP 489

Query: 1157 HWGIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFVCAHDG 978
            HWGIWSEWNGKYRD+VRQFIKGTDGFSGAFAECLCGSPNLYQ GGRKPWNSINFVCAHDG
Sbjct: 490  HWGIWSEWNGKYRDMVRQFIKGTDGFSGAFAECLCGSPNLYQKGGRKPWNSINFVCAHDG 549

Query: 977  FTLADLVTYNKKHNLAXXXXXXXXXXXXNSWNCGQEGEFASLSVKRLRKRQMRNFFLCLM 798
            FTLADLVTYN KHNLA            NSWNCG+EGEFAS+ VK+LRKRQMRNFFLCLM
Sbjct: 550  FTLADLVTYNNKHNLANGEDNKDGENHNNSWNCGEEGEFASIFVKKLRKRQMRNFFLCLM 609

Query: 797  VSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESSAEFFRFCCLMTKFRQEC 618
            VSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKK+ESS++F RFC LMTKFR EC
Sbjct: 610  VSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFLRFCGLMTKFRHEC 669

Query: 617  EALGLDDFPTADRLQWHGHAPGVPDWSEISRFVAFSL 507
            E+LGLD FPTA+RLQWHGH P  PDWSE SRFVAF+L
Sbjct: 670  ESLGLDGFPTAERLQWHGHTPRTPDWSETSRFVAFTL 706



 Score =  150 bits (379), Expect = 2e-33
 Identities = 68/87 (78%), Positives = 78/87 (89%)
 Frame = -3

Query: 457 MDSVKGEIYIAFNTSHLPVTITLPERPGYRWEPLVDTSKPAPYDFLSDDLPEKATATKQY 278
           +D VKGE+YIAFN SHLPVTITLP+RPGYRW+P VDT KPAP+DFL+DD+PE+ TA KQY
Sbjct: 707 VDKVKGELYIAFNASHLPVTITLPDRPGYRWQPFVDTGKPAPFDFLTDDVPERETAAKQY 766

Query: 277 SHFLDANMYPMLSYSSIILLLAPDDNA 197
           SHFLDAN YPMLSYSSIILLL+  D+A
Sbjct: 767 SHFLDANQYPMLSYSSIILLLSSADDA 793