BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00012859
         (810 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285453.1| PREDICTED: probable 3-beta-hydroxysteroid-De...   335   9e-90
gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]   308   9e-82
ref|XP_002512695.1| sterol isomerase, putative [Ricinus communis...   301   1e-79
ref|XP_002304628.1| predicted protein [Populus trichocarpa] gi|2...   298   1e-78
gb|AFK46718.1| unknown [Lotus japonicus]                              295   1e-77

>ref|XP_002285453.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 2 [Vitis vinifera]
           gi|225435456|ref|XP_002285450.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 1 [Vitis vinifera] gi|297746323|emb|CBI16379.3|
           unnamed protein product [Vitis vinifera]
          Length = 222

 Score =  335 bits (858), Expect = 9e-90
 Identities = 160/215 (74%), Positives = 182/215 (84%)
 Frame = +2

Query: 14  EKMESNHPYVPRDLQLPDFTPGVLSQSTILGVYGTAALLLVSLIWTLSGRFPKISKIDRL 193
           E    +HPYVP+DL+LP + P VLSQSTI+GVYG ++LL+VS+IW +SGRFPK SKIDRL
Sbjct: 2   EGAPQSHPYVPKDLKLPGYVPVVLSQSTIVGVYGLSSLLVVSVIWIISGRFPKTSKIDRL 61

Query: 194 LMCWWAFTGLTHIILEGYFAFSPQFYKEKTACFLAEVWKEYSKGDSRYAGRDAGVVSVEG 373
           LMCWWAFTGLTH ILEGYF FSP+FYK+KTAC+LAEVWKEYSKGDSRYA RDAGV++VEG
Sbjct: 62  LMCWWAFTGLTHAILEGYFVFSPEFYKDKTACYLAEVWKEYSKGDSRYAARDAGVIAVEG 121

Query: 374 LTAVLEGPASLLAVYAIAKMTSYRYILQIAISLGQLYGTAVYFITSFLDGDNFAASTXXX 553
           +TAVLEGPASLLA+Y IA   SY YILQ+ ISLGQLYGTAVYFITS+LDGDNFAAS    
Sbjct: 122 ITAVLEGPASLLALYTIATKRSYSYILQLTISLGQLYGTAVYFITSYLDGDNFAASAYYY 181

Query: 554 XXXXVLMNSFWVWIPTLIVIRSWKKICAAFQAEVQ 658
               V  N+FWV IP+LIVIR WKKICAAFQ + Q
Sbjct: 182 YAYYVFANAFWVVIPSLIVIRCWKKICAAFQVQGQ 216


>gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]
          Length = 217

 Score =  308 bits (789), Expect = 9e-82
 Identities = 152/214 (71%), Positives = 177/214 (82%)
 Frame = +2

Query: 32  HPYVPRDLQLPDFTPGVLSQSTILGVYGTAALLLVSLIWTLSGRFPKISKIDRLLMCWWA 211
           HPYVPRDLQLP + P  LSQSTIL VYG ++LL+VSL+W LSGR   ISK+DRLLMCWWA
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 212 FTGLTHIILEGYFAFSPQFYKEKTACFLAEVWKEYSKGDSRYAGRDAGVVSVEGLTAVLE 391
           FTGLTHIILEGYFAFSP+FYK+KT  +LAEVWKEYSKGDSRYAGRD+ +V+VEG+T+VLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSTIVAVEGITSVLE 122

Query: 392 GPASLLAVYAIAKMTSYRYILQIAISLGQLYGTAVYFITSFLDGDNFAASTXXXXXXXVL 571
           GP  LLAVYAIAK   Y YILQ AISLGQLYGT VYFIT++L+GDNF+AS        VL
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 572 MNSFWVWIPTLIVIRSWKKICAAFQAEVQYEKNR 673
            NSFW+ IP+LI IR WKKI +A Q++ Q +KN+
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQ-KKNK 215


>ref|XP_002512695.1| sterol isomerase, putative [Ricinus communis]
           gi|223548656|gb|EEF50147.1| sterol isomerase, putative
           [Ricinus communis]
          Length = 220

 Score =  301 bits (771), Expect = 1e-79
 Identities = 148/209 (70%), Positives = 171/209 (81%)
 Frame = +2

Query: 32  HPYVPRDLQLPDFTPGVLSQSTILGVYGTAALLLVSLIWTLSGRFPKISKIDRLLMCWWA 211
           HPYVP DL+L D+ P  LSQSTIL V+GT++L+LVSL+W +SG   K +KIDR LMCWWA
Sbjct: 6   HPYVPTDLKLEDYVPVFLSQSTILAVFGTSSLVLVSLVWFISGSSRKAAKIDRWLMCWWA 65

Query: 212 FTGLTHIILEGYFAFSPQFYKEKTACFLAEVWKEYSKGDSRYAGRDAGVVSVEGLTAVLE 391
           FTGLTHIILEGYF FSP+FYK+KT+ +LAEVWKEYSKGDSRYA RD+ VV+VEGLTAVLE
Sbjct: 66  FTGLTHIILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARDSAVVAVEGLTAVLE 125

Query: 392 GPASLLAVYAIAKMTSYRYILQIAISLGQLYGTAVYFITSFLDGDNFAASTXXXXXXXVL 571
           GPASLLAVYAIA   SY YILQ AISLGQLYGTAVYF+T +LDG N+A+S        + 
Sbjct: 126 GPASLLAVYAIAAGKSYSYILQFAISLGQLYGTAVYFLTPYLDGYNYASSPYYHYAYYIG 185

Query: 572 MNSFWVWIPTLIVIRSWKKICAAFQAEVQ 658
            N+ WV IP+LI IRSWKKICAAFQ + Q
Sbjct: 186 ANASWVVIPSLICIRSWKKICAAFQVQGQ 214


>ref|XP_002304628.1| predicted protein [Populus trichocarpa] gi|222842060|gb|EEE79607.1|
           predicted protein [Populus trichocarpa]
          Length = 220

 Score =  298 bits (762), Expect = 1e-78
 Identities = 147/216 (68%), Positives = 173/216 (80%)
 Frame = +2

Query: 32  HPYVPRDLQLPDFTPGVLSQSTILGVYGTAALLLVSLIWTLSGRFPKISKIDRLLMCWWA 211
           HPY PRDL+LP + P  L+QSTI+GVY   +LL+VSLIW LSGR  KI+K++RLLMCWWA
Sbjct: 6   HPYAPRDLKLPGYVPNFLTQSTIVGVYLLTSLLVVSLIWILSGRSRKITKMNRLLMCWWA 65

Query: 212 FTGLTHIILEGYFAFSPQFYKEKTACFLAEVWKEYSKGDSRYAGRDAGVVSVEGLTAVLE 391
           FTGLTH+ILEGYFAFSP+FYK+KTA +LAEVWKEYSKGDSRYA RDA  V+VEGLTAVLE
Sbjct: 66  FTGLTHLILEGYFAFSPEFYKDKTAHYLAEVWKEYSKGDSRYAARDAATVTVEGLTAVLE 125

Query: 392 GPASLLAVYAIAKMTSYRYILQIAISLGQLYGTAVYFITSFLDGDNFAASTXXXXXXXVL 571
           GPASLLAVYAIA   SY YILQ A+ LGQLYGTAVYF+T++L+GD+FA S        + 
Sbjct: 126 GPASLLAVYAIASGKSYSYILQFAVCLGQLYGTAVYFLTAYLEGDHFATSPYHYYVYYIG 185

Query: 572 MNSFWVWIPTLIVIRSWKKICAAFQAEVQYEKNRKT 679
            N+ WV IP+LI +R WKKIC+A Q  V  +K  KT
Sbjct: 186 ANASWVVIPSLIAMRCWKKICSAVQ--VHGQKRTKT 219


>gb|AFK46718.1| unknown [Lotus japonicus]
          Length = 225

 Score =  295 bits (754), Expect = 1e-77
 Identities = 140/212 (66%), Positives = 169/212 (79%)
 Frame = +2

Query: 32  HPYVPRDLQLPDFTPGVLSQSTILGVYGTAALLLVSLIWTLSGRFPKISKIDRLLMCWWA 211
           HPYVPRDL LPD+ P  LSQS+IL V+  + LL+VS IW  SGR PK SK+DR+LMCWWA
Sbjct: 10  HPYVPRDLHLPDYVPCFLSQSSILSVFAISTLLVVSFIWIFSGRLPKKSKVDRVLMCWWA 69

Query: 212 FTGLTHIILEGYFAFSPQFYKEKTACFLAEVWKEYSKGDSRYAGRDAGVVSVEGLTAVLE 391
           FTGLTHIILEGYF F+P+F+K+KT  +LAEVWKEYSKGDSRY GRDAGVV+VEGLTAV+E
Sbjct: 70  FTGLTHIILEGYFVFAPEFFKDKTGFYLAEVWKEYSKGDSRYVGRDAGVVTVEGLTAVIE 129

Query: 392 GPASLLAVYAIAKMTSYRYILQIAISLGQLYGTAVYFITSFLDGDNFAASTXXXXXXXVL 571
           GPASLLAVYAIA   SY Y+LQ AISLGQLYGTAVY+IT+ L+GDNF+ ++       + 
Sbjct: 130 GPASLLAVYAIATGKSYSYMLQFAISLGQLYGTAVYYITAILEGDNFSTNSFYYYAYYIG 189

Query: 572 MNSFWVWIPTLIVIRSWKKICAAFQAEVQYEK 667
            N  W+ IP++I IR W+K  AAF+ + Q +K
Sbjct: 190 ANFSWIVIPSIIAIRCWRKTSAAFRVQGQTKK 221