BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00008807
         (2059 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267415.1| PREDICTED: NEDD8-activating enzyme E1 regula...   741   0.0  
ref|XP_004151890.1| PREDICTED: NEDD8-activating enzyme E1 regula...   737   0.0  
ref|XP_002524186.1| NEDD8-activating enzyme E1 regulatory subuni...   721   0.0  
gb|AFK44465.1| unknown [Lotus japonicus]                              715   0.0  
ref|XP_002320462.1| predicted protein [Populus trichocarpa] gi|2...   711   0.0  

>ref|XP_002267415.1| PREDICTED: NEDD8-activating enzyme E1 regulatory subunit [Vitis
            vinifera] gi|297739147|emb|CBI28798.3| unnamed protein
            product [Vitis vinifera]
          Length = 523

 Score =  741 bits (1912), Expect(2) = 0.0
 Identities = 364/436 (83%), Positives = 403/436 (92%)
 Frame = +3

Query: 69   MAEPKTKYDRQLRIWGEQGQSALEKSSICLLSCGPTGSETLKNLVLGGIGSITVVDGSKV 248
            MAEPKTKYDRQLRIWGEQGQ ALEK+SICLL+CGPTGSETLKNLVLGGIGSITV+DGSKV
Sbjct: 1    MAEPKTKYDRQLRIWGEQGQEALEKASICLLNCGPTGSETLKNLVLGGIGSITVIDGSKV 60

Query: 249  EEGDLGNNFMLDESSVGQSKAKSVCAFLQELNDAVKAKFVEESPDAIIETNPSFFSQFTL 428
            E GDLGNNFM+DESS+GQSKAK VCA LQELNDAVKAKF+EE P+A+IETNPSFFSQFTL
Sbjct: 61   ELGDLGNNFMVDESSIGQSKAKCVCASLQELNDAVKAKFIEEYPEALIETNPSFFSQFTL 120

Query: 429  VIATQLVEASVLKLDKICREANVMLICARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 608
            VIATQLVE S++KLD+ICREANVMLI ARSYGLTG VRISLKEH VIESKPDHFLDDLRL
Sbjct: 121  VIATQLVEDSMIKLDRICREANVMLIFARSYGLTGFVRISLKEHAVIESKPDHFLDDLRL 180

Query: 609  NNPWPELKSFAESVDLNATDPVVHKHTPYVVILVKMAEEWANSHGGCMPSTREEKREFKD 788
            NNPWPEL+ FAE++DLN +DPVVHKHTPYVVILVKMAE+W  SH G +PSTREEK+EFKD
Sbjct: 181  NNPWPELRGFAETIDLNVSDPVVHKHTPYVVILVKMAEQWTKSHDGKLPSTREEKKEFKD 240

Query: 789  LLKERMIALDEDNYKEAIEASFKISASRGISSGLRQIINDSAAEVDSTSSDFWVMVAALK 968
            LLK +MIA+DEDNYKEAIEASFK+ A RGISS L+QII+DS A+VDS+SS+FWVMVA+LK
Sbjct: 241  LLKSKMIAMDEDNYKEAIEASFKVFAPRGISSNLQQIIDDSRADVDSSSSNFWVMVASLK 300

Query: 969  EFIANEGGGEAPLEGSIPDMTSSTEYYVNLQKIYQAKAEVDILAVEERVRNILKRIGRDP 1148
            EFIANEGGGEAPLEGSIPDMTSSTE+YVNLQKIYQAKAE D L +E+RVRNILK+IGRDP
Sbjct: 301  EFIANEGGGEAPLEGSIPDMTSSTEHYVNLQKIYQAKAEADFLVIEQRVRNILKKIGRDP 360

Query: 1149 NAIPKANIKSFCRNARKLTVCKYQPIEDEINSPVLSELQKYLTDEDYSVAVGFYILLRAV 1328
            ++I KANIKSF +NARKLTVC+Y+ +E+E NSP+  ELQKYLTDEDYSVAVGFYILLRAV
Sbjct: 361  DSISKANIKSFSKNARKLTVCRYRLLEEEFNSPIQPELQKYLTDEDYSVAVGFYILLRAV 420

Query: 1329 DRFTATYNRFPGLFDG 1376
            DRF A YN FPG FDG
Sbjct: 421  DRFAANYNSFPGQFDG 436



 Score =  157 bits (397), Expect(2) = 0.0
 Identities = 77/86 (89%), Positives = 82/86 (95%)
 Frame = +1

Query: 1459 MDEDISRLKTIAVGMLSDLGCNGSTLSEDLINEMCRYGGAELHAVAAFIGGVASQEVIKL 1638
            MDEDISRLKT AV +LSDLGCNGSTL+EDLINEMCR+G AELHAVAAFIGG+ASQEVIKL
Sbjct: 438  MDEDISRLKTTAVSLLSDLGCNGSTLTEDLINEMCRFGAAELHAVAAFIGGIASQEVIKL 497

Query: 1639 ITKQFVPMSGTFIFNGIDHKCQMLSL 1716
            ITKQFVPMSGTFIFNGIDHK Q+LSL
Sbjct: 498  ITKQFVPMSGTFIFNGIDHKSQLLSL 523


>ref|XP_004151890.1| PREDICTED: NEDD8-activating enzyme E1 regulatory subunit-like
            [Cucumis sativus] gi|449523113|ref|XP_004168569.1|
            PREDICTED: NEDD8-activating enzyme E1 regulatory
            subunit-like [Cucumis sativus]
          Length = 523

 Score =  737 bits (1903), Expect(2) = 0.0
 Identities = 354/436 (81%), Positives = 398/436 (91%)
 Frame = +3

Query: 69   MAEPKTKYDRQLRIWGEQGQSALEKSSICLLSCGPTGSETLKNLVLGGIGSITVVDGSKV 248
            MAEPK KYDRQLRIWG+QGQ+ALEK+SICLL+CGPTGSETLKNLVLGGIGSIT+VDG+K+
Sbjct: 1    MAEPKVKYDRQLRIWGDQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60

Query: 249  EEGDLGNNFMLDESSVGQSKAKSVCAFLQELNDAVKAKFVEESPDAIIETNPSFFSQFTL 428
            EEGDLGNNFM+DESS+GQSKAK VCAFLQELNDAVKAKF+EE P+A+IET PSFFSQFTL
Sbjct: 61   EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEEYPEALIETRPSFFSQFTL 120

Query: 429  VIATQLVEASVLKLDKICREANVMLICARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 608
            V+ATQLVE  ++KLDKICR ANV+L+ ARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121  VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 609  NNPWPELKSFAESVDLNATDPVVHKHTPYVVILVKMAEEWANSHGGCMPSTREEKREFKD 788
            NNPWPEL+ FAE++DLNA DPV HKHTPYVVILVKMAEEWA SHGG +PS+REEK+ FKD
Sbjct: 181  NNPWPELRRFAETIDLNAPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKAFKD 240

Query: 789  LLKERMIALDEDNYKEAIEASFKISASRGISSGLRQIINDSAAEVDSTSSDFWVMVAALK 968
            LLK +MIA+DEDNYKEAIEASFK+ A RGISS L+QI+NDS AEVDS SSDFW++VAALK
Sbjct: 241  LLKAKMIAMDEDNYKEAIEASFKVFAPRGISSDLKQIVNDSCAEVDSNSSDFWILVAALK 300

Query: 969  EFIANEGGGEAPLEGSIPDMTSSTEYYVNLQKIYQAKAEVDILAVEERVRNILKRIGRDP 1148
            EFI NEGGGEAP+EGSIPDMTSSTE+YVNLQ IYQAKAE D   +EER RNILK+IGRDP
Sbjct: 301  EFIVNEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFKIIEERARNILKKIGRDP 360

Query: 1149 NAIPKANIKSFCRNARKLTVCKYQPIEDEINSPVLSELQKYLTDEDYSVAVGFYILLRAV 1328
            N+I K  +KSFC+NARKL VC+Y+ +EDE NSP++ ELQKYLTDED+SVAVGFY+LLRAV
Sbjct: 361  NSISKTTVKSFCKNARKLRVCRYRSLEDEFNSPIVPELQKYLTDEDFSVAVGFYLLLRAV 420

Query: 1329 DRFTATYNRFPGLFDG 1376
            DRF A YN FPG FDG
Sbjct: 421  DRFAANYNSFPGQFDG 436



 Score =  152 bits (383), Expect(2) = 0.0
 Identities = 73/86 (84%), Positives = 80/86 (93%)
 Frame = +1

Query: 1459 MDEDISRLKTIAVGMLSDLGCNGSTLSEDLINEMCRYGGAELHAVAAFIGGVASQEVIKL 1638
            +DEDISRLKT AVG+LSDLGCNG TLSEDLINEMCR+G AELH VAAF GG+ASQEVIKL
Sbjct: 438  IDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHVVAAFTGGIASQEVIKL 497

Query: 1639 ITKQFVPMSGTFIFNGIDHKCQMLSL 1716
            IT+QFVPMSGTF+FNGIDHK Q+LSL
Sbjct: 498  ITRQFVPMSGTFVFNGIDHKSQLLSL 523


>ref|XP_002524186.1| NEDD8-activating enzyme E1 regulatory subunit, putative [Ricinus
            communis] gi|223536555|gb|EEF38201.1| NEDD8-activating
            enzyme E1 regulatory subunit, putative [Ricinus communis]
          Length = 523

 Score =  721 bits (1860), Expect(2) = 0.0
 Identities = 351/436 (80%), Positives = 396/436 (90%)
 Frame = +3

Query: 69   MAEPKTKYDRQLRIWGEQGQSALEKSSICLLSCGPTGSETLKNLVLGGIGSITVVDGSKV 248
            MAE KTKYDRQLRIWGEQGQ+ALEK+SICLL+CGPTGSETLKNLVLGGIGSITV+DGSKV
Sbjct: 1    MAEAKTKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITVIDGSKV 60

Query: 249  EEGDLGNNFMLDESSVGQSKAKSVCAFLQELNDAVKAKFVEESPDAIIETNPSFFSQFTL 428
            E GDLGNNFM+DESSVGQ KAK VCAFLQELNDAVKAKF+EE P+A+IETNPSFFSQFTL
Sbjct: 61   ETGDLGNNFMVDESSVGQPKAKCVCAFLQELNDAVKAKFIEEHPEALIETNPSFFSQFTL 120

Query: 429  VIATQLVEASVLKLDKICREANVMLICARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 608
            V+ATQLVE S++KLD+ICREANVMLI ARSYGL G VRIS+KEHTVIESKPDHFLDDLRL
Sbjct: 121  VVATQLVEDSMVKLDRICREANVMLIFARSYGLAGFVRISVKEHTVIESKPDHFLDDLRL 180

Query: 609  NNPWPELKSFAESVDLNATDPVVHKHTPYVVILVKMAEEWANSHGGCMPSTREEKREFKD 788
            NNPWPELKSFAE++DLN  DPV HKHTPY++ILVKMA+EWA +HGG +PS+R+EK++FK+
Sbjct: 181  NNPWPELKSFAETIDLNVADPVAHKHTPYIIILVKMADEWAKAHGGSLPSSRDEKKQFKE 240

Query: 789  LLKERMIALDEDNYKEAIEASFKISASRGISSGLRQIINDSAAEVDSTSSDFWVMVAALK 968
            L+K  MIALDEDNY+EA EASFK+ A RGIS  L+QII+D+ A++DS+SS+FWVMVAALK
Sbjct: 241  LIKAGMIALDEDNYREATEASFKVFAPRGISPDLQQIISDNRADLDSSSSNFWVMVAALK 300

Query: 969  EFIANEGGGEAPLEGSIPDMTSSTEYYVNLQKIYQAKAEVDILAVEERVRNILKRIGRDP 1148
            EFI NEG GEAPLEGSIPDMTSSTE YVNLQKIYQAKAE D L +E+RVRNILK+IGRDP
Sbjct: 301  EFIVNEGDGEAPLEGSIPDMTSSTELYVNLQKIYQAKAEADFLVIEKRVRNILKKIGRDP 360

Query: 1149 NAIPKANIKSFCRNARKLTVCKYQPIEDEINSPVLSELQKYLTDEDYSVAVGFYILLRAV 1328
            N+I KA IK FC+NARKL VC+Y+ IEDE N+P L +LQKYLTDEDYSVA+GFYILLRAV
Sbjct: 361  NSISKAMIKCFCKNARKLKVCRYRLIEDEFNNPSLPQLQKYLTDEDYSVAMGFYILLRAV 420

Query: 1329 DRFTATYNRFPGLFDG 1376
            DRF A YN FPG FDG
Sbjct: 421  DRFAANYNSFPGQFDG 436



 Score =  148 bits (374), Expect(2) = 0.0
 Identities = 72/86 (83%), Positives = 80/86 (93%)
 Frame = +1

Query: 1459 MDEDISRLKTIAVGMLSDLGCNGSTLSEDLINEMCRYGGAELHAVAAFIGGVASQEVIKL 1638
            MDEDISRLKT AV +LSDLGCNGS L+EDLINEMCR+G +ELHAVAAFIGGVASQEVIKL
Sbjct: 438  MDEDISRLKTTAVSLLSDLGCNGSPLTEDLINEMCRFGASELHAVAAFIGGVASQEVIKL 497

Query: 1639 ITKQFVPMSGTFIFNGIDHKCQMLSL 1716
            IT+QFVP+ GTFIFNGIDHK Q+L+L
Sbjct: 498  ITRQFVPIPGTFIFNGIDHKSQLLAL 523


>gb|AFK44465.1| unknown [Lotus japonicus]
          Length = 523

 Score =  715 bits (1845), Expect(2) = 0.0
 Identities = 354/435 (81%), Positives = 387/435 (88%)
 Frame = +3

Query: 69   MAEPKTKYDRQLRIWGEQGQSALEKSSICLLSCGPTGSETLKNLVLGGIGSITVVDGSKV 248
            MAEPK KYDRQLRIWGEQGQ+ALEKSSICLL+CGPTGSETLKNLVLGG+GSITVVDGSKV
Sbjct: 1    MAEPKVKYDRQLRIWGEQGQAALEKSSICLLNCGPTGSETLKNLVLGGVGSITVVDGSKV 60

Query: 249  EEGDLGNNFMLDESSVGQSKAKSVCAFLQELNDAVKAKFVEESPDAIIETNPSFFSQFTL 428
            E GDLGNNF++DESS+GQSKAK VC+FLQELNDAVKAKFVEE P+ +IETNPSFFSQFTL
Sbjct: 61   EVGDLGNNFLVDESSLGQSKAKCVCSFLQELNDAVKAKFVEEYPETLIETNPSFFSQFTL 120

Query: 429  VIATQLVEASVLKLDKICREANVMLICARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 608
            VIATQLVE S +KLD+ICREANVMLI ARSYGLTG VRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121  VIATQLVENSTIKLDQICREANVMLIFARSYGLTGFVRISLKEHTVIESKPDHFLDDLRL 180

Query: 609  NNPWPELKSFAESVDLNATDPVVHKHTPYVVILVKMAEEWANSHGGCMPSTREEKREFKD 788
            NNPWPELK FAE  DLN  DPV HKH PYVVILVKMA+EWA SHGG +PSTREEK+EFK+
Sbjct: 181  NNPWPELKRFAEGFDLNLQDPVAHKHIPYVVILVKMADEWAKSHGGRLPSTREEKKEFKE 240

Query: 789  LLKERMIALDEDNYKEAIEASFKISASRGISSGLRQIINDSAAEVDSTSSDFWVMVAALK 968
            LLK  M+A DEDNYKEAIE+SFK+ A RGIS  L+QI+NDS+AEVDS SSDFWVMVAALK
Sbjct: 241  LLKAGMVAQDEDNYKEAIESSFKVFAPRGISLELQQILNDSSAEVDSNSSDFWVMVAALK 300

Query: 969  EFIANEGGGEAPLEGSIPDMTSSTEYYVNLQKIYQAKAEVDILAVEERVRNILKRIGRDP 1148
            +FI NEGGGE PLEGSIPDMTSSTE YVNLQ IYQAK+E D L +E  V++ LK+IGRD 
Sbjct: 301  DFITNEGGGETPLEGSIPDMTSSTEQYVNLQNIYQAKSEADFLVIERLVKSTLKKIGRDS 360

Query: 1149 NAIPKANIKSFCRNARKLTVCKYQPIEDEINSPVLSELQKYLTDEDYSVAVGFYILLRAV 1328
            N+IP+A IKSFC+NARKL VC+Y+ IEDE N P LSELQKYLTDEDYS+AVG YILLRAV
Sbjct: 361  NSIPRATIKSFCKNARKLKVCRYRLIEDEFNFPNLSELQKYLTDEDYSIAVGIYILLRAV 420

Query: 1329 DRFTATYNRFPGLFD 1373
            DRF A YN FPG FD
Sbjct: 421  DRFAANYNSFPGQFD 435



 Score =  151 bits (381), Expect(2) = 0.0
 Identities = 71/86 (82%), Positives = 82/86 (95%)
 Frame = +1

Query: 1459 MDEDISRLKTIAVGMLSDLGCNGSTLSEDLINEMCRYGGAELHAVAAFIGGVASQEVIKL 1638
            MDEDISRLK+ A+G+L+DLGCNG+TL+EDLINEMCR+G AELHAVAA +GG+ASQEVIKL
Sbjct: 438  MDEDISRLKSTAIGLLNDLGCNGATLAEDLINEMCRFGAAELHAVAALVGGIASQEVIKL 497

Query: 1639 ITKQFVPMSGTFIFNGIDHKCQMLSL 1716
            IT+QFVPMSGTFIFNGIDHK Q+LSL
Sbjct: 498  ITRQFVPMSGTFIFNGIDHKSQILSL 523


>ref|XP_002320462.1| predicted protein [Populus trichocarpa] gi|222861235|gb|EEE98777.1|
            predicted protein [Populus trichocarpa]
          Length = 521

 Score =  711 bits (1834), Expect(2) = 0.0
 Identities = 345/436 (79%), Positives = 395/436 (90%)
 Frame = +3

Query: 69   MAEPKTKYDRQLRIWGEQGQSALEKSSICLLSCGPTGSETLKNLVLGGIGSITVVDGSKV 248
            MAEPKTKYDRQLRIWGEQGQ+ALEK++ICLL+CGPTGSETLKNLVLGG+GSITV+DGSKV
Sbjct: 1    MAEPKTKYDRQLRIWGEQGQAALEKATICLLNCGPTGSETLKNLVLGGVGSITVIDGSKV 60

Query: 249  EEGDLGNNFMLDESSVGQSKAKSVCAFLQELNDAVKAKFVEESPDAIIETNPSFFSQFTL 428
            E GDLGNNFM+DES VGQSKAK VC FLQELNDAVKAKF+EE P+A+I +NPSFFSQFTL
Sbjct: 61   ELGDLGNNFMVDESCVGQSKAKCVCTFLQELNDAVKAKFIEEYPEALIGSNPSFFSQFTL 120

Query: 429  VIATQLVEASVLKLDKICREANVMLICARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 608
            V+ATQL E S++KLDKICREANV+LI ARSYGLTG VRIS+KEH VIESKPDHFLDDLRL
Sbjct: 121  VVATQLAEDSMIKLDKICREANVLLIFARSYGLTGFVRISVKEHAVIESKPDHFLDDLRL 180

Query: 609  NNPWPELKSFAESVDLNATDPVVHKHTPYVVILVKMAEEWANSHGGCMPSTREEKREFKD 788
            NNPWPELK FAE++DLN TDPV HKHTPYVVILVK+AEEW  +HGG +PSTR+EK+EFK+
Sbjct: 181  NNPWPELKRFAETIDLNVTDPVAHKHTPYVVILVKIAEEWTKAHGGALPSTRDEKKEFKE 240

Query: 789  LLKERMIALDEDNYKEAIEASFKISASRGISSGLRQIINDSAAEVDSTSSDFWVMVAALK 968
            LLK  M+A+DEDNYKEAIEASFK+ A RGIS    +II+DS +EV+S SSDFWVMVAALK
Sbjct: 241  LLKAGMVAMDEDNYKEAIEASFKVFAPRGISE--YKIIHDSCSEVESNSSDFWVMVAALK 298

Query: 969  EFIANEGGGEAPLEGSIPDMTSSTEYYVNLQKIYQAKAEVDILAVEERVRNILKRIGRDP 1148
            EFIANEGG EAPLEGSIPDMTSSTE YVNLQKIYQAKAE D LA+++RV++ILKR+GRDP
Sbjct: 299  EFIANEGGEEAPLEGSIPDMTSSTELYVNLQKIYQAKAEADFLAIQQRVKSILKRMGRDP 358

Query: 1149 NAIPKANIKSFCRNARKLTVCKYQPIEDEINSPVLSELQKYLTDEDYSVAVGFYILLRAV 1328
            ++I K  IKSFC+NARKL +C+Y+PIEDE N+P ++ELQKYLTDE+YSVA+GFYILLR+V
Sbjct: 359  DSISKEMIKSFCKNARKLKICRYRPIEDEFNNPAVTELQKYLTDEEYSVAMGFYILLRSV 418

Query: 1329 DRFTATYNRFPGLFDG 1376
            DRF A YN FPG F+G
Sbjct: 419  DRFAANYNSFPGQFEG 434



 Score =  143 bits (361), Expect(2) = 0.0
 Identities = 69/87 (79%), Positives = 79/87 (90%)
 Frame = +1

Query: 1456 EMDEDISRLKTIAVGMLSDLGCNGSTLSEDLINEMCRYGGAELHAVAAFIGGVASQEVIK 1635
            EMD DISRLKT  VG+LSDLGCNG+T++EDLINEMCR+G +ELHAVAAFIGG+ASQEVIK
Sbjct: 435  EMDGDISRLKTTVVGLLSDLGCNGATVTEDLINEMCRFGASELHAVAAFIGGIASQEVIK 494

Query: 1636 LITKQFVPMSGTFIFNGIDHKCQMLSL 1716
             ITKQFVPM GTFIFNGID + Q+L+L
Sbjct: 495  PITKQFVPMVGTFIFNGIDQRSQLLAL 521