BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00008426
         (1418 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_191460.1| pectinesterase 35 [Arabidopsis thaliana] gi|753...   226   8e-69
ref|XP_002878254.1| pectinesterase family protein [Arabidopsis l...   221   6e-67
ref|XP_003632058.1| PREDICTED: probable pectinesterase/pectinest...   244   3e-62
emb|CBI39447.3| unnamed protein product [Vitis vinifera]              230   6e-58
ref|XP_002527752.1| Pectinesterase-2 precursor, putative [Ricinu...   230   8e-58

>ref|NP_191460.1| pectinesterase 35 [Arabidopsis thaliana]
            gi|75311708|sp|Q9LYT5.1|PME35_ARATH RecName:
            Full=Probable pectinesterase/pectinesterase inhibitor 35;
            Includes: RecName: Full=Pectinesterase inhibitor 35;
            AltName: Full=Pectin methylesterase inhibitor 35;
            Includes: RecName: Full=Pectinesterase 35; Short=PE 35;
            AltName: Full=Pectin methylesterase 35; Short=AtPME35;
            Flags: Precursor gi|7529744|emb|CAB86929.1|
            pectinesterase precursor-like protein [Arabidopsis
            thaliana] gi|16974625|gb|AAL31215.1| AT3g59010/F17J16_60
            [Arabidopsis thaliana] gi|24111417|gb|AAN46858.1|
            At3g59010/F17J16_60 [Arabidopsis thaliana]
            gi|332646339|gb|AEE79860.1| pectinesterase 35
            [Arabidopsis thaliana]
          Length = 529

 Score =  226 bits (577), Expect(2) = 8e-69
 Identities = 131/228 (57%), Positives = 154/228 (67%), Gaps = 1/228 (0%)
 Frame = +2

Query: 737  KQKYTIDVDLQNNIHHISNTLALHKSIVTEEAKTLKVGSGGRKLLSD-DFPTWVLPSERK 913
            K+   ID   +N    ++N+L +  S   + + +  + +GGRKLLSD DFPTWV  S+RK
Sbjct: 158  KEGIAIDSFARNLTGLLTNSLDMFVSDKQKSSSSSNL-TGGRKLLSDHDFPTWVSSSDRK 216

Query: 914  LLEASIEGIEADAVVATDGTGTHKSIAEALAFVSLAGTGGGRTVIQVKAGTYNENNLKIP 1093
            LLEAS+E +   AVVA DG+GTH S+AEALA +     G GR+VI + AGTY EN L IP
Sbjct: 217  LLEASVEELRPHAVVAADGSGTHMSVAEALASLE---KGSGRSVIHLTAGTYKEN-LNIP 272

Query: 1094 VKQSNVMLVGDGKGKTVIXXXXXXXXXXXXXXXXXXXISGDGFIARDITFQNTAGAGNHQ 1273
             KQ NVMLVGDGKGKTVI                     GDGFIARDITF N+AG  + Q
Sbjct: 273  SKQKNVMLVGDGKGKTVIVGSRSNRGGWNTYQSATVAAMGDGFIARDITFVNSAGPNSEQ 332

Query: 1274 AVALRVSSDKSVFYRCSIIGYQDTLYTLSNRQFYRETDIYGTVDFIFG 1417
            AVALRV SD+SV YRCSI GYQD+LYTLS RQFYRETDI GTVDFIFG
Sbjct: 333  AVALRVGSDRSVVYRCSIDGYQDSLYTLSKRQFYRETDITGTVDFIFG 380



 Score = 62.0 bits (149), Expect(2) = 8e-69
 Identities = 39/128 (30%), Positives = 70/128 (54%)
 Frame = +1

Query: 250 STFSRASTTSPCAHTLYPEACESLILSTKDGSTPTTLGDMFDLSIKFTIDQAQSIRSLAY 429
           S F+ +S +     + +P +  S   S    ST +  G  F  S++ ++D A   RSLA+
Sbjct: 31  SVFAHSSNSKFTKISRHPNSDSS---SRTKPSTSSNKG--FLSSVQLSLDHALFARSLAF 85

Query: 430 NVSRMNEKPNSNSLTVLDDCVELLDHSLHQLAKILDDHKATLSSEDIQTWLSSAITHQTT 609
           N++  +    +  L  ++DC+ELLD +L  L +I+   +    ++D+ TWLS+A+T+Q T
Sbjct: 86  NLTLSHRTSQTLMLDPVNDCLELLDDTLDMLYRIVVIKRKDHVNDDVHTWLSAALTNQET 145

Query: 610 CLDTLQSQ 633
           C  +L  +
Sbjct: 146 CKQSLSEK 153


>ref|XP_002878254.1| pectinesterase family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297324092|gb|EFH54513.1| pectinesterase family protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 525

 Score =  221 bits (564), Expect(2) = 6e-67
 Identities = 129/228 (56%), Positives = 153/228 (67%), Gaps = 1/228 (0%)
 Frame = +2

Query: 737  KQKYTIDVDLQNNIHHISNTLALHKSIVTEEAKTLKVGSGGRKLLSD-DFPTWVLPSERK 913
            K    ID   +N    ++N+L +  S   + + + ++ +GGRKLLS+ DFP+W   S+RK
Sbjct: 154  KDGIAIDSFARNLTGLLTNSLDMFVSDKRKSSSSSRL-TGGRKLLSEHDFPSWFSMSDRK 212

Query: 914  LLEASIEGIEADAVVATDGTGTHKSIAEALAFVSLAGTGGGRTVIQVKAGTYNENNLKIP 1093
            LLEAS+E +   AVVA DG+GTH SIAEALA +     G GR+VI + AGTY EN L IP
Sbjct: 213  LLEASVEELRPHAVVAADGSGTHMSIAEALASLE---KGSGRSVIHLAAGTYKEN-LNIP 268

Query: 1094 VKQSNVMLVGDGKGKTVIXXXXXXXXXXXXXXXXXXXISGDGFIARDITFQNTAGAGNHQ 1273
             KQ NVMLVGDGKGKTVI                     GDGFIARDITF N+AG  + Q
Sbjct: 269  SKQKNVMLVGDGKGKTVIIGSRSNRGGWNTYQSATVAAMGDGFIARDITFVNSAGPNSEQ 328

Query: 1274 AVALRVSSDKSVFYRCSIIGYQDTLYTLSNRQFYRETDIYGTVDFIFG 1417
            AVALRV SD+SV YRCSI GYQD+LYTLS RQFYRETDI GTVDFIFG
Sbjct: 329  AVALRVGSDRSVVYRCSIDGYQDSLYTLSKRQFYRETDITGTVDFIFG 376



 Score = 60.8 bits (146), Expect(2) = 6e-67
 Identities = 38/129 (29%), Positives = 70/129 (54%)
 Frame = +1

Query: 247 ISTFSRASTTSPCAHTLYPEACESLILSTKDGSTPTTLGDMFDLSIKFTIDQAQSIRSLA 426
           +S F+ +S +     +  P +  S   S    ST +  G  F  S++ +++ A   RS A
Sbjct: 26  LSVFAHSSNSKFTKFSRNPNSDSS---SRTKPSTSSNKG--FLNSVQLSLEDALFARSFA 80

Query: 427 YNVSRMNEKPNSNSLTVLDDCVELLDHSLHQLAKILDDHKATLSSEDIQTWLSSAITHQT 606
           +N++  +    S  L  ++DC+ELLD +L  L++I+   +    ++D+ TWLS+A+T+Q 
Sbjct: 81  FNLTLSHRTSQSLMLDPVNDCLELLDDTLDMLSRIVVIKRKDHVNDDVHTWLSAALTNQE 140

Query: 607 TCLDTLQSQ 633
           TC  +L  +
Sbjct: 141 TCKQSLSEK 149


>ref|XP_003632058.1| PREDICTED: probable pectinesterase/pectinesterase inhibitor 35-like
            [Vitis vinifera]
          Length = 553

 Score =  244 bits (624), Expect = 3e-62
 Identities = 138/218 (63%), Positives = 154/218 (70%), Gaps = 1/218 (0%)
 Frame = +2

Query: 767  QNNIHHISNTLALHKSI-VTEEAKTLKVGSGGRKLLSDDFPTWVLPSERKLLEASIEGIE 943
            QN  + ISN+LALH S   ++EA+        R+LLSD FP WV  +ERKLLEAS+E I 
Sbjct: 188  QNLTYSISNSLALHMSTRPSKEAQRTNTAGHHRRLLSDRFPGWVTAAERKLLEASVEEIG 247

Query: 944  ADAVVATDGTGTHKSIAEALAFVSLAGTGGGRTVIQVKAGTYNENNLKIPVKQSNVMLVG 1123
            A AVVA DG+GTHK+I EALA V +   G GRTVI VKAGTY+E  LKIP  Q NVMLVG
Sbjct: 248  ATAVVAKDGSGTHKTIGEALAMV-VTLEGEGRTVIHVKAGTYDEG-LKIPSSQKNVMLVG 305

Query: 1124 DGKGKTVIXXXXXXXXXXXXXXXXXXXISGDGFIARDITFQNTAGAGNHQAVALRVSSDK 1303
            DGKGKTVI                   + GDGFIARDIT +N AG G  QAVALRV SD+
Sbjct: 306  DGKGKTVIVGHKSYAGGSSTYDSATVGVMGDGFIARDITIENDAGPGKGQAVALRVGSDR 365

Query: 1304 SVFYRCSIIGYQDTLYTLSNRQFYRETDIYGTVDFIFG 1417
            SV +RCSIIGYQDTLYTLS RQFYRETDIYGTVDFIFG
Sbjct: 366  SVVFRCSIIGYQDTLYTLSKRQFYRETDIYGTVDFIFG 403



 Score =  104 bits (259), Expect = 6e-20
 Identities = 52/135 (38%), Positives = 89/135 (65%), Gaps = 5/135 (3%)
 Frame = +1

Query: 241 SHISTFSRAS----TTSPCAHTLYPEACESLILSTKDGSTPTTLGDMFDLSIKFTIDQAQ 408
           SH ST  +AS     ++ C  T Y  AC+S +LS+ +G+ P T  D+FDLS++F+++QA+
Sbjct: 41  SHTSTVPKASMEAIVSAACKATFYQTACQSALLSSTNGAVPQTQADLFDLSVQFSLNQAR 100

Query: 409 SIRSLAYNVSRMNEKPNSNSLTVLDDCVELLDHSLHQLAKILDDHKATLSS-EDIQTWLS 585
           S R+  +++  ++ K  +  +   DDC+ELLD +L QL  + +  K  +   +D+QTWLS
Sbjct: 101 SARAHVHDLRLLDHK--TQIVRGTDDCMELLDDTLDQLTNVANRRKTLIEDPDDVQTWLS 158

Query: 586 SAITHQTTCLDTLQS 630
           +A+T+Q TCL+++Q+
Sbjct: 159 AALTNQATCLESIQT 173


>emb|CBI39447.3| unnamed protein product [Vitis vinifera]
          Length = 432

 Score =  230 bits (587), Expect = 6e-58
 Identities = 126/186 (67%), Positives = 137/186 (73%)
 Frame = +2

Query: 860  RKLLSDDFPTWVLPSERKLLEASIEGIEADAVVATDGTGTHKSIAEALAFVSLAGTGGGR 1039
            R+LLSD FP WV  +ERKLLEAS+E I A AVVA DG+GTHK+I EALA V +   G GR
Sbjct: 99   RRLLSDRFPGWVTAAERKLLEASVEEIGATAVVAKDGSGTHKTIGEALAMV-VTLEGEGR 157

Query: 1040 TVIQVKAGTYNENNLKIPVKQSNVMLVGDGKGKTVIXXXXXXXXXXXXXXXXXXXISGDG 1219
            TVI VKAGTY+E  LKIP  Q NVMLVGDGKGKTVI                   + GDG
Sbjct: 158  TVIHVKAGTYDEG-LKIPSSQKNVMLVGDGKGKTVIVGHKSYAGGSSTYDSATVGVMGDG 216

Query: 1220 FIARDITFQNTAGAGNHQAVALRVSSDKSVFYRCSIIGYQDTLYTLSNRQFYRETDIYGT 1399
            FIARDIT +N AG G  QAVALRV SD+SV +RCSIIGYQDTLYTLS RQFYRETDIYGT
Sbjct: 217  FIARDITIENDAGPGKGQAVALRVGSDRSVVFRCSIIGYQDTLYTLSKRQFYRETDIYGT 276

Query: 1400 VDFIFG 1417
            VDFIFG
Sbjct: 277  VDFIFG 282


>ref|XP_002527752.1| Pectinesterase-2 precursor, putative [Ricinus communis]
            gi|223532839|gb|EEF34613.1| Pectinesterase-2 precursor,
            putative [Ricinus communis]
          Length = 583

 Score =  230 bits (586), Expect = 8e-58
 Identities = 132/222 (59%), Positives = 153/222 (68%), Gaps = 5/222 (2%)
 Frame = +2

Query: 767  QNNIHHISNTLALHKSIVTEEAKTLKVGSGGRKLLS-DDFPTWVLPSERKLLEASIEGIE 943
            +N   HISN+LAL+ S       T +   GGRKLLS DDFP WV  SERKLLE  +  I+
Sbjct: 213  RNLSQHISNSLALYMS---HYYNTKESNKGGRKLLSGDDFPAWVSESERKLLETPVAEIK 269

Query: 944  ADAVVATDGTGTHKSIAEALAFV---SLAGTGGG-RTVIQVKAGTYNENNLKIPVKQSNV 1111
            A AVVA DG+GTH +I EA+A V   SLAG GGG R VI VKAGTY+EN LK+P KQ NV
Sbjct: 270  AHAVVAKDGSGTHTTIGEAIAEVMSYSLAGEGGGGRNVIYVKAGTYHEN-LKLPTKQKNV 328

Query: 1112 MLVGDGKGKTVIXXXXXXXXXXXXXXXXXXXISGDGFIARDITFQNTAGAGNHQAVALRV 1291
            MLVGDGKGK+VI                   + GDGF+A+ ITF N+AG   HQAVALRV
Sbjct: 329  MLVGDGKGKSVITGSRSADDGYSTFQTATVSVMGDGFMAKGITFVNSAGPNKHQAVALRV 388

Query: 1292 SSDKSVFYRCSIIGYQDTLYTLSNRQFYRETDIYGTVDFIFG 1417
            +SDKSV Y+CS+   QDTLYT S RQFYR+TDIYGT+DFIFG
Sbjct: 389  ASDKSVIYQCSLEANQDTLYTHSKRQFYRDTDIYGTIDFIFG 430



 Score = 84.7 bits (208), Expect = 5e-14
 Identities = 62/194 (31%), Positives = 97/194 (50%), Gaps = 25/194 (12%)
 Frame = +1

Query: 130 PLXSFKQERXXXXXXXXXXXXCFFIVVVSIFPT----QSKPSHISTFSRAST-------- 273
           P  SF   +              F+++++ F +       PS  S FSR+S+        
Sbjct: 5   PRISFSHAKCSSTRTCSFPLILIFLIIINFFCSAVVASPIPSQFSKFSRSSSPAEPKKSP 64

Query: 274 ---TSPCAHTLYPEACESLILSTKDGSTPTTLGDMFDLSIKFTIDQAQSIRSLAYNVSRM 444
              +S C    Y  ACES++ S K  + PTT  ++FD S+ +T+ QA+S   LA  VS  
Sbjct: 65  ITSSSACNDIPYKSACESVLSSLKT-TIPTTPKELFDHSVHYTLKQARSAFELASTVSPS 123

Query: 445 NE---KPNSNSLTV------LDDCVELLDHSLHQLAKILD-DHKATLSSEDIQTWLSSAI 594
           N      N++   V      + DCVELL+ SL  L+ I +  +    +  D+QTWLS+A+
Sbjct: 124 NNVKAAANTHLAAVAGGGGGMIDCVELLEDSLDLLSTITNRKNNPKHTHNDVQTWLSAAL 183

Query: 595 THQTTCLDTLQSQK 636
           T+Q TC+++LQ+ K
Sbjct: 184 TNQETCIESLQNDK 197