BLASTX nr result
ID: Cimicifuga21_contig00008360
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cimicifuga21_contig00008360
(2583 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004163508.1| PREDICTED: uncharacterized protein LOC101231... 515 e-143
ref|XP_004136436.1| PREDICTED: uncharacterized protein LOC101208... 514 e-143
emb|CBI20922.3| unnamed protein product [Vitis vinifera] 505 e-140
ref|XP_002516583.1| hypothetical protein RCOM_0803370 [Ricinus c... 484 e-134
ref|XP_002324251.1| predicted protein [Populus trichocarpa] gi|2... 474 e-131
>ref|XP_004163508.1| PREDICTED: uncharacterized protein LOC101231100 [Cucumis sativus]
Length = 600
Score = 515 bits (1327), Expect = e-143
Identities = 294/624 (47%), Positives = 385/624 (61%), Gaps = 8/624 (1%)
Frame = +3
Query: 276 EASEIEDCEFRWXXXXXXXXXXXEVQFYESFTYDGNEYSLYDNVYLYKEDETEPYIGKLV 455
E ++E+ EF+W +V FYESFTYDG EY LYD+VYLYKE E EPYIGKL+
Sbjct: 3 ETEQVENVEFQWGKKKGIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGKLL 62
Query: 456 KIWEQPQQKKKVKVLWFFRQIEILNWLEGVPTLENEIFLASGDGLGLANVNSLEAIAGKC 635
KIW+ P + KKVK+LWFFR EILN+L GV T ENE+FLASGDG+GLAN+NSLE +AGKC
Sbjct: 63 KIWQNPDKTKKVKILWFFRSCEILNYLGGVDTRENELFLASGDGIGLANINSLEVLAGKC 122
Query: 636 HVVCTSKDSRNPQPSQEQLKMADYIFYRTFDVDNYTVSDKINDKIAGVEVRFLLNKEGAQ 815
+V+C S DSRNPQPS E LK AD++F RTFDV V ++I DKIAGVEV+ LLNK A
Sbjct: 123 NVLCISNDSRNPQPSDEALKKADFVFCRTFDVGKQEVCNEICDKIAGVEVKLLLNK--AD 180
Query: 816 QSSGGLKFDSAMKDENGNVMASNEVSELCPKLNSPATLVNTMTDDMPGHPMKKK-DEDSN 992
S + D KD +G + + ++ + + S L T D K+ D +
Sbjct: 181 SSKDVKRTDKDGKDASGIAIVNTQLEDPSGRNISNGELTLTTNDSSLEKSTKENVDLKGS 240
Query: 993 I--SANQNPLPGEDLVSGLG-GETLETEVPTIMQEHVSLYQKTLPSPKSDSCKDEVNTGT 1163
I S+N+ + G+G G+T +I++ L K P K DS + N
Sbjct: 241 IEKSSNEEKSSAHAIERGIGMGKT-----SSIIKHENILGDKVPPKLKIDSNEKPGNAKD 295
Query: 1164 VCISKVGHKKLKSSDDLGVLDDRPSKKARFDTSIKLPEEQCISPSLEANKNKNGNSLQNK 1343
V + KS + ++ RP+KK + D+S++L + K N ++ K
Sbjct: 296 V------EGRAKSLRESAEVEHRPAKKTKLDSSVQL------------SPGKTENDIE-K 336
Query: 1344 QRASADENDVVQTSGTGRSAHDTTKCKQFKDPLRPEEGSLKKVKPDEKASKLSNGTLARM 1523
+ D + +S + D ++ K KD + LKK K DEK +K+SN
Sbjct: 337 LGVDHNNGDTLASSPKALVSEDASRAKNVKDSHETKGSFLKKPKLDEKPTKVSN------ 390
Query: 1524 TVVPPPGKNL----ISEGQVLEVTRRPDADRSKWFKGLPWEERMQAAHEHGTLVLFENLD 1691
GKNL + G+V+EVTRRPDADRS+WFKGLPWEER++ AHE GTLVL +NLD
Sbjct: 391 ------GKNLKASSLIHGEVVEVTRRPDADRSRWFKGLPWEERIKDAHEQGTLVLIQNLD 444
Query: 1692 PAYCSAEIEDIVWSGFREQCTAKVVPRTSISSPHSGQAFVIFKSGDAADTAVRKLDEGCL 1871
P+Y S E+EDIVW F E CTAK++ +T+ S PH GQA+V+FK+ +AA+ VRKL EGCL
Sbjct: 445 PSYTSGEVEDIVWHAFNESCTAKMIQKTANSMPHIGQAYVVFKTKEAAEKVVRKLHEGCL 504
Query: 1872 MLPNGRPLVASWGSAPSLAGKQSNFFGHLVIGKLKNQMQREEQRDAVSTSHCSQPNTIEY 2051
+L NG LV S+ P L+ K+ FFGH I KL++QMQR E + AVSTSHCSQPNTIEY
Sbjct: 505 LLANGSVLVGSF-ETPHLSSKKQTFFGHHCIDKLRHQMQR-EMKGAVSTSHCSQPNTIEY 562
Query: 2052 DMAMDWCLLQTRSDKMWKGLYKQQ 2123
DMAM+WCLLQ RS+ + K L+KQQ
Sbjct: 563 DMAMEWCLLQERSELVGKQLFKQQ 586
>ref|XP_004136436.1| PREDICTED: uncharacterized protein LOC101208860 [Cucumis sativus]
Length = 600
Score = 514 bits (1323), Expect = e-143
Identities = 293/624 (46%), Positives = 386/624 (61%), Gaps = 8/624 (1%)
Frame = +3
Query: 276 EASEIEDCEFRWXXXXXXXXXXXEVQFYESFTYDGNEYSLYDNVYLYKEDETEPYIGKLV 455
E ++E+ EF+W +V FYESFTYDG EY LYD+VYLYKE E EPYIGKL+
Sbjct: 3 ETEQVENVEFQWGKKKGIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGKLL 62
Query: 456 KIWEQPQQKKKVKVLWFFRQIEILNWLEGVPTLENEIFLASGDGLGLANVNSLEAIAGKC 635
KIW+ P + KKVK+LWFFR EILN+L GV T ENE+FLASG+G+GLAN+NSLE +AGKC
Sbjct: 63 KIWQNPDKTKKVKILWFFRSCEILNYLGGVDTRENELFLASGNGIGLANINSLEVLAGKC 122
Query: 636 HVVCTSKDSRNPQPSQEQLKMADYIFYRTFDVDNYTVSDKINDKIAGVEVRFLLNKEGAQ 815
+V+C S DSRNPQPS E LK AD++F RTFDV V ++I DKIAGVEV+ LLNK A
Sbjct: 123 NVLCISNDSRNPQPSDEALKKADFVFCRTFDVGKQEVCNEICDKIAGVEVKLLLNK--AD 180
Query: 816 QSSGGLKFDSAMKDENGNVMASNEVSELCPKLNSPATLVNTMTDDMPGHPMKKK-DEDSN 992
S + D KD +G + + ++ + + S L T D K+ D +
Sbjct: 181 SSKDVKRTDKDGKDASGIAIVNTQLEDPSGRDISNGELTLTTNDSSLEKSTKENVDLKGS 240
Query: 993 I--SANQNPLPGEDLVSGLG-GETLETEVPTIMQEHVSLYQKTLPSPKSDSCKDEVNTGT 1163
I S+N+ + G+G G+T +I++ L K P K DS + N
Sbjct: 241 IEKSSNEEKSSAHAIERGIGMGKT-----SSIIKHENILGDKVPPKLKIDSNEKPGNAKD 295
Query: 1164 VCISKVGHKKLKSSDDLGVLDDRPSKKARFDTSIKLPEEQCISPSLEANKNKNGNSLQNK 1343
V ++KS + ++ RP+KK + D+S++L + K N ++ K
Sbjct: 296 V------EGRVKSLRESAEVEHRPAKKTKLDSSVQL------------SPGKTENDIE-K 336
Query: 1344 QRASADENDVVQTSGTGRSAHDTTKCKQFKDPLRPEEGSLKKVKPDEKASKLSNGTLARM 1523
+ D + +S + D ++ K KD + LKK K DEK +K+SN
Sbjct: 337 LGVDHNNGDTLASSPKALVSEDASRAKNVKDSHETKGSFLKKPKLDEKPTKVSN------ 390
Query: 1524 TVVPPPGKNL----ISEGQVLEVTRRPDADRSKWFKGLPWEERMQAAHEHGTLVLFENLD 1691
GKNL + G+V+EVTRRPDADRS+WFKGLPWEER++ AHE GTLVL +NLD
Sbjct: 391 ------GKNLKASSLIHGEVVEVTRRPDADRSRWFKGLPWEERIKDAHEQGTLVLIQNLD 444
Query: 1692 PAYCSAEIEDIVWSGFREQCTAKVVPRTSISSPHSGQAFVIFKSGDAADTAVRKLDEGCL 1871
P+Y S E+EDIVW F E CTAK++ +T+ S PH GQA+V+FK+ +AA+ VRKL EGCL
Sbjct: 445 PSYTSGEVEDIVWHAFNESCTAKMIQKTANSMPHIGQAYVVFKTKEAAEKVVRKLHEGCL 504
Query: 1872 MLPNGRPLVASWGSAPSLAGKQSNFFGHLVIGKLKNQMQREEQRDAVSTSHCSQPNTIEY 2051
+L NG LV S+ P L+ K+ FFGH I KL++QMQR E + AVSTSHCSQPNTIEY
Sbjct: 505 LLANGSVLVGSF-ETPHLSSKKQTFFGHHCIDKLRHQMQR-EMKGAVSTSHCSQPNTIEY 562
Query: 2052 DMAMDWCLLQTRSDKMWKGLYKQQ 2123
DMAM+WCLLQ RS+ + K L+KQQ
Sbjct: 563 DMAMEWCLLQERSELVGKQLFKQQ 586
>emb|CBI20922.3| unnamed protein product [Vitis vinifera]
Length = 628
Score = 505 bits (1301), Expect = e-140
Identities = 293/637 (45%), Positives = 387/637 (60%), Gaps = 62/637 (9%)
Frame = +3
Query: 402 NVYLYKEDETEPYIGKLVKIWEQPQQKKKVKVLWFFRQIEILNWLEGVPTLENEIFLASG 581
++ L E E EPYIGKL+KIWE P ++KK+KVLWFF EIL WL T++NE+FLASG
Sbjct: 2 SLLLIMEGEPEPYIGKLIKIWEYPDKEKKIKVLWFFHPSEILKWLGDGETIKNELFLASG 61
Query: 582 DGLGLANVNSLEAIAGKCHVVCTSKDSRNPQPSQEQLKMADYIFYRTFDVDNYTVSDKIN 761
+G+GLANVN LEAIAGKC+VVC SKDSRNPQPS E+L+MAD++FYRTFDV N T+ DKI+
Sbjct: 62 EGVGLANVNPLEAIAGKCNVVCISKDSRNPQPSNEELQMADHVFYRTFDVGNCTILDKID 121
Query: 762 DKIAGVEVRFLLNKEGAQQSSGGLKFDSAMKDENGNVMASNEVSELCPKLNSPATLVNTM 941
DKIAGVEV F+ N+ Q S+ K DS K++ G V+AS+E +L P+ NS +
Sbjct: 122 DKIAGVEVEFIFNRRVCQNSNEVPKMDSNRKEDIGRVVASSETLQL-PEQNSSGEDKDLK 180
Query: 942 TDDMPGHPMKKKDEDSNISANQNPLPGEDLVSGLGGE-TLETEVPTIMQEHVSLYQKTLP 1118
D + ++ D +S ++ S LGG+ + ++ ++S+ ++ +
Sbjct: 181 IDGNSKDALANENVDVKVSLVEHK-------SSLGGKHASDADMVLDNMTNISVERENIV 233
Query: 1119 SPKS----DSCKDEVNTGTVCISKVG-HKKLKSSDDLGVLDDRPSKKARFDTSIKLPEEQ 1283
S DS K + G V +++V +K+KS+ D G+LD RPSK+A+
Sbjct: 234 GDGSKLQVDSVKYDDKVGKVLVNQVEVEEKVKSTRDSGLLDSRPSKRAK----------- 282
Query: 1284 CISPSLEANKNKNGNSLQNKQRASADENDVVQTSGTGRSAHDTTKCKQFKDPLRPEEGSL 1463
+S S E ++++N SLQ K + ++ + T + D TK KD E+ +
Sbjct: 283 -VSSSTELSEDRNNRSLQ-KSNIDSHVKEMKASVSTVTTTKDKTKLDLVKDSPSLEKEAS 340
Query: 1464 KKVKPDEKASKLSNGTLARMTVVPPPGKNLISEGQVLEVTRRPDA-------------DR 1604
KK+K EK +KLSNG L + P + EGQ++EVTRRP+A +
Sbjct: 341 KKLKSYEKMTKLSNGNLIKAFARRSPSVDTKIEGQIMEVTRRPNAVSCIQSSSGPTSLED 400
Query: 1605 SKWFK-------------------------------------------GLPWEERMQAAH 1655
+W K GLPWEERMQ AH
Sbjct: 401 PRWVKAMEAFVAPGPTRLDDCRDPPQSLEGNKAHIGVALPSDRSRWFRGLPWEERMQTAH 460
Query: 1656 EHGTLVLFENLDPAYCSAEIEDIVWSGFREQCTAKVVPRTSISSPHSGQAFVIFKSGDAA 1835
+ GTLVL +NLDPAY SAE+EDIVW GF+E CTA+++ T+ISSPHSGQAFVIFK+ DAA
Sbjct: 461 QQGTLVLLQNLDPAYTSAEVEDIVWHGFKESCTARMIQHTAISSPHSGQAFVIFKTRDAA 520
Query: 1836 DTAVRKLDEGCLMLPNGRPLVASWGSAPSLAGKQSNFFGHLVIGKLKNQMQREEQRDAVS 2015
+A+RKL+EGCL+L NGRPLV S+G+ P GKQS F GHL I K+K QMQR E + AVS
Sbjct: 521 VSAIRKLEEGCLLLSNGRPLVGSFGT-PCFPGKQSTFVGHLSIDKVKVQMQR-EMKQAVS 578
Query: 2016 TSHCSQPNTIEYDMAMDWCLLQTRSDKMWKGLYKQQG 2126
TSHCSQPNTIEY+MAMDWCL Q RSD WK LYK+QG
Sbjct: 579 TSHCSQPNTIEYEMAMDWCLQQERSDSCWKKLYKRQG 615
>ref|XP_002516583.1| hypothetical protein RCOM_0803370 [Ricinus communis]
gi|223544403|gb|EEF45924.1| hypothetical protein
RCOM_0803370 [Ricinus communis]
Length = 730
Score = 484 bits (1247), Expect = e-134
Identities = 288/715 (40%), Positives = 403/715 (56%), Gaps = 98/715 (13%)
Frame = +3
Query: 276 EASEIEDCEFRWXXXXXXXXXXXEVQFYESFTYDGNEYSLYDNVYLYKEDETEPYIGKLV 455
+A E E+ EF+W +VQFYESFT+DG EY LYD+VY+Y + ETEPYIGK++
Sbjct: 3 QAKEDENIEFKWGNKRGVGGRKKDVQFYESFTFDGVEYMLYDSVYMYADIETEPYIGKII 62
Query: 456 KIWEQPQQKKKVKVLWFFRQIEILNWLEGVPTLENEIFLASGDGLGLANVNSLEAIAGKC 635
KIWE + K+VK+LWFFR EI N+LE T +NE+FLASGDG+GLANVN LEAIAGKC
Sbjct: 63 KIWENSDKTKRVKILWFFRPCEISNYLEANETSKNELFLASGDGVGLANVNPLEAIAGKC 122
Query: 636 HVVCTSKDSRNPQPSQEQLKMADYIFYRTFDVDNYTVSDKINDKIAGVEVRFLLNKEGAQ 815
+V+C SKDSRNPQPS E+L+MAD+IF+R FDV + + DKI+DKIA V+V+FLLNK +Q
Sbjct: 123 NVICISKDSRNPQPSNEELQMADFIFFRIFDVGRHVILDKIDDKIAQVDVKFLLNKVNSQ 182
Query: 816 QSSGGLKFDSAMKDENGNVMASNEVSELCPKLNSPATLVNTMTDDMPGHPMKKKDEDSNI 995
+S + DS K+ +GN + + + + L ++N+ +N TDD P+ K+D DS +
Sbjct: 183 KSHVVPERDSIKKEISGNAIVAADGTTLSSEMNAMVERINLKTDDSSIDPLSKEDADSIV 242
Query: 996 -------SANQNP----------LPGEDLVSG--LGGETL-------ETEVPTI-MQEHV 1094
S Q P + D G GG+T+ +E+ T+ +++
Sbjct: 243 LLPNQRSSVGQKPASFSSDELDEIAKIDHAQGDFSGGKTISHSKVKDNSELITLDVKQKS 302
Query: 1095 SLYQK-------------TLPSPKSDSCKDEVNTGTVCISKVGHKKLKSSDDLGVLDDRP 1235
SL +K T+ P D+ D++ + T K L + +
Sbjct: 303 SLGEKPTSNIVGKLAGEATISDPHKDTASDKIRSRTEDEEIADPKPLLVRQRSSLGEKHA 362
Query: 1236 SKKA-RFDTSIK---LPEEQCISPSL-EANKNKNGNSLQNKQRASADENDVV-------- 1376
SK+ R D + K + ++ IS S+ + + + L AS + +
Sbjct: 363 SKELDRIDKNKKQESMSNDKIISRSIGDPIRPSKIDKLGGSSEASGGNKEKIVHKLIPDS 422
Query: 1377 -QTSGTGRSAHDTTKCKQFKDPLRPEEGSLKKVKPDEK----------ASKLSNGTLARM 1523
+ G H K K +D R +LK K D + + G +++
Sbjct: 423 KRCEGKASEVHAEVKVKALEDSCRFANRALKNDKLDGSFKHCDAKAVDRTATTTGEISKR 482
Query: 1524 TVVPPPGKNLI----------------------------------SEGQVLEVTRRPDAD 1601
+V P + I ++ ++LEVTRRP+AD
Sbjct: 483 KLVKDPNETEILSFKKRKLDEKLTKFANGKLPRESPREVSNDVSNTDSKILEVTRRPEAD 542
Query: 1602 RSKWFKGLPWEERMQAAHEHGTLVLFENLDPAYCSAEIEDIVWSGFREQCTAKVVPRTSI 1781
+ KWFKGLPWEER++AAH G +VL +NLDP++ E+EDIVW ++ CTAK++P T+
Sbjct: 543 KIKWFKGLPWEERIKAAHAEGRIVLLQNLDPSFTGLEVEDIVWHALKQSCTAKMIPCTAF 602
Query: 1782 SSPHSGQAFVIFKSGDAADTAVRKLDEGCLMLPNGRPLVASWGSAPSLAGKQSNFFGHLV 1961
SSPHSGQAF IFK+ +AA+TAV KLDEGCLM GRPLV S S GK S +FGH+
Sbjct: 603 SSPHSGQAFAIFKTREAAETAVTKLDEGCLMTSYGRPLVGSIAKL-SFPGKPSTYFGHMS 661
Query: 1962 IGKLKNQMQREEQRDAVSTSHCSQPNTIEYDMAMDWCLLQTRSDKMWKGLYKQQG 2126
I K ++QMQR R+AVSTSHCSQPNTIEYDMA++WC LQ ++++ W+ LYKQ G
Sbjct: 662 IDKTRHQMQR-VMREAVSTSHCSQPNTIEYDMALEWCFLQEQTNQEWELLYKQHG 715
>ref|XP_002324251.1| predicted protein [Populus trichocarpa] gi|222865685|gb|EEF02816.1|
predicted protein [Populus trichocarpa]
Length = 542
Score = 474 bits (1219), Expect = e-131
Identities = 276/618 (44%), Positives = 355/618 (57%), Gaps = 2/618 (0%)
Frame = +3
Query: 276 EASEIEDCEFRWXXXXXXXXXXXEVQFYESFTYDGNEYSLYDNVYLYKEDETEPYIGKLV 455
EA +++ EF+W +VQFYESF YDG +Y+LYD+VY+YKE E EPYIGKL+
Sbjct: 3 EAEKVDCIEFKWCKKRGVGGKKKDVQFYESFFYDGVDYTLYDSVYMYKEGEPEPYIGKLI 62
Query: 456 KIWEQPQQKKKVKVLWFFRQIEILNWLEGVPTLENEIFLASGDGLGLANVNSLEAIAGKC 635
KIWE + KKVKVLWFFR EI N+L TL+NE+FLASG+G+G ANVN LEAIAGKC
Sbjct: 63 KIWENADKTKKVKVLWFFRPREISNYLGDEKTLKNELFLASGEGVGNANVNPLEAIAGKC 122
Query: 636 HVVCTSKDSRNPQPSQEQLKMADYIFYRTFDVDNYTVSDKINDKIAGVEVRFLLNKEGAQ 815
+VVC+SKDSRNP PS E+L+ AD++FYR FDV N + D I++KIAG+EV+FLLN+ G Q
Sbjct: 123 NVVCSSKDSRNPLPSDEELQEADFVFYRAFDVGNCRILDMIDEKIAGIEVKFLLNRVGNQ 182
Query: 816 QSSGGLKFDSAMKDENGNVMASNEVSELCPKLNSPATLVNTMTDDMPGHPMKKKDEDSNI 995
SSG K DS K+ +GN + +D+ I
Sbjct: 183 NSSGVPKLDSNKKEVSGNAGVT---------------------------------DDTRI 209
Query: 996 SANQNPLPGEDLVSGLGGETLETEVPTIMQEHVSLYQKTLPSPKSDSCKDEVNTGTVCIS 1175
A + GE S G + E + N V +
Sbjct: 210 LAKKESYLGEKAASSSGVQFDEVA--------------------------KTNERQVLVE 243
Query: 1176 KVGHKKLKSSDDLGVLDDRPSKKARFDTSIKLPEEQCISPSLEANKNKNGNSLQNKQRAS 1355
+ +LK + G LDDR KKA+ D S K + N +++ Q+
Sbjct: 244 E----ELKVAKASGDLDDRSCKKAKLDDSAKASHD---------------NKVKSTQKLR 284
Query: 1356 ADENDVVQ--TSGTGRSAHDTTKCKQFKDPLRPEEGSLKKVKPDEKASKLSNGTLARMTV 1529
D ND + +A D ++ KDP +K KPDEK L+NG ++
Sbjct: 285 HDSNDSSSKAVAQITPAAEDKSRPNLTKDPQETNNALSEKPKPDEK---LANGKFPEASL 341
Query: 1530 VPPPGKNLISEGQVLEVTRRPDADRSKWFKGLPWEERMQAAHEHGTLVLFENLDPAYCSA 1709
P + + ++ E TRRP PWEE MQ AHE GTLVL +NLDP+Y SA
Sbjct: 342 RQPSEEGSKTSYKIQEPTRRP---------ATPWEETMQTAHEQGTLVLLQNLDPSYTSA 392
Query: 1710 EIEDIVWSGFREQCTAKVVPRTSISSPHSGQAFVIFKSGDAADTAVRKLDEGCLMLPNGR 1889
E+ED++W F++ CTAK++ RT+ SSPHSGQAFVIF+ + A+ AV KLDE CLML NGR
Sbjct: 393 EVEDLIWQAFKQSCTAKMIQRTARSSPHSGQAFVIFQKREVAEMAVAKLDEVCLMLSNGR 452
Query: 1890 PLVASWGSAPSLAGKQSNFFGHLVIGKLKNQMQREEQRDAVSTSHCSQPNTIEYDMAMDW 2069
PLV S +AP GKQS FFGHL I KL+ MQR E ++AVSTSHCSQPNT+EYDMAM+W
Sbjct: 453 PLVGSI-AAPCFPGKQSTFFGHLTINKLRIHMQR-EMKEAVSTSHCSQPNTLEYDMAMEW 510
Query: 2070 CLLQTRSDKMWKGLYKQQ 2123
CLLQ RSD + L +QQ
Sbjct: 511 CLLQDRSDLALRKLRQQQ 528