BLASTX nr result
ID: Cimicifuga21_contig00007338
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cimicifuga21_contig00007338
(1848 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI23362.3| unnamed protein product [Vitis vinifera] 719 0.0
ref|XP_002272174.1| PREDICTED: protein TRANSPARENT TESTA 12-like... 719 0.0
ref|XP_002527924.1| multidrug resistance pump, putative [Ricinus... 712 0.0
ref|XP_002333719.1| predicted protein [Populus trichocarpa] gi|2... 696 0.0
ref|XP_002329229.1| predicted protein [Populus trichocarpa] gi|2... 693 0.0
>emb|CBI23362.3| unnamed protein product [Vitis vinifera]
Length = 1152
Score = 719 bits (1855), Expect = 0.0
Identities = 364/495 (73%), Positives = 414/495 (83%), Gaps = 1/495 (0%)
Frame = -2
Query: 1820 QQPLLLYRKEDEISDQFIKHSGSSFGSSLIAD-GDIQPINRVNDFFAEFLVESKKLWFLA 1644
+QPLL R++DE + + + +S ++ AD DI PIN V DF+ EF+VESKKLW+LA
Sbjct: 648 KQPLLSARQDDETPN---RTAANSLLATFNADESDIGPINGVRDFYKEFIVESKKLWYLA 704
Query: 1643 GPAIFTSICQYSLGAITQVFAGQVGTIELAAVSVENSVIAGFSFGVMLGMGSALETLCGQ 1464
GPAIFTS+CQYSLGAITQVFAG VGT+ELAAVSVENSVIAGFSFGVMLGMGSALETLCGQ
Sbjct: 705 GPAIFTSLCQYSLGAITQVFAGHVGTLELAAVSVENSVIAGFSFGVMLGMGSALETLCGQ 764
Query: 1463 AFGAGQLDMLGIYMQRSWIILGVTSLLLAILYIFAAPLLKLIGQEESIAAAAGKFAIWMI 1284
AFGAGQLDMLG+YMQRSW+IL T++LL+ LYIF+A LLKLIGQ E+I+ AG FA+WM+
Sbjct: 765 AFGAGQLDMLGVYMQRSWVILTSTAVLLSFLYIFSARLLKLIGQTEAISKEAGMFAVWML 824
Query: 1283 PQLFAYAMNFPIAKFLQAQSKMMVMAVIAFAALVLHTFFSWLLMLKLGWGLVGAAVVLNA 1104
PQLFAYA+NFP+AKFLQAQSK+MVMAVIA LVLHT FSWLLMLKL WGLVGAAVVLNA
Sbjct: 825 PQLFAYAVNFPLAKFLQAQSKIMVMAVIAAVVLVLHTVFSWLLMLKLQWGLVGAAVVLNA 884
Query: 1103 SWWFINVAQLLYIFSGTCGRAWSGFSMKAFENLWSFIKLSIASAVMLCLEIWYFMALILF 924
SW I+VAQLLYIFSGTCGRAW+GFS KAF++LW F++LS+ASAVMLCLE+WYFMALILF
Sbjct: 885 SWVLIDVAQLLYIFSGTCGRAWTGFSWKAFQSLWGFVRLSLASAVMLCLEVWYFMALILF 944
Query: 923 AGYLKNPEISVDALSICMNILGWAVMVAIGFNAATSVRVSNELGAGHPRTAKFXXXXXXX 744
AGYLKN EISVDALSICMNILGW VMVA+G NAA SVRVSNELGA HPRTAKF
Sbjct: 945 AGYLKNAEISVDALSICMNILGWTVMVALGCNAAISVRVSNELGATHPRTAKFAVVVVVI 1004
Query: 743 XXXXXXXXXXXXXXIMRKQYPYLFTSSPDVRMLVYQLTPLLSFSIVINNVQPVLSGVAIG 564
I RKQYP LF+ S +V LV +LTPLL+F IVINNVQPVLSGVAIG
Sbjct: 1005 TSFLIGLVLSAILIIFRKQYPALFSDSEEVEELVDELTPLLAFCIVINNVQPVLSGVAIG 1064
Query: 563 AGWQALVAYVNIGCYYLFGIPLGLILGYKLDFGVRGIWLGMIAGTVLQTCILFFMTYRTN 384
AGWQA VA+VNI CYYLFG+PLGL+LGYK+ GV+GIW GM++GTV+QTC+LF M YRTN
Sbjct: 1065 AGWQAFVAWVNIACYYLFGVPLGLVLGYKVGLGVKGIWCGMLSGTVVQTCVLFGMVYRTN 1124
Query: 383 WNKEASDAEVRIKQW 339
WNKEAS A RIK+W
Sbjct: 1125 WNKEASIAGDRIKKW 1139
Score = 667 bits (1721), Expect = 0.0
Identities = 328/505 (64%), Positives = 395/505 (78%), Gaps = 2/505 (0%)
Frame = -2
Query: 1847 PIMEDTHQQQQPLLLYRKEDEISDQFIKHSGSSFGSSLIA--DGDIQPINRVNDFFAEFL 1674
P++ TH Q+Q Q + + + F S L A D D+ PIN V DFF EF
Sbjct: 36 PLLSTTHDQEQT------------QNPRPAKTGFFSFLAANYDDDMDPINGVGDFFREFR 83
Query: 1673 VESKKLWFLAGPAIFTSICQYSLGAITQVFAGQVGTIELAAVSVENSVIAGFSFGVMLGM 1494
VESKKLW +AGPAIFT +C+YSLGAITQVF G +G ++LAA +VENSVI+ FS G MLGM
Sbjct: 84 VESKKLWHIAGPAIFTYLCRYSLGAITQVFVGHIGALQLAAFAVENSVISMFSLGTMLGM 143
Query: 1493 GSALETLCGQAFGAGQLDMLGIYMQRSWIILGVTSLLLAILYIFAAPLLKLIGQEESIAA 1314
GSALETLCGQAFGAGQLDMLG+YMQRSWIIL T+L L+++YIFA +LKLIG+ E I+
Sbjct: 144 GSALETLCGQAFGAGQLDMLGVYMQRSWIILVTTALPLSLIYIFAEQILKLIGETEEISK 203
Query: 1313 AAGKFAIWMIPQLFAYAMNFPIAKFLQAQSKMMVMAVIAFAALVLHTFFSWLLMLKLGWG 1134
AAG FA+WM+PQLF+YA++FPI+KFLQ+Q KM+V+++ A LVLH FFSWLL++KLGWG
Sbjct: 204 AAGVFALWMLPQLFSYALSFPISKFLQSQRKMLVLSLTAGVTLVLHAFFSWLLIMKLGWG 263
Query: 1133 LVGAAVVLNASWWFINVAQLLYIFSGTCGRAWSGFSMKAFENLWSFIKLSIASAVMLCLE 954
LVGAAVVLN S W A YI SG+CG+AWSGFS KAF+NLW+F+KLS+ASAVMLCLE
Sbjct: 264 LVGAAVVLNVSSWLPVAANFSYILSGSCGQAWSGFSSKAFQNLWAFVKLSLASAVMLCLE 323
Query: 953 IWYFMALILFAGYLKNPEISVDALSICMNILGWAVMVAIGFNAATSVRVSNELGAGHPRT 774
+WYFM LILFAGYLKN E+SVDALSICM ILGWAVM++IGFNAA SVRVSNELGA HPRT
Sbjct: 324 VWYFMVLILFAGYLKNAEVSVDALSICMTILGWAVMLSIGFNAAVSVRVSNELGASHPRT 383
Query: 773 AKFXXXXXXXXXXXXXXXXXXXXXIMRKQYPYLFTSSPDVRMLVYQLTPLLSFSIVINNV 594
AKF R+QYP LF+S+ +++ LVY LTPLL+ IVINN+
Sbjct: 384 AKFSVAVAAITSFLISVVLSLILIAARRQYPDLFSSNAEIKKLVYSLTPLLAVCIVINNI 443
Query: 593 QPVLSGVAIGAGWQALVAYVNIGCYYLFGIPLGLILGYKLDFGVRGIWLGMIAGTVLQTC 414
QPVLSGVA+GAGWQA +AYVNIGCYY+ G+P+GL+LG+KLD GV+GIW GM++GTV+QTC
Sbjct: 444 QPVLSGVAVGAGWQAFIAYVNIGCYYVIGVPMGLLLGFKLDLGVKGIWCGMLSGTVIQTC 503
Query: 413 ILFFMTYRTNWNKEASDAEVRIKQW 339
ILF M YRTNWN EAS AE RI++W
Sbjct: 504 ILFGMIYRTNWNTEASMAEERIRKW 528
>ref|XP_002272174.1| PREDICTED: protein TRANSPARENT TESTA 12-like [Vitis vinifera]
Length = 544
Score = 719 bits (1855), Expect = 0.0
Identities = 364/495 (73%), Positives = 414/495 (83%), Gaps = 1/495 (0%)
Frame = -2
Query: 1820 QQPLLLYRKEDEISDQFIKHSGSSFGSSLIAD-GDIQPINRVNDFFAEFLVESKKLWFLA 1644
+QPLL R++DE + + + +S ++ AD DI PIN V DF+ EF+VESKKLW+LA
Sbjct: 40 KQPLLSARQDDETPN---RTAANSLLATFNADESDIGPINGVRDFYKEFIVESKKLWYLA 96
Query: 1643 GPAIFTSICQYSLGAITQVFAGQVGTIELAAVSVENSVIAGFSFGVMLGMGSALETLCGQ 1464
GPAIFTS+CQYSLGAITQVFAG VGT+ELAAVSVENSVIAGFSFGVMLGMGSALETLCGQ
Sbjct: 97 GPAIFTSLCQYSLGAITQVFAGHVGTLELAAVSVENSVIAGFSFGVMLGMGSALETLCGQ 156
Query: 1463 AFGAGQLDMLGIYMQRSWIILGVTSLLLAILYIFAAPLLKLIGQEESIAAAAGKFAIWMI 1284
AFGAGQLDMLG+YMQRSW+IL T++LL+ LYIF+A LLKLIGQ E+I+ AG FA+WM+
Sbjct: 157 AFGAGQLDMLGVYMQRSWVILTSTAVLLSFLYIFSARLLKLIGQTEAISKEAGMFAVWML 216
Query: 1283 PQLFAYAMNFPIAKFLQAQSKMMVMAVIAFAALVLHTFFSWLLMLKLGWGLVGAAVVLNA 1104
PQLFAYA+NFP+AKFLQAQSK+MVMAVIA LVLHT FSWLLMLKL WGLVGAAVVLNA
Sbjct: 217 PQLFAYAVNFPLAKFLQAQSKIMVMAVIAAVVLVLHTVFSWLLMLKLQWGLVGAAVVLNA 276
Query: 1103 SWWFINVAQLLYIFSGTCGRAWSGFSMKAFENLWSFIKLSIASAVMLCLEIWYFMALILF 924
SW I+VAQLLYIFSGTCGRAW+GFS KAF++LW F++LS+ASAVMLCLE+WYFMALILF
Sbjct: 277 SWVLIDVAQLLYIFSGTCGRAWTGFSWKAFQSLWGFVRLSLASAVMLCLEVWYFMALILF 336
Query: 923 AGYLKNPEISVDALSICMNILGWAVMVAIGFNAATSVRVSNELGAGHPRTAKFXXXXXXX 744
AGYLKN EISVDALSICMNILGW VMVA+G NAA SVRVSNELGA HPRTAKF
Sbjct: 337 AGYLKNAEISVDALSICMNILGWTVMVALGCNAAISVRVSNELGATHPRTAKFAVVVVVI 396
Query: 743 XXXXXXXXXXXXXXIMRKQYPYLFTSSPDVRMLVYQLTPLLSFSIVINNVQPVLSGVAIG 564
I RKQYP LF+ S +V LV +LTPLL+F IVINNVQPVLSGVAIG
Sbjct: 397 TSFLIGLVLSAILIIFRKQYPALFSDSEEVEELVDELTPLLAFCIVINNVQPVLSGVAIG 456
Query: 563 AGWQALVAYVNIGCYYLFGIPLGLILGYKLDFGVRGIWLGMIAGTVLQTCILFFMTYRTN 384
AGWQA VA+VNI CYYLFG+PLGL+LGYK+ GV+GIW GM++GTV+QTC+LF M YRTN
Sbjct: 457 AGWQAFVAWVNIACYYLFGVPLGLVLGYKVGLGVKGIWCGMLSGTVVQTCVLFGMVYRTN 516
Query: 383 WNKEASDAEVRIKQW 339
WNKEAS A RIK+W
Sbjct: 517 WNKEASIAGDRIKKW 531
>ref|XP_002527924.1| multidrug resistance pump, putative [Ricinus communis]
gi|223532699|gb|EEF34481.1| multidrug resistance pump,
putative [Ricinus communis]
Length = 531
Score = 712 bits (1838), Expect = 0.0
Identities = 350/487 (71%), Positives = 407/487 (83%), Gaps = 1/487 (0%)
Frame = -2
Query: 1760 SGSSFGSSLIADGD-IQPINRVNDFFAEFLVESKKLWFLAGPAIFTSICQYSLGAITQVF 1584
+ +SF S+ + D D I PI +FF EF ESKKLW+LAGPAIFTSICQYSLGA+TQVF
Sbjct: 40 NNNSFSSAFVPDSDDIPPITGFRNFFREFYRESKKLWYLAGPAIFTSICQYSLGAVTQVF 99
Query: 1583 AGQVGTIELAAVSVENSVIAGFSFGVMLGMGSALETLCGQAFGAGQLDMLGIYMQRSWII 1404
+GQVGT++LAAVSVENSVIAGFSFG+MLGMGSALETLCGQA+GA QLDMLGIY+QRSW+I
Sbjct: 100 SGQVGTLDLAAVSVENSVIAGFSFGIMLGMGSALETLCGQAYGAKQLDMLGIYLQRSWVI 159
Query: 1403 LGVTSLLLAILYIFAAPLLKLIGQEESIAAAAGKFAIWMIPQLFAYAMNFPIAKFLQAQS 1224
LG T+ L +LY+FAA +LK IGQ +I+ AAG FAIWMIPQLFAYAMNFP+AKFLQAQS
Sbjct: 160 LGTTASFLCLLYVFAAQILKSIGQTPAISKAAGVFAIWMIPQLFAYAMNFPMAKFLQAQS 219
Query: 1223 KMMVMAVIAFAALVLHTFFSWLLMLKLGWGLVGAAVVLNASWWFINVAQLLYIFSGTCGR 1044
K+MVMA+IA AALVLH FSWLLMLKLGWGLVGAAVVLNASWWFI++AQ LYI SGTCGR
Sbjct: 220 KIMVMAMIAAAALVLHAVFSWLLMLKLGWGLVGAAVVLNASWWFIDLAQFLYIISGTCGR 279
Query: 1043 AWSGFSMKAFENLWSFIKLSIASAVMLCLEIWYFMALILFAGYLKNPEISVDALSICMNI 864
AW+GFS KAF+NLWSF++LS+ASAVMLCLE+WYFMALILFAGYLKN E+SVDALSICMNI
Sbjct: 280 AWNGFSWKAFQNLWSFVRLSLASAVMLCLEVWYFMALILFAGYLKNAEVSVDALSICMNI 339
Query: 863 LGWAVMVAIGFNAATSVRVSNELGAGHPRTAKFXXXXXXXXXXXXXXXXXXXXXIMRKQY 684
LGW VM+AIG NAA SVRVSNELGAGHPRTAKF + R Y
Sbjct: 340 LGWTVMIAIGMNAAISVRVSNELGAGHPRTAKFSLVVAVISSFLIGVVLSLILFLSRNSY 399
Query: 683 PYLFTSSPDVRMLVYQLTPLLSFSIVINNVQPVLSGVAIGAGWQALVAYVNIGCYYLFGI 504
P LF++ +V+ LV +LTP+L+ I+INNVQPVLSGVAIGAGWQA+VAYVNI CYY+FGI
Sbjct: 400 PSLFSNDSEVKELVNELTPVLAACIIINNVQPVLSGVAIGAGWQAIVAYVNIACYYVFGI 459
Query: 503 PLGLILGYKLDFGVRGIWLGMIAGTVLQTCILFFMTYRTNWNKEASDAEVRIKQWKVEAE 324
P+GLILGYK+ +GVRGIW GM++GTV+QTC LF+M Y+TNWNKEAS AE RI++W +
Sbjct: 460 PMGLILGYKVGWGVRGIWYGMMSGTVVQTCALFWMIYKTNWNKEASIAEDRIRKWGGHTD 519
Query: 323 DSVSDFE 303
V++ E
Sbjct: 520 SGVNNKE 526
>ref|XP_002333719.1| predicted protein [Populus trichocarpa] gi|222837995|gb|EEE76360.1|
predicted protein [Populus trichocarpa]
Length = 512
Score = 696 bits (1796), Expect = 0.0
Identities = 346/495 (69%), Positives = 398/495 (80%), Gaps = 1/495 (0%)
Frame = -2
Query: 1820 QQPLLLYRKEDEISDQFIKHSGSSFGSSLIADGDIQP-INRVNDFFAEFLVESKKLWFLA 1644
+QPLL + + + DQ ++ + SS SS D D P IN V DF EF ESKKLW+LA
Sbjct: 5 RQPLLSPQNDHQNRDQHLRSTNSSIASSFTPDPDDIPLINSVRDFSIEFCRESKKLWYLA 64
Query: 1643 GPAIFTSICQYSLGAITQVFAGQVGTIELAAVSVENSVIAGFSFGVMLGMGSALETLCGQ 1464
GPAIFT++CQYSLGAITQ+ AG VGT++LAAVSVENSVIAGFSFG+MLGMGSALETLCGQ
Sbjct: 65 GPAIFTTLCQYSLGAITQLLAGHVGTLDLAAVSVENSVIAGFSFGIMLGMGSALETLCGQ 124
Query: 1463 AFGAGQLDMLGIYMQRSWIILGVTSLLLAILYIFAAPLLKLIGQEESIAAAAGKFAIWMI 1284
A+GAGQLDMLG+YMQRSW+IL T+++L +LYIFA P LKLIGQ I+ AAG F++WMI
Sbjct: 125 AYGAGQLDMLGLYMQRSWVILNATAVILTLLYIFAGPFLKLIGQTAEISQAAGMFSVWMI 184
Query: 1283 PQLFAYAMNFPIAKFLQAQSKMMVMAVIAFAALVLHTFFSWLLMLKLGWGLVGAAVVLNA 1104
PQLFAYAMNFPIAKFLQ+QSKMMVMA IA ALV H FSWLLMLKLGWGLVGAAVVLNA
Sbjct: 185 PQLFAYAMNFPIAKFLQSQSKMMVMAAIAAVALVFHAVFSWLLMLKLGWGLVGAAVVLNA 244
Query: 1103 SWWFINVAQLLYIFSGTCGRAWSGFSMKAFENLWSFIKLSIASAVMLCLEIWYFMALILF 924
SWWFI +AQLLYIFSGTCG AW+G S KAF+NLW F++LS+ASAVMLCLE+WYFMALILF
Sbjct: 245 SWWFIVIAQLLYIFSGTCGEAWTGLSWKAFQNLWGFVRLSLASAVMLCLEVWYFMALILF 304
Query: 923 AGYLKNPEISVDALSICMNILGWAVMVAIGFNAATSVRVSNELGAGHPRTAKFXXXXXXX 744
AGYLKN E++VDALSI NI+GWAVM+AIG NAA SVRVSNELGA HPRTAKF
Sbjct: 305 AGYLKNAELAVDALSISTNIVGWAVMIAIGINAAISVRVSNELGAAHPRTAKFSLVVATL 364
Query: 743 XXXXXXXXXXXXXXIMRKQYPYLFTSSPDVRMLVYQLTPLLSFSIVINNVQPVLSGVAIG 564
+ R YP LFT+ V+ LV +LTPLL+ I+INNVQPVLSGVAIG
Sbjct: 365 ASLMIGLVIALILVLARNLYPDLFTNDAGVKELVKELTPLLAVCIIINNVQPVLSGVAIG 424
Query: 563 AGWQALVAYVNIGCYYLFGIPLGLILGYKLDFGVRGIWLGMIAGTVLQTCILFFMTYRTN 384
AGWQA VAYVNIGCYY+FGIPLGLILGY L GV+GIW+GM+ GT +QT +LF+M +TN
Sbjct: 425 AGWQAAVAYVNIGCYYIFGIPLGLILGYWLQMGVQGIWIGMLTGTAVQTAVLFWMIGKTN 484
Query: 383 WNKEASDAEVRIKQW 339
WN EAS AE RI++W
Sbjct: 485 WNTEASAAEERIRKW 499
>ref|XP_002329229.1| predicted protein [Populus trichocarpa] gi|222871010|gb|EEF08141.1|
predicted protein [Populus trichocarpa]
Length = 470
Score = 693 bits (1788), Expect = 0.0
Identities = 342/461 (74%), Positives = 389/461 (84%)
Frame = -2
Query: 1721 DIQPINRVNDFFAEFLVESKKLWFLAGPAIFTSICQYSLGAITQVFAGQVGTIELAAVSV 1542
DIQPIN V DFF EF ES KLWFLA PAIFT++CQYSLGAITQVFAG VGT+ LAAVS+
Sbjct: 2 DIQPINSVRDFFREFCRESVKLWFLAAPAIFTTVCQYSLGAITQVFAGHVGTLALAAVSI 61
Query: 1541 ENSVIAGFSFGVMLGMGSALETLCGQAFGAGQLDMLGIYMQRSWIILGVTSLLLAILYIF 1362
ENSVIAGFSFG+MLGMGSALETLCGQAFGAGQLDMLGIYMQRSW+IL T+LLL+++YIF
Sbjct: 62 ENSVIAGFSFGLMLGMGSALETLCGQAFGAGQLDMLGIYMQRSWLILNTTALLLSLVYIF 121
Query: 1361 AAPLLKLIGQEESIAAAAGKFAIWMIPQLFAYAMNFPIAKFLQAQSKMMVMAVIAFAALV 1182
+A LLKLIGQ SI+ AAG F+IWM+PQLFAYA NFP+AKFLQ+QSK+MVMAVI+ L+
Sbjct: 122 SAQLLKLIGQTASISKAAGMFSIWMLPQLFAYAFNFPMAKFLQSQSKIMVMAVISVVVLI 181
Query: 1181 LHTFFSWLLMLKLGWGLVGAAVVLNASWWFINVAQLLYIFSGTCGRAWSGFSMKAFENLW 1002
LHT FSWLLM+KL WGLVGAAVVLNASW I+++Q +YI SGTCGRAWSGFS KAF+NLW
Sbjct: 182 LHTVFSWLLMIKLKWGLVGAAVVLNASWVIIDISQFVYIISGTCGRAWSGFSWKAFQNLW 241
Query: 1001 SFIKLSIASAVMLCLEIWYFMALILFAGYLKNPEISVDALSICMNILGWAVMVAIGFNAA 822
SF++LS+ASAVMLCLE+WYFMALILFAGYLKN E++VDALSICMNILGW VMVA+G NAA
Sbjct: 242 SFVRLSLASAVMLCLEVWYFMALILFAGYLKNAEVAVDALSICMNILGWTVMVALGMNAA 301
Query: 821 TSVRVSNELGAGHPRTAKFXXXXXXXXXXXXXXXXXXXXXIMRKQYPYLFTSSPDVRMLV 642
SVRVSNELGA HPRTAKF I RK YP LF+S DV+ LV
Sbjct: 302 ISVRVSNELGAAHPRTAKFALVVAVVSSFIIGLILAAILLIFRKSYPSLFSSDLDVQELV 361
Query: 641 YQLTPLLSFSIVINNVQPVLSGVAIGAGWQALVAYVNIGCYYLFGIPLGLILGYKLDFGV 462
LTPLL+ IVI+NVQPVLSGVAIGAGWQA+VAYVNI CYY+FGIPLGLILG+K+ GV
Sbjct: 362 QDLTPLLALCIVIDNVQPVLSGVAIGAGWQAVVAYVNIACYYIFGIPLGLILGFKVKLGV 421
Query: 461 RGIWLGMIAGTVLQTCILFFMTYRTNWNKEASDAEVRIKQW 339
+GIW GM++GTVLQT ILF + Y+TNWNKEAS AE RI++W
Sbjct: 422 KGIWYGMLSGTVLQTIILFLIIYKTNWNKEASIAEDRIRKW 462