BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00007264
         (1593 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI23872.3| unnamed protein product [Vitis vinifera]              638   e-180
ref|XP_002276384.1| PREDICTED: 3-isopropylmalate dehydrogenase, ...   638   e-180
ref|XP_002298230.1| predicted protein [Populus trichocarpa] gi|2...   637   e-180
ref|XP_002530452.1| 3-isopropylmalate dehydrogenase, putative [R...   636   e-180
sp|P29102.1|LEU3_BRANA RecName: Full=3-isopropylmalate dehydroge...   630   e-178

>emb|CBI23872.3| unnamed protein product [Vitis vinifera]
          Length = 506

 Score =  638 bits (1645), Expect = e-180
 Identities = 327/391 (83%), Positives = 356/391 (91%), Gaps = 3/391 (0%)
 Frame = -2

Query: 1430 FHSKSLSPP---KRSLIRCSAAPTNTKRYNITLLPGDGIGPEVISVAKNVLILAGSQQGI 1260
            F SKSLS P   + + IRCSAA T  KRY+ITLLPGDGIGPEVISVAKNVL LAGS +GI
Sbjct: 117  FQSKSLSKPSSIRLATIRCSAA-TPPKRYSITLLPGDGIGPEVISVAKNVLKLAGSLEGI 175

Query: 1259 EFGFQELPVGGAALDLVGVPLPEETLSIAKQSDAVLLGAIGGYKWDNNEKHLKPETGLLQ 1080
            EFGFQE+PVGG ALDL GVPLPEETL +AKQSDAVLLGAIGGYKWD+NEKHLKPETGLLQ
Sbjct: 176  EFGFQEMPVGGCALDLTGVPLPEETLLVAKQSDAVLLGAIGGYKWDSNEKHLKPETGLLQ 235

Query: 1079 LRAGLGVFANLRPATVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGQPRGFGTNEKG 900
            LR+GL VFANLRPA+VLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFG+PRGF  NE G
Sbjct: 236  LRSGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGKPRGFSKNENG 295

Query: 899  EEIGYNTEVYATYEIDRIARVAFETARKRRGKLCSVDKANVLEASMLWRKRVTMMASHYP 720
            EEIG+NTEVYATYEIDRIAR+AFETA+KRRGKLCSVDKANVLEASMLWRKRVT MAS YP
Sbjct: 296  EEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLWRKRVTAMASEYP 355

Query: 719  DVELTHMYVDNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMLTGSIGMLPSACLGASGP 540
            DVEL+HMYVDNAAMQL+R+PKQFDTIVTNNIFGDILSDEASM+TGSIGMLPSA +G SGP
Sbjct: 356  DVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASVGESGP 415

Query: 539  GLFEPIHGSAPDIAGQDKANPLATVLSVAMLLRYGLQEETAAKLIESAVLETLNKGFRTG 360
            GLFEPIHGSAPDIAGQDKANPLATVLS AMLLRYGL E  AAK IE+AVL+TL++GFRT 
Sbjct: 416  GLFEPIHGSAPDIAGQDKANPLATVLSAAMLLRYGLGEGNAAKRIETAVLDTLDRGFRTR 475

Query: 359  DIFSTGKKLVGCTEIGEEVMKSLDSQKAAAV 267
            DI+  G KLVGC E+GE+V+K+++SQ  AAV
Sbjct: 476  DIYEAGTKLVGCKEMGEQVLKAVESQIRAAV 506


>ref|XP_002276384.1| PREDICTED: 3-isopropylmalate dehydrogenase, chloroplastic [Vitis
            vinifera]
          Length = 405

 Score =  638 bits (1645), Expect = e-180
 Identities = 327/391 (83%), Positives = 356/391 (91%), Gaps = 3/391 (0%)
 Frame = -2

Query: 1430 FHSKSLSPP---KRSLIRCSAAPTNTKRYNITLLPGDGIGPEVISVAKNVLILAGSQQGI 1260
            F SKSLS P   + + IRCSAA T  KRY+ITLLPGDGIGPEVISVAKNVL LAGS +GI
Sbjct: 16   FQSKSLSKPSSIRLATIRCSAA-TPPKRYSITLLPGDGIGPEVISVAKNVLKLAGSLEGI 74

Query: 1259 EFGFQELPVGGAALDLVGVPLPEETLSIAKQSDAVLLGAIGGYKWDNNEKHLKPETGLLQ 1080
            EFGFQE+PVGG ALDL GVPLPEETL +AKQSDAVLLGAIGGYKWD+NEKHLKPETGLLQ
Sbjct: 75   EFGFQEMPVGGCALDLTGVPLPEETLLVAKQSDAVLLGAIGGYKWDSNEKHLKPETGLLQ 134

Query: 1079 LRAGLGVFANLRPATVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGQPRGFGTNEKG 900
            LR+GL VFANLRPA+VLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFG+PRGF  NE G
Sbjct: 135  LRSGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGKPRGFSKNENG 194

Query: 899  EEIGYNTEVYATYEIDRIARVAFETARKRRGKLCSVDKANVLEASMLWRKRVTMMASHYP 720
            EEIG+NTEVYATYEIDRIAR+AFETA+KRRGKLCSVDKANVLEASMLWRKRVT MAS YP
Sbjct: 195  EEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLWRKRVTAMASEYP 254

Query: 719  DVELTHMYVDNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMLTGSIGMLPSACLGASGP 540
            DVEL+HMYVDNAAMQL+R+PKQFDTIVTNNIFGDILSDEASM+TGSIGMLPSA +G SGP
Sbjct: 255  DVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASVGESGP 314

Query: 539  GLFEPIHGSAPDIAGQDKANPLATVLSVAMLLRYGLQEETAAKLIESAVLETLNKGFRTG 360
            GLFEPIHGSAPDIAGQDKANPLATVLS AMLLRYGL E  AAK IE+AVL+TL++GFRT 
Sbjct: 315  GLFEPIHGSAPDIAGQDKANPLATVLSAAMLLRYGLGEGNAAKRIETAVLDTLDRGFRTR 374

Query: 359  DIFSTGKKLVGCTEIGEEVMKSLDSQKAAAV 267
            DI+  G KLVGC E+GE+V+K+++SQ  AAV
Sbjct: 375  DIYEAGTKLVGCKEMGEQVLKAVESQIRAAV 405


>ref|XP_002298230.1| predicted protein [Populus trichocarpa] gi|222845488|gb|EEE83035.1|
            predicted protein [Populus trichocarpa]
          Length = 400

 Score =  637 bits (1644), Expect = e-180
 Identities = 330/404 (81%), Positives = 361/404 (89%), Gaps = 1/404 (0%)
 Frame = -2

Query: 1475 MSTLHPKIKTLNTFPFHSKSLSPPKRSLIRCSAAPTN-TKRYNITLLPGDGIGPEVISVA 1299
            ++ LHP IK +  F     SL P   S IRC+AA T  +KRY+ITLLPGDGIGPEVISVA
Sbjct: 3    INALHP-IKPI--FLLKPASLRP---SRIRCAAATTKQSKRYSITLLPGDGIGPEVISVA 56

Query: 1298 KNVLILAGSQQGIEFGFQELPVGGAALDLVGVPLPEETLSIAKQSDAVLLGAIGGYKWDN 1119
            KNVL LAGS +GIEF FQE+P+GG+ALD+ GVPLP+ETL  AK+SDAVLLGAIGGYKWD 
Sbjct: 57   KNVLNLAGSLEGIEFNFQEMPMGGSALDMAGVPLPDETLLAAKKSDAVLLGAIGGYKWDK 116

Query: 1118 NEKHLKPETGLLQLRAGLGVFANLRPATVLPQLVDASTLKKEVAEGVDLMVVRELTGGIY 939
            NEKHLKPETGLLQLR GL VFANLRPATVLPQLVDASTLKKEVAEGVD+MVVRELTGGIY
Sbjct: 117  NEKHLKPETGLLQLREGLEVFANLRPATVLPQLVDASTLKKEVAEGVDVMVVRELTGGIY 176

Query: 938  FGQPRGFGTNEKGEEIGYNTEVYATYEIDRIARVAFETARKRRGKLCSVDKANVLEASML 759
            FG+PRGFGTNE GEEIG+NTEVYATYE+DRIARVAFETARKR GKLCSVDKANVLEASM 
Sbjct: 177  FGKPRGFGTNEDGEEIGFNTEVYATYEVDRIARVAFETARKRHGKLCSVDKANVLEASMF 236

Query: 758  WRKRVTMMASHYPDVELTHMYVDNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMLTGSI 579
            WRKRV  +A+ YPDVEL+HMYVDNAAMQL+R+PKQFDTIVTNNIFGDILSDEASM+TGSI
Sbjct: 237  WRKRVISIAAEYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITGSI 296

Query: 578  GMLPSACLGASGPGLFEPIHGSAPDIAGQDKANPLATVLSVAMLLRYGLQEETAAKLIES 399
            GMLPSA LG+SGPGLFEPIHGSAPDIA QDKANPLATVLS AMLL+YGL EE AAK IE+
Sbjct: 297  GMLPSASLGSSGPGLFEPIHGSAPDIANQDKANPLATVLSAAMLLKYGLGEENAAKRIEN 356

Query: 398  AVLETLNKGFRTGDIFSTGKKLVGCTEIGEEVMKSLDSQKAAAV 267
            AVL+TLNKGFRTGDI+S G KLVGC E+GEEV+KS+DSQ AAAV
Sbjct: 357  AVLDTLNKGFRTGDIYSAGNKLVGCKEMGEEVLKSVDSQVAAAV 400


>ref|XP_002530452.1| 3-isopropylmalate dehydrogenase, putative [Ricinus communis]
            gi|223529997|gb|EEF31922.1| 3-isopropylmalate
            dehydrogenase, putative [Ricinus communis]
          Length = 406

 Score =  636 bits (1640), Expect = e-180
 Identities = 324/392 (82%), Positives = 355/392 (90%), Gaps = 4/392 (1%)
 Frame = -2

Query: 1430 FHSKSLSP----PKRSLIRCSAAPTNTKRYNITLLPGDGIGPEVISVAKNVLILAGSQQG 1263
            FHSK  S     P R  I+C AA T +KRY+ITLLPGDGIGPEVIS+AKNVL LAG+ +G
Sbjct: 16   FHSKLTSKHCLKPGRFTIKC-AAVTPSKRYSITLLPGDGIGPEVISIAKNVLKLAGNLEG 74

Query: 1262 IEFGFQELPVGGAALDLVGVPLPEETLSIAKQSDAVLLGAIGGYKWDNNEKHLKPETGLL 1083
            IEF FQE+P+GG+ALDLVGVPLP+ETLS+AKQSDAVLLGAIGGYKWDNNEKHLKPETGLL
Sbjct: 75   IEFSFQEMPMGGSALDLVGVPLPDETLSVAKQSDAVLLGAIGGYKWDNNEKHLKPETGLL 134

Query: 1082 QLRAGLGVFANLRPATVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGQPRGFGTNEK 903
            QLR GL VFANLRPATVLPQLVDASTLKKEVAEGVD+MVVRELTGGIYFG+PRGFGTNE 
Sbjct: 135  QLREGLEVFANLRPATVLPQLVDASTLKKEVAEGVDVMVVRELTGGIYFGKPRGFGTNES 194

Query: 902  GEEIGYNTEVYATYEIDRIARVAFETARKRRGKLCSVDKANVLEASMLWRKRVTMMASHY 723
            GEEIG+NTEVYA YE+DRIARVAFETARKR GKLCSVDKANVLEASM WRKRV  +A  Y
Sbjct: 195  GEEIGFNTEVYAGYEVDRIARVAFETARKRSGKLCSVDKANVLEASMFWRKRVIAIAEEY 254

Query: 722  PDVELTHMYVDNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMLTGSIGMLPSACLGASG 543
            PDVEL+HMYVDNAAMQL+R+PKQFDTIVTNNIFGDILSDEASM+TGSIGMLPSA LG+SG
Sbjct: 255  PDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGSSG 314

Query: 542  PGLFEPIHGSAPDIAGQDKANPLATVLSVAMLLRYGLQEETAAKLIESAVLETLNKGFRT 363
            PGLFEPIHGSAPDIAGQDKANPLATVLS AMLL+YGL E+ AAK IE+AVL+TL+ GFRT
Sbjct: 315  PGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEDKAAKRIENAVLDTLDMGFRT 374

Query: 362  GDIFSTGKKLVGCTEIGEEVMKSLDSQKAAAV 267
            GDI+S G KLVGC E+G+EV+KS+DSQ  AAV
Sbjct: 375  GDIYSAGTKLVGCKEMGDEVLKSVDSQVPAAV 406


>sp|P29102.1|LEU3_BRANA RecName: Full=3-isopropylmalate dehydrogenase, chloroplastic;
            Short=3-IPM-DH; Short=IMDH; AltName: Full=Beta-IPM
            dehydrogenase; Flags: Precursor gi|17827|emb|CAA42596.1|
            3-isopropylmalate dehydrogenase [Brassica napus]
          Length = 406

 Score =  630 bits (1624), Expect = e-178
 Identities = 323/407 (79%), Positives = 354/407 (86%), Gaps = 3/407 (0%)
 Frame = -2

Query: 1478 MMSTLHPKIKTLNTFPFHSKSL---SPPKRSLIRCSAAPTNTKRYNITLLPGDGIGPEVI 1308
            M + L   I+ +  FP   ++L   S P    +RC+AA    KRYNITLLPGDGIGPEVI
Sbjct: 1    MAAALQTNIRPVK-FPATLRALTKQSSPAPFRVRCAAASPGKKRYNITLLPGDGIGPEVI 59

Query: 1307 SVAKNVLILAGSQQGIEFGFQELPVGGAALDLVGVPLPEETLSIAKQSDAVLLGAIGGYK 1128
            S+AKNVL  AGS +G+EF FQE+PVGGAALDLVGVPLPEET+S AK+SDAVLLGAIGGYK
Sbjct: 60   SIAKNVLQQAGSLEGLEFSFQEMPVGGAALDLVGVPLPEETVSAAKESDAVLLGAIGGYK 119

Query: 1127 WDNNEKHLKPETGLLQLRAGLGVFANLRPATVLPQLVDASTLKKEVAEGVDLMVVRELTG 948
            WD NEKHLKPETGLLQLRAGL VFANLRPATVLPQLVDASTLK+EVAEGVDLMVVRELTG
Sbjct: 120  WDKNEKHLKPETGLLQLRAGLKVFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTG 179

Query: 947  GIYFGQPRGFGTNEKGEEIGYNTEVYATYEIDRIARVAFETARKRRGKLCSVDKANVLEA 768
            GIYFG PRG  TNE GEE+GYNTEVYA +EIDRIARVAFETARKRRGKLCSVDKANVL+A
Sbjct: 180  GIYFGVPRGIKTNENGEEVGYNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLDA 239

Query: 767  SMLWRKRVTMMASHYPDVELTHMYVDNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMLT 588
            S+LWR+RVT +A+ YPDVEL+HMYVDNAAMQL+RDPKQFDTIVTNNIFGDILSDEASM+T
Sbjct: 240  SILWRRRVTALAAEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMIT 299

Query: 587  GSIGMLPSACLGASGPGLFEPIHGSAPDIAGQDKANPLATVLSVAMLLRYGLQEETAAKL 408
            GSIGMLPSA L  SGPGLFEPIHGSAPDIAGQDKANPLAT+LS AMLL+YGL EE AAK 
Sbjct: 300  GSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKR 359

Query: 407  IESAVLETLNKGFRTGDIFSTGKKLVGCTEIGEEVMKSLDSQKAAAV 267
            IE AVL  LNKGFRTGDI+S G KLVGC E+GEEV+KS+DS   A+V
Sbjct: 360  IEDAVLGALNKGFRTGDIYSAGTKLVGCKEMGEEVLKSVDSHVQASV 406