BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00006289
         (1191 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI19072.3| unnamed protein product [Vitis vinifera]              442   e-122
ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vin...   442   e-121
ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thalian...   441   e-121
gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidops...   441   e-121
ref|XP_002878877.1| holocarboxylase synthetase 1 [Arabidopsis ly...   439   e-121

>emb|CBI19072.3| unnamed protein product [Vitis vinifera]
          Length = 416

 Score =  442 bits (1137), Expect = e-122
 Identities = 221/336 (65%), Positives = 266/336 (79%)
 Frame = +3

Query: 117  SMENQPPSSTLLILHGKSSVETEFAQSLKTNNTLKLPESDGNASIFLQSLESTNLQNDTE 296
            +M++Q  S++LL+L GKSS E EFA+SLK  NTLKLP++ G  SI L       ++   E
Sbjct: 89   AMDSQ--STSLLVLCGKSSAEIEFAKSLKAKNTLKLPDN-GEVSILLHP----EIEKPEE 141

Query: 297  ETFDVELYMNSLSTYRFGRFLIWSPRLSSTQDLVSMNFNEIPVGSVCIADVQFKGRGRSK 476
            E F ++L+M+SLST  FGRFLIWSP+L ST D+VS NF E+P+G+VC+ADVQ KGRGRSK
Sbjct: 142  EPFQMDLFMSSLSTNTFGRFLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSK 201

Query: 477  NLWESPMGCLLFSFTLQMEDGRIVPLLQYVVSLAIVEAIKNVCDVNGFPCINVRIKWPND 656
            N WESP GCLLFSFTLQMEDGR VPLLQYVVSLAI EAIK++CD  G P ++V+IKWPND
Sbjct: 202  NKWESPRGCLLFSFTLQMEDGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPND 261

Query: 657  LYLDGVKVGGILCTSTYSLKKFNVSVGMGLNVDNEMPTTCLNKVFPSELSSSSCKFKREE 836
            LYL+G+KVGGILCTSTY  +KFNVS G+GLNVDNE PTTCLN V   ELSS + +F+RE+
Sbjct: 262  LYLNGLKVGGILCTSTYKSRKFNVSAGIGLNVDNEKPTTCLNAVL-RELSSVAYQFRRED 320

Query: 837  ILAAFFKRFESLYDVFINQGFQALEEMYCKAWLHSEQRVVIQXXXXXXXXXXXXTIQGLT 1016
            I+AAFF +FE+ + +FINQGFQ LEE+YCK WLHS QRV++Q            TIQGL 
Sbjct: 321  IVAAFFNKFETFFSLFINQGFQTLEELYCKTWLHSGQRVIVQENIEGQMVENVVTIQGLA 380

Query: 1017 SSGYLLAIDDDNQLYELHPDGNSFDFFKGLVRKKLE 1124
            SSGYLLAI DD Q+ ELHPDGNSFDFFKGL+R+KLE
Sbjct: 381  SSGYLLAIGDDGQMCELHPDGNSFDFFKGLIRRKLE 416


>ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vinifera]
          Length = 327

 Score =  442 bits (1136), Expect = e-121
 Identities = 219/329 (66%), Positives = 261/329 (79%)
 Frame = +3

Query: 138  SSTLLILHGKSSVETEFAQSLKTNNTLKLPESDGNASIFLQSLESTNLQNDTEETFDVEL 317
            S++LL+L GKSS E EFA+SLK  NTLKLP++ G  SI L       ++   EE F ++L
Sbjct: 5    STSLLVLCGKSSAEIEFAKSLKAKNTLKLPDN-GEVSILLHP----EIEKPEEEPFQMDL 59

Query: 318  YMNSLSTYRFGRFLIWSPRLSSTQDLVSMNFNEIPVGSVCIADVQFKGRGRSKNLWESPM 497
            +M+SLST  FGRFLIWSP+L ST D+VS NF E+P+G+VC+ADVQ KGRGRSKN WESP 
Sbjct: 60   FMSSLSTNTFGRFLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKNKWESPR 119

Query: 498  GCLLFSFTLQMEDGRIVPLLQYVVSLAIVEAIKNVCDVNGFPCINVRIKWPNDLYLDGVK 677
            GCLLFSFTLQMEDGR VPLLQYVVSLAI EAIK++CD  G P ++V+IKWPNDLYL+G+K
Sbjct: 120  GCLLFSFTLQMEDGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDLYLNGLK 179

Query: 678  VGGILCTSTYSLKKFNVSVGMGLNVDNEMPTTCLNKVFPSELSSSSCKFKREEILAAFFK 857
            VGGILCTSTY  +KFNVS G+GLNVDNE PTTCLN V   ELSS + +F+RE+I+AAFF 
Sbjct: 180  VGGILCTSTYKSRKFNVSAGIGLNVDNEKPTTCLNAVL-RELSSVAYQFRREDIVAAFFN 238

Query: 858  RFESLYDVFINQGFQALEEMYCKAWLHSEQRVVIQXXXXXXXXXXXXTIQGLTSSGYLLA 1037
            +FE+ + +FINQGFQ LEE+YCK WLHS QRV++Q            TIQGL SSGYLLA
Sbjct: 239  KFETFFSLFINQGFQTLEELYCKTWLHSGQRVIVQENIEGQMVENVVTIQGLASSGYLLA 298

Query: 1038 IDDDNQLYELHPDGNSFDFFKGLVRKKLE 1124
            I DD Q+ ELHPDGNSFDFFKGL+R+KLE
Sbjct: 299  IGDDGQMCELHPDGNSFDFFKGLIRRKLE 327


>ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thaliana]
            gi|30682866|ref|NP_850067.1| holocarboxylase synthase 1
            [Arabidopsis thaliana] gi|1688072|gb|AAC49706.1| biotin
            holocarboxylase synthetase [Arabidopsis thaliana]
            gi|20197509|gb|AAD31371.2| biotin holocarboxylase
            synthetase [Arabidopsis thaliana]
            gi|89000993|gb|ABD59086.1| At2g25710 [Arabidopsis
            thaliana] gi|330252646|gb|AEC07740.1| holocarboxylase
            synthase 1 [Arabidopsis thaliana]
            gi|330252647|gb|AEC07741.1| holocarboxylase synthase 1
            [Arabidopsis thaliana]
          Length = 367

 Score =  441 bits (1134), Expect = e-121
 Identities = 212/337 (62%), Positives = 270/337 (80%)
 Frame = +3

Query: 111  YSSMENQPPSSTLLILHGKSSVETEFAQSLKTNNTLKLPESDGNASIFLQSLESTNLQND 290
            +S+   +  +S  L+L GKSSVETE A+ LK  N+LKLP++    S+ L+S E+ NL  D
Sbjct: 34   FSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNT-KVSLILES-EAKNLVKD 91

Query: 291  TEETFDVELYMNSLSTYRFGRFLIWSPRLSSTQDLVSMNFNEIPVGSVCIADVQFKGRGR 470
             + +F++ L+MNS+ T+RFGRFLIWSPRLSST D+VS NF+E+PVGSVC+ D+QFKGRGR
Sbjct: 92   DDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGR 151

Query: 471  SKNLWESPMGCLLFSFTLQMEDGRIVPLLQYVVSLAIVEAIKNVCDVNGFPCINVRIKWP 650
            +KN+WESP GCL++SFTL+MEDGR+VPL+QYVVSLA+ EA+K+VCD  G P I+V+IKWP
Sbjct: 152  TKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWP 211

Query: 651  NDLYLDGVKVGGILCTSTYSLKKFNVSVGMGLNVDNEMPTTCLNKVFPSELSSSSCKFKR 830
            NDLY++G+KVGGILCTSTY  KKFNVSVG+GLNVDN  PTTCLN V    ++  S   KR
Sbjct: 212  NDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKG-MAPESNLLKR 270

Query: 831  EEILAAFFKRFESLYDVFINQGFQALEEMYCKAWLHSEQRVVIQXXXXXXXXXXXXTIQG 1010
            EEIL AFF +FE  +D+F++QGF++LEE+Y + WLHSEQRV+++            TIQG
Sbjct: 271  EEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQG 330

Query: 1011 LTSSGYLLAIDDDNQLYELHPDGNSFDFFKGLVRKKL 1121
            LTSSGYLLA+ DDNQ+YELHPDGNSFDFFKGLVR+K+
Sbjct: 331  LTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRKI 367


>gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidopsis thaliana]
          Length = 367

 Score =  441 bits (1134), Expect = e-121
 Identities = 212/337 (62%), Positives = 270/337 (80%)
 Frame = +3

Query: 111  YSSMENQPPSSTLLILHGKSSVETEFAQSLKTNNTLKLPESDGNASIFLQSLESTNLQND 290
            +S+   +  +S  L+L GKSSVETE A+ LK  N+LKLP++    S+ L+S E+ NL  D
Sbjct: 34   FSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNT-KVSLILES-EAKNLVKD 91

Query: 291  TEETFDVELYMNSLSTYRFGRFLIWSPRLSSTQDLVSMNFNEIPVGSVCIADVQFKGRGR 470
             + +F++ L+MNS+ T+RFGRFLIWSPRLSST D+VS NF+E+PVGSVC+ D+QFKGRGR
Sbjct: 92   DDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGR 151

Query: 471  SKNLWESPMGCLLFSFTLQMEDGRIVPLLQYVVSLAIVEAIKNVCDVNGFPCINVRIKWP 650
            +KN+WESP GCL++SFTL+MEDGR+VPL+QYVVSLA+ EA+K+VCD  G P I+V+IKWP
Sbjct: 152  TKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWP 211

Query: 651  NDLYLDGVKVGGILCTSTYSLKKFNVSVGMGLNVDNEMPTTCLNKVFPSELSSSSCKFKR 830
            NDLY++G+KVGGILCTSTY  KKFNVSVG+GLNVDN  PTTCLN V    ++  S   KR
Sbjct: 212  NDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKG-MAPESNLLKR 270

Query: 831  EEILAAFFKRFESLYDVFINQGFQALEEMYCKAWLHSEQRVVIQXXXXXXXXXXXXTIQG 1010
            EEIL AFF +FE  +D+F++QGF++LEE+Y + WLHSEQRV+++            TIQG
Sbjct: 271  EEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQG 330

Query: 1011 LTSSGYLLAIDDDNQLYELHPDGNSFDFFKGLVRKKL 1121
            LTSSGYLLA+ DDNQ+YELHPDGNSFDFFKGLVR+K+
Sbjct: 331  LTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRKI 367


>ref|XP_002878877.1| holocarboxylase synthetase 1 [Arabidopsis lyrata subsp. lyrata]
            gi|297324716|gb|EFH55136.1| holocarboxylase synthetase 1
            [Arabidopsis lyrata subsp. lyrata]
          Length = 365

 Score =  439 bits (1129), Expect = e-121
 Identities = 216/336 (64%), Positives = 271/336 (80%)
 Frame = +3

Query: 114  SSMENQPPSSTLLILHGKSSVETEFAQSLKTNNTLKLPESDGNASIFLQSLESTNLQNDT 293
            S+ME+   +S  L+L+GKSSVETE A+ LK  N+L LP++    S+FL+S E+ NL  D 
Sbjct: 36   SAMESD--ASCNLVLYGKSSVETEIAKCLKNKNSLTLPDNT-KVSLFLES-EAKNLVKD- 90

Query: 294  EETFDVELYMNSLSTYRFGRFLIWSPRLSSTQDLVSMNFNEIPVGSVCIADVQFKGRGRS 473
            + +F++ L+MNS+ T+RFGRFLIWSPRLSST D+VS NF+E+PVGSVC+ D+QFKGRGR+
Sbjct: 91   DGSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSQNFSELPVGSVCVTDIQFKGRGRT 150

Query: 474  KNLWESPMGCLLFSFTLQMEDGRIVPLLQYVVSLAIVEAIKNVCDVNGFPCINVRIKWPN 653
            KN+WESP GCL++SFTL+MEDGR+VPL+QYVVSLA+ EA+K+VCD  G P I+V+IKWPN
Sbjct: 151  KNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPN 210

Query: 654  DLYLDGVKVGGILCTSTYSLKKFNVSVGMGLNVDNEMPTTCLNKVFPSELSSSSCKFKRE 833
            DLYL+G+KVGGILCTSTY  KKF+VSVG+GLNVDNE PTTCLN V    +S  S   KRE
Sbjct: 211  DLYLNGLKVGGILCTSTYRSKKFHVSVGVGLNVDNEQPTTCLNAVLKG-MSPESTLLKRE 269

Query: 834  EILAAFFKRFESLYDVFINQGFQALEEMYCKAWLHSEQRVVIQXXXXXXXXXXXXTIQGL 1013
            EIL AFF +FE  +D+F++QGF++LEE+Y + WLHSEQRV ++            TIQGL
Sbjct: 270  EILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVTVEEKVEDQVVQNVVTIQGL 329

Query: 1014 TSSGYLLAIDDDNQLYELHPDGNSFDFFKGLVRKKL 1121
             SSGYLLAI DDNQ+YELHPDGNSFDFFKGLVR+K+
Sbjct: 330  ASSGYLLAIGDDNQMYELHPDGNSFDFFKGLVRRKI 365