BLASTX nr result
ID: Cimicifuga21_contig00006248
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cimicifuga21_contig00006248
(1222 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 373 e-101
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 373 e-101
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 372 e-101
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 372 e-101
ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222... 355 2e-95
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 373 bits (957), Expect = e-101
Identities = 187/339 (55%), Positives = 257/339 (75%), Gaps = 4/339 (1%)
Frame = +3
Query: 213 IFKHTKSSKN--LPPGPFPWPIIGNILSMGNKPHVSLAELAKTHGPLMSLRLGTQLLVVG 386
+ K + S K+ LPPGP PWPI+GN+L +GN PH+SLA ++++GPL+SLRLG+Q+LVV
Sbjct: 465 LLKDSSSLKSPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVA 524
Query: 387 SSPAAATEILKTNDRVLSARCVLHALPASPDKLH-FSVTWA-DCTDQWRYLRTLIRTELF 560
S+ +AA EILKT+DRVLS R V HA+PA +++ S+ WA +C D W+ LRT+ R ELF
Sbjct: 525 STSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELF 584
Query: 561 SPKVIESQAVIRDKKVGELVEFLHTKEGKLVNVGEVVFNTIFNLLSNLLVSKDLINMDEE 740
S KV+ESQA + +KKV E+V F+ TKEG+++ VGEVVF T+ N L+ +L+S+D I+ +++
Sbjct: 585 STKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDD 644
Query: 741 SEDGGLKGFVRKFVEVASSPNLADYYAMLSGLDLQGLNKKTKEMYAQISGIWEVIIKERR 920
+DGG+KG VRK + ++PNL D+Y + SGLDLQGLNKKTKE+ A+I +WE +I+ERR
Sbjct: 645 YKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERR 704
Query: 921 EGKGSDVARQKDLLDTMLERDLTNDHISYLFLELFLAGTDPSTSTVEWAMAELIKNPEAM 1100
EG D ++Q D L+ ++ ++D I+ LF+EL AG D S+ST+EWAMAELIK+PE+M
Sbjct: 705 EGASDDPSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESM 763
Query: 1101 RKVRIELEREINGTIIKESDLPRLPYLQAIVKEALRLHP 1217
+KV EL REI+ + K SDLP LPYLQA VKE LRLHP
Sbjct: 764 KKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHP 802
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 373 bits (957), Expect = e-101
Identities = 187/339 (55%), Positives = 257/339 (75%), Gaps = 4/339 (1%)
Frame = +3
Query: 213 IFKHTKSSKN--LPPGPFPWPIIGNILSMGNKPHVSLAELAKTHGPLMSLRLGTQLLVVG 386
+ K + S K+ LPPGP PWPI+GN+L +GN PH+SLA ++++GPL+SLRLG+Q+LVV
Sbjct: 32 LLKDSSSLKSPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVA 91
Query: 387 SSPAAATEILKTNDRVLSARCVLHALPASPDKLH-FSVTWA-DCTDQWRYLRTLIRTELF 560
S+ +AA EILKT+DRVLS R V HA+PA +++ S+ WA +C D W+ LRT+ R ELF
Sbjct: 92 STSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELF 151
Query: 561 SPKVIESQAVIRDKKVGELVEFLHTKEGKLVNVGEVVFNTIFNLLSNLLVSKDLINMDEE 740
S KV+ESQA + +KKV E+V F+ TKEG+++ VGEVVF T+ N L+ +L+S+D I+ +++
Sbjct: 152 STKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDD 211
Query: 741 SEDGGLKGFVRKFVEVASSPNLADYYAMLSGLDLQGLNKKTKEMYAQISGIWEVIIKERR 920
+DGG+KG VRK + ++PNL D+Y + SGLDLQGLNKKTKE+ A+I +WE +I+ERR
Sbjct: 212 YKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERR 271
Query: 921 EGKGSDVARQKDLLDTMLERDLTNDHISYLFLELFLAGTDPSTSTVEWAMAELIKNPEAM 1100
EG D ++Q D L+ ++ ++D I+ LF+EL AG D S+ST+EWAMAELIK+PE+M
Sbjct: 272 EGASDDPSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESM 330
Query: 1101 RKVRIELEREINGTIIKESDLPRLPYLQAIVKEALRLHP 1217
+KV EL REI+ + K SDLP LPYLQA VKE LRLHP
Sbjct: 331 KKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHP 369
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 372 bits (956), Expect = e-101
Identities = 187/339 (55%), Positives = 257/339 (75%), Gaps = 4/339 (1%)
Frame = +3
Query: 213 IFKHTKSSKN--LPPGPFPWPIIGNILSMGNKPHVSLAELAKTHGPLMSLRLGTQLLVVG 386
+ K + S K+ LPPGP PWPI+GN+L +GN PH+SLA ++++GPL+SLRLG+Q+LVV
Sbjct: 596 LLKDSSSLKSPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVA 655
Query: 387 SSPAAATEILKTNDRVLSARCVLHALPASPDKLH-FSVTWA-DCTDQWRYLRTLIRTELF 560
S+ +AA EILKT+DRVLS R V HA+PA +++ S+ WA +C W+ LRT+ R ELF
Sbjct: 656 STSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELF 715
Query: 561 SPKVIESQAVIRDKKVGELVEFLHTKEGKLVNVGEVVFNTIFNLLSNLLVSKDLINMDEE 740
S KV+ESQA + +KKV E+V F+ TKEG+++ VGEVVF T+ N L+ +L+S+D I+ +++
Sbjct: 716 STKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDD 775
Query: 741 SEDGGLKGFVRKFVEVASSPNLADYYAMLSGLDLQGLNKKTKEMYAQISGIWEVIIKERR 920
++DGG+KG VRK V ++PNL D+Y + SGLDLQGLNKKTKE+ A+I +WE +I+ERR
Sbjct: 776 NKDGGMKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERR 835
Query: 921 EGKGSDVARQKDLLDTMLERDLTNDHISYLFLELFLAGTDPSTSTVEWAMAELIKNPEAM 1100
EG D ++Q D L+ ++ ++D I+ LF+EL AG D S+ST+EWAMAELIK+PE+M
Sbjct: 836 EGASDDPSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESM 894
Query: 1101 RKVRIELEREINGTIIKESDLPRLPYLQAIVKEALRLHP 1217
+KV EL REI+ + K SDLP LPYLQA VKE LRLHP
Sbjct: 895 KKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHP 933
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 372 bits (956), Expect = e-101
Identities = 187/339 (55%), Positives = 257/339 (75%), Gaps = 4/339 (1%)
Frame = +3
Query: 213 IFKHTKSSKN--LPPGPFPWPIIGNILSMGNKPHVSLAELAKTHGPLMSLRLGTQLLVVG 386
+ K + S K+ LPPGP PWPI+GN+L +GN PH+SLA ++++GPL+SLRLG+Q+LVV
Sbjct: 32 LLKDSSSLKSPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVA 91
Query: 387 SSPAAATEILKTNDRVLSARCVLHALPASPDKLH-FSVTWA-DCTDQWRYLRTLIRTELF 560
S+ +AA EILKT+DRVLS R V HA+PA +++ S+ WA +C W+ LRT+ R ELF
Sbjct: 92 STSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELF 151
Query: 561 SPKVIESQAVIRDKKVGELVEFLHTKEGKLVNVGEVVFNTIFNLLSNLLVSKDLINMDEE 740
S KV+ESQA + +KKV E+V F+ TKEG+++ VGEVVF T+ N L+ +L+S+D I+ +++
Sbjct: 152 STKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDD 211
Query: 741 SEDGGLKGFVRKFVEVASSPNLADYYAMLSGLDLQGLNKKTKEMYAQISGIWEVIIKERR 920
++DGG+KG VRK V ++PNL D+Y + SGLDLQGLNKKTKE+ A+I +WE +I+ERR
Sbjct: 212 NKDGGMKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERR 271
Query: 921 EGKGSDVARQKDLLDTMLERDLTNDHISYLFLELFLAGTDPSTSTVEWAMAELIKNPEAM 1100
EG D ++Q D L+ ++ ++D I+ LF+EL AG D S+ST+EWAMAELIK+PE+M
Sbjct: 272 EGASDDPSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESM 330
Query: 1101 RKVRIELEREINGTIIKESDLPRLPYLQAIVKEALRLHP 1217
+KV EL REI+ + K SDLP LPYLQA VKE LRLHP
Sbjct: 331 KKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHP 369
>ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222845316|gb|EEE82863.1|
cytochrome P450 [Populus trichocarpa]
Length = 507
Score = 355 bits (910), Expect = 2e-95
Identities = 179/345 (51%), Positives = 246/345 (71%), Gaps = 10/345 (2%)
Frame = +3
Query: 216 FKHTKSSKNLPPGPFPWPIIGNILSMGNKPHVSLAELAKTHGPLMSLRLGTQLLVVGSSP 395
F+H SS PPGP+PWPI+GNIL +G+KPH++L AK HGP+ SLRLGTQL+VVGSS
Sbjct: 31 FRHN-SSPPFPPGPYPWPILGNILQLGDKPHITLTHFAKIHGPIFSLRLGTQLVVVGSSQ 89
Query: 396 AAATEILKTNDRVLSARCVLHALPASPDKLH-FSVTWA-DCTDQWRYLRTLIRTELFSPK 569
AAA ILKT+DR+LS R V H P+ +L+ S+ W +C ++WRYLRT+ ++ELFS K
Sbjct: 90 AAAIAILKTHDRILSGRHVPHMAPSKSSELNKLSLGWVVECNERWRYLRTICKSELFSLK 149
Query: 570 VIESQAVIRDKKVGELVEFLHTKEGKLVNVGEVVFNTIFNLLSNLLVSKDLINMDEESED 749
+ESQA IR++K E++ F++ EGK+V + EV T+FN+LSN+LVS+DL++++ ESED
Sbjct: 150 ALESQACIRERKAKEMIGFINKMEGKVVKIREVATATVFNMLSNILVSRDLVSLEHESED 209
Query: 750 GGLKGFVRKFVEVASSPNLADYYAMLSGLDLQGLNKKTKEMYAQISGIWEVIIKERREG- 926
GG+ ++ +AS+PN++D+Y +L LDLQGL KKT E++ + + E II+ERREG
Sbjct: 210 GGMSSVLKDIARLASTPNISDFYPILGPLDLQGLRKKTMELHRRSFNMCEAIIQERREGG 269
Query: 927 ----KGSDVARQKDLLDTMLERDLTNDHISYLFLELFLAGTDPSTSTVEWAMAELIKNPE 1094
G D +R++D LD ++ ++D I L +EL AGTD S+ST+EW MAELIKNP
Sbjct: 270 EGKRDGPDASRRRDFLDALILNGSSDDQIDILLMELLSAGTDTSSSTIEWTMAELIKNPR 329
Query: 1095 AMRKVRIELEREIN---GTIIKESDLPRLPYLQAIVKEALRLHPP 1220
++KV+ E+ IN T KES LP+L YLQA VKE LRLHPP
Sbjct: 330 CLKKVQEEIANVINMNRDTGFKESHLPQLTYLQACVKETLRLHPP 374