BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00006010
         (1363 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACY06328.1| S-adenosyl-L-methionine: caffeic acid 3-0-methylt...   417   e-114
sp|P28002.1|COMT1_MEDSA RecName: Full=Caffeic acid 3-O-methyltra...   416   e-114
ref|XP_003602396.1| Caffeic acid O-methyltransferase [Medicago t...   416   e-114
gb|AFK39235.1| unknown [Medicago truncatula]                          416   e-114
gb|AFK40372.1| unknown [Lotus japonicus]                              415   e-113

>gb|ACY06328.1| S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase [Medicago
            sativa]
          Length = 365

 Score =  417 bits (1073), Expect = e-114
 Identities = 201/349 (57%), Positives = 259/349 (74%)
 Frame = -2

Query: 1320 NEDQTYLFAMQLAVSSVMPMVMQSAIELNVFDIIAKAGEGAHLSPTEIASHLPTQNPEAP 1141
            ++++  LFAMQLA +SV+PM+++SA+EL++ +IIAKAG GA +SP EIAS LPT NP+AP
Sbjct: 15   SDEEANLFAMQLASASVLPMILKSALELDLLEIIAKAGPGAQISPIEIASQLPTTNPDAP 74

Query: 1140 DMLDRMLRLLANHSVLTISFVTRENGRVERLYGLAPVCKYFLPNEDGVSLTPLMLWSQDH 961
             MLDRMLRLLA +++LT S  T+++G+V+RLYGLA V KY + NEDGVS++ L L +QD 
Sbjct: 75   VMLDRMLRLLACYNILTCSVRTQQDGKVQRLYGLATVAKYLVKNEDGVSISALNLMNQDK 134

Query: 960  DNMRCWYRLKDAVLDGGIAFEKVHGLGLFEYNEKNPKFSELFNRTMYIHTTLIMKKVIGL 781
              M  WY LKDAVLDGGI F K +G+  FEY+  +P+F+++FN+ M  H+T+ MKK++  
Sbjct: 135  VLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILET 194

Query: 780  YNGFENVKEVIDVGGGIGTMSNIICSKYPTIKCINFDLPHVVETAPPHPGITHVGGDMFD 601
            Y GFE +K ++DVGGG G + N I SKYPTIK INFDLPHV+E AP +PG+ HVGGDMF 
Sbjct: 195  YTGFEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFV 254

Query: 600  SLPKGEAIFLKCILHDWDDEHCLKLLKNCYKALPDHGMVVVVDIILSXXXXXXXXXXXXX 421
            S+PK +A+F+K I HDW DEHCLK LKNCY+ALPD+G V+V + IL              
Sbjct: 255  SIPKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLATKGVV 314

Query: 420  EIDVFMMMQCPGGKERTAKEFEDLAKEAGFNGFKLVCGVSNYWAMEFTK 274
             IDV M+   PGGKERT KEFEDLAK AGF GFK+ C   N + MEF K
Sbjct: 315  HIDVIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIMEFLK 363


>sp|P28002.1|COMT1_MEDSA RecName: Full=Caffeic acid 3-O-methyltransferase; Short=CAOMT;
            Short=COMT; AltName:
            Full=S-adenosysl-L-methionine:caffeic acid
            3-O-methyltransferase gi|23200293|pdb|1KYW|A Chain A,
            Crystal Structure Analysis Of Caffeic
            Acid5-Hydroxyferulic Acid 35-O-Methyltransferase In
            Complex With 5- Hydroxyconiferaldehyde
            gi|23200294|pdb|1KYW|C Chain C, Crystal Structure
            Analysis Of Caffeic Acid5-Hydroxyferulic Acid
            35-O-Methyltransferase In Complex With 5-
            Hydroxyconiferaldehyde gi|23200295|pdb|1KYW|F Chain F,
            Crystal Structure Analysis Of Caffeic
            Acid5-Hydroxyferulic Acid 35-O-Methyltransferase In
            Complex With 5- Hydroxyconiferaldehyde
            gi|23200296|pdb|1KYZ|A Chain A, Crystal Structure
            Analysis Of Caffeic Acid5-Hydroxyferulic Acid
            35-O-Methyltransferase Ferulic Acid Complex
            gi|23200297|pdb|1KYZ|C Chain C, Crystal Structure
            Analysis Of Caffeic Acid5-Hydroxyferulic Acid
            35-O-Methyltransferase Ferulic Acid Complex
            gi|23200298|pdb|1KYZ|E Chain E, Crystal Structure
            Analysis Of Caffeic Acid5-Hydroxyferulic Acid
            35-O-Methyltransferase Ferulic Acid Complex
            gi|166420|gb|AAB46623.1| S-adenosyl-L-methionine: caffeic
            acid 3-0-methyltransferase [Medicago sativa]
          Length = 365

 Score =  416 bits (1070), Expect = e-114
 Identities = 201/349 (57%), Positives = 258/349 (73%)
 Frame = -2

Query: 1320 NEDQTYLFAMQLAVSSVMPMVMQSAIELNVFDIIAKAGEGAHLSPTEIASHLPTQNPEAP 1141
            ++++  LFAMQLA +SV+PM+++SA+EL++ +IIAKAG GA +SP EIAS LPT NP+AP
Sbjct: 15   SDEEANLFAMQLASASVLPMILKSALELDLLEIIAKAGPGAQISPIEIASQLPTTNPDAP 74

Query: 1140 DMLDRMLRLLANHSVLTISFVTRENGRVERLYGLAPVCKYFLPNEDGVSLTPLMLWSQDH 961
             MLDRMLRLLA + +LT S  T+++G+V+RLYGLA V KY + NEDGVS++ L L +QD 
Sbjct: 75   VMLDRMLRLLACYIILTCSVRTQQDGKVQRLYGLATVAKYLVKNEDGVSISALNLMNQDK 134

Query: 960  DNMRCWYRLKDAVLDGGIAFEKVHGLGLFEYNEKNPKFSELFNRTMYIHTTLIMKKVIGL 781
              M  WY LKDAVLDGGI F K +G+  FEY+  +P+F+++FN+ M  H+T+ MKK++  
Sbjct: 135  VLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILET 194

Query: 780  YNGFENVKEVIDVGGGIGTMSNIICSKYPTIKCINFDLPHVVETAPPHPGITHVGGDMFD 601
            Y GFE +K ++DVGGG G + N I SKYPTIK INFDLPHV+E AP +PG+ HVGGDMF 
Sbjct: 195  YTGFEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFV 254

Query: 600  SLPKGEAIFLKCILHDWDDEHCLKLLKNCYKALPDHGMVVVVDIILSXXXXXXXXXXXXX 421
            S+PK +A+F+K I HDW DEHCLK LKNCY+ALPD+G V+V + IL              
Sbjct: 255  SIPKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLATKGVV 314

Query: 420  EIDVFMMMQCPGGKERTAKEFEDLAKEAGFNGFKLVCGVSNYWAMEFTK 274
             IDV M+   PGGKERT KEFEDLAK AGF GFK+ C   N + MEF K
Sbjct: 315  HIDVIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIMEFLK 363


>ref|XP_003602396.1| Caffeic acid O-methyltransferase [Medicago truncatula]
            gi|355491444|gb|AES72647.1| Caffeic acid
            O-methyltransferase [Medicago truncatula]
          Length = 365

 Score =  416 bits (1069), Expect = e-114
 Identities = 201/349 (57%), Positives = 258/349 (73%)
 Frame = -2

Query: 1320 NEDQTYLFAMQLAVSSVMPMVMQSAIELNVFDIIAKAGEGAHLSPTEIASHLPTQNPEAP 1141
            ++++  LFAMQLA +SV+PMV++SA+EL++ +IIAKAG GA +SP EIAS LPT NPEAP
Sbjct: 15   SDEEANLFAMQLASASVLPMVLKSALELDLLEIIAKAGPGAQISPIEIASQLPTTNPEAP 74

Query: 1140 DMLDRMLRLLANHSVLTISFVTRENGRVERLYGLAPVCKYFLPNEDGVSLTPLMLWSQDH 961
             MLDR+LRLLA +++LT S  T+++G+V+RLYGLA V KY + NEDGVS++ L L +QD 
Sbjct: 75   VMLDRILRLLACYNILTCSVRTQQDGKVQRLYGLATVAKYLVKNEDGVSISALNLMNQDK 134

Query: 960  DNMRCWYRLKDAVLDGGIAFEKVHGLGLFEYNEKNPKFSELFNRTMYIHTTLIMKKVIGL 781
              M  WY LKDAVLDGGI F K +G+  FEY+  +P+F+++FN+ M  H+T+ MKK++  
Sbjct: 135  VLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILET 194

Query: 780  YNGFENVKEVIDVGGGIGTMSNIICSKYPTIKCINFDLPHVVETAPPHPGITHVGGDMFD 601
            Y GFE +K ++DVGGG G + N I SKYPTIK INFDLPHV+E AP +PG+ HVGGDMF 
Sbjct: 195  YTGFEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFV 254

Query: 600  SLPKGEAIFLKCILHDWDDEHCLKLLKNCYKALPDHGMVVVVDIILSXXXXXXXXXXXXX 421
            S+PK +A+F+K I HDW DEHCLK LKNCY+ALPD+G V+V + IL              
Sbjct: 255  SIPKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLATKGVV 314

Query: 420  EIDVFMMMQCPGGKERTAKEFEDLAKEAGFNGFKLVCGVSNYWAMEFTK 274
             ID  M+   PGGKERT KEFEDLAK AGF GFK+ C   N + MEF K
Sbjct: 315  HIDAIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIMEFLK 363


>gb|AFK39235.1| unknown [Medicago truncatula]
          Length = 365

 Score =  416 bits (1068), Expect = e-114
 Identities = 201/349 (57%), Positives = 258/349 (73%)
 Frame = -2

Query: 1320 NEDQTYLFAMQLAVSSVMPMVMQSAIELNVFDIIAKAGEGAHLSPTEIASHLPTQNPEAP 1141
            ++++  LFAMQLA +SV+PMV++SA+EL++ +IIAKAG GA +SP EIAS LPT NPEAP
Sbjct: 15   SDEEANLFAMQLASASVLPMVLKSALELDLLEIIAKAGPGAQISPIEIASQLPTTNPEAP 74

Query: 1140 DMLDRMLRLLANHSVLTISFVTRENGRVERLYGLAPVCKYFLPNEDGVSLTPLMLWSQDH 961
             MLDR+LRLLA +++LT S  T+++G+V+RLYGLA V KY + NEDGVS++ L L +QD 
Sbjct: 75   VMLDRILRLLACYNILTCSVRTQQDGKVQRLYGLATVAKYLVKNEDGVSISALNLMNQDK 134

Query: 960  DNMRCWYRLKDAVLDGGIAFEKVHGLGLFEYNEKNPKFSELFNRTMYIHTTLIMKKVIGL 781
              M  WY LKDAVLDGGI F K +G+  FEY+  +P+F+++FN+ M  H+T+ MKK++  
Sbjct: 135  VLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILET 194

Query: 780  YNGFENVKEVIDVGGGIGTMSNIICSKYPTIKCINFDLPHVVETAPPHPGITHVGGDMFD 601
            Y GFE +K ++DVGGG G + N I SKYPTIK INFDLPHV+E AP +PG+ HVGGDMF 
Sbjct: 195  YTGFEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFV 254

Query: 600  SLPKGEAIFLKCILHDWDDEHCLKLLKNCYKALPDHGMVVVVDIILSXXXXXXXXXXXXX 421
            S+PK +A+F+K I HDW DEHCLK LKNCY+ALPD+G V+V + IL              
Sbjct: 255  SIPKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLATKGVV 314

Query: 420  EIDVFMMMQCPGGKERTAKEFEDLAKEAGFNGFKLVCGVSNYWAMEFTK 274
             ID  M+   PGGKERT KEFEDLAK AGF GFK+ C   N + MEF K
Sbjct: 315  HIDAIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIMEFFK 363


>gb|AFK40372.1| unknown [Lotus japonicus]
          Length = 365

 Score =  415 bits (1067), Expect = e-113
 Identities = 200/349 (57%), Positives = 259/349 (74%)
 Frame = -2

Query: 1320 NEDQTYLFAMQLAVSSVMPMVMQSAIELNVFDIIAKAGEGAHLSPTEIASHLPTQNPEAP 1141
            ++++  LFAMQLA +SV+PMV++SAIEL++ + IAKAG GA LSP EIAS LPT NP+AP
Sbjct: 15   SDEEANLFAMQLASASVLPMVLKSAIELDLLEFIAKAGTGAQLSPAEIASQLPTTNPDAP 74

Query: 1140 DMLDRMLRLLANHSVLTISFVTRENGRVERLYGLAPVCKYFLPNEDGVSLTPLMLWSQDH 961
             +LDRMLRLLA +++LT S  T ++G+V+RLYGLAPV KY + NEDGVS++ L L +QD 
Sbjct: 75   TVLDRMLRLLACYNILTCSVRTEQDGKVQRLYGLAPVAKYLVKNEDGVSISALNLMNQDK 134

Query: 960  DNMRCWYRLKDAVLDGGIAFEKVHGLGLFEYNEKNPKFSELFNRTMYIHTTLIMKKVIGL 781
              M  WY LKDAVL+GGI F K +G+  FEY+  +P+F+++FN+ M  H+T+ MKK++  
Sbjct: 135  VLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILET 194

Query: 780  YNGFENVKEVIDVGGGIGTMSNIICSKYPTIKCINFDLPHVVETAPPHPGITHVGGDMFD 601
            Y GFE +K ++DVGGG G + N+I SKYPTIK INFDLPHV+E AP +PG+ HVGGDMF 
Sbjct: 195  YTGFEGLKSLVDVGGGTGAVINMIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFV 254

Query: 600  SLPKGEAIFLKCILHDWDDEHCLKLLKNCYKALPDHGMVVVVDIILSXXXXXXXXXXXXX 421
            S+PK +A+F+K I HDW DEHCLK LKNCY+ALPD+G V+V + IL              
Sbjct: 255  SVPKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLATKGVV 314

Query: 420  EIDVFMMMQCPGGKERTAKEFEDLAKEAGFNGFKLVCGVSNYWAMEFTK 274
             IDV M+   PGGKERT KEFE LAK AGF GF+++C   N + MEF K
Sbjct: 315  HIDVIMLAHNPGGKERTEKEFEALAKGAGFQGFQVLCCAFNSYVMEFLK 363