BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00004927
         (1184 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ...   163   1e-73
emb|CBI19438.3| unnamed protein product [Vitis vinifera]              163   1e-73
ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   160   6e-72
emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]                157   3e-71
emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]                157   3e-71

>ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
          Length = 498

 Score =  163 bits (413), Expect(2) = 1e-73
 Identities = 88/175 (50%), Positives = 120/175 (68%), Gaps = 1/175 (0%)
 Frame = -3

Query: 1176 LQGLEKRMVISGRERDCFMQNLVDEIREKKHVSTKDGQENKTMIDVLLSKQEADPQYYSD 997
            L+G EKR++   + RD F+QNL++E R K    +K  +  KTMI+VLLS QE +P+ Y+D
Sbjct: 228  LKGTEKRLMELQKMRDSFIQNLIEEHRIK---GSKCERRPKTMIEVLLSLQETEPENYTD 284

Query: 996  EIIKGILSTLLVAGTDTTSATMEWAMSLXXXXXXXXXXXXXXXXXEWAMSLLLNHPEILK 817
            EII G++ ++L AGTDT++ TMEWA+SL                       LLN PE+LK
Sbjct: 285  EIIGGLMLSMLTAGTDTSAGTMEWALSL-----------------------LLNSPEVLK 321

Query: 816  KAREEIDIHVGHERLLEESDLPNLPYLHNIISETLRLYPTGPVVI-HESSKDCTV 655
            KA++EID+H+GH+RL+EE DL  LPYL +II ETLR+YP GP++I HESSK+C V
Sbjct: 322  KAQQEIDVHLGHDRLIEEVDLAQLPYLRSIIKETLRMYPAGPLLIPHESSKECFV 376



 Score =  140 bits (354), Expect(2) = 1e-73
 Identities = 61/107 (57%), Positives = 79/107 (73%)
 Frame = -2

Query: 613 GGFHVPSGTVLQMNFWAVHRDPNAWVDPKKFNPXXXXXXXXXXXXXKWIPFGSGRRRCPG 434
           GGF +P GT+L +N WA+H DP  W +P KF P             +++PFGSGRR CPG
Sbjct: 377 GGFRIPPGTMLLVNVWAIHNDPKIWAEPTKFKPERFEGEEGERDGLRFLPFGSGRRGCPG 436

Query: 433 EALAVRMVGLALGSLIQCFEWERFGEEMVDMSEGIGLTLPKAKPLVA 293
           E LA+RMVGLA+GSLIQCF+WER  ++MVDM+EG GL++PKA+PL+A
Sbjct: 437 EGLAIRMVGLAMGSLIQCFDWERVDQQMVDMTEGHGLSIPKAQPLLA 483


>emb|CBI19438.3| unnamed protein product [Vitis vinifera]
          Length = 476

 Score =  163 bits (413), Expect(2) = 1e-73
 Identities = 88/175 (50%), Positives = 120/175 (68%), Gaps = 1/175 (0%)
 Frame = -3

Query: 1176 LQGLEKRMVISGRERDCFMQNLVDEIREKKHVSTKDGQENKTMIDVLLSKQEADPQYYSD 997
            L+G EKR++   + RD F+QNL++E R K    +K  +  KTMI+VLLS QE +P+ Y+D
Sbjct: 206  LKGTEKRLMELQKMRDSFIQNLIEEHRIK---GSKCERRPKTMIEVLLSLQETEPENYTD 262

Query: 996  EIIKGILSTLLVAGTDTTSATMEWAMSLXXXXXXXXXXXXXXXXXEWAMSLLLNHPEILK 817
            EII G++ ++L AGTDT++ TMEWA+SL                       LLN PE+LK
Sbjct: 263  EIIGGLMLSMLTAGTDTSAGTMEWALSL-----------------------LLNSPEVLK 299

Query: 816  KAREEIDIHVGHERLLEESDLPNLPYLHNIISETLRLYPTGPVVI-HESSKDCTV 655
            KA++EID+H+GH+RL+EE DL  LPYL +II ETLR+YP GP++I HESSK+C V
Sbjct: 300  KAQQEIDVHLGHDRLIEEVDLAQLPYLRSIIKETLRMYPAGPLLIPHESSKECFV 354



 Score =  140 bits (354), Expect(2) = 1e-73
 Identities = 61/107 (57%), Positives = 79/107 (73%)
 Frame = -2

Query: 613 GGFHVPSGTVLQMNFWAVHRDPNAWVDPKKFNPXXXXXXXXXXXXXKWIPFGSGRRRCPG 434
           GGF +P GT+L +N WA+H DP  W +P KF P             +++PFGSGRR CPG
Sbjct: 355 GGFRIPPGTMLLVNVWAIHNDPKIWAEPTKFKPERFEGEEGERDGLRFLPFGSGRRGCPG 414

Query: 433 EALAVRMVGLALGSLIQCFEWERFGEEMVDMSEGIGLTLPKAKPLVA 293
           E LA+RMVGLA+GSLIQCF+WER  ++MVDM+EG GL++PKA+PL+A
Sbjct: 415 EGLAIRMVGLAMGSLIQCFDWERVDQQMVDMTEGHGLSIPKAQPLLA 461


>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score =  160 bits (406), Expect(2) = 6e-72
 Identities = 84/175 (48%), Positives = 122/175 (69%), Gaps = 1/175 (0%)
 Frame = -3

Query: 1176 LQGLEKRMVISGRERDCFMQNLVDEIREKKHVSTKDGQENKTMIDVLLSKQEADPQYYSD 997
            L+G E+R++   + R+ F+QNL++E R K   S  +G++ KTMI+VLLS QE +P+YY+D
Sbjct: 229  LKGTERRLIKLQKRRESFIQNLIEEHRRKG--SNCEGRQ-KTMIEVLLSLQETEPEYYTD 285

Query: 996  EIIKGILSTLLVAGTDTTSATMEWAMSLXXXXXXXXXXXXXXXXXEWAMSLLLNHPEILK 817
            EII+G++ ++L  GTDT++ TMEWA+SL                       LLN+P++LK
Sbjct: 286  EIIRGLMLSMLTGGTDTSAGTMEWALSL-----------------------LLNNPKVLK 322

Query: 816  KAREEIDIHVGHERLLEESDLPNLPYLHNIISETLRLYPTGPVVI-HESSKDCTV 655
            KA +EID  +GH+RL+EE DL  LPYL +II ETLR+YP GP+++ HESSK+C+V
Sbjct: 323  KAHQEIDDRLGHDRLIEELDLAQLPYLRSIIKETLRMYPAGPLLVPHESSKECSV 377



 Score =  137 bits (346), Expect(2) = 6e-72
 Identities = 64/107 (59%), Positives = 78/107 (72%)
 Frame = -2

Query: 613 GGFHVPSGTVLQMNFWAVHRDPNAWVDPKKFNPXXXXXXXXXXXXXKWIPFGSGRRRCPG 434
           GGF +P GT+L +N WA+  D   W DP +F P             K++PFGSGRR CPG
Sbjct: 378 GGFRIPQGTMLLVNLWAIQSDHKIWGDPTEFRPERFEGVEGDRDGFKFVPFGSGRRGCPG 437

Query: 433 EALAVRMVGLALGSLIQCFEWERFGEEMVDMSEGIGLTLPKAKPLVA 293
           EALA+R+VGLALGSLIQCF+WER  E+MVDM+EG GLTLPKA+PL+A
Sbjct: 438 EALAIRIVGLALGSLIQCFDWERVDEQMVDMTEGGGLTLPKAQPLLA 484


>emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]
          Length = 520

 Score =  157 bits (398), Expect(3) = 3e-71
 Identities = 81/175 (46%), Positives = 116/175 (66%), Gaps = 1/175 (0%)
 Frame = -3

Query: 1176 LQGLEKRMVISGRERDCFMQNLVDEIREKKHVSTKDGQENKTMIDVLLSKQEADPQYYSD 997
            ++GLEK+++    +RD F Q L+D++R+ K   T+D  + KTMI++LLS QE +P+YY+D
Sbjct: 249  VKGLEKKLIKLQEKRDVFFQGLIDQLRKSK--GTEDVNKKKTMIELLLSLQETEPEYYTD 306

Query: 996  EIIKGILSTLLVAGTDTTSATMEWAMSLXXXXXXXXXXXXXXXXXEWAMSLLLNHPEILK 817
             +I+  +  LL AG+DT++ TMEW MSL                       LLNHP++LK
Sbjct: 307  AMIRSFVLVLLAAGSDTSAGTMEWVMSL-----------------------LLNHPQVLK 343

Query: 816  KAREEIDIHVGHERLLEESDLPNLPYLHNIISETLRLYPTGPVVI-HESSKDCTV 655
            KA+ EID  +G   L++ESD+PNLPYL  II+ETLRLYP GP+++ HE+S DC V
Sbjct: 344  KAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLYPAGPLLVPHEASSDCVV 398



 Score =  136 bits (342), Expect(3) = 3e-71
 Identities = 62/107 (57%), Positives = 77/107 (71%)
 Frame = -2

Query: 613 GGFHVPSGTVLQMNFWAVHRDPNAWVDPKKFNPXXXXXXXXXXXXXKWIPFGSGRRRCPG 434
           GG++VP GT+L +N WA+H DP  W +P+ F P             K +PFGSGRR CPG
Sbjct: 399 GGYNVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTRDGFKLLPFGSGRRSCPG 458

Query: 433 EALAVRMVGLALGSLIQCFEWERFGEEMVDMSEGIGLTLPKAKPLVA 293
           E LAVRM+G+ LGS+IQCF+WER  EE+VDM+EG GLT+PKA PLVA
Sbjct: 459 EGLAVRMLGMTLGSIIQCFDWERTSEELVDMTEGPGLTMPKAIPLVA 505



 Score = 23.9 bits (50), Expect(3) = 3e-71
 Identities = 8/18 (44%), Positives = 14/18 (77%)
 Frame = -3

Query: 225 LVAMCKPRLSMKHIIASI 172
           LVA CKPR+ M ++++ +
Sbjct: 503 LVAKCKPRVEMTNLLSEL 520


>emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]
          Length = 505

 Score =  157 bits (398), Expect(3) = 3e-71
 Identities = 81/175 (46%), Positives = 116/175 (66%), Gaps = 1/175 (0%)
 Frame = -3

Query: 1176 LQGLEKRMVISGRERDCFMQNLVDEIREKKHVSTKDGQENKTMIDVLLSKQEADPQYYSD 997
            ++GLEK+++    +RD F Q L+D++R+ K   T+D  + KTMI++LLS QE +P+YY+D
Sbjct: 234  VKGLEKKLIKLQEKRDVFFQGLIDQLRKSK--GTEDVNKKKTMIELLLSLQETEPEYYTD 291

Query: 996  EIIKGILSTLLVAGTDTTSATMEWAMSLXXXXXXXXXXXXXXXXXEWAMSLLLNHPEILK 817
             +I+  +  LL AG+DT++ TMEW MSL                       LLNHP++LK
Sbjct: 292  AMIRSFVLVLLAAGSDTSAGTMEWVMSL-----------------------LLNHPQVLK 328

Query: 816  KAREEIDIHVGHERLLEESDLPNLPYLHNIISETLRLYPTGPVVI-HESSKDCTV 655
            KA+ EID  +G   L++ESD+PNLPYL  II+ETLRLYP GP+++ HE+S DC V
Sbjct: 329  KAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLYPAGPLLVPHEASSDCVV 383



 Score =  136 bits (342), Expect(3) = 3e-71
 Identities = 62/107 (57%), Positives = 77/107 (71%)
 Frame = -2

Query: 613 GGFHVPSGTVLQMNFWAVHRDPNAWVDPKKFNPXXXXXXXXXXXXXKWIPFGSGRRRCPG 434
           GG++VP GT+L +N WA+H DP  W +P+ F P             K +PFGSGRR CPG
Sbjct: 384 GGYNVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTRDGFKLLPFGSGRRSCPG 443

Query: 433 EALAVRMVGLALGSLIQCFEWERFGEEMVDMSEGIGLTLPKAKPLVA 293
           E LAVRM+G+ LGS+IQCF+WER  EE+VDM+EG GLT+PKA PLVA
Sbjct: 444 EGLAVRMLGMTLGSIIQCFDWERTSEELVDMTEGPGLTMPKAIPLVA 490



 Score = 23.9 bits (50), Expect(3) = 3e-71
 Identities = 8/18 (44%), Positives = 14/18 (77%)
 Frame = -3

Query: 225 LVAMCKPRLSMKHIIASI 172
           LVA CKPR+ M ++++ +
Sbjct: 488 LVAKCKPRVEMTNLLSEL 505