BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00003667
         (1878 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281332.1| PREDICTED: secologanin synthase [Vitis vinif...   645   0.0  
ref|XP_003635295.1| PREDICTED: secologanin synthase-like [Vitis ...   640   0.0  
ref|XP_004144024.1| PREDICTED: secologanin synthase-like [Cucumi...   632   e-178
ref|XP_003633108.1| PREDICTED: LOW QUALITY PROTEIN: secologanin ...   625   e-177
dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       625   e-177

>ref|XP_002281332.1| PREDICTED: secologanin synthase [Vitis vinifera]
            gi|296081714|emb|CBI20719.3| unnamed protein product
            [Vitis vinifera]
          Length = 512

 Score =  645 bits (1665), Expect = 0.0
 Identities = 312/500 (62%), Positives = 400/500 (80%), Gaps = 2/500 (0%)
 Frame = +2

Query: 134  VKVFHSLWLKPKSLEKQLKQQGIKGSPYKFLNGNLRDYTGMFKEALSKPLDLTHSIVPRV 313
            ++  +++W +PKSLEKQL+QQGI+G+ YK + G+++     F+EA SKP+ L HSIVPRV
Sbjct: 22   LRAVYTIWWRPKSLEKQLRQQGIRGTRYKPMYGDMKALKLSFQEAQSKPMTLNHSIVPRV 81

Query: 314  IPYFNQLVQTHGKISLFWLGTTPTLIIKDPELMKDVLSDKSWRFQKPPENPHIKLLTMGL 493
            IP+F+Q+ Q +GKIS+ W+ T P ++I DPEL++ +L+DK+ +FQKPP NP + LLT+GL
Sbjct: 82   IPFFHQMFQNYGKISMSWIFTRPRVMIVDPELIRMILADKNGQFQKPPLNPLVDLLTLGL 141

Query: 494  STQEGEKWVKHRKIVMPAFHLEKLKGMMPAFSISCSTLIEKWKKSVSPQGSLELDVWPDI 673
            ST EGE+W K RK++ PAFH+EKL GM+PAFS+SC  LIE+WK  V PQG+ ELDV P+ 
Sbjct: 142  STLEGEQWAKRRKLITPAFHVEKLMGMVPAFSMSCCNLIERWKNWVGPQGTYELDVMPEF 201

Query: 674  QTFTSDVISRTAFGSSYEEGKKIFELQKEQAVLVLEAFESVYIPGFRFLPTKKNKRRMNL 853
            Q  T DVISR AFGSSYEEGKK+FELQKEQAVLV+EA  ++Y+PGFRF+PT KN+RR ++
Sbjct: 202  QNVTGDVISRAAFGSSYEEGKKVFELQKEQAVLVIEASRAIYLPGFRFVPTVKNRRRYHI 261

Query: 854  DKEIKANLRGLIERKMLAMRNGESG-DDDLIGMLLQSNNTNYLQADDGPKVTGLTINEVI 1030
            D EIKA LR +I+RK  AM+NG+SG +DDL+G+LLQ       + D+      + I ++I
Sbjct: 262  DNEIKAMLRSMIDRKKQAMKNGDSGYNDDLLGLLLQLTE----EIDN-----EMRIEDLI 312

Query: 1031 EECKLFYFAGQETSSTWLTWTMIVLSMHPSWQEKAREEVLQVCGKNMPRFENLNHFKIVT 1210
            EECKLFYFAGQET++  LTWTMI+LSM+P WQ+KAREEVLQ+CGK +P  E + H KIV+
Sbjct: 313  EECKLFYFAGQETTANLLTWTMILLSMNPKWQDKAREEVLQICGKKIPDLEAIKHLKIVS 372

Query: 1211 MILYEVLRLYPPVIHQFRYTSTRMTVGDISLPAGVHLILPTLLIHHDREVWGDDAEDFRP 1390
            MIL+EVLRLYP V++  RYT  R  V  +S+PAGV L LPT+L+HH  E WGDD E+F+P
Sbjct: 373  MILHEVLRLYPSVVNLLRYTHKRTDVAGLSIPAGVELYLPTILLHHSPEYWGDDVEEFKP 432

Query: 1391 ERFSEGISKASK-DQVAFFPFGWGPRLCVGQNFAMIEAKMALAMILQHFEFELSSTYVHA 1567
            ERFSEG+SKASK DQ+AF+PFGWGPR+C+GQ+FAMIEAKMALAMILQ+F FELS TY HA
Sbjct: 433  ERFSEGVSKASKGDQIAFYPFGWGPRICLGQSFAMIEAKMALAMILQNFWFELSPTYTHA 492

Query: 1568 PCTVITLQPQHGAQIILHHL 1627
            P TVITLQPQ+GA IILH +
Sbjct: 493  PYTVITLQPQYGAPIILHQI 512


>ref|XP_003635295.1| PREDICTED: secologanin synthase-like [Vitis vinifera]
            gi|297741788|emb|CBI33075.3| unnamed protein product
            [Vitis vinifera]
          Length = 513

 Score =  640 bits (1651), Expect = 0.0
 Identities = 308/498 (61%), Positives = 392/498 (78%), Gaps = 1/498 (0%)
 Frame = +2

Query: 137  KVFHSLWLKPKSLEKQLKQQGIKGSPYKFLNGNLRDYTGMFKEALSKPLDLTHSIVPRVI 316
            +V +S+W +PKSLEKQL++QGI+G+ YK L G+ +     F EA SKP+ L HSIVPRV+
Sbjct: 25   RVVYSVWWRPKSLEKQLRRQGIRGTRYKLLFGDAKAMKQSFMEARSKPMALNHSIVPRVL 84

Query: 317  PYFNQLVQTHGKISLFWLGTTPTLIIKDPELMKDVLSDKSWRFQKPPENPHIKLLTMGLS 496
            P+++++ Q +GK+S+ W  TTP ++I +PELM+ +L+ K+  FQ+ P NP   LLT GLS
Sbjct: 85   PFYHEIAQKYGKVSVSWNFTTPRVLIVEPELMRLILTSKNGHFQRLPGNPLGYLLTRGLS 144

Query: 497  TQEGEKWVKHRKIVMPAFHLEKLKGMMPAFSISCSTLIEKWKKSVSPQGSLELDVWPDIQ 676
              +GEKW K RK++ PAFH EKLKGM+PAFS+SC  LIE+WK  V+PQG+ ELD+ P+ Q
Sbjct: 145  YLQGEKWAKRRKLLTPAFHFEKLKGMVPAFSVSCRKLIERWKNLVAPQGTYELDMMPEFQ 204

Query: 677  TFTSDVISRTAFGSSYEEGKKIFELQKEQAVLVLEAFESVYIPGFRFLPTKKNKRRMNLD 856
              T DVIS+ AFGS+YEEGKK+FELQKEQAVLV+EAF + YIPGFRF+P  KNK+R  +D
Sbjct: 205  NLTGDVISQVAFGSNYEEGKKVFELQKEQAVLVMEAFRTFYIPGFRFVPIGKNKKRYYID 264

Query: 857  KEIKANLRGLIERKMLAMRNGESGDDDLIGMLLQSNNTNYLQADDGPKVTGLTINEVIEE 1036
             EIKA L+ +I ++   M+ G+ G+DDL+G+LLQ       Q D     + +TI +VIEE
Sbjct: 265  SEIKAILKKIILKRKQTMKPGDLGNDDLLGLLLQCQE----QTD-----SEMTIEDVIEE 315

Query: 1037 CKLFYFAGQETSSTWLTWTMIVLSMHPSWQEKAREEVLQVCGKNMPRFENLNHFKIVTMI 1216
            CKLFYFAGQET++ WLTWT+++LSMHP+WQEKAREEVLQ+CGK MP  E +NH KIV+MI
Sbjct: 316  CKLFYFAGQETTANWLTWTILLLSMHPNWQEKAREEVLQICGKKMPDIEAINHLKIVSMI 375

Query: 1217 LYEVLRLYPPVIHQFRYTSTRMTVGDISLPAGVHLILPTLLIHHDREVWGDDAEDFRPER 1396
            L+EVLRLYPPV  QFR+T  R+ +  + +PAGV+L+LPTLL+HH  E WGDD E+F+PER
Sbjct: 376  LHEVLRLYPPVTQQFRHTCERINIAGMCIPAGVNLVLPTLLLHHSPEYWGDDVEEFKPER 435

Query: 1397 FSEGISKASK-DQVAFFPFGWGPRLCVGQNFAMIEAKMALAMILQHFEFELSSTYVHAPC 1573
            FSEG+SKASK DQ+AF+PFGWG R+C+GQ FAMIEAKMALAMILQHF FELS TY HAP 
Sbjct: 436  FSEGVSKASKGDQIAFYPFGWGHRICLGQGFAMIEAKMALAMILQHFWFELSPTYTHAPH 495

Query: 1574 TVITLQPQHGAQIILHHL 1627
            TVITLQPQHGA IILH +
Sbjct: 496  TVITLQPQHGAPIILHEI 513


>ref|XP_004144024.1| PREDICTED: secologanin synthase-like [Cucumis sativus]
            gi|449500470|ref|XP_004161105.1| PREDICTED: secologanin
            synthase-like [Cucumis sativus]
          Length = 511

 Score =  632 bits (1630), Expect = e-178
 Identities = 300/497 (60%), Positives = 388/497 (78%)
 Frame = +2

Query: 137  KVFHSLWLKPKSLEKQLKQQGIKGSPYKFLNGNLRDYTGMFKEALSKPLDLTHSIVPRVI 316
            K FHS+W KP S+E+ LK+QGI+G+ YK L G++++Y  M  EA S P++L H IV RV 
Sbjct: 20   KAFHSIWWKPISVERHLKRQGIRGNAYKPLVGDVKEYVRMMTEAWSTPMNLDHQIVQRVD 79

Query: 317  PYFNQLVQTHGKISLFWLGTTPTLIIKDPELMKDVLSDKSWRFQKPPENPHIKLLTMGLS 496
            P+     + +GKIS+ W GT+P LI+KDPE+MK+VL++K   F+KPP +P I  LT GL+
Sbjct: 80   PFTAVNAEKYGKISMCWFGTSPRLIVKDPEMMKEVLTNKVGSFEKPPLSPLILTLTRGLT 139

Query: 497  TQEGEKWVKHRKIVMPAFHLEKLKGMMPAFSISCSTLIEKWKKSVSPQGSLELDVWPDIQ 676
            T +G +W KH++I+ PAFHLE+LKGMM AF+ISCS +IE+WK+  + Q S+E+DVWP++Q
Sbjct: 140  TLKGNEWAKHKRIINPAFHLERLKGMMAAFNISCSNMIEEWKEKAANQDSVEVDVWPELQ 199

Query: 677  TFTSDVISRTAFGSSYEEGKKIFELQKEQAVLVLEAFESVYIPGFRFLPTKKNKRRMNLD 856
              T DVISR AFGS++EEGKKIFE  K+  +L LEA +++Y+PGFRF+PT KN++R  L+
Sbjct: 200  RLTGDVISRAAFGSNFEEGKKIFEHLKQLTLLTLEAMQTLYLPGFRFIPTAKNRKRERLN 259

Query: 857  KEIKANLRGLIERKMLAMRNGESGDDDLIGMLLQSNNTNYLQADDGPKVTGLTINEVIEE 1036
            KEIK  +R LI RK  +M N E   DDL+ +LLQS      Q  DG     LTI+EV+EE
Sbjct: 260  KEIKTMIRSLILRKESSMSNQEDNADDLLSLLLQSKKKENQQEGDG-----LTIDEVMEE 314

Query: 1037 CKLFYFAGQETSSTWLTWTMIVLSMHPSWQEKAREEVLQVCGKNMPRFENLNHFKIVTMI 1216
            CK FY AGQET+++ L WT+IVL+MH  WQEKAR+EVLQ CGKN P FE+LNHFKIVTMI
Sbjct: 315  CKQFYLAGQETTASLLIWTVIVLAMHSDWQEKARQEVLQFCGKNEPTFESLNHFKIVTMI 374

Query: 1217 LYEVLRLYPPVIHQFRYTSTRMTVGDISLPAGVHLILPTLLIHHDREVWGDDAEDFRPER 1396
            LYEVLRLYPPVI Q+++T   + +G++ +PAG+ + LPTLLIHHD E WG+DAE F+PER
Sbjct: 375  LYEVLRLYPPVIGQYQHTYAEIKIGEVLIPAGIDVTLPTLLIHHDPEYWGEDAEQFKPER 434

Query: 1397 FSEGISKASKDQVAFFPFGWGPRLCVGQNFAMIEAKMALAMILQHFEFELSSTYVHAPCT 1576
            F+ G+SKASKDQ+AFFPFGWGPR C+GQNFAM+EAK+ALAMILQ+F F+LS +Y HAP T
Sbjct: 435  FASGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQNFSFQLSPSYAHAPHT 494

Query: 1577 VITLQPQHGAQIILHHL 1627
            V+TLQPQHGAQ+ILH L
Sbjct: 495  VMTLQPQHGAQLILHQL 511


>ref|XP_003633108.1| PREDICTED: LOW QUALITY PROTEIN: secologanin synthase-like [Vitis
            vinifera]
          Length = 520

 Score =  625 bits (1613), Expect = e-177
 Identities = 301/499 (60%), Positives = 385/499 (77%), Gaps = 1/499 (0%)
 Frame = +2

Query: 134  VKVFHSLWLKPKSLEKQLKQQGIKGSPYKFLNGNLRDYTGMFKEALSKPLDLTHSIVPRV 313
            ++V +S+W KPK LEK+L+QQGI+G+PYK + G++++Y  +  EA SKP++L H IV RV
Sbjct: 22   LRVSYSIWWKPKWLEKRLRQQGIRGTPYKLVMGDMKEYIRLITEAWSKPMNLNHHIVSRV 81

Query: 314  IPYFNQLVQTHGKISLFWLGTTPTLIIKDPELMKDVLSDKSWRFQKPPENPHIKLLTMGL 493
             P+    +Q +GK+SLFW GTTP LI+ DP ++K+VLS+K   FQKP  +P I  L  GL
Sbjct: 82   DPFTQNNMQQYGKVSLFWAGTTPRLIVMDPGMIKEVLSNKQGHFQKPYISPLILTLARGL 141

Query: 494  STQEGEKWVKHRKIVMPAFHLEKLKGMMPAFSISCSTLIEKWKKSVSPQGSLELDVWPDI 673
            +  EGE W K R+I+ PAFHLEKLK M+PAF+ SCS LIE+WK+  S Q + E+D+WP++
Sbjct: 142  TALEGEVWAKXRRIINPAFHLEKLKVMIPAFTTSCSMLIERWKELASLQETCEVDIWPEL 201

Query: 674  QTFTSDVISRTAFGSSYEEGKKIFELQKEQAVLVLEAFESVYIPGFRFLPTKKNKRRMNL 853
            Q  T DVISR A GSS+EEG++IFELQKE   L LEA +++YIPGFRF+PTKKN+RR  L
Sbjct: 202  QNLTRDVISRAALGSSFEEGRQIFELQKEHITLTLEAMQTLYIPGFRFIPTKKNQRRKYL 261

Query: 854  DKEIKANLRGLIERKMLAMRNGESGDDDLIGMLLQSNNTNYLQADD-GPKVTGLTINEVI 1030
             K   +  R LI+RK  A+R G++  D+L+G+LL S++ N L  +    K   +T+ EVI
Sbjct: 262  QKRTTSMFRDLIQRKKDAIRTGQAEGDNLLGLLLLSSSQNNLPENVMSTKDNAITLEEVI 321

Query: 1031 EECKLFYFAGQETSSTWLTWTMIVLSMHPSWQEKAREEVLQVCGKNMPRFENLNHFKIVT 1210
            EECK FY AG ET+S+WLTWT+ VL+MHP+WQEKAREEV+Q+CGK  P  E L+H KIV+
Sbjct: 322  EECKQFYLAGHETTSSWLTWTVTVLAMHPNWQEKAREEVMQICGKKEPDSEALSHLKIVS 381

Query: 1211 MILYEVLRLYPPVIHQFRYTSTRMTVGDISLPAGVHLILPTLLIHHDREVWGDDAEDFRP 1390
            MILYEVLRLYPPVI  +++      +G ISLPAGV L LPTLLIHHD E+WGDDAE+F+P
Sbjct: 382  MILYEVLRLYPPVIAVYQHAYKETKIGTISLPAGVDLTLPTLLIHHDPELWGDDAEEFKP 441

Query: 1391 ERFSEGISKASKDQVAFFPFGWGPRLCVGQNFAMIEAKMALAMILQHFEFELSSTYVHAP 1570
            ERF+EG+SKASKDQ+AFFPFGWGPR C+GQNFAMIEAK+ALAMILQHF FELS +Y HAP
Sbjct: 442  ERFAEGVSKASKDQLAFFPFGWGPRTCIGQNFAMIEAKVALAMILQHFSFELSPSYTHAP 501

Query: 1571 CTVITLQPQHGAQIILHHL 1627
             TV+TLQPQHGAQ+  + L
Sbjct: 502  HTVMTLQPQHGAQLKFYQL 520


>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  625 bits (1613), Expect = e-177
 Identities = 304/499 (60%), Positives = 387/499 (77%)
 Frame = +2

Query: 131  AVKVFHSLWLKPKSLEKQLKQQGIKGSPYKFLNGNLRDYTGMFKEALSKPLDLTHSIVPR 310
            A K F  +WL P+ LEK L++QGIKG  YKFL+GNL+D     +EA +KP++L+H IVPR
Sbjct: 22   AWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDIVSATQEARAKPMELSHKIVPR 81

Query: 311  VIPYFNQLVQTHGKISLFWLGTTPTLIIKDPELMKDVLSDKSWRFQKPPENPHIKLLTMG 490
            V+P+ +Q V+ +GK+S  W G    + I DP++++D+LS+K   F+K   NP  KLL  G
Sbjct: 82   VMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILSNKFGHFEKIRTNPLGKLLISG 141

Query: 491  LSTQEGEKWVKHRKIVMPAFHLEKLKGMMPAFSISCSTLIEKWKKSVSPQGSLELDVWPD 670
            + + EGEKWVKHR+I+ PAFHLEKLK M+PA   SCS ++ KW+  VS Q S ELDVWP 
Sbjct: 142  VVSYEGEKWVKHRRILNPAFHLEKLKMMLPAIYTSCSEMVNKWEMMVS-QDSCELDVWPH 200

Query: 671  IQTFTSDVISRTAFGSSYEEGKKIFELQKEQAVLVLEAFESVYIPGFRFLPTKKNKRRMN 850
            +Q  T+DVISRTAFGSSYEEG++IF+LQ EQA LV++A +S+YIPG RFLPTK+N R   
Sbjct: 201  LQNLTADVISRTAFGSSYEEGRRIFQLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKE 260

Query: 851  LDKEIKANLRGLIERKMLAMRNGESGDDDLIGMLLQSNNTNYLQADDGPKVTGLTINEVI 1030
            +  E +  LR ++ ++  AM+ GE+ +DDL+G+L++SN    +  +   K  G+TI+EVI
Sbjct: 261  IYGEARVLLRDIVNKREKAMKIGEAHNDDLLGLLMESNFKE-ITDNVNSKNFGMTIDEVI 319

Query: 1031 EECKLFYFAGQETSSTWLTWTMIVLSMHPSWQEKAREEVLQVCGKNMPRFENLNHFKIVT 1210
            +ECKLFYFAGQET+ST L WTM+VLSMHP WQEKAREEVLQV G   P F+ LNH KIVT
Sbjct: 320  DECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVT 379

Query: 1211 MILYEVLRLYPPVIHQFRYTSTRMTVGDISLPAGVHLILPTLLIHHDREVWGDDAEDFRP 1390
            MILYEVLRLYPP +   R T   M +G+I+LPAGVHL +PTLL+HHDRE+WG+DAEDF P
Sbjct: 380  MILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNP 439

Query: 1391 ERFSEGISKASKDQVAFFPFGWGPRLCVGQNFAMIEAKMALAMILQHFEFELSSTYVHAP 1570
            ERFS G+SKA+K+QV+FFPF WGPR+C+GQNFA++E+KMA+AMILQ F FELSSTYVHAP
Sbjct: 440  ERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFELSSTYVHAP 499

Query: 1571 CTVITLQPQHGAQIILHHL 1627
             TVITLQPQHGAQ+ILH L
Sbjct: 500  YTVITLQPQHGAQLILHKL 518