BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00003324
         (950 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI34715.3| unnamed protein product [Vitis vinifera]              304   2e-80
emb|CBI34714.3| unnamed protein product [Vitis vinifera]              304   2e-80
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   304   2e-80
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   304   2e-80
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              298   1e-78

>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
          Length = 1076

 Score =  304 bits (778), Expect = 2e-80
 Identities = 150/279 (53%), Positives = 209/279 (74%), Gaps = 2/279 (0%)
 Frame = +1

Query: 118  IKSPSNLPPGPFPWPIIGNILSMGSKPHISLTKLAKTHGPLVSLRLGTQLLVVGSTPAAA 297
            +KSP  LPPGP PWPI+GN+L +G+ PHISL + ++++GPL+SLRLG+Q+LVV ST +AA
Sbjct: 603  LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 661

Query: 298  TEILKTHDRILSGRYVPHAMPESPDRFH-LSLGFT-DCTEQWKYLRTICRTDLFSAKVIE 471
             EILKTHDR+LSGRYVPHA+P      + +SLG+  +C   WK LRT+CR +LFS KV+E
Sbjct: 662  MEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVME 721

Query: 472  SQAKIREKKVTELVDFLHTKEGRMVKIGDVVFNTIFNTLGNILLSKDFADMHKESEGEAL 651
            SQA + EKKV E+V F+ TKEG ++K+G+VVF T+ NTL  +L+S+DF     +++   +
Sbjct: 722  SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGM 781

Query: 652  KGLVMKYAELVSTPNLADYYTILSGLDLQGLNKKSNEIFVQIFGIWEPIIKERREEIGSE 831
            KGLV K    ++ PNL D+Y I SGLDLQGLNKK+ E+  +I  +WE +I+ERRE    +
Sbjct: 782  KGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 841

Query: 832  VSRKRDFLDVLLDRRFTNEQINFLFLELLFAASDTSTST 948
             S K+DFL++L+   ++++QIN LF+ELL A +DTS+ST
Sbjct: 842  PS-KQDFLNILIRSGYSDDQINQLFMELLTAGADTSSST 879


>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
          Length = 936

 Score =  304 bits (778), Expect = 2e-80
 Identities = 150/279 (53%), Positives = 209/279 (74%), Gaps = 2/279 (0%)
 Frame = +1

Query: 118  IKSPSNLPPGPFPWPIIGNILSMGSKPHISLTKLAKTHGPLVSLRLGTQLLVVGSTPAAA 297
            +KSP  LPPGP PWPI+GN+L +G+ PHISL + ++++GPL+SLRLG+Q+LVV ST +AA
Sbjct: 472  LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 530

Query: 298  TEILKTHDRILSGRYVPHAMPESPDRFH-LSLGFT-DCTEQWKYLRTICRTDLFSAKVIE 471
             EILKTHDR+LSGRYVPHA+P      + +SLG+  +C + WK LRT+CR +LFS KV+E
Sbjct: 531  MEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVME 590

Query: 472  SQAKIREKKVTELVDFLHTKEGRMVKIGDVVFNTIFNTLGNILLSKDFADMHKESEGEAL 651
            SQA + EKKV E+V F+ TKEG ++K+G+VVF T+ NTL  +L+S+DF     + +   +
Sbjct: 591  SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGM 650

Query: 652  KGLVMKYAELVSTPNLADYYTILSGLDLQGLNKKSNEIFVQIFGIWEPIIKERREEIGSE 831
            KGLV K    ++ PNL D+Y I SGLDLQGLNKK+ E+  +I  +WE +I+ERRE    +
Sbjct: 651  KGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 710

Query: 832  VSRKRDFLDVLLDRRFTNEQINFLFLELLFAASDTSTST 948
             S K+DFL++L+   ++++QIN LF+ELL A +DTS+ST
Sbjct: 711  PS-KQDFLNILIRSGYSDDQINQLFMELLTAGADTSSST 748


>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
           2-like [Vitis vinifera]
          Length = 503

 Score =  304 bits (778), Expect = 2e-80
 Identities = 150/279 (53%), Positives = 209/279 (74%), Gaps = 2/279 (0%)
 Frame = +1

Query: 118 IKSPSNLPPGPFPWPIIGNILSMGSKPHISLTKLAKTHGPLVSLRLGTQLLVVGSTPAAA 297
           +KSP  LPPGP PWPI+GN+L +G+ PHISL + ++++GPL+SLRLG+Q+LVV ST +AA
Sbjct: 39  LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 97

Query: 298 TEILKTHDRILSGRYVPHAMPESPDRFH-LSLGFT-DCTEQWKYLRTICRTDLFSAKVIE 471
            EILKTHDR+LSGRYVPHA+P      + +SLG+  +C + WK LRT+CR +LFS KV+E
Sbjct: 98  MEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVME 157

Query: 472 SQAKIREKKVTELVDFLHTKEGRMVKIGDVVFNTIFNTLGNILLSKDFADMHKESEGEAL 651
           SQA + EKKV E+V F+ TKEG ++K+G+VVF T+ NTL  +L+S+DF     + +   +
Sbjct: 158 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGM 217

Query: 652 KGLVMKYAELVSTPNLADYYTILSGLDLQGLNKKSNEIFVQIFGIWEPIIKERREEIGSE 831
           KGLV K    ++ PNL D+Y I SGLDLQGLNKK+ E+  +I  +WE +I+ERRE    +
Sbjct: 218 KGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 277

Query: 832 VSRKRDFLDVLLDRRFTNEQINFLFLELLFAASDTSTST 948
            S K+DFL++L+   ++++QIN LF+ELL A +DTS+ST
Sbjct: 278 PS-KQDFLNILIRSGYSDDQINQLFMELLTAGADTSSST 315


>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
           [Vitis vinifera]
          Length = 503

 Score =  304 bits (778), Expect = 2e-80
 Identities = 150/279 (53%), Positives = 209/279 (74%), Gaps = 2/279 (0%)
 Frame = +1

Query: 118 IKSPSNLPPGPFPWPIIGNILSMGSKPHISLTKLAKTHGPLVSLRLGTQLLVVGSTPAAA 297
           +KSP  LPPGP PWPI+GN+L +G+ PHISL + ++++GPL+SLRLG+Q+LVV ST +AA
Sbjct: 39  LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 97

Query: 298 TEILKTHDRILSGRYVPHAMPESPDRFH-LSLGFT-DCTEQWKYLRTICRTDLFSAKVIE 471
            EILKTHDR+LSGRYVPHA+P      + +SLG+  +C   WK LRT+CR +LFS KV+E
Sbjct: 98  MEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVME 157

Query: 472 SQAKIREKKVTELVDFLHTKEGRMVKIGDVVFNTIFNTLGNILLSKDFADMHKESEGEAL 651
           SQA + EKKV E+V F+ TKEG ++K+G+VVF T+ NTL  +L+S+DF     +++   +
Sbjct: 158 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGM 217

Query: 652 KGLVMKYAELVSTPNLADYYTILSGLDLQGLNKKSNEIFVQIFGIWEPIIKERREEIGSE 831
           KGLV K    ++ PNL D+Y I SGLDLQGLNKK+ E+  +I  +WE +I+ERRE    +
Sbjct: 218 KGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 277

Query: 832 VSRKRDFLDVLLDRRFTNEQINFLFLELLFAASDTSTST 948
            S K+DFL++L+   ++++QIN LF+ELL A +DTS+ST
Sbjct: 278 PS-KQDFLNILIRSGYSDDQINQLFMELLTAGADTSSST 315


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  298 bits (764), Expect = 1e-78
 Identities = 159/283 (56%), Positives = 199/283 (70%), Gaps = 4/283 (1%)
 Frame = +1

Query: 112  KHI-KSPSNLPPGPFPWPIIGNILSMGSKPHISLTKLAKTHGPLVSLRLGTQLLVVGSTP 288
            KHI   P  LPPGP+PWPIIGN+L MG  PH  L  LAK HGPL+SLRLGTQL+VV S+P
Sbjct: 569  KHIFLKPPPLPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSP 628

Query: 289  AAATEILKTHDRILSGRYVPHAMP-ESPDRFHLSLGFT-DCTEQWKYLRTICRTDLFSAK 462
            AAA E+LKTHDR LSGRY+  ++P ++P   HLS+ F  DC   WK LR ICR +LFS K
Sbjct: 629  AAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGK 688

Query: 463  VIESQAKIREKKVTELVDFLHTKEGRMVKIGDVVFNTIFNTLGNILLSKDFADMHKESE- 639
             +ESQ ++RE+KVTELV+FL TKEG +VK+ D+VF TI N L N   S D  D   E   
Sbjct: 689  AMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRV 748

Query: 640  GEALKGLVMKYAELVSTPNLADYYTILSGLDLQGLNKKSNEIFVQIFGIWEPIIKERREE 819
            G ALK L+ K AE  +TPNL+DYY IL GLD+QG+N+K+ E+F +I   WE I+KERR +
Sbjct: 749  GGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQ 808

Query: 820  IGSEVSRKRDFLDVLLDRRFTNEQINFLFLELLFAASDTSTST 948
              S  S  RDFL+ LL+  F ++QIN + LEL  A ++TS+ T
Sbjct: 809  -RSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLT 850



 Score =  288 bits (736), Expect = 2e-75
 Identities = 153/282 (54%), Positives = 192/282 (68%), Gaps = 3/282 (1%)
 Frame = +1

Query: 112  KHI-KSPSNLPPGPFPWPIIGNILSMGSKPHISLTKLAKTHGPLVSLRLGTQLLVVGSTP 288
            KHI   P  LPPGP+PWPIIGN+L +G  PH+ L  LAK HGPL+SLRLGTQL+VV S+P
Sbjct: 1074 KHIFLKPPPLPPGPYPWPIIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSP 1133

Query: 289  AAATEILKTHDRILSGRYVPHAMP-ESPDRFHLSLGFT-DCTEQWKYLRTICRTDLFSAK 462
            AAA E+LKTHDR LSGRYV  ++  + P   HLSL F  +CT  WK LRTICRT++FS K
Sbjct: 1134 AAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGK 1193

Query: 463  VIESQAKIREKKVTELVDFLHTKEGRMVKIGDVVFNTIFNTLGNILLSKDFADMHKESEG 642
             +ES  ++RE+KV ELV+FL TKEG +VK+ D+VF TI N L N   S D  D     E 
Sbjct: 1194 AMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDF----ER 1249

Query: 643  EALKGLVMKYAELVSTPNLADYYTILSGLDLQGLNKKSNEIFVQIFGIWEPIIKERREEI 822
            E LK  + + AEL +TPNL+D+Y IL GL+L G  KKS E   +I   WE  +KERR++ 
Sbjct: 1250 EGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQ- 1308

Query: 823  GSEVSRKRDFLDVLLDRRFTNEQINFLFLELLFAASDTSTST 948
             +  S  RD L+  L+ RF ++QIN + LEL  A +DTST T
Sbjct: 1309 KNPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLT 1350



 Score =  269 bits (687), Expect = 8e-70
 Identities = 147/284 (51%), Positives = 193/284 (67%), Gaps = 5/284 (1%)
 Frame = +1

Query: 112 KHI--KSPSNLPPGPFPWPIIGNILSMGSKPHISLTKLAKTHGPLVSLRLGTQLLVVGST 285
           KHI  KSP  LPPGP+PWPIIGN+L MG   H+ L  LAK HGPL+SLRLGTQ++VV S+
Sbjct: 29  KHIFLKSPP-LPPGPYPWPIIGNLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASS 87

Query: 286 PAAATEILKTHDRILSGRYVPHAMP-ESPDRFHLSLGFTD-CTEQWKYLRTICRTDLFSA 459
            AAA E+LKTHDR LSGRYV   +P  SP   HL++ F   C   W+ L+ ICR +LFS 
Sbjct: 88  SAAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSG 147

Query: 460 KVIESQAKIREKKVTELVDFLHTKEGRMVKIGDVVFNTIFNTLGNILLSKDFADMH-KES 636
           K +ES+ ++RE+KV ELV+FL  KEG +VK+ D+V+ T+ N L N   S DF+D   ++ 
Sbjct: 148 KAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDV 207

Query: 637 EGEALKGLVMKYAELVSTPNLADYYTILSGLDLQGLNKKSNEIFVQIFGIWEPIIKERRE 816
            G  LK L+ + AEL +T N+ D+Y IL GLD+QG+ KK  EIF +I   WE I+KERR+
Sbjct: 208 RGVLLKDLLNENAELGAT-NILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRK 266

Query: 817 EIGSEVSRKRDFLDVLLDRRFTNEQINFLFLELLFAASDTSTST 948
           +     S   DFLD LL+  F ++QIN + +EL FA  +TS+ T
Sbjct: 267 Q-RIHGSSHGDFLDALLETGFEDDQINHVIMELFFAGPETSSLT 309