BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00003254
         (2167 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003631773.1| PREDICTED: NADP-dependent malic enzyme-like ...   978   0.0  
emb|CBI20568.3| unnamed protein product [Vitis vinifera]              978   0.0  
gb|AAB08874.1| malate dehydrogenase [Vitis vinifera]                  974   0.0  
ref|XP_002283814.1| PREDICTED: NADP-dependent malic enzyme [Viti...   974   0.0  
dbj|BAA24950.1| NADP-malic enzyme [Aloe arborescens]                  966   0.0  

>ref|XP_003631773.1| PREDICTED: NADP-dependent malic enzyme-like [Vitis vinifera]
          Length = 585

 Score =  978 bits (2528), Expect = 0.0
 Identities = 477/549 (86%), Positives = 525/549 (95%)
 Frame = +1

Query: 286  TPWTVSVASGYSLLRSPFHNKGLAFSEKERDAHYLRGLLPPTVISQDLQVKKMMHSLRKY 465
            TPWTVSVASGY+LLR P HNKGLAF+E ERDAHYLRGLLPP V++QDLQ K++MH+LR+Y
Sbjct: 37   TPWTVSVASGYTLLRDPHHNKGLAFTENERDAHYLRGLLPPAVLNQDLQEKRLMHNLRQY 96

Query: 466  EIPLQRYVAMMDLQETNERLFYKLLMDNVEELLPVVYTPTVGEACQKYGSIARRPQGLYI 645
            ++PLQRY+AMMDLQE NERLFYKLL+DNVEELLPVVYTPTVGEACQKYGSI RRPQGLYI
Sbjct: 97   KVPLQRYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYI 156

Query: 646  SLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGLRP 825
            SLKEKGKILEVLKNWPER+IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGLRP
Sbjct: 157  SLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGLRP 216

Query: 826  SSCLPITIDVGTNNEKLLKDDYYIGLRQRRATGQEYDELLHEFMCAVKQMYGEKVLIQFE 1005
            S+CLP+TIDVGTNNE+LLKD++YIGL+QRRATGQEY ELLHEFMCAVKQ YGE+VLIQFE
Sbjct: 217  SACLPVTIDVGTNNEQLLKDEFYIGLKQRRATGQEYAELLHEFMCAVKQNYGERVLIQFE 276

Query: 1006 DFANHNAFELLSKYRTSHLVFNDDIQGTASVVLSGLFAALKLVGGTLTEQTFLFLGAGEA 1185
            DFANHNAFELL+KY  +HLVFNDDIQGTASVVL+GL A+LKLVGG+L E TFLFLGAGEA
Sbjct: 277  DFANHNAFELLAKYSKTHLVFNDDIQGTASVVLAGLIASLKLVGGSLAEHTFLFLGAGEA 336

Query: 1186 GTGIAELIALEMSKQTEAPLEETRKKIWLVDSKGLIVNSRKESLQHFKKPWAHDHEPINN 1365
            GTGIAELIALE+SKQT+APLEETRKKIWLVDSKGLIV+SR ESLQHFKKPWAHDHEPI  
Sbjct: 337  GTGIAELIALEISKQTKAPLEETRKKIWLVDSKGLIVHSRMESLQHFKKPWAHDHEPIRA 396

Query: 1366 LLDAVKAIKPTILIGSSGVGQTFTKEVVESMTSFNEKPIIFALSNPTSQSECTAEEAYTW 1545
            LLDAVK+IKPT+LIGSSGVG+TFTKEVVE+MTSFN+KP+I ALSNPTSQSECTAEEAYTW
Sbjct: 397  LLDAVKSIKPTVLIGSSGVGKTFTKEVVEAMTSFNKKPLILALSNPTSQSECTAEEAYTW 456

Query: 1546 SKGRAIFASGSPFNPVEYEGKVYVPGQSNNAYIFPGFGLGLIISGAIRVHDDMLLAASEA 1725
            SKGRAIFASGSPF+PVEY GK++VPGQ+NNAYIFPGFGLGLI+SGAIRVHDDMLLAASEA
Sbjct: 457  SKGRAIFASGSPFDPVEYNGKLFVPGQANNAYIFPGFGLGLIMSGAIRVHDDMLLAASEA 516

Query: 1726 LAAQVTEENFSKGMLFPRFSNIRKISAHIAANVAAKAYELGLASRLPRPDNLVEYAESCM 1905
            LAAQVT+EN+ KG+++P F NIRKISAHIAANVAAKAY+LGLASRLPRP++LV+YAESCM
Sbjct: 517  LAAQVTQENYDKGLIYPPFCNIRKISAHIAANVAAKAYQLGLASRLPRPEHLVKYAESCM 576

Query: 1906 YSPTYHSYR 1932
            YSP Y SYR
Sbjct: 577  YSPVYRSYR 585


>emb|CBI20568.3| unnamed protein product [Vitis vinifera]
          Length = 575

 Score =  978 bits (2528), Expect = 0.0
 Identities = 477/549 (86%), Positives = 525/549 (95%)
 Frame = +1

Query: 286  TPWTVSVASGYSLLRSPFHNKGLAFSEKERDAHYLRGLLPPTVISQDLQVKKMMHSLRKY 465
            TPWTVSVASGY+LLR P HNKGLAF+E ERDAHYLRGLLPP V++QDLQ K++MH+LR+Y
Sbjct: 27   TPWTVSVASGYTLLRDPHHNKGLAFTENERDAHYLRGLLPPAVLNQDLQEKRLMHNLRQY 86

Query: 466  EIPLQRYVAMMDLQETNERLFYKLLMDNVEELLPVVYTPTVGEACQKYGSIARRPQGLYI 645
            ++PLQRY+AMMDLQE NERLFYKLL+DNVEELLPVVYTPTVGEACQKYGSI RRPQGLYI
Sbjct: 87   KVPLQRYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYI 146

Query: 646  SLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGLRP 825
            SLKEKGKILEVLKNWPER+IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGLRP
Sbjct: 147  SLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGLRP 206

Query: 826  SSCLPITIDVGTNNEKLLKDDYYIGLRQRRATGQEYDELLHEFMCAVKQMYGEKVLIQFE 1005
            S+CLP+TIDVGTNNE+LLKD++YIGL+QRRATGQEY ELLHEFMCAVKQ YGE+VLIQFE
Sbjct: 207  SACLPVTIDVGTNNEQLLKDEFYIGLKQRRATGQEYAELLHEFMCAVKQNYGERVLIQFE 266

Query: 1006 DFANHNAFELLSKYRTSHLVFNDDIQGTASVVLSGLFAALKLVGGTLTEQTFLFLGAGEA 1185
            DFANHNAFELL+KY  +HLVFNDDIQGTASVVL+GL A+LKLVGG+L E TFLFLGAGEA
Sbjct: 267  DFANHNAFELLAKYSKTHLVFNDDIQGTASVVLAGLIASLKLVGGSLAEHTFLFLGAGEA 326

Query: 1186 GTGIAELIALEMSKQTEAPLEETRKKIWLVDSKGLIVNSRKESLQHFKKPWAHDHEPINN 1365
            GTGIAELIALE+SKQT+APLEETRKKIWLVDSKGLIV+SR ESLQHFKKPWAHDHEPI  
Sbjct: 327  GTGIAELIALEISKQTKAPLEETRKKIWLVDSKGLIVHSRMESLQHFKKPWAHDHEPIRA 386

Query: 1366 LLDAVKAIKPTILIGSSGVGQTFTKEVVESMTSFNEKPIIFALSNPTSQSECTAEEAYTW 1545
            LLDAVK+IKPT+LIGSSGVG+TFTKEVVE+MTSFN+KP+I ALSNPTSQSECTAEEAYTW
Sbjct: 387  LLDAVKSIKPTVLIGSSGVGKTFTKEVVEAMTSFNKKPLILALSNPTSQSECTAEEAYTW 446

Query: 1546 SKGRAIFASGSPFNPVEYEGKVYVPGQSNNAYIFPGFGLGLIISGAIRVHDDMLLAASEA 1725
            SKGRAIFASGSPF+PVEY GK++VPGQ+NNAYIFPGFGLGLI+SGAIRVHDDMLLAASEA
Sbjct: 447  SKGRAIFASGSPFDPVEYNGKLFVPGQANNAYIFPGFGLGLIMSGAIRVHDDMLLAASEA 506

Query: 1726 LAAQVTEENFSKGMLFPRFSNIRKISAHIAANVAAKAYELGLASRLPRPDNLVEYAESCM 1905
            LAAQVT+EN+ KG+++P F NIRKISAHIAANVAAKAY+LGLASRLPRP++LV+YAESCM
Sbjct: 507  LAAQVTQENYDKGLIYPPFCNIRKISAHIAANVAAKAYQLGLASRLPRPEHLVKYAESCM 566

Query: 1906 YSPTYHSYR 1932
            YSP Y SYR
Sbjct: 567  YSPVYRSYR 575


>gb|AAB08874.1| malate dehydrogenase [Vitis vinifera]
          Length = 640

 Score =  974 bits (2518), Expect = 0.0
 Identities = 487/617 (78%), Positives = 538/617 (87%), Gaps = 26/617 (4%)
 Frame = +1

Query: 160  QRKASAKVVVALSSNGRGVEKKI------------------EXXXXXXXXXXXXXXXMED 285
            QRK    VV+AL SNGR  E  +                  E                ED
Sbjct: 24   QRKVLPPVVLALGSNGRAGELNVSAVMESTLKELRDGNTVLEVDSKSAVSGGVRDVQGED 83

Query: 286  --------TPWTVSVASGYSLLRSPFHNKGLAFSEKERDAHYLRGLLPPTVISQDLQVKK 441
                    TPWT++VASGYSLLR+P HNKGLAF+EKERD HYLRGLLPP V+SQDLQVKK
Sbjct: 84   AATEDQIVTPWTITVASGYSLLRNPHHNKGLAFTEKERDHHYLRGLLPPAVVSQDLQVKK 143

Query: 442  MMHSLRKYEIPLQRYVAMMDLQETNERLFYKLLMDNVEELLPVVYTPTVGEACQKYGSIA 621
            +M ++R+Y +PLQ+Y+AMMDLQE NE+LFYKLLMDNVEELLPVVYTPTVGEACQKYG I 
Sbjct: 144  LMANIRQYTVPLQKYMAMMDLQERNEKLFYKLLMDNVEELLPVVYTPTVGEACQKYGGIL 203

Query: 622  RRPQGLYISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 801
            RRPQGL+ISL EKGKILEVLKNWPE+NIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY
Sbjct: 204  RRPQGLFISLNEKGKILEVLKNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 263

Query: 802  TALGGLRPSSCLPITIDVGTNNEKLLKDDYYIGLRQRRATGQEYDELLHEFMCAVKQMYG 981
            TALGG+RPS+CLP+TIDVGTNN+KLL D++YIGLRQ+RATGQEY EL+HEFMCAVKQ YG
Sbjct: 264  TALGGVRPSACLPVTIDVGTNNQKLLDDEFYIGLRQKRATGQEYAELIHEFMCAVKQNYG 323

Query: 982  EKVLIQFEDFANHNAFELLSKYRTSHLVFNDDIQGTASVVLSGLFAALKLVGGTLTEQTF 1161
            EKVL+QFEDFANHNAF+LL++Y T+HLVFNDDIQGTASVVL+GL +AL LVGGTL E TF
Sbjct: 324  EKVLVQFEDFANHNAFDLLARYGTTHLVFNDDIQGTASVVLAGLISALNLVGGTLAEHTF 383

Query: 1162 LFLGAGEAGTGIAELIALEMSKQTEAPLEETRKKIWLVDSKGLIVNSRKESLQHFKKPWA 1341
            LFLGAGEAGTGIAELIALEMSKQT+APLEETRKKIWLVDSKGLIV+SR +SLQHFKKPWA
Sbjct: 384  LFLGAGEAGTGIAELIALEMSKQTQAPLEETRKKIWLVDSKGLIVSSRMDSLQHFKKPWA 443

Query: 1342 HDHEPINNLLDAVKAIKPTILIGSSGVGQTFTKEVVESMTSFNEKPIIFALSNPTSQSEC 1521
            H+HEPI NL+DAVKAIKPT+LIGSSGVG+TFTKEVVE+M SFNEKPII ALSNPTSQSEC
Sbjct: 444  HEHEPIKNLVDAVKAIKPTVLIGSSGVGRTFTKEVVEAMASFNEKPIILALSNPTSQSEC 503

Query: 1522 TAEEAYTWSKGRAIFASGSPFNPVEYEGKVYVPGQSNNAYIFPGFGLGLIISGAIRVHDD 1701
            TAEEAYTWS+GRAIFASGSPF+PVEY GKV+VPGQSNNAYIFPGFGLGLIISGAIRVHDD
Sbjct: 504  TAEEAYTWSEGRAIFASGSPFDPVEYNGKVFVPGQSNNAYIFPGFGLGLIISGAIRVHDD 563

Query: 1702 MLLAASEALAAQVTEENFSKGMLFPRFSNIRKISAHIAANVAAKAYELGLASRLPRPDNL 1881
            MLLAASEALA Q T+ENF KGM++P FSNIRKISAHIAANVAAKAYELGLA+RLP+P+NL
Sbjct: 564  MLLAASEALAKQATQENFDKGMIYPPFSNIRKISAHIAANVAAKAYELGLATRLPQPENL 623

Query: 1882 VEYAESCMYSPTYHSYR 1932
            VEYAE+CMYSP Y S+R
Sbjct: 624  VEYAENCMYSPAYRSFR 640


>ref|XP_002283814.1| PREDICTED: NADP-dependent malic enzyme [Vitis vinifera]
            gi|297743201|emb|CBI36068.3| unnamed protein product
            [Vitis vinifera]
          Length = 640

 Score =  974 bits (2518), Expect = 0.0
 Identities = 487/617 (78%), Positives = 538/617 (87%), Gaps = 26/617 (4%)
 Frame = +1

Query: 160  QRKASAKVVVALSSNGRGVEKKI------------------EXXXXXXXXXXXXXXXMED 285
            QRK    VV+AL SNGR  E  +                  E                ED
Sbjct: 24   QRKVLPPVVLALGSNGRAGELNVSAVMESTLKELRDGNTVLEVDSKSAVSGGVRDVQGED 83

Query: 286  --------TPWTVSVASGYSLLRSPFHNKGLAFSEKERDAHYLRGLLPPTVISQDLQVKK 441
                    TPWT++VASGYSLLR+P HNKGLAF+EKERD HYLRGLLPP V+SQDLQVKK
Sbjct: 84   AATEDQIVTPWTITVASGYSLLRNPHHNKGLAFTEKERDHHYLRGLLPPAVVSQDLQVKK 143

Query: 442  MMHSLRKYEIPLQRYVAMMDLQETNERLFYKLLMDNVEELLPVVYTPTVGEACQKYGSIA 621
            +M ++R+Y +PLQ+Y+AMMDLQE NE+LFYKLLMDNVEELLPVVYTPTVGEACQKYG I 
Sbjct: 144  LMANIRQYTVPLQKYMAMMDLQERNEKLFYKLLMDNVEELLPVVYTPTVGEACQKYGGIL 203

Query: 622  RRPQGLYISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 801
            RRPQGL+ISL EKGKILEVLKNWPE+NIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY
Sbjct: 204  RRPQGLFISLNEKGKILEVLKNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 263

Query: 802  TALGGLRPSSCLPITIDVGTNNEKLLKDDYYIGLRQRRATGQEYDELLHEFMCAVKQMYG 981
            TALGG+RPS+CLP+TIDVGTNN+KLL D++YIGLRQ+RATGQEY EL+HEFMCAVKQ YG
Sbjct: 264  TALGGVRPSACLPVTIDVGTNNQKLLDDEFYIGLRQKRATGQEYAELIHEFMCAVKQNYG 323

Query: 982  EKVLIQFEDFANHNAFELLSKYRTSHLVFNDDIQGTASVVLSGLFAALKLVGGTLTEQTF 1161
            EKVL+QFEDFANHNAF+LL++Y T+HLVFNDDIQGTASVVL+GL +AL LVGGTL E TF
Sbjct: 324  EKVLVQFEDFANHNAFDLLARYGTTHLVFNDDIQGTASVVLAGLISALNLVGGTLAEHTF 383

Query: 1162 LFLGAGEAGTGIAELIALEMSKQTEAPLEETRKKIWLVDSKGLIVNSRKESLQHFKKPWA 1341
            LFLGAGEAGTGIAELIALEMSKQT+APLEETRKKIWLVDSKGLIV+SR +SLQHFKKPWA
Sbjct: 384  LFLGAGEAGTGIAELIALEMSKQTQAPLEETRKKIWLVDSKGLIVSSRMDSLQHFKKPWA 443

Query: 1342 HDHEPINNLLDAVKAIKPTILIGSSGVGQTFTKEVVESMTSFNEKPIIFALSNPTSQSEC 1521
            H+HEPI NL+DAVKAIKPT+LIGSSGVG+TFTKEVVE+M SFNEKPII ALSNPTSQSEC
Sbjct: 444  HEHEPIKNLVDAVKAIKPTVLIGSSGVGRTFTKEVVEAMASFNEKPIILALSNPTSQSEC 503

Query: 1522 TAEEAYTWSKGRAIFASGSPFNPVEYEGKVYVPGQSNNAYIFPGFGLGLIISGAIRVHDD 1701
            TAEEAYTWS+GRAIFASGSPF+PVEY GKV+VPGQSNNAYIFPGFGLGLIISGAIRVHDD
Sbjct: 504  TAEEAYTWSEGRAIFASGSPFDPVEYNGKVFVPGQSNNAYIFPGFGLGLIISGAIRVHDD 563

Query: 1702 MLLAASEALAAQVTEENFSKGMLFPRFSNIRKISAHIAANVAAKAYELGLASRLPRPDNL 1881
            MLLAASEALA Q T+ENF KGM++P FSNIRKISAHIAANVAAKAYELGLA+RLP+P+NL
Sbjct: 564  MLLAASEALAKQATQENFDKGMIYPPFSNIRKISAHIAANVAAKAYELGLATRLPQPENL 623

Query: 1882 VEYAESCMYSPTYHSYR 1932
            VEYAE+CMYSP Y S+R
Sbjct: 624  VEYAENCMYSPVYRSFR 640


>dbj|BAA24950.1| NADP-malic enzyme [Aloe arborescens]
          Length = 592

 Score =  966 bits (2498), Expect = 0.0
 Identities = 473/549 (86%), Positives = 520/549 (94%)
 Frame = +1

Query: 286  TPWTVSVASGYSLLRSPFHNKGLAFSEKERDAHYLRGLLPPTVISQDLQVKKMMHSLRKY 465
            TPWTVSVASGYSLLR P HNKGLAFSEKERDAHYLRGLLPPT ISQ++QVKKM+H+LR+Y
Sbjct: 44   TPWTVSVASGYSLLRDPHHNKGLAFSEKERDAHYLRGLLPPTCISQEIQVKKMLHNLRQY 103

Query: 466  EIPLQRYVAMMDLQETNERLFYKLLMDNVEELLPVVYTPTVGEACQKYGSIARRPQGLYI 645
            ++PLQRY+AMMDLQE NERLFYKLL+D+VEELLPVVYTPTVGEACQKYG I RRPQGLYI
Sbjct: 104  QVPLQRYMAMMDLQEMNERLFYKLLIDHVEELLPVVYTPTVGEACQKYGCIFRRPQGLYI 163

Query: 646  SLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGLRP 825
            SLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RP
Sbjct: 164  SLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRP 223

Query: 826  SSCLPITIDVGTNNEKLLKDDYYIGLRQRRATGQEYDELLHEFMCAVKQMYGEKVLIQFE 1005
            S+CLP+TIDVGTNNE+LLKD++YIGLRQ+RATGQEY ELLHEFM AVKQ YGEKVLIQFE
Sbjct: 224  SACLPVTIDVGTNNEQLLKDEFYIGLRQKRATGQEYAELLHEFMAAVKQNYGEKVLIQFE 283

Query: 1006 DFANHNAFELLSKYRTSHLVFNDDIQGTASVVLSGLFAALKLVGGTLTEQTFLFLGAGEA 1185
            DFANHNAFELL+KY TSHLVFNDDIQGTASVVL+GL AALKLVGGTL E TFLFLGAGEA
Sbjct: 284  DFANHNAFELLAKYSTSHLVFNDDIQGTASVVLAGLVAALKLVGGTLAEHTFLFLGAGEA 343

Query: 1186 GTGIAELIALEMSKQTEAPLEETRKKIWLVDSKGLIVNSRKESLQHFKKPWAHDHEPINN 1365
            GTGIAELIALEMSKQT+AP+EETRKKIWLVDSKGLIV+SRK++LQHFKKPWAH+HEP++ 
Sbjct: 344  GTGIAELIALEMSKQTKAPVEETRKKIWLVDSKGLIVSSRKDTLQHFKKPWAHEHEPVDT 403

Query: 1366 LLDAVKAIKPTILIGSSGVGQTFTKEVVESMTSFNEKPIIFALSNPTSQSECTAEEAYTW 1545
            LL AVK IKPT+LIGSSGVG+TFTKEV+E+M+SF EKP+I ALSNPTSQSECTAEEAYTW
Sbjct: 404  LLGAVKTIKPTVLIGSSGVGRTFTKEVIEAMSSFTEKPVILALSNPTSQSECTAEEAYTW 463

Query: 1546 SKGRAIFASGSPFNPVEYEGKVYVPGQSNNAYIFPGFGLGLIISGAIRVHDDMLLAASEA 1725
            SKGRAIFASGSPF+PV Y GK++VPGQ+NNAYIFPG GLGL+ISGAIRVHDDMLLAASEA
Sbjct: 464  SKGRAIFASGSPFDPVLYNGKLFVPGQANNAYIFPGLGLGLVISGAIRVHDDMLLAASEA 523

Query: 1726 LAAQVTEENFSKGMLFPRFSNIRKISAHIAANVAAKAYELGLASRLPRPDNLVEYAESCM 1905
            LA QVT+ENF+ G+++P FS IRKISA IAANVAAKAYELGLA+RLPRP +LV+YAESCM
Sbjct: 524  LAQQVTQENFANGLIYPPFSIIRKISAQIAANVAAKAYELGLATRLPRPADLVKYAESCM 583

Query: 1906 YSPTYHSYR 1932
            Y+P Y SYR
Sbjct: 584  YTPAYRSYR 592