BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00002047
         (1828 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004139500.1| PREDICTED: bifunctional nitrilase/nitrile hy...   577   e-162
ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hy...   572   e-160
sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nit...   570   e-160
ref|XP_002328237.1| nitrilase 1 [Populus trichocarpa] gi|2228377...   570   e-160
gb|AFK41272.1| unknown [Lotus japonicus]                              569   e-159

>ref|XP_004139500.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A-like
            [Cucumis sativus]
          Length = 350

 Score =  577 bits (1486), Expect = e-162
 Identities = 280/352 (79%), Positives = 311/352 (88%), Gaps = 3/352 (0%)
 Frame = +3

Query: 261  MALVPTPPSISNNGAPVIAEVDMGSDXXXXXXXXXXXXXXXIFYDTPATLDKAERLIADA 440
            MALVPT     +    +IAEVDMGSD               IFYDTPATLDKAERL+A+A
Sbjct: 1    MALVPTTAPTDSQ---LIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEA 57

Query: 441  SLYGAQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEEFRKYHASAIDVPGPEVERLAAM 620
            + +G+QLVVFPEAF+GGYPRGSNFG++IG+RT KGKEEFRKYHASAIDVPGPEV+RLAAM
Sbjct: 58   AGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAM 117

Query: 621  AGKYKVYLVMGVIEREGYTLYCTILFFDPQGQYLGKHRKLMPTALERVIWGFGDGSTIPV 800
            AGKY+V+LVMGVIER+GYTLYCT+LFFDPQG+YLGKHRK+MPTALER+IWGFGDGSTIPV
Sbjct: 118  AGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPV 177

Query: 801  FETSIGKIGALICWENKMPLLRTAMYGKGIEIYCAPTADSRELWQASMTHIALEGGCFVL 980
            FETSIGKIGA ICWEN+MPLLRTAMY KGIEIYCAPTADSR+ WQASMTHIALEGGCFVL
Sbjct: 178  FETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVL 237

Query: 981  SANQFCRRKDYPPPPEYTFSGIDEDYSPESVVCAGGSVIISPSGTVLAGPNYEGEALISA 1160
            SANQFCRRKDYPPPPEY FSG +E+ +P+SVVCAGGS IISPSGT+LAGPNY+GEALISA
Sbjct: 238  SANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISA 297

Query: 1161 DLDFGEIVRAKFDFDVVGHYARPDVLSLIVRDHPTTPVSFTA---KTEGSQK 1307
            DLD GEI RAKFDFDVVGHYARP+VLSL+VRDHPTTPV+FT+   K E S K
Sbjct: 298  DLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 349


>ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A-like
            [Glycine max]
          Length = 350

 Score =  572 bits (1474), Expect = e-160
 Identities = 273/342 (79%), Positives = 305/342 (89%)
 Frame = +3

Query: 261  MALVPTPPSISNNGAPVIAEVDMGSDXXXXXXXXXXXXXXXIFYDTPATLDKAERLIADA 440
            MALV T P+I  N  P+ AEVDMGSD               IFYDTPATLDKAERL+A+A
Sbjct: 1    MALVTTNPTI--NEGPLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEA 58

Query: 441  SLYGAQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEEFRKYHASAIDVPGPEVERLAAM 620
            + YG+QLVVFPEAF+GGYPRGS FGL+IG+RT KG+EEFRKYH++AIDVPGPEV+RLAAM
Sbjct: 59   ASYGSQLVVFPEAFVGGYPRGSAFGLSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLAAM 118

Query: 621  AGKYKVYLVMGVIEREGYTLYCTILFFDPQGQYLGKHRKLMPTALERVIWGFGDGSTIPV 800
            AGKYKV+LVMGVIER+GYTLYCT+LFFD QG YLGKHRK+MPTALERVIWGFGDGSTIPV
Sbjct: 119  AGKYKVHLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERVIWGFGDGSTIPV 178

Query: 801  FETSIGKIGALICWENKMPLLRTAMYGKGIEIYCAPTADSRELWQASMTHIALEGGCFVL 980
            FET +GKIGA ICWEN+MPLLRTAMY KG+EIYCAPTADSR++WQASMTHIALEGGCFVL
Sbjct: 179  FETPVGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADSRDVWQASMTHIALEGGCFVL 238

Query: 981  SANQFCRRKDYPPPPEYTFSGIDEDYSPESVVCAGGSVIISPSGTVLAGPNYEGEALISA 1160
            SANQFCRRKDYPPPPEY F+G +ED +P+SVVCAGGSVIISPSG VLAGPNY+GEALISA
Sbjct: 239  SANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPSGAVLAGPNYDGEALISA 298

Query: 1161 DLDFGEIVRAKFDFDVVGHYARPDVLSLIVRDHPTTPVSFTA 1286
            DLD GEI RAKFDFDVVGHY+RP+VLSLIV+DHPT PV+FT+
Sbjct: 299  DLDLGEIARAKFDFDVVGHYSRPEVLSLIVKDHPTNPVTFTS 340


>sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4B;
            Short=TNIT4B; AltName: Full=Cyanoalanine nitrilase B;
            AltName: Full=Nitrilase 4B gi|1181615|dbj|BAA11770.1|
            nitrilase [Nicotiana tabacum]
          Length = 348

 Score =  570 bits (1470), Expect = e-160
 Identities = 278/350 (79%), Positives = 307/350 (87%)
 Frame = +3

Query: 261  MALVPTPPSISNNGAPVIAEVDMGSDXXXXXXXXXXXXXXXIFYDTPATLDKAERLIADA 440
            MALVPTP  + N G P+ AEVDMG +               IFYDTPATLDKAERL+A+A
Sbjct: 1    MALVPTP--VVNEG-PMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEA 57

Query: 441  SLYGAQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEEFRKYHASAIDVPGPEVERLAAM 620
            + YGAQLVVFPEAFIGGYPRGS FG++IG+RT KGKEEFRKYHASAIDVPGPEV+RLAAM
Sbjct: 58   ASYGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAM 117

Query: 621  AGKYKVYLVMGVIEREGYTLYCTILFFDPQGQYLGKHRKLMPTALERVIWGFGDGSTIPV 800
            AGKYKVYLVMGVIER+GYTLYCT+LFFD QG YLGKHRK+MPTALER+IWGFGDGSTIPV
Sbjct: 118  AGKYKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPV 177

Query: 801  FETSIGKIGALICWENKMPLLRTAMYGKGIEIYCAPTADSRELWQASMTHIALEGGCFVL 980
            ++T +GKIGA ICWEN+MPLLRTAMY KGIEIYCAPTADSR++WQASMTHIALEGGCFVL
Sbjct: 178  YDTPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVL 237

Query: 981  SANQFCRRKDYPPPPEYTFSGIDEDYSPESVVCAGGSVIISPSGTVLAGPNYEGEALISA 1160
            SANQFCRRKDYPPPPEY FSG  ED +P+S+VCAGGSVIISPSG VLAGPNYEGEALISA
Sbjct: 238  SANQFCRRKDYPPPPEYVFSG-TEDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISA 296

Query: 1161 DLDFGEIVRAKFDFDVVGHYARPDVLSLIVRDHPTTPVSFTAKTEGSQKS 1310
            DLD GEI RAKFDFDVVGHYARP+VLSLIVRDH  +PVSFT+ +  ++ S
Sbjct: 297  DLDLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESS 346


>ref|XP_002328237.1| nitrilase 1 [Populus trichocarpa] gi|222837752|gb|EEE76117.1|
            nitrilase 1 [Populus trichocarpa]
          Length = 348

 Score =  570 bits (1468), Expect = e-160
 Identities = 277/352 (78%), Positives = 305/352 (86%), Gaps = 3/352 (0%)
 Frame = +3

Query: 261  MALVPTPPSISNNGAPVIAEVDMGSDXXXXXXXXXXXXXXXIFYDTPATLDKAERLIADA 440
            MALVP PPS +    P+ AEVDM SD               +FYDTPATLDKAER +A+A
Sbjct: 1    MALVPAPPSET----PLFAEVDMCSDSSATTVRATVIQASTVFYDTPATLDKAERFLAEA 56

Query: 441  SLYGAQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEEFRKYHASAIDVPGPEVERLAAM 620
            + YG+QLVVFPEAFIGGYPRGS+FG TIGSRT KG+E+FRKYHASAIDVPGPEV+RLAAM
Sbjct: 57   AGYGSQLVVFPEAFIGGYPRGSSFGATIGSRTAKGREDFRKYHASAIDVPGPEVDRLAAM 116

Query: 621  AGKYKVYLVMGVIEREGYTLYCTILFFDPQGQYLGKHRKLMPTALERVIWGFGDGSTIPV 800
            AGKYKVYLVMGVIEREGYTLYCT+LFFD QG Y+GKHRK+MPTA+ER++WGFGDGSTIPV
Sbjct: 117  AGKYKVYLVMGVIEREGYTLYCTVLFFDSQGHYMGKHRKVMPTAVERIVWGFGDGSTIPV 176

Query: 801  FETSIGKIGALICWENKMPLLRTAMYGKGIEIYCAPTADSRELWQASMTHIALEGGCFVL 980
            F T IGKIGA ICWEN+MPLLRTAMYGKGIEIYCAPTADSR+ WQA+MTHIALEGGCFVL
Sbjct: 177  FGTPIGKIGAAICWENRMPLLRTAMYGKGIEIYCAPTADSRDTWQATMTHIALEGGCFVL 236

Query: 981  SANQFCRRKDYPPPPEYTFSGIDEDYSPESVVCAGGSVIISPSGTVLAGPNYEGEALISA 1160
            SANQFCRRKDYPPPPEY F G++ED +P+SVVCAGGSVIISP GTVLAGPNY+GEALISA
Sbjct: 237  SANQFCRRKDYPPPPEYVFLGVEEDLTPDSVVCAGGSVIISPLGTVLAGPNYDGEALISA 296

Query: 1161 DLDFGEIVRAKFDFDVVGHYARPDVLSLIVRDHPTTPVSFT---AKTEGSQK 1307
            DLD GEI RAKFDFDVVGHY+RP+VLSL VRDHPT  V FT   AKTE S+K
Sbjct: 297  DLDLGEIARAKFDFDVVGHYSRPEVLSLTVRDHPTNAVMFTSESAKTEASRK 348


>gb|AFK41272.1| unknown [Lotus japonicus]
          Length = 346

 Score =  569 bits (1466), Expect = e-159
 Identities = 273/347 (78%), Positives = 304/347 (87%)
 Frame = +3

Query: 261  MALVPTPPSISNNGAPVIAEVDMGSDXXXXXXXXXXXXXXXIFYDTPATLDKAERLIADA 440
            MALVPT     NNG P+IAEVDMGSD               IFYDTPATLDKAERL+A+A
Sbjct: 1    MALVPTTTPTINNG-PLIAEVDMGSDSNAPTVRATVVQASTIFYDTPATLDKAERLLAEA 59

Query: 441  SLYGAQLVVFPEAFIGGYPRGSNFGLTIGSRTLKGKEEFRKYHASAIDVPGPEVERLAAM 620
            + YG+QLVVFPEAFIGGYPRGSNFG+ IG RT KG+E+FRKYH+SAIDVPGPEV+RLAAM
Sbjct: 60   AGYGSQLVVFPEAFIGGYPRGSNFGIIIGIRTAKGREDFRKYHSSAIDVPGPEVDRLAAM 119

Query: 621  AGKYKVYLVMGVIEREGYTLYCTILFFDPQGQYLGKHRKLMPTALERVIWGFGDGSTIPV 800
            AGKYKV+LVMGVIER+GYTLYCT+LFFD QG YLGKHRKLMPT +ERV+WGFGDGSTIPV
Sbjct: 120  AGKYKVHLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKLMPTGMERVMWGFGDGSTIPV 179

Query: 801  FETSIGKIGALICWENKMPLLRTAMYGKGIEIYCAPTADSRELWQASMTHIALEGGCFVL 980
            FET +GKIGA+ICWEN+MPLLRTAMY KG+EIYCAPTAD+RE+WQASMTHIALEGGCFVL
Sbjct: 180  FETPLGKIGAVICWENRMPLLRTAMYAKGVEIYCAPTADAREVWQASMTHIALEGGCFVL 239

Query: 981  SANQFCRRKDYPPPPEYTFSGIDEDYSPESVVCAGGSVIISPSGTVLAGPNYEGEALISA 1160
            SANQFCRRKDYPPPPEY F+G +ED +P+SVVCAGGSVIISP G VLAGPNYEGEALISA
Sbjct: 240  SANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPLGNVLAGPNYEGEALISA 299

Query: 1161 DLDFGEIVRAKFDFDVVGHYARPDVLSLIVRDHPTTPVSFTAKTEGS 1301
            DLD GEI RAKFDFDVVGHY+RP+VLSL V+DHPT PV+F + +  S
Sbjct: 300  DLDLGEIARAKFDFDVVGHYSRPEVLSLSVKDHPTNPVTFASTSTKS 346