BLASTX nr result
ID: Cimicifuga21_contig00001112
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cimicifuga21_contig00001112
(2227 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002270550.1| PREDICTED: putative 3,4-dihydroxy-2-butanone... 861 0.0
ref|XP_002318315.1| predicted protein [Populus trichocarpa] gi|2... 858 0.0
ref|XP_002329708.1| predicted protein [Populus trichocarpa] gi|2... 846 0.0
ref|NP_001234326.1| putative 3,4-dihydroxy-2-butanone kinase [So... 844 0.0
ref|XP_003534124.1| PREDICTED: putative 3,4-dihydroxy-2-butanone... 834 0.0
>ref|XP_002270550.1| PREDICTED: putative 3,4-dihydroxy-2-butanone kinase [Vitis vinifera]
gi|297742797|emb|CBI35477.3| unnamed protein product
[Vitis vinifera]
Length = 594
Score = 861 bits (2224), Expect = 0.0
Identities = 436/594 (73%), Positives = 483/594 (81%)
Frame = +3
Query: 156 MAFQGKKLMDDPNDVVTEFIEGLVETYPGLQYLDGFPQVKVVLRADILSATYDKVVVISG 335
MAFQ KKL++DPNDVVTEFIEGLVETYPGLQYLDGFPQVKVVLRAD+ ATY+KV VISG
Sbjct: 1 MAFQAKKLINDPNDVVTEFIEGLVETYPGLQYLDGFPQVKVVLRADVSGATYNKVAVISG 60
Query: 336 GGSGHEPAHAGFVGEGMLGAAICGDVFTSPQVDSILAGIRAVTGPKGCLLIVKNYTGDRL 515
GGSGHEPAHAGFVGEGML AAICGDVF SP VDSILAGIRAVTGP G LLIVKNYTGDRL
Sbjct: 61 GGSGHEPAHAGFVGEGMLTAAICGDVFASPSVDSILAGIRAVTGPMGALLIVKNYTGDRL 120
Query: 516 NFGLAAEQAKSEGYKVETVIVGDDCALPPPRGIAGRRGLAGTXXXXXXXXXXXXXXXXXX 695
NFGLAAE+AKSEGYKVE VIVGDDCALPPPRGIAGRRGLAGT
Sbjct: 121 NFGLAAEEAKSEGYKVEIVIVGDDCALPPPRGIAGRRGLAGTILVHKVAGAAAAAGLSLA 180
Query: 696 XXXXEAKNAAEMVGTMGVALSVCTLPGQLTSDRLGPGKMELGLGIHGEPGAAVVDLLPVD 875
EA++A+EMVGTMGVAL+VCTLPGQ+TSDRLGPGKMELGLGIHGEPGAAV DL PVD
Sbjct: 181 DVAAEARHASEMVGTMGVALTVCTLPGQVTSDRLGPGKMELGLGIHGEPGAAVADLQPVD 240
Query: 876 LIVSHILQQILSKDTQYVPITRGSRVVAMINGLGATPIMELMIASGKTVPKLQVEHGLAV 1055
++VSH+L+QIL +T YVPITRG+RVV +INGLGATP MELMIA+GK VP+LQ+EHGLAV
Sbjct: 241 VVVSHVLKQILLPETNYVPITRGNRVVLLINGLGATPAMELMIAAGKAVPRLQLEHGLAV 300
Query: 1056 DRVYTGSFMTSLDMAGFSISIMKADEALLRRLDAPTTAPYWPVAVEGDRPPTKLPVPLPT 1235
DRVYTGSFMTSLDMAGFSIS+MKAD+++L+RLDA T AP+WPV V+G+RP K+PVP+P
Sbjct: 301 DRVYTGSFMTSLDMAGFSISVMKADQSILQRLDAATKAPHWPVGVDGNRPAAKIPVPMPP 360
Query: 1236 SRPRKSDEASSRPQELSVQGCVLXXXXXXXXXXXXXLKDGLNDWDSKVGDGDCGSTMFRG 1415
R KSDE+ SRPQ L+ QGC+L LKD LN+WDSK GDGDCGSTMFRG
Sbjct: 361 CRSTKSDESLSRPQLLNEQGCILEVAIEAAANSVINLKDWLNEWDSKAGDGDCGSTMFRG 420
Query: 1416 ATAILEDMRNYYPLNDAAETVNEIGSSIKRVMGGTSGIIYDIFCKAAYAKMKESNLSLVT 1595
ATAILEDM+ +YPLND AETVNEIGSSI+R MGGTSGIIYDIFCKAAYA++K + ++VT
Sbjct: 421 ATAILEDMKKFYPLNDPAETVNEIGSSIRRAMGGTSGIIYDIFCKAAYAQLKANTKTVVT 480
Query: 1596 SRQWADALEASITAISKYGGARAGYRTLLDALIPASTVLQERLAAGDEPFTAFXXXXXXX 1775
++QW DALEASI A+SKYGGA AGYRT+LDALIPA TVLQERL A D+P TAF
Sbjct: 481 AKQWKDALEASIAAVSKYGGASAGYRTMLDALIPALTVLQERLDAEDDPVTAFVLSSEAA 540
Query: 1776 XXXXXXTKHMQAQAGRSSYISAEILASVPDPGXXXXXXXXXXXXXXVKDKYQPS 1937
TKHMQAQAGRSSYIS EILASVPDPG VKDK S
Sbjct: 541 LDGAESTKHMQAQAGRSSYISGEILASVPDPGAMAAASWYRAAALAVKDKCMAS 594
>ref|XP_002318315.1| predicted protein [Populus trichocarpa] gi|222858988|gb|EEE96535.1|
predicted protein [Populus trichocarpa]
Length = 595
Score = 858 bits (2217), Expect = 0.0
Identities = 436/592 (73%), Positives = 485/592 (81%)
Frame = +3
Query: 156 MAFQGKKLMDDPNDVVTEFIEGLVETYPGLQYLDGFPQVKVVLRADILSATYDKVVVISG 335
MA QGKKL++DPNDVVTEFIEGLVETYPGLQYLDGFP+VKVVLRAD SA DKV +ISG
Sbjct: 1 MASQGKKLINDPNDVVTEFIEGLVETYPGLQYLDGFPEVKVVLRADHASAMLDKVAIISG 60
Query: 336 GGSGHEPAHAGFVGEGMLGAAICGDVFTSPQVDSILAGIRAVTGPKGCLLIVKNYTGDRL 515
GGSGHEPAHAGFVGEGML AAICG+VFTSPQVD+ILAGIRAVTGP GCLLIVKNYTGDRL
Sbjct: 61 GGSGHEPAHAGFVGEGMLSAAICGEVFTSPQVDAILAGIRAVTGPMGCLLIVKNYTGDRL 120
Query: 516 NFGLAAEQAKSEGYKVETVIVGDDCALPPPRGIAGRRGLAGTXXXXXXXXXXXXXXXXXX 695
NFGLAAEQAKSEGYKVETVIVGDDCALPPPRGIAGRRGLAGT
Sbjct: 121 NFGLAAEQAKSEGYKVETVIVGDDCALPPPRGIAGRRGLAGTILVHKVAGAAAAAGLSLD 180
Query: 696 XXXXEAKNAAEMVGTMGVALSVCTLPGQLTSDRLGPGKMELGLGIHGEPGAAVVDLLPVD 875
EAK A+EMVGTMGVALSV TLPGQ+TSDRLGPGKMELGLGIHGEPGAA+ DL PV+
Sbjct: 181 EVAAEAKRASEMVGTMGVALSVGTLPGQVTSDRLGPGKMELGLGIHGEPGAALADLQPVE 240
Query: 876 LIVSHILQQILSKDTQYVPITRGSRVVAMINGLGATPIMELMIASGKTVPKLQVEHGLAV 1055
++VSH+LQQILS +T YVPITRG+RVV ++NGLGATP MELMIA+GK VP+LQ+EHGLAV
Sbjct: 241 VVVSHVLQQILSPETNYVPITRGNRVVLLVNGLGATPGMELMIAAGKAVPQLQLEHGLAV 300
Query: 1056 DRVYTGSFMTSLDMAGFSISIMKADEALLRRLDAPTTAPYWPVAVEGDRPPTKLPVPLPT 1235
DRVYTGSFMTSLDMAGFSISIMKADEA L+RLDA T APYWPV V+G+RPP K+PVPLP
Sbjct: 301 DRVYTGSFMTSLDMAGFSISIMKADEAFLQRLDAATKAPYWPVGVDGNRPPAKIPVPLPL 360
Query: 1236 SRPRKSDEASSRPQELSVQGCVLXXXXXXXXXXXXXLKDGLNDWDSKVGDGDCGSTMFRG 1415
S KSDE+ SRPQ+LS QG +L L+D LN+WD +VGDGDCGSTM+RG
Sbjct: 361 SHSAKSDESLSRPQQLSEQGHLLEVAIEAAVNAIIDLRDNLNEWDGRVGDGDCGSTMYRG 420
Query: 1416 ATAILEDMRNYYPLNDAAETVNEIGSSIKRVMGGTSGIIYDIFCKAAYAKMKESNLSLVT 1595
ATAILEDM+ +YPLNDAAETVNEIGSSI+RVMGGTSGIIY IFCKAA ++K + S+V
Sbjct: 421 ATAILEDMKKHYPLNDAAETVNEIGSSIRRVMGGTSGIIYTIFCKAACVQLKANTQSVVA 480
Query: 1596 SRQWADALEASITAISKYGGARAGYRTLLDALIPASTVLQERLAAGDEPFTAFXXXXXXX 1775
S+QWA+ALEASI A+SKYGGA AG+RTLLDALIPAS VLQERL AGD+P TAF
Sbjct: 481 SKQWAEALEASIAAVSKYGGASAGFRTLLDALIPASAVLQERLNAGDDPCTAFLLSSEAA 540
Query: 1776 XXXXXXTKHMQAQAGRSSYISAEILASVPDPGXXXXXXXXXXXXXXVKDKYQ 1931
TKHMQAQAGRS+YIS++ILA+VPDPG +K+ YQ
Sbjct: 541 VAGAESTKHMQAQAGRSTYISSDILATVPDPGAMAAAAWYRAAAIAIKNNYQ 592
>ref|XP_002329708.1| predicted protein [Populus trichocarpa] gi|222870616|gb|EEF07747.1|
predicted protein [Populus trichocarpa]
Length = 595
Score = 846 bits (2185), Expect = 0.0
Identities = 431/592 (72%), Positives = 479/592 (80%)
Frame = +3
Query: 156 MAFQGKKLMDDPNDVVTEFIEGLVETYPGLQYLDGFPQVKVVLRADILSATYDKVVVISG 335
MA QGKKL++DPN VVTEFIEGLVETYPGLQYLDGFP+VKVVLRAD SA DKV VISG
Sbjct: 1 MASQGKKLINDPNAVVTEFIEGLVETYPGLQYLDGFPEVKVVLRADYASAMLDKVAVISG 60
Query: 336 GGSGHEPAHAGFVGEGMLGAAICGDVFTSPQVDSILAGIRAVTGPKGCLLIVKNYTGDRL 515
GGSGHEPAHAGFVGEGML AAICG+VFTSPQVD+IL+GIRAVTGP GCLLIV NYTGDRL
Sbjct: 61 GGSGHEPAHAGFVGEGMLSAAICGEVFTSPQVDAILSGIRAVTGPMGCLLIVTNYTGDRL 120
Query: 516 NFGLAAEQAKSEGYKVETVIVGDDCALPPPRGIAGRRGLAGTXXXXXXXXXXXXXXXXXX 695
NFGLAAEQAKSEGYKVETVIVGDDCALPP RGI GRRGLAGT
Sbjct: 121 NFGLAAEQAKSEGYKVETVIVGDDCALPPLRGIVGRRGLAGTILVNKIAGAAAATGLSLD 180
Query: 696 XXXXEAKNAAEMVGTMGVALSVCTLPGQLTSDRLGPGKMELGLGIHGEPGAAVVDLLPVD 875
EAK A+EMVGTMGVALSVCTLPGQ+TSDRLGPGKMELGLGIHGEPGAAV DL PV+
Sbjct: 181 EVAAEAKRASEMVGTMGVALSVCTLPGQVTSDRLGPGKMELGLGIHGEPGAAVADLQPVE 240
Query: 876 LIVSHILQQILSKDTQYVPITRGSRVVAMINGLGATPIMELMIASGKTVPKLQVEHGLAV 1055
++VSH+LQQILS D YVPIT G+RVV ++NGLGATP MELMI +GK VP+LQ+EHGLAV
Sbjct: 241 VVVSHVLQQILSPDRNYVPITPGNRVVLLVNGLGATPAMELMIVAGKAVPQLQLEHGLAV 300
Query: 1056 DRVYTGSFMTSLDMAGFSISIMKADEALLRRLDAPTTAPYWPVAVEGDRPPTKLPVPLPT 1235
DRVYTGSFMTSLDMAGFSISIMKADEA+L+RLDA T AP+WPV V+G+RPP K+PVPLP
Sbjct: 301 DRVYTGSFMTSLDMAGFSISIMKADEAILQRLDASTKAPHWPVGVDGNRPPAKIPVPLPL 360
Query: 1236 SRPRKSDEASSRPQELSVQGCVLXXXXXXXXXXXXXLKDGLNDWDSKVGDGDCGSTMFRG 1415
S KSDE+ SRPQ+LS QG +L L+D LN+WD +VGDGDCGSTM+RG
Sbjct: 361 SHSGKSDESLSRPQQLSEQGHLLEVAIEAAVNAIIDLRDNLNEWDGRVGDGDCGSTMYRG 420
Query: 1416 ATAILEDMRNYYPLNDAAETVNEIGSSIKRVMGGTSGIIYDIFCKAAYAKMKESNLSLVT 1595
ATAILEDM+ +YPLNDAAETV EIGSSI+RVMGGTSGIIY IFCKAA AK+K + S+VT
Sbjct: 421 ATAILEDMKKHYPLNDAAETVGEIGSSIRRVMGGTSGIIYTIFCKAACAKLKANTQSVVT 480
Query: 1596 SRQWADALEASITAISKYGGARAGYRTLLDALIPASTVLQERLAAGDEPFTAFXXXXXXX 1775
S+QWA+ALEASI A+SKY GA AG+RTLLDALIPAS VLQERL AGD+P AF
Sbjct: 481 SKQWAEALEASIAAVSKYSGASAGFRTLLDALIPASAVLQERLNAGDDPCIAFLLSSEAA 540
Query: 1776 XXXXXXTKHMQAQAGRSSYISAEILASVPDPGXXXXXXXXXXXXXXVKDKYQ 1931
T+HMQAQAGRS+YIS +ILA+VPDPG +K+KYQ
Sbjct: 541 VAGAESTRHMQAQAGRSAYISPDILATVPDPGAMAAASWYRAAALAIKNKYQ 592
>ref|NP_001234326.1| putative 3,4-dihydroxy-2-butanone kinase [Solanum lycopersicum]
gi|7387627|sp|O04059.1|DHBK_SOLLC RecName: Full=Putative
3,4-dihydroxy-2-butanone kinase
gi|1929056|emb|CAA72805.1| putative
3,4-dihydroxy-2-butanone kinase [Solanum lycopersicum]
Length = 594
Score = 844 bits (2180), Expect = 0.0
Identities = 426/591 (72%), Positives = 474/591 (80%)
Frame = +3
Query: 156 MAFQGKKLMDDPNDVVTEFIEGLVETYPGLQYLDGFPQVKVVLRADILSATYDKVVVISG 335
M FQ KKL++DPNDVVTEFIEGL+E YPGLQYLDGFP+VKVVLRAD+ A YDKV +ISG
Sbjct: 1 MDFQSKKLINDPNDVVTEFIEGLIENYPGLQYLDGFPEVKVVLRADVSGAKYDKVAIISG 60
Query: 336 GGSGHEPAHAGFVGEGMLGAAICGDVFTSPQVDSILAGIRAVTGPKGCLLIVKNYTGDRL 515
GGSGHEPAHAGFVGEGML AAICGDVF SP VDSILAGIRAVTGP GCLLIVKNYTGDRL
Sbjct: 61 GGSGHEPAHAGFVGEGMLTAAICGDVFASPNVDSILAGIRAVTGPMGCLLIVKNYTGDRL 120
Query: 516 NFGLAAEQAKSEGYKVETVIVGDDCALPPPRGIAGRRGLAGTXXXXXXXXXXXXXXXXXX 695
NFGLAAEQAKSEGYKVE VIVGDDCALPPPRGIAGRRGLAGT
Sbjct: 121 NFGLAAEQAKSEGYKVEMVIVGDDCALPPPRGIAGRRGLAGTLLVHKVAGAAAACGLPLA 180
Query: 696 XXXXEAKNAAEMVGTMGVALSVCTLPGQLTSDRLGPGKMELGLGIHGEPGAAVVDLLPVD 875
EAK A+EMVGTMGVALSVCT PGQ+TSDRLGPGKMELGLGIHGEPGAAV DL PVD
Sbjct: 181 DVAAEAKRASEMVGTMGVALSVCTSPGQVTSDRLGPGKMELGLGIHGEPGAAVADLQPVD 240
Query: 876 LIVSHILQQILSKDTQYVPITRGSRVVAMINGLGATPIMELMIASGKTVPKLQVEHGLAV 1055
++VSH+L++ILS +T YVPITRGSRVV +INGLGATP+MELMI +GK VP+LQ+EHGLAV
Sbjct: 241 VVVSHVLKEILSPETNYVPITRGSRVVLLINGLGATPLMELMIIAGKAVPELQLEHGLAV 300
Query: 1056 DRVYTGSFMTSLDMAGFSISIMKADEALLRRLDAPTTAPYWPVAVEGDRPPTKLPVPLPT 1235
DRVYTGSFMTSLDMAGFSIS+MKAD+A+L RLDAPT AP WPV EG+RPP K+PVPLP
Sbjct: 301 DRVYTGSFMTSLDMAGFSISVMKADQAILDRLDAPTKAPNWPVGAEGNRPPAKIPVPLPP 360
Query: 1236 SRPRKSDEASSRPQELSVQGCVLXXXXXXXXXXXXXLKDGLNDWDSKVGDGDCGSTMFRG 1415
S K ++ SRP++LS QG +L L+D LN+WD+KVGDGDCGSTMFRG
Sbjct: 361 SHSIKIEKTLSRPEKLSPQGHILETAIEAAATEVVNLRDNLNEWDNKVGDGDCGSTMFRG 420
Query: 1416 ATAILEDMRNYYPLNDAAETVNEIGSSIKRVMGGTSGIIYDIFCKAAYAKMKESNLSLVT 1595
A AILEDM+ YYPLND AETVNEIG+SI RVMGGTSGI+Y IFCKAAYAK+KE+ S+VT
Sbjct: 421 AVAILEDMKKYYPLNDPAETVNEIGASIGRVMGGTSGILYSIFCKAAYAKLKENAESVVT 480
Query: 1596 SRQWADALEASITAISKYGGARAGYRTLLDALIPASTVLQERLAAGDEPFTAFXXXXXXX 1775
+ WADALEA+I A+SKYGGA AGYRTLLDALIPA + L+ERL AGD+P AF
Sbjct: 481 AIHWADALEAAIAAVSKYGGASAGYRTLLDALIPALSALKERLNAGDDPADAFIISAEAA 540
Query: 1776 XXXXXXTKHMQAQAGRSSYISAEILASVPDPGXXXXXXXXXXXXXXVKDKY 1928
TKHMQAQAGRS+Y+ +ILASVPDPG VK+KY
Sbjct: 541 SAGAESTKHMQAQAGRSTYVPGDILASVPDPGAMAAAAWYRAAALAVKEKY 591
>ref|XP_003534124.1| PREDICTED: putative 3,4-dihydroxy-2-butanone kinase-like [Glycine
max]
Length = 592
Score = 834 bits (2154), Expect = 0.0
Identities = 423/592 (71%), Positives = 477/592 (80%)
Frame = +3
Query: 156 MAFQGKKLMDDPNDVVTEFIEGLVETYPGLQYLDGFPQVKVVLRADILSATYDKVVVISG 335
MAF KKL+DDPNDVVTEFIEGLVETYPGLQYLDGFPQVKVVLRAD+ +ATYDKV +ISG
Sbjct: 1 MAFPTKKLIDDPNDVVTEFIEGLVETYPGLQYLDGFPQVKVVLRADVSAATYDKVAIISG 60
Query: 336 GGSGHEPAHAGFVGEGMLGAAICGDVFTSPQVDSILAGIRAVTGPKGCLLIVKNYTGDRL 515
GGSGHEPAHAGFVGEGML AAICGD+F+SP VDSILAGIRAVTGPKGCLL+VKNYTGDRL
Sbjct: 61 GGSGHEPAHAGFVGEGMLTAAICGDIFSSPPVDSILAGIRAVTGPKGCLLVVKNYTGDRL 120
Query: 516 NFGLAAEQAKSEGYKVETVIVGDDCALPPPRGIAGRRGLAGTXXXXXXXXXXXXXXXXXX 695
NFGLAAE AKSEGYKVETVIVGDDCALPPPRGIAGRRGLAGT
Sbjct: 121 NFGLAAEIAKSEGYKVETVIVGDDCALPPPRGIAGRRGLAGTILVHKVAGAAAAGGLSLA 180
Query: 696 XXXXEAKNAAEMVGTMGVALSVCTLPGQLTSDRLGPGKMELGLGIHGEPGAAVVDLLPVD 875
EAK+A+E+VGTMGVAL+VCT+PGQ+ SDRLGPGKMELGLGIHGEPGAAV D+ PV+
Sbjct: 181 DVAAEAKHASEIVGTMGVALTVCTIPGQVASDRLGPGKMELGLGIHGEPGAAVADIQPVN 240
Query: 876 LIVSHILQQILSKDTQYVPITRGSRVVAMINGLGATPIMELMIASGKTVPKLQVEHGLAV 1055
++VSH+LQQILS +T YVPITRG RVV M+NGLG TP MELMI +GKTVPKLQ+EHGLAV
Sbjct: 241 VVVSHVLQQILSTETNYVPITRGERVVLMVNGLGGTPTMELMITAGKTVPKLQLEHGLAV 300
Query: 1056 DRVYTGSFMTSLDMAGFSISIMKADEALLRRLDAPTTAPYWPVAVEGDRPPTKLPVPLPT 1235
DRVYTGSFMTSLDMAGFSISIMKAD +L+RLDAPT APYWPVA +G+ PP K+PVP+P
Sbjct: 301 DRVYTGSFMTSLDMAGFSISIMKADPVILQRLDAPTKAPYWPVAADGNHPPAKIPVPIPA 360
Query: 1236 SRPRKSDEASSRPQELSVQGCVLXXXXXXXXXXXXXLKDGLNDWDSKVGDGDCGSTMFRG 1415
SR K+DE SRPQ+L+ QG +L LKD LN+WD KVGDGDCGSTM+RG
Sbjct: 361 SRTAKTDEPRSRPQQLNEQGQILEVAIEAAANAIINLKDNLNEWDGKVGDGDCGSTMYRG 420
Query: 1416 ATAILEDMRNYYPLNDAAETVNEIGSSIKRVMGGTSGIIYDIFCKAAYAKMKESNLSLVT 1595
A AILED+++ YPLNDA+ETV EIGSSI R MGGTSGIIY IF KAA++ +K S+ S VT
Sbjct: 421 AKAILEDIKD-YPLNDASETVGEIGSSIGRSMGGTSGIIYTIFFKAAHSVLKASSHSGVT 479
Query: 1596 SRQWADALEASITAISKYGGARAGYRTLLDALIPASTVLQERLAAGDEPFTAFXXXXXXX 1775
S+QWA+AL SI A+SKYGGA AGYRTLLDALIPAS+VL+E+L +GD+P TAF
Sbjct: 480 SKQWAEALATSIAAVSKYGGASAGYRTLLDALIPASSVLEEKLNSGDDPCTAFVLSSEAA 539
Query: 1776 XXXXXXTKHMQAQAGRSSYISAEILASVPDPGXXXXXXXXXXXXXXVKDKYQ 1931
T MQAQAGRS+YIS EIL+++PDPG VK KYQ
Sbjct: 540 LAGAQSTVDMQAQAGRSTYISGEILSTIPDPGAMAAATWYRAAALAVKAKYQ 591