BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cimicifuga21_contig00000736
         (3262 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265386.1| PREDICTED: DNA topoisomerase 1-like [Vitis v...  1021   0.0  
dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]              1019   0.0  
dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]               1010   0.0  
dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]      1003   0.0  
dbj|BAK32958.1| topoisomerase I [Ophiorrhiza pedunculata]             987   0.0  

>ref|XP_002265386.1| PREDICTED: DNA topoisomerase 1-like [Vitis vinifera]
          Length = 883

 Score = 1021 bits (2641), Expect = 0.0
 Identities = 554/927 (59%), Positives = 639/927 (68%), Gaps = 22/927 (2%)
 Frame = -2

Query: 3003 DSSDDDGPVTFKRSKTTSKQS----------LLNAEVKKLPPQKTDGPSQKQASDMCTPN 2854
            + S+DD P++ K S    K +          +L ++  K+  +K+DG S+ +        
Sbjct: 17   EDSEDDKPLSAKLSARLPKGNSHVLKGLSSPVLQSQKTKVDVKKSDGDSEDEVP------ 70

Query: 2853 GQKSTAQKGKVVSSLKTSASKAIGTNSKESTSLADXXXXXXXXXXXSYDMEKKRSDKPP- 2677
                 + K  + + +++S+ K++G+    S SL                     S  PP 
Sbjct: 71   ----LSSKFPLKTYVRSSSGKSLGSKGLNSPSL---------------------SSHPPE 105

Query: 2676 --VVPNSDSEDSDDDKPXXXXXXXXXXXSPKSNSTNISKGLTAXXXXXXXXXXXXXXXKG 2503
              V     S+DS+D+ P              S+    SKGL++               K 
Sbjct: 106  TKVGVKESSDDSEDEIPLLLKLQLKSRKGASSSKLLDSKGLSSPASSRPQSQKAKVNNKN 165

Query: 2502 SYEDSEDGIPLSSKFHMKPGGSASIKKSYDSDEEKPLXXXXXXXXXXXXXXXXXXDEEKP 2323
              +DSED IPLSSKF +K G  AS+ K                            DE+KP
Sbjct: 166  LSDDSEDEIPLSSKFRLKTGMGASLDK------------------------LDNFDEKKP 201

Query: 2322 LATKFMQNGSVRRDNELLKSLKISNKRPL--SDTNNQSSVKKPKPSDASISNKVEHVSVK 2149
            L +    NG  ++D+E +KS K  NKRPL  ++T++ SS KKPK  D S+  KV+ VSVK
Sbjct: 202  LTSTLKPNGISKKDDERVKSPKGQNKRPLGQANTSHHSSSKKPKLLDGSVKGKVKQVSVK 261

Query: 2148 AETKTEXXXXXXD----VPIAQRMKKSTASDSKSQPMKKAPTKVVXXXXXXXXXXXXXXX 1981
            AE K +      D     P  +R+KKS +SD+K    K + TKV+               
Sbjct: 262  AEQKDDDDDDNDDDDEYTPTVKRLKKSVSSDNK----KASKTKVIPSSRKKATKKAKKSK 317

Query: 1980 XXXXXXXXXK---LPPGSGEGQKWTTLVHSGVIFPPPYKPHGVKMLYNGQPIDLTPEQEE 1810
                     K   +PP SGEGQKWTTL H+GVIFPPPYKPHGVKMLYNG+P+DLTPEQEE
Sbjct: 318  KLAKSSKYSKSTKVPPNSGEGQKWTTLEHNGVIFPPPYKPHGVKMLYNGRPVDLTPEQEE 377

Query: 1809 VATMFAVMKDTDYATKPKFLENFMNDWRVILGKNHVIKKFELCDFTPIYEWHXXXXXXXX 1630
            VATMFAVMKDTDYATKP+F+ENFMNDWRV LGKNHVIKKFELCDFTPIYEWH        
Sbjct: 378  VATMFAVMKDTDYATKPRFIENFMNDWRVRLGKNHVIKKFELCDFTPIYEWHEKEKEKKK 437

Query: 1629 XXXXXXXXXXXXXXXXXXXKYTWAFVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI 1450
                               KY WA VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI
Sbjct: 438  QMTTEEKKALKEEKLKQEDKYMWAVVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI 497

Query: 1449 RPSDITINIGKGAPVPECPIPGERWKEVKHDNTVTWLAFWIDPINPKEFKYVFLAASSSL 1270
             PSDITINIGK APVPECPIPGERWKEV+HDNTVTWLAFW DPINPKEFKYVFLAASSSL
Sbjct: 498  CPSDITINIGKEAPVPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSL 557

Query: 1269 KGQSDKEKYEKARLLKNYIHNIRENYTRDFVNKDVTKRQIAVATYLIDKLALRAGNEKDD 1090
            KGQSDKEKYEKARLLK+YI NIR  YT+DF NKD  KRQIAVATYLIDKLALRAGNEKDD
Sbjct: 558  KGQSDKEKYEKARLLKDYIQNIRATYTKDFTNKDTMKRQIAVATYLIDKLALRAGNEKDD 617

Query: 1089 DEAETVGCCTLKVDNVALIPPDKLEFDFLGKDSVRYFNTVKVELPVYKAVGQFKSGKKEG 910
            DEA+TVGCCTLKV+NVAL+PP+KLEFDFLGKDS+RYFNTV+VELPVYKA+GQFK+GK  G
Sbjct: 618  DEADTVGCCTLKVENVALVPPNKLEFDFLGKDSIRYFNTVEVELPVYKAIGQFKTGKSGG 677

Query: 909  EDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKETKGGGHVTEKIAVYQH 730
            +DLFDKLDTSKLNAHLKELMPGLTAKVFRTYNASITLD MLN+E + GG   EKI +YQH
Sbjct: 678  DDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNASITLDEMLNREAR-GGDAMEKIVIYQH 736

Query: 729  ANKEVAIICNHQRAVGKGHDSQMSKLNEKLDELKTALDGLNEDLMRAKKGKPPLKDADGK 550
            ANKEVAIICNHQR+V K HD QMS+L+EK+ EL+  LD L  DL RAKKGKPPLKD+DGK
Sbjct: 737  ANKEVAIICNHQRSVSKSHDVQMSRLSEKMRELQGVLDELKTDLARAKKGKPPLKDSDGK 796

Query: 549  QKKNLTPEALEKKIAQINVKMDKIEFDMKSKEDLKTVALGTSKINYLDPRISVAWCKREE 370
             KKN+TPE LE+KIAQ N K++K+E DMK+KEDLKTVALGTSKINYLDPRISVAWCKR+E
Sbjct: 797  PKKNMTPEVLERKIAQTNTKIEKMERDMKTKEDLKTVALGTSKINYLDPRISVAWCKRQE 856

Query: 369  VPIEKIFNKSLLAKFAWAMDVDPDFRF 289
            VPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 857  VPIEKIFNKSLLAKFAWAMDVDPSFRF 883


>dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]
          Length = 894

 Score = 1019 bits (2635), Expect = 0.0
 Identities = 552/932 (59%), Positives = 638/932 (68%), Gaps = 14/932 (1%)
 Frame = -2

Query: 3042 MAVDACVKQSFVDDSSDDDGPVTFKRSKTTSKQSLLNAEVKKLPPQKTDGPSQKQASDMC 2863
            MAV ACVK+  +D+  +DD P+ FKRS  TSK + LN++ KK   Q+ DG S +Q SD+ 
Sbjct: 1    MAVQACVKEKMMDEIDEDDEPLVFKRSNATSKHNQLNSDTKK-SSQRYDGQSGRQVSDVP 59

Query: 2862 TPNGQKSTAQKGKVVSSLKTSASKAIGTNSKESTSLADXXXXXXXXXXXSYDMEKKRSDK 2683
            + NGQ S+A KGK     K S+ K+   + K STS A            S D   K S K
Sbjct: 60   STNGQSSSASKGKTT---KLSSMKSPIASPKASTSFAKASPVANPKVSSSSDDRSKHSSK 116

Query: 2682 PPVVPNSDSE------------DSDDDKPXXXXXXXXXXXSPKSNSTNISKGLTAXXXXX 2539
               +     E            DS+D+KP             K +S N +KGL       
Sbjct: 117  QNTINVVKEEKELVNPAAEPYGDSEDEKPLSARLFTGLT---KGSSNNANKGLINSSPAS 173

Query: 2538 XXXXXXXXXXKGSYEDSEDGIPLSSKFHMKPGGSASIKKSYDSDEEKPLXXXXXXXXXXX 2359
                          +DS+D IPLSSKF +K     S  KSY+SD+ K L           
Sbjct: 174  LPVPKPEINRYS--DDSDDEIPLSSKFRLKANAGTSTVKSYESDDNKTL----------- 220

Query: 2358 XXXXXXXDEEKPLATKFMQNGSVRRDNELLKSLKISNKRPLSDTNN--QSSVKKPKPSDA 2185
                          + F QNGS+ R ++   S+K+ NKRPL +  +  QSSVKKPK SDA
Sbjct: 221  -------------VSNFQQNGSINRGSK--SSIKV-NKRPLGEVKSSVQSSVKKPKLSDA 264

Query: 2184 SISNKVEHVSVKAETKTEXXXXXXDVPIAQRMKKSTASDSKSQPMKKAPTKVVXXXXXXX 2005
            S     +  S KAE K +       VPI+QRMKKS  S +KS   K+  TKVV       
Sbjct: 265  STPVNNKQASKKAEPKADDSDDDH-VPISQRMKKSATSGNKSSTAKQKVTKVVSSSMKKA 323

Query: 2004 XXXXXXXXXXXXXXXXXKLPPGSGEGQKWTTLVHSGVIFPPPYKPHGVKMLYNGQPIDLT 1825
                             K+PP SGEG+KW TLVH+GVIFPPPYKPHGVKMLY G+P+DLT
Sbjct: 324  SKKFKKVVKKSKYSKSSKVPPSSGEGKKWNTLVHNGVIFPPPYKPHGVKMLYKGKPVDLT 383

Query: 1824 PEQEEVATMFAVMKDTDYATKPKFLENFMNDWRVILGKNHVIKKFELCDFTPIYEWHXXX 1645
            PEQEEVATMFAVM DTDY TK KF ENFM+DWR ILGKNHVI+  E CDFTPIYEWH   
Sbjct: 384  PEQEEVATMFAVMLDTDYMTKSKFKENFMDDWRKILGKNHVIQNLEDCDFTPIYEWHQRE 443

Query: 1644 XXXXXXXXXXXXXXXXXXXXXXXXKYTWAFVDGVKEKVGNFRVEPPGLFRGRGEHPKMGK 1465
                                    KY WA V+GVKEKVGNFRVEPP LFRGRGEHPKMGK
Sbjct: 444  KEKKKKMTAEEKKVLKEERLKQEEKYMWAIVNGVKEKVGNFRVEPPSLFRGRGEHPKMGK 503

Query: 1464 LKKRIRPSDITINIGKGAPVPECPIPGERWKEVKHDNTVTWLAFWIDPINPKEFKYVFLA 1285
            LKK IRPSDITINIGK AP+PECPIPGE WKE++HDNTVTWLAFW DPI P+EFKYVFLA
Sbjct: 504  LKKCIRPSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAFWNDPIKPREFKYVFLA 563

Query: 1284 ASSSLKGQSDKEKYEKARLLKNYIHNIRENYTRDFVNKDVTKRQIAVATYLIDKLALRAG 1105
            ASSSLKGQSDKEKYEKARLLK++I  IR  YT+DF +KD+TKRQIAVATYLIDKLALRAG
Sbjct: 564  ASSSLKGQSDKEKYEKARLLKDFIQGIRAAYTKDFASKDITKRQIAVATYLIDKLALRAG 623

Query: 1104 NEKDDDEAETVGCCTLKVDNVALIPPDKLEFDFLGKDSVRYFNTVKVELPVYKAVGQFKS 925
            NEKDDDEA+TVGCCTLKV+NV   PP  L+FDFLGKDS+RY N V+VELPVYKA+ QF++
Sbjct: 624  NEKDDDEADTVGCCTLKVENVEPKPPSILKFDFLGKDSIRYQNEVEVELPVYKAIQQFRT 683

Query: 924  GKKEGEDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKETKGGGHVTEKI 745
            GK+ G+DLFDKLDTSKLNAHLK LMPGLTAKVFRTYNASITLD ML++ETK GG+V EKI
Sbjct: 684  GKRGGDDLFDKLDTSKLNAHLKGLMPGLTAKVFRTYNASITLDEMLSRETK-GGNVAEKI 742

Query: 744  AVYQHANKEVAIICNHQRAVGKGHDSQMSKLNEKLDELKTALDGLNEDLMRAKKGKPPLK 565
             VYQHANKEVAIICNHQR V K H +QM++LN K+DELK  LDGL  DL RAKKGKPPL+
Sbjct: 743  VVYQHANKEVAIICNHQRTVSKSHPAQMTRLNGKIDELKGILDGLQTDLARAKKGKPPLE 802

Query: 564  DADGKQKKNLTPEALEKKIAQINVKMDKIEFDMKSKEDLKTVALGTSKINYLDPRISVAW 385
            DADGK K+NLTPEALE+KI Q N K++K+E D ++KE LKT+ALGTSKI+YLDPRI+VAW
Sbjct: 803  DADGKPKRNLTPEALERKIGQTNAKIEKMERDKETKEGLKTIALGTSKISYLDPRITVAW 862

Query: 384  CKREEVPIEKIFNKSLLAKFAWAMDVDPDFRF 289
            CKR EVPIEK+FNKSLLAKFAW+MDVDP FRF
Sbjct: 863  CKRHEVPIEKVFNKSLLAKFAWSMDVDPSFRF 894


>dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]
          Length = 927

 Score = 1010 bits (2612), Expect = 0.0
 Identities = 569/965 (58%), Positives = 639/965 (66%), Gaps = 47/965 (4%)
 Frame = -2

Query: 3042 MAVDACVKQSFVDDSSDDDGPVTFKRSKTTSKQSLLNAEVKKLPPQKTDGPSQKQASDMC 2863
            MAV+AC   + ++D  DD+GPV FKRS   SKQ+  N+E KKL  QK  G S    SD+ 
Sbjct: 1    MAVEACTP-NLMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVR 59

Query: 2862 TPNGQKSTAQKGKVVSSLKT--------------SASKAI-----GTNSKESTSLADXXX 2740
              NG+ S+AQKG++  S KT              S++KA      G NSK S S      
Sbjct: 60   PANGESSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 119

Query: 2739 XXXXXXXXSYDMEKKRSDKPPVVPNSDSEDSDDDKPXXXXXXXXXXXSPKSNSTNISKGL 2560
                    +  +E K S  P    NS+SE SDD+KP             KSN  N   G 
Sbjct: 120  QQNHHKSSAAFIEPKPS-VPAAKANSNSESSDDEKPLSARTFGCSSKG-KSNHANKEGGD 177

Query: 2559 TAXXXXXXXXXXXXXXXKGSYEDSEDGIPLSSKFHMKPGGSASIKKSYDSDEEKPLXXXX 2380
            +                    EDSED  PLS +F  K     S  KSYDSDE+KPL    
Sbjct: 178  SCSIRRRVIEES---------EDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPL---- 224

Query: 2379 XXXXXXXXXXXXXXDEEKPLATKFMQNGSVRRDNELLKSLKISNKRPLSDTN--NQSSVK 2206
                                A K  QNGSV RD  + KS  +SNKRP  +    NQSSVK
Sbjct: 225  --------------------AAKVQQNGSVSRDGPMNKSASLSNKRPPGEVKSLNQSSVK 264

Query: 2205 KPKPSD--ASISNKVEHVSVKAETKTEXXXXXXD----------------------VPIA 2098
            KPK S    SISNK   V  + E K E                             VPIA
Sbjct: 265  KPKLSSPITSISNKQAAVKPEPEPKAEDDDDNIPLSQRGKKSQAATSKPKDEDDDNVPIA 324

Query: 2097 QRMKKSTASDSKSQ-PMKKAPTKVVXXXXXXXXXXXXXXXXXXXXXXXXKLPPGSGEGQK 1921
            QR+KKS AS SKS  P+KKA T VV                        K+PPGSGEGQK
Sbjct: 325  QRIKKSPASVSKSSAPVKKAST-VVSPSLKKVNKNSKKVIKKTAYTKSSKVPPGSGEGQK 383

Query: 1920 WTTLVHSGVIFPPPYKPHGVKMLYNGQPIDLTPEQEEVATMFAVMKDTDYATKPKFLENF 1741
            WTTLVH+GVIFPPPY PHGVKMLY  QPI LTPEQEEVATMFAVM DTDY  KP+F ENF
Sbjct: 384  WTTLVHNGVIFPPPYNPHGVKMLYKRQPITLTPEQEEVATMFAVMLDTDYMNKPRFKENF 443

Query: 1740 MNDWRVILGKNHVIKKFELCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYTW 1561
             +DW+ +LGKNH I+  E CDF PIYEWH                           KY W
Sbjct: 444  FSDWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKKKQMTTEEKKALKEDKLQQEEKYMW 503

Query: 1560 AFVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKGAPVPECPIPGE 1381
            A VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRP DITINIGK AP+PECPIPGE
Sbjct: 504  AIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKDAPIPECPIPGE 563

Query: 1380 RWKEVKHDNTVTWLAFWIDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKNYIHNIR 1201
            RWKEV++DNTVTWLAFWIDPIN KEFKYVFLAASS+LKGQSDKEKYEKARLLK+YIH IR
Sbjct: 564  RWKEVRNDNTVTWLAFWIDPINQKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIHGIR 623

Query: 1200 ENYTRDFVN-KDVTKRQIAVATYLIDKLALRAGNEKDDDEAETVGCCTLKVDNVALIPPD 1024
              YT+DF N KD  K+QIAVATYLIDKLALRAGNEKDDDEA+TVGCCTLKV+NV  +PP+
Sbjct: 624  AAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN 683

Query: 1023 KLEFDFLGKDSVRYFNTVKVELPVYKAVGQFKSGKKEGEDLFDKLDTSKLNAHLKELMPG 844
             L+FDFLGKDS+RY N V+VE  V+KA+ QF+SGK+  EDLFD+LDTSKLNAHLKELMPG
Sbjct: 684  ILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGSEDLFDRLDTSKLNAHLKELMPG 743

Query: 843  LTAKVFRTYNASITLDGMLNKETKGGGHVTEKIAVYQHANKEVAIICNHQRAVGKGHDSQ 664
            LTAKVFRTYNASITLD ML+KETKGG  V EK+ VYQHANKEVAIICNHQR V K H +Q
Sbjct: 744  LTAKVFRTYNASITLDDMLSKETKGG-EVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQ 802

Query: 663  MSKLNEKLDELKTALDGLNEDLMRAKKGKPPLKDADGKQKKNLTPEALEKKIAQINVKMD 484
            MS+LNEK+DELKTA++ L  DL R KKGKPP K ADGK K+NL PEALE+K+AQIN K++
Sbjct: 803  MSRLNEKIDELKTAMEELKTDLSRVKKGKPPSKGADGKPKRNLNPEALERKMAQINAKIE 862

Query: 483  KIEFDMKSKEDLKTVALGTSKINYLDPRISVAWCKREEVPIEKIFNKSLLAKFAWAMDVD 304
            K+E D ++KEDLKTVALGTSKINYLDPRI+VAWCKR EVPIEKIFNKSLLAKFAW+MDVD
Sbjct: 863  KMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWSMDVD 922

Query: 303  PDFRF 289
            P FRF
Sbjct: 923  PSFRF 927


>dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]
          Length = 925

 Score = 1003 bits (2594), Expect = 0.0
 Identities = 568/965 (58%), Positives = 636/965 (65%), Gaps = 47/965 (4%)
 Frame = -2

Query: 3042 MAVDACVKQSFVDDSSDDDGPVTFKRSKTTSKQSLLNAEVKKLPPQKTDGPSQKQASDMC 2863
            MAV+AC   + ++D  DD GPV FKRSK   KQ+  N+E KKL  QK DG S    SD+ 
Sbjct: 1    MAVEACTT-NLMEDMEDDGGPVIFKRSKPAPKQNQANSEKKKLSLQKQDGQSAVHTSDVR 59

Query: 2862 TPNGQKSTAQKGKVVSSLKT--------------SASKAI-----GTNSKESTSLADXXX 2740
              NG+ S+AQKG++  S KT              S++KA      G NSK S S      
Sbjct: 60   PANGESSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 119

Query: 2739 XXXXXXXXSYDMEKKRSDKPPVVPNSDSEDSDDDKPXXXXXXXXXXXSPKSNSTNISKGL 2560
                    +  +E K S  P    NS+SE SDD+KP             KSN  N   G 
Sbjct: 120  QQNHHKSSAAFIEPKPS-VPAAKANSNSESSDDEKPLSARTFGCSSKG-KSNHANKEGGD 177

Query: 2559 TAXXXXXXXXXXXXXXXKGSYEDSEDGIPLSSKFHMKPGGSASIKKSYDSDEEKPLXXXX 2380
            +                    EDSED  PLS +F  K     S  KSYDSDE+KPL    
Sbjct: 178  SCSIRRPVIEES---------EDSEDEKPLSCRFTSKSSLGESTSKSYDSDEKKPL---- 224

Query: 2379 XXXXXXXXXXXXXXDEEKPLATKFMQNGSVRRDNELLKSLKISNKRPLSDTN--NQSSVK 2206
                                A K  QNGS  RD  + KS  +SNKRP  +    NQSSVK
Sbjct: 225  --------------------AAKVQQNGSASRDGPMNKSASLSNKRPPGEAKSLNQSSVK 264

Query: 2205 KPKPSD--ASISNKVEHVSVKAETKTEXXXXXXD----------------------VPIA 2098
            KPK S    SISNK    +VK E K E                             VPI+
Sbjct: 265  KPKLSSPITSISNK--QAAVKPEPKAEDDDDNIPLSQRGKKSQSATSKPKDDDDDNVPIS 322

Query: 2097 QRMKKSTASDSKSQ-PMKKAPTKVVXXXXXXXXXXXXXXXXXXXXXXXXKLPPGSGEGQK 1921
            QR+KKS AS SKS  P+KKA T VV                        K+PPGSGEGQK
Sbjct: 323  QRIKKSPASVSKSSVPVKKAST-VVSSSLKKVNKNSKKVIKKTAYTKSSKVPPGSGEGQK 381

Query: 1920 WTTLVHSGVIFPPPYKPHGVKMLYNGQPIDLTPEQEEVATMFAVMKDTDYATKPKFLENF 1741
            WTTLVH+GVIFPPPYKPHGVKMLY  QPI LTPEQEEVATMFAVM DTDY  KP F ENF
Sbjct: 382  WTTLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEVATMFAVMLDTDYMNKPCFKENF 441

Query: 1740 MNDWRVILGKNHVIKKFELCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYTW 1561
             +DW+ ILGKNH+I+  E CDF PIYEWH                           KY W
Sbjct: 442  FSDWKKILGKNHMIQNLEDCDFGPIYEWHQQEKEKKKQMTTEEKKALKEEKLQQEEKYMW 501

Query: 1560 AFVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKGAPVPECPIPGE 1381
            A VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRP DITINIGK AP+PECPIPGE
Sbjct: 502  AIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKDAPIPECPIPGE 561

Query: 1380 RWKEVKHDNTVTWLAFWIDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKNYIHNIR 1201
            RWKEV++ NTVTWLA+W DPIN KEFKYVFLAASS+LKGQSDKEKYEKARLLK+YIH IR
Sbjct: 562  RWKEVRNGNTVTWLAYWNDPINQKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIHGIR 621

Query: 1200 ENYTRDFVN-KDVTKRQIAVATYLIDKLALRAGNEKDDDEAETVGCCTLKVDNVALIPPD 1024
              YT+DF N KD  K+QIAVATYLIDKLALRAGNEKDDDEA+TVGCCTLKV+NV  +PP+
Sbjct: 622  AAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN 681

Query: 1023 KLEFDFLGKDSVRYFNTVKVELPVYKAVGQFKSGKKEGEDLFDKLDTSKLNAHLKELMPG 844
             L+FDFLGKDS+RY N V+VE  V+KA+ QF+SGK+  EDLFD+LDTSKLNAHLKELMPG
Sbjct: 682  ILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGSEDLFDRLDTSKLNAHLKELMPG 741

Query: 843  LTAKVFRTYNASITLDGMLNKETKGGGHVTEKIAVYQHANKEVAIICNHQRAVGKGHDSQ 664
            LTAKVFRTYNASITLD ML+KETKGG  V E + VYQHANKEVAIICNHQR V K H +Q
Sbjct: 742  LTAKVFRTYNASITLDDMLSKETKGG-EVAENVVVYQHANKEVAIICNHQRTVSKSHSAQ 800

Query: 663  MSKLNEKLDELKTALDGLNEDLMRAKKGKPPLKDADGKQKKNLTPEALEKKIAQINVKMD 484
            MS+LNEK+DELKTAL+ L  DL RAKKGKPP K ADGK K+NL PEALE+KI Q N K++
Sbjct: 801  MSRLNEKIDELKTALEELKTDLSRAKKGKPPTKGADGKAKRNLNPEALERKITQTNAKIE 860

Query: 483  KIEFDMKSKEDLKTVALGTSKINYLDPRISVAWCKREEVPIEKIFNKSLLAKFAWAMDVD 304
            K+E D ++KEDLKTVALGTSKINYLDPRI+VAWCKR EVPIEKIFNKSLLAKFAW+MDVD
Sbjct: 861  KMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWSMDVD 920

Query: 303  PDFRF 289
            P FRF
Sbjct: 921  PSFRF 925


>dbj|BAK32958.1| topoisomerase I [Ophiorrhiza pedunculata]
          Length = 920

 Score =  987 bits (2551), Expect = 0.0
 Identities = 558/958 (58%), Positives = 631/958 (65%), Gaps = 47/958 (4%)
 Frame = -2

Query: 3042 MAVDACVKQSFVDDSSDDDGPVTFKRSKTTSKQSLLNAEVKKLPPQKTDGPSQKQASDMC 2863
            MAV+AC   + ++D  DD+GPV FKRS   SKQ+  N+E KKL  QK  G S    SD+ 
Sbjct: 1    MAVEACTTTNLMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVR 60

Query: 2862 TPNGQKSTAQKGKVVSSLKT--------------SASKAI-----GTNSKESTSLADXXX 2740
              NG+ S+AQKG++  S KT              S++KA      G NSK S S      
Sbjct: 61   PANGENSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 120

Query: 2739 XXXXXXXXSYDMEKKRSDKPPVVPNSDSEDSDDDKPXXXXXXXXXXXSPKSNSTNISKGL 2560
                    +  +E K S  P    NS+SE SDD+KP             KSN  N   G 
Sbjct: 121  QQNHHKSSAAFIEPKPS-VPAAKANSNSESSDDEKPLSARTFGCSSKG-KSNHANKEGGD 178

Query: 2559 TAXXXXXXXXXXXXXXXKGSYEDSEDGIPLSSKFHMKPGGSASIKKSYDSDEEKPLXXXX 2380
            +                    EDSED  PLS +F  K     S  KSYDSDE+KPL    
Sbjct: 179  SCSIRRPVIEES---------EDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPL---- 225

Query: 2379 XXXXXXXXXXXXXXDEEKPLATKFMQNGSVRRDNELLKSLKISNKRPLSDTN--NQSSVK 2206
                                A K   NGS  RD+ + KS  +SNKRP  +    NQSS+K
Sbjct: 226  --------------------AAKVQPNGSASRDSPMNKSASLSNKRPPGEVKSLNQSSLK 265

Query: 2205 KPKPSD--ASISNKVEHVSVKAETKTEXXXXXXD----------------------VPIA 2098
            KPK S    SISNK    +VK E K E                             VPI+
Sbjct: 266  KPKLSSPITSISNK--QAAVKPEPKAEDDDDNIPLSQRGKKSQTATSKPKDEDDDNVPIS 323

Query: 2097 QRMKKSTASDSKSQ-PMKKAPTKVVXXXXXXXXXXXXXXXXXXXXXXXXKLPPGSGEGQK 1921
            QR+KKS AS SKS  P+KKA T VV                        K+PPGSGEGQK
Sbjct: 324  QRIKKSPASVSKSSAPVKKAST-VVSPSLKKVNKKSKKVIKKTAYTKSSKVPPGSGEGQK 382

Query: 1920 WTTLVHSGVIFPPPYKPHGVKMLYNGQPIDLTPEQEEVATMFAVMKDTDYATKPKFLENF 1741
            WTTLVH+GVIFPPPYKPHGVKMLY  QPI LTPEQEEVATMFAVM DTDY  KP+F ENF
Sbjct: 383  WTTLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEVATMFAVMLDTDYMNKPRFKENF 442

Query: 1740 MNDWRVILGKNHVIKKFELCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYTW 1561
             +DW+ +LGKNH I+  E CDF PIYEWH                           KY W
Sbjct: 443  FSDWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKKKQMTTEEKKALKEEKLQQEEKYMW 502

Query: 1560 AFVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKGAPVPECPIPGE 1381
            A VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRP DITINIGK AP+PECPIPGE
Sbjct: 503  AIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKDAPIPECPIPGE 562

Query: 1380 RWKEVKHDNTVTWLAFWIDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKNYIHNIR 1201
            RWKEV++DNTVTWLA+W DPIN KEFKYVFLAASS+LKGQSDKEKYEKARLLK+YIH IR
Sbjct: 563  RWKEVRNDNTVTWLAYWNDPINQKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIHGIR 622

Query: 1200 ENYTRDFV-NKDVTKRQIAVATYLIDKLALRAGNEKDDDEAETVGCCTLKVDNVALIPPD 1024
              YT+DF  NKD  K+QIAVATYLIDKLALRAGNEKDDDEA+TVGCCTLKV+NV  +PP+
Sbjct: 623  AAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN 682

Query: 1023 KLEFDFLGKDSVRYFNTVKVELPVYKAVGQFKSGKKEGEDLFDKLDTSKLNAHLKELMPG 844
             L+FDFLGKDS+RY N V+VE  V+KA+ QF+SGK+  EDLFD+LDTSKLNAHLKELMPG
Sbjct: 683  ILKFDFLGKDSIRYQNEVQVEPAVFKAIHQFRSGKEGSEDLFDRLDTSKLNAHLKELMPG 742

Query: 843  LTAKVFRTYNASITLDGMLNKETKGGGHVTEKIAVYQHANKEVAIICNHQRAVGKGHDSQ 664
            LTAKVFRTYNASITLD ML+K+T  GG V EK+ VYQHANKEVAIICNHQR V K H +Q
Sbjct: 743  LTAKVFRTYNASITLDDMLSKKT-NGGEVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQ 801

Query: 663  MSKLNEKLDELKTALDGLNEDLMRAKKGKPPLKDADGKQKKNLTPEALEKKIAQINVKMD 484
            MS+LNEK+DELKTAL+ L  DL RAKKGKP  K ADGK K+NL PEALE+KIAQ N K++
Sbjct: 802  MSRLNEKIDELKTALEELKTDLSRAKKGKPLSKGADGKPKRNLNPEALERKIAQTNAKIE 861

Query: 483  KIEFDMKSKEDLKTVALGTSKINYLDPRISVAWCKREEVPIEKIFNKSLLAKFAWAMD 310
            K+E D ++KEDLKTVALGTSKINYLDPRI+VAWCKR EVPIEKIFNKSLLAKFAWAM+
Sbjct: 862  KMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMN 919