BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00046509
         (558 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_023753869.1| two-pore potassium channel 1 [Lactuca sativa...   152   1e-41
ref|XP_021978220.1| two-pore potassium channel 1-like [Helianthu...   151   3e-41
ref|XP_021978053.1| two-pore potassium channel 1-like [Helianthu...   150   1e-40
gb|KVH99181.1| EF-Hand 1, calcium-binding site-containing protei...   147   2e-37
ref|XP_023740032.1| two-pore potassium channel 1-like [Lactuca s...   134   1e-34
ref|XP_023740005.1| two-pore potassium channel 1-like [Lactuca s...   127   6e-32
gb|KVI02335.1| EF-Hand 1, calcium-binding site-containing protei...   122   9e-30
ref|XP_022001523.1| two-pore potassium channel 1-like [Helianthu...   119   8e-29
gb|PLY93033.1| hypothetical protein LSAT_5X1461 [Lactuca sativa]      116   3e-28
gb|OTG19180.1| putative two pore domain potassium channel [Helia...   115   1e-27
gb|PNT58681.1| hypothetical protein POPTR_001G366800v3 [Populus ...   114   6e-27
ref|XP_006370002.1| pulvinus outward-rectifying channel for pota...   114   6e-27
ref|XP_023524757.1| two-pore potassium channel 1-like [Cucurbita...   113   1e-26
ref|XP_022939985.1| two-pore potassium channel 1-like [Cucurbita...   113   1e-26
gb|KZM95607.1| hypothetical protein DCAR_018849 [Daucus carota s...   112   2e-26
ref|XP_024199683.1| two-pore potassium channel 1-like [Rosa chin...   112   2e-26
ref|XP_017249114.1| PREDICTED: two-pore potassium channel 1-like...   112   2e-26
ref|XP_008462994.1| PREDICTED: two-pore potassium channel 1 isof...   112   3e-26
ref|XP_011038782.1| PREDICTED: two-pore potassium channel 1 [Pop...   112   4e-26
ref|XP_022981526.1| two-pore potassium channel 1-like [Cucurbita...   112   4e-26

>ref|XP_023753869.1| two-pore potassium channel 1 [Lactuca sativa]
 ref|XP_023753871.1| two-pore potassium channel 1 [Lactuca sativa]
          Length = 355

 Score =  152 bits (385), Expect = 1e-41
 Identities = 86/178 (48%), Positives = 109/178 (61%), Gaps = 1/178 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           L QT+   +  K++E N  RNKC                 LV VEDLDFIHA YC++ATI
Sbjct: 159 LNQTLGTAETLKQMETNKVRNKCIILVALLVVLMAAGTTVLVAVEDLDFIHAFYCVVATI 218

Query: 376 IGVGS-DRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXX 200
             +G  D+CFSTKGGRVFALFW+LSGT+YLG+LL+ F  L TQ R+R   K A+K K   
Sbjct: 219 TSLGYIDKCFSTKGGRVFALFWILSGTIYLGQLLFTFAMLHTQRRQRSLVKWALKRK-TT 277

Query: 199 XXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                      DGV+ A+E+I++ L E GKISQEDMAPI+++F+ LD D    L   D
Sbjct: 278 PADLEAADLDDDGVVVAAEFILYKLKEMGKISQEDMAPIMEEFERLDFDKTGTLTASD 335


>ref|XP_021978220.1| two-pore potassium channel 1-like [Helianthus annuus]
 gb|OTG19371.1| putative two pore domain potassium channel, EF-hand domain pair
           [Helianthus annuus]
          Length = 365

 Score =  151 bits (382), Expect = 3e-41
 Identities = 83/178 (46%), Positives = 108/178 (60%), Gaps = 1/178 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           ++QTV   +  KR+E N  RNKC                 LV VEDLDF+HA YC++ TI
Sbjct: 169 MHQTVGTAETLKRMESNRVRNKCILLVVLLVVLMGAGTAVLVAVEDLDFVHAFYCVVGTI 228

Query: 376 IGVGS-DRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXX 200
             +G  D+CFSTKGGRVFALFW+LSGT+YLG+LL+ F  L TQ R++   K A+K K   
Sbjct: 229 TSLGYIDKCFSTKGGRVFALFWILSGTIYLGQLLFTFAVLHTQRRQKSLVKWALKRK-TT 287

Query: 199 XXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                      DGV+ A+E+I++ L E GKISQEDM PI+++F+ LD D    L   D
Sbjct: 288 PADLEAADLDDDGVVVAAEFILYKLKEMGKISQEDMTPIMEEFERLDFDKSGTLTTSD 345


>ref|XP_021978053.1| two-pore potassium channel 1-like [Helianthus annuus]
          Length = 365

 Score =  150 bits (378), Expect = 1e-40
 Identities = 82/178 (46%), Positives = 108/178 (60%), Gaps = 1/178 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           ++QTV   +  KR+E N  RNKC                 LV VEDLDF+HA YC++ TI
Sbjct: 169 MHQTVGTAETLKRMESNKVRNKCILLVVLLVVLMGAGTAVLVAVEDLDFVHAFYCVVGTI 228

Query: 376 IGVGS-DRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXX 200
             +G  D+CFST+GGRVFALFW+LSGT+YLG+LL+ F  L TQ R++   K A+K K   
Sbjct: 229 TSLGYIDKCFSTEGGRVFALFWILSGTIYLGQLLFAFAVLHTQSRQKSLVKWALKRK-TT 287

Query: 199 XXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                      DGV+ A+E+I++ L E GKISQEDM PI+++F+ LD D    L   D
Sbjct: 288 PADLEAADLDDDGVVVAAEFILYKLKEMGKISQEDMTPIMEEFERLDFDKTGTLTASD 345


>gb|KVH99181.1| EF-Hand 1, calcium-binding site-containing protein [Cynara
            cardunculus var. scolymus]
          Length = 1898

 Score =  147 bits (370), Expect = 2e-37
 Identities = 82/176 (46%), Positives = 108/176 (61%), Gaps = 1/176 (0%)
 Frame = -3

Query: 550  QTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATIIG 371
            QT+   +  KR++ N  RNKC               V L+ VEDLDFIHA YC++ATI  
Sbjct: 1333 QTIGTAETLKRMKTNQVRNKCILLVVLLVVLMAAGTVVLLSVEDLDFIHAFYCVVATITS 1392

Query: 370  VGS-DRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXXXX 194
            +G  D+CFST+GGRVFALFW+LSGT+YL +LL+ F  L TQ R+R   K A+K K     
Sbjct: 1393 LGYIDKCFSTRGGRVFALFWILSGTIYLAQLLFTFAVLHTQRRQRSLVKWALKRK-TTPA 1451

Query: 193  XXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                     DGV+ A+E+I++ L E GKISQEDM PI+++F+ LD D    L+  D
Sbjct: 1452 DLEAADLDDDGVVVAAEFILYKLKEMGKISQEDMTPIMEEFERLDFDKTGTLSASD 1507



 Score =  126 bits (317), Expect = 3e-30
 Identities = 72/137 (52%), Positives = 86/137 (62%)
 Frame = -3

Query: 436  LVRVEDLDFIHALYCILATIIGVGSDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELC 257
            LV +E+LD IHA YCILATI  VGSD CFSTKGGRVFA+FWML GT YL      F EL 
Sbjct: 1760 LVSLENLDNIHAFYCILATITSVGSDNCFSTKGGRVFAVFWMLLGTFYLALAFLTFAELY 1819

Query: 256  TQWRKRPSAKHAVKTKPXXXXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILK 77
            TQ RK+ S + +V  +              D V+  SEY +H L E GKISQEDMAPI++
Sbjct: 1820 TQ-RKQRSFEKSVLERTTTPEDFYAADFNSDRVLSISEYTLHKLSEMGKISQEDMAPIVE 1878

Query: 76   QFDTLDVDNMRKLALHD 26
             F  LDV++   L L +
Sbjct: 1879 DFRRLDVNDKGALTLRE 1895



 Score = 90.9 bits (224), Expect = 8e-18
 Identities = 63/160 (39%), Positives = 84/160 (52%), Gaps = 23/160 (14%)
 Frame = -3

Query: 436  LVRVEDLDFIHALYCILATIIGVGSDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELC 257
            LV +EDLDFIHALYCI  TI   G+++CFSTK GRVFAL  ML GTVY G +L+ FT++ 
Sbjct: 998  LVFIEDLDFIHALYCISITITSAGTEKCFSTKRGRVFALVLMLLGTVYKGYVLFTFTKVY 1057

Query: 256  TQ--WRKRPSAKHAVKTKP--XXXXXXXXXXXXXDGVIKA------SEYIIHIL------ 125
            TQ   R R   K+    +P                G+++A         + H +      
Sbjct: 1058 TQIKLRSREVKKNITPQEPEDSPPVKIDYDYDYDAGILRAEPVQLSDNEVDHDVEMAACP 1117

Query: 124  -----LETGKISQE--DMAPILKQFDTLDVDNMRKLALHD 26
                 +E G  SQ   DMA I+K+F  LDV+   +L LH+
Sbjct: 1118 QSQSPIEEGIPSQSEVDMADIMKEFRRLDVNGTGRLTLHE 1157



 Score = 83.6 bits (205), Expect = 3e-15
 Identities = 38/78 (48%), Positives = 52/78 (66%)
 Frame = -3

Query: 439 FLVRVEDLDFIHALYCILATIIGVGSDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTEL 260
           F+V +EDLDFIHALYC+  TI   G+++CFSTK GR+FAL WML G +Y G + + FTE+
Sbjct: 274 FVVLIEDLDFIHALYCVSITITSAGTEKCFSTKYGRIFALVWMLFGVMYKGYVFFIFTEV 333

Query: 259 CTQWRKRPSAKHAVKTKP 206
             + ++R        T P
Sbjct: 334 YIETKQRSEVLKRKITPP 351



 Score = 63.5 bits (153), Expect = 2e-08
 Identities = 42/122 (34%), Positives = 63/122 (51%), Gaps = 1/122 (0%)
 Frame = -3

Query: 436  LVRVEDLDFIHALYCILATIIGV-GSDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTEL 260
            L  + ++D   ALYC+ +TI  V  +++CFSTK  R+ AL W+L G + L  +LY F+E 
Sbjct: 697  LAFIGNMDLFRALYCVSSTITTVLSTNKCFSTKARRIIALIWILYGMITLSCVLYTFSEA 756

Query: 259  CTQWRKRPSAKHAVKTKPXXXXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPIL 80
             TQ  K    K  +KTK              DG I+  EY++H L E  +   +D    +
Sbjct: 757  WTQRSKWLFLK--MKTK-SGFQDLQPSDLDDDGFIRLEEYVLHKLKENKRSRGDDTETTI 813

Query: 79   KQ 74
            K+
Sbjct: 814  KE 815


>ref|XP_023740032.1| two-pore potassium channel 1-like [Lactuca sativa]
 ref|XP_023740033.1| two-pore potassium channel 1-like [Lactuca sativa]
 gb|PLY68977.1| hypothetical protein LSAT_9X90040 [Lactuca sativa]
          Length = 347

 Score =  134 bits (336), Expect = 1e-34
 Identities = 69/176 (39%), Positives = 105/176 (59%), Gaps = 1/176 (0%)
 Frame = -3

Query: 550 QTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATIIG 371
           +T+   ++ K+++    RNKC                  + VE+LDFIHALYC++AT+ G
Sbjct: 170 ETIGEAEILKKIKTKKVRNKCIILVVFLLVLMAAGTGIFIYVEELDFIHALYCVIATLTG 229

Query: 370 VGS-DRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXXXX 194
           +G  D+CFST GGRVFALFW+L GT+Y+ +LL+ F  L T+ R+R   K  +K K     
Sbjct: 230 LGYIDKCFSTTGGRVFALFWILLGTLYVAQLLFTFALLHTERRQRSLVKCVLKRK-TTGS 288

Query: 193 XXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                    +G++ A+E++I+ L E GKIS++D+ PI+ +F+TLD D    L+  D
Sbjct: 289 DLEAADFDCNGIVVAAEFVIYKLKEMGKISEDDITPIMDEFETLDFDKTGTLSASD 344


>ref|XP_023740005.1| two-pore potassium channel 1-like [Lactuca sativa]
 gb|PLY69001.1| hypothetical protein LSAT_9X90320 [Lactuca sativa]
          Length = 363

 Score =  127 bits (319), Expect = 6e-32
 Identities = 67/176 (38%), Positives = 105/176 (59%), Gaps = 1/176 (0%)
 Frame = -3

Query: 550 QTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATIIG 371
           +T+   ++ K+++  + R+KC                 L+ VEDL+FI+A YC++AT+ G
Sbjct: 169 ETIGEAEILKKIKTKSVRHKCITLLVFLLVFMAAGTGILLSVEDLNFINAFYCVVATLTG 228

Query: 370 VGS-DRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXXXX 194
           +G  D+CFST GGRVFALFW+L GT+Y+ +LL+ F  L T+ R+R   K  +K K     
Sbjct: 229 LGYIDKCFSTTGGRVFALFWILLGTLYVAQLLFTFALLHTERRQRSLVKWVLKRK-TTVS 287

Query: 193 XXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                    DG++ A+E+I+  L E GKI+++D+ PI+ +F+TLD D    L + D
Sbjct: 288 DLEAADFDGDGIVVAAEFIVFKLKEMGKINEDDITPIMDEFETLDFDKTGTLTVSD 343


>gb|KVI02335.1| EF-Hand 1, calcium-binding site-containing protein, partial [Cynara
           cardunculus var. scolymus]
          Length = 400

 Score =  122 bits (306), Expect = 9e-30
 Identities = 66/178 (37%), Positives = 97/178 (54%), Gaps = 1/178 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           L QT+   ++ K++     RNKC                FL+ VEDLDF+HA YC++ T+
Sbjct: 230 LNQTLGEREILKKIRTKKVRNKCIILVALLLVFMAAGTGFLLSVEDLDFVHAFYCVIGTL 289

Query: 376 IGVGS-DRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXX 200
            G+G  D+CFST GGRVF+LFW+L GT+Y+ +LL+ F  L T+ R+R             
Sbjct: 290 TGLGYIDKCFSTTGGRVFSLFWILLGTIYVAQLLFSFALLHTERRQR------------- 336

Query: 199 XXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                           A+E++I+ L E GKI++ED+ PI++QF+ LD D    L+  D
Sbjct: 337 ----------------AAEFVIYKLKEMGKINEEDITPIMEQFEILDFDKTGTLSTSD 378


>ref|XP_022001523.1| two-pore potassium channel 1-like [Helianthus annuus]
 gb|OTG01997.1| putative EF-hand domain pair, Potassium channel domain protein
           [Helianthus annuus]
          Length = 370

 Score =  119 bits (298), Expect = 8e-29
 Identities = 63/170 (37%), Positives = 100/170 (58%), Gaps = 1/170 (0%)
 Frame = -3

Query: 532 KLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATIIGVGS-DR 356
           ++ K+++    R KC                 L+ VEDL+FIHA YC++AT+ G+G    
Sbjct: 182 EILKKMKTKRVRYKCIILLLLLLVLMAAGTGLLLWVEDLEFIHAFYCVIATLTGLGYIHT 241

Query: 355 CFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXXXXXXXXXX 176
           CFST GGRVF++FW+L+GTVY+ +LL+ F  L T+ ++R   K  ++ K           
Sbjct: 242 CFSTTGGRVFSVFWILAGTVYVAQLLFTFALLYTERKQRSLVKSVLRRK-TTVSDLEAAD 300

Query: 175 XXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
              DG++ A+E+I++ L E GKI+++D+ PI+ +F+TLD D    L+  D
Sbjct: 301 FDGDGIVVAAEFIVYKLKEMGKITEDDVTPIMDEFETLDFDKTGTLSAAD 350


>gb|PLY93033.1| hypothetical protein LSAT_5X1461 [Lactuca sativa]
          Length = 293

 Score =  116 bits (290), Expect = 3e-28
 Identities = 61/117 (52%), Positives = 75/117 (64%), Gaps = 1/117 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           L QT+   +  K++E N  RNKC                 LV VEDLDFIHA YC++ATI
Sbjct: 159 LNQTLGTAETLKQMETNKVRNKCIILVALLVVLMAAGTTVLVAVEDLDFIHAFYCVVATI 218

Query: 376 IGVGS-DRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTK 209
             +G  D+CFSTKGGRVFALFW+LSGT+YLG+LL+ F  L TQ R+R   K A+K K
Sbjct: 219 TSLGYIDKCFSTKGGRVFALFWILSGTIYLGQLLFTFAMLHTQRRQRSLVKWALKRK 275


>gb|OTG19180.1| putative two pore domain potassium channel [Helianthus annuus]
          Length = 309

 Score =  115 bits (287), Expect = 1e-27
 Identities = 58/117 (49%), Positives = 75/117 (64%), Gaps = 1/117 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           ++QTV   +  KR+E N  RNKC                 LV VEDLDF+HA YC++ TI
Sbjct: 169 MHQTVGTAETLKRMESNKVRNKCILLVVLLVVLMGAGTAVLVAVEDLDFVHAFYCVVGTI 228

Query: 376 IGVGS-DRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTK 209
             +G  D+CFST+GGRVFALFW+LSGT+YLG+LL+ F  L TQ R++   K A+K K
Sbjct: 229 TSLGYIDKCFSTEGGRVFALFWILSGTIYLGQLLFAFAVLHTQSRQKSLVKWALKRK 285


>gb|PNT58681.1| hypothetical protein POPTR_001G366800v3 [Populus trichocarpa]
 gb|PNT58682.1| hypothetical protein POPTR_001G366800v3 [Populus trichocarpa]
          Length = 350

 Score =  114 bits (284), Expect = 6e-27
 Identities = 65/178 (36%), Positives = 100/178 (56%), Gaps = 1/178 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           ++Q +D     K +E N  + KC               +FL  VEDLD I A YC+ +T+
Sbjct: 164 MHQKLDPAAFLKEIETNKVKYKCYLAIIILSVLMLVGTIFLYMVEDLDIIDAFYCVCSTV 223

Query: 376 IGVG-SDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXX 200
             +G  D+ FST  GR+FA+FW+L+GT+ LG+L     EL T+ R+R +  + V T+   
Sbjct: 224 TTLGYGDKSFSTVYGRMFAVFWILTGTIALGQLFLYIAELFTESRQR-ALVNWVLTRRMT 282

Query: 199 XXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                      DGV+ A+E++++ L E GKIS+ED+A ++K+F+ LDVD    L+  D
Sbjct: 283 HLDLEAADIDDDGVVGAAEFVVYKLKEMGKISEEDIALVMKEFEDLDVDQSGTLSASD 340


>ref|XP_006370002.1| pulvinus outward-rectifying channel for potassium SPOCK1 family
           protein [Populus trichocarpa]
 ref|XP_002298862.2| hypothetical protein POPTR_0001s37550g [Populus trichocarpa]
          Length = 350

 Score =  114 bits (284), Expect = 6e-27
 Identities = 65/178 (36%), Positives = 100/178 (56%), Gaps = 1/178 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           ++Q +D     K +E N  + KC               +FL  VEDLD I A YC+ +T+
Sbjct: 164 MHQKLDPAAFLKEIETNKVKYKCYLAIIILSVLMLVGTIFLYMVEDLDIIDAFYCVCSTV 223

Query: 376 IGVG-SDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXX 200
             +G  D+ FST  GR+FA+FW+L+GT+ LG+L     EL T+ R+R +  + V T+   
Sbjct: 224 TTLGYGDKSFSTVYGRMFAVFWILTGTIALGQLFLYIAELFTESRQR-ALVNWVLTRRMT 282

Query: 199 XXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                      DGV+ A+E++++ L E GKIS+ED+A ++K+F+ LDVD    L+  D
Sbjct: 283 HLDLEAADIDDDGVVGAAEFVVYKLKEMGKISEEDIALVMKEFEDLDVDQSGTLSASD 340


>ref|XP_023524757.1| two-pore potassium channel 1-like [Cucurbita pepo subsp. pepo]
          Length = 359

 Score =  113 bits (283), Expect = 1e-26
 Identities = 67/183 (36%), Positives = 103/183 (56%), Gaps = 6/183 (3%)
 Frame = -3

Query: 556 LYQTVDATK-----LQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYC 392
           L++T++A +     + K ++ + ARNKC               VFLV +E L FI A YC
Sbjct: 171 LFKTLNAHQNGHCDMTKEIDTDKARNKCIVVFLLLILFIISGTVFLVNIEKLGFIDAFYC 230

Query: 391 ILATIIGVG-SDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVK 215
           + +TI  +G  D+ FSTK GR+FA+FW+L  T+ L +      EL T+ R++   K  V 
Sbjct: 231 VCSTITTLGYGDKSFSTKSGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKW-VL 289

Query: 214 TKPXXXXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLA 35
           +K              D V+ A+E++IH L E GKI+++D+AP+LK+F+ LDVD    L+
Sbjct: 290 SKKVTNLDLEAADLDNDRVVGAAEFVIHKLKEMGKITEDDIAPVLKEFEKLDVDQSGTLS 349

Query: 34  LHD 26
           + D
Sbjct: 350 ISD 352


>ref|XP_022939985.1| two-pore potassium channel 1-like [Cucurbita moschata]
          Length = 359

 Score =  113 bits (283), Expect = 1e-26
 Identities = 67/183 (36%), Positives = 103/183 (56%), Gaps = 6/183 (3%)
 Frame = -3

Query: 556 LYQTVDATK-----LQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYC 392
           L++T++A +     + K ++ + ARNKC               VFLV +E L FI A YC
Sbjct: 171 LFKTLNAHQNGHCDMTKEIDTDKARNKCIVVFLLLILFIISGTVFLVNIEKLGFIDAFYC 230

Query: 391 ILATIIGVG-SDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVK 215
           + +TI  +G  D+ FSTK GR+FA+FW+L  T+ L +      EL T+ R++   K  V 
Sbjct: 231 VCSTITTLGYGDKSFSTKSGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKW-VL 289

Query: 214 TKPXXXXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLA 35
           +K              D V+ A+E++IH L E GKI+++D+AP+LK+F+ LDVD    L+
Sbjct: 290 SKKVTNLDLEAADLDNDRVVGAAEFVIHKLKEMGKITEDDIAPVLKEFEKLDVDQSGTLS 349

Query: 34  LHD 26
           + D
Sbjct: 350 ISD 352


>gb|KZM95607.1| hypothetical protein DCAR_018849 [Daucus carota subsp. sativus]
          Length = 306

 Score =  112 bits (279), Expect = 2e-26
 Identities = 68/176 (38%), Positives = 91/176 (51%), Gaps = 1/176 (0%)
 Frame = -3

Query: 550 QTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATIIG 371
           Q V    + K +E N  R KC                FL  VE LD I A YC+ +TI  
Sbjct: 121 QKVGELDILKEIETNRVRYKCFMVLSALVVLIVAGTAFLATVEKLDLIDAFYCVCSTITT 180

Query: 370 VG-SDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXXXX 194
           +G  D+ FST  GR+FA+FW+L  T+ LG+L +   E+ T+ R+R   K  V  K     
Sbjct: 181 LGYGDKSFSTAAGRIFAIFWILMSTICLGQLFFCIAEVNTEIRQRALVK-LVLAKKVTNV 239

Query: 193 XXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                    DGV+ A+E+IIH L E GKI QED+  I++QF+ LDVD    L+  D
Sbjct: 240 DLEAADIDDDGVVGAAEFIIHRLKEMGKICQEDITAIMEQFEDLDVDQSGTLSPSD 295


>ref|XP_024199683.1| two-pore potassium channel 1-like [Rosa chinensis]
 ref|XP_024199684.1| two-pore potassium channel 1-like [Rosa chinensis]
 ref|XP_024199685.1| two-pore potassium channel 1-like [Rosa chinensis]
 ref|XP_024199686.1| two-pore potassium channel 1-like [Rosa chinensis]
 ref|XP_024199687.1| two-pore potassium channel 1-like [Rosa chinensis]
 ref|XP_024199688.1| two-pore potassium channel 1-like [Rosa chinensis]
 ref|XP_024199689.1| two-pore potassium channel 1-like [Rosa chinensis]
 gb|PRQ33108.1| putative Two pore domain potassium channel, EF-hand domain pair
           [Rosa chinensis]
          Length = 349

 Score =  112 bits (281), Expect = 2e-26
 Identities = 66/178 (37%), Positives = 98/178 (55%), Gaps = 1/178 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           ++Q    T + K VE N+ R KC               +FL  VE LD + A YC+ +TI
Sbjct: 164 VHQKFGQTDVLKEVETNSMRYKCITVFILLLVLMISGTIFLATVEKLDLVDAFYCVCSTI 223

Query: 376 IGVG-SDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXX 200
             +G  D+ FST+ GRVFA+FW+L+ T+ L + L    EL TQ ++R   K  V T+   
Sbjct: 224 TTLGYGDKSFSTEAGRVFAVFWILTSTICLAQFLLYIAELNTQHKQRALVKF-VLTRAMT 282

Query: 199 XXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                      DGV+ A+E++I+ L E GKI+QED+  ++++FD LDVD    L++ D
Sbjct: 283 NVDLEAADLDDDGVVGAAEFVIYKLKEMGKINQEDIRLVMEEFDALDVDQSGALSVSD 340


>ref|XP_017249114.1| PREDICTED: two-pore potassium channel 1-like [Daucus carota subsp.
           sativus]
 gb|KZM95603.1| hypothetical protein DCAR_018845 [Daucus carota subsp. sativus]
          Length = 353

 Score =  112 bits (281), Expect = 2e-26
 Identities = 68/176 (38%), Positives = 92/176 (52%), Gaps = 1/176 (0%)
 Frame = -3

Query: 550 QTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATIIG 371
           Q V    + K +E N  R KC                FL  VE LD I A YC+ +TI  
Sbjct: 168 QKVGELDILKEIETNRVRYKCFMVLSALVVLIVAGTAFLATVEKLDLIDAFYCVCSTITT 227

Query: 370 VG-SDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXXXX 194
           +G  D+ FST  GR+FA+FW+L+ T+ LG+L +   E+ T+ R+R   K  V  K     
Sbjct: 228 LGYGDKSFSTAAGRIFAIFWILTSTICLGQLFFCIAEVNTEIRQRALVK-LVLAKKVTNV 286

Query: 193 XXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                    DGV+ A+E+IIH L E GKI QED+  I++QF+ LDVD    L+  D
Sbjct: 287 DLEAADIDDDGVVGAAEFIIHRLKEMGKICQEDITAIMEQFEDLDVDQSGTLSPSD 342


>ref|XP_008462994.1| PREDICTED: two-pore potassium channel 1 isoform X1 [Cucumis melo]
          Length = 354

 Score =  112 bits (280), Expect = 3e-26
 Identities = 62/169 (36%), Positives = 94/169 (55%), Gaps = 1/169 (0%)
 Frame = -3

Query: 529 LQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATIIGVG-SDRC 353
           + K ++ N ARNKC                FLV +E LDFI A YC+ +TI  +G  D+ 
Sbjct: 180 ISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQS 239

Query: 352 FSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXXXXXXXXXXX 173
           FSTK GR+FA+FW+L  T+ L +      EL T+ R++   K  +  K            
Sbjct: 240 FSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSEK-VTDIDLEVADI 298

Query: 172 XXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
             DGV+ A+E++I+ L E GKI+++D++P+L +F+ LDVD    L++ D
Sbjct: 299 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISD 347


>ref|XP_011038782.1| PREDICTED: two-pore potassium channel 1 [Populus euphratica]
 ref|XP_011038783.1| PREDICTED: two-pore potassium channel 1 [Populus euphratica]
 ref|XP_011038784.1| PREDICTED: two-pore potassium channel 1 [Populus euphratica]
 ref|XP_011038786.1| PREDICTED: two-pore potassium channel 1 [Populus euphratica]
          Length = 353

 Score =  112 bits (279), Expect = 4e-26
 Identities = 65/178 (36%), Positives = 99/178 (55%), Gaps = 1/178 (0%)
 Frame = -3

Query: 556 LYQTVDATKLQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYCILATI 377
           ++Q +D     K +E N  + KC               +FL  VEDLD I A YC+ +T+
Sbjct: 167 MHQKLDPAAFHKEIETNKVKYKCYLAIIILSVLMLVGTIFLYMVEDLDIIDAFYCVCSTV 226

Query: 376 IGVG-SDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVKTKPXX 200
             +G  D+ FST  GR+FA+ W+L+GTV LG+L     EL T+ R+R +  + V T+   
Sbjct: 227 TTLGYGDKSFSTVYGRMFAVCWILTGTVALGQLFLYIAELFTESRQR-ALVNWVLTRRMT 285

Query: 199 XXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLALHD 26
                      DGV+ A+E++++ L E GKIS+ED+A ++K+F+ LDVD    L+  D
Sbjct: 286 NLDLEAADIDDDGVVGAAEFVVYKLKEMGKISEEDIALVMKEFEDLDVDQSGTLSASD 343


>ref|XP_022981526.1| two-pore potassium channel 1-like [Cucurbita maxima]
          Length = 355

 Score =  112 bits (279), Expect = 4e-26
 Identities = 67/183 (36%), Positives = 102/183 (55%), Gaps = 6/183 (3%)
 Frame = -3

Query: 556 LYQTVDATK-----LQKRVEFNTARNKCXXXXXXXXXXXXXXXVFLVRVEDLDFIHALYC 392
           L++T++A +     + K ++ + ARNKC               VFLV +E L FI A YC
Sbjct: 167 LFKTLNAHQNGHCDMTKEIDTDKARNKCIVVFLLLILFIISGTVFLVTIEKLGFIDAFYC 226

Query: 391 ILATIIGVG-SDRCFSTKGGRVFALFWMLSGTVYLGELLYRFTELCTQWRKRPSAKHAVK 215
           + +TI  +G  D+ FSTK GR+FA+FW+L  T+ L +      EL T+ R++   K  V 
Sbjct: 227 VCSTITTLGYGDKSFSTKSGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKW-VL 285

Query: 214 TKPXXXXXXXXXXXXXDGVIKASEYIIHILLETGKISQEDMAPILKQFDTLDVDNMRKLA 35
           +K              D V+ A+E++IH L E GKI+++D+AP+LK+F+ LDVD    L+
Sbjct: 286 SKKVTNLDLEAADLDNDRVVGAAEFVIHKLKEMGKITEDDIAPVLKEFEKLDVDQSGTLS 345

Query: 34  LHD 26
             D
Sbjct: 346 TSD 348