BLASTX nr result
ID: Chrysanthemum22_contig00036798
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Chrysanthemum22_contig00036798
(796 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_022014750.1| kinesin-like protein KIN-4A [Helianthus annuus] 221 2e-62
gb|OTF96141.1| putative kinesin motor domain-containing protein ... 221 2e-62
gb|PLY87298.1| hypothetical protein LSAT_4X164521 [Lactuca sativa] 201 2e-55
ref|XP_023761451.1| kinesin-like protein KIN-4A [Lactuca sativa] 201 3e-55
ref|XP_022026649.1| kinesin-like protein KIN-4A isoform X3 [Heli... 192 2e-52
ref|XP_022026646.1| kinesin-like protein KIN-4A isoform X1 [Heli... 192 3e-52
ref|XP_022026647.1| kinesin-like protein KIN-4A isoform X2 [Heli... 192 6e-52
gb|KVH92507.1| Kinesin, motor domain-containing protein [Cynara ... 190 3e-51
ref|XP_004291081.1| PREDICTED: chromosome-associated kinesin KIF... 106 4e-22
gb|KZV41647.1| hypothetical protein F511_25796 [Dorcoceras hygro... 103 3e-21
gb|PHU20769.1| Kinesin-like protein FRA1 [Capsicum chinense] 103 3e-21
gb|PHT30460.1| Kinesin-like protein FRA1 [Capsicum baccatum] 103 3e-21
ref|XP_016568787.1| PREDICTED: kinesin-like protein FRA1 [Capsic... 103 3e-21
ref|XP_009787508.1| PREDICTED: chromosome-associated kinesin KIF... 103 4e-21
ref|XP_009623113.1| PREDICTED: kinesin-like protein KIN-4A isofo... 103 4e-21
ref|XP_006354853.1| PREDICTED: kinesin-like protein FRA1 isoform... 103 4e-21
ref|XP_016474202.1| PREDICTED: kinesin-like protein FRA1 isoform... 103 4e-21
ref|XP_015073421.1| PREDICTED: kinesin-like protein FRA1 isoform... 103 4e-21
ref|XP_018632556.1| PREDICTED: kinesin-like protein KIN-4A isofo... 103 4e-21
ref|XP_009787507.1| PREDICTED: chromosome-associated kinesin KIF... 103 4e-21
>ref|XP_022014750.1| kinesin-like protein KIN-4A [Helianthus annuus]
Length = 990
Score = 221 bits (563), Expect = 2e-62
Identities = 102/123 (82%), Positives = 116/123 (94%), Gaps = 2/123 (1%)
Frame = -3
Query: 773 KGKAP--IDIFADLGLGSPLPFPESRELDTKAGICNRPLQTVFKQTQKMIPIAHMPMKKL 600
KG+A +DIFAD+GL SPLPFPESR+LD+KAGICNRPL+ FKQTQKMIP+AH+ MKKL
Sbjct: 868 KGRATTSLDIFADMGLESPLPFPESRQLDSKAGICNRPLKVAFKQTQKMIPMAHVSMKKL 927
Query: 599 PLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQIRNSDDTLKGQDLSMKLG 420
PLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLS+LI++SDQ+RNSDDTLKGQDLS++LG
Sbjct: 928 PLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSNLIKTSDQVRNSDDTLKGQDLSLRLG 987
Query: 419 PTT 411
PTT
Sbjct: 988 PTT 990
>gb|OTF96141.1| putative kinesin motor domain-containing protein [Helianthus annuus]
Length = 995
Score = 221 bits (563), Expect = 2e-62
Identities = 102/123 (82%), Positives = 116/123 (94%), Gaps = 2/123 (1%)
Frame = -3
Query: 773 KGKAP--IDIFADLGLGSPLPFPESRELDTKAGICNRPLQTVFKQTQKMIPIAHMPMKKL 600
KG+A +DIFAD+GL SPLPFPESR+LD+KAGICNRPL+ FKQTQKMIP+AH+ MKKL
Sbjct: 873 KGRATTSLDIFADMGLESPLPFPESRQLDSKAGICNRPLKVAFKQTQKMIPMAHVSMKKL 932
Query: 599 PLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQIRNSDDTLKGQDLSMKLG 420
PLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLS+LI++SDQ+RNSDDTLKGQDLS++LG
Sbjct: 933 PLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSNLIKTSDQVRNSDDTLKGQDLSLRLG 992
Query: 419 PTT 411
PTT
Sbjct: 993 PTT 995
>gb|PLY87298.1| hypothetical protein LSAT_4X164521 [Lactuca sativa]
Length = 1059
Score = 201 bits (512), Expect = 2e-55
Identities = 92/123 (74%), Positives = 106/123 (86%), Gaps = 2/123 (1%)
Frame = -3
Query: 773 KGKAP--IDIFADLGLGSPLPFPESRELDTKAGICNRPLQTVFKQTQKMIPIAHMPMKKL 600
KGKAP +DIFAD+G+G PLPFPES+E D+ AGICNRP++ ++KQT+KMIPIAH MKK
Sbjct: 937 KGKAPTTLDIFADMGIGPPLPFPESKESDSNAGICNRPVKVLYKQTEKMIPIAHTSMKKA 996
Query: 599 PLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQIRNSDDTLKGQDLSMKLG 420
PL EQKGK+SRWRR HDEWLIQFKWKWQKPWKL L S+DQIRNSD+TLKGQDLS +LG
Sbjct: 997 PLEEQKGKVSRWRRGHDEWLIQFKWKWQKPWKLGELFGSTDQIRNSDETLKGQDLSTRLG 1056
Query: 419 PTT 411
TT
Sbjct: 1057 QTT 1059
>ref|XP_023761451.1| kinesin-like protein KIN-4A [Lactuca sativa]
Length = 1099
Score = 201 bits (512), Expect = 3e-55
Identities = 92/123 (74%), Positives = 106/123 (86%), Gaps = 2/123 (1%)
Frame = -3
Query: 773 KGKAP--IDIFADLGLGSPLPFPESRELDTKAGICNRPLQTVFKQTQKMIPIAHMPMKKL 600
KGKAP +DIFAD+G+G PLPFPES+E D+ AGICNRP++ ++KQT+KMIPIAH MKK
Sbjct: 977 KGKAPTTLDIFADMGIGPPLPFPESKESDSNAGICNRPVKVLYKQTEKMIPIAHTSMKKA 1036
Query: 599 PLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQIRNSDDTLKGQDLSMKLG 420
PL EQKGK+SRWRR HDEWLIQFKWKWQKPWKL L S+DQIRNSD+TLKGQDLS +LG
Sbjct: 1037 PLEEQKGKVSRWRRGHDEWLIQFKWKWQKPWKLGELFGSTDQIRNSDETLKGQDLSTRLG 1096
Query: 419 PTT 411
TT
Sbjct: 1097 QTT 1099
>ref|XP_022026649.1| kinesin-like protein KIN-4A isoform X3 [Helianthus annuus]
Length = 958
Score = 192 bits (489), Expect = 2e-52
Identities = 89/117 (76%), Positives = 100/117 (85%)
Frame = -3
Query: 794 ETEGQAQKGKAPIDIFADLGLGSPLPFPESRELDTKAGICNRPLQTVFKQTQKMIPIAHM 615
ET + K P+DIFAD+G+ PLPFPESR+LD++AGICNRPL VFKQTQKMIP+AH+
Sbjct: 841 ETTTKQGKAPTPLDIFADMGIEYPLPFPESRQLDSEAGICNRPLPVVFKQTQKMIPMAHV 900
Query: 614 PMKKLPLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQIRNSDDTLKG 444
MKK PLGE GKISRW+RSHDEWLIQFKWKWQKPWKLS LIR+SDQIRNSD TLKG
Sbjct: 901 SMKKSPLGEHMGKISRWKRSHDEWLIQFKWKWQKPWKLSSLIRASDQIRNSDVTLKG 957
>ref|XP_022026646.1| kinesin-like protein KIN-4A isoform X1 [Helianthus annuus]
Length = 1057
Score = 192 bits (489), Expect = 3e-52
Identities = 89/117 (76%), Positives = 100/117 (85%)
Frame = -3
Query: 794 ETEGQAQKGKAPIDIFADLGLGSPLPFPESRELDTKAGICNRPLQTVFKQTQKMIPIAHM 615
ET + K P+DIFAD+G+ PLPFPESR+LD++AGICNRPL VFKQTQKMIP+AH+
Sbjct: 940 ETTTKQGKAPTPLDIFADMGIEYPLPFPESRQLDSEAGICNRPLPVVFKQTQKMIPMAHV 999
Query: 614 PMKKLPLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQIRNSDDTLKG 444
MKK PLGE GKISRW+RSHDEWLIQFKWKWQKPWKLS LIR+SDQIRNSD TLKG
Sbjct: 1000 SMKKSPLGEHMGKISRWKRSHDEWLIQFKWKWQKPWKLSSLIRASDQIRNSDVTLKG 1056
>ref|XP_022026647.1| kinesin-like protein KIN-4A isoform X2 [Helianthus annuus]
gb|OTG35615.1| putative kinesin motor domain-containing protein [Helianthus annuus]
Length = 1056
Score = 192 bits (487), Expect = 6e-52
Identities = 90/112 (80%), Positives = 100/112 (89%), Gaps = 2/112 (1%)
Frame = -3
Query: 773 KGKAP--IDIFADLGLGSPLPFPESRELDTKAGICNRPLQTVFKQTQKMIPIAHMPMKKL 600
KGKAP +DIFAD+G+ PLPFPESR+LD++AGICNRPL VFKQTQKMIP+AH+ MKK
Sbjct: 944 KGKAPTPLDIFADMGIEYPLPFPESRQLDSEAGICNRPLPVVFKQTQKMIPMAHVSMKKS 1003
Query: 599 PLGEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQIRNSDDTLKG 444
PLGE GKISRW+RSHDEWLIQFKWKWQKPWKLS LIR+SDQIRNSD TLKG
Sbjct: 1004 PLGEHMGKISRWKRSHDEWLIQFKWKWQKPWKLSSLIRASDQIRNSDVTLKG 1055
>gb|KVH92507.1| Kinesin, motor domain-containing protein [Cynara cardunculus var.
scolymus]
Length = 1099
Score = 190 bits (482), Expect = 3e-51
Identities = 91/121 (75%), Positives = 102/121 (84%)
Frame = -3
Query: 773 KGKAPIDIFADLGLGSPLPFPESRELDTKAGICNRPLQTVFKQTQKMIPIAHMPMKKLPL 594
K P DIFADLG+ SPLPFPESR+LD+KAGICNRP+Q +M+PIAH+ MKKLPL
Sbjct: 988 KAPTPFDIFADLGIESPLPFPESRQLDSKAGICNRPIQ-------EMVPIAHVCMKKLPL 1040
Query: 593 GEQKGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQIRNSDDTLKGQDLSMKLGPT 414
GEQKGK+SRWRRSHDEWLIQFKWKWQKPWKLS LIR D+IRNSD+TLK DLS+KL PT
Sbjct: 1041 GEQKGKVSRWRRSHDEWLIQFKWKWQKPWKLSQLIRIGDEIRNSDETLK--DLSIKLDPT 1098
Query: 413 T 411
T
Sbjct: 1099 T 1099
>ref|XP_004291081.1| PREDICTED: chromosome-associated kinesin KIF4A [Fragaria vesca subsp.
vesca]
Length = 1040
Score = 106 bits (264), Expect = 4e-22
Identities = 52/97 (53%), Positives = 67/97 (69%), Gaps = 5/97 (5%)
Frame = -3
Query: 749 FADLGLGS--PLPFPESRELDTKAGICNRPLQ--TVF-KQTQKMIPIAHMPMKKLPLGEQ 585
FAD GS P+ P ++L AGI N P++ T F QT+KM+PI H+P KKL + Q
Sbjct: 927 FADATNGSLSPISVPAQKQLKYTAGIANGPVRESTAFIDQTRKMVPIGHLPTKKLAIIGQ 986
Query: 584 KGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
GK+ RW+RSH +WL+QFKWKWQKPW+LS IR SD+
Sbjct: 987 SGKLWRWKRSHHQWLVQFKWKWQKPWRLSEWIRHSDE 1023
>gb|KZV41647.1| hypothetical protein F511_25796 [Dorcoceras hygrometricum]
Length = 1026
Score = 103 bits (257), Expect = 3e-21
Identities = 48/88 (54%), Positives = 60/88 (68%), Gaps = 3/88 (3%)
Frame = -3
Query: 728 SPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQKGKISRWRR 558
SP+P P ++L AGI N R QT+KM+PI + MKKL L Q GK+ RW+R
Sbjct: 918 SPIPVPAQKQLKYTAGIVNDTDRESAAFMDQTRKMVPIGQLSMKKLALVGQGGKLWRWKR 977
Query: 557 SHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
SH +WL+QFKWKWQKPW+LS LIR SD+
Sbjct: 978 SHHQWLLQFKWKWQKPWRLSELIRHSDE 1005
>gb|PHU20769.1| Kinesin-like protein FRA1 [Capsicum chinense]
Length = 1030
Score = 103 bits (257), Expect = 3e-21
Identities = 50/97 (51%), Positives = 63/97 (64%), Gaps = 5/97 (5%)
Frame = -3
Query: 749 FAD--LGLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQ 585
FAD G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q
Sbjct: 913 FADEMSGPPSPIPVPAQKQLKYSAGIANASVREAAAFIDQTRKMVPLGQLSMKKLTVAGQ 972
Query: 584 KGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
GK+ RW+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 973 GGKLWRWKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1009
>gb|PHT30460.1| Kinesin-like protein FRA1 [Capsicum baccatum]
Length = 1030
Score = 103 bits (257), Expect = 3e-21
Identities = 50/97 (51%), Positives = 63/97 (64%), Gaps = 5/97 (5%)
Frame = -3
Query: 749 FAD--LGLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQ 585
FAD G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q
Sbjct: 913 FADEMSGPPSPIPVPAQKQLKYSAGIANASVREAAAFIDQTRKMVPLGQLSMKKLTVAGQ 972
Query: 584 KGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
GK+ RW+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 973 GGKLWRWKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1009
>ref|XP_016568787.1| PREDICTED: kinesin-like protein FRA1 [Capsicum annuum]
gb|PHT63232.1| Kinesin-like protein FRA1 [Capsicum annuum]
Length = 1030
Score = 103 bits (257), Expect = 3e-21
Identities = 50/97 (51%), Positives = 63/97 (64%), Gaps = 5/97 (5%)
Frame = -3
Query: 749 FAD--LGLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQ 585
FAD G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q
Sbjct: 913 FADEMSGPPSPIPVPAQKQLKYSAGIANASVREAAAFIDQTRKMVPLGQLSMKKLTVAGQ 972
Query: 584 KGKISRWRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
GK+ RW+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 973 GGKLWRWKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1009
>ref|XP_009787508.1| PREDICTED: chromosome-associated kinesin KIF4A isoform X5 [Nicotiana
sylvestris]
Length = 1028
Score = 103 bits (256), Expect = 4e-21
Identities = 47/91 (51%), Positives = 60/91 (65%), Gaps = 3/91 (3%)
Frame = -3
Query: 737 GLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQKGKISR 567
G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q GK+ R
Sbjct: 917 GPPSPIPVPAQKQLKYSAGIANASVREAAAFIDQTRKMVPLGQLTMKKLTVAGQGGKLWR 976
Query: 566 WRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
W+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 977 WKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1007
>ref|XP_009623113.1| PREDICTED: kinesin-like protein KIN-4A isoform X4 [Nicotiana
tomentosiformis]
Length = 1028
Score = 103 bits (256), Expect = 4e-21
Identities = 47/91 (51%), Positives = 60/91 (65%), Gaps = 3/91 (3%)
Frame = -3
Query: 737 GLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQKGKISR 567
G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q GK+ R
Sbjct: 917 GPPSPIPVPAQKQLKYSAGIANASIREAAAFIDQTRKMVPLGQLSMKKLTVAGQGGKLWR 976
Query: 566 WRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
W+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 977 WKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1007
>ref|XP_006354853.1| PREDICTED: kinesin-like protein FRA1 isoform X2 [Solanum tuberosum]
Length = 1029
Score = 103 bits (256), Expect = 4e-21
Identities = 47/91 (51%), Positives = 60/91 (65%), Gaps = 3/91 (3%)
Frame = -3
Query: 737 GLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQKGKISR 567
G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q GK+ R
Sbjct: 918 GPPSPIPVPAQKQLKYSAGIANASVREAAAFMDQTRKMVPLGQLTMKKLTVAGQGGKLWR 977
Query: 566 WRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
W+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 978 WKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1008
>ref|XP_016474202.1| PREDICTED: kinesin-like protein FRA1 isoform X4 [Nicotiana tabacum]
Length = 1031
Score = 103 bits (256), Expect = 4e-21
Identities = 47/91 (51%), Positives = 60/91 (65%), Gaps = 3/91 (3%)
Frame = -3
Query: 737 GLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQKGKISR 567
G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q GK+ R
Sbjct: 920 GPPSPIPVPAQKQLKYSAGIANASIREAAAFIDQTRKMVPLGQLSMKKLTVAGQGGKLWR 979
Query: 566 WRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
W+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 980 WKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1010
>ref|XP_015073421.1| PREDICTED: kinesin-like protein FRA1 isoform X2 [Solanum pennellii]
Length = 1031
Score = 103 bits (256), Expect = 4e-21
Identities = 47/91 (51%), Positives = 60/91 (65%), Gaps = 3/91 (3%)
Frame = -3
Query: 737 GLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQKGKISR 567
G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q GK+ R
Sbjct: 920 GPPSPIPVPAQKQLKYSAGIANASVREAAAFMDQTRKMVPLGQLTMKKLTVAGQGGKLWR 979
Query: 566 WRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
W+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 980 WKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1010
>ref|XP_018632556.1| PREDICTED: kinesin-like protein KIN-4A isoform X3 [Nicotiana
tomentosiformis]
Length = 1032
Score = 103 bits (256), Expect = 4e-21
Identities = 47/91 (51%), Positives = 60/91 (65%), Gaps = 3/91 (3%)
Frame = -3
Query: 737 GLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQKGKISR 567
G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q GK+ R
Sbjct: 921 GPPSPIPVPAQKQLKYSAGIANASIREAAAFIDQTRKMVPLGQLSMKKLTVAGQGGKLWR 980
Query: 566 WRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
W+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 981 WKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1011
>ref|XP_009787507.1| PREDICTED: chromosome-associated kinesin KIF4 isoform X4 [Nicotiana
sylvestris]
Length = 1033
Score = 103 bits (256), Expect = 4e-21
Identities = 47/91 (51%), Positives = 60/91 (65%), Gaps = 3/91 (3%)
Frame = -3
Query: 737 GLGSPLPFPESRELDTKAGICN---RPLQTVFKQTQKMIPIAHMPMKKLPLGEQKGKISR 567
G SP+P P ++L AGI N R QT+KM+P+ + MKKL + Q GK+ R
Sbjct: 922 GPPSPIPVPAQKQLKYSAGIANASVREAAAFIDQTRKMVPLGQLTMKKLTVAGQGGKLWR 981
Query: 566 WRRSHDEWLIQFKWKWQKPWKLSHLIRSSDQ 474
W+RSH +WL+QFKWKWQKPWKLS IR SD+
Sbjct: 982 WKRSHHQWLLQFKWKWQKPWKLSEWIRHSDE 1012