BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00035343
         (737 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_021996607.1| probable ubiquitin-conjugating enzyme E2 37 ...    90   5e-17
gb|KVH93910.1| Ubiquitin-conjugating enzyme, active site-contain...    73   5e-11

>ref|XP_021996607.1| probable ubiquitin-conjugating enzyme E2 37 [Helianthus annuus]
 gb|OTG03819.1| putative ubiquitin-conjugating enzyme/RWD-like protein [Helianthus
           annuus]
          Length = 456

 Score = 90.1 bits (222), Expect = 5e-17
 Identities = 60/146 (41%), Positives = 78/146 (53%), Gaps = 31/146 (21%)
 Frame = -1

Query: 401 ESDFHDYAVGCKRLSGIGRKLSLDAS--GSNTRDRDMGLKKASSNE-------------- 270
           ++D  +YA   K+LSG GRKLSLDAS  GSN R+RD G+ + +SN               
Sbjct: 191 KTDLREYAASFKKLSGTGRKLSLDASYSGSNARERDTGMNEITSNTVRQSPIQEPKEGSK 250

Query: 269 --SLECSKDHVMNVKNSDH-------------DRHTKKPTISSKKLRLSNKNLQKEDPDA 135
             S E  KDHV+   NSDH             D+  ++P ISSKKL LS+KN+Q      
Sbjct: 251 DTSFEYGKDHVLTAMNSDHVQYNKPPVISSDRDQCNERPVISSKKLCLSSKNVQHGKDHV 310

Query: 134 TKRHDDVMSRDLGAQKENLLEVQKQT 57
               D  +  DL AQKENLL+ QK++
Sbjct: 311 L---DSTVVHDLDAQKENLLKDQKKS 333



 Score = 79.3 bits (194), Expect = 3e-13
 Identities = 43/75 (57%), Positives = 51/75 (68%), Gaps = 5/75 (6%)
 Frame = -1

Query: 737 LSGIGRKLSLDAT--GSNTRDRDTGMNKAPLN---ESQIQEPNQDFKEMPFEYCKSQVMT 573
           LSG GRKLSLDA+  GSN R+RDTGMN+   N   +S IQEP +  K+  FEY K  V+T
Sbjct: 204 LSGTGRKLSLDASYSGSNARERDTGMNEITSNTVRQSPIQEPKEGSKDTSFEYGKDHVLT 263

Query: 572 MMNSDHDQGTKPPAV 528
            MNSDH Q  KPP +
Sbjct: 264 AMNSDHVQYNKPPVI 278


>gb|KVH93910.1| Ubiquitin-conjugating enzyme, active site-containing protein
           [Cynara cardunculus var. scolymus]
          Length = 488

 Score = 72.8 bits (177), Expect = 5e-11
 Identities = 56/142 (39%), Positives = 73/142 (51%), Gaps = 30/142 (21%)
 Frame = -1

Query: 395 DFHDYAVGCKRLSGIGRKLSLDASGSNTRDRDMGLKKASSN---------ESLECSKD-- 249
           D H+YAV  K+LSG  RKLSLDASGSNT++ D  + +A SN         E  + SKD  
Sbjct: 202 DTHEYAVDLKKLSGTNRKLSLDASGSNTKEVDTRMNEAPSNTIHGQSQIQERKQDSKDTQ 261

Query: 248 --HVMNVKN-----------------SDHDRHTKKPTISSKKLRLSNKNLQKEDPDATKR 126
             +VMN ++                  DH +  K   +SS KL LS KN Q E   + KR
Sbjct: 262 FKYVMNPQSIPLISSTNHYDQFPDQPLDHHQDHKPTVVSSTKLCLSGKNTQAEILASIKR 321

Query: 125 HDDVMSRDLGAQKENLLEVQKQ 60
            DD +S  L A   N L++QK+
Sbjct: 322 TDDPISSVLSA--HNDLKIQKE 341



 Score = 62.0 bits (149), Expect = 2e-07
 Identities = 43/112 (38%), Positives = 57/112 (50%), Gaps = 17/112 (15%)
 Frame = -1

Query: 737 LSGIGRKLSLDATGSNTRDRDTGMNKAPLN----ESQIQEPNQDFKEMPFEYCKS-QVMT 573
           LSG  RKLSLDA+GSNT++ DT MN+AP N    +SQIQE  QD K+  F+Y  + Q + 
Sbjct: 213 LSGTNRKLSLDASGSNTKEVDTRMNEAPSNTIHGQSQIQERKQDSKDTQFKYVMNPQSIP 272

Query: 572 MMNS------------DHDQGTKPPAVXXXXXXXXXXXXXKEVPDSTNRLED 453
           +++S            DH Q  KP  V              E+  S  R +D
Sbjct: 273 LISSTNHYDQFPDQPLDHHQDHKPTVVSSTKLCLSGKNTQAEILASIKRTDD 324