BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00031858
         (1639 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_021982081.1| cytoplasmic tRNA 2-thiolation protein 2 isof...   719   0.0  
ref|XP_021982080.1| cytoplasmic tRNA 2-thiolation protein 2 isof...   715   0.0  
ref|XP_023737631.1| cytoplasmic tRNA 2-thiolation protein 2 [Lac...   689   0.0  
gb|KVI12121.1| Thiouridylase, cytoplasmic, subunit 2 [Cynara car...   635   0.0  
ref|XP_018805161.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   553   0.0  
ref|XP_015898174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   555   0.0  
ref|XP_018841765.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   551   0.0  
ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   550   0.0  
ref|XP_015898173.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   548   0.0  
ref|XP_017238071.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   545   0.0  
ref|XP_006351313.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   546   0.0  
ref|XP_023920473.1| cytoplasmic tRNA 2-thiolation protein 2 [Que...   540   0.0  
gb|POF00280.1| cytoplasmic trna 2-thiolation protein 2 [Quercus ...   540   0.0  
ref|XP_004249262.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   538   0.0  
ref|XP_015089096.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   537   0.0  
ref|XP_006421388.1| cytoplasmic tRNA 2-thiolation protein 2 isof...   536   0.0  
ref|XP_019224820.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   536   0.0  
ref|XP_007028730.2| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   535   0.0  
gb|EOY09232.1| Adenine nucleotide alpha hydrolases-like superfam...   534   0.0  
gb|KZN00307.1| hypothetical protein DCAR_009061 [Daucus carota s...   534   0.0  

>ref|XP_021982081.1| cytoplasmic tRNA 2-thiolation protein 2 isoform X2 [Helianthus
            annuus]
 gb|OTG14720.1| putative adenine nucleotide alpha hydrolases-like superfamily protein
            [Helianthus annuus]
          Length = 455

 Score =  719 bits (1857), Expect = 0.0
 Identities = 347/412 (84%), Positives = 381/412 (92%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            CLKCK++EAT+FTGG+GGGRFCADCFRSNLYGKFKLAVTSNAMISP DKVLVAFSGG SS
Sbjct: 44   CLKCKATEATVFTGGDGGGRFCADCFRSNLYGKFKLAVTSNAMISPTDKVLVAFSGGPSS 103

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RVALQFVSEMQLKAQKN+DATANRDRSLPVF VGVAFIDESE+HP+AS   DKA+EDIK 
Sbjct: 104  RVALQFVSEMQLKAQKNYDATANRDRSLPVFAVGVAFIDESEIHPVASAAFDKAVEDIKE 163

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV NL  PTKELHV PI  +YSTKT+DGK+ML+NL N+V+DATGKEDLLTHLRM+SLQK+
Sbjct: 164  IVSNLPLPTKELHVAPISHVYSTKTSDGKQMLRNLTNSVSDATGKEDLLTHLRMLSLQKV 223

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A +NGYTKLVLGSCTSRIACHVL+ATVKGQGYSLAADIQYADARWEVPVVLPLRDC +QE
Sbjct: 224  AIKNGYTKLVLGSCTSRIACHVLSATVKGQGYSLAADIQYADARWEVPVVLPLRDCPLQE 283

Query: 742  INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 563
            +NILCNLDSLKIV++FN++ AGINGL+SSFVK+LQEENP+RECTIVRTAGKLTPFPFNRI
Sbjct: 284  LNILCNLDSLKIVDIFNETGAGINGLVSSFVKILQEENPSRECTIVRTAGKLTPFPFNRI 343

Query: 562  PEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPMSCYKNPQPLDNIFGATCCSS 383
            PEANVDD DL SQRRQK+FNLKPIESLPPE+FCPICNSPMSCY N +    +F A CC S
Sbjct: 344  PEANVDDADLVSQRRQKKFNLKPIESLPPESFCPICNSPMSCYGNAESRPELFRAACCPS 403

Query: 382  CQYQILPEEPSSMDQFYSFLPESIISRAKNGNEKWIREKIQDCLLSDGEDEV 227
            CQYQILP+E SSMDQFYSFLPESII+RAKNGN+KWIREKIQDCLL D EDEV
Sbjct: 404  CQYQILPDEASSMDQFYSFLPESIIARAKNGNQKWIREKIQDCLLLDDEDEV 455


>ref|XP_021982080.1| cytoplasmic tRNA 2-thiolation protein 2 isoform X1 [Helianthus
            annuus]
          Length = 456

 Score =  715 bits (1845), Expect = 0.0
 Identities = 347/413 (84%), Positives = 381/413 (92%), Gaps = 1/413 (0%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            CLKCK++EAT+FTGG+GGGRFCADCFRSNLYGKFKLAVTSNAMISP DKVLVAFSGG SS
Sbjct: 44   CLKCKATEATVFTGGDGGGRFCADCFRSNLYGKFKLAVTSNAMISPTDKVLVAFSGGPSS 103

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RVALQFVSEMQLKAQKN+DATANRDRSLPVF VGVAFIDESE+HP+AS   DKA+EDIK 
Sbjct: 104  RVALQFVSEMQLKAQKNYDATANRDRSLPVFAVGVAFIDESEIHPVASAAFDKAVEDIKE 163

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV NL  PTKELHV PI  +YSTKT+DGK+ML+NL N+V+DATGKEDLLTHLRM+SLQK+
Sbjct: 164  IVSNLPLPTKELHVAPISHVYSTKTSDGKQMLRNLTNSVSDATGKEDLLTHLRMLSLQKV 223

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A +NGYTKLVLGSCTSRIACHVL+ATVKGQGYSLAADIQYADARWEVPVVLPLRDC +QE
Sbjct: 224  AIKNGYTKLVLGSCTSRIACHVLSATVKGQGYSLAADIQYADARWEVPVVLPLRDCPLQE 283

Query: 742  INILCNLD-SLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNR 566
            +NILCNLD SLKIV++FN++ AGINGL+SSFVK+LQEENP+RECTIVRTAGKLTPFPFNR
Sbjct: 284  LNILCNLDSSLKIVDIFNETGAGINGLVSSFVKILQEENPSRECTIVRTAGKLTPFPFNR 343

Query: 565  IPEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPMSCYKNPQPLDNIFGATCCS 386
            IPEANVDD DL SQRRQK+FNLKPIESLPPE+FCPICNSPMSCY N +    +F A CC 
Sbjct: 344  IPEANVDDADLVSQRRQKKFNLKPIESLPPESFCPICNSPMSCYGNAESRPELFRAACCP 403

Query: 385  SCQYQILPEEPSSMDQFYSFLPESIISRAKNGNEKWIREKIQDCLLSDGEDEV 227
            SCQYQILP+E SSMDQFYSFLPESII+RAKNGN+KWIREKIQDCLL D EDEV
Sbjct: 404  SCQYQILPDEASSMDQFYSFLPESIIARAKNGNQKWIREKIQDCLLLDDEDEV 456


>ref|XP_023737631.1| cytoplasmic tRNA 2-thiolation protein 2 [Lactuca sativa]
 gb|PLY70842.1| hypothetical protein LSAT_8X81281 [Lactuca sativa]
          Length = 465

 Score =  689 bits (1778), Expect = 0.0
 Identities = 340/420 (80%), Positives = 377/420 (89%), Gaps = 8/420 (1%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            CLKCK++EATIFTGGEGG RFCADCFRSNLYGKFKLAVTS+AMISP DKVLVAFSGG SS
Sbjct: 46   CLKCKAAEATIFTGGEGGSRFCADCFRSNLYGKFKLAVTSHAMISPTDKVLVAFSGGPSS 105

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RVALQFVSEMQLK+QKNFDATA RDRSLPVFGVGVAFIDE+E+HPI   D +KAIEDIK 
Sbjct: 106  RVALQFVSEMQLKSQKNFDATAIRDRSLPVFGVGVAFIDETELHPITCTDFNKAIEDIKE 165

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV NL+P  KELH+VPIR+IYSTKT+DG E L+NLINTV+DATGKEDLL+HLRM+SLQKI
Sbjct: 166  IVSNLSPTKKELHIVPIRNIYSTKTSDGSETLKNLINTVSDATGKEDLLSHLRMLSLQKI 225

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A ENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWE+PVVLPLRDCL+QE
Sbjct: 226  AIENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEIPVVLPLRDCLIQE 285

Query: 742  INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 563
            +NILC+LDSLKIVE F DS+ GINGL+SSFVK+LQEENP+RECTIVRTAGKLTPFPFN+I
Sbjct: 286  LNILCSLDSLKIVEEFKDSRGGINGLVSSFVKLLQEENPSRECTIVRTAGKLTPFPFNKI 345

Query: 562  PEANVDDTD--LASQRRQKRFNLKPIESLPPEAFCPICNSP------MSCYKNPQPLDNI 407
            PE N D  D  LASQRR K+FNLKPIESLPP++FCPICNSP      ++ + N Q    +
Sbjct: 346  PETNTDSNDVHLASQRRHKKFNLKPIESLPPDSFCPICNSPIRDLQSVTSFTNSQTTPEL 405

Query: 406  FGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNGNEKWIREKIQDCLLSDGEDEV 227
            FGA CCSSCQYQILPEE SSM++FYSFLP+SII RA+NGN+KWIREKIQDCL+ D EDEV
Sbjct: 406  FGAACCSSCQYQILPEELSSMEEFYSFLPQSIIERARNGNQKWIREKIQDCLILDDEDEV 465


>gb|KVI12121.1| Thiouridylase, cytoplasmic, subunit 2 [Cynara cardunculus var.
            scolymus]
          Length = 447

 Score =  635 bits (1638), Expect = 0.0
 Identities = 322/426 (75%), Positives = 350/426 (82%), Gaps = 29/426 (6%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            CLKCK +EATIFTGG+GGGRFCADCFRSNLYGKFKLAVTSNAMISP DKVLVAFSGG SS
Sbjct: 43   CLKCKGTEATIFTGGDGGGRFCADCFRSNLYGKFKLAVTSNAMISPTDKVLVAFSGGPSS 102

Query: 1282 R-----------------------VALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAF 1172
                                    VALQFVSEMQLKAQKNFDATA+RDRSLPVFGVGVAF
Sbjct: 103  SCSDLSQANSLDQVLLDTFKDLNVVALQFVSEMQLKAQKNFDATADRDRSLPVFGVGVAF 162

Query: 1171 IDESEVHPIASGDLDKAIEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLIN 992
            IDESE+HP  + DLDKA+ED+K IVLNLAPP KELHVVPIRSI S KT+DG+E L+NL+N
Sbjct: 163  IDESEIHPSTAADLDKAVEDMKEIVLNLAPPRKELHVVPIRSICSEKTSDGRETLENLVN 222

Query: 991  TVTDATGKEDLLTHLRMMSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAAD 812
                                  IA ENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAAD
Sbjct: 223  A---------------------IAIENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAAD 261

Query: 811  IQYADARWEVPVVLPLRDCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEE 632
            IQYADARWE+PVVLPLRDCLMQE+NILC+LDSLKIVEVFNDS+A INGL+SSFVK+LQEE
Sbjct: 262  IQYADARWEIPVVLPLRDCLMQELNILCSLDSLKIVEVFNDSRANINGLVSSFVKLLQEE 321

Query: 631  NPARECTIVRTAGKLTPFPFNRIPEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICN 452
            NP+RECTIVRTAGKLTPFPFNRIPE + DD  LASQRRQK+FNL+PIESLPPE+FCPICN
Sbjct: 322  NPSRECTIVRTAGKLTPFPFNRIPEVDADDVLLASQRRQKKFNLRPIESLPPESFCPICN 381

Query: 451  SPM------SCYKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG 290
            SPM      SCY NPQP   +F A CCS CQYQ+LPEEPSSMDQFYSFLP+SII+RAK+G
Sbjct: 382  SPMNDLQNSSCYSNPQPNPELFRAACCSCCQYQVLPEEPSSMDQFYSFLPKSIITRAKDG 441

Query: 289  NEKWIR 272
            N+KWIR
Sbjct: 442  NQKWIR 447


>ref|XP_018805161.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Juglans
            regia]
          Length = 461

 Score =  553 bits (1425), Expect = 0.0
 Identities = 276/422 (65%), Positives = 339/422 (80%), Gaps = 11/422 (2%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            C+KCKS+E  I  G    GRFCADCFRSNLYGKF+LAV SNAMI P D VLVAFSGG+SS
Sbjct: 42   CVKCKSNEP-ISGGNAEDGRFCADCFRSNLYGKFRLAVISNAMICPTDNVLVAFSGGSSS 100

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RVALQFV EMQ K+QKNFDA+  RDRSLPVFGVGVAFIDE  + P++S ++DKAI+D + 
Sbjct: 101  RVALQFVHEMQDKSQKNFDAS--RDRSLPVFGVGVAFIDERAIFPVSSHEIDKAIQDFRS 158

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV  LAPP KELHVVPI +I S+ ++DG++ L+NL++ ++D+TGKEDLL HLRM+SLQKI
Sbjct: 159  IVSALAPPRKELHVVPIENICSSSSSDGRDRLKNLLDAISDSTGKEDLLLHLRMLSLQKI 218

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A+ENGY ++VLGSCTS IACHV++A VKGQGYSL  DIQY DARWE+PVVLPLRDCL QE
Sbjct: 219  ASENGYNRVVLGSCTSSIACHVISAIVKGQGYSLPGDIQYVDARWEIPVVLPLRDCLTQE 278

Query: 742  INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 563
            +N LC+LD LK VE+     +GINGL+SSFV +LQ+ENP+RECTIVRTAGKLTPF FN I
Sbjct: 279  LNRLCHLDGLKTVELHKVPYSGINGLVSSFVTLLQKENPSRECTIVRTAGKLTPFHFNSI 338

Query: 562  PEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPM--------SCYKNPQPLDNI 407
            PE +  +T LA+QRR K++NLKP ESL  E+FCP+CNSP+        S  +N Q   +I
Sbjct: 339  PEKSDSNTTLATQRRMKKYNLKPNESLSSESFCPVCNSPLNKSDLLRQSNLQNHQTSSDI 398

Query: 406  FGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKN---GNEKWIREKIQDCLLSDGE 236
            FGA CCSSC++QILP++PSSM+ FY+ LP+ +++RAK    GN   +RE+IQDCL+SD E
Sbjct: 399  FGAACCSSCRFQILPKDPSSMEHFYTHLPQPLVARAKRGGYGNLSLLREQIQDCLISDSE 458

Query: 235  DE 230
            DE
Sbjct: 459  DE 460


>ref|XP_015898174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X2
            [Ziziphus jujuba]
          Length = 505

 Score =  555 bits (1429), Expect = 0.0
 Identities = 279/424 (65%), Positives = 337/424 (79%), Gaps = 13/424 (3%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGG--EGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGA 1289
            C+KCK+++   F G   +   RFC DCFRSNL+GKF+LAVTSNAMI P D VLVAFSGGA
Sbjct: 83   CIKCKANQPMSFAGRRTDDDARFCGDCFRSNLFGKFRLAVTSNAMICPTDNVLVAFSGGA 142

Query: 1288 SSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDI 1109
            SSRVALQFV EMQ KAQKNFDA+  RDRSLPVFGVGVAFIDES +  +   ++ K IEDI
Sbjct: 143  SSRVALQFVHEMQHKAQKNFDAS--RDRSLPVFGVGVAFIDESSIFTVPEDEVTKGIEDI 200

Query: 1108 KGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQ 929
            + IV +L PPTKELHVVPI S+YS+ +NDG + L+ L++ V DATGKEDLL +LRM+SLQ
Sbjct: 201  RLIVSSLTPPTKELHVVPIESLYSSDSNDGSDRLKKLLDAVCDATGKEDLLVYLRMLSLQ 260

Query: 928  KIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLM 749
            KIA+ENGY +LVLGSCTSRIACHVL ATVKG GYSL ADIQY D+RW VPVVLPLRDCL+
Sbjct: 261  KIASENGYNRLVLGSCTSRIACHVLTATVKGHGYSLPADIQYVDSRWGVPVVLPLRDCLI 320

Query: 748  QEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFN 569
            QE+N+LC+LD LK VE+  +  + INGL+SSFV +LQEENP+RECTIVRTAGKLTPF FN
Sbjct: 321  QELNMLCHLDGLKTVELNKNPSSSINGLVSSFVTLLQEENPSRECTIVRTAGKLTPFHFN 380

Query: 568  RIPEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPMS-----CYKNPQPLDN-- 410
            RIPE N  +  LA+QRRQKR+NLKP ES+  E+FCPICNSP+S      +KN +  D   
Sbjct: 381  RIPEINDSNVPLATQRRQKRYNLKPTESISSESFCPICNSPLSKSDLLSFKNVESCDTNS 440

Query: 409  -IFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG---NEKWIREKIQDCLLSD 242
             +F A CCSSC++QILP++PSS+  FYS LP+ +++RAK G   N   +RE+I+DC+LSD
Sbjct: 441  VLFSAACCSSCRFQILPKDPSSVGDFYSLLPQPVVARAKCGSADNYSLLREQIKDCILSD 500

Query: 241  GEDE 230
             EDE
Sbjct: 501  SEDE 504


>ref|XP_018841765.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Juglans
            regia]
          Length = 461

 Score =  551 bits (1420), Expect = 0.0
 Identities = 276/422 (65%), Positives = 338/422 (80%), Gaps = 11/422 (2%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            C+KCKS+E  I  G    GRFCADCFRSNLYGKF+LAV SNAMI P D VLVAFSGG SS
Sbjct: 42   CVKCKSNEP-ISGGNAEDGRFCADCFRSNLYGKFRLAVISNAMICPTDNVLVAFSGGPSS 100

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RVALQFV EMQ K+QKNFDA+  RDRSLPVFGVGVAFIDE  + P+ S ++DKAI+D + 
Sbjct: 101  RVALQFVHEMQDKSQKNFDAS--RDRSLPVFGVGVAFIDERAIFPVPSHEIDKAIQDFRS 158

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV  LAPP KELHVVPI +I S+ ++DG++ L+NL++ ++D+TGKEDLL HLRM+SLQKI
Sbjct: 159  IVSALAPPRKELHVVPIENICSSSSSDGRDRLKNLLDAISDSTGKEDLLLHLRMLSLQKI 218

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A+ENGY ++VLGSCTS IACHV++A VKGQGYSL  DIQY DARWE+PVVLPLRDCL QE
Sbjct: 219  ASENGYNRVVLGSCTSSIACHVISAIVKGQGYSLPGDIQYVDARWEIPVVLPLRDCLTQE 278

Query: 742  INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 563
            +N LC+LD LK VE+     +GINGL+SSFV +LQ+ENP+RECTIVRTAGKLTPF FN I
Sbjct: 279  LNRLCHLDGLKTVELHKVPYSGINGLVSSFVTLLQKENPSRECTIVRTAGKLTPFHFNSI 338

Query: 562  PEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPM--------SCYKNPQPLDNI 407
            PE +  +T LA+QRR K++NLKP ESL  E+FCP+CNSP+        S  +N Q   +I
Sbjct: 339  PEKSDSNTTLATQRRLKKYNLKPNESLSSESFCPVCNSPLNKSDLLRQSNLQNHQTSSDI 398

Query: 406  FGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKN---GNEKWIREKIQDCLLSDGE 236
            FGA CCSSC++QILP++PSSM+ FY+ LP+ +++RAK+   GN   +RE+IQDCL+SD E
Sbjct: 399  FGAACCSSCRFQILPKDPSSMEHFYTHLPQPLVARAKHSGYGNLSLLREQIQDCLISDSE 458

Query: 235  DE 230
            DE
Sbjct: 459  DE 460


>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
 emb|CBI29838.3| unnamed protein product, partial [Vitis vinifera]
          Length = 468

 Score =  550 bits (1417), Expect = 0.0
 Identities = 283/425 (66%), Positives = 336/425 (79%), Gaps = 15/425 (3%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGG---EGG-GRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSG 1295
            CLKCK +E    +G    +G  GRFC DCFR NL+GKFKLAVTSNAMISP D VLVAFSG
Sbjct: 47   CLKCKVNETLASSGTVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSG 106

Query: 1294 GASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIE 1115
            G SSRVALQFV EMQ KAQKNFDA+  RDRSLPVFGVGVAF+DES V  I S + DKAI+
Sbjct: 107  GPSSRVALQFVHEMQHKAQKNFDAS--RDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQ 164

Query: 1114 DIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMS 935
            D+K IV NLAPP K  HVVPI S+YS+   +G++ L+ L+N V D TGKEDLL HLRM+S
Sbjct: 165  DMKSIVSNLAPPDKAFHVVPIESVYSS---NGRDRLEELLNAVKDVTGKEDLLQHLRMLS 221

Query: 934  LQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDC 755
            LQK A ENGYTKL+LGSCTSRIACHV++ATVKGQGYSL ADIQY DARWE+PVVLPLRDC
Sbjct: 222  LQKNAYENGYTKLLLGSCTSRIACHVISATVKGQGYSLPADIQYVDARWEIPVVLPLRDC 281

Query: 754  LMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFP 575
            L QE+N+LC LD LK +EV N   +GINGL++SFV +LQEENP+RECTIVRTAGKLTPF 
Sbjct: 282  LAQELNMLCRLDGLKTMEVLNSPHSGINGLVASFVTLLQEENPSRECTIVRTAGKLTPFH 341

Query: 574  FNRIPEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSP--------MSCYKNPQP 419
            FN+IPE++  +  LAS+RRQK+FNLKP ES+PPE++CPICNSP        +S   + Q 
Sbjct: 342  FNQIPESDDSNVHLASRRRQKKFNLKPNESVPPESYCPICNSPLSKSDHINLSSLGSDQT 401

Query: 418  LDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNGN---EKWIREKIQDCLL 248
              +IFGATCC SCQ+QILP+EP SM+ FYS LP+  + +AK+ +   ++ +RE+IQD LL
Sbjct: 402  NADIFGATCCPSCQFQILPKEPVSMEHFYSLLPQQFVVQAKDRSFSMQRQLREQIQDFLL 461

Query: 247  SDGED 233
            SD ED
Sbjct: 462  SDSED 466


>ref|XP_015898173.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X1
            [Ziziphus jujuba]
          Length = 512

 Score =  548 bits (1411), Expect = 0.0
 Identities = 279/431 (64%), Positives = 337/431 (78%), Gaps = 20/431 (4%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGG--EGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGA 1289
            C+KCK+++   F G   +   RFC DCFRSNL+GKF+LAVTSNAMI P D VLVAFSGGA
Sbjct: 83   CIKCKANQPMSFAGRRTDDDARFCGDCFRSNLFGKFRLAVTSNAMICPTDNVLVAFSGGA 142

Query: 1288 SSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDI 1109
            SSRVALQFV EMQ KAQKNFDA+  RDRSLPVFGVGVAFIDES +  +   ++ K IEDI
Sbjct: 143  SSRVALQFVHEMQHKAQKNFDAS--RDRSLPVFGVGVAFIDESSIFTVPEDEVTKGIEDI 200

Query: 1108 KGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQ 929
            + IV +L PPTKELHVVPI S+YS+ +NDG + L+ L++ V DATGKEDLL +LRM+SLQ
Sbjct: 201  RLIVSSLTPPTKELHVVPIESLYSSDSNDGSDRLKKLLDAVCDATGKEDLLVYLRMLSLQ 260

Query: 928  KIATENGYTKLVLGSCTSRIACHVLAATVK-------GQGYSLAADIQYADARWEVPVVL 770
            KIA+ENGY +LVLGSCTSRIACHVL ATVK       G GYSL ADIQY D+RW VPVVL
Sbjct: 261  KIASENGYNRLVLGSCTSRIACHVLTATVKVRTFFQQGHGYSLPADIQYVDSRWGVPVVL 320

Query: 769  PLRDCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGK 590
            PLRDCL+QE+N+LC+LD LK VE+  +  + INGL+SSFV +LQEENP+RECTIVRTAGK
Sbjct: 321  PLRDCLIQELNMLCHLDGLKTVELNKNPSSSINGLVSSFVTLLQEENPSRECTIVRTAGK 380

Query: 589  LTPFPFNRIPEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPMS-----CYKNP 425
            LTPF FNRIPE N  +  LA+QRRQKR+NLKP ES+  E+FCPICNSP+S      +KN 
Sbjct: 381  LTPFHFNRIPEINDSNVPLATQRRQKRYNLKPTESISSESFCPICNSPLSKSDLLSFKNV 440

Query: 424  QPLDN---IFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG---NEKWIREKI 263
            +  D    +F A CCSSC++QILP++PSS+  FYS LP+ +++RAK G   N   +RE+I
Sbjct: 441  ESCDTNSVLFSAACCSSCRFQILPKDPSSVGDFYSLLPQPVVARAKCGSADNYSLLREQI 500

Query: 262  QDCLLSDGEDE 230
            +DC+LSD EDE
Sbjct: 501  KDCILSDSEDE 511


>ref|XP_017238071.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Daucus carota
            subsp. sativus]
          Length = 460

 Score =  545 bits (1404), Expect = 0.0
 Identities = 282/426 (66%), Positives = 335/426 (78%), Gaps = 16/426 (3%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEG--GG---RFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFS 1298
            CLKCKSSE  I  GG G  GG   + CADCFR NLYGKFKLAV++N MISP D VLVAFS
Sbjct: 37   CLKCKSSE--IIAGGTGFSGGTNMKLCADCFRGNLYGKFKLAVSANGMISPSDNVLVAFS 94

Query: 1297 GGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAI 1118
            GG SSRVALQFV EMQ+K+QKNFDA+  RDRSLPVFGVGVAF+DES  H + S +LDK I
Sbjct: 95   GGTSSRVALQFVHEMQVKSQKNFDAS--RDRSLPVFGVGVAFVDESAFHTLPSHELDKEI 152

Query: 1117 EDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMM 938
            E+IK IV  L+PPTK  HV+PI SI  + +N  K+ L  L+N V+D TGKEDL+  LRMM
Sbjct: 153  EEIKLIVDELSPPTKAFHVIPIESICLSNSNSAKDNLNELLNAVSDKTGKEDLMVQLRMM 212

Query: 937  SLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRD 758
            SLQK A ENGYTKLVLGSCTSR+ACHV+A+TVKGQGYSLAADIQY D+RWE+PVVLP+RD
Sbjct: 213  SLQKTALENGYTKLVLGSCTSRLACHVIASTVKGQGYSLAADIQYVDSRWEIPVVLPIRD 272

Query: 757  CLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPF 578
            C +QE+N+LC L+SLK +E+ N S+AGINGLISSFVK+LQEENP+RECTIVRTAGKLTPF
Sbjct: 273  CTLQELNMLCKLESLKTMELHNGSRAGINGLISSFVKLLQEENPSRECTIVRTAGKLTPF 332

Query: 577  PFNRIPEANVD-DTDLASQRRQKRFNLKPIESLPPEAFCPICNSPMS-------CYKNPQ 422
             FN+IPE   D +  LASQRR+K+FNLK  E LPPE++CP+C+SP+         ++N  
Sbjct: 333  HFNKIPEEADDCNLQLASQRRRKKFNLKSNELLPPESYCPLCSSPLDKNSISNLAFENGH 392

Query: 421  PLDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG---NEKWIREKIQDCL 251
                 F   CCSSCQ+QILP+EP  M+QFYS LP+ I  RAK+G   +++ IRE+IQDCL
Sbjct: 393  TSYGSFVPKCCSSCQFQILPKEPLHMEQFYSLLPQPITDRAKDGSCRSQRSIREQIQDCL 452

Query: 250  LSDGED 233
            LSD ED
Sbjct: 453  LSDNED 458


>ref|XP_006351313.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Solanum
            tuberosum]
          Length = 481

 Score =  546 bits (1406), Expect = 0.0
 Identities = 274/430 (63%), Positives = 338/430 (78%), Gaps = 20/430 (4%)
 Frame = -1

Query: 1462 CLKCK---------SSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVL 1310
            CLKCK         ++E     GG  GGRFCADCFRSNL+GKF+LAVT+NAMISP D VL
Sbjct: 52   CLKCKVNRTIAAAAAAEGGGDFGGGDGGRFCADCFRSNLFGKFRLAVTANAMISPSDNVL 111

Query: 1309 VAFSGGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDL 1130
            VAFSGG  SRVALQF  EMQ KAQKN+DA+  RDR+LPVFGVGVAFIDE  +  ++S +L
Sbjct: 112  VAFSGGTCSRVALQFTHEMQSKAQKNYDAS--RDRALPVFGVGVAFIDEKAISAVSSDNL 169

Query: 1129 DKAIEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTH 950
            D+A+E++K IV +LAPP K+ HVVP  +IYS K  D K+ L  LINTV D TGKEDLL H
Sbjct: 170  DRAVEEMKLIVSDLAPPVKQFHVVPTEAIYSLKAGDAKDRLNELINTVNDVTGKEDLLEH 229

Query: 949  LRMMSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVL 770
             RM+SLQKIA ENGYTK++LG+CTSRIACHVL ATVKG+GYSLAADIQY DARW++PVVL
Sbjct: 230  FRMLSLQKIAIENGYTKILLGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVL 289

Query: 769  PLRDCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGK 590
            PLRDC + E+N+LC+LDSLK VEVFN ++AGINGLISSFVK+LQEENP+RE TI+RTAGK
Sbjct: 290  PLRDCPIHELNMLCSLDSLKTVEVFNGTRAGINGLISSFVKLLQEENPSRESTIMRTAGK 349

Query: 589  LTPFPFNRIPEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPMS--------CY 434
            LTPF FNR+PE N  +  +AS+RRQK++NLK  ++LPPE+FCPICNSP+          +
Sbjct: 350  LTPFHFNRMPEDNDYNGQVASRRRQKKYNLKTKDALPPESFCPICNSPLKISNFGTSVTF 409

Query: 433  KNPQPLDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG---NEKWIREKI 263
             N +   N  GA CC+SCQ+Q+LP +PSS++ F S LP S++S A++G   +++ +RE+I
Sbjct: 410  DNGRTNPNGIGAACCASCQFQVLPNDPSSLEHFISLLPPSMVSEAEDGDRLSQRQLREQI 469

Query: 262  QDCLLSDGED 233
            +DCLLSD ED
Sbjct: 470  EDCLLSDNED 479


>ref|XP_023920473.1| cytoplasmic tRNA 2-thiolation protein 2 [Quercus suber]
          Length = 451

 Score =  540 bits (1391), Expect = 0.0
 Identities = 273/422 (64%), Positives = 335/422 (79%), Gaps = 11/422 (2%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            C+KCK+ E     GG+ G  FC +CFRSNLYGKF+LAVTS+AMI+P D VLVAFSGG SS
Sbjct: 37   CIKCKTKEPI---GGDEGRMFCIECFRSNLYGKFRLAVTSHAMITPTDNVLVAFSGGPSS 93

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RVALQFV E+Q K+ KNFDA+  RDRSLPVFGVGVAFIDE+ + P+ S D+D+AI+DI+ 
Sbjct: 94   RVALQFVHELQHKSLKNFDAS--RDRSLPVFGVGVAFIDETTISPLPSHDIDRAIQDIRL 151

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV NLAPP K+LHVVPI +IYS    DG   L+NL++ V+D TGKEDLL HLRM+SLQK+
Sbjct: 152  IVSNLAPPIKQLHVVPIENIYS---GDGTNRLKNLLDAVSDTTGKEDLLLHLRMLSLQKV 208

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A+ +GY K+VLG CTSRIACHV++ATVKGQGYSL ADIQY D+RWE+P+VLPLRDCL+QE
Sbjct: 209  ASVSGYNKIVLGLCTSRIACHVISATVKGQGYSLPADIQYVDSRWEIPIVLPLRDCLVQE 268

Query: 742  INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 563
            +N+LC+LD LK VE+     +GINGL+SSFV++LQEENP+RECTIVRTAGKLTPF FNRI
Sbjct: 269  LNMLCHLDGLKTVELLKGPCSGINGLVSSFVRILQEENPSRECTIVRTAGKLTPFHFNRI 328

Query: 562  PEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSP--------MSCYKNPQPLDNI 407
            PE++     LA++RRQKR+NLKP ES+  E+FCPICNSP        +S   + Q   + 
Sbjct: 329  PESDDSVIPLATRRRQKRYNLKPNESISSESFCPICNSPLNKSDMLRLSNLGSCQTSSDS 388

Query: 406  FGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG---NEKWIREKIQDCLLSDGE 236
            FGA CCSSC++QILP+ PSSMD FYS LP+ +++RAK+    N    RE+IQDCLLSD E
Sbjct: 389  FGAACCSSCRFQILPKGPSSMDHFYSLLPQPLVARAKHSSYDNLSLHREQIQDCLLSDSE 448

Query: 235  DE 230
            DE
Sbjct: 449  DE 450


>gb|POF00280.1| cytoplasmic trna 2-thiolation protein 2 [Quercus suber]
          Length = 463

 Score =  540 bits (1391), Expect = 0.0
 Identities = 273/422 (64%), Positives = 335/422 (79%), Gaps = 11/422 (2%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            C+KCK+ E     GG+ G  FC +CFRSNLYGKF+LAVTS+AMI+P D VLVAFSGG SS
Sbjct: 49   CIKCKTKEPI---GGDEGRMFCIECFRSNLYGKFRLAVTSHAMITPTDNVLVAFSGGPSS 105

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RVALQFV E+Q K+ KNFDA+  RDRSLPVFGVGVAFIDE+ + P+ S D+D+AI+DI+ 
Sbjct: 106  RVALQFVHELQHKSLKNFDAS--RDRSLPVFGVGVAFIDETTISPLPSHDIDRAIQDIRL 163

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV NLAPP K+LHVVPI +IYS    DG   L+NL++ V+D TGKEDLL HLRM+SLQK+
Sbjct: 164  IVSNLAPPIKQLHVVPIENIYS---GDGTNRLKNLLDAVSDTTGKEDLLLHLRMLSLQKV 220

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A+ +GY K+VLG CTSRIACHV++ATVKGQGYSL ADIQY D+RWE+P+VLPLRDCL+QE
Sbjct: 221  ASVSGYNKIVLGLCTSRIACHVISATVKGQGYSLPADIQYVDSRWEIPIVLPLRDCLVQE 280

Query: 742  INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 563
            +N+LC+LD LK VE+     +GINGL+SSFV++LQEENP+RECTIVRTAGKLTPF FNRI
Sbjct: 281  LNMLCHLDGLKTVELLKGPCSGINGLVSSFVRILQEENPSRECTIVRTAGKLTPFHFNRI 340

Query: 562  PEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSP--------MSCYKNPQPLDNI 407
            PE++     LA++RRQKR+NLKP ES+  E+FCPICNSP        +S   + Q   + 
Sbjct: 341  PESDDSVIPLATRRRQKRYNLKPNESISSESFCPICNSPLNKSDMLRLSNLGSCQTSSDS 400

Query: 406  FGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG---NEKWIREKIQDCLLSDGE 236
            FGA CCSSC++QILP+ PSSMD FYS LP+ +++RAK+    N    RE+IQDCLLSD E
Sbjct: 401  FGAACCSSCRFQILPKGPSSMDHFYSLLPQPLVARAKHSSYDNLSLHREQIQDCLLSDSE 460

Query: 235  DE 230
            DE
Sbjct: 461  DE 462


>ref|XP_004249262.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Solanum
            lycopersicum]
          Length = 478

 Score =  538 bits (1386), Expect = 0.0
 Identities = 269/427 (62%), Positives = 334/427 (78%), Gaps = 17/427 (3%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEG------GGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAF 1301
            CLKCK +       G G       GRFCADCFRSNL+GKF+LAVT+NAMISP D VLVAF
Sbjct: 52   CLKCKVNRTIAAADGGGDFGGGDAGRFCADCFRSNLFGKFRLAVTANAMISPSDNVLVAF 111

Query: 1300 SGGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKA 1121
            SGG  SRVALQF  EMQ KAQKN+DA+  RDR+LPVFGVGVAFIDE  +  ++S +LD+A
Sbjct: 112  SGGTCSRVALQFTHEMQSKAQKNYDAS--RDRALPVFGVGVAFIDEKAISAVSSDNLDRA 169

Query: 1120 IEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRM 941
            +E+++ IV +LAPP K+ HVVP  +IYS    D K+ L  LINTV D TGKEDLL H RM
Sbjct: 170  VEEMELIVSDLAPPVKQFHVVPTEAIYSLDAGDSKDRLNELINTVNDVTGKEDLLEHFRM 229

Query: 940  MSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLR 761
            +SLQKIA ENGYTK++LG+CTSRIACHVL ATVKG+GYSLAADIQY DARW++PVVLPLR
Sbjct: 230  LSLQKIAIENGYTKILLGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVLPLR 289

Query: 760  DCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTP 581
            DC + E+N+LC+LDSLK VEVFN ++AGINGLISSFVK+LQEENP+RE TI+RTAGKLTP
Sbjct: 290  DCPIHELNMLCSLDSLKTVEVFNGTRAGINGLISSFVKLLQEENPSRESTIMRTAGKLTP 349

Query: 580  FPFNRIPEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPMSC--------YKNP 425
            F FNR+PE N  +  +AS+RRQK++NLK  ++LPPE+FCPICNSP+          ++N 
Sbjct: 350  FHFNRMPEDNDYNGQVASRRRQKKYNLKTKDALPPESFCPICNSPLKISSFGTSINFENG 409

Query: 424  QPLDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG---NEKWIREKIQDC 254
            +   N  GA CC+SCQ+Q+LP +PSS++ F S LP S++S+A +    +++ +RE+I+DC
Sbjct: 410  KTNPNGIGAACCASCQFQVLPNDPSSLEHFISLLPPSMVSQAGDNDRLSQRQLREQIEDC 469

Query: 253  LLSDGED 233
            LLSD ED
Sbjct: 470  LLSDNED 476


>ref|XP_015089096.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Solanum
            pennellii]
          Length = 478

 Score =  537 bits (1383), Expect = 0.0
 Identities = 269/427 (62%), Positives = 333/427 (77%), Gaps = 17/427 (3%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEG------GGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAF 1301
            CLKCK +       G G       GRFCADCFRSNL+GKF+LAVT+NAMISP D VLVAF
Sbjct: 52   CLKCKVNRTIAAADGGGDFGGGDAGRFCADCFRSNLFGKFRLAVTANAMISPSDNVLVAF 111

Query: 1300 SGGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKA 1121
            SGG  SRVALQF  EMQ KAQKN+DA+  RDR+LPVFGVGVAFIDE  +  ++S +LD+A
Sbjct: 112  SGGTCSRVALQFTHEMQSKAQKNYDAS--RDRALPVFGVGVAFIDEKAISAVSSDNLDRA 169

Query: 1120 IEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRM 941
            +E++K IV +LAPP K+ HVVP  +IYS    D K+ L  LINTV D TGKEDLL H RM
Sbjct: 170  VEEMKLIVSDLAPPVKQFHVVPTEAIYSLDAGDSKDRLNELINTVNDVTGKEDLLEHFRM 229

Query: 940  MSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLR 761
            +SLQKIA ENGYTK++LG+CTSRIACHVL ATVKG+GYSLAADIQY DARW++PVVLPLR
Sbjct: 230  LSLQKIAIENGYTKIILGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVLPLR 289

Query: 760  DCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTP 581
            DC + E+N+LC+LDSLK VEVFN ++AGINGLISS VK+LQEENP+RE TI+RTAGKLTP
Sbjct: 290  DCPIHELNMLCSLDSLKTVEVFNGTRAGINGLISSLVKLLQEENPSRESTIMRTAGKLTP 349

Query: 580  FPFNRIPEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPMSC--------YKNP 425
            F FNR+PE N  +  +AS+RRQK++NLK  ++LPPE+FCPICNSP+          ++N 
Sbjct: 350  FHFNRMPEDNDYNGQVASRRRQKKYNLKTKDALPPESFCPICNSPLKISSFGTSINFENG 409

Query: 424  QPLDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG---NEKWIREKIQDC 254
            +   N  GA CCSSCQ+Q+LP +PSS++ F S LP +++S+A +    +++ +RE+I+DC
Sbjct: 410  KTNPNGIGAACCSSCQFQVLPNDPSSLEHFISLLPPAMVSQAGDNDRLSQRQLREQIEDC 469

Query: 253  LLSDGED 233
            LLSD ED
Sbjct: 470  LLSDYED 476


>ref|XP_006421388.1| cytoplasmic tRNA 2-thiolation protein 2 isoform X1 [Citrus
            clementina]
 gb|ESR34628.1| hypothetical protein CICLE_v10004945mg [Citrus clementina]
          Length = 455

 Score =  536 bits (1380), Expect = 0.0
 Identities = 268/422 (63%), Positives = 335/422 (79%), Gaps = 11/422 (2%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            C+KCK++E T   G +G  + C DCFRSNL+GKF+LAV SNA+I+P D VLVAFSGG SS
Sbjct: 38   CVKCKANEPTPGAGEDG--KHCLDCFRSNLFGKFRLAVASNALITPADNVLVAFSGGPSS 95

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RVALQFV E+Q +AQKNFDA+  +DRSLPVFGVGV F+DE+  +P+ S ++D AI++IK 
Sbjct: 96   RVALQFVHELQQRAQKNFDAS--KDRSLPVFGVGVVFVDETAYYPVPSSEIDNAIQEIKL 153

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV NL+PPTKELHV+PI SI+ +   DG+E  + L+++V+DATGKEDLL  LRM+SLQK 
Sbjct: 154  IVSNLSPPTKELHVIPIESIFCSNPCDGRERFKKLVDSVSDATGKEDLLLQLRMLSLQKF 213

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A+ENGY +L+LG CTSRIACHV+ ATVKG+GYSL ADIQYADARWE+PVVLPLRDCL QE
Sbjct: 214  ASENGYNRLLLGLCTSRIACHVITATVKGRGYSLPADIQYADARWEIPVVLPLRDCLAQE 273

Query: 742  INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 563
            +N+LC LD LK VE+ N + +GINGL+SSFVK+LQEENP+RE TI+RTAGKLTPF FN+I
Sbjct: 274  LNMLCQLDCLKTVELLNQTHSGINGLVSSFVKILQEENPSRESTIMRTAGKLTPFHFNKI 333

Query: 562  PEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSP--------MSCYKNPQPLDNI 407
            PE N     LASQRRQKRFNLKP ES+  E+FC +C SP        +S + N +  D I
Sbjct: 334  PELNDSSVPLASQRRQKRFNLKPNESISSESFCSLCYSPLNQSDLTSLSSHDNCKNSD-I 392

Query: 406  FGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAK---NGNEKWIREKIQDCLLSDGE 236
            F A CCSSC++QI P++PSSM++FYS LPE +++RAK   NG+   +RE+IQD LLSD E
Sbjct: 393  FVAACCSSCRFQIFPKDPSSMEKFYSLLPEPLVARAKHVRNGDSSLLREQIQDFLLSDSE 452

Query: 235  DE 230
            D+
Sbjct: 453  DD 454


>ref|XP_019224820.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Nicotiana
            attenuata]
 gb|OIT33086.1| cytoplasmic trna 2-thiolation protein 2 [Nicotiana attenuata]
          Length = 478

 Score =  536 bits (1380), Expect = 0.0
 Identities = 271/430 (63%), Positives = 335/430 (77%), Gaps = 20/430 (4%)
 Frame = -1

Query: 1462 CLKCK---------SSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVL 1310
            C+KCK         S+E      GE  GRFCADCFRSNLYGKF+ AVTSNAMISP+D VL
Sbjct: 51   CVKCKVNKTIAAAASAEDGGDFSGEDSGRFCADCFRSNLYGKFRFAVTSNAMISPLDNVL 110

Query: 1309 VAFSGGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDL 1130
            VAFSGG  SRVALQF  EMQ KAQKN+DA+   DR+LPVFGVGVAFIDE  +  ++S DL
Sbjct: 111  VAFSGGTCSRVALQFTHEMQSKAQKNYDASP--DRALPVFGVGVAFIDEKAISAVSSDDL 168

Query: 1129 DKAIEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTH 950
            D+AIE +K I+ +LAPP K+ HVVP  +IYS K  D K+ L  LIN V+D TGKEDLL H
Sbjct: 169  DRAIEKMKLILSDLAPPVKQFHVVPAEAIYSLKAGDAKDRLNELINAVSDVTGKEDLLEH 228

Query: 949  LRMMSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVL 770
            LRM+SLQKIA E+GYTK++LG+CTSRIACHVL ATVKG+GYSLAADIQY DARW++PVVL
Sbjct: 229  LRMLSLQKIAVEHGYTKVLLGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVL 288

Query: 769  PLRDCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGK 590
            PLRDCL+ E+N LC+LDSL IVEVFN +Q+GINGL+SSFVK+LQEENP+RE TI+RTAGK
Sbjct: 289  PLRDCLIHELNTLCSLDSLMIVEVFNGTQSGINGLVSSFVKLLQEENPSRESTIMRTAGK 348

Query: 589  LTPFPFNRIPEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPMS--------CY 434
            LTPF FNR+ E N  +  +ASQRRQK+++LK  ++LPPE+FC IC+SP+          +
Sbjct: 349  LTPFHFNRMSEDNNYNDQVASQRRQKKYSLKTNDALPPESFCRICSSPLKISTFVTSISF 408

Query: 433  KNPQPLDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNG---NEKWIREKI 263
             N +   N  GA CC+SCQ+Q+LPE+PSS++ F S LP S++S A++G   +++ +RE+I
Sbjct: 409  DNAKTSLNARGAACCASCQFQVLPEDPSSLEHFISLLPPSMVSHAEDGDHLSQRQLREQI 468

Query: 262  QDCLLSDGED 233
            +DCLLSD ED
Sbjct: 469  EDCLLSDNED 478


>ref|XP_007028730.2| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Theobroma cacao]
          Length = 463

 Score =  535 bits (1377), Expect = 0.0
 Identities = 271/423 (64%), Positives = 335/423 (79%), Gaps = 11/423 (2%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            C+KCKS+E      G    RFC DCF++NLYGKFK AV+ NAMI+P DKVLVAFSGG SS
Sbjct: 43   CVKCKSNEPVCCGIGGDDARFCKDCFKTNLYGKFKQAVSYNAMITPSDKVLVAFSGGPSS 102

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RV LQFV E+Q +AQ NFDA+  +DRSLPVFGVGVAFIDES +H  AS D +KAIEDI+ 
Sbjct: 103  RVVLQFVHEIQCRAQTNFDAS--KDRSLPVFGVGVAFIDESSIHSFASQDAEKAIEDIRS 160

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV NLAPP KEL+VVPI +I+S+   DGKE ++ L++ V+D TGKEDLL HLRM+SLQ++
Sbjct: 161  IVSNLAPPEKELYVVPIENIFSSDNIDGKERMKKLLDAVSDVTGKEDLLIHLRMLSLQRV 220

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A++N YT++VLGSCTSRIACHV++ATVKGQGYSL+ADIQY D+RW+VPVVLPLRDC  QE
Sbjct: 221  ASQNRYTRVVLGSCTSRIACHVISATVKGQGYSLSADIQYVDSRWQVPVVLPLRDCPAQE 280

Query: 742  INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 563
            +N LC+LD LKIVE+ N   + INGL+SSFVKVLQEENP+RECTIVRTAGKLTPF FNRI
Sbjct: 281  LNTLCSLDGLKIVELLNGPCSSINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHFNRI 340

Query: 562  PEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPM------SCYKNPQPLDN--I 407
            P+ +     LA++RRQKR ++KP  SL  E+FCPICNSP+      S  ++ Q  +N  I
Sbjct: 341  PDIHDSSVPLATRRRQKRHSIKPNGSLSSESFCPICNSPLNKSNFSSSLRSLQSNENSDI 400

Query: 406  FGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNGNE---KWIREKIQDCLLSDGE 236
            F A  CSSCQ+QI+P+EPS M+QF S LP+ +I+RAK+G+      +RE+IQ+ LLSDGE
Sbjct: 401  FAAAACSSCQFQIIPKEPSLMEQFVSLLPQPLITRAKHGDRGDFSLLREQIQEFLLSDGE 460

Query: 235  DEV 227
            DE+
Sbjct: 461  DEI 463


>gb|EOY09232.1| Adenine nucleotide alpha hydrolases-like superfamily protein,
            putative [Theobroma cacao]
          Length = 464

 Score =  534 bits (1375), Expect = 0.0
 Identities = 273/424 (64%), Positives = 336/424 (79%), Gaps = 12/424 (2%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFSGGASS 1283
            C+KCKS+E      G    RFC DCF++NLYGKFK AV+ NAMI+P DKVLVAFSGG SS
Sbjct: 43   CVKCKSNEPVCCGIGGDDARFCKDCFKTNLYGKFKQAVSYNAMITPSDKVLVAFSGGPSS 102

Query: 1282 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 1103
            RV LQFV EMQ +AQKNFDA+  +DRSLPVFGVGVAFIDES +H  AS D +KAIEDI+ 
Sbjct: 103  RVVLQFVHEMQCRAQKNFDAS--KDRSLPVFGVGVAFIDESSIHSFASQDAEKAIEDIRS 160

Query: 1102 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 923
            IV NLAPP KEL+VVPI +I+S+   DGKE ++ L++ V+D TGKEDLL HLRM+SLQ++
Sbjct: 161  IVSNLAPPEKELYVVPIENIFSSDNIDGKERMKKLLDAVSDVTGKEDLLIHLRMLSLQRV 220

Query: 922  ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 743
            A++N YT++VLGSCTSRIACHV++ATVKGQGYSL+ADIQY D+RW+VPVVLPLRDC  QE
Sbjct: 221  ASQNRYTRVVLGSCTSRIACHVISATVKGQGYSLSADIQYVDSRWQVPVVLPLRDCPAQE 280

Query: 742  INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 563
            +N LC+LD LKIVE+ N   + INGL+SSFVKVLQEENP+RECTIVRTAGKLTPF FNRI
Sbjct: 281  LNTLCSLDGLKIVELLNGPCSSINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHFNRI 340

Query: 562  PEANVDDTDLASQRRQKRFNLKPIESLPPEAFCPICNSPM------SCYKNPQPLDN--I 407
            P+ +     LA++RRQKR ++KP  SL  E+FCPICNSP+      S  ++ Q  +N  I
Sbjct: 341  PDIHDSSVPLATRRRQKRHSIKPNGSLSSESFCPICNSPLNKSNFSSSLRSLQSNENSDI 400

Query: 406  F-GATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKNGNE---KWIREKIQDCLLSDG 239
            F  A  CSSCQ+QI+P+EPS M+QF S LP+ +I+RAK+G+      +RE+IQ+ LLSDG
Sbjct: 401  FAAAAACSSCQFQIIPKEPSLMEQFVSLLPQPLITRAKHGDRGDFSLLREQIQEFLLSDG 460

Query: 238  EDEV 227
            EDE+
Sbjct: 461  EDEI 464


>gb|KZN00307.1| hypothetical protein DCAR_009061 [Daucus carota subsp. sativus]
          Length = 477

 Score =  534 bits (1376), Expect = 0.0
 Identities = 282/443 (63%), Positives = 335/443 (75%), Gaps = 33/443 (7%)
 Frame = -1

Query: 1462 CLKCKSSEATIFTGGEG--GG---RFCADCFRSNLYGKFKLAVTSNAMISPVDKVLVAFS 1298
            CLKCKSSE  I  GG G  GG   + CADCFR NLYGKFKLAV++N MISP D VLVAFS
Sbjct: 37   CLKCKSSE--IIAGGTGFSGGTNMKLCADCFRGNLYGKFKLAVSANGMISPSDNVLVAFS 94

Query: 1297 GGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAI 1118
            GG SSRVALQFV EMQ+K+QKNFDA+  RDRSLPVFGVGVAF+DES  H + S +LDK I
Sbjct: 95   GGTSSRVALQFVHEMQVKSQKNFDAS--RDRSLPVFGVGVAFVDESAFHTLPSHELDKEI 152

Query: 1117 EDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMM 938
            E+IK IV  L+PPTK  HV+PI SI  + +N  K+ L  L+N V+D TGKEDL+  LRMM
Sbjct: 153  EEIKLIVDELSPPTKAFHVIPIESICLSNSNSAKDNLNELLNAVSDKTGKEDLMVQLRMM 212

Query: 937  SLQKIATENGYTKLVLGSCTSRIACHVLAATV-----------------KGQGYSLAADI 809
            SLQK A ENGYTKLVLGSCTSR+ACHV+A+TV                 KGQGYSLAADI
Sbjct: 213  SLQKTALENGYTKLVLGSCTSRLACHVIASTVKNKALSSKLIVSGFMYAKGQGYSLAADI 272

Query: 808  QYADARWEVPVVLPLRDCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEEN 629
            QY D+RWE+PVVLP+RDC +QE+N+LC L+SLK +E+ N S+AGINGLISSFVK+LQEEN
Sbjct: 273  QYVDSRWEIPVVLPIRDCTLQELNMLCKLESLKTMELHNGSRAGINGLISSFVKLLQEEN 332

Query: 628  PARECTIVRTAGKLTPFPFNRIPEANVD-DTDLASQRRQKRFNLKPIESLPPEAFCPICN 452
            P+RECTIVRTAGKLTPF FN+IPE   D +  LASQRR+K+FNLK  E LPPE++CP+C+
Sbjct: 333  PSRECTIVRTAGKLTPFHFNKIPEEADDCNLQLASQRRRKKFNLKSNELLPPESYCPLCS 392

Query: 451  SPMS-------CYKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESIISRAKN 293
            SP+         ++N       F   CCSSCQ+QILP+EP  M+QFYS LP+ I  RAK+
Sbjct: 393  SPLDKNSISNLAFENGHTSYGSFVPKCCSSCQFQILPKEPLHMEQFYSLLPQPITDRAKD 452

Query: 292  G---NEKWIREKIQDCLLSDGED 233
            G   +++ IRE+IQDCLLSD ED
Sbjct: 453  GSCRSQRSIREQIQDCLLSDNED 475