BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00030971
         (1606 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KVH95431.1| Zinc finger, MIZ-type [Cynara cardunculus var. sc...   556   0.0  
ref|XP_022025943.1| E4 SUMO-protein ligase PIAL2-like [Helianthu...   540   0.0  
ref|XP_021969194.1| E4 SUMO-protein ligase PIAL2-like isoform X5...   538   0.0  
ref|XP_023738525.1| E4 SUMO-protein ligase PIAL2, partial [Lactu...   526   e-176
ref|XP_021969193.1| E4 SUMO-protein ligase PIAL2-like isoform X4...   526   e-176
ref|XP_021969192.1| E4 SUMO-protein ligase PIAL2-like isoform X3...   525   e-175
ref|XP_021969191.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   523   e-175
gb|PLY70155.1| hypothetical protein LSAT_3X11501 [Lactuca sativa]     518   e-173
ref|XP_021969190.1| E4 SUMO-protein ligase PIAL2-like isoform X1...   494   e-163
ref|XP_022002147.1| E4 SUMO-protein ligase PIAL2-like isoform X3...   355   e-109
ref|XP_022002150.1| E4 SUMO-protein ligase PIAL2-like isoform X6...   342   e-105
ref|XP_022002149.1| E4 SUMO-protein ligase PIAL2-like isoform X5...   342   e-105
ref|XP_022002148.1| E4 SUMO-protein ligase PIAL2-like isoform X4...   342   e-105
ref|XP_022002145.1| E4 SUMO-protein ligase PIAL2-like isoform X1...   342   e-104
ref|XP_022002146.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   341   e-104
ref|XP_023752744.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   334   e-103
ref|XP_023752743.1| E4 SUMO-protein ligase PIAL2-like isoform X1...   334   e-102
gb|PLY93837.1| hypothetical protein LSAT_6X84940 [Lactuca sativa]     334   e-102
ref|XP_019151668.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   324   2e-97
ref|XP_019151669.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   322   1e-96

>gb|KVH95431.1| Zinc finger, MIZ-type [Cynara cardunculus var. scolymus]
          Length = 830

 Score =  556 bits (1432), Expect = 0.0
 Identities = 333/601 (55%), Positives = 379/601 (63%), Gaps = 66/601 (10%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GV++RTCV KD GPQ+PT VT+MLKYGSNLLQVVGQF+ RYI+ IAFMSV+SNP+ PTIP
Sbjct: 233  GVDRRTCVYKDPGPQIPTLVTHMLKYGSNLLQVVGQFSERYIIVIAFMSVVSNPSCPTIP 292

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            DYIPP AA PD DNEI+EGPSR+SLKCPI  SRIKIP          CFDFNNYVG+N+R
Sbjct: 293  DYIPPAAALPDSDNEIVEGPSRISLKCPI--SRIKIP----------CFDFNNYVGMNSR 340

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPENPEKPK 540
            RPLWRCPHCSQSV F DIRIDQSM  VLKEVGED+SYVKI +DGSWE+VTES E+  KP 
Sbjct: 341  RPLWRCPHCSQSVCFTDIRIDQSM--VLKEVGEDVSYVKISSDGSWEAVTESNEHTIKPN 398

Query: 541  DAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPA---GESSAVNK-------- 687
            DA   +QET IQ VDDIMDLTE               PSPA   GESS +N         
Sbjct: 399  DAQPLHQETTIQSVDDIMDLTEGDNEADTCDRDEDKKPSPAEFHGESSILNSTPVSMHIN 458

Query: 688  ----NAAPHMEDGFWTEYYSMGLPGTRTNNSRSDVSEV----------------VQRNQQ 807
                N APHMEDGFW E+YS  L GTRT N+RSDV+EV                +QRN  
Sbjct: 459  NVHGNIAPHMEDGFWREFYSSTL-GTRTTNTRSDVNEVRSVRDAFASLDREIEDLQRNNL 517

Query: 808  MFNSITLGQTQSQIPVSXXXXXXXXXXXXXXXXLYTRY---QNWVTRTPNAVQALPAQSS 978
            +FNS+T GQT +    S                 Y RY    N+VTRT  A+QALPAQSS
Sbjct: 518  IFNSLTPGQTAA----SNSMGLQQYENSNNTGNGYVRYPTPANYVTRTAIAIQALPAQSS 573

Query: 979  ATVANGDRQQH---LSRPQLNQHQVSRM--------MSSQNQGQRDH------PVPVL-- 1101
            A +  GDRQQH     R  LNQHQVS M        M SQNQG RDH        P L  
Sbjct: 574  ARILEGDRQQHQQQFVRSHLNQHQVSLMTSAPSPQHMGSQNQGHRDHSHLLSQTSPQLGG 633

Query: 1102 --PAQQHISNGRH---IPSAHFNSYQQHSINQRPTSYPVRSPAHLSPQYRPVG------P 1248
               + Q +S GRH   +P+  FNS+QQ S+NQR T  PVR  +  SPQ R +G      P
Sbjct: 634  RSASPQRLSGGRHTSPLPTDRFNSHQQQSMNQR-TPLPVRPSSQPSPQVRLIGAHGGGNP 692

Query: 1249 MTS--SQNPQYSMADVHRATSQATTHTSSIPTSIVTPGPSVTVASTGEQRGGSVDGPGEE 1422
            MTS  S  PQYS+A + RAT+Q  + T   P+ I T G SV V S GEQRG SVD  GE+
Sbjct: 693  MTSPASNQPQYSLASIQRATNQFLSRTPPTPSPIQTSGSSVPVTSMGEQRGNSVDASGEQ 752

Query: 1423 NWRPTGRMRGSLSGRPYSETLNQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMANNI 1602
            NWRP+GRMRGSLSGR Y+E LNQ II                TPRPFIPPHLQ LMANN+
Sbjct: 753  NWRPSGRMRGSLSGRAYTEALNQFII-HPNQPVQAARPPVLNTPRPFIPPHLQVLMANNL 811

Query: 1603 R 1605
            +
Sbjct: 812  K 812


>ref|XP_022025943.1| E4 SUMO-protein ligase PIAL2-like [Helianthus annuus]
 gb|OTF84876.1| putative RING/U-box superfamily protein [Helianthus annuus]
          Length = 770

 Score =  540 bits (1392), Expect = 0.0
 Identities = 302/551 (54%), Positives = 351/551 (63%), Gaps = 18/551 (3%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GV+KRTCV KD GPQMPT VT+MLKYGSNLLQVVGQF  RYI+AIAFM  +SNP++P IP
Sbjct: 232  GVDKRTCVYKDFGPQMPTHVTHMLKYGSNLLQVVGQFNARYIIAIAFMRTVSNPSYPIIP 291

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            DY+ PVAA PD DNEIIEGPSR+SLKCPISFSRIK PVKGHSCKHLQCFDFNNYV +N++
Sbjct: 292  DYVQPVAAQPDSDNEIIEGPSRISLKCPISFSRIKNPVKGHSCKHLQCFDFNNYVELNSK 351

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPEN--PEK 534
            RPLWRCPHCSQSV FHD+RIDQSMVKVLKEVGED+SYVKI  DGSWE+VT++  N   EK
Sbjct: 352  RPLWRCPHCSQSVCFHDLRIDQSMVKVLKEVGEDVSYVKISGDGSWEAVTDNIGNELTEK 411

Query: 535  PKDAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAGESSAVNKNAAPHMEDG 714
            P D    NQET  ++VDD+MDLTEN               SPAG SS  NKN APHMED 
Sbjct: 412  PNDTQPLNQETTSRNVDDVMDLTENDNEIDKFEDNDDKKSSPAGVSSVTNKNTAPHMEDE 471

Query: 715  FWTEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQ----IPV-----SXXX 867
            FW E+YS          +R D  +V+QRN+ +FNS+T GQ+QSQ     PV     S   
Sbjct: 472  FWGEFYS---------PARLDAGDVIQRNRLLFNSLTRGQSQSQSQTETPVSGNTPSQQN 522

Query: 868  XXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQLNQHQVS 1047
                          Y    +WVT T NA+QALPAQ+SA   +GD + + SR  LN    S
Sbjct: 523  ENSNNNNNNDGNVRYPTSTSWVTNTRNAIQALPAQNSARTVDGDIRHNFSRSHLNHRSAS 582

Query: 1048 RMMSSQNQGQRDHPVPVLPAQQHISNGRHIPSAHFNSYQQHSINQRPTSYPVRSPAHLSP 1227
             + + +                            F+S+Q  S+NQR    PVRS    +P
Sbjct: 583  PLSTDR----------------------------FSSHQTQSLNQR-AHLPVRSSGQPAP 613

Query: 1228 QYR--PVGPMT---SSQNPQYSMADVHRATSQATTHTSSIPTSIVTPG-PSVTVASTGEQ 1389
              R   VGPMT   SS + QYS++ + RATSQ  + TSS+   I T G  SVTVA+TG  
Sbjct: 614  HVRLGGVGPMTSPASSPSAQYSLSAIQRATSQIRSPTSSVTPPIQTSGSSSVTVAATGNH 673

Query: 1390 RGGSVDGPGEENWRPTGRMRGSLSGRPYSETLNQN-IIXXXXXXXXXXXXXXXXTPRPFI 1566
            R  SVDG  EENWRPTGRMRGSLSG+ Y+E L    II                TPRPFI
Sbjct: 674  RETSVDGSAEENWRPTGRMRGSLSGQQYTEALKHKVIIQPSEPVQAARPPPVLNTPRPFI 733

Query: 1567 PPHLQALMANN 1599
            PPHLQALMANN
Sbjct: 734  PPHLQALMANN 744


>ref|XP_021969194.1| E4 SUMO-protein ligase PIAL2-like isoform X5 [Helianthus annuus]
          Length = 795

 Score =  538 bits (1386), Expect = 0.0
 Identities = 312/564 (55%), Positives = 359/564 (63%), Gaps = 29/564 (5%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GV++RTC  KD  PQMPTPVT+MLKYGSNLLQVVGQF GRYI+ I FMSV+SNP+FPTIP
Sbjct: 236  GVDRRTCTYKDPAPQMPTPVTHMLKYGSNLLQVVGQFNGRYIIVIGFMSVVSNPSFPTIP 295

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            DY+PP+AA  DPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSC HLQCFDFNNY+GIN+R
Sbjct: 296  DYVPPIAAQSDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCNHLQCFDFNNYMGINSR 355

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTES----PENP 528
            RPLWRCPHCSQSV F DIRIDQSMVKVLKEVGED+SYVKI  DGSW++VTE+     E+ 
Sbjct: 356  RPLWRCPHCSQSVCFLDIRIDQSMVKVLKEVGEDVSYVKISGDGSWQAVTENISNGNEHI 415

Query: 529  EKPKDAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAGESSAVNKNAAPHME 708
            EKP DAPSSNQET  Q+VDDIMDLTEN              PSPAGESS  N N APH +
Sbjct: 416  EKPNDAPSSNQETATQNVDDIMDLTENDDEMEKVDKDYDQMPSPAGESSLTNNNIAPHTD 475

Query: 709  DGFWTEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSI--TLGQTQSQIPVSXXXXXXXX 882
            DG+W  Y S  L  T T N+RSDV + +QR   +FNS+  + GQ QSQ+ VS        
Sbjct: 476  DGYWRRYCSSALV-TGTTNTRSDVFDAIQRQHLIFNSLGQSQGQGQSQMHVSNNTQSQHN 534

Query: 883  XXXXXXXXLYTRY---QNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQLNQHQVSRM 1053
                     + RY      +TR PNAVQALPAQ+SA           S+P          
Sbjct: 535  ENSNNNGNGHVRYPTSATLITRIPNAVQALPAQNSAITGQ-------SQPD------HSF 581

Query: 1054 MSSQNQGQRDHPVPV---LPAQQHISNGRH---IPSAHFNSYQQHSINQRPTSYPVRSPA 1215
            +SSQ+Q Q   P+ V     + Q IS  R+   + +  FNS Q  S+NQR    PVRS  
Sbjct: 582  LSSQSQSQSQTPLQVGARSASPQPISGSRYTSPLSTDRFNSNQYQSLNQR-AHLPVRSSG 640

Query: 1216 HLSPQYRPVG---------PMTSSQNPQYSMADVHRATSQATTHTSSIPTSIVTPGPSVT 1368
              SP  R  G          + SSQ+P Y    + R+TSQ  + +SS P           
Sbjct: 641  QPSPHVRLGGAHVGVSTMTSLASSQSPTY----IQRSTSQIMSQSSSGP----------- 685

Query: 1369 VASTGEQRGGSVDGP----GEENWRPTGRMRGSLSGRPYSETL-NQNIIXXXXXXXXXXX 1533
            VA+T +QRG +VD P     EENWRP GRMRGSLSG  Y+E L NQ II           
Sbjct: 686  VAATTDQRGSTVDAPPAAAAEENWRPAGRMRGSLSGWQYTEALRNQFIIQPSEPVQAARP 745

Query: 1534 XXXXXTPRPFIPPHLQALMANNIR 1605
                 TPRPFIPPHLQALMANN R
Sbjct: 746  PPVLNTPRPFIPPHLQALMANNPR 769


>ref|XP_023738525.1| E4 SUMO-protein ligase PIAL2, partial [Lactuca sativa]
          Length = 787

 Score =  526 bits (1355), Expect = e-176
 Identities = 302/544 (55%), Positives = 350/544 (64%), Gaps = 9/544 (1%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GVE+RTC+ +D GPQ PTPVT+MLKYGSNLLQVVGQF GRYI+ IAFMSV+SNPT P I 
Sbjct: 262  GVERRTCIFRDPGPQAPTPVTHMLKYGSNLLQVVGQFNGRYIIVIAFMSVVSNPTCPPIL 321

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            DY+P  AA PD DNEIIEGPSR+SLKCPISFSRIKIPVKGH+CKHLQCFDFNNYVGIN R
Sbjct: 322  DYVPLAAAAPDSDNEIIEGPSRISLKCPISFSRIKIPVKGHTCKHLQCFDFNNYVGINTR 381

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPENPEKPK 540
            RP WRCPHCSQSV FHDIRIDQ MVKVLKEVGED+S+VKI +DGSWE+VTES EN +K  
Sbjct: 382  RPQWRCPHCSQSVCFHDIRIDQRMVKVLKEVGEDVSHVKISSDGSWEAVTESNENSDKQN 441

Query: 541  DAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAGESSAVNKNAAPHMEDGFW 720
            DAP  +Q+T IQ++DDIMDLTE               PS     + VNKN  PHMEDGFW
Sbjct: 442  DAPPVHQDTSIQNIDDIMDLTEG-----DNNEVDDKKPS----VNNVNKNIPPHMEDGFW 492

Query: 721  TEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQIPVSXXXXXXXXXXXXXX 900
             E+YS  LP TR  N+R D ++++   + +FNSI  GQ+QSQ                  
Sbjct: 493  REFYSPSLP-TRIANTRPDAADLIP-GRPIFNSIP-GQSQSQ-------SQSQNDNSNNG 542

Query: 901  XXLYTRYQNW---VTRTPNAVQALPAQSSATVANGDRQQHLSRPQLNQHQVSRMMSSQNQ 1071
              +++RY      VTR PN +QALPAQ+S+  A+ DRQQ      L   Q +RM S    
Sbjct: 543  SNVHSRYPTHMLNVTRAPNTIQALPAQTSSRSADVDRQQQNFSQILTNLQSARMSSQ--- 599

Query: 1072 GQRDHPVPVLPAQQHISNGRHIPSAHFNSYQQHSINQR-PTSYPVRSPAHLSPQYRP--- 1239
                  +P    QQ   N   +P   F S+   SINQR P   P+R  A   P  RP   
Sbjct: 600  ------IP----QQKGRNMSPLPPDRFWSHPPQSINQRTPVPVPMRPSAPTPPAARPPGG 649

Query: 1240 --VGPMTSSQNPQYSMADVHRATSQATTHTSSIPTSIVTPGPSVTVASTGEQRGGSVDGP 1413
              VGP   +    YS+AD+ RAT+     T S P SI TP PS+ V ST +QR       
Sbjct: 650  GGVGPNPMTSQSHYSLADIQRATN--VLRTPSTPVSIQTPAPSMPVTSTADQR--DPGSS 705

Query: 1414 GEENWRPTGRMRGSLSGRPYSETLNQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMA 1593
            G+ NWRPTGRMRGSLSGR YSE LNQ II                TPRPFIPPHLQALMA
Sbjct: 706  GDANWRPTGRMRGSLSGRAYSEALNQYII-QPNQPVQAARPPVLNTPRPFIPPHLQALMA 764

Query: 1594 NNIR 1605
            NN+R
Sbjct: 765  NNLR 768


>ref|XP_021969193.1| E4 SUMO-protein ligase PIAL2-like isoform X4 [Helianthus annuus]
          Length = 829

 Score =  526 bits (1355), Expect = e-176
 Identities = 311/597 (52%), Positives = 358/597 (59%), Gaps = 62/597 (10%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GV++RTC  KD  PQMPTPVT+MLKYGSNLLQVVGQF GRYI+ I FMSV+SNP+FPTIP
Sbjct: 236  GVDRRTCTYKDPAPQMPTPVTHMLKYGSNLLQVVGQFNGRYIIVIGFMSVVSNPSFPTIP 295

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            DY+PP+AA  DPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSC HLQCFDFNNY+GIN+R
Sbjct: 296  DYVPPIAAQSDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCNHLQCFDFNNYMGINSR 355

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTES----PENP 528
            RPLWRCPHCSQSV F DIRIDQSMVKVLKEVGED+SYVKI  DGSW++VTE+     E+ 
Sbjct: 356  RPLWRCPHCSQSVCFLDIRIDQSMVKVLKEVGEDVSYVKISGDGSWQAVTENISNGNEHI 415

Query: 529  EKPKDAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAGESSAVNKNAAPHME 708
            EKP DAPSSNQET  Q+VDDIMDLTEN              PSPAGESS  N N APH +
Sbjct: 416  EKPNDAPSSNQETATQNVDDIMDLTENDDEMEKVDKDYDQMPSPAGESSLTNNNIAPHTD 475

Query: 709  DGFWTEYYS-----------------------------------MGLPGTRTNNSRSDVS 783
            DG+W  Y S                                    G   T T N+RSDV 
Sbjct: 476  DGYWRRYCSSALVTGTTNTRSDVFDAIQRQHLIFNSLGQSQGQGQGQSETGTTNTRSDVL 535

Query: 784  EVVQRNQQMFNSITLGQTQSQIPVSXXXXXXXXXXXXXXXXLYTRY---QNWVTRTPNAV 954
            +V+QR   +F+S+   Q QSQ+ VS                 + RY      +TR PNAV
Sbjct: 536  DVIQRQHLIFSSLGQSQGQSQMHVSNNTQSQHNENSNNNGNGHVRYPTSATLITRIPNAV 595

Query: 955  QALPAQSSATVANGDRQQHLSRPQLNQHQVSRMMSSQNQGQRDHPVPV---LPAQQHISN 1125
            QALPAQ+SA           S+P          +SSQ+Q Q   P+ V     + Q IS 
Sbjct: 596  QALPAQNSAITGQ-------SQPD------HSFLSSQSQSQSQTPLQVGARSASPQPISG 642

Query: 1126 GRH---IPSAHFNSYQQHSINQRPTSYPVRSPAHLSPQYRPVG---------PMTSSQNP 1269
             R+   + +  FNS Q  S+NQR    PVRS    SP  R  G          + SSQ+P
Sbjct: 643  SRYTSPLSTDRFNSNQYQSLNQR-AHLPVRSSGQPSPHVRLGGAHVGVSTMTSLASSQSP 701

Query: 1270 QYSMADVHRATSQATTHTSSIPTSIVTPGPSVTVASTGEQRGGSVDGP----GEENWRPT 1437
             Y    + R+TSQ  + +SS P           VA+T +QRG +VD P     EENWRP 
Sbjct: 702  TY----IQRSTSQIMSQSSSGP-----------VAATTDQRGSTVDAPPAAAAEENWRPA 746

Query: 1438 GRMRGSLSGRPYSETL-NQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMANNIR 1605
            GRMRGSLSG  Y+E L NQ II                TPRPFIPPHLQALMANN R
Sbjct: 747  GRMRGSLSGWQYTEALRNQFIIQPSEPVQAARPPPVLNTPRPFIPPHLQALMANNPR 803


>ref|XP_021969192.1| E4 SUMO-protein ligase PIAL2-like isoform X3 [Helianthus annuus]
          Length = 831

 Score =  525 bits (1353), Expect = e-175
 Identities = 311/599 (51%), Positives = 358/599 (59%), Gaps = 64/599 (10%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GV++RTC  KD  PQMPTPVT+MLKYGSNLLQVVGQF GRYI+ I FMSV+SNP+FPTIP
Sbjct: 236  GVDRRTCTYKDPAPQMPTPVTHMLKYGSNLLQVVGQFNGRYIIVIGFMSVVSNPSFPTIP 295

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            DY+PP+AA  DPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSC HLQCFDFNNY+GIN+R
Sbjct: 296  DYVPPIAAQSDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCNHLQCFDFNNYMGINSR 355

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTES----PENP 528
            RPLWRCPHCSQSV F DIRIDQSMVKVLKEVGED+SYVKI  DGSW++VTE+     E+ 
Sbjct: 356  RPLWRCPHCSQSVCFLDIRIDQSMVKVLKEVGEDVSYVKISGDGSWQAVTENISNGNEHI 415

Query: 529  EKPKDAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAGESSAVNKNAAPHME 708
            EKP DAPSSNQET  Q+VDDIMDLTEN              PSPAGESS  N N APH +
Sbjct: 416  EKPNDAPSSNQETATQNVDDIMDLTENDDEMEKVDKDYDQMPSPAGESSLTNNNIAPHTD 475

Query: 709  DGFWTEYYS-------------------------------------MGLPGTRTNNSRSD 777
            DG+W  Y S                                      G   T T N+RSD
Sbjct: 476  DGYWRRYCSSALVTGTTNTRSDVFDAIQRQHLIFNSLGQSQGQGQGQGQSETGTTNTRSD 535

Query: 778  VSEVVQRNQQMFNSITLGQTQSQIPVSXXXXXXXXXXXXXXXXLYTRY---QNWVTRTPN 948
            V +V+QR   +F+S+   Q QSQ+ VS                 + RY      +TR PN
Sbjct: 536  VLDVIQRQHLIFSSLGQSQGQSQMHVSNNTQSQHNENSNNNGNGHVRYPTSATLITRIPN 595

Query: 949  AVQALPAQSSATVANGDRQQHLSRPQLNQHQVSRMMSSQNQGQRDHPVPV---LPAQQHI 1119
            AVQALPAQ+SA           S+P          +SSQ+Q Q   P+ V     + Q I
Sbjct: 596  AVQALPAQNSAITGQ-------SQPD------HSFLSSQSQSQSQTPLQVGARSASPQPI 642

Query: 1120 SNGRH---IPSAHFNSYQQHSINQRPTSYPVRSPAHLSPQYRPVG---------PMTSSQ 1263
            S  R+   + +  FNS Q  S+NQR    PVRS    SP  R  G          + SSQ
Sbjct: 643  SGSRYTSPLSTDRFNSNQYQSLNQR-AHLPVRSSGQPSPHVRLGGAHVGVSTMTSLASSQ 701

Query: 1264 NPQYSMADVHRATSQATTHTSSIPTSIVTPGPSVTVASTGEQRGGSVDGP----GEENWR 1431
            +P Y    + R+TSQ  + +SS P           VA+T +QRG +VD P     EENWR
Sbjct: 702  SPTY----IQRSTSQIMSQSSSGP-----------VAATTDQRGSTVDAPPAAAAEENWR 746

Query: 1432 PTGRMRGSLSGRPYSETL-NQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMANNIR 1605
            P GRMRGSLSG  Y+E L NQ II                TPRPFIPPHLQALMANN R
Sbjct: 747  PAGRMRGSLSGWQYTEALRNQFIIQPSEPVQAARPPPVLNTPRPFIPPHLQALMANNPR 805


>ref|XP_021969191.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Helianthus annuus]
          Length = 831

 Score =  523 bits (1348), Expect = e-175
 Identities = 312/599 (52%), Positives = 359/599 (59%), Gaps = 64/599 (10%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GV++RTC  KD  PQMPTPVT+MLKYGSNLLQVVGQF GRYI+ I FMSV+SNP+FPTIP
Sbjct: 236  GVDRRTCTYKDPAPQMPTPVTHMLKYGSNLLQVVGQFNGRYIIVIGFMSVVSNPSFPTIP 295

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            DY+PP+AA  DPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSC HLQCFDFNNY+GIN+R
Sbjct: 296  DYVPPIAAQSDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCNHLQCFDFNNYMGINSR 355

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTES----PENP 528
            RPLWRCPHCSQSV F DIRIDQSMVKVLKEVGED+SYVKI  DGSW++VTE+     E+ 
Sbjct: 356  RPLWRCPHCSQSVCFLDIRIDQSMVKVLKEVGEDVSYVKISGDGSWQAVTENISNGNEHI 415

Query: 529  EKPKDAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAGESSAVNKNAAPHME 708
            EKP DAPSSNQET  Q+VDDIMDLTEN              PSPAGESS  N N APH +
Sbjct: 416  EKPNDAPSSNQETATQNVDDIMDLTENDDEMEKVDKDYDQMPSPAGESSLTNNNIAPHTD 475

Query: 709  DGFWTEYYS---------------------------------MGLPGTRTNNSRSDVSEV 789
            DG+W  Y S                                  G   T T N+RSDV + 
Sbjct: 476  DGYWRRYCSSALVTGTTNTRSDVFDAIQRQHLIFNSLGQSQGQGQSETGTANTRSDVLDA 535

Query: 790  VQRNQQMFNSI----TLGQTQSQIPVSXXXXXXXXXXXXXXXXLYTRY---QNWVTRTPN 948
            +QR + +FNS+      GQ QSQ+ VS                 + RY      +TR PN
Sbjct: 536  IQRQRLIFNSLGQSQGQGQGQSQMHVSNNTQSQHNENSNNNGNGHVRYPTSATLITRIPN 595

Query: 949  AVQALPAQSSATVANGDRQQHLSRPQLNQHQVSRMMSSQNQGQRDHPVPV---LPAQQHI 1119
            AVQALPAQ+SA           S+P          +SSQ+Q Q   P+ V     + Q I
Sbjct: 596  AVQALPAQNSAITGQ-------SQPD------HSFLSSQSQSQSQTPLQVGARSASPQPI 642

Query: 1120 SNGRH---IPSAHFNSYQQHSINQRPTSYPVRSPAHLSPQYRPVG---------PMTSSQ 1263
            S  R+   + +  FNS Q  S+NQR    PVRS    SP  R  G          + SSQ
Sbjct: 643  SGSRYTSPLSTDRFNSNQYQSLNQR-AHLPVRSSGQPSPHVRLGGAHVGVSTMTSLASSQ 701

Query: 1264 NPQYSMADVHRATSQATTHTSSIPTSIVTPGPSVTVASTGEQRGGSVDGP----GEENWR 1431
            +P Y    + R+TSQ  + +SS P           VA+T +QRG +VD P     EENWR
Sbjct: 702  SPTY----IQRSTSQIMSQSSSGP-----------VAATTDQRGSTVDAPPAAAAEENWR 746

Query: 1432 PTGRMRGSLSGRPYSETL-NQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMANNIR 1605
            P GRMRGSLSG  Y+E L NQ II                TPRPFIPPHLQALMANN R
Sbjct: 747  PAGRMRGSLSGWQYTEALRNQFIIQPSEPVQAARPPPVLNTPRPFIPPHLQALMANNPR 805


>gb|PLY70155.1| hypothetical protein LSAT_3X11501 [Lactuca sativa]
          Length = 750

 Score =  518 bits (1333), Expect = e-173
 Identities = 300/544 (55%), Positives = 348/544 (63%), Gaps = 9/544 (1%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GVE+RTC+ +D GPQ PTPVT+MLKYGSNLLQVVGQF GRYI+ IAFMSV+SNPT P I 
Sbjct: 227  GVERRTCIFRDPGPQAPTPVTHMLKYGSNLLQVVGQFNGRYIIVIAFMSVVSNPTCPPIL 286

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            DY+P  AA PD DNEIIEGPSR+SLKCPISFSRIKIPVKGH+CKHLQCFDFNNYVGIN R
Sbjct: 287  DYVPLAAAAPDSDNEIIEGPSRISLKCPISFSRIKIPVKGHTCKHLQCFDFNNYVGINTR 346

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPENPEKPK 540
            RP WRCPHCSQSV FHDIRIDQ M  VLKEVGED+S+VKI +DGSWE+VTES EN +K  
Sbjct: 347  RPQWRCPHCSQSVCFHDIRIDQRM--VLKEVGEDVSHVKISSDGSWEAVTESNENSDKQN 404

Query: 541  DAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAGESSAVNKNAAPHMEDGFW 720
            DAP  +Q+T IQ++DDIMDLTE               PS     + VNKN  PHMEDGFW
Sbjct: 405  DAPPVHQDTSIQNIDDIMDLTEG-----DNNEVDDKKPS----VNNVNKNIPPHMEDGFW 455

Query: 721  TEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQIPVSXXXXXXXXXXXXXX 900
             E+YS  LP TR  N+R D ++++   + +FNSI  GQ+QSQ                  
Sbjct: 456  REFYSPSLP-TRIANTRPDAADLIP-GRPIFNSIP-GQSQSQ-------SQSQNDNSNNG 505

Query: 901  XXLYTRYQNW---VTRTPNAVQALPAQSSATVANGDRQQHLSRPQLNQHQVSRMMSSQNQ 1071
              +++RY      VTR PN +QALPAQ+S+  A+ DRQQ      L   Q +RM S    
Sbjct: 506  SNVHSRYPTHMLNVTRAPNTIQALPAQTSSRSADVDRQQQNFSQILTNLQSARMSSQ--- 562

Query: 1072 GQRDHPVPVLPAQQHISNGRHIPSAHFNSYQQHSINQR-PTSYPVRSPAHLSPQYRP--- 1239
                  +P    QQ   N   +P   F S+   SINQR P   P+R  A   P  RP   
Sbjct: 563  ------IP----QQKGRNMSPLPPDRFWSHPPQSINQRTPVPVPMRPSAPTPPAARPPGG 612

Query: 1240 --VGPMTSSQNPQYSMADVHRATSQATTHTSSIPTSIVTPGPSVTVASTGEQRGGSVDGP 1413
              VGP   +    YS+AD+ RAT+     T S P SI TP PS+ V ST +QR       
Sbjct: 613  GGVGPNPMTSQSHYSLADIQRATN--VLRTPSTPVSIQTPAPSMPVTSTADQR--DPGSS 668

Query: 1414 GEENWRPTGRMRGSLSGRPYSETLNQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMA 1593
            G+ NWRPTGRMRGSLSGR YSE LNQ II                TPRPFIPPHLQALMA
Sbjct: 669  GDANWRPTGRMRGSLSGRAYSEALNQYII-QPNQPVQAARPPVLNTPRPFIPPHLQALMA 727

Query: 1594 NNIR 1605
            NN+R
Sbjct: 728  NNLR 731


>ref|XP_021969190.1| E4 SUMO-protein ligase PIAL2-like isoform X1 [Helianthus annuus]
 gb|OTG21955.1| putative zinc finger, MIZ-type, Zinc finger, RING/FYVE/PHD-type, E3
            SUMO protein ligase [Helianthus annuus]
          Length = 863

 Score =  494 bits (1272), Expect = e-163
 Identities = 292/603 (48%), Positives = 343/603 (56%), Gaps = 68/603 (11%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GV++RTC  KD  PQMPTPVT+MLKYGSNLLQVVGQF GRYI+ I FMSV+SNP+FPTIP
Sbjct: 236  GVDRRTCTYKDPAPQMPTPVTHMLKYGSNLLQVVGQFNGRYIIVIGFMSVVSNPSFPTIP 295

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            DY+PP+AA  DPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSC HLQCFDFNNY+GIN+R
Sbjct: 296  DYVPPIAAQSDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCNHLQCFDFNNYMGINSR 355

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTES----PENP 528
            RPLWRCPHCSQSV F DIRIDQSMVKVLKEVGED+SYVKI  DGSW++VTE+     E+ 
Sbjct: 356  RPLWRCPHCSQSVCFLDIRIDQSMVKVLKEVGEDVSYVKISGDGSWQAVTENISNGNEHI 415

Query: 529  EKPKDAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAGESSAVNKNAAPHME 708
            EKP DAPSSNQET  Q+VDDIMDLTEN              PSPAGESS  N N APH +
Sbjct: 416  EKPNDAPSSNQETATQNVDDIMDLTENDDEMEKVDKDYDQMPSPAGESSLTNNNIAPHTD 475

Query: 709  DGFWTEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQIPVSXXXXXXXXXX 888
            DG+W  Y S  L  T T N+RSDV + +QR   +FNS+   Q Q Q              
Sbjct: 476  DGYWRRYCSSALV-TGTTNTRSDVFDAIQRQHLIFNSLGQSQGQGQSETGTANTRSDVLD 534

Query: 889  XXXXXXL-YTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQLNQHQVSRMMSSQ 1065
                  L +        +     +     + + V +  ++QHL    L Q Q    M   
Sbjct: 535  AIQRQRLIFNSLGQSQGQGQGQSETGTTNTRSDVLDVIQRQHLIFSSLGQSQGQSQMHVS 594

Query: 1066 NQGQRDH--------------------------PVPVLPAQQHISNGRHIPSAHFNSYQQ 1167
            N  Q  H                           V  LPAQ     G+  P   F S Q 
Sbjct: 595  NNTQSQHNENSNNNGNGHVRYPTSATLITRIPNAVQALPAQNSAITGQSQPDHSFLSSQS 654

Query: 1168 HSINQRP--------TSYPVRSPAHLSP---------QYRPVG-----PMTSS--QNPQY 1275
             S +Q P        +  P+    + SP         QY+ +      P+ SS   +P  
Sbjct: 655  QSQSQTPLQVGARSASPQPISGSRYTSPLSTDRFNSNQYQSLNQRAHLPVRSSGQPSPHV 714

Query: 1276 SMADVH--------RATSQATTHTSSIPTSIVTPGPSVTVASTGEQRGGSVDGP----GE 1419
             +   H         A+SQ+ T+     + I++   S  VA+T +QRG +VD P     E
Sbjct: 715  RLGGAHVGVSTMTSLASSQSPTYIQRSTSQIMSQSSSGPVAATTDQRGSTVDAPPAAAAE 774

Query: 1420 ENWRPTGRMRGSLSGRPYSETL-NQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMAN 1596
            ENWRP GRMRGSLSG  Y+E L NQ II                TPRPFIPPHLQALMAN
Sbjct: 775  ENWRPAGRMRGSLSGWQYTEALRNQFIIQPSEPVQAARPPPVLNTPRPFIPPHLQALMAN 834

Query: 1597 NIR 1605
            N R
Sbjct: 835  NPR 837


>ref|XP_022002147.1| E4 SUMO-protein ligase PIAL2-like isoform X3 [Helianthus annuus]
          Length = 852

 Score =  355 bits (912), Expect = e-109
 Identities = 241/616 (39%), Positives = 307/616 (49%), Gaps = 83/616 (13%)
 Frame = +1

Query: 4    VEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIPD 183
            V++RTC+ KD GPQ+PTP+T+++K G+NLLQ VGQF G+YI+ +AFMSVI+NP+ P +PD
Sbjct: 224  VDRRTCISKDPGPQIPTPITDLVKNGTNLLQAVGQFNGKYIIVVAFMSVITNPSCPALPD 283

Query: 184  YIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINARR 363
            Y+PPVAA  D DN+I+EGPSR+SL CPISF RI  PVKGH CKHLQCFDF NYV IN+RR
Sbjct: 284  YMPPVAAASDLDNDIVEGPSRISLNCPISFKRITTPVKGHLCKHLQCFDFENYVDINSRR 343

Query: 364  PLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPENPEKPKD 543
            P WRCPHCSQSV F DIRIDQ M KVLK+VG  IS+V    DGSWE+  E  ++ +K +D
Sbjct: 344  PSWRCPHCSQSVCFTDIRIDQVMAKVLKDVGGTISHVNFSADGSWETFNECDDHADKQQD 403

Query: 544  APSSNQETYIQDV-DDIMDLTE----NXXXXXXXXXXXXXXPSPA--------GESS--- 675
                 Q     +   DIMDLT+    N              PS A        G S    
Sbjct: 404  KSLHQQSNPSTNTGGDIMDLTKGDTGNDIDASNPHQENEKKPSLAQLQNQNECGTSDTRS 463

Query: 676  --AVNKNAAPHMEDGFWTEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQI 849
              AV++  +  +  GF      + LP      S +  S  +Q   Q  NS    Q Q   
Sbjct: 464  NVAVSQGQSQTLMSGF--NNMQLPLPTHAIGTSSTVTSPNLQGQSQTENSAYSMQYQQHN 521

Query: 850  PVSXXXXXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQL 1029
              S                 Y      VTR P A+Q LPAQ+ A + N DRQQ  SR  +
Sbjct: 522  MNSMSNGYGQR---------YMTPSRQVTRIPIAIQGLPAQTPAGMVNTDRQQQYSRFYM 572

Query: 1030 NQHQVSRMMSSQNQGQRDH------------PVPVLPAQQ---HISNGRHIPSAHFNSYQ 1164
            +Q+Q  R +++ N    ++             V   PAQQ   H          HFNS+Q
Sbjct: 573  DQYQGMRTIAASNPSVSENIGSQNWVRHGLSNVSSHPAQQFGAHSGPFYQSDGHHFNSHQ 632

Query: 1165 QHSI-------------------NQRPTSYPVRSPAHLSPQY------------------ 1233
            Q S                    NQ+   Y   +   +S Q                   
Sbjct: 633  QQSYNLPNLVQPPFVGPTIGQVSNQQAHQYVDPTTGSISNQQAHHYVGPTTGSVSNQQTR 692

Query: 1234 RPVGPMTSSQNPQYSMADVHRATSQATTHTSS-IPTSIVTPGPSVTVASTGEQRGG---- 1398
              V PMT S + Q +   V  A SQA+  T    P  + T   ++ V S+ EQRGG    
Sbjct: 693  HYVPPMTGSISNQLAHQYVKAAASQASQITGGPTPAPVQTSLSTMPVISSEEQRGGLGQA 752

Query: 1399 --------SVDGPGEENWRPTGRMRGSLSGRPYSETLNQNIIXXXXXXXXXXXXXXXXTP 1554
                      +   + NWRP+ RMRGSLSGR YSE L+Q ++                TP
Sbjct: 753  FRTQEGSALANALADPNWRPSVRMRGSLSGRAYSEALSQAMV-HPTQPAQAASPLVLNTP 811

Query: 1555 RPFIPPHLQALMANNI 1602
            RPFIPPHLQ L+ANN+
Sbjct: 812  RPFIPPHLQVLIANNM 827


>ref|XP_022002150.1| E4 SUMO-protein ligase PIAL2-like isoform X6 [Helianthus annuus]
          Length = 789

 Score =  342 bits (878), Expect = e-105
 Identities = 243/648 (37%), Positives = 311/648 (47%), Gaps = 115/648 (17%)
 Frame = +1

Query: 4    VEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIPD 183
            V++RTC+ KD GPQ+PTP+T+++K G+NLLQ VGQF G+YI+ +AFMSVI+NP+ P +PD
Sbjct: 129  VDRRTCISKDPGPQIPTPITDLVKNGTNLLQAVGQFNGKYIIVVAFMSVITNPSCPALPD 188

Query: 184  YIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINARR 363
            Y+PPVAA  D DN+I+EGPSR+SL CPISF RI  PVKGH CKHLQCFDF NYV IN+RR
Sbjct: 189  YMPPVAAASDLDNDIVEGPSRISLNCPISFKRITTPVKGHLCKHLQCFDFENYVDINSRR 248

Query: 364  PLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPENPEKPKD 543
            P WRCPHCSQSV F DIRIDQ M KVLK+VG  IS+V    DGSWE+  E  ++ +K +D
Sbjct: 249  PSWRCPHCSQSVCFTDIRIDQVMAKVLKDVGGTISHVNFSADGSWETFNECDDHADKQQD 308

Query: 544  APSSNQETYIQDV-DDIMDLTE----NXXXXXXXXXXXXXXPSPA--------GESS--- 675
                 Q     +   DIMDLT+    N              PS A        G S    
Sbjct: 309  KSLHQQSNPSTNTGGDIMDLTKGDTGNDIDASNPHQENEKKPSLAQLQNQNECGTSDTRS 368

Query: 676  --AVNKNAAPHMEDGFWTEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQI 849
              AV++  +  +  GF      + LP      S +  S  +Q   Q  NS    Q Q   
Sbjct: 369  NVAVSQGQSQTLMSGF--NNMQLPLPTHAIGTSSTVTSPNLQGQSQTENSAYSMQYQQHN 426

Query: 850  PVSXXXXXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQL 1029
              S                 Y      VTR P A+Q LPAQ+ A + N DRQQ  SR  +
Sbjct: 427  MNSMSNGYGQR---------YMTPSRQVTRIPIAIQGLPAQTPAGMVNTDRQQQYSRFYM 477

Query: 1030 NQHQVSRMMSSQNQGQRDH------------PVPVLPAQQ---HISNGRHIPSAHFNSYQ 1164
            +Q+Q  R +++ N    ++             V   PAQQ   H          HFNS+Q
Sbjct: 478  DQYQGMRTIAASNPSVSENIGSQNWVRHGLSNVSSHPAQQFGAHSGPFYQSDGHHFNSHQ 537

Query: 1165 QHSI-------------------NQRPTSYPVRSPAHLSPQ--YRPVGPMTSSQNPQYSM 1281
            Q S                    NQ+   Y   +   +S Q  +  VGP   S + Q + 
Sbjct: 538  QQSYNLPNLVQPPFVGPTIGQVSNQQAHQYVDPTTGSISNQQAHHYVGPTAGSVSNQQAH 597

Query: 1282 ADVHRAT--------------------SQATTH-----TSSIPTSI-------------- 1344
              V   T                    +Q T H     T SI   +              
Sbjct: 598  QYVDPTTGSISNQQAHHYVGPTTGSVSNQQTRHYVPPMTGSISNQLAHQYVKAAASQASQ 657

Query: 1345 VTPGP----------SVTVASTGEQRGG------------SVDGPGEENWRPTGRMRGSL 1458
            +T GP          ++ V S+ EQRGG              +   + NWRP+ RMRGSL
Sbjct: 658  ITGGPTPAPVQTSLSTMPVISSEEQRGGLGQAFRTQEGSALANALADPNWRPSVRMRGSL 717

Query: 1459 SGRPYSETLNQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMANNI 1602
            SGR YSE L+Q ++                TPRPFIPPHLQ L+ANN+
Sbjct: 718  SGRAYSEALSQAMV-HPTQPAQAASPLVLNTPRPFIPPHLQVLIANNM 764


>ref|XP_022002149.1| E4 SUMO-protein ligase PIAL2-like isoform X5 [Helianthus annuus]
          Length = 832

 Score =  342 bits (878), Expect = e-105
 Identities = 243/648 (37%), Positives = 311/648 (47%), Gaps = 115/648 (17%)
 Frame = +1

Query: 4    VEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIPD 183
            V++RTC+ KD GPQ+PTP+T+++K G+NLLQ VGQF G+YI+ +AFMSVI+NP+ P +PD
Sbjct: 172  VDRRTCISKDPGPQIPTPITDLVKNGTNLLQAVGQFNGKYIIVVAFMSVITNPSCPALPD 231

Query: 184  YIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINARR 363
            Y+PPVAA  D DN+I+EGPSR+SL CPISF RI  PVKGH CKHLQCFDF NYV IN+RR
Sbjct: 232  YMPPVAAASDLDNDIVEGPSRISLNCPISFKRITTPVKGHLCKHLQCFDFENYVDINSRR 291

Query: 364  PLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPENPEKPKD 543
            P WRCPHCSQSV F DIRIDQ M KVLK+VG  IS+V    DGSWE+  E  ++ +K +D
Sbjct: 292  PSWRCPHCSQSVCFTDIRIDQVMAKVLKDVGGTISHVNFSADGSWETFNECDDHADKQQD 351

Query: 544  APSSNQETYIQDV-DDIMDLTE----NXXXXXXXXXXXXXXPSPA--------GESS--- 675
                 Q     +   DIMDLT+    N              PS A        G S    
Sbjct: 352  KSLHQQSNPSTNTGGDIMDLTKGDTGNDIDASNPHQENEKKPSLAQLQNQNECGTSDTRS 411

Query: 676  --AVNKNAAPHMEDGFWTEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQI 849
              AV++  +  +  GF      + LP      S +  S  +Q   Q  NS    Q Q   
Sbjct: 412  NVAVSQGQSQTLMSGF--NNMQLPLPTHAIGTSSTVTSPNLQGQSQTENSAYSMQYQQHN 469

Query: 850  PVSXXXXXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQL 1029
              S                 Y      VTR P A+Q LPAQ+ A + N DRQQ  SR  +
Sbjct: 470  MNSMSNGYGQR---------YMTPSRQVTRIPIAIQGLPAQTPAGMVNTDRQQQYSRFYM 520

Query: 1030 NQHQVSRMMSSQNQGQRDH------------PVPVLPAQQ---HISNGRHIPSAHFNSYQ 1164
            +Q+Q  R +++ N    ++             V   PAQQ   H          HFNS+Q
Sbjct: 521  DQYQGMRTIAASNPSVSENIGSQNWVRHGLSNVSSHPAQQFGAHSGPFYQSDGHHFNSHQ 580

Query: 1165 QHSI-------------------NQRPTSYPVRSPAHLSPQ--YRPVGPMTSSQNPQYSM 1281
            Q S                    NQ+   Y   +   +S Q  +  VGP   S + Q + 
Sbjct: 581  QQSYNLPNLVQPPFVGPTIGQVSNQQAHQYVDPTTGSISNQQAHHYVGPTAGSVSNQQAH 640

Query: 1282 ADVHRAT--------------------SQATTH-----TSSIPTSI-------------- 1344
              V   T                    +Q T H     T SI   +              
Sbjct: 641  QYVDPTTGSISNQQAHHYVGPTTGSVSNQQTRHYVPPMTGSISNQLAHQYVKAAASQASQ 700

Query: 1345 VTPGP----------SVTVASTGEQRGG------------SVDGPGEENWRPTGRMRGSL 1458
            +T GP          ++ V S+ EQRGG              +   + NWRP+ RMRGSL
Sbjct: 701  ITGGPTPAPVQTSLSTMPVISSEEQRGGLGQAFRTQEGSALANALADPNWRPSVRMRGSL 760

Query: 1459 SGRPYSETLNQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMANNI 1602
            SGR YSE L+Q ++                TPRPFIPPHLQ L+ANN+
Sbjct: 761  SGRAYSEALSQAMV-HPTQPAQAASPLVLNTPRPFIPPHLQVLIANNM 807


>ref|XP_022002148.1| E4 SUMO-protein ligase PIAL2-like isoform X4 [Helianthus annuus]
          Length = 836

 Score =  342 bits (878), Expect = e-105
 Identities = 243/648 (37%), Positives = 311/648 (47%), Gaps = 115/648 (17%)
 Frame = +1

Query: 4    VEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIPD 183
            V++RTC+ KD GPQ+PTP+T+++K G+NLLQ VGQF G+YI+ +AFMSVI+NP+ P +PD
Sbjct: 176  VDRRTCISKDPGPQIPTPITDLVKNGTNLLQAVGQFNGKYIIVVAFMSVITNPSCPALPD 235

Query: 184  YIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINARR 363
            Y+PPVAA  D DN+I+EGPSR+SL CPISF RI  PVKGH CKHLQCFDF NYV IN+RR
Sbjct: 236  YMPPVAAASDLDNDIVEGPSRISLNCPISFKRITTPVKGHLCKHLQCFDFENYVDINSRR 295

Query: 364  PLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPENPEKPKD 543
            P WRCPHCSQSV F DIRIDQ M KVLK+VG  IS+V    DGSWE+  E  ++ +K +D
Sbjct: 296  PSWRCPHCSQSVCFTDIRIDQVMAKVLKDVGGTISHVNFSADGSWETFNECDDHADKQQD 355

Query: 544  APSSNQETYIQDV-DDIMDLTE----NXXXXXXXXXXXXXXPSPA--------GESS--- 675
                 Q     +   DIMDLT+    N              PS A        G S    
Sbjct: 356  KSLHQQSNPSTNTGGDIMDLTKGDTGNDIDASNPHQENEKKPSLAQLQNQNECGTSDTRS 415

Query: 676  --AVNKNAAPHMEDGFWTEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQI 849
              AV++  +  +  GF      + LP      S +  S  +Q   Q  NS    Q Q   
Sbjct: 416  NVAVSQGQSQTLMSGF--NNMQLPLPTHAIGTSSTVTSPNLQGQSQTENSAYSMQYQQHN 473

Query: 850  PVSXXXXXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQL 1029
              S                 Y      VTR P A+Q LPAQ+ A + N DRQQ  SR  +
Sbjct: 474  MNSMSNGYGQR---------YMTPSRQVTRIPIAIQGLPAQTPAGMVNTDRQQQYSRFYM 524

Query: 1030 NQHQVSRMMSSQNQGQRDH------------PVPVLPAQQ---HISNGRHIPSAHFNSYQ 1164
            +Q+Q  R +++ N    ++             V   PAQQ   H          HFNS+Q
Sbjct: 525  DQYQGMRTIAASNPSVSENIGSQNWVRHGLSNVSSHPAQQFGAHSGPFYQSDGHHFNSHQ 584

Query: 1165 QHSI-------------------NQRPTSYPVRSPAHLSPQ--YRPVGPMTSSQNPQYSM 1281
            Q S                    NQ+   Y   +   +S Q  +  VGP   S + Q + 
Sbjct: 585  QQSYNLPNLVQPPFVGPTIGQVSNQQAHQYVDPTTGSISNQQAHHYVGPTAGSVSNQQAH 644

Query: 1282 ADVHRAT--------------------SQATTH-----TSSIPTSI-------------- 1344
              V   T                    +Q T H     T SI   +              
Sbjct: 645  QYVDPTTGSISNQQAHHYVGPTTGSVSNQQTRHYVPPMTGSISNQLAHQYVKAAASQASQ 704

Query: 1345 VTPGP----------SVTVASTGEQRGG------------SVDGPGEENWRPTGRMRGSL 1458
            +T GP          ++ V S+ EQRGG              +   + NWRP+ RMRGSL
Sbjct: 705  ITGGPTPAPVQTSLSTMPVISSEEQRGGLGQAFRTQEGSALANALADPNWRPSVRMRGSL 764

Query: 1459 SGRPYSETLNQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMANNI 1602
            SGR YSE L+Q ++                TPRPFIPPHLQ L+ANN+
Sbjct: 765  SGRAYSEALSQAMV-HPTQPAQAASPLVLNTPRPFIPPHLQVLIANNM 811


>ref|XP_022002145.1| E4 SUMO-protein ligase PIAL2-like isoform X1 [Helianthus annuus]
          Length = 884

 Score =  342 bits (878), Expect = e-104
 Identities = 243/648 (37%), Positives = 311/648 (47%), Gaps = 115/648 (17%)
 Frame = +1

Query: 4    VEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIPD 183
            V++RTC+ KD GPQ+PTP+T+++K G+NLLQ VGQF G+YI+ +AFMSVI+NP+ P +PD
Sbjct: 224  VDRRTCISKDPGPQIPTPITDLVKNGTNLLQAVGQFNGKYIIVVAFMSVITNPSCPALPD 283

Query: 184  YIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINARR 363
            Y+PPVAA  D DN+I+EGPSR+SL CPISF RI  PVKGH CKHLQCFDF NYV IN+RR
Sbjct: 284  YMPPVAAASDLDNDIVEGPSRISLNCPISFKRITTPVKGHLCKHLQCFDFENYVDINSRR 343

Query: 364  PLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPENPEKPKD 543
            P WRCPHCSQSV F DIRIDQ M KVLK+VG  IS+V    DGSWE+  E  ++ +K +D
Sbjct: 344  PSWRCPHCSQSVCFTDIRIDQVMAKVLKDVGGTISHVNFSADGSWETFNECDDHADKQQD 403

Query: 544  APSSNQETYIQDV-DDIMDLTE----NXXXXXXXXXXXXXXPSPA--------GESS--- 675
                 Q     +   DIMDLT+    N              PS A        G S    
Sbjct: 404  KSLHQQSNPSTNTGGDIMDLTKGDTGNDIDASNPHQENEKKPSLAQLQNQNECGTSDTRS 463

Query: 676  --AVNKNAAPHMEDGFWTEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQI 849
              AV++  +  +  GF      + LP      S +  S  +Q   Q  NS    Q Q   
Sbjct: 464  NVAVSQGQSQTLMSGF--NNMQLPLPTHAIGTSSTVTSPNLQGQSQTENSAYSMQYQQHN 521

Query: 850  PVSXXXXXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQL 1029
              S                 Y      VTR P A+Q LPAQ+ A + N DRQQ  SR  +
Sbjct: 522  MNSMSNGYGQR---------YMTPSRQVTRIPIAIQGLPAQTPAGMVNTDRQQQYSRFYM 572

Query: 1030 NQHQVSRMMSSQNQGQRDH------------PVPVLPAQQ---HISNGRHIPSAHFNSYQ 1164
            +Q+Q  R +++ N    ++             V   PAQQ   H          HFNS+Q
Sbjct: 573  DQYQGMRTIAASNPSVSENIGSQNWVRHGLSNVSSHPAQQFGAHSGPFYQSDGHHFNSHQ 632

Query: 1165 QHSI-------------------NQRPTSYPVRSPAHLSPQ--YRPVGPMTSSQNPQYSM 1281
            Q S                    NQ+   Y   +   +S Q  +  VGP   S + Q + 
Sbjct: 633  QQSYNLPNLVQPPFVGPTIGQVSNQQAHQYVDPTTGSISNQQAHHYVGPTAGSVSNQQAH 692

Query: 1282 ADVHRAT--------------------SQATTH-----TSSIPTSI-------------- 1344
              V   T                    +Q T H     T SI   +              
Sbjct: 693  QYVDPTTGSISNQQAHHYVGPTTGSVSNQQTRHYVPPMTGSISNQLAHQYVKAAASQASQ 752

Query: 1345 VTPGP----------SVTVASTGEQRGG------------SVDGPGEENWRPTGRMRGSL 1458
            +T GP          ++ V S+ EQRGG              +   + NWRP+ RMRGSL
Sbjct: 753  ITGGPTPAPVQTSLSTMPVISSEEQRGGLGQAFRTQEGSALANALADPNWRPSVRMRGSL 812

Query: 1459 SGRPYSETLNQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMANNI 1602
            SGR YSE L+Q ++                TPRPFIPPHLQ L+ANN+
Sbjct: 813  SGRAYSEALSQAMV-HPTQPAQAASPLVLNTPRPFIPPHLQVLIANNM 859


>ref|XP_022002146.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Helianthus annuus]
          Length = 883

 Score =  341 bits (874), Expect = e-104
 Identities = 244/647 (37%), Positives = 312/647 (48%), Gaps = 114/647 (17%)
 Frame = +1

Query: 4    VEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIPD 183
            V++RTC+ KD GPQ+PTP+T+++K G+NLLQ VGQF G+YI+ +AFMSVI+NP+ P +PD
Sbjct: 224  VDRRTCISKDPGPQIPTPITDLVKNGTNLLQAVGQFNGKYIIVVAFMSVITNPSCPALPD 283

Query: 184  YIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINARR 363
            Y+PPVAA  D DN+I+EGPSR+SL CPISF RI  PVKGH CKHLQCFDF NYV IN+RR
Sbjct: 284  YMPPVAAASDLDNDIVEGPSRISLNCPISFKRITTPVKGHLCKHLQCFDFENYVDINSRR 343

Query: 364  PLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPENPEKPKD 543
            P WRCPHCSQSV F DIRIDQ M KVLK+VG  IS+V    DGSWE+  E  ++ +K +D
Sbjct: 344  PSWRCPHCSQSVCFTDIRIDQVMAKVLKDVGGTISHVNFSADGSWETFNECDDHADKQQD 403

Query: 544  APSSNQETYIQDV-DDIMDLTE----NXXXXXXXXXXXXXXPSPA--------GESS--- 675
                 Q     +   DIMDLT+    N              PS A        G S    
Sbjct: 404  KSLHQQSNPSTNTGGDIMDLTKGDTGNDIDASNPHQENEKKPSLAQLQNQNECGTSDTRS 463

Query: 676  --AVNKNAAPHMEDGFWTEYYSMGLPGTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQI 849
              AV++  +  +  GF      + LP      S +  S  +Q   Q  NS    Q Q   
Sbjct: 464  NVAVSQGQSQTLMSGF--NNMQLPLPTHAIGTSSTVTSPNLQGQSQTENSAYSMQYQQHN 521

Query: 850  PVSXXXXXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQL 1029
              S                 Y      VTR P A+Q LPAQ+ A + N DRQQ  SR  +
Sbjct: 522  MNSMSNGYGQR---------YMTPSRQVTRIPIAIQGLPAQTPAGMVNTDRQQQYSRFYM 572

Query: 1030 NQHQVSRMMSSQNQGQRDH------------PVPVLPAQQ---HISNGRHIPSAHFNSYQ 1164
            +Q+Q  R +++ N    ++             V   PAQQ   H          HFNS+Q
Sbjct: 573  DQYQGMRTIAASNPSVSENIGSQNWVRHGLSNVSSHPAQQFGAHSGPFYQSDGHHFNSHQ 632

Query: 1165 QHSI-------------------NQRPTSYPVRSPAHLSPQ--YRPVGPMTSSQNPQYSM 1281
            Q S                    NQ+   Y   +   +S Q  +  VGP   S + Q + 
Sbjct: 633  QQSYNLPNLVQPPFVGPTIGQVSNQQAHQYVDPTTGSISNQQAHHYVGPTAGSVSNQQAH 692

Query: 1282 ADVHRAT--------------------SQATTH-----TSSIPTSI-------------- 1344
              V   T                    +Q T H     T SI   +              
Sbjct: 693  QYVDPTTGSISNQQAHHYVGPTTGSVSNQQTRHYVPPMTGSISNQLAHQYVKAAASQASQ 752

Query: 1345 VTPGP----------SVTVASTGEQRG---------GS--VDGPGEENWRPTGRMRGSLS 1461
            +T GP          ++ V S+ EQRG         GS   +   + NWRP+ RMRGSLS
Sbjct: 753  ITGGPTPAPVQTSLSTMPVISSEEQRGLGQAFRTQEGSALANALADPNWRPSVRMRGSLS 812

Query: 1462 GRPYSETLNQNIIXXXXXXXXXXXXXXXXTPRPFIPPHLQALMANNI 1602
            GR YSE L+Q ++                TPRPFIPPHLQ L+ANN+
Sbjct: 813  GRAYSEALSQAMV-HPTQPAQAASPLVLNTPRPFIPPHLQVLIANNM 858


>ref|XP_023752744.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Lactuca sativa]
          Length = 664

 Score =  334 bits (856), Expect = e-103
 Identities = 227/557 (40%), Positives = 289/557 (51%), Gaps = 25/557 (4%)
 Frame = +1

Query: 4    VEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIPD 183
            V  RTCV KD GPQ PT VT+MLK G+NLLQVVGQF G YI+ +AFMS+ISNP  PT+P+
Sbjct: 129  VNGRTCVSKDPGPQTPTLVTHMLKLGTNLLQVVGQFNGNYIIIVAFMSMISNPISPTLPN 188

Query: 184  YIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINARR 363
            Y+PPVAA PDPDNE+ EGPS  SL CPISF RIK PVKG  CKH QCFD++NYV IN+RR
Sbjct: 189  YVPPVAAAPDPDNELTEGPSTTSLNCPISFKRIKTPVKGQLCKHRQCFDYDNYVDINSRR 248

Query: 364  PLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPE------N 525
            P W+CPHCSQSV F DIRID+ MVKVLKEVG ++S+V+I  DGSWE+V E  +      N
Sbjct: 249  PSWKCPHCSQSVCFTDIRIDRIMVKVLKEVGVNVSHVRISADGSWEAVKEEDDHNTDNNN 308

Query: 526  PEKPKDAPSSNQETYIQDVDDIMDLTE-----NXXXXXXXXXXXXXXPSPAGESSAVNKN 690
              K +  P  +Q         I+DLTE     N              PSP   S   N N
Sbjct: 309  NNKLQSTPPLHQNM----GPGILDLTEGDNYINATATDPHHQNIDKKPSP---SQLQNPN 361

Query: 691  AAPHMEDGFWTEYYSMGLP--GTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQIPVSXX 864
            +         T   ++ LP  G +   + + +   VQ N  +       Q QSQ+  S  
Sbjct: 362  S---------TNIRNIPLPPNGFQKIQTSNTMVPPVQMNPIL-------QGQSQLSASNT 405

Query: 865  XXXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQLNQHQV 1044
                            +R+   +TR P AVQALPAQ+S    +G     L    +N HQ+
Sbjct: 406  MQSKDVNGIVRYPTHSSRH---ITRIPIAVQALPAQTSVGPGSGG----LDGFYMNPHQI 458

Query: 1045 SRMMSSQNQGQRDHPVPVLPAQQHISNGRHIPSAHFNSYQQHSINQRPTSYPVRSPAHLS 1224
            +R +S             +P  QH+          +NS+QQH   Q        +   LS
Sbjct: 459  NRTVS-------------VPPSQHMGPQAFDGGYQYNSHQQHQSPQVQQVGVGPTNGILS 505

Query: 1225 PQYRPVGPMT-SSQNPQYSMADVHRATSQATTHTSSIPTS----IVTPGPSVTVASTGEQ 1389
             Q      M+ S+Q    + A V+   +QA  H  ++  +    +     +   A +  Q
Sbjct: 506  NQQNYAMSMSMSAQQQAATNAHVNNQLAQAQAHHYAMSAATNGHVSNNQQARHHAMSAAQ 565

Query: 1390 RG-------GSVDGPGEENWRPTGRMRGSLSGRPYSETLNQNIIXXXXXXXXXXXXXXXX 1548
            +G        S    G++NWRP+GRMRGSLSG+ YSE LNQ  I                
Sbjct: 566  QGFHVRSWTPSSHDHGDQNWRPSGRMRGSLSGQAYSEALNQ-YIYLPTQPVQATRPPLLN 624

Query: 1549 TPRPFIPPHLQALMANN 1599
            TPRP IPPHLQ L ANN
Sbjct: 625  TPRPVIPPHLQFLFANN 641


>ref|XP_023752743.1| E4 SUMO-protein ligase PIAL2-like isoform X1 [Lactuca sativa]
          Length = 766

 Score =  334 bits (856), Expect = e-102
 Identities = 227/557 (40%), Positives = 289/557 (51%), Gaps = 25/557 (4%)
 Frame = +1

Query: 4    VEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIPD 183
            V  RTCV KD GPQ PT VT+MLK G+NLLQVVGQF G YI+ +AFMS+ISNP  PT+P+
Sbjct: 231  VNGRTCVSKDPGPQTPTLVTHMLKLGTNLLQVVGQFNGNYIIIVAFMSMISNPISPTLPN 290

Query: 184  YIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINARR 363
            Y+PPVAA PDPDNE+ EGPS  SL CPISF RIK PVKG  CKH QCFD++NYV IN+RR
Sbjct: 291  YVPPVAAAPDPDNELTEGPSTTSLNCPISFKRIKTPVKGQLCKHRQCFDYDNYVDINSRR 350

Query: 364  PLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPE------N 525
            P W+CPHCSQSV F DIRID+ MVKVLKEVG ++S+V+I  DGSWE+V E  +      N
Sbjct: 351  PSWKCPHCSQSVCFTDIRIDRIMVKVLKEVGVNVSHVRISADGSWEAVKEEDDHNTDNNN 410

Query: 526  PEKPKDAPSSNQETYIQDVDDIMDLTE-----NXXXXXXXXXXXXXXPSPAGESSAVNKN 690
              K +  P  +Q         I+DLTE     N              PSP   S   N N
Sbjct: 411  NNKLQSTPPLHQNM----GPGILDLTEGDNYINATATDPHHQNIDKKPSP---SQLQNPN 463

Query: 691  AAPHMEDGFWTEYYSMGLP--GTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQIPVSXX 864
            +         T   ++ LP  G +   + + +   VQ N  +       Q QSQ+  S  
Sbjct: 464  S---------TNIRNIPLPPNGFQKIQTSNTMVPPVQMNPIL-------QGQSQLSASNT 507

Query: 865  XXXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQLNQHQV 1044
                            +R+   +TR P AVQALPAQ+S    +G     L    +N HQ+
Sbjct: 508  MQSKDVNGIVRYPTHSSRH---ITRIPIAVQALPAQTSVGPGSGG----LDGFYMNPHQI 560

Query: 1045 SRMMSSQNQGQRDHPVPVLPAQQHISNGRHIPSAHFNSYQQHSINQRPTSYPVRSPAHLS 1224
            +R +S             +P  QH+          +NS+QQH   Q        +   LS
Sbjct: 561  NRTVS-------------VPPSQHMGPQAFDGGYQYNSHQQHQSPQVQQVGVGPTNGILS 607

Query: 1225 PQYRPVGPMT-SSQNPQYSMADVHRATSQATTHTSSIPTS----IVTPGPSVTVASTGEQ 1389
             Q      M+ S+Q    + A V+   +QA  H  ++  +    +     +   A +  Q
Sbjct: 608  NQQNYAMSMSMSAQQQAATNAHVNNQLAQAQAHHYAMSAATNGHVSNNQQARHHAMSAAQ 667

Query: 1390 RG-------GSVDGPGEENWRPTGRMRGSLSGRPYSETLNQNIIXXXXXXXXXXXXXXXX 1548
            +G        S    G++NWRP+GRMRGSLSG+ YSE LNQ  I                
Sbjct: 668  QGFHVRSWTPSSHDHGDQNWRPSGRMRGSLSGQAYSEALNQ-YIYLPTQPVQATRPPLLN 726

Query: 1549 TPRPFIPPHLQALMANN 1599
            TPRP IPPHLQ L ANN
Sbjct: 727  TPRPVIPPHLQFLFANN 743


>gb|PLY93837.1| hypothetical protein LSAT_6X84940 [Lactuca sativa]
          Length = 767

 Score =  334 bits (856), Expect = e-102
 Identities = 227/557 (40%), Positives = 289/557 (51%), Gaps = 25/557 (4%)
 Frame = +1

Query: 4    VEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIPD 183
            V  RTCV KD GPQ PT VT+MLK G+NLLQVVGQF G YI+ +AFMS+ISNP  PT+P+
Sbjct: 232  VNGRTCVSKDPGPQTPTLVTHMLKLGTNLLQVVGQFNGNYIIIVAFMSMISNPISPTLPN 291

Query: 184  YIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINARR 363
            Y+PPVAA PDPDNE+ EGPS  SL CPISF RIK PVKG  CKH QCFD++NYV IN+RR
Sbjct: 292  YVPPVAAAPDPDNELTEGPSTTSLNCPISFKRIKTPVKGQLCKHRQCFDYDNYVDINSRR 351

Query: 364  PLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTESPE------N 525
            P W+CPHCSQSV F DIRID+ MVKVLKEVG ++S+V+I  DGSWE+V E  +      N
Sbjct: 352  PSWKCPHCSQSVCFTDIRIDRIMVKVLKEVGVNVSHVRISADGSWEAVKEEDDHNTDNNN 411

Query: 526  PEKPKDAPSSNQETYIQDVDDIMDLTE-----NXXXXXXXXXXXXXXPSPAGESSAVNKN 690
              K +  P  +Q         I+DLTE     N              PSP   S   N N
Sbjct: 412  NNKLQSTPPLHQNM----GPGILDLTEGDNYINATATDPHHQNIDKKPSP---SQLQNPN 464

Query: 691  AAPHMEDGFWTEYYSMGLP--GTRTNNSRSDVSEVVQRNQQMFNSITLGQTQSQIPVSXX 864
            +         T   ++ LP  G +   + + +   VQ N  +       Q QSQ+  S  
Sbjct: 465  S---------TNIRNIPLPPNGFQKIQTSNTMVPPVQMNPIL-------QGQSQLSASNT 508

Query: 865  XXXXXXXXXXXXXXLYTRYQNWVTRTPNAVQALPAQSSATVANGDRQQHLSRPQLNQHQV 1044
                            +R+   +TR P AVQALPAQ+S    +G     L    +N HQ+
Sbjct: 509  MQSKDVNGIVRYPTHSSRH---ITRIPIAVQALPAQTSVGPGSGG----LDGFYMNPHQI 561

Query: 1045 SRMMSSQNQGQRDHPVPVLPAQQHISNGRHIPSAHFNSYQQHSINQRPTSYPVRSPAHLS 1224
            +R +S             +P  QH+          +NS+QQH   Q        +   LS
Sbjct: 562  NRTVS-------------VPPSQHMGPQAFDGGYQYNSHQQHQSPQVQQVGVGPTNGILS 608

Query: 1225 PQYRPVGPMT-SSQNPQYSMADVHRATSQATTHTSSIPTS----IVTPGPSVTVASTGEQ 1389
             Q      M+ S+Q    + A V+   +QA  H  ++  +    +     +   A +  Q
Sbjct: 609  NQQNYAMSMSMSAQQQAATNAHVNNQLAQAQAHHYAMSAATNGHVSNNQQARHHAMSAAQ 668

Query: 1390 RG-------GSVDGPGEENWRPTGRMRGSLSGRPYSETLNQNIIXXXXXXXXXXXXXXXX 1548
            +G        S    G++NWRP+GRMRGSLSG+ YSE LNQ  I                
Sbjct: 669  QGFHVRSWTPSSHDHGDQNWRPSGRMRGSLSGQAYSEALNQ-YIYLPTQPVQATRPPLLN 727

Query: 1549 TPRPFIPPHLQALMANN 1599
            TPRP IPPHLQ L ANN
Sbjct: 728  TPRPVIPPHLQFLFANN 744


>ref|XP_019151668.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X1 [Ipomoea nil]
          Length = 894

 Score =  324 bits (831), Expect = 2e-97
 Identities = 242/641 (37%), Positives = 312/641 (48%), Gaps = 109/641 (17%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GVEKRT V  DTGPQ+PT V++ LKYG+NLLQ VG+F G YIVA+AFMS++  P   T+ 
Sbjct: 233  GVEKRTNVLMDTGPQIPTIVSHTLKYGTNLLQAVGEFNGNYIVAVAFMSIMPTPDPATLL 292

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            +Y+ P  A+ DPD+EIIEGPSR+SL CPISF RIK PVKG SCKHLQCFD+ NY+ IN+R
Sbjct: 293  NYVQPTPASVDPDSEIIEGPSRISLNCPISFKRIKTPVKGQSCKHLQCFDYQNYIDINSR 352

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTES-------P 519
            RP WRCPHC+Q   F DIRIDQ MVKVLKEVGE+++ V + +DGSW++V ES       P
Sbjct: 353  RPSWRCPHCNQHACFTDIRIDQDMVKVLKEVGENVTDVILSSDGSWKAVLESDDPTGNPP 412

Query: 520  EN-----------PEKPKDAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAG 666
             N           P+    A SS     + ++DD MD+                   P+ 
Sbjct: 413  VNKPDISMDETMLPDSNGIASSSADILDLTEIDDAMDVVATGEIEDHKSLQTNCQNQPS- 471

Query: 667  ESSAVNKNAAPHMEDGFWTEYYSMGL-PGT---RTNNSRSDVSEVVQRNQQMFNSITLGQ 834
             S+    N A HM+D FW+ +Y   L PGT    +N     VSE V  +  + + +T   
Sbjct: 472  TSNPSEVNQASHMDDAFWSAFYFPRLEPGTSRPSSNMQIDGVSEPVPTSSMLSSVLTDAL 531

Query: 835  TQS---QIPVSXXXXXXXXXXXXXXXXLYTRY-------QNWVTRTPNAVQALPAQSSAT 984
             Q+   Q   S                +   Y       ++ V+R+P AVQALPAQ  + 
Sbjct: 532  IQASAMQGGDSSPNNLQLQQYQFVNSVIANEYGRMPSIVRHAVSRSPIAVQALPAQMPSP 591

Query: 985  VAN-------GD--------------------------RQQHLSRPQLNQHQVSRMMSS- 1062
            V         GD                           QQ LSR   N  Q S++  S 
Sbjct: 592  VHQQRPRGTIGDVILNVPSATPQATSVSTNAGSSNVEKPQQQLSRSNSNMVQASQVSPST 651

Query: 1063 -QNQGQRDH-PVPVLPAQQHI--SNGRHIPSAH----------FNSYQQHSINQRPTSYP 1200
              N+ Q +H   P+ P QQ +   N  H P+ +           N  +Q   NQ+ T Y 
Sbjct: 652  LPNKQQLEHSSAPIRPTQQFVGHKNPIHTPTPYRASSGLTAESLNRIRQGMANQQ-TPYT 710

Query: 1201 VRSPAHLSPQYRPVGPMTSSQNPQYSM-----------ADVHRATSQATTHTSSI--PTS 1341
                  LS   R   P  S  N Q S+           +  H   + A   T+ I  P  
Sbjct: 711  ANQSPGLS---RSPAPSLSRNNAQGSLQSGVGQARGVASGQHLQPTLAAQRTAQIARPVQ 767

Query: 1342 IVTPGP-------SVTVASTGEQRGGS-----VDGPGEENWRPTGRMRGSLSGRPYSETL 1485
            +    P       S T +  G+QRG +     VD P +++WRPTGRMRGSLSGR Y+E L
Sbjct: 768  LSRAAPPLSANADSPTPSLIGDQRGSTPGTVPVDLPTDQDWRPTGRMRGSLSGRAYNEAL 827

Query: 1486 NQNIIXXXXXXXXXXXXXXXXTPRPFIPPH----LQALMAN 1596
             Q II                 PRP IPP+    LQALMAN
Sbjct: 828  EQYII---------RPTQQAQAPRPSIPPNISPQLQALMAN 859


>ref|XP_019151669.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X2 [Ipomoea nil]
          Length = 893

 Score =  322 bits (826), Expect = 1e-96
 Identities = 240/640 (37%), Positives = 309/640 (48%), Gaps = 108/640 (16%)
 Frame = +1

Query: 1    GVEKRTCVQKDTGPQMPTPVTNMLKYGSNLLQVVGQFTGRYIVAIAFMSVISNPTFPTIP 180
            GVEKRT V  DTGPQ+PT V++ LKYG+NLLQ VG+F G YIVA+AFMS++  P   T+ 
Sbjct: 233  GVEKRTNVLMDTGPQIPTIVSHTLKYGTNLLQAVGEFNGNYIVAVAFMSIMPTPDPATLL 292

Query: 181  DYIPPVAATPDPDNEIIEGPSRVSLKCPISFSRIKIPVKGHSCKHLQCFDFNNYVGINAR 360
            +Y+ P  A+ DPD+EIIEGPSR+SL CPISF RIK PVKG SCKHLQCFD+ NY+ IN+R
Sbjct: 293  NYVQPTPASVDPDSEIIEGPSRISLNCPISFKRIKTPVKGQSCKHLQCFDYQNYIDINSR 352

Query: 361  RPLWRCPHCSQSVSFHDIRIDQSMVKVLKEVGEDISYVKIFNDGSWESVTES-------P 519
            RP WRCPHC+Q   F DIRIDQ MVKVLKEVGE+++ V + +DGSW++V ES       P
Sbjct: 353  RPSWRCPHCNQHACFTDIRIDQDMVKVLKEVGENVTDVILSSDGSWKAVLESDDPTGNPP 412

Query: 520  EN-----------PEKPKDAPSSNQETYIQDVDDIMDLTENXXXXXXXXXXXXXXPSPAG 666
             N           P+    A SS     + ++DD MD+                   P+ 
Sbjct: 413  VNKPDISMDETMLPDSNGIASSSADILDLTEIDDAMDVVATGEIEDHKSLQTNCQNQPS- 471

Query: 667  ESSAVNKNAAPHMEDGFWTEYYSMGL-PGT---RTNNSRSDVSEVVQRNQQMFNSITLGQ 834
             S+    N A HM+D FW+ +Y   L PGT    +N     VSE V  +  + + +T   
Sbjct: 472  TSNPSEVNQASHMDDAFWSAFYFPRLEPGTSRPSSNMQIDGVSEPVPTSSMLSSVLTDAL 531

Query: 835  TQS---QIPVSXXXXXXXXXXXXXXXXLYTRY-------QNWVTRTPNAVQALPAQSSAT 984
             Q+   Q   S                +   Y       ++ V+R+P AVQALPAQ  + 
Sbjct: 532  IQASAMQGGDSSPNNLQLQQYQFVNSVIANEYGRMPSIVRHAVSRSPIAVQALPAQMPSP 591

Query: 985  VAN-------GD--------------------------RQQHLSRPQLNQHQVSRMMSS- 1062
            V         GD                           QQ LSR   N  Q S++  S 
Sbjct: 592  VHQQRPRGTIGDVILNVPSATPQATSVSTNAGSSNVEKPQQQLSRSNSNMVQASQVSPST 651

Query: 1063 -QNQGQRDHPVPVLPAQQHI--SNGRHIPSAH----------FNSYQQHSINQRPTSYPV 1203
              N+       P+ P QQ +   N  H P+ +           N  +Q   NQ+ T Y  
Sbjct: 652  LPNKQLEHSSAPIRPTQQFVGHKNPIHTPTPYRASSGLTAESLNRIRQGMANQQ-TPYTA 710

Query: 1204 RSPAHLSPQYRPVGPMTSSQNPQYSM-----------ADVHRATSQATTHTSSI--PTSI 1344
                 LS   R   P  S  N Q S+           +  H   + A   T+ I  P  +
Sbjct: 711  NQSPGLS---RSPAPSLSRNNAQGSLQSGVGQARGVASGQHLQPTLAAQRTAQIARPVQL 767

Query: 1345 VTPGP-------SVTVASTGEQRGGS-----VDGPGEENWRPTGRMRGSLSGRPYSETLN 1488
                P       S T +  G+QRG +     VD P +++WRPTGRMRGSLSGR Y+E L 
Sbjct: 768  SRAAPPLSANADSPTPSLIGDQRGSTPGTVPVDLPTDQDWRPTGRMRGSLSGRAYNEALE 827

Query: 1489 QNIIXXXXXXXXXXXXXXXXTPRPFIPPH----LQALMAN 1596
            Q II                 PRP IPP+    LQALMAN
Sbjct: 828  QYII---------RPTQQAQAPRPSIPPNISPQLQALMAN 858