BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00028919
         (1220 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KVI08015.1| Diphthamide synthesis DHP2, eukaryotic [Cynara ca...   658   0.0  
ref|XP_022026745.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   612   0.0  
gb|PLY67598.1| hypothetical protein LSAT_2X34381 [Lactuca sativa]     587   0.0  
ref|XP_023741951.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   587   0.0  
ref|XP_023741950.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   587   0.0  
ref|XP_002273627.2| PREDICTED: diphthamide biosynthesis protein ...   545   0.0  
ref|XP_017221407.1| PREDICTED: diphthamide biosynthesis protein ...   536   0.0  
ref|XP_002530564.1| PREDICTED: diphthamide biosynthesis protein ...   526   0.0  
dbj|GAU21097.1| hypothetical protein TSUD_10140 [Trifolium subte...   515   e-178
emb|CDP21132.1| unnamed protein product [Coffea canephora]            514   e-178
ref|XP_004498540.1| PREDICTED: diphthamide biosynthesis protein ...   514   e-178
ref|XP_015868559.1| PREDICTED: diphthamide biosynthesis protein ...   510   e-176
gb|POE97378.1| diphthamide biosynthesis protein 2 [Quercus suber]     508   e-175
ref|XP_019462175.1| PREDICTED: diphthamide biosynthesis protein ...   508   e-175
ref|XP_021675877.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   508   e-175
ref|XP_021675876.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   508   e-175
ref|XP_023923011.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   506   e-175
dbj|GAV84622.1| Diphthamide_syn domain-containing protein [Cepha...   506   e-174
ref|XP_012065078.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   506   e-174
gb|KDO52391.1| hypothetical protein CISIN_1g0110281mg, partial [...   502   e-174

>gb|KVI08015.1| Diphthamide synthesis DHP2, eukaryotic [Cynara cardunculus var.
            scolymus]
          Length = 506

 Score =  658 bits (1697), Expect = 0.0
 Identities = 331/411 (80%), Positives = 362/411 (88%), Gaps = 11/411 (2%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSCEGYNDVKDIG 1022
            +FESKYEV +VATFI++HNFKRVALQFPDELLKDST  V ALR+EL+S  EG NDVK+IG
Sbjct: 2    DFESKYEVSRVATFIRSHNFKRVALQFPDELLKDSTGVVGALRRELRSRWEGENDVKEIG 61

Query: 1021 LYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCAKH 842
            LYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINAS CAKH
Sbjct: 62   LYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASYCAKH 121

Query: 841  LCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMIPT 662
            LC YA S++KP++VLFGLEYGHALQDVIEAS VE T + G TS+ +LHFA+V+ Q+MIP 
Sbjct: 122  LCSYALSSSKPVVVLFGLEYGHALQDVIEASMVEKTSLCGHTSRFELHFADVISQAMIPK 181

Query: 661  NSSETN-----CSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAF 497
             SSETN      S  T +G CG DAV + YRIGGL+W+LEEG +MEDYSLFWIGPDNSAF
Sbjct: 182  KSSETNGQSGLLSSSTGNGGCG-DAVGKTYRIGGLLWSLEEGCSMEDYSLFWIGPDNSAF 240

Query: 496  ANAVLTFNGCDI------VRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVG 335
            AN VLTFNGC+I      +RYDAT NQLL DTSQQRRILKRRYYLVEKAKDA IVGILVG
Sbjct: 241  ANVVLTFNGCEIGTYCIDIRYDATENQLLADTSQQRRILKRRYYLVEKAKDAGIVGILVG 300

Query: 334  TLGVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDS 155
            TLGVAGYL++IHQIKEMITRAGKKAY LVMGKPNP+KLANFPEC+VFIYISCAQTALLDS
Sbjct: 301  TLGVAGYLSVIHQIKEMITRAGKKAYTLVMGKPNPAKLANFPECDVFIYISCAQTALLDS 360

Query: 154  KDFLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            K+FLAPVITPFEAILAF RGSEWTGKYVM+FQDL AST VG EI +KEARF
Sbjct: 361  KEFLAPVITPFEAILAFTRGSEWTGKYVMDFQDLAASTPVGVEIPEKEARF 411


>ref|XP_022026745.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Helianthus
            annuus]
 gb|OTG35720.1| putative diphthamide synthesis DPH2 family protein [Helianthus
            annuus]
          Length = 481

 Score =  612 bits (1579), Expect = 0.0
 Identities = 312/402 (77%), Positives = 344/402 (85%), Gaps = 2/402 (0%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSCEGYNDVKDIG 1022
            EFESKYEV +V TFI+TH FKRVALQFPDELLK+S   V ALRKELQS  E  N+   I 
Sbjct: 2    EFESKYEVLRVVTFIRTHGFKRVALQFPDELLKESIGVVWALRKELQSCGESGNN---IA 58

Query: 1021 LYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCAKH 842
            LYVMADTTYGSCCVDEVGA HINADCVIHYGHTCLSPTS LPAFFVFGKAPINASNCAKH
Sbjct: 59   LYVMADTTYGSCCVDEVGALHINADCVIHYGHTCLSPTSKLPAFFVFGKAPINASNCAKH 118

Query: 841  LCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMIPT 662
            LC+YA S+ KP++VLFGLEYGHA+ D+IEAS +ETTH        +LHFA+VM QSM PT
Sbjct: 119  LCNYALSSKKPVLVLFGLEYGHAIHDIIEASTIETTHD-------KLHFADVMSQSMKPT 171

Query: 661  NSSETNCSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAFANAVL 482
             +S+ N       GDC DDA ++ Y IGGL W+LE+G +MEDYSLFWIGPDNSAFAN VL
Sbjct: 172  KTSDVN-------GDC-DDASQKTYSIGGLRWSLEQGLSMEDYSLFWIGPDNSAFANVVL 223

Query: 481  TFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVAGYLNMI 302
            TFN C+IVRYDAT N LL+DTSQQRRILKRRYYLVEKAKDA IVGILVGTLGVAGYL+MI
Sbjct: 224  TFNDCEIVRYDATENVLLSDTSQQRRILKRRYYLVEKAKDAGIVGILVGTLGVAGYLSMI 283

Query: 301  HQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVITPF 122
            HQIKEM+T+AGKKAY  VMGKPNPSKLANFPEC+VFIYISC+QTALLDSK+FLAPVITPF
Sbjct: 284  HQIKEMVTKAGKKAYTFVMGKPNPSKLANFPECDVFIYISCSQTALLDSKEFLAPVITPF 343

Query: 121  EAILAFNRGSEWTGKYVMEFQDLVASTTVGTEIT--KKEARF 2
            EAILAFNRGSEWTGKYVM+FQDLVAST VG E +  ++EARF
Sbjct: 344  EAILAFNRGSEWTGKYVMQFQDLVASTPVGVEKSGEEEEARF 385


>gb|PLY67598.1| hypothetical protein LSAT_2X34381 [Lactuca sativa]
          Length = 483

 Score =  587 bits (1512), Expect = 0.0
 Identities = 299/402 (74%), Positives = 331/402 (82%), Gaps = 2/402 (0%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSC--EGYNDVKD 1028
            +FESKYEV +VATFI+++ FKRVALQFPDELLKDST  V ALR  L   C     N+VKD
Sbjct: 2    DFESKYEVSRVATFIRSNKFKRVALQFPDELLKDSTRVVGALRNALFGLCCKGDDNEVKD 61

Query: 1027 IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCA 848
            IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPT+ LPAFFVFGKAPINASNCA
Sbjct: 62   IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTTKLPAFFVFGKAPINASNCA 121

Query: 847  KHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMI 668
            KHLC YA STTKP+MVLFGLEY HAL+D+I  ++         +++ +LHFA+VM Q+MI
Sbjct: 122  KHLCGYALSTTKPVMVLFGLEYEHALKDIIIEASA--------SARCKLHFADVMSQAMI 173

Query: 667  PTNSSETNCSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAFANA 488
            P+ S        T   +   DA    YRIGGL W+L EG  M+DYSL +IGPDNSAFAN 
Sbjct: 174  PSPSP-------TKGSEMNGDAATTTYRIGGLFWSLNEGHTMDDYSLCFIGPDNSAFANL 226

Query: 487  VLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVAGYLN 308
            VLTFN C+IVRYDAT N++LTDTSQQRRILKRRYYLVEKAKDA IVGILVGTLGVAGYL+
Sbjct: 227  VLTFNACEIVRYDATENEMLTDTSQQRRILKRRYYLVEKAKDAEIVGILVGTLGVAGYLS 286

Query: 307  MIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVIT 128
            MIHQIKEMITRAGKKAY LVMG+PNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVIT
Sbjct: 287  MIHQIKEMITRAGKKAYTLVMGRPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVIT 346

Query: 127  PFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            PFEAILAFNRGSEWTGKY+MEFQ+L         + +KEARF
Sbjct: 347  PFEAILAFNRGSEWTGKYIMEFQELAVVGMDMMPMPRKEARF 388


>ref|XP_023741951.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform X2
            [Lactuca sativa]
          Length = 485

 Score =  587 bits (1512), Expect = 0.0
 Identities = 299/402 (74%), Positives = 331/402 (82%), Gaps = 2/402 (0%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSC--EGYNDVKD 1028
            +FESKYEV +VATFI+++ FKRVALQFPDELLKDST  V ALR  L   C     N+VKD
Sbjct: 2    DFESKYEVSRVATFIRSNKFKRVALQFPDELLKDSTRVVGALRNALFGLCCKGDDNEVKD 61

Query: 1027 IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCA 848
            IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPT+ LPAFFVFGKAPINASNCA
Sbjct: 62   IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTTKLPAFFVFGKAPINASNCA 121

Query: 847  KHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMI 668
            KHLC YA STTKP+MVLFGLEY HAL+D+I  ++         +++ +LHFA+VM Q+MI
Sbjct: 122  KHLCGYALSTTKPVMVLFGLEYEHALKDIIIEASA--------SARCKLHFADVMSQAMI 173

Query: 667  PTNSSETNCSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAFANA 488
            P+ S        T   +   DA    YRIGGL W+L EG  M+DYSL +IGPDNSAFAN 
Sbjct: 174  PSPSP-------TKGSEMNGDAATTTYRIGGLFWSLNEGHTMDDYSLCFIGPDNSAFANL 226

Query: 487  VLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVAGYLN 308
            VLTFN C+IVRYDAT N++LTDTSQQRRILKRRYYLVEKAKDA IVGILVGTLGVAGYL+
Sbjct: 227  VLTFNACEIVRYDATENEMLTDTSQQRRILKRRYYLVEKAKDAEIVGILVGTLGVAGYLS 286

Query: 307  MIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVIT 128
            MIHQIKEMITRAGKKAY LVMG+PNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVIT
Sbjct: 287  MIHQIKEMITRAGKKAYTLVMGRPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVIT 346

Query: 127  PFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            PFEAILAFNRGSEWTGKY+MEFQ+L         + +KEARF
Sbjct: 347  PFEAILAFNRGSEWTGKYIMEFQELAVVGMDMMPMPRKEARF 388


>ref|XP_023741950.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform X1
            [Lactuca sativa]
          Length = 486

 Score =  587 bits (1512), Expect = 0.0
 Identities = 299/402 (74%), Positives = 331/402 (82%), Gaps = 2/402 (0%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSC--EGYNDVKD 1028
            +FESKYEV +VATFI+++ FKRVALQFPDELLKDST  V ALR  L   C     N+VKD
Sbjct: 2    DFESKYEVSRVATFIRSNKFKRVALQFPDELLKDSTRVVGALRNALFGLCCKGDDNEVKD 61

Query: 1027 IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCA 848
            IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPT+ LPAFFVFGKAPINASNCA
Sbjct: 62   IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTTKLPAFFVFGKAPINASNCA 121

Query: 847  KHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMI 668
            KHLC YA STTKP+MVLFGLEY HAL+D+I  ++         +++ +LHFA+VM Q+MI
Sbjct: 122  KHLCGYALSTTKPVMVLFGLEYEHALKDIIIEASA--------SARCKLHFADVMSQAMI 173

Query: 667  PTNSSETNCSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAFANA 488
            P+ S        T   +   DA    YRIGGL W+L EG  M+DYSL +IGPDNSAFAN 
Sbjct: 174  PSPSP-------TKGSEMNGDAATTTYRIGGLFWSLNEGHTMDDYSLCFIGPDNSAFANL 226

Query: 487  VLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVAGYLN 308
            VLTFN C+IVRYDAT N++LTDTSQQRRILKRRYYLVEKAKDA IVGILVGTLGVAGYL+
Sbjct: 227  VLTFNACEIVRYDATENEMLTDTSQQRRILKRRYYLVEKAKDAEIVGILVGTLGVAGYLS 286

Query: 307  MIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVIT 128
            MIHQIKEMITRAGKKAY LVMG+PNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVIT
Sbjct: 287  MIHQIKEMITRAGKKAYTLVMGRPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPVIT 346

Query: 127  PFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            PFEAILAFNRGSEWTGKY+MEFQ+L         + +KEARF
Sbjct: 347  PFEAILAFNRGSEWTGKYIMEFQELAVVGMDMMPMPRKEARF 388


>ref|XP_002273627.2| PREDICTED: diphthamide biosynthesis protein 2 isoform X1 [Vitis
            vinifera]
 emb|CBI24661.3| unnamed protein product, partial [Vitis vinifera]
          Length = 506

 Score =  545 bits (1403), Expect = 0.0
 Identities = 273/406 (67%), Positives = 320/406 (78%), Gaps = 6/406 (1%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSCE-GYNDVKDI 1025
            E ES YE+   A FI++ NF RVALQFPDELLKD+T  V ALR  L+S  E G  D K++
Sbjct: 2    ELESNYEIALTADFIRSRNFTRVALQFPDELLKDATRVVKALRDRLESPSESGSGDGKEV 61

Query: 1024 GLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCAK 845
            GL+VMADTTYGSCCVDEVGASHINADCV+HYGHTCLSPTSNLPAFFVFGKAPI+ SNCA+
Sbjct: 62   GLFVMADTTYGSCCVDEVGASHINADCVVHYGHTCLSPTSNLPAFFVFGKAPISISNCAE 121

Query: 844  HLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMIP 665
            +L  YA +  K IMVLFGLEY HA++ + EA  +E++ +SG TS ++ HFAEVM   M P
Sbjct: 122  NLSTYALTQGKHIMVLFGLEYSHAIEMIKEALMLESSKLSGSTSNLEFHFAEVMCSVMNP 181

Query: 664  TNSSETNCSRCTADGDCGDD-----AVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSA 500
            +    T+     + G    +     A   +Y IGGL WNL EG  MEDY L WIG DNSA
Sbjct: 182  SKDCTTSNGHLGSVGSFTANEGFGKATGTRYSIGGLAWNLPEGHRMEDYLLVWIGADNSA 241

Query: 499  FANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVA 320
            FAN VLTFNGCDIVRYDA  N+L+TD SQQRRILKRRYYLVEKAKD+ I+G+LVGTLGVA
Sbjct: 242  FANVVLTFNGCDIVRYDAVDNRLVTDLSQQRRILKRRYYLVEKAKDSNIIGLLVGTLGVA 301

Query: 319  GYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLA 140
            GYL+MI Q+KE+I  AGKKAY LVMG+PNP+KLANFPEC+VFIY+SCAQTALLDSK+FLA
Sbjct: 302  GYLHMIRQMKELIAAAGKKAYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLDSKEFLA 361

Query: 139  PVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            PVITPFEA+LAFNRGS+WTG YVMEF+DL++S+ V      +EARF
Sbjct: 362  PVITPFEAMLAFNRGSQWTGAYVMEFRDLISSSPVEVRNQSEEARF 407


>ref|XP_017221407.1| PREDICTED: diphthamide biosynthesis protein 2 [Daucus carota subsp.
            sativus]
 gb|KZM84270.1| hypothetical protein DCAR_028436 [Daucus carota subsp. sativus]
          Length = 515

 Score =  536 bits (1382), Expect = 0.0
 Identities = 268/410 (65%), Positives = 324/410 (79%), Gaps = 10/410 (2%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKEL----QSSCEGYNDV 1034
            + E  YE+  VA FI T +F+RVALQFPDELLK+ST  V ALR++L    +S+ E   DV
Sbjct: 2    DMEGFYEIEAVARFILTRDFRRVALQFPDELLKESTRVVRALREKLHVLKKSTGERDRDV 61

Query: 1033 KDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASN 854
            +DIGLYVM DTTYGSCCVDEV ASHI+ADCVIHYGHTCLSPTSNLPAFFVFGKA I+ASN
Sbjct: 62   RDIGLYVMGDTTYGSCCVDEVAASHIHADCVIHYGHTCLSPTSNLPAFFVFGKASISASN 121

Query: 853  CAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQS 674
             A+ LC YAS++ KPI+VLFGLEY +A++++ E    E++ + G T+ + +H+A+V+   
Sbjct: 122  SAESLCKYASASGKPILVLFGLEYAYAMEEIKEIIAHESSKLCGSTTILDIHYADVICSI 181

Query: 673  MIPTNSSETN------CSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGP 512
            M PT  SET+       S  T+DG C        Y IGGL W++ EG  +EDY+LFWIG 
Sbjct: 182  MNPTGKSETSDEHLESFSISTSDG-CSGKTTATSYNIGGLGWSVPEGSRIEDYTLFWIGS 240

Query: 511  DNSAFANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGT 332
            DNSAFA+ VLTFNGC+IVRYDA  N ++TD +QQRRILKRRYYLVEKAKDA+IVGILVGT
Sbjct: 241  DNSAFASVVLTFNGCEIVRYDAKENHIITDFTQQRRILKRRYYLVEKAKDASIVGILVGT 300

Query: 331  LGVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSK 152
            LGVAGYL MIHQ+KE+IT AGKKAY L MG+PNP+KLANFPEC+VFIY+SCAQTALLDSK
Sbjct: 301  LGVAGYLRMIHQMKELITAAGKKAYTLAMGRPNPAKLANFPECDVFIYVSCAQTALLDSK 360

Query: 151  DFLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            +FLAPVITP+EA+LAF+R ++WTG YVMEFQ L++ST  G     KEARF
Sbjct: 361  EFLAPVITPYEAMLAFSRNTQWTGAYVMEFQQLISSTQTGVRSPSKEARF 410


>ref|XP_002530564.1| PREDICTED: diphthamide biosynthesis protein 2 [Ricinus communis]
 gb|EEF31794.1| diphteria toxin resistance protein 2, dph2, putative [Ricinus
            communis]
          Length = 499

 Score =  526 bits (1354), Expect = 0.0
 Identities = 264/405 (65%), Positives = 311/405 (76%), Gaps = 5/405 (1%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSCEGYNDVKDIG 1022
            +FE  YE+     FI + NF RVALQFPDELLKDS   V ALR +L+   E   D K+I 
Sbjct: 2    DFEENYEIDLTTDFIHSRNFTRVALQFPDELLKDSVRVVSALRNKLELLEENGGDRKEIR 61

Query: 1021 LYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCAKH 842
            L+VMADT YGSCCVDEVGASH NADCVIHYGHTCLSPTSNLPAFFVFGKA IN S+CA+ 
Sbjct: 62   LFVMADTAYGSCCVDEVGASHANADCVIHYGHTCLSPTSNLPAFFVFGKASINVSSCAQS 121

Query: 841  LCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMIPT 662
            L DYA S+TKPIMVL+GLEY HA+   I    +    M       +LHFA+VM  ++ P+
Sbjct: 122  LSDYAFSSTKPIMVLYGLEYAHAIPH-IRQELIAAASMQSRPRHSELHFADVMCSAINPS 180

Query: 661  NSSETNCSRCTADGDCG-----DDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAF 497
            N+ + +       GDC       +A   +++IGGL+W+L  GQ MEDY L WIG  NSAF
Sbjct: 181  NNQKKSDEPARLIGDCVATNDVGEAAGCRHKIGGLIWDLSRGQRMEDYLLVWIGSGNSAF 240

Query: 496  ANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVAG 317
            AN VLTFNGC+IVRYDA  ++L+TD SQQRRILKRRYYLVEKAKDA IVGILVGTLGVAG
Sbjct: 241  ANVVLTFNGCEIVRYDAKESRLVTDLSQQRRILKRRYYLVEKAKDANIVGILVGTLGVAG 300

Query: 316  YLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLAP 137
            YL+MIHQ+K++IT AGKKAY LVMG+PNP+KLANFPEC++FIY+SCAQTALLDSK+FLAP
Sbjct: 301  YLDMIHQMKDLITSAGKKAYTLVMGRPNPAKLANFPECDIFIYVSCAQTALLDSKEFLAP 360

Query: 136  VITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            VITPFEA++AFNRGS+WTG YVMEF+DL+ S+ V   I   EARF
Sbjct: 361  VITPFEAMIAFNRGSQWTGAYVMEFRDLIDSSPVEAMIKPAEARF 405


>dbj|GAU21097.1| hypothetical protein TSUD_10140 [Trifolium subterraneum]
          Length = 502

 Score =  515 bits (1326), Expect = e-178
 Identities = 262/410 (63%), Positives = 311/410 (75%), Gaps = 10/410 (2%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSCE----GYNDV 1034
            +FES Y++   + FI+THNF RVALQFPD+LLKDSTE V  LRK LQS  E    G  + 
Sbjct: 2    DFESNYDIAATSDFIRTHNFTRVALQFPDDLLKDSTEVVAVLRKRLQSLRESDITGSAEE 61

Query: 1033 KDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASN 854
             DIGLYVMADT YGSCCVDEVGASHINADCVIHYGHTC SPT++LPAFFVFGK+ I  ++
Sbjct: 62   TDIGLYVMADTAYGSCCVDEVGASHINADCVIHYGHTCFSPTTSLPAFFVFGKSSIGIAD 121

Query: 853  CAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQS 674
            C + +  YA + +KPIMVLFGLEY H++Q +  A    +T+   D  K ++HFA+V   +
Sbjct: 122  CVESMSQYALTNSKPIMVLFGLEYAHSIQQIKNALLESSTYCKSD-HKPEVHFADVPSPN 180

Query: 673  MIPT------NSSETNCSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGP 512
            M P+      N  +     C  +    D A    Y IGGL W L EGQ+MEDYSLFWIG 
Sbjct: 181  MFPSKDIKKLNGIQEEACGC-GNNSSSDGASGTIYNIGGLTWKLPEGQSMEDYSLFWIGQ 239

Query: 511  DNSAFANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGT 332
            DNSAFAN VLTFN C+IVRYDAT NQ++TD SQQRRILKRRYYLVE+AKDA IVGILVGT
Sbjct: 240  DNSAFANVVLTFNACEIVRYDATKNQMVTDLSQQRRILKRRYYLVERAKDANIVGILVGT 299

Query: 331  LGVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSK 152
            LGVAGYL++I+Q+ E+IT AGKKAY LVMGKPNP+KLANFPEC+VF+Y+SCAQTALLDSK
Sbjct: 300  LGVAGYLHIINQMMELITAAGKKAYTLVMGKPNPAKLANFPECDVFLYVSCAQTALLDSK 359

Query: 151  DFLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            ++LAPVITPFEA +AFNRGS+WTG YVM F DL+    V     ++EARF
Sbjct: 360  EYLAPVITPFEATIAFNRGSQWTGAYVMGFGDLINLPQVHAG-NQEEARF 408


>emb|CDP21132.1| unnamed protein product [Coffea canephora]
          Length = 495

 Score =  514 bits (1323), Expect = e-178
 Identities = 259/405 (63%), Positives = 306/405 (75%), Gaps = 5/405 (1%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSCEGYNDVKDIG 1022
            +FE+ YE+ +VA FI  HNF RVALQFPDELLKDS + V ALR  LQ+S E +   K   
Sbjct: 2    DFENYYEITRVAGFIHAHNFSRVALQFPDELLKDSRKVVSALRHLLQNSDESHGTAK--- 58

Query: 1021 LYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCAKH 842
            LYVMADTTYGSCCVDEVGA+H+ ADCVIHYGHTCLSPT+ +PAF VFGKAP+ ASNCA+ 
Sbjct: 59   LYVMADTTYGSCCVDEVGAAHVKADCVIHYGHTCLSPTTTIPAFCVFGKAPLKASNCAES 118

Query: 841  LCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMIPT 662
            L +Y     KPI+VLFGLEY HA+ D+ EA NVE        S  Q+HFA V    + P+
Sbjct: 119  LYNYTVKGGKPILVLFGLEYAHAMPDIKEALNVEAVRFHDSNSGFQIHFANVTNSVITPS 178

Query: 661  NSS--ETNCSRCTADGDCGDDAVKR---KYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAF 497
             S   E        D D  +D+ ++   K+R+GGL W+L EG  MEDY LFWIG DNSAF
Sbjct: 179  TSMKLENRHLMLNGDQDNNEDSSQKTDTKFRLGGLGWSLPEGHRMEDYLLFWIGLDNSAF 238

Query: 496  ANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVAG 317
             N +LTFN C+IVRYDA    L TD SQQ +ILKRRYYLVEKAKDA +VGILVGTLGVAG
Sbjct: 239  TNFILTFNSCEIVRYDAIEGCLKTDVSQQAKILKRRYYLVEKAKDANMVGILVGTLGVAG 298

Query: 316  YLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLAP 137
            YL+MIHQ+ E++TRAGKKAY  +MGKPNP+KLANFPEC+VFIY+SCAQTALLDSK+FLAP
Sbjct: 299  YLDMIHQMTELVTRAGKKAYTFLMGKPNPAKLANFPECDVFIYVSCAQTALLDSKEFLAP 358

Query: 136  VITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            +ITPFEA++AFNRGS+WTG Y MEFQD+VAS     E  + E+RF
Sbjct: 359  IITPFEALIAFNRGSQWTGAYSMEFQDVVASQPAEVE-DEAESRF 402


>ref|XP_004498540.1| PREDICTED: diphthamide biosynthesis protein 2 [Cicer arietinum]
          Length = 501

 Score =  514 bits (1323), Expect = e-178
 Identities = 265/409 (64%), Positives = 308/409 (75%), Gaps = 9/409 (2%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSCE----GYNDV 1034
            +FES Y++   A FI THNF RVALQFPD+LLKDST+ V  LRK LQS  E    G  D 
Sbjct: 2    DFESNYDIAATAHFILTHNFTRVALQFPDDLLKDSTKVVTVLRKRLQSLRECDITGSGDE 61

Query: 1033 KDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASN 854
             +IGLYVMADT YGSCCVDEVGASHINADCVIHYGHTC SPT+ LPAFFVFGK+ I A++
Sbjct: 62   TEIGLYVMADTAYGSCCVDEVGASHINADCVIHYGHTCFSPTTTLPAFFVFGKSSICAAD 121

Query: 853  CAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQS 674
            C + L  YA + +KPIMVLFGLEY H++Q + +     +T    D  K + HFA+V    
Sbjct: 122  CVERLSQYALTNSKPIMVLFGLEYAHSIQQIRKVLLESSTSCRSDF-KSEFHFADVPSAF 180

Query: 673  MIPTNSSETNCSRCTADGDCG-----DDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPD 509
            M P+   +       A  DCG     D A    Y IGGL W L EGQ+MEDYSLFWIG D
Sbjct: 181  MFPSKDIKKLNGLQEAACDCGHKSSSDGASGTVYNIGGLTWKLPEGQSMEDYSLFWIGHD 240

Query: 508  NSAFANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTL 329
            NSAFAN VLTFN C+IVRYDAT NQ++TD SQQRRILKRRYYLVE+AKDA IVGILVGTL
Sbjct: 241  NSAFANIVLTFNACEIVRYDATKNQMVTDLSQQRRILKRRYYLVERAKDANIVGILVGTL 300

Query: 328  GVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKD 149
            GVAGYL++I+Q+ E+IT AGKKAY LVMGKPNP+KLANFPEC+VF+Y+SCAQTALLDSK+
Sbjct: 301  GVAGYLHIINQMMELITGAGKKAYTLVMGKPNPAKLANFPECDVFVYVSCAQTALLDSKE 360

Query: 148  FLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            +LAPVITPFEA +AFNRGS+WTG YVM F DL+    V     ++EARF
Sbjct: 361  YLAPVITPFEATIAFNRGSQWTGAYVMGFGDLINLPQVQVG-NQEEARF 408


>ref|XP_015868559.1| PREDICTED: diphthamide biosynthesis protein 2 [Ziziphus jujuba]
          Length = 504

 Score =  510 bits (1313), Expect = e-176
 Identities = 255/411 (62%), Positives = 304/411 (73%), Gaps = 11/411 (2%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALR------KELQSSCEGYN 1040
            + ES YE+ + A FI    F RVALQFPDELLKDS   V  LR      +E QS  +   
Sbjct: 2    DLESNYEIARTAKFISGGGFTRVALQFPDELLKDSVRVVSVLRDKLRRLRESQSDSDRNG 61

Query: 1039 DVKDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINA 860
            DVKD+ L+VMADT YGSCCVDE GASHINADCV+HYGHTC SPT+ LPAFFVFGKA I+ 
Sbjct: 62   DVKDVSLFVMADTAYGSCCVDEAGASHINADCVVHYGHTCFSPTTTLPAFFVFGKASISV 121

Query: 859  SNCAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMG 680
            SNC K L D+A    KP++VLFGLEY H +Q + +    E       +S +++ FAE M 
Sbjct: 122  SNCVKKLSDHAVRNGKPVLVLFGLEYAHLIQQIRD----EVVETCTSSSGLEIQFAETMS 177

Query: 679  QSMIPTNSSE-----TNCSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIG 515
              M P+ S +     +  +   +D  C       +Y IGGLVW L EG  MEDY LFWIG
Sbjct: 178  SDMKPSESHKISNGSSEPASVFSDDKCIRTVAGNRYSIGGLVWELREGHKMEDYLLFWIG 237

Query: 514  PDNSAFANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVG 335
             DN+AFA+ VLTFNGC+IVRYDA +N ++TD SQQRRILKRRYYL+EKAKDA IVGILVG
Sbjct: 238  SDNAAFASVVLTFNGCEIVRYDARSNCMVTDLSQQRRILKRRYYLIEKAKDANIVGILVG 297

Query: 334  TLGVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDS 155
            TLGVAGYL+MI+Q+KE+IT  GKKAY LVMG+PNP+KLANFPEC++FIY+SCAQTALLDS
Sbjct: 298  TLGVAGYLHMINQMKELITATGKKAYTLVMGRPNPAKLANFPECDIFIYVSCAQTALLDS 357

Query: 154  KDFLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            K++LAPVITPFEA+LAFNRGSEWTG YVMEFQDL+ S+ + +    KEARF
Sbjct: 358  KEYLAPVITPFEAMLAFNRGSEWTGAYVMEFQDLINSSPLVSRNQSKEARF 408


>gb|POE97378.1| diphthamide biosynthesis protein 2 [Quercus suber]
          Length = 508

 Score =  508 bits (1308), Expect = e-175
 Identities = 256/411 (62%), Positives = 316/411 (76%), Gaps = 11/411 (2%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQ-----SSCEGYND 1037
            + ES YE+   A FI + NF RVALQFPDELLKDST  V ALR++L+     S+    ++
Sbjct: 2    DLESNYEIAPTADFIYSRNFSRVALQFPDELLKDSTRVVRALREKLRLLRKCSTENSGDN 61

Query: 1036 VKDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINAS 857
             KD+ L+VMADTT+GSCCVDEVGA H +A+CV+HYGHTCLSPT+ LPAFFVFGKA I+AS
Sbjct: 62   NKDVRLFVMADTTFGSCCVDEVGALHADAECVVHYGHTCLSPTTTLPAFFVFGKASISAS 121

Query: 856  NCAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQ 677
            NC + L +YAS+  K ++VL+GLEY H+++ + EA   E + +S   SK ++HFA+V   
Sbjct: 122  NCVEDLLNYASTNGKRVVVLYGLEYAHSIKHIREALE-EASRLSQSKSKSEIHFADVKCS 180

Query: 676  SMIPTNSSETN------CSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIG 515
             M P++    +       S CT +  CG       Y IGGL W++ +GQ MEDY LFWIG
Sbjct: 181  VMNPSDDHNNSNGLVEPTSDCTDNSTCGTTPAN-SYSIGGLTWSIPDGQRMEDYLLFWIG 239

Query: 514  PDNSAFANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVG 335
             DNSAFAN V+TFNGCDIVRYDAT N L+TD SQ++RILKRRYYLVEKAKDA I+GILVG
Sbjct: 240  SDNSAFANVVMTFNGCDIVRYDATENCLVTDVSQRKRILKRRYYLVEKAKDANIIGILVG 299

Query: 334  TLGVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDS 155
            TLGVAGYL+MIHQ+KE+IT AGKKAY LVMGKPNP+KLANFPEC+VFI++SCAQTALL+S
Sbjct: 300  TLGVAGYLHMIHQMKELITAAGKKAYTLVMGKPNPAKLANFPECDVFIFVSCAQTALLES 359

Query: 154  KDFLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            K++L+PVITPFEA+LAFNRGS+WTG+YVMEF+DL+ S  V       EARF
Sbjct: 360  KEYLSPVITPFEAMLAFNRGSQWTGEYVMEFRDLINSCPVEVR-NGSEARF 409


>ref|XP_019462175.1| PREDICTED: diphthamide biosynthesis protein 2 [Lupinus angustifolius]
          Length = 501

 Score =  508 bits (1307), Expect = e-175
 Identities = 261/410 (63%), Positives = 313/410 (76%), Gaps = 10/410 (2%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSSCE----GYNDV 1034
            + ES Y++   A FI  HNF RVALQFPD+LLKDST  V ALRK LQS  E    G    
Sbjct: 2    DLESNYDIAATAHFIHNHNFTRVALQFPDDLLKDSTRVVFALRKRLQSLRESDVAGNGGE 61

Query: 1033 KDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASN 854
            K+IGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTC SPT+ LPAFFVFGKA I  ++
Sbjct: 62   KEIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCFSPTTTLPAFFVFGKASICVAD 121

Query: 853  CAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQS 674
            CA+ +  YA + +KPIMVLFGLEY H++Q + EA  +E +  S    K ++HFA+V   +
Sbjct: 122  CAESISKYALTNSKPIMVLFGLEYAHSIQQIREAL-LEPSLSSRYDLKSEVHFADVPTSA 180

Query: 673  MIPTNSSETNCSRCTADGDCGDDAVK-----RKYRIGGLVWNLEEGQNMEDYSLFWIGPD 509
            + P+            D DC ++A        +  IGGL W L EGQ+MEDYSLFWIG D
Sbjct: 181  IFPSKDINKLDGPQQLDSDCSNNASADGTSGTRCCIGGLTWKLPEGQSMEDYSLFWIGHD 240

Query: 508  NSAFANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTL 329
            NSAFAN VLTFN C+IVRYDA  NQ++TD SQQRRILKRRYYLVE+AKDA IVGILVGTL
Sbjct: 241  NSAFANIVLTFNACEIVRYDAPENQMMTDLSQQRRILKRRYYLVERAKDANIVGILVGTL 300

Query: 328  GVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKD 149
            GVAGYL++I+Q+ E+IT AGKKAY LVMGKPNP+KLANFPEC++FIY+SCAQTALLDSK+
Sbjct: 301  GVAGYLHIINQMMELITGAGKKAYTLVMGKPNPAKLANFPECDIFIYVSCAQTALLDSKE 360

Query: 148  FLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITK-KEARF 2
            +L+PVITPFEA +AF+RGS+WTG Y M+F DL+ +T +  E+ K +EARF
Sbjct: 361  YLSPVITPFEATIAFSRGSQWTGAYAMDFGDLINNTPL--EVGKQEEARF 408


>ref|XP_021675877.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform X2
            [Hevea brasiliensis]
          Length = 506

 Score =  508 bits (1307), Expect = e-175
 Identities = 260/404 (64%), Positives = 306/404 (75%), Gaps = 9/404 (2%)
 Frame = -2

Query: 1186 YEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQ----SSCEGYNDVKDIGL 1019
            YE+ + A FI   NF +VALQFPDELLKDS   V ALR +LQ    S  E   D K + L
Sbjct: 7    YEIDRTADFIHNKNFTKVALQFPDELLKDSVRVVSALRDKLQLLRESVTEQNGDSKQVRL 66

Query: 1018 YVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCAKHL 839
            +VMADTTYGSCCVDEVGASH NADCVIHYGHTCLSPTS LPAFFVFGKA I+ SNCAK L
Sbjct: 67   FVMADTTYGSCCVDEVGASHANADCVIHYGHTCLSPTSTLPAFFVFGKASISISNCAKSL 126

Query: 838  CDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMIPTN 659
             DYAS+ +KPI+VL+GLEY HA+  V E     ++  SG   +  +HFA+V+   + P++
Sbjct: 127  SDYASTNSKPIVVLYGLEYVHAIPHVREELVALSSMQSGPKHQ-DIHFADVVCSVINPSD 185

Query: 658  SSETNCSRCTADGDCG-----DDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAFA 494
              +         GD       + A    YRIGGL+W L  G  MEDY L W+G  NSAFA
Sbjct: 186  DQKNPVGLPRPVGDYSASVEVEAAAGSSYRIGGLIWELPNGHRMEDYLLVWVGSSNSAFA 245

Query: 493  NAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVAGY 314
            N VLTFNGC+IVRYDA  N+L+TD SQQRRILKRRYYLVEKAKDA IVGILVGTLGVAGY
Sbjct: 246  NVVLTFNGCEIVRYDALENRLVTDLSQQRRILKRRYYLVEKAKDANIVGILVGTLGVAGY 305

Query: 313  LNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPV 134
            L+MIHQ+KE+IT AGKKAY LVMG+PNP+KLANFPEC+VFIY+SCAQTALLDSK+FLAPV
Sbjct: 306  LDMIHQMKELITSAGKKAYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLDSKEFLAPV 365

Query: 133  ITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            ITPFEA++AF RG +W+G+YVMEF+DL+ S+ V  + T +EARF
Sbjct: 366  ITPFEAMVAFGRGRQWSGEYVMEFRDLINSSPVEAKDTSEEARF 409


>ref|XP_021675876.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform X1
            [Hevea brasiliensis]
          Length = 519

 Score =  508 bits (1307), Expect = e-175
 Identities = 260/404 (64%), Positives = 306/404 (75%), Gaps = 9/404 (2%)
 Frame = -2

Query: 1186 YEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQ----SSCEGYNDVKDIGL 1019
            YE+ + A FI   NF +VALQFPDELLKDS   V ALR +LQ    S  E   D K + L
Sbjct: 7    YEIDRTADFIHNKNFTKVALQFPDELLKDSVRVVSALRDKLQLLRESVTEQNGDSKQVRL 66

Query: 1018 YVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASNCAKHL 839
            +VMADTTYGSCCVDEVGASH NADCVIHYGHTCLSPTS LPAFFVFGKA I+ SNCAK L
Sbjct: 67   FVMADTTYGSCCVDEVGASHANADCVIHYGHTCLSPTSTLPAFFVFGKASISISNCAKSL 126

Query: 838  CDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQSMIPTN 659
             DYAS+ +KPI+VL+GLEY HA+  V E     ++  SG   +  +HFA+V+   + P++
Sbjct: 127  SDYASTNSKPIVVLYGLEYVHAIPHVREELVALSSMQSGPKHQ-DIHFADVVCSVINPSD 185

Query: 658  SSETNCSRCTADGDCG-----DDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAFA 494
              +         GD       + A    YRIGGL+W L  G  MEDY L W+G  NSAFA
Sbjct: 186  DQKNPVGLPRPVGDYSASVEVEAAAGSSYRIGGLIWELPNGHRMEDYLLVWVGSSNSAFA 245

Query: 493  NAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVAGY 314
            N VLTFNGC+IVRYDA  N+L+TD SQQRRILKRRYYLVEKAKDA IVGILVGTLGVAGY
Sbjct: 246  NVVLTFNGCEIVRYDALENRLVTDLSQQRRILKRRYYLVEKAKDANIVGILVGTLGVAGY 305

Query: 313  LNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPV 134
            L+MIHQ+KE+IT AGKKAY LVMG+PNP+KLANFPEC+VFIY+SCAQTALLDSK+FLAPV
Sbjct: 306  LDMIHQMKELITSAGKKAYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLDSKEFLAPV 365

Query: 133  ITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            ITPFEA++AF RG +W+G+YVMEF+DL+ S+ V  + T +EARF
Sbjct: 366  ITPFEAMVAFGRGRQWSGEYVMEFRDLINSSPVEAKDTSEEARF 409


>ref|XP_023923011.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2-like [Quercus
            suber]
          Length = 508

 Score =  506 bits (1304), Expect = e-175
 Identities = 255/411 (62%), Positives = 316/411 (76%), Gaps = 11/411 (2%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQ-----SSCEGYND 1037
            + ES YE+   A FI + NF RVALQFPD+LLKDST  V ALR++L+     S+    ++
Sbjct: 2    DLESNYEIAPTADFIYSRNFTRVALQFPDDLLKDSTRVVRALREKLRLLRKCSTENSGDN 61

Query: 1036 VKDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINAS 857
             KD+ L+VMADTT+GSCCVDEVGA H +A+CV+HYGHTCLSPT+ LPAFFVFGKA I+AS
Sbjct: 62   NKDVRLFVMADTTFGSCCVDEVGALHADAECVVHYGHTCLSPTTTLPAFFVFGKASISAS 121

Query: 856  NCAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQ 677
            NC + L +YAS+  K ++VL+GLEY H+++ + EA   E + +S   SK ++HFA+V   
Sbjct: 122  NCVEDLLNYASTNGKRVVVLYGLEYAHSIKHIREALE-EASRLSQSKSKSEIHFADVKCS 180

Query: 676  SMIPTNSSETN------CSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIG 515
             M P++    +       S CT +  CG       Y IGGL W++ +GQ MEDY LFWIG
Sbjct: 181  VMNPSDDHNNSNGLVEPTSDCTDNSTCGTTPAN-SYSIGGLTWSIPDGQRMEDYLLFWIG 239

Query: 514  PDNSAFANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVG 335
             DNSAFAN V+TFNGCDIVRYDAT N L+TD SQ++RILKRRYYLVEKAKDA I+GILVG
Sbjct: 240  SDNSAFANVVMTFNGCDIVRYDATENCLVTDVSQRKRILKRRYYLVEKAKDANIIGILVG 299

Query: 334  TLGVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDS 155
            TLGVAGYL+MIHQ+KE+IT AGKKAY LVMGKPNP+KLANFPEC+VFI++SCAQTALL+S
Sbjct: 300  TLGVAGYLHMIHQMKELITAAGKKAYTLVMGKPNPAKLANFPECDVFIFVSCAQTALLES 359

Query: 154  KDFLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            K++L+PVITPFEA+LAFNRGS+WTG+YVMEF+DL+ S  V       EARF
Sbjct: 360  KEYLSPVITPFEAMLAFNRGSQWTGEYVMEFRDLINSCPVEVR-NGSEARF 409


>dbj|GAV84622.1| Diphthamide_syn domain-containing protein [Cephalotus follicularis]
          Length = 507

 Score =  506 bits (1303), Expect = e-174
 Identities = 257/411 (62%), Positives = 306/411 (74%), Gaps = 11/411 (2%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKEL----QSSCEGYNDV 1034
            E ES YE+   A FI + NFKRVALQFPDELLKDST  V  LR  L    +S  EG  D 
Sbjct: 2    EIESNYEIALTADFIHSRNFKRVALQFPDELLKDSTRVVSGLRDRLKSLRESDAEGNADD 61

Query: 1033 KDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASN 854
            K IGL++MADTTYGSCCVDEVGASHI+ADCVIHYGHTCLSPTS LPAF VFGKA I+ S+
Sbjct: 62   KGIGLFIMADTTYGSCCVDEVGASHIDADCVIHYGHTCLSPTSTLPAFCVFGKASISVSD 121

Query: 853  CAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQS 674
            C ++L  YA +  KPI+VL+GLEY HA+  + +A   E +      SK++LHF +VM   
Sbjct: 122  CVENLSKYALTNGKPILVLYGLEYAHAMPSIRQALT-EASVSCVSASKLELHFTDVMCSV 180

Query: 673  MIPTNSSETNCS-------RCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIG 515
            + P+  +  +         + + D  C        + IGGL+W++ +G  MEDY LFWIG
Sbjct: 181  INPSEDNRNSIGVLGLSSGQISTDSSCA--VADSSFSIGGLIWDIPKGHRMEDYLLFWIG 238

Query: 514  PDNSAFANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVG 335
             D SAFAN VLTFNGC+IVRYDA  N L+TD SQ+RRILKRRYYLVEKAKDA I+GILVG
Sbjct: 239  SDGSAFANIVLTFNGCEIVRYDAKENHLVTDFSQKRRILKRRYYLVEKAKDANIIGILVG 298

Query: 334  TLGVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDS 155
            TLGVAGYL+MIHQ+KE+I  AGKKAY LVMGKPNP+KLANFPEC+VFI +SC QTALLDS
Sbjct: 299  TLGVAGYLHMIHQLKELIIGAGKKAYTLVMGKPNPAKLANFPECDVFICVSCPQTALLDS 358

Query: 154  KDFLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            K+FLAPVITPFEA+LAFNRGS+WTG YVMEF+DL+ S+ +      +EARF
Sbjct: 359  KEFLAPVITPFEAMLAFNRGSQWTGAYVMEFRDLIDSSPLEVRKQSEEARF 409


>ref|XP_012065078.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform X1
            [Jatropha curcas]
 gb|KDP44261.1| hypothetical protein JCGZ_05728 [Jatropha curcas]
          Length = 508

 Score =  506 bits (1303), Expect = e-174
 Identities = 261/412 (63%), Positives = 311/412 (75%), Gaps = 9/412 (2%)
 Frame = -2

Query: 1210 MVDEFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQSS----CEGY 1043
            M  +FE  YE+ + A FI + NF RVALQFPDELLKDS   V ALR +LQS      E  
Sbjct: 1    MAMDFEENYEINRTADFIHSRNFTRVALQFPDELLKDSVRVVSALRDKLQSLRASIAEKI 60

Query: 1042 NDVKDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPIN 863
             + K+  L+VMADTTYGSCCVDEVGASHI+ADCVIHYGHTCLSPTS LPAFFVFGKA I+
Sbjct: 61   GENKEARLFVMADTTYGSCCVDEVGASHIDADCVIHYGHTCLSPTSTLPAFFVFGKASIS 120

Query: 862  ASNCAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVM 683
              +CA+ L DYAS+ +KPI+V++GLEY HA+  + E     ++  SG      L FA+VM
Sbjct: 121  IHSCAQSLSDYASTNSKPIVVIYGLEYAHAIPHIKEKFMAASSIESGPKHSQSL-FADVM 179

Query: 682  GQSMIPTNSSETNCSRCTADGDCG-----DDAVKRKYRIGGLVWNLEEGQNMEDYSLFWI 518
               + P +S + +       GD       D AV  K++IGGL+W L  G+ MEDY L WI
Sbjct: 180  CSVINPFDSHKNSDGLPKLLGDYNASNDVDAAVGSKHKIGGLIWELPNGRRMEDYLLVWI 239

Query: 517  GPDNSAFANAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILV 338
            G D+SAFAN VLTFNGC+IVRYDA  N+L+TD SQQRRILKRRYYLVEKAKDA IVGILV
Sbjct: 240  GSDSSAFANVVLTFNGCEIVRYDAKENRLVTDLSQQRRILKRRYYLVEKAKDANIVGILV 299

Query: 337  GTLGVAGYLNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLD 158
            GTLGVAGYL+MIHQ+K++IT AGKK Y LVMG+PNP+KLANFPEC+VFIY+SCAQTALLD
Sbjct: 300  GTLGVAGYLDMIHQMKDLITNAGKKTYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLD 359

Query: 157  SKDFLAPVITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            SK+FLAPVITPFEA++AFNRGS WTG+YVMEF+DL+ S+        +EARF
Sbjct: 360  SKEFLAPVITPFEAMVAFNRGSLWTGEYVMEFRDLINSSPAEARNNSEEARF 411


>gb|KDO52391.1| hypothetical protein CISIN_1g0110281mg, partial [Citrus sinensis]
          Length = 409

 Score =  502 bits (1292), Expect = e-174
 Identities = 258/404 (63%), Positives = 312/404 (77%), Gaps = 4/404 (0%)
 Frame = -2

Query: 1201 EFESKYEVPQVATFIQTHNFKRVALQFPDELLKDSTEAVLALRKELQS--SCEGYNDV-- 1034
            +FE  YE+   A FI +  F RVALQFPD LLKDS   V ALR +L S  S +G   V  
Sbjct: 2    DFELNYEIASTADFIYSRKFTRVALQFPDNLLKDSIRIVRALRAKLGSLRSSDGERGVDD 61

Query: 1033 KDIGLYVMADTTYGSCCVDEVGASHINADCVIHYGHTCLSPTSNLPAFFVFGKAPINASN 854
            KDIGL+VMADTTYGSCCVDEVGASH++ADCVIHYGHTCLSPTS LPAFFVFGKA IN SN
Sbjct: 62   KDIGLFVMADTTYGSCCVDEVGASHVDADCVIHYGHTCLSPTSTLPAFFVFGKASINTSN 121

Query: 853  CAKHLCDYASSTTKPIMVLFGLEYGHALQDVIEASNVETTHMSGDTSKIQLHFAEVMGQS 674
            C ++L  +A +  KPI+VL+GLEY HA+  + EA  V ++   G   K++++FA+VM   
Sbjct: 122  CIENLSKHALTNGKPILVLYGLEYAHAIPHIREAVKVASSSY-GSGLKLEINFADVMCTV 180

Query: 673  MIPTNSSETNCSRCTADGDCGDDAVKRKYRIGGLVWNLEEGQNMEDYSLFWIGPDNSAFA 494
            + P   S+ + S C   G C       ++ IGGLVWN+ + + ME++ LFWIG DNSAFA
Sbjct: 181  ITP---SKDHKSLCGPAGGC------TRHTIGGLVWNIPDRKKMEEHLLFWIGSDNSAFA 231

Query: 493  NAVLTFNGCDIVRYDATANQLLTDTSQQRRILKRRYYLVEKAKDAAIVGILVGTLGVAGY 314
            N VLTFNGC+IVRYDAT  +LLTD SQ  +ILKRRYYLVEKAKDA I+G+LVGTLGVAGY
Sbjct: 232  NVVLTFNGCEIVRYDATEERLLTDVSQPLKILKRRYYLVEKAKDANIIGVLVGTLGVAGY 291

Query: 313  LNMIHQIKEMITRAGKKAYMLVMGKPNPSKLANFPECNVFIYISCAQTALLDSKDFLAPV 134
            L+MIHQ+KE+IT+AGKKAY LVMGKPNP+KLANFPEC+VFI +SCAQTALLDSK+FLAPV
Sbjct: 292  LHMIHQMKELITKAGKKAYTLVMGKPNPAKLANFPECDVFINVSCAQTALLDSKEFLAPV 351

Query: 133  ITPFEAILAFNRGSEWTGKYVMEFQDLVASTTVGTEITKKEARF 2
            ITPFEA+LAF RG++WTG YVMEF+DL++S+ V      +EARF
Sbjct: 352  ITPFEAMLAFGRGTQWTGAYVMEFRDLMSSSPVEGSDQAEEARF 395