BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00020942
         (667 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_022006098.1| pullulanase 1, chloroplastic isoform X2 [Hel...   419   e-138
ref|XP_022006097.1| pullulanase 1, chloroplastic isoform X1 [Hel...   419   e-138
gb|KVI09675.1| Alpha-1,6-glucosidase, pullulanase-type, C-termin...   414   e-136
ref|XP_023751382.1| pullulanase 1, chloroplastic [Lactuca sativa...   413   e-135
gb|OMO65719.1| hypothetical protein CCACVL1_21427 [Corchorus cap...   372   e-128
gb|PHT99619.1| Pullulanase 1, chloroplastic [Capsicum chinense]       378   e-128
dbj|GAU16971.1| hypothetical protein TSUD_37290, partial [Trifol...   371   e-127
gb|ONI23745.1| hypothetical protein PRUPE_2G205800 [Prunus persica]   389   e-126
gb|PON33464.1| Glycoside hydrolase [Parasponia andersonii]            389   e-126
gb|KCW65289.1| hypothetical protein EUGRSUZ_G02746 [Eucalyptus g...   385   e-126
ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica...   389   e-126
gb|ONI23748.1| hypothetical protein PRUPE_2G205800 [Prunus persica]   389   e-126
ref|XP_024025960.1| pullulanase 1, chloroplastic [Morus notabilis]    387   e-125
dbj|BAT94615.1| hypothetical protein VIGAN_08123400 [Vigna angul...   373   e-125
ref|XP_018715184.1| PREDICTED: pullulanase 1, chloroplastic isof...   385   e-125
ref|XP_008233250.1| PREDICTED: pullulanase 1, chloroplastic [Pru...   385   e-125
ref|XP_010067209.1| PREDICTED: pullulanase 1, chloroplastic isof...   385   e-125
ref|XP_010067208.1| PREDICTED: pullulanase 1, chloroplastic isof...   385   e-125
gb|OVA17964.1| Glycoside hydrolase [Macleaya cordata]                 385   e-125
gb|PIA37162.1| hypothetical protein AQUCO_03000024v1, partial [A...   384   e-124

>ref|XP_022006098.1| pullulanase 1, chloroplastic isoform X2 [Helianthus annuus]
          Length = 951

 Score =  419 bits (1076), Expect = e-138
 Identities = 198/222 (89%), Positives = 213/222 (95%)
 Frame = +2

Query: 2    GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
            GGSPVAYA+QPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV
Sbjct: 686  GGSPVAYATQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 745

Query: 182  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
            PFFHCGDEILRSKSLDRDSYNSGDWFNR+DFSYNSNNWGVGLPPKGKNEKSWP+IKPRLA
Sbjct: 746  PFFHCGDEILRSKSLDRDSYNSGDWFNRIDFSYNSNNWGVGLPPKGKNEKSWPLIKPRLA 805

Query: 362  SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
            S SFKP KSHI+SALENFQNLL IRYSS+LFRLRTANAIQERVRFHNTG +HVPGVIVMS
Sbjct: 806  SASFKPNKSHILSALENFQNLLLIRYSSVLFRLRTANAIQERVRFHNTGPSHVPGVIVMS 865

Query: 542  IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            IEDGH+G+PGLT+LDPIYSY++++ N+ P  +SF SPVLRGK
Sbjct: 866  IEDGHEGVPGLTRLDPIYSYIVVIFNSCPTGISFASPVLRGK 907


>ref|XP_022006097.1| pullulanase 1, chloroplastic isoform X1 [Helianthus annuus]
 gb|OTF99361.1| putative limit dextrinase [Helianthus annuus]
          Length = 952

 Score =  419 bits (1076), Expect = e-138
 Identities = 198/222 (89%), Positives = 213/222 (95%)
 Frame = +2

Query: 2    GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
            GGSPVAYA+QPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV
Sbjct: 687  GGSPVAYATQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 746

Query: 182  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
            PFFHCGDEILRSKSLDRDSYNSGDWFNR+DFSYNSNNWGVGLPPKGKNEKSWP+IKPRLA
Sbjct: 747  PFFHCGDEILRSKSLDRDSYNSGDWFNRIDFSYNSNNWGVGLPPKGKNEKSWPLIKPRLA 806

Query: 362  SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
            S SFKP KSHI+SALENFQNLL IRYSS+LFRLRTANAIQERVRFHNTG +HVPGVIVMS
Sbjct: 807  SASFKPNKSHILSALENFQNLLLIRYSSVLFRLRTANAIQERVRFHNTGPSHVPGVIVMS 866

Query: 542  IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            IEDGH+G+PGLT+LDPIYSY++++ N+ P  +SF SPVLRGK
Sbjct: 867  IEDGHEGVPGLTRLDPIYSYIVVIFNSCPTGISFASPVLRGK 908


>gb|KVI09675.1| Alpha-1,6-glucosidase, pullulanase-type, C-terminal [Cynara
            cardunculus var. scolymus]
          Length = 908

 Score =  414 bits (1063), Expect = e-136
 Identities = 196/222 (88%), Positives = 211/222 (95%)
 Frame = +2

Query: 2    GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
            GGSPV YASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATS+IALSQGV
Sbjct: 649  GGSPVGYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSVIALSQGV 708

Query: 182  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
            PFFHCGDEILRSKS+DRDSYNSGDWFNRLDFSY+SNNWGVGLPPKGKNE SWPIIKPRLA
Sbjct: 709  PFFHCGDEILRSKSMDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNENSWPIIKPRLA 768

Query: 362  SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
            SP FKPQKSHI+SALENFQNLLRIRYSSIL RLRTANAIQERVRFHNTG + +PGVIVMS
Sbjct: 769  SPLFKPQKSHILSALENFQNLLRIRYSSILLRLRTANAIQERVRFHNTGVSQIPGVIVMS 828

Query: 542  IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            IEDGH+G+ GL+QLDP+YSY+++VIN  P ++SF SPVLRGK
Sbjct: 829  IEDGHEGVAGLSQLDPMYSYIVVVINVCPTEISFASPVLRGK 870


>ref|XP_023751382.1| pullulanase 1, chloroplastic [Lactuca sativa]
 gb|PLY94815.1| hypothetical protein LSAT_2X103740 [Lactuca sativa]
          Length = 963

 Score =  413 bits (1062), Expect = e-135
 Identities = 197/222 (88%), Positives = 210/222 (94%)
 Frame = +2

Query: 2    GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
            GG PVAY  QPVETINYVSAHDNETLFDIISLK PMEIS+DERCRINHLATSIIALSQGV
Sbjct: 696  GGDPVAYTLQPVETINYVSAHDNETLFDIISLKAPMEISIDERCRINHLATSIIALSQGV 755

Query: 182  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
            PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPK KNEKSWPIIKPRLA
Sbjct: 756  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEKSWPIIKPRLA 815

Query: 362  SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
            SPSFKP K+HI+SALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTG + VPGVIVM+
Sbjct: 816  SPSFKPHKNHILSALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGGSQVPGVIVMT 875

Query: 542  IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            IEDGH+G+PGLTQLDP Y+Y+++VINA P ++SFTSPV RGK
Sbjct: 876  IEDGHEGVPGLTQLDPEYAYIVVVINACPTEISFTSPVFRGK 917


>gb|OMO65719.1| hypothetical protein CCACVL1_21427 [Corchorus capsularis]
          Length = 272

 Score =  372 bits (956), Expect = e-128
 Identities = 175/222 (78%), Positives = 200/222 (90%)
 Frame = +2

Query: 2   GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
           GG+PVAYA  P ET+NYVSAHDNETLFDIISLKTP+EISV+ERCRINH ATS+IALSQG+
Sbjct: 3   GGTPVAYALCPTETVNYVSAHDNETLFDIISLKTPVEISVEERCRINHFATSVIALSQGI 62

Query: 182 PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
           PFFH GDE+LRSKSLDRDSYNSGDWFNRLDF+YNSNNWGVGLPPK KNEK+W +I+PRLA
Sbjct: 63  PFFHAGDEMLRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKEKNEKNWSLIQPRLA 122

Query: 362 SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
            PSFKPQ+SHI++A ENF NLLRIRYSS LFRLRTANAIQERVRFHNTG + VPG+IVMS
Sbjct: 123 DPSFKPQRSHILAAAENFMNLLRIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGIIVMS 182

Query: 542 IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
           IEDG++G+PGL+QLD  +SYV+++ N  P + SF SP LRGK
Sbjct: 183 IEDGNEGVPGLSQLDRNFSYVVVIFNPCPTEASFFSPTLRGK 224


>gb|PHT99619.1| Pullulanase 1, chloroplastic [Capsicum chinense]
          Length = 436

 Score =  378 bits (970), Expect = e-128
 Identities = 173/221 (78%), Positives = 202/221 (91%)
 Frame = +2

Query: 5   GSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGVP 184
           G PV YAS P+ET+NYVSAHDNETLFDIISLKTP +ISV+ERCR+NHLATS+IALSQG+P
Sbjct: 174 GKPVGYASSPIETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSVIALSQGIP 233

Query: 185 FFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLAS 364
           FFH GDE+LRSKS+DRDSYNSGDWFNRLDFSYNSNNWGVGLPPK KNE++WP+IKPRLA 
Sbjct: 234 FFHAGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNERNWPLIKPRLAD 293

Query: 365 PSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMSI 544
           PS+KPQKSHI++A+ENF NL++IRYSS LFRLRTAN+IQERVRFHNTG + +PG+IVMSI
Sbjct: 294 PSYKPQKSHILAAVENFLNLMQIRYSSPLFRLRTANSIQERVRFHNTGPSWIPGLIVMSI 353

Query: 545 EDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
           EDGH  +PGL+QLDPIYSY++++IN  P DVSF +P LR K
Sbjct: 354 EDGHQEVPGLSQLDPIYSYIVVIINPCPTDVSFANPALREK 394


>dbj|GAU16971.1| hypothetical protein TSUD_37290, partial [Trifolium subterraneum]
          Length = 320

 Score =  371 bits (953), Expect = e-127
 Identities = 174/222 (78%), Positives = 200/222 (90%)
 Frame = +2

Query: 2   GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
           GG+PVA+AS P+ET+NYVSAHDNETLFDI+S KTPM+I V ERCRINHLATSIIALSQG+
Sbjct: 53  GGTPVAFASFPIETVNYVSAHDNETLFDIVSFKTPMDIGVAERCRINHLATSIIALSQGI 112

Query: 182 PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
           PFFH GDEILRSKSLDRDSYNSGDWFNRLDF+YNSNNWGVGLPP+ KNEKSWP+IKPRLA
Sbjct: 113 PFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPQEKNEKSWPLIKPRLA 172

Query: 362 SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
            PSF+PQ++ I++ +ENF NLLRIRYSS LFRLRTANAIQ+RVRFHNTG +   GVIVMS
Sbjct: 173 DPSFRPQRTDILATMENFLNLLRIRYSSPLFRLRTANAIQQRVRFHNTGPSLAYGVIVMS 232

Query: 542 IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
           IEDGHDG PGL+QLDPIYS++++V N SP +VSF SP L+ +
Sbjct: 233 IEDGHDGFPGLSQLDPIYSFIVVVFNVSPQEVSFVSPSLQSR 274


>gb|ONI23745.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 939

 Score =  389 bits (998), Expect = e-126
 Identities = 183/222 (82%), Positives = 205/222 (92%)
 Frame = +2

Query: 2    GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
            GG+PVAY   P ETINYVSAHDNETLFDI+SLKTPMEISV+ERCRINHLATSIIAL+QG+
Sbjct: 674  GGTPVAYTLCPTETINYVSAHDNETLFDIVSLKTPMEISVEERCRINHLATSIIALAQGI 733

Query: 182  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
            PFFH GDEILRSKSLDRDSYNSGDWFNRLDF+Y+SNNWGVGLPPK KNEKSWP+ KPRLA
Sbjct: 734  PFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPLFKPRLA 793

Query: 362  SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
             PSFKPQKSHII+A+ENF NLLRIRYSS LFRLRTANAIQERVRFHNTG + VPGVIVMS
Sbjct: 794  DPSFKPQKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQERVRFHNTGPSLVPGVIVMS 853

Query: 542  IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            IEDGH+G+PGL+QLDPIYSY+++++NA P +VSF SP L+ +
Sbjct: 854  IEDGHEGVPGLSQLDPIYSYIVVIVNACPTEVSFASPSLQAR 895


>gb|PON33464.1| Glycoside hydrolase [Parasponia andersonii]
          Length = 973

 Score =  389 bits (1000), Expect = e-126
 Identities = 183/220 (83%), Positives = 204/220 (92%)
 Frame = +2

Query: 8    SPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGVPF 187
            SPV YAS P ET+NYVSAHDNETLFDIISLKTPM ISVDERCRINHLATSIIALSQG+PF
Sbjct: 710  SPVGYASHPTETVNYVSAHDNETLFDIISLKTPMGISVDERCRINHLATSIIALSQGIPF 769

Query: 188  FHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLASP 367
            FH GDE+LRSKSLDRDSYNSGDWFNRLDF+YNSNNWGVGLPPK KNEKSWP+IKPRLA P
Sbjct: 770  FHSGDEMLRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKEKNEKSWPLIKPRLADP 829

Query: 368  SFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMSIE 547
             FKPQ+SHI+SA+ENF +LLRIRYSS LFRLRT NA+QERVRFHNTG++ +PGVIVMSIE
Sbjct: 830  LFKPQRSHILSAVENFSDLLRIRYSSPLFRLRTENAVQERVRFHNTGSSSIPGVIVMSIE 889

Query: 548  DGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            DGH+G+PGL+QLDPIYSYV+++INA P +VSFTSP LR +
Sbjct: 890  DGHEGLPGLSQLDPIYSYVVVIINARPTEVSFTSPALRAR 929


>gb|KCW65289.1| hypothetical protein EUGRSUZ_G02746 [Eucalyptus grandis]
          Length = 816

 Score =  385 bits (989), Expect = e-126
 Identities = 179/221 (80%), Positives = 204/221 (92%)
 Frame = +2

Query: 5    GSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGVP 184
            GSPVAYA  P ET+NYVSAHDNETLFDI+SLKTPM ISVDERCR+NHLATSIIAL+QGVP
Sbjct: 552  GSPVAYALSPTETVNYVSAHDNETLFDIVSLKTPMSISVDERCRMNHLATSIIALAQGVP 611

Query: 185  FFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLAS 364
            FFHCGDE+LRSKSLDRDSYNSGDWFNRLDF+YN +NWGVGLPPK KNEK+WP+IKPRLA 
Sbjct: 612  FFHCGDELLRSKSLDRDSYNSGDWFNRLDFTYNDSNWGVGLPPKEKNEKNWPLIKPRLAD 671

Query: 365  PSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMSI 544
            PSFKPQKS+I++A++NF N+LRIRYSS LFRL+TANAIQERVRF NTG + +PGVIVMSI
Sbjct: 672  PSFKPQKSNILAAVDNFLNMLRIRYSSPLFRLKTANAIQERVRFLNTGPSQIPGVIVMSI 731

Query: 545  EDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            EDGH+G+PGLTQLDPIYSY+ +VIN  P++VSF SP L+GK
Sbjct: 732  EDGHEGVPGLTQLDPIYSYIAIVINTGPSEVSFASPALQGK 772


>ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica]
 gb|ONI23747.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 965

 Score =  389 bits (998), Expect = e-126
 Identities = 183/222 (82%), Positives = 205/222 (92%)
 Frame = +2

Query: 2    GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
            GG+PVAY   P ETINYVSAHDNETLFDI+SLKTPMEISV+ERCRINHLATSIIAL+QG+
Sbjct: 700  GGTPVAYTLCPTETINYVSAHDNETLFDIVSLKTPMEISVEERCRINHLATSIIALAQGI 759

Query: 182  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
            PFFH GDEILRSKSLDRDSYNSGDWFNRLDF+Y+SNNWGVGLPPK KNEKSWP+ KPRLA
Sbjct: 760  PFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPLFKPRLA 819

Query: 362  SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
             PSFKPQKSHII+A+ENF NLLRIRYSS LFRLRTANAIQERVRFHNTG + VPGVIVMS
Sbjct: 820  DPSFKPQKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQERVRFHNTGPSLVPGVIVMS 879

Query: 542  IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            IEDGH+G+PGL+QLDPIYSY+++++NA P +VSF SP L+ +
Sbjct: 880  IEDGHEGVPGLSQLDPIYSYIVVIVNACPTEVSFASPSLQAR 921


>gb|ONI23748.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 966

 Score =  389 bits (998), Expect = e-126
 Identities = 183/222 (82%), Positives = 205/222 (92%)
 Frame = +2

Query: 2    GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
            GG+PVAY   P ETINYVSAHDNETLFDI+SLKTPMEISV+ERCRINHLATSIIAL+QG+
Sbjct: 701  GGTPVAYTLCPTETINYVSAHDNETLFDIVSLKTPMEISVEERCRINHLATSIIALAQGI 760

Query: 182  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
            PFFH GDEILRSKSLDRDSYNSGDWFNRLDF+Y+SNNWGVGLPPK KNEKSWP+ KPRLA
Sbjct: 761  PFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPLFKPRLA 820

Query: 362  SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
             PSFKPQKSHII+A+ENF NLLRIRYSS LFRLRTANAIQERVRFHNTG + VPGVIVMS
Sbjct: 821  DPSFKPQKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQERVRFHNTGPSLVPGVIVMS 880

Query: 542  IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            IEDGH+G+PGL+QLDPIYSY+++++NA P +VSF SP L+ +
Sbjct: 881  IEDGHEGVPGLSQLDPIYSYIVVIVNACPTEVSFASPSLQAR 922


>ref|XP_024025960.1| pullulanase 1, chloroplastic [Morus notabilis]
          Length = 964

 Score =  387 bits (993), Expect = e-125
 Identities = 182/221 (82%), Positives = 205/221 (92%)
 Frame = +2

Query: 5    GSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGVP 184
            GSPVAYAS P ETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQG+P
Sbjct: 700  GSPVAYASCPTETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGIP 759

Query: 185  FFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLAS 364
            FFH GDE+LRSKSLDRDSYNSGDWFNRLDF+YNSNNWGVGLPP+ KNE +WP+IKPRLA 
Sbjct: 760  FFHSGDEMLRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPRAKNETNWPLIKPRLAE 819

Query: 365  PSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMSI 544
             SFKPQ++HI++A+ENF NLLRIRYSS LFRL+TANAIQERVRFHNTG++ +PGVIVMSI
Sbjct: 820  SSFKPQRNHILAAVENFSNLLRIRYSSPLFRLKTANAIQERVRFHNTGSSSIPGVIVMSI 879

Query: 545  EDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            EDGH+G+PGL+QLDPIYSY+ ++INA P  VSF+SP LR +
Sbjct: 880  EDGHEGLPGLSQLDPIYSYIAVIINACPTVVSFSSPTLRAR 920


>dbj|BAT94615.1| hypothetical protein VIGAN_08123400 [Vigna angularis var.
           angularis]
          Length = 502

 Score =  373 bits (957), Expect = e-125
 Identities = 175/221 (79%), Positives = 201/221 (90%)
 Frame = +2

Query: 5   GSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGVP 184
           G+PVAYAS P ETINYVSAHDNETLFDI+SLKTPM+I+V ERCRINHLATSIIALSQG+P
Sbjct: 238 GTPVAYASCPTETINYVSAHDNETLFDIVSLKTPMDITVTERCRINHLATSIIALSQGIP 297

Query: 185 FFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLAS 364
           FFHCGDEILRSKSLDRDSYNSGDWFNRLDF+YNSNNWGVGLPP+ KNEK+WP+IKPRLA+
Sbjct: 298 FFHCGDEILRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPQEKNEKNWPLIKPRLAN 357

Query: 365 PSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMSI 544
           PSFKPQK  I++ ++NF NLLRIRYSS LFRL+TANAIQ+RV FHNTG + V GVIVMSI
Sbjct: 358 PSFKPQKIDILATMDNFLNLLRIRYSSPLFRLKTANAIQQRVSFHNTGPSWVCGVIVMSI 417

Query: 545 EDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
           EDGHDG PGL+QLDPIYS++++V+NASP + SF  P L+ +
Sbjct: 418 EDGHDGFPGLSQLDPIYSFIVVVMNASPKEASFVCPALQSR 458


>ref|XP_018715184.1| PREDICTED: pullulanase 1, chloroplastic isoform X3 [Eucalyptus
            grandis]
          Length = 951

 Score =  385 bits (989), Expect = e-125
 Identities = 179/221 (80%), Positives = 204/221 (92%)
 Frame = +2

Query: 5    GSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGVP 184
            GSPVAYA  P ET+NYVSAHDNETLFDI+SLKTPM ISVDERCR+NHLATSIIAL+QGVP
Sbjct: 687  GSPVAYALSPTETVNYVSAHDNETLFDIVSLKTPMSISVDERCRMNHLATSIIALAQGVP 746

Query: 185  FFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLAS 364
            FFHCGDE+LRSKSLDRDSYNSGDWFNRLDF+YN +NWGVGLPPK KNEK+WP+IKPRLA 
Sbjct: 747  FFHCGDELLRSKSLDRDSYNSGDWFNRLDFTYNDSNWGVGLPPKEKNEKNWPLIKPRLAD 806

Query: 365  PSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMSI 544
            PSFKPQKS+I++A++NF N+LRIRYSS LFRL+TANAIQERVRF NTG + +PGVIVMSI
Sbjct: 807  PSFKPQKSNILAAVDNFLNMLRIRYSSPLFRLKTANAIQERVRFLNTGPSQIPGVIVMSI 866

Query: 545  EDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            EDGH+G+PGLTQLDPIYSY+ +VIN  P++VSF SP L+GK
Sbjct: 867  EDGHEGVPGLTQLDPIYSYIAIVINTGPSEVSFASPALQGK 907


>ref|XP_008233250.1| PREDICTED: pullulanase 1, chloroplastic [Prunus mume]
          Length = 967

 Score =  385 bits (990), Expect = e-125
 Identities = 181/222 (81%), Positives = 204/222 (91%)
 Frame = +2

Query: 2    GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
            GG+PVAY   P ETINYVSAHDNETLFDI+SLKTPMEISV+ERCRINHLATSIIAL+QG+
Sbjct: 702  GGTPVAYTLCPTETINYVSAHDNETLFDIVSLKTPMEISVEERCRINHLATSIIALAQGI 761

Query: 182  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
            PFFH GDEILRSKSLDRDSYNSGDWFNRLDF+Y+SNNWGVGLPPK KNEKSWP+ KPRLA
Sbjct: 762  PFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPLFKPRLA 821

Query: 362  SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
             PSFKPQ+SHII+A+ENF NLL IRYSS LFRLRTANAIQERVRFHNTG + VPGVIVMS
Sbjct: 822  DPSFKPQRSHIIAAVENFSNLLHIRYSSPLFRLRTANAIQERVRFHNTGPSLVPGVIVMS 881

Query: 542  IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            IEDGH+G+PGL+QLDPIYSY+++++NA P +VSF SP L+ +
Sbjct: 882  IEDGHEGVPGLSQLDPIYSYIVVIVNACPTEVSFASPSLQAR 923


>ref|XP_010067209.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Eucalyptus
            grandis]
          Length = 952

 Score =  385 bits (989), Expect = e-125
 Identities = 179/221 (80%), Positives = 204/221 (92%)
 Frame = +2

Query: 5    GSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGVP 184
            GSPVAYA  P ET+NYVSAHDNETLFDI+SLKTPM ISVDERCR+NHLATSIIAL+QGVP
Sbjct: 688  GSPVAYALSPTETVNYVSAHDNETLFDIVSLKTPMSISVDERCRMNHLATSIIALAQGVP 747

Query: 185  FFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLAS 364
            FFHCGDE+LRSKSLDRDSYNSGDWFNRLDF+YN +NWGVGLPPK KNEK+WP+IKPRLA 
Sbjct: 748  FFHCGDELLRSKSLDRDSYNSGDWFNRLDFTYNDSNWGVGLPPKEKNEKNWPLIKPRLAD 807

Query: 365  PSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMSI 544
            PSFKPQKS+I++A++NF N+LRIRYSS LFRL+TANAIQERVRF NTG + +PGVIVMSI
Sbjct: 808  PSFKPQKSNILAAVDNFLNMLRIRYSSPLFRLKTANAIQERVRFLNTGPSQIPGVIVMSI 867

Query: 545  EDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            EDGH+G+PGLTQLDPIYSY+ +VIN  P++VSF SP L+GK
Sbjct: 868  EDGHEGVPGLTQLDPIYSYIAIVINTGPSEVSFASPALQGK 908


>ref|XP_010067208.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Eucalyptus
            grandis]
 ref|XP_018715183.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Eucalyptus
            grandis]
          Length = 953

 Score =  385 bits (989), Expect = e-125
 Identities = 179/221 (80%), Positives = 204/221 (92%)
 Frame = +2

Query: 5    GSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGVP 184
            GSPVAYA  P ET+NYVSAHDNETLFDI+SLKTPM ISVDERCR+NHLATSIIAL+QGVP
Sbjct: 689  GSPVAYALSPTETVNYVSAHDNETLFDIVSLKTPMSISVDERCRMNHLATSIIALAQGVP 748

Query: 185  FFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLAS 364
            FFHCGDE+LRSKSLDRDSYNSGDWFNRLDF+YN +NWGVGLPPK KNEK+WP+IKPRLA 
Sbjct: 749  FFHCGDELLRSKSLDRDSYNSGDWFNRLDFTYNDSNWGVGLPPKEKNEKNWPLIKPRLAD 808

Query: 365  PSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMSI 544
            PSFKPQKS+I++A++NF N+LRIRYSS LFRL+TANAIQERVRF NTG + +PGVIVMSI
Sbjct: 809  PSFKPQKSNILAAVDNFLNMLRIRYSSPLFRLKTANAIQERVRFLNTGPSQIPGVIVMSI 868

Query: 545  EDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            EDGH+G+PGLTQLDPIYSY+ +VIN  P++VSF SP L+GK
Sbjct: 869  EDGHEGVPGLTQLDPIYSYIAIVINTGPSEVSFASPALQGK 909


>gb|OVA17964.1| Glycoside hydrolase [Macleaya cordata]
          Length = 983

 Score =  385 bits (990), Expect = e-125
 Identities = 181/221 (81%), Positives = 202/221 (91%)
 Frame = +2

Query: 5    GSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGVP 184
            G PVAYA  P+ET+NYVSAHDNETLFDIISLKTPMEISVDERCR+NHLATSIIALSQGVP
Sbjct: 719  GLPVAYALCPIETVNYVSAHDNETLFDIISLKTPMEISVDERCRLNHLATSIIALSQGVP 778

Query: 185  FFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLAS 364
            FFHCGDE+LRSKSLDRDSYNSGDWFNRLDFSY +NNWGVGLPPKGKNEK+W +IKPRLA 
Sbjct: 779  FFHCGDEMLRSKSLDRDSYNSGDWFNRLDFSYETNNWGVGLPPKGKNEKNWSLIKPRLAD 838

Query: 365  PSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMSI 544
            PSFKPQK+HI++A+ENF NLLRIRYSS LFRLRTANA+QERVRFHNTG + VPG+IVMSI
Sbjct: 839  PSFKPQKNHILTAVENFLNLLRIRYSSPLFRLRTANAVQERVRFHNTGPSLVPGIIVMSI 898

Query: 545  EDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            EDGH+G PGL QLDP YSY+++  NA P +V+FTSP LR +
Sbjct: 899  EDGHEGKPGLAQLDPTYSYIVVAFNACPGEVTFTSPALRAR 939


>gb|PIA37162.1| hypothetical protein AQUCO_03000024v1, partial [Aquilegia coerulea]
          Length = 951

 Score =  384 bits (987), Expect = e-124
 Identities = 177/222 (79%), Positives = 204/222 (91%)
 Frame = +2

Query: 2    GGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLATSIIALSQGV 181
            GG PVAYAS P+ETINY SAHDNETLFDIISLKTP++ISVD+RCR+NHLATSI+ALSQG+
Sbjct: 685  GGIPVAYASSPIETINYASAHDNETLFDIISLKTPIKISVDDRCRMNHLATSIVALSQGI 744

Query: 182  PFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLA 361
            PFFHCGDE+LRSKSLDRDSYNSGDWFNR+DF+Y SNNWGVGLPPKGKNEKSWP+IKPRLA
Sbjct: 745  PFFHCGDEMLRSKSLDRDSYNSGDWFNRIDFTYESNNWGVGLPPKGKNEKSWPLIKPRLA 804

Query: 362  SPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNTGTAHVPGVIVMS 541
             PSFKP K HI++A+ENF NLLRIRYSS L RLRTANAIQ+RV+FHNTG + VPG+IVMS
Sbjct: 805  DPSFKPNKGHILAAVENFLNLLRIRYSSPLLRLRTANAIQKRVQFHNTGPSWVPGLIVMS 864

Query: 542  IEDGHDGIPGLTQLDPIYSYVLLVINASPNDVSFTSPVLRGK 667
            IEDGH+G+PGL QLDP+YSY+++  NA PN+V FTSPVL+ +
Sbjct: 865  IEDGHEGMPGLPQLDPVYSYIVVAFNACPNEVLFTSPVLQAR 906