BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00019886
         (3509 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_023730599.1| separase [Lactuca sativa] >gi|1322379086|gb|...   719   0.0  
gb|KVH93464.1| Peptidase C50, separase [Cynara cardunculus var. ...   534   e-159
ref|XP_021988126.1| separase [Helianthus annuus] >gi|1191658716|...   484   e-142
ref|XP_020997748.1| separase-like [Arachis duranensis]                419   e-126
ref|XP_021285607.1| separase isoform X4 [Herrania umbratica] >gi...   420   e-120
ref|XP_022942952.1| separase isoform X2 [Cucurbita moschata]          422   e-120
ref|XP_022748572.1| separase isoform X2 [Durio zibethinus]            421   e-120
ref|XP_021285606.1| separase isoform X3 [Herrania umbratica]          420   e-119
ref|XP_021285605.1| separase isoform X2 [Herrania umbratica]          420   e-119
ref|XP_021285604.1| separase isoform X1 [Herrania umbratica]          420   e-119
ref|XP_022942953.1| separase isoform X3 [Cucurbita moschata]          418   e-119
gb|KJB11859.1| hypothetical protein B456_002G207900 [Gossypium r...   416   e-119
gb|POE83410.1| separase [Quercus suber]                               415   e-119
ref|XP_022748575.1| separase isoform X4 [Durio zibethinus]            417   e-119
ref|XP_022748574.1| separase isoform X3 [Durio zibethinus]            417   e-119
ref|XP_022748571.1| separase isoform X1 [Durio zibethinus]            417   e-119
ref|XP_016711479.1| PREDICTED: separase-like [Gossypium hirsutum]     416   e-118
gb|EOX97396.1| Separase, putative [Theobroma cacao]                   416   e-118
ref|XP_017971181.1| PREDICTED: separase isoform X2 [Theobroma ca...   416   e-118
ref|XP_017971180.1| PREDICTED: separase isoform X1 [Theobroma ca...   416   e-118

>ref|XP_023730599.1| separase [Lactuca sativa]
 gb|PLY76336.1| hypothetical protein LSAT_5X109460 [Lactuca sativa]
          Length = 2079

 Score =  719 bits (1856), Expect = 0.0
 Identities = 466/1178 (39%), Positives = 639/1178 (54%), Gaps = 161/1178 (13%)
 Frame = +2

Query: 53   SVEFWKHCMENLEP-RIGFEQKLSILPTISSPDSFQHDHAI--HINIEEVKQAATNLIDR 223
            S+EFW  CM+  +   +GF+Q LS++ T+SSPDS + +HAI  HI  +EVKQAA++LID 
Sbjct: 908  SMEFWISCMKGSKSLEVGFQQSLSLISTLSSPDSCKRNHAIQPHITTDEVKQAASDLIDN 967

Query: 224  DPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICH-SKKVDK 400
             PLS +S F+A HLYYDL E M+ +G MI            RTKLL K F+    ++ D 
Sbjct: 968  VPLSTNSLFLAAHLYYDLGERMIAQGLMIEALCYAKEAHRLRTKLLQKSFMYSIEQQNDM 1027

Query: 401  VFDDNGEVIEI-KYTYKTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFLESILQI 577
            V  ++G+ I+  KY  KTF M  SVA +AWS +K S + +D ++TPWNVL+ +LES LQI
Sbjct: 1028 VGVNDGDTIQKHKYVLKTFHMHPSVATSAWSSEKGSFDFEDCVVTPWNVLRVYLESTLQI 1087

Query: 578  GNL---IDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSEFQLQSA 748
            G L   + +   A+++L+ GK I+  Q L IF + F   L KLY KQ  W ++E +L+SA
Sbjct: 1088 GTLQEIVGNGSEAESLLLWGKNIATFQSLPIFIVGFSCVLGKLYGKQHHWHLAEKELESA 1147

Query: 749  LSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFNNKSKILKELDDADSFYKDVEKVL 928
              +   S   +SC+ C+ VL+V+I Q   DL+  RF + +K+L+ L  A++FY+     L
Sbjct: 1148 KHMLADSCRLVSCLKCRLVLQVTINQQLGDLFRIRFKSTNKLLEGLSKAEAFYRSATDKL 1207

Query: 929  NEPIWENDISSPEKSSARKTVFCDA--IGIPFALQGNEEN-------------------- 1042
                W+N +S  E+SSAR T+FCDA  IG   A Q +E                      
Sbjct: 1208 KVSEWKNCVSDCEESSARNTMFCDALSIGENQAEQNDESQINGKETIRPKVTRKSKKSAK 1267

Query: 1043 -----------------------------CFHCQPLKVKKSMSLQMLIHLKMECXXXXXX 1135
                                         C+HC P +V KS SL  ++ +K EC      
Sbjct: 1268 PLPEKNTTSRITRSSKQRGEVTCVSGEGKCWHCLPSEVMKSKSLTNILQMKWECIRRRLL 1327

Query: 1136 XXXXXXXXXXHRARGEINDEQKV-LYSISVLFSRCLFRESHFPISPEFVTTMIDQNLTGL 1312
                         RGEI    +V + SISVL  R  F + HF +S  F+  +I++N+ G 
Sbjct: 1328 LRLLTGIGKCLGIRGEIQRAHEVFMESISVLVHRSTFHQPHFSVSIAFLAELIEKNVIGD 1387

Query: 1313 AFGVEHTSILYKICWHFLKCISNKDT----SKKSIIATRTIVRGLKIAFIRTRLVPELHQ 1480
             F VEH SILY ICW  LK + +K T    S  S I   TIV GLK +FI  R VP L Q
Sbjct: 1388 VFAVEHASILYNICWFSLKSLCDKGTRHDGSDMSTIPITTIVSGLKSSFILCREVPVLFQ 1447

Query: 1481 KVARSLAVLY---ASDEALSKCDSPCV----NQWAAYFHQASIGTYLNNHYSDRHQDHIH 1639
            KV+R LA+LY   +S+ ALS   S       +QWA++FHQAS+GT+LN+    R   H  
Sbjct: 1448 KVSRLLAMLYTLSSSNNALSMLSSSSSVLSESQWASFFHQASLGTHLNHQLVSRIGKHKD 1507

Query: 1640 QEP---------VKVEKIEKDLEASVLEFFSNLPNVAILCISMLEDD-SDFLSNLLPHSP 1789
            Q           V  E I  DLE  VL+F+  LP   I+CISML DD +  L  LLP++P
Sbjct: 1508 QNTTTDIHGLLRVAPESI-LDLEGFVLKFYKGLPCTTIICISMLGDDYTTLLRELLPYNP 1566

Query: 1790 -TSVWIVLSRLNSNDVPIFVMLPVASTF-------QDSAGFPINKDCSVRRPWDCPWVGD 1945
             T  WI+LSRLNS+ +PI  +LP+ S         +DS+   ++   S  + W CPW G 
Sbjct: 1567 STHAWIILSRLNSDTIPIITLLPINSILTESSEGNEDSSSSFLSNKKSCNKSWHCPW-GH 1625

Query: 1946 TVMDRAAPIFKSILEE---------FHTTDPNQSVIIN--KKLDQCLSELLSDIEKKWFG 2092
            T++D  AP+FK+ILEE            T  N+S+     +KLDQ L +LL D+E  WFG
Sbjct: 1626 TIVDNIAPLFKTILEENYISSSVYPLEDTKKNRSLWWGQRRKLDQLLGDLLRDLEDLWFG 1685

Query: 2093 RWKQLLWGEWSDYKNIVC---KLKDHLKSEY-INVDENILKVVLEGAG--VNTPKEEFLS 2254
             WK LL GE+SD K+I     KL + LK E  ++V E+ILKV++ GAG  V +  EE++S
Sbjct: 1686 PWKVLLLGEFSDNKHIDSVHKKLMNDLKFESKVDVHESILKVIIGGAGPHVASQHEEWVS 1745

Query: 2255 ELILSNGFYVGTTNSHCDGIDKDSFTSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQM 2434
            E+++  G YVG      + ID  S +S+S ++     +++++    R  VILVLD DIQM
Sbjct: 1746 EMMMKKGCYVGGIKCE-ERIDTLS-SSVSELILNAIREIEEE---DREAVILVLDFDIQM 1800

Query: 2435 LPWENLPILRHQDVSRMPSIMSISSTIRKKCSYDDNDHMVFPTIDPRDAYYVLNPQGDLK 2614
            LPWENLPILR+Q+V RMPS+ SI  T  + C Y++     FP IDP DAYY+LNP GDL 
Sbjct: 1801 LPWENLPILRNQEVYRMPSVASICYTFDRCCEYEEKSAF-FPMIDPLDAYYLLNPGGDLP 1859

Query: 2615 QLEHNLAEWFSKQKFEGIVGTSPTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDR 2794
              +     WF  Q  EG  GTSPT D+LSM LK  D+F+Y GHGSG   I GD ++ LD 
Sbjct: 1860 STQAEFQHWFKDQNLEGTTGTSPTVDELSMALKKHDLFMYFGHGSGVQYIPGDEIQKLDG 1919

Query: 2795 CAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLVKSL 2974
            CAA +L+GCSSGSL LNGSY P G  L YL AGSP +V +LW++T     +  K ++ + 
Sbjct: 1920 CAATLLMGCSSGSLSLNGSYSPKGAPLYYLFAGSPVIVANLWEVTDKDIDRFGKAVLDAW 1979

Query: 2975 MHSKD-----------------------------DKTTVGSSMGSARES----------- 3034
            + ++                              DK   G    S ++S           
Sbjct: 1980 IKTRSSSSTDCAQCTQVSISDRLNNMNIVDDDDGDKRKGGKKKTSRKKSVNNNNSNSNCK 2039

Query: 3035 ---------------CYFPYIQGASLICYGIKTRIKKR 3103
                           C  P++ GAS +CYG+ T I+ +
Sbjct: 2040 HRPKIGSFMGQAREACTLPFLIGASPVCYGVPTGIRNK 2077


>gb|KVH93464.1| Peptidase C50, separase [Cynara cardunculus var. scolymus]
          Length = 2176

 Score =  534 bits (1375), Expect = e-159
 Identities = 336/817 (41%), Positives = 443/817 (54%), Gaps = 126/817 (15%)
 Frame = +2

Query: 1031 NEENCFHCQPLKVKKSMSLQMLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKV-L 1207
            +E  C+HC P +V KS+SL+ +I  K EC                   RGEI    KV L
Sbjct: 1366 DEGKCWHCLPSEVMKSLSLRNIIQTKWECIRRRLLLRVLTGRGKCFGVRGEIQQAHKVFL 1425

Query: 1208 YSISVLFSRCLFRESHFPISPEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNKD 1387
             SISVL  R  F +SHF IS  F+  +I++N+TG  F VEH SILY ICW  LK   + D
Sbjct: 1426 KSISVLLRRSTFHQSHFSISFTFLAELIEKNVTGDIFAVEHASILYNICWFSLKSFRDND 1485

Query: 1388 TSKK---SIIATRTIVRGLKIAFIRTRLVPELHQKVARSLAVLY-------------ASD 1519
            T      S I    +V GLK+AFI  R VP L QKV+R LAVLY             +S 
Sbjct: 1486 TRNHDCDSFIPAPVVVSGLKLAFILCREVPMLFQKVSRLLAVLYTLPHSNKAFSMLSSSS 1545

Query: 1520 EALSKCDSPCVNQWAAYFHQASIGTYLNNHYSDRHQDHIHQEPVKVE------------- 1660
              LS+C      QWA+YFHQAS+GT+LN+      + H  Q+   V+             
Sbjct: 1546 NVLSEC------QWASYFHQASLGTHLNHQLFSSVEKHKDQKTTDVDIRLLLLTLLEVQM 1599

Query: 1661 ----KIEKDLEASVLEFFSNLPNVAILCISMLEDD-SDFLSNLLPHSP-TSVWIVLSRLN 1822
                +   DLE  VL+F   LP+V I+CISML DD +  L  LLP++P T  WI+LSR N
Sbjct: 1600 KLAPESVLDLEGFVLKFLQGLPHVTIVCISMLGDDFASLLRELLPYNPSTHAWIMLSRFN 1659

Query: 1823 SNDVPIFVMLPVASTFQDSA--------GFPINKDCSVRRPWDCPWVGDTVMDRAAPIFK 1978
            S+ +P+ ++LP+     +S+         F  NK  SV+  W CPW G T++D  AP+FK
Sbjct: 1660 SDSIPVVIVLPIDYILSESSEEGEDSSSSFLFNKKTSVKS-WHCPW-GHTIVDEVAPLFK 1717

Query: 1979 SILEEFHTT-------DPNQSVII----NKKLDQCLSELLSDIEKKWFGRWKQLLWGEWS 2125
             ILEE +T+       D  ++ ++     +KLD+CLS  L DIE  WFG WK LL GEW 
Sbjct: 1718 MILEENYTSSSAYPLEDTKKNRLLWWTQRRKLDECLSVFLRDIEDIWFGSWKHLLLGEWL 1777

Query: 2126 DYKNIVC---KLKDHLKSEY-INVDENILKVVLEGAGVNTPKEEFLSELILSNGFYVG-- 2287
            D K++     KLK  LKS+  ++V E+ILK+VL GA   + + E LS+LIL+NG YVG  
Sbjct: 1778 DTKHLDSLQKKLKKDLKSKCKVDVHESILKLVLGGARHASQRTECLSDLILNNGCYVGGI 1837

Query: 2288 -----TTNSHC----DGIDKDSFTSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLP 2440
                  +   C    D ID  S  S+S ++     +++++    R PVILV D DIQMLP
Sbjct: 1838 ECNNDRSPGECSRKQDSIDSLSL-SVSELIINAVHEIEEEDFPDREPVILVPDFDIQMLP 1896

Query: 2441 WENLPILRHQDVSRMPSIMSISSTIRKKCSYDDN---DHMVFPTIDPRDAYYVLNPQGDL 2611
            WENLP+LR+Q+V RMPS+ SIS T  + C Y +    D  VFP IDP DAYY+LNP GDL
Sbjct: 1897 WENLPVLRNQEVYRMPSVASISFTYDRCCHYQEKVGTDSAVFPMIDPLDAYYLLNPGGDL 1956

Query: 2612 KQLEHNLAEWFSKQKFE--GIVGTSPTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRN 2785
               E     WF  Q  +  G  GTSPT D+LS+ LKS D+F+Y GHGSG   I GD ++ 
Sbjct: 1957 SSTEAEFGSWFKDQNLQASGTTGTSPTVDELSVALKSHDLFMYFGHGSGVQYIPGDEIQK 2016

Query: 2786 LDRCAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLV 2965
            LDRCAA +L+GCSSGS+ LNG Y P G  L YL AGSP +V +LW++T     +  K ++
Sbjct: 2017 LDRCAATLLMGCSSGSISLNGCYTPKGAPLYYLHAGSPVIVANLWEVTDKDIDRFGKAML 2076

Query: 2966 -------------------------------------------KSLMHSKDDKTT----- 3001
                                                       KS+   KDD  T     
Sbjct: 2077 DACIRTRSITSAGCAQCSQISVELEKLKIDDDKRKGKKKTSKKKSVEPCKDDICTIGCKH 2136

Query: 3002 ---VGSSMGSARESCYFPYIQGASLICYGIKTRIKKR 3103
               +GS MG ARE+C  P++ GA+ +CYG+ T I+K+
Sbjct: 2137 RPKIGSFMGQAREACTLPFLIGAAPVCYGVPTGIRKK 2173



 Score =  250 bits (639), Expect = 6e-64
 Identities = 149/347 (42%), Positives = 207/347 (59%), Gaps = 9/347 (2%)
 Frame = +2

Query: 32   KHHESFDSVEFWKHCMENLEP-RIGFEQKLSILPTISSPDSFQHDHAIH--INIEEVKQA 202
            KH    +SVEFW  CM+  +   +GF Q L ++ T+SS  S+ HDHA    I I+EVKQ+
Sbjct: 919  KHCALSNSVEFWTSCMKRSKSLEVGFRQSLIVISTLSSSHSYIHDHATKADITIDEVKQS 978

Query: 203  ATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICH 382
            A++LI   PLS  S F+A  LYYDL E+M+ +G M+            R+KL  K F+  
Sbjct: 979  ASDLIKSVPLSNTSLFLASQLYYDLGESMIARGLMVEAISHAKEAHRLRSKLFHKKFMYS 1038

Query: 383  SKKVDKVFDDNGEVIEIK-YTYKTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFL 559
             ++ +     NGEVI+ + +  +TF M SSVA  AWS  K SS+  D I+TPWN+L+C+L
Sbjct: 1039 IEQQNDTVGANGEVIQKRRFGLETFHMHSSVATAAWSSVKGSSDFDDGILTPWNILRCYL 1098

Query: 560  ESILQIGNLID---DALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSE 730
            ES LQIG L D   +   A+ +L  GK IS  Q L IF + F  AL KLYRKQ+ W ++E
Sbjct: 1099 ESTLQIGTLQDIVGNGSEAETLLRWGKDISFFQALPIFLVAFSAALGKLYRKQQLWHLAE 1158

Query: 731  FQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFNNK--SKILKELDDADSF 904
             +L+SA  I   + S ISC  C+ VLEV++ Q F DL+  RFN+   +K+ + L +A+ F
Sbjct: 1159 KELESAKHILADNCSLISCSKCRLVLEVTVDQQFGDLFRSRFNSTTGNKLYEGLSNAEFF 1218

Query: 905  YKDVEKVLNEPIWENDISSPEKSSARKTVFCDAIGIPFALQGNEENC 1045
            Y   E+ L    W N +S+PE++SAR T+FCDA+       G   NC
Sbjct: 1219 YSSAEENLKLYEWRNCLSNPEEASARNTMFCDALLSVGKAVGISSNC 1265


>ref|XP_021988126.1| separase [Helianthus annuus]
 gb|OTG10686.1| putative separase [Helianthus annuus]
          Length = 2108

 Score =  484 bits (1245), Expect = e-142
 Identities = 318/829 (38%), Positives = 426/829 (51%), Gaps = 109/829 (13%)
 Frame = +2

Query: 944  ENDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQMLIHLKMECXX 1123
            +++    +KS     + C   G       +E NC+HC P  V KS S+  +I +K EC  
Sbjct: 1293 QSEFKGVQKSKGDHIISC---GCEVTCVSDEINCWHCVPFGVMKSASVTSVIQMKWECTR 1349

Query: 1124 XXXXXXXXXXXXXXHRARGEINDEQKV-LYSISVLFSRCLFRESHFPISPEFVTTMIDQN 1300
                            A GE      + L SISVL +R  F  S+F IS  F+  +I QN
Sbjct: 1350 RRLLVKLLIGIGKCLWAHGETQRAHDIFLESISVLVNRSTFHTSNFKISFTFLGELIKQN 1409

Query: 1301 LTGLAFGVEHTSILYKICWHFLKCISNKDTSKK----SIIATRTIVRGLKIAFIRTRLVP 1468
            + G    +EH S+LY ICW  L+   +  T  +    S I    +V GLK++F   R VP
Sbjct: 1410 VIGDMLAIEHASLLYNICWFSLRSSCDNGTRNRSCDMSFIPIPIVVSGLKLSFTVCREVP 1469

Query: 1469 ELHQKVARSLAVLYASDEAL-------SKCDSPCVNQWAAYFHQASIGTYLNNHYSDRHQ 1627
            EL QKV+R LAVLY    ++       S   S   +QWAAYFHQAS+GT++N     R  
Sbjct: 1470 ELFQKVSRLLAVLYTLAPSIETFSMLTSSSSSLLESQWAAYFHQASLGTHVNYQLFSRLG 1529

Query: 1628 DHIHQEPVK-----------------VEKIEKDLEASVLEFFSNLPNVAILCISMLEDD- 1753
                Q  +                    +   DLE  VL FF  L    I+CIS+L DD 
Sbjct: 1530 TQKDQNTMDGDGSFPSSSTLLSLHRLAPESLLDLEEFVLNFFQGLHRATIICISVLGDDY 1589

Query: 1754 SDFLSNLLPHSPTSVWIVLSRLNSNDVPIFVMLPVASTFQ-------DSAGFPINKDCSV 1912
            + +L NLL + PT  WI+LSRLN +  PI V+LP+ S  +        S+G    K  S 
Sbjct: 1590 ASWLRNLLLYEPTHSWIMLSRLNLDCAPIVVILPIVSISKGSEDDDHSSSGNLFEKKSS- 1648

Query: 1913 RRPWDCPWVGDTVMDRAAPIFKSILEEFHTTDPNQSVIIN------------KKLDQCLS 2056
             +PW CPW G TV+D  AP+F++ILEE + +      + N            +KLDQ LS
Sbjct: 1649 DKPWHCPW-GRTVVDDIAPLFRTILEESYLSASGFPTLENTNQNRLRWWDQRRKLDQWLS 1707

Query: 2057 ELLSDIEKKWFGRWKQLLWGEWSDYKN---IVCKLKDHLKSE-YINVDENILKVVLEGAG 2224
            +LL D+E  WFG WK LL G  SD+K+   +  KL+  LKS+  I+V ENI+K VL G  
Sbjct: 1708 DLLRDMEDLWFGPWKHLLLGGPSDHKHFDLLHKKLRKDLKSKCKIDVHENIIKAVLRG-- 1765

Query: 2225 VNTPKEEFLSELILSNGFYVGTTNSHCDGIDKDSFTSISSVVS----KTREQMKKKGSTS 2392
                +E+ LSELI+  G YVG      DG       S+SS+VS     T  ++  +    
Sbjct: 1766 -GRRQEDCLSELIMKKGCYVGGRELINDG-------SLSSLVSDLLLSTMHEIGIEEYVD 1817

Query: 2393 RLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISSTIRKKC---SYDDNDHMVFPT 2563
            R PVILV D DIQMLPWE+LPILR+Q+V RMPS+ SIS T  + C     D  D  +FP 
Sbjct: 1818 REPVILVPDFDIQMLPWESLPILRNQEVYRMPSVASISCTYGRCCHSQEKDGKDSSLFPM 1877

Query: 2564 IDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTSPTQDKLSMILKSRDIFVYIGH 2743
            IDP DAYY+LNP GDL   E     WF  Q  EG  GTSP+ D+LS  LK+ D+F+Y+GH
Sbjct: 1878 IDPLDAYYLLNPGGDLSSTEAEFGNWFKDQNLEGTAGTSPSVDELSTALKNHDLFIYLGH 1937

Query: 2744 GSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAGSPAVVCSLWD 2923
            GSG   I G  ++ L+RCAA +L+GCSSGSL LNGSY P G  L Y+ AGSP ++ +LWD
Sbjct: 1938 GSGVQYIPGGEIQKLERCAATLLMGCSSGSLSLNGSYTPKGAPLHYMFAGSPVIIANLWD 1997

Query: 2924 ITTSHATQITKTLV--------------------------------------KSLMHSKD 2989
            +T     +  KT++                                      K L  SK 
Sbjct: 1998 VTDKDIDRFCKTVLDGWIKARSNISVDCAQCTQLSDKFKDMSVTDGGPRKGKKKLARSKS 2057

Query: 2990 -----------DKTTVGSSMGSARESCYFPYIQGASLICYGIKTRIKKR 3103
                        +  +GS MG AR++C  P++ GA+ +CYG+ T I+++
Sbjct: 2058 VEDCEVTLGCKHRPKIGSFMGQARQACTLPFLIGAAPVCYGVPTGIRRK 2106



 Score =  232 bits (592), Expect = 3e-58
 Identities = 136/351 (38%), Positives = 209/351 (59%), Gaps = 9/351 (2%)
 Frame = +2

Query: 32   KHHESFDSVEFWKHCMENLEP-RIGFEQKLSILPTISSPDSFQHDHAI---HINIEEVKQ 199
            +H +S  S++FWK CME  +   +GF+Q  S++ T+SSP S +HD A    H+  + +K+
Sbjct: 903  RHCKSSKSMQFWKSCMEKSKSLEVGFQQCFSVISTLSSPSSCKHDLATGYAHLTTDVIKK 962

Query: 200  AATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFIC 379
             A +L +  P++  S F++ +LYYDLSE M+ KG+MI            RTKL  K FI 
Sbjct: 963  TAFDLGNSVPVTSKSLFLSSNLYYDLSEKMISKGTMIEALLYAKEAHRLRTKLFQKNFIY 1022

Query: 380  HSKKVDKVFDDNGEVIEIK-YTYKTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCF 556
              ++ + +  DNGE+++ + Y  KTF M +SVA  AWS +K SS+  DFI+TPWNVL+C+
Sbjct: 1023 TIEQHNDIIGDNGEIVQKRGYGLKTFHMQTSVATGAWSNNKGSSDF-DFILTPWNVLRCY 1081

Query: 557  LESILQIGNL---IDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMS 727
            LESI Q+G +   + +   A+++ + GK IS  Q L IF + F  AL KLY KQ+ W ++
Sbjct: 1082 LESIRQVGTIQEIVGNGSEAESLFLWGKNISSFQSLPIFLVSFSTALGKLYCKQQCWHLA 1141

Query: 728  EFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFNNKS-KILKELDDADSF 904
            E +L+SA  +   S S ISC+ CK ++E ++ + F DL   RFN+     L  L  A++F
Sbjct: 1142 EKELESANRMLVDSFSLISCLKCKLIMEATVGKQFGDLCRRRFNSTDVNKLDGLSKAENF 1201

Query: 905  YKDVEKVLNEPIWENDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQ 1057
            Y+   + L    W+N IS P+++ +  T+FCD +     + GN+     CQ
Sbjct: 1202 YRSALEKLKLSEWKNCISYPKETVSTNTMFCDRV----LVGGNDSISSDCQ 1248


>ref|XP_020997748.1| separase-like [Arachis duranensis]
          Length = 928

 Score =  419 bits (1077), Expect = e-126
 Identities = 294/935 (31%), Positives = 469/935 (50%), Gaps = 79/935 (8%)
 Frame = +2

Query: 545  LQCFLESILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRK 715
            +QC+LES LQ+G    +  DA  A+   + GK  S+   L  F + FC  L K+Y ++R 
Sbjct: 1    MQCYLESTLQVGLICKISGDAELAKAYFLDGKDTSYSLHLPSFIVAFCSELGKIYIEKRD 60

Query: 716  WIMSEFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFNNKSKILKELDDA 895
            W  +E +LQ+A  I   S + + C  CK + EV++ Q + DL+  ++    ++     + 
Sbjct: 61   WNSAEKELQNAKQILRDSNTTLCCSKCKLIFEVTLDQYYGDLHQSKYKT-CEVKSFSSEH 119

Query: 896  DSFYKDVEKVLNEPIWENDISSPEKSSARKTVFCDAIG-----IPFALQGNEENC----- 1045
             SFY+     L+ P W+N +S PE  S  KT   D I      I F +    +N      
Sbjct: 120  ISFYESALAKLDLPEWKNSVSCPEDGSL-KTAAVDVISAGTSCICFTMNEAAQNVIMEPT 178

Query: 1046 ------FHCQPLKVKKSMSLQMLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDE-QKV 1204
                  +HC   +V +   L   I+ K E                  R    + DE QK+
Sbjct: 179  KEGSWHWHCIATEVLEGRLLSDFINFKWEFVRRKLSMKLLARLV---RCFAYVGDETQKI 235

Query: 1205 LY-SISVLFSRCLFRESHFPISPEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISN 1381
            L  S ++L S+  F E++  I+ ++   ++ + + G  F VE   IL +IC ++    ++
Sbjct: 236  LLKSTTMLHSKNTFSETNSAIAIDYFQHIVPKVILGDMFAVERAEILKEICLYYWNIYNS 295

Query: 1382 KDTSKKSI------IATRTIVRGLKIAFIRTRLVPELHQKVARSLAVLYASDEALSKCDS 1543
            K T+ ++I      I+ + +   L +AF+ +R VP++  KV++ LAV Y   ++  +   
Sbjct: 296  KATTTRNIFQNESCISLKDLASWLMVAFVLSREVPDIFHKVSQLLAVTYVVSDSREQLSL 355

Query: 1544 PCV------NQWAAYFHQASIGTY------LNNHYSDRHQDHIHQEPVKVEKIEKDLEA- 1684
            P +      N WA+YFHQASIGT+      L        + +I         +  D E  
Sbjct: 356  PYISKSLGENAWASYFHQASIGTHDTHSSRLTGRCKGSDKSNIGGTYNLSRLVPDDTEVL 415

Query: 1685 --SVLEFFSNLPNVAILCISMLEDDSDFLSNLLPHSPT-SVWIVLSRLNSNDVPIFVMLP 1855
               V +F  +LP+  ++C+++L    D+ S L   SP+   WI+LSRL     PI  +LP
Sbjct: 416  AECVKQFLGDLPSTTVICLTLL--GHDYASLLKEFSPSVKAWILLSRLTFESEPIVALLP 473

Query: 1856 VASTFQDSAGFPINKDCSVRRPWDCPWVGDTVMDRAAPIFKSILEEFHT------TDPNQ 2017
            +    +D    PI     + R W CPW G T +D   P FK+IL+E ++      T   Q
Sbjct: 474  LHPISEDVIDPPILPKYKILRDWWCPW-GSTALDEVVPAFKNILKEDYSRELEENTSEQQ 532

Query: 2018 SVIIN--KKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYKNIVCKLKDHLKSEY----- 2176
             +  N  K L+Q L +LL  IE+ WFG WK LL GE  + KN    L +HL  E      
Sbjct: 533  QLWWNHRKTLNQRLRQLLRSIEESWFGSWKCLLLGELLNCKNFE-SLLEHLVKELRSECK 591

Query: 2177 INVDENILKVVLEGAG-------VNTPKEEFLSELILSNGFYVGTTNSHCDGIDKDSFTS 2335
            + V+E +++V+L G         +   K+ +++++   +    G   +  DG    S  +
Sbjct: 592  LEVNEGLVRVILGGTRHVCGREKLKCKKDCYIAKVGHCDQGMSGILLNAADGFGVSSDKA 651

Query: 2336 ISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISS-- 2509
               +     E    K S  R P+ILVLD ++QMLPWEN+PILR  +V RMPS+ SIS+  
Sbjct: 652  FQLLKHALIELDADKNS-KREPIILVLDYEVQMLPWENIPILRQLEVYRMPSVSSISALG 710

Query: 2510 -TIRKKCSYDDNDHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTSPT 2686
             T  +           FP+IDP  A+Y+LNP G + + ++    WF +   +G  G +PT
Sbjct: 711  DTGSRSQKEAGRKCPSFPSIDPLHAFYILNPDGTVPECQNEFENWFKENNLKGNTGLAPT 770

Query: 2687 QDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPPLG 2866
            +   +  L+  D+F+YIGHGSGT   S +  ++L++C A +L+GC+SG+++L G+  PL 
Sbjct: 771  RKLWASALEKHDLFIYIGHGSGTKYFSLENFQHLNKCGAVVLLGCNSGTVYLQGNCAPLC 830

Query: 2867 VVLDYLVAGSPAVVCSLWDITTSHATQITKTLVKSLMHSK-----------DDKTTVGSS 3013
            + L YL AGSPA+V +LW I+    +   K L+++ +  +           D   T+G+ 
Sbjct: 831  IPLQYLAAGSPAIVANLWKISAKDISTFGKYLLQAWVKKRFELEKECPNNCDHPPTLGAF 890

Query: 3014 MGSARESCYFPYIQGASLICYGIKTRI--KKRVIS 3112
            +G AR++C   ++ GA+ +CYG+ TRI  KKRV S
Sbjct: 891  IGRARDACEQEFMTGAAPVCYGVPTRICVKKRVSS 925


>ref|XP_021285607.1| separase isoform X4 [Herrania umbratica]
 ref|XP_021285608.1| separase isoform X4 [Herrania umbratica]
          Length = 1798

 Score =  420 bits (1079), Expect = e-120
 Identities = 281/841 (33%), Positives = 425/841 (50%), Gaps = 122/841 (14%)
 Frame = +2

Query: 947  NDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQMLIHLKMECXXX 1126
            +D  S ++S + K    D +    A       C+ C P ++ KS  L   +++K E    
Sbjct: 958  SDTCSEKESLSEKGSCRDELRNETACICKRTKCWQCLPTEIMKSGLLNCFLNMKWEYAHR 1017

Query: 1127 XXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPISPEFVTTMIDQNL 1303
                             G+I++  KV++ SISVL SR    ++       F+  +I + +
Sbjct: 1018 KLLVRVLTGIGKCLEYHGQIHELHKVVWQSISVLVSRKSITQTCSSAHDAFLLDLIGREI 1077

Query: 1304 TGLAFGVEHTSILYKICWHFLKCISNKDTS----KKSIIATRTIVRGLKIAFIRTRLVPE 1471
             G  F VE  +ILY I W  +K + +KDT       S +    IV  LKIAF+  R VP 
Sbjct: 1078 LGDVFAVERAAILYSIGWITVKNMHSKDTRIVCCDLSNVQLSKIVHWLKIAFVLCREVPV 1137

Query: 1472 LHQKVARSLAVLY---ASDE--ALSKCDSPCVNQWAAYFHQASIGTYLNNHYS------- 1615
            L QKV+R L+ +Y   A+ E  +L  C +   + WA+YFHQAS+GT+LN  +        
Sbjct: 1138 LFQKVSRLLSAIYLLSATTELFSLPSCKALSESHWASYFHQASLGTHLNYQFFPNTCGRS 1197

Query: 1616 ------DRHQDH------IHQEPVKVEKIE----KDLEASVLEFFSNLPNVAILCISMLE 1747
                  D    H      +H E   + ++     KD E  V+ F++ LP  AI+CIS+L 
Sbjct: 1198 NAQCFVDSRDSHAIGSSCLHTETSTLLRLAPESVKDFEKFVMNFYACLPCTAIICISLLG 1257

Query: 1748 DDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDSAGFPINKDCSVR-- 1915
                 L   L  +P+ +  W++LSRLNSN+ P+ ++LP+ S  ++ +   +  D + R  
Sbjct: 1258 HAYTSLLQELLLNPSCIHAWMLLSRLNSNNQPVVLLLPLDSVLEEVSDDAVPDDDNARAC 1317

Query: 1916 ----------RPWDCPWVGDTVMDRAAPIFKSILEEFHTTDPNQSV-----------IIN 2032
                      + W CPW G TV+D  AP F+ ILEE   T  N  +           +I 
Sbjct: 1318 QKLHQQMNSGKKWHCPW-GSTVVDDVAPAFRGILEENFITTSNFLIEDTKSTRSLWWVIR 1376

Query: 2033 KKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYKNIVC---KLKDHLKSEY-INVDENIL 2200
            KK+D+ L +LLS++E  W G W+ +L G+  D K++     KL   LKS+  ++++E+ L
Sbjct: 1377 KKVDRQLGKLLSNLEDSWLGPWRHVLLGDCLDCKSLNMVHKKLVRDLKSKCKMDINESFL 1436

Query: 2201 KVVLEGAGVNTPKEEFLSELILSNGFYVGTTNSH------CDGIDKDSFTSISSVVSKTR 2362
            K+VL GA  +  +E  LS+  L  G Y+G    H       +GIDK S  + S ++ K  
Sbjct: 1437 KLVLGGAKYDI-EEACLSQQCLKKGCYMGMLEHHGEEIYRSNGIDKVSALA-SQLIHKAV 1494

Query: 2363 EQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISSTIRKKCSYDDN 2542
            +++  + + +R P+ILVLD D+QMLPWE++PILR Q+V RMPS+ SIS T+ +   Y + 
Sbjct: 1495 DELHLEDTITREPIILVLDYDVQMLPWESIPILRQQEVYRMPSVGSISLTLERSWHYQEQ 1554

Query: 2543 ---DHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTSPTQDKLSMILK 2713
               +   FP IDP DA+Y+LNP GDL   +     WF  Q FEG  G  PT ++L+  LK
Sbjct: 1555 VGRNAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGIVPTAEELATALK 1614

Query: 2714 SRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAG 2893
              D+F+Y GHGSG   +S   ++ LD+CAA +L+GCSSGSL LNG Y P G+ L YL AG
Sbjct: 1615 CHDLFLYFGHGSGEQYLSRKEIQELDKCAATLLMGCSSGSLALNGCYIPRGISLSYLRAG 1674

Query: 2894 SPAVVCSLWDITTSHATQITKTLVK----------------------------------- 2968
            SP  + +LW++T     +  K ++                                    
Sbjct: 1675 SPVTIANLWEVTDKDIDRFGKAVLSAWLRERLEPADCSQCNQLVKEFEEMKIRGRSKGTC 1734

Query: 2969 ----------------SLMHSKDDKTTVGSSMGSARESCYFPYIQGASLICYGIKTRIKK 3100
                            SL ++ D +  +GS +G ARE+C  P++ GAS +CYG+ T I++
Sbjct: 1735 RKKVASSNIDETSNGDSLKNTCDHRPKIGSFVGRARETCTLPFLNGASPVCYGVPTGIRR 1794

Query: 3101 R 3103
            +
Sbjct: 1795 K 1795



 Score =  170 bits (431), Expect = 6e-39
 Identities = 109/336 (32%), Positives = 172/336 (51%), Gaps = 19/336 (5%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLSILPTISSPDSFQHDHAIH-------INIEEVK 196
            E+  S+ FW  C+    P + GF+Q L     I   ++F H    H       + +  VK
Sbjct: 541  ENSKSIVFWIGCLSGSHPGLLGFQQNL-----ICFFNTFTHGFKNHERDFQSAVTVNNVK 595

Query: 197  QAATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFI 376
            Q  + LI  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F 
Sbjct: 596  QIVSELISSDPVRSHSLFLAGYLYYDLCERHISSGQLFEGLSYAKEAFRLRSQLFKRKFA 655

Query: 377  CHSKKVDKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNV 544
               ++  + +++ G++ EI        K  ++  +VA   WS D  S + +   ++PWNV
Sbjct: 656  FSIEEQVEKYNETGDIGEIALKVINGPKDLQVQRTVASELWSFDSSSWDLRGCYLSPWNV 715

Query: 545  LQCFLESILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRK 715
            LQC+LESILQ+G    +  + + A+  L+ GK IS  Q L +F   F   L KLYRK++ 
Sbjct: 716  LQCYLESILQVGYINEMTGNGVEAETFLVWGKTISCSQSLPLFEATFSSVLGKLYRKKQL 775

Query: 716  WIMSEFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFN----NKSKILKE 883
            W  +E +LQSA  I   S S  SC+ C+ +LE+++ Q   DL+ + F+    N SK  + 
Sbjct: 776  WHFAEEELQSAKQILVGSSSDYSCIKCRLMLEINLDQQLGDLFCNLFDSTIINNSK--ER 833

Query: 884  LDDADSFYKDVEKVLNEPIWENDISSPEKSSARKTV 991
            L  A+  YK   + LN   W++ ISS E++   +T+
Sbjct: 834  LSHAEFLYKSALEKLNHSEWKS-ISSDEETDENRTI 868


>ref|XP_022942952.1| separase isoform X2 [Cucurbita moschata]
          Length = 2174

 Score =  422 bits (1084), Expect = e-120
 Identities = 273/814 (33%), Positives = 419/814 (51%), Gaps = 114/814 (14%)
 Frame = +2

Query: 1004 IGIPFALQGNEENCFHCQPLKVKKSMSLQMLIHLKMECXXXXXXXXXXXXXXXXHRARGE 1183
            +G   + +  +  C+ C  +++ ++  +   I+LK E                    RG+
Sbjct: 1361 LGCGASCKNGKVGCWQCLQMEIMEAGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRGQ 1420

Query: 1184 IND-EQKVLYSISVLFSRCLFRESHFPISPEFVTTMIDQNLTGLAFGVEHTSILYKICWH 1360
            I+   + +L S+S+L SR +F ++H  + P  +  ++ + + G  F VE  S+LY ICW 
Sbjct: 1421 IHQTHETILKSMSILVSRNIFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWF 1480

Query: 1361 FLKCISNKDTS----KKSIIATRTIVRGLKIAFIRTRLVPELHQKVARSLAVLYASDEAL 1528
             LK     DT       S + ++T+V  L +A +    VP L QKV++ LAV++    ++
Sbjct: 1481 ALKSYKYDDTKIICCPLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSI 1540

Query: 1529 SKCDSPCVNQ------WAAYFHQASIGTYLNNHYSDRH------QD-------HIHQEPV 1651
                 P  N+      WA+YFHQASIGT+LN+ +          QD          ++ V
Sbjct: 1541 ELLSLPSSNKILTDSHWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAKGFDTGEQTV 1600

Query: 1652 KVEK----IEKDLEASVLEFFSNLPNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLS 1813
            K+ +      +DLE  V +FF  LP VA++CIS++  D   L   + H P+SV  WI++S
Sbjct: 1601 KLFRRGSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVS 1660

Query: 1814 RLNSNDVPIFVMLPVASTFQDSAGF--PINKDCSVR----RPWDCPWVGDTVMDRAAPIF 1975
             LNS   P+ ++LPV +  +DS  +  P + D   R    + W CPW G +V+D  AP F
Sbjct: 1661 HLNSKRQPLVLLLPVETILKDSEDYSNPQSDDICERNDSTKHWQCPW-GSSVIDEIAPAF 1719

Query: 1976 KSILEEFHTTD---PNQSVIINK--------KLDQCLSELLSDIEKKWFGRWKQLLWGEW 2122
            ++ILE+ + +    P++    N+        KLD CL +LL  IE  W G WK +L G+W
Sbjct: 1720 RTILEDNYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDW 1779

Query: 2123 SDYKNIVCKLKD---HLKSEY-INVDENILKVVLEGAGVNTPKEEFLSELILSNGFYVGT 2290
            S+ K++   L     +LKS+  ++V+E +LK++LEG+        F S+L    G +VG 
Sbjct: 1780 SNRKHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGS--ENVLVGFDSKLYSRKGCFVGR 1837

Query: 2291 TNSH-----------CDGIDKDSFTSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQML 2437
               H            +G+DK S  ++  ++   +++++ +  TSR P+ILVLD D+QML
Sbjct: 1838 ARFHDKERSNPFQNALNGVDKLSTLALK-LIQDAKKELEGEDGTSREPIILVLDYDVQML 1896

Query: 2438 PWENLPILRHQDVSRMPSIMSISSTIRKKCSYDDNDHMV---FPTIDPRDAYYVLNPQGD 2608
            PWEN+PIL++Q+V RMPS+ SI  T+  +    + D  +   FP+IDP DA+Y+LNP GD
Sbjct: 1897 PWENIPILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGD 1956

Query: 2609 LKQLEHNLAEWFSKQKFEGIVGTSPTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNL 2788
            L   +     WF  Q  EG  G +P   +L   LKSRD+F+Y GHGSG   I    ++ L
Sbjct: 1957 LSSTQIEFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKL 2016

Query: 2789 DRCAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAGSPAVVCSLWDITTS----------- 2935
            D CAA++L+GCSSGSL LNG Y P G+ L YL AGSP +V +LW++T             
Sbjct: 2017 DACAASLLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILE 2076

Query: 2936 -----------------------HATQITKTLVKSLMHSKDDKTT--------------- 3001
                                    A +I+  L    + SK   TT               
Sbjct: 2077 AWLRERSCALPSSVQCDIVTKELEAMKISSKLANKKVASKSLPTTCESSSSKDHSVHSRM 2136

Query: 3002 VGSSMGSARESCYFPYIQGASLICYGIKTRIKKR 3103
            +GS +  ARE+C   Y+ GAS +CYG+ T I+K+
Sbjct: 2137 IGSFLCEAREACNLRYLIGASPVCYGVPTSIRKK 2170



 Score =  162 bits (411), Expect = 2e-36
 Identities = 106/319 (33%), Positives = 172/319 (53%), Gaps = 11/319 (3%)
 Frame = +2

Query: 53   SVEFWKHCMENLEPR-IGFEQKLSILPTISSPDSFQHDHA--IHINIEEVKQAATNLIDR 223
            S +FW +C++ L    +GF+Q  S L +  +  S +H+ +  +H+ ++EVK+AA  LI  
Sbjct: 948  SNDFWTNCLKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISH 1007

Query: 224  DPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICH-SKKVDK 400
             P    S F AG+LYYDL E ++ +G +             R+KL  + F     +  +K
Sbjct: 1008 IPACKSSIFSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEK 1067

Query: 401  VFDDNGEVIEIKYTYKTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFLESILQIG 580
              +      +  Y  K  R   SV++  WS DK S + +   ++P+NVLQC+LES LQ+G
Sbjct: 1068 YCEITHASQKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVG 1127

Query: 581  ---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSEFQLQSAL 751
                +I +   A+ +L  GK+IS L+ L +F + F  AL K+YRK++ W +++ +L+ A 
Sbjct: 1128 LVHEIIGNGSEAETLLQWGKSISCLRSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAK 1187

Query: 752  SIWNSSQSHISCVHCKAVLEVSIKQVFADL----YIHRFNNKSKILKELDDADSFYKDVE 919
             I   + +  SC+ C+ VLEV + Q   DL    Y++   N S+ L  L +A+  YK   
Sbjct: 1188 QILTDNIT--SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEEL--LINAEELYKLAL 1243

Query: 920  KVLNEPIWENDISSPEKSS 976
            + LN   W+N IS P++ S
Sbjct: 1244 EKLNLSGWKNSISCPDEDS 1262


>ref|XP_022748572.1| separase isoform X2 [Durio zibethinus]
          Length = 2198

 Score =  421 bits (1083), Expect = e-120
 Identities = 286/850 (33%), Positives = 417/850 (49%), Gaps = 118/850 (13%)
 Frame = +2

Query: 911  DVEKVLNEPIWENDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQ 1090
            DV   L++  WE + S   K S       D +    A       C+HC P ++ KS  L 
Sbjct: 1339 DVASKLSDTCWEKESSLLGKGSCT-----DELRNEIACICKGTKCWHCLPTEIVKSGLLN 1393

Query: 1091 MLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPIS 1267
              I++K E                      E ++  KV++ S+S L SR     +     
Sbjct: 1394 YFINMKWEYARRKLLMRVLTGIGKCLLYHDETHEIHKVVWQSLSALVSRTTLPLTFSSAH 1453

Query: 1268 PEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNKDTS----KKSIIATRTIVRGL 1435
              F+  +I + + G  F VE  +ILY I W  +K   +KD+       S +    IV  L
Sbjct: 1454 GAFLLDLIGKEILGDTFAVERAAILYCIGWITVKNFHSKDSRIVCCDLSNVQLSKIVHWL 1513

Query: 1436 KIAFIRTRLVPELHQKVARSLAVLYASDE-----ALSKCDSPCVNQWAAYFHQASIGTYL 1600
            K+AF+  R VP L QKV+R L+ +Y         AL  C +   + WA++FHQAS+GT+L
Sbjct: 1514 KLAFVLCREVPVLFQKVSRLLSAIYLLSATIEHFALPSCKALSESHWASFFHQASLGTHL 1573

Query: 1601 NNHYSDRHQDHIHQEPV-----------------------KVEKIEKDLEASVLEFFSNL 1711
            N  +        + +P                           +   +LE  V+ F   L
Sbjct: 1574 NYQFFPNTSGRSNAQPFVDSRDLHVIGSSCLDTETSTLLRLAPESVNNLEQFVMNFLVGL 1633

Query: 1712 PNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDSAG 1885
            P   I+CIS+L      L   L   P+S+  W++LSRLNS + P+ ++LP+ S  +D+A 
Sbjct: 1634 PCTTIICISLLGRAYTGLLQELLLYPSSIQAWMLLSRLNSKNQPVVLLLPLDSVLEDAAA 1693

Query: 1886 ---------FPINKDCSVRRPWDCPWVGDTVMDRAAPIFKSILEE-FHTT-----DPNQS 2020
                       + +  +  + W CPW G TV+D  AP FK ILEE F TT     +  +S
Sbjct: 1694 PDDDNARACQELRQQMNSSKKWHCPW-GSTVVDDVAPAFKVILEENFITTSGYPLEDTKS 1752

Query: 2021 V-----IINKKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYK---NIVCKLKDHLKSE- 2173
            +      I KK+DQ L +LLS++E  W G WK +L G+  D K    +  KL   LKS+ 
Sbjct: 1753 IRSLWWTIRKKVDQQLGKLLSNLEDSWLGPWKHVLLGDCLDCKILNTVHKKLVRDLKSKC 1812

Query: 2174 YINVDENILKVVLEGAGVNTPKEEFLSELILSNGFYV------GTTNSHCDGIDKDSFTS 2335
             ++++E+ LK+VL GA  N  +E  LS+  L  G Y+      G  N   +GID  S + 
Sbjct: 1813 KMDINESFLKLVLGGAKFNV-EEACLSQQCLKKGCYIGMLEHRGEENCRSNGIDNIS-SL 1870

Query: 2336 ISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISSTI 2515
             S ++S+   ++  +G+ SR P+ILVLD D+QMLPWE++PILR Q+V RMPS+ SIS T+
Sbjct: 1871 ASQLISEAVNELHAEGTISREPIILVLDSDVQMLPWESIPILRQQEVYRMPSVGSISMTL 1930

Query: 2516 RKKCSYD---DNDHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTSPT 2686
             +   Y    D     FP IDP DA+Y+LNP GDL   +     WF  Q FEG  GT PT
Sbjct: 1931 ERNWHYQEQVDGKAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGTVPT 1990

Query: 2687 QDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPPLG 2866
             ++L+  LKS D+F+Y GHGSG   +S D ++ LDRCAA +L+GCSSGSL LNG Y P G
Sbjct: 1991 TEQLATALKSHDLFLYFGHGSGEQYLSRDEIQELDRCAATLLMGCSSGSLRLNGCYIPRG 2050

Query: 2867 VVLDYLVAGSPAVVCSLWDITTSHATQITKTLVKSLMHSK-------------------- 2986
            V L YL AGSP  + +LW++T     +  K ++ + +  +                    
Sbjct: 2051 VSLSYLRAGSPVTIANLWEVTDKDIDRFGKAVLNAWLRERMDLADCSQCNQLVKEFEAMK 2110

Query: 2987 ------------------------------DDKTTVGSSMGSARESCYFPYIQGASLICY 3076
                                          D +  +GS +G ARE+C  P++ GAS +CY
Sbjct: 2111 IKGRKGNSRKKVASSNLAETTDGGSFKNACDHRPKIGSFVGQARETCTLPFLNGASPVCY 2170

Query: 3077 GIKTRIKKRV 3106
            G+ T I++++
Sbjct: 2171 GVPTGIRRKI 2180



 Score =  166 bits (421), Expect = 1e-37
 Identities = 105/325 (32%), Positives = 166/325 (51%), Gaps = 14/325 (4%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLS-ILPTISSPDSFQHDHAIHINIEEVKQAATNL 214
            ES +S++FW HC+   +P + GF+Q L+    T +   + + D    + +  VKQ  + L
Sbjct: 932  ESSESIDFWIHCLSGSQPGLLGFQQNLTCFFNTSNHGKNHERDFQSAVTVNNVKQIVSEL 991

Query: 215  IDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICHSKKV 394
            I  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F    ++ 
Sbjct: 992  IASDPVRSHSLFLAGYLYYDLCERHISNGQLFEGLSYAKEAFQLRSQLFKRKFTFSIQEQ 1051

Query: 395  DKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFLE 562
             + +++ GE+ EI        K  ++   VA   WS D  S +     ++PWNVLQ +LE
Sbjct: 1052 VEKYNETGEIGEIAVKVINGPKNLQIHRIVASELWSFDSSSWDLCGCYLSPWNVLQSYLE 1111

Query: 563  SILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSEF 733
            SILQ+G    +  + + A++ L+ GK+IS  Q L +F   F   L KLYRK+R W  +E 
Sbjct: 1112 SILQVGCINEMTGNGIEAESFLLWGKSISCSQNLPLFEAAFSSVLGKLYRKKRLWDFAEK 1171

Query: 734  QLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHR-----FNNKSKILKELDDAD 898
            +L+SA  I   S S  SC+ C+ +LEV++     DL+ +       NN +   + L  A+
Sbjct: 1172 ELKSAKRILVDSSSCYSCIKCRLMLEVNLNLQLGDLFRNLSDCVVLNNSN---ERLSHAE 1228

Query: 899  SFYKDVEKVLNEPIWENDISSPEKS 973
              YK   + LN   W+N I   E +
Sbjct: 1229 FLYKSAIEKLNHSEWKNIIFDEESN 1253


>ref|XP_021285606.1| separase isoform X3 [Herrania umbratica]
          Length = 2156

 Score =  420 bits (1079), Expect = e-119
 Identities = 281/841 (33%), Positives = 425/841 (50%), Gaps = 122/841 (14%)
 Frame = +2

Query: 947  NDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQMLIHLKMECXXX 1126
            +D  S ++S + K    D +    A       C+ C P ++ KS  L   +++K E    
Sbjct: 1316 SDTCSEKESLSEKGSCRDELRNETACICKRTKCWQCLPTEIMKSGLLNCFLNMKWEYAHR 1375

Query: 1127 XXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPISPEFVTTMIDQNL 1303
                             G+I++  KV++ SISVL SR    ++       F+  +I + +
Sbjct: 1376 KLLVRVLTGIGKCLEYHGQIHELHKVVWQSISVLVSRKSITQTCSSAHDAFLLDLIGREI 1435

Query: 1304 TGLAFGVEHTSILYKICWHFLKCISNKDTS----KKSIIATRTIVRGLKIAFIRTRLVPE 1471
             G  F VE  +ILY I W  +K + +KDT       S +    IV  LKIAF+  R VP 
Sbjct: 1436 LGDVFAVERAAILYSIGWITVKNMHSKDTRIVCCDLSNVQLSKIVHWLKIAFVLCREVPV 1495

Query: 1472 LHQKVARSLAVLY---ASDE--ALSKCDSPCVNQWAAYFHQASIGTYLNNHYS------- 1615
            L QKV+R L+ +Y   A+ E  +L  C +   + WA+YFHQAS+GT+LN  +        
Sbjct: 1496 LFQKVSRLLSAIYLLSATTELFSLPSCKALSESHWASYFHQASLGTHLNYQFFPNTCGRS 1555

Query: 1616 ------DRHQDH------IHQEPVKVEKIE----KDLEASVLEFFSNLPNVAILCISMLE 1747
                  D    H      +H E   + ++     KD E  V+ F++ LP  AI+CIS+L 
Sbjct: 1556 NAQCFVDSRDSHAIGSSCLHTETSTLLRLAPESVKDFEKFVMNFYACLPCTAIICISLLG 1615

Query: 1748 DDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDSAGFPINKDCSVR-- 1915
                 L   L  +P+ +  W++LSRLNSN+ P+ ++LP+ S  ++ +   +  D + R  
Sbjct: 1616 HAYTSLLQELLLNPSCIHAWMLLSRLNSNNQPVVLLLPLDSVLEEVSDDAVPDDDNARAC 1675

Query: 1916 ----------RPWDCPWVGDTVMDRAAPIFKSILEEFHTTDPNQSV-----------IIN 2032
                      + W CPW G TV+D  AP F+ ILEE   T  N  +           +I 
Sbjct: 1676 QKLHQQMNSGKKWHCPW-GSTVVDDVAPAFRGILEENFITTSNFLIEDTKSTRSLWWVIR 1734

Query: 2033 KKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYKNIVC---KLKDHLKSEY-INVDENIL 2200
            KK+D+ L +LLS++E  W G W+ +L G+  D K++     KL   LKS+  ++++E+ L
Sbjct: 1735 KKVDRQLGKLLSNLEDSWLGPWRHVLLGDCLDCKSLNMVHKKLVRDLKSKCKMDINESFL 1794

Query: 2201 KVVLEGAGVNTPKEEFLSELILSNGFYVGTTNSH------CDGIDKDSFTSISSVVSKTR 2362
            K+VL GA  +  +E  LS+  L  G Y+G    H       +GIDK S  + S ++ K  
Sbjct: 1795 KLVLGGAKYDI-EEACLSQQCLKKGCYMGMLEHHGEEIYRSNGIDKVSALA-SQLIHKAV 1852

Query: 2363 EQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISSTIRKKCSYDDN 2542
            +++  + + +R P+ILVLD D+QMLPWE++PILR Q+V RMPS+ SIS T+ +   Y + 
Sbjct: 1853 DELHLEDTITREPIILVLDYDVQMLPWESIPILRQQEVYRMPSVGSISLTLERSWHYQEQ 1912

Query: 2543 ---DHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTSPTQDKLSMILK 2713
               +   FP IDP DA+Y+LNP GDL   +     WF  Q FEG  G  PT ++L+  LK
Sbjct: 1913 VGRNAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGIVPTAEELATALK 1972

Query: 2714 SRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAG 2893
              D+F+Y GHGSG   +S   ++ LD+CAA +L+GCSSGSL LNG Y P G+ L YL AG
Sbjct: 1973 CHDLFLYFGHGSGEQYLSRKEIQELDKCAATLLMGCSSGSLALNGCYIPRGISLSYLRAG 2032

Query: 2894 SPAVVCSLWDITTSHATQITKTLVK----------------------------------- 2968
            SP  + +LW++T     +  K ++                                    
Sbjct: 2033 SPVTIANLWEVTDKDIDRFGKAVLSAWLRERLEPADCSQCNQLVKEFEEMKIRGRSKGTC 2092

Query: 2969 ----------------SLMHSKDDKTTVGSSMGSARESCYFPYIQGASLICYGIKTRIKK 3100
                            SL ++ D +  +GS +G ARE+C  P++ GAS +CYG+ T I++
Sbjct: 2093 RKKVASSNIDETSNGDSLKNTCDHRPKIGSFVGRARETCTLPFLNGASPVCYGVPTGIRR 2152

Query: 3101 R 3103
            +
Sbjct: 2153 K 2153



 Score =  170 bits (431), Expect = 6e-39
 Identities = 109/336 (32%), Positives = 172/336 (51%), Gaps = 19/336 (5%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLSILPTISSPDSFQHDHAIH-------INIEEVK 196
            E+  S+ FW  C+    P + GF+Q L     I   ++F H    H       + +  VK
Sbjct: 899  ENSKSIVFWIGCLSGSHPGLLGFQQNL-----ICFFNTFTHGFKNHERDFQSAVTVNNVK 953

Query: 197  QAATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFI 376
            Q  + LI  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F 
Sbjct: 954  QIVSELISSDPVRSHSLFLAGYLYYDLCERHISSGQLFEGLSYAKEAFRLRSQLFKRKFA 1013

Query: 377  CHSKKVDKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNV 544
               ++  + +++ G++ EI        K  ++  +VA   WS D  S + +   ++PWNV
Sbjct: 1014 FSIEEQVEKYNETGDIGEIALKVINGPKDLQVQRTVASELWSFDSSSWDLRGCYLSPWNV 1073

Query: 545  LQCFLESILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRK 715
            LQC+LESILQ+G    +  + + A+  L+ GK IS  Q L +F   F   L KLYRK++ 
Sbjct: 1074 LQCYLESILQVGYINEMTGNGVEAETFLVWGKTISCSQSLPLFEATFSSVLGKLYRKKQL 1133

Query: 716  WIMSEFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFN----NKSKILKE 883
            W  +E +LQSA  I   S S  SC+ C+ +LE+++ Q   DL+ + F+    N SK  + 
Sbjct: 1134 WHFAEEELQSAKQILVGSSSDYSCIKCRLMLEINLDQQLGDLFCNLFDSTIINNSK--ER 1191

Query: 884  LDDADSFYKDVEKVLNEPIWENDISSPEKSSARKTV 991
            L  A+  YK   + LN   W++ ISS E++   +T+
Sbjct: 1192 LSHAEFLYKSALEKLNHSEWKS-ISSDEETDENRTI 1226


>ref|XP_021285605.1| separase isoform X2 [Herrania umbratica]
          Length = 2198

 Score =  420 bits (1079), Expect = e-119
 Identities = 281/841 (33%), Positives = 425/841 (50%), Gaps = 122/841 (14%)
 Frame = +2

Query: 947  NDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQMLIHLKMECXXX 1126
            +D  S ++S + K    D +    A       C+ C P ++ KS  L   +++K E    
Sbjct: 1358 SDTCSEKESLSEKGSCRDELRNETACICKRTKCWQCLPTEIMKSGLLNCFLNMKWEYAHR 1417

Query: 1127 XXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPISPEFVTTMIDQNL 1303
                             G+I++  KV++ SISVL SR    ++       F+  +I + +
Sbjct: 1418 KLLVRVLTGIGKCLEYHGQIHELHKVVWQSISVLVSRKSITQTCSSAHDAFLLDLIGREI 1477

Query: 1304 TGLAFGVEHTSILYKICWHFLKCISNKDTS----KKSIIATRTIVRGLKIAFIRTRLVPE 1471
             G  F VE  +ILY I W  +K + +KDT       S +    IV  LKIAF+  R VP 
Sbjct: 1478 LGDVFAVERAAILYSIGWITVKNMHSKDTRIVCCDLSNVQLSKIVHWLKIAFVLCREVPV 1537

Query: 1472 LHQKVARSLAVLY---ASDE--ALSKCDSPCVNQWAAYFHQASIGTYLNNHYS------- 1615
            L QKV+R L+ +Y   A+ E  +L  C +   + WA+YFHQAS+GT+LN  +        
Sbjct: 1538 LFQKVSRLLSAIYLLSATTELFSLPSCKALSESHWASYFHQASLGTHLNYQFFPNTCGRS 1597

Query: 1616 ------DRHQDH------IHQEPVKVEKIE----KDLEASVLEFFSNLPNVAILCISMLE 1747
                  D    H      +H E   + ++     KD E  V+ F++ LP  AI+CIS+L 
Sbjct: 1598 NAQCFVDSRDSHAIGSSCLHTETSTLLRLAPESVKDFEKFVMNFYACLPCTAIICISLLG 1657

Query: 1748 DDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDSAGFPINKDCSVR-- 1915
                 L   L  +P+ +  W++LSRLNSN+ P+ ++LP+ S  ++ +   +  D + R  
Sbjct: 1658 HAYTSLLQELLLNPSCIHAWMLLSRLNSNNQPVVLLLPLDSVLEEVSDDAVPDDDNARAC 1717

Query: 1916 ----------RPWDCPWVGDTVMDRAAPIFKSILEEFHTTDPNQSV-----------IIN 2032
                      + W CPW G TV+D  AP F+ ILEE   T  N  +           +I 
Sbjct: 1718 QKLHQQMNSGKKWHCPW-GSTVVDDVAPAFRGILEENFITTSNFLIEDTKSTRSLWWVIR 1776

Query: 2033 KKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYKNIVC---KLKDHLKSEY-INVDENIL 2200
            KK+D+ L +LLS++E  W G W+ +L G+  D K++     KL   LKS+  ++++E+ L
Sbjct: 1777 KKVDRQLGKLLSNLEDSWLGPWRHVLLGDCLDCKSLNMVHKKLVRDLKSKCKMDINESFL 1836

Query: 2201 KVVLEGAGVNTPKEEFLSELILSNGFYVGTTNSH------CDGIDKDSFTSISSVVSKTR 2362
            K+VL GA  +  +E  LS+  L  G Y+G    H       +GIDK S  + S ++ K  
Sbjct: 1837 KLVLGGAKYDI-EEACLSQQCLKKGCYMGMLEHHGEEIYRSNGIDKVSALA-SQLIHKAV 1894

Query: 2363 EQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISSTIRKKCSYDDN 2542
            +++  + + +R P+ILVLD D+QMLPWE++PILR Q+V RMPS+ SIS T+ +   Y + 
Sbjct: 1895 DELHLEDTITREPIILVLDYDVQMLPWESIPILRQQEVYRMPSVGSISLTLERSWHYQEQ 1954

Query: 2543 ---DHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTSPTQDKLSMILK 2713
               +   FP IDP DA+Y+LNP GDL   +     WF  Q FEG  G  PT ++L+  LK
Sbjct: 1955 VGRNAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGIVPTAEELATALK 2014

Query: 2714 SRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAG 2893
              D+F+Y GHGSG   +S   ++ LD+CAA +L+GCSSGSL LNG Y P G+ L YL AG
Sbjct: 2015 CHDLFLYFGHGSGEQYLSRKEIQELDKCAATLLMGCSSGSLALNGCYIPRGISLSYLRAG 2074

Query: 2894 SPAVVCSLWDITTSHATQITKTLVK----------------------------------- 2968
            SP  + +LW++T     +  K ++                                    
Sbjct: 2075 SPVTIANLWEVTDKDIDRFGKAVLSAWLRERLEPADCSQCNQLVKEFEEMKIRGRSKGTC 2134

Query: 2969 ----------------SLMHSKDDKTTVGSSMGSARESCYFPYIQGASLICYGIKTRIKK 3100
                            SL ++ D +  +GS +G ARE+C  P++ GAS +CYG+ T I++
Sbjct: 2135 RKKVASSNIDETSNGDSLKNTCDHRPKIGSFVGRARETCTLPFLNGASPVCYGVPTGIRR 2194

Query: 3101 R 3103
            +
Sbjct: 2195 K 2195



 Score =  170 bits (431), Expect = 7e-39
 Identities = 109/336 (32%), Positives = 172/336 (51%), Gaps = 19/336 (5%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLSILPTISSPDSFQHDHAIH-------INIEEVK 196
            E+  S+ FW  C+    P + GF+Q L     I   ++F H    H       + +  VK
Sbjct: 941  ENSKSIVFWIGCLSGSHPGLLGFQQNL-----ICFFNTFTHGFKNHERDFQSAVTVNNVK 995

Query: 197  QAATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFI 376
            Q  + LI  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F 
Sbjct: 996  QIVSELISSDPVRSHSLFLAGYLYYDLCERHISSGQLFEGLSYAKEAFRLRSQLFKRKFA 1055

Query: 377  CHSKKVDKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNV 544
               ++  + +++ G++ EI        K  ++  +VA   WS D  S + +   ++PWNV
Sbjct: 1056 FSIEEQVEKYNETGDIGEIALKVINGPKDLQVQRTVASELWSFDSSSWDLRGCYLSPWNV 1115

Query: 545  LQCFLESILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRK 715
            LQC+LESILQ+G    +  + + A+  L+ GK IS  Q L +F   F   L KLYRK++ 
Sbjct: 1116 LQCYLESILQVGYINEMTGNGVEAETFLVWGKTISCSQSLPLFEATFSSVLGKLYRKKQL 1175

Query: 716  WIMSEFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFN----NKSKILKE 883
            W  +E +LQSA  I   S S  SC+ C+ +LE+++ Q   DL+ + F+    N SK  + 
Sbjct: 1176 WHFAEEELQSAKQILVGSSSDYSCIKCRLMLEINLDQQLGDLFCNLFDSTIINNSK--ER 1233

Query: 884  LDDADSFYKDVEKVLNEPIWENDISSPEKSSARKTV 991
            L  A+  YK   + LN   W++ ISS E++   +T+
Sbjct: 1234 LSHAEFLYKSALEKLNHSEWKS-ISSDEETDENRTI 1268


>ref|XP_021285604.1| separase isoform X1 [Herrania umbratica]
          Length = 2201

 Score =  420 bits (1079), Expect = e-119
 Identities = 281/841 (33%), Positives = 425/841 (50%), Gaps = 122/841 (14%)
 Frame = +2

Query: 947  NDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQMLIHLKMECXXX 1126
            +D  S ++S + K    D +    A       C+ C P ++ KS  L   +++K E    
Sbjct: 1361 SDTCSEKESLSEKGSCRDELRNETACICKRTKCWQCLPTEIMKSGLLNCFLNMKWEYAHR 1420

Query: 1127 XXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPISPEFVTTMIDQNL 1303
                             G+I++  KV++ SISVL SR    ++       F+  +I + +
Sbjct: 1421 KLLVRVLTGIGKCLEYHGQIHELHKVVWQSISVLVSRKSITQTCSSAHDAFLLDLIGREI 1480

Query: 1304 TGLAFGVEHTSILYKICWHFLKCISNKDTS----KKSIIATRTIVRGLKIAFIRTRLVPE 1471
             G  F VE  +ILY I W  +K + +KDT       S +    IV  LKIAF+  R VP 
Sbjct: 1481 LGDVFAVERAAILYSIGWITVKNMHSKDTRIVCCDLSNVQLSKIVHWLKIAFVLCREVPV 1540

Query: 1472 LHQKVARSLAVLY---ASDE--ALSKCDSPCVNQWAAYFHQASIGTYLNNHYS------- 1615
            L QKV+R L+ +Y   A+ E  +L  C +   + WA+YFHQAS+GT+LN  +        
Sbjct: 1541 LFQKVSRLLSAIYLLSATTELFSLPSCKALSESHWASYFHQASLGTHLNYQFFPNTCGRS 1600

Query: 1616 ------DRHQDH------IHQEPVKVEKIE----KDLEASVLEFFSNLPNVAILCISMLE 1747
                  D    H      +H E   + ++     KD E  V+ F++ LP  AI+CIS+L 
Sbjct: 1601 NAQCFVDSRDSHAIGSSCLHTETSTLLRLAPESVKDFEKFVMNFYACLPCTAIICISLLG 1660

Query: 1748 DDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDSAGFPINKDCSVR-- 1915
                 L   L  +P+ +  W++LSRLNSN+ P+ ++LP+ S  ++ +   +  D + R  
Sbjct: 1661 HAYTSLLQELLLNPSCIHAWMLLSRLNSNNQPVVLLLPLDSVLEEVSDDAVPDDDNARAC 1720

Query: 1916 ----------RPWDCPWVGDTVMDRAAPIFKSILEEFHTTDPNQSV-----------IIN 2032
                      + W CPW G TV+D  AP F+ ILEE   T  N  +           +I 
Sbjct: 1721 QKLHQQMNSGKKWHCPW-GSTVVDDVAPAFRGILEENFITTSNFLIEDTKSTRSLWWVIR 1779

Query: 2033 KKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYKNIVC---KLKDHLKSEY-INVDENIL 2200
            KK+D+ L +LLS++E  W G W+ +L G+  D K++     KL   LKS+  ++++E+ L
Sbjct: 1780 KKVDRQLGKLLSNLEDSWLGPWRHVLLGDCLDCKSLNMVHKKLVRDLKSKCKMDINESFL 1839

Query: 2201 KVVLEGAGVNTPKEEFLSELILSNGFYVGTTNSH------CDGIDKDSFTSISSVVSKTR 2362
            K+VL GA  +  +E  LS+  L  G Y+G    H       +GIDK S  + S ++ K  
Sbjct: 1840 KLVLGGAKYDI-EEACLSQQCLKKGCYMGMLEHHGEEIYRSNGIDKVSALA-SQLIHKAV 1897

Query: 2363 EQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISSTIRKKCSYDDN 2542
            +++  + + +R P+ILVLD D+QMLPWE++PILR Q+V RMPS+ SIS T+ +   Y + 
Sbjct: 1898 DELHLEDTITREPIILVLDYDVQMLPWESIPILRQQEVYRMPSVGSISLTLERSWHYQEQ 1957

Query: 2543 ---DHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTSPTQDKLSMILK 2713
               +   FP IDP DA+Y+LNP GDL   +     WF  Q FEG  G  PT ++L+  LK
Sbjct: 1958 VGRNAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGIVPTAEELATALK 2017

Query: 2714 SRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAG 2893
              D+F+Y GHGSG   +S   ++ LD+CAA +L+GCSSGSL LNG Y P G+ L YL AG
Sbjct: 2018 CHDLFLYFGHGSGEQYLSRKEIQELDKCAATLLMGCSSGSLALNGCYIPRGISLSYLRAG 2077

Query: 2894 SPAVVCSLWDITTSHATQITKTLVK----------------------------------- 2968
            SP  + +LW++T     +  K ++                                    
Sbjct: 2078 SPVTIANLWEVTDKDIDRFGKAVLSAWLRERLEPADCSQCNQLVKEFEEMKIRGRSKGTC 2137

Query: 2969 ----------------SLMHSKDDKTTVGSSMGSARESCYFPYIQGASLICYGIKTRIKK 3100
                            SL ++ D +  +GS +G ARE+C  P++ GAS +CYG+ T I++
Sbjct: 2138 RKKVASSNIDETSNGDSLKNTCDHRPKIGSFVGRARETCTLPFLNGASPVCYGVPTGIRR 2197

Query: 3101 R 3103
            +
Sbjct: 2198 K 2198



 Score =  170 bits (431), Expect = 7e-39
 Identities = 109/336 (32%), Positives = 172/336 (51%), Gaps = 19/336 (5%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLSILPTISSPDSFQHDHAIH-------INIEEVK 196
            E+  S+ FW  C+    P + GF+Q L     I   ++F H    H       + +  VK
Sbjct: 944  ENSKSIVFWIGCLSGSHPGLLGFQQNL-----ICFFNTFTHGFKNHERDFQSAVTVNNVK 998

Query: 197  QAATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFI 376
            Q  + LI  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F 
Sbjct: 999  QIVSELISSDPVRSHSLFLAGYLYYDLCERHISSGQLFEGLSYAKEAFRLRSQLFKRKFA 1058

Query: 377  CHSKKVDKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNV 544
               ++  + +++ G++ EI        K  ++  +VA   WS D  S + +   ++PWNV
Sbjct: 1059 FSIEEQVEKYNETGDIGEIALKVINGPKDLQVQRTVASELWSFDSSSWDLRGCYLSPWNV 1118

Query: 545  LQCFLESILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRK 715
            LQC+LESILQ+G    +  + + A+  L+ GK IS  Q L +F   F   L KLYRK++ 
Sbjct: 1119 LQCYLESILQVGYINEMTGNGVEAETFLVWGKTISCSQSLPLFEATFSSVLGKLYRKKQL 1178

Query: 716  WIMSEFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFN----NKSKILKE 883
            W  +E +LQSA  I   S S  SC+ C+ +LE+++ Q   DL+ + F+    N SK  + 
Sbjct: 1179 WHFAEEELQSAKQILVGSSSDYSCIKCRLMLEINLDQQLGDLFCNLFDSTIINNSK--ER 1236

Query: 884  LDDADSFYKDVEKVLNEPIWENDISSPEKSSARKTV 991
            L  A+  YK   + LN   W++ ISS E++   +T+
Sbjct: 1237 LSHAEFLYKSALEKLNHSEWKS-ISSDEETDENRTI 1271


>ref|XP_022942953.1| separase isoform X3 [Cucurbita moschata]
          Length = 2174

 Score =  418 bits (1074), Expect = e-119
 Identities = 273/814 (33%), Positives = 419/814 (51%), Gaps = 114/814 (14%)
 Frame = +2

Query: 1004 IGIPFALQGNEENCFHCQPLKVKKSMSLQMLIHLKMECXXXXXXXXXXXXXXXXHRARGE 1183
            +G   + +  +  C+ C  +++ ++  +   I+LK E                    RG+
Sbjct: 1361 LGCGASCKNGKVGCWQCLQMEIMEAGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRGQ 1420

Query: 1184 IND-EQKVLYSISVLFSRCLFRESHFPISPEFVTTMIDQNLTGLAFGVEHTSILYKICWH 1360
            I+   + +L S+S+L SR +F ++H  + P  +  ++ + + G  F VE  S+LY ICW 
Sbjct: 1421 IHQTHETILKSMSILVSRNIFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWF 1480

Query: 1361 FLKCISNKDTS----KKSIIATRTIVRGLKIAFIRTRLVPELHQKVARSLAVLYASDEAL 1528
             LK     DT       S + ++T+V  L +A +    VP L QKV++ LAV++    ++
Sbjct: 1481 ALKSYKYDDTKIICCPLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSI 1540

Query: 1529 SKCDSPCVNQ------WAAYFHQASIGTYLNNHYSDRH------QD-------HIHQEPV 1651
                 P  N+      WA+YFHQASIGT+LN+ +          QD          ++ V
Sbjct: 1541 ELLSLPSSNKILTDSHWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAKGFDTGEQTV 1600

Query: 1652 KVEK---IEKDLEASVLEFFSNLPNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSR 1816
            K+ +     +DLE  V +FF  LP VA++CIS++  D   L   + H P+SV  WI++S 
Sbjct: 1601 KLFRGSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSH 1660

Query: 1817 LNSNDVPIFVMLPVASTF-QDSAGF--PINKDCSVR----RPWDCPWVGDTVMDRAAPIF 1975
            LNS   P+ ++LPV +   +DS  +  P + D   R    + W CPW G +V+D  AP F
Sbjct: 1661 LNSKRQPLVLLLPVETILKEDSEDYSNPQSDDICERNDSTKHWQCPW-GSSVIDEIAPAF 1719

Query: 1976 KSILEEFHTTD---PNQSVIINK--------KLDQCLSELLSDIEKKWFGRWKQLLWGEW 2122
            ++ILE+ + +    P++    N+        KLD CL +LL  IE  W G WK +L G+W
Sbjct: 1720 RTILEDNYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDW 1779

Query: 2123 SDYKNIVCKLKD---HLKSEY-INVDENILKVVLEGAGVNTPKEEFLSELILSNGFYVGT 2290
            S+ K++   L     +LKS+  ++V+E +LK++LEG+        F S+L    G +VG 
Sbjct: 1780 SNRKHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGS--ENVLVGFDSKLYSRKGCFVGR 1837

Query: 2291 TNSH-----------CDGIDKDSFTSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQML 2437
               H            +G+DK S  ++  ++   +++++ +  TSR P+ILVLD D+QML
Sbjct: 1838 ARFHDKERSNPFQNALNGVDKLSTLALK-LIQDAKKELEGEDGTSREPIILVLDYDVQML 1896

Query: 2438 PWENLPILRHQDVSRMPSIMSISSTIRKKCSYDDNDHMV---FPTIDPRDAYYVLNPQGD 2608
            PWEN+PIL++Q+V RMPS+ SI  T+  +    + D  +   FP+IDP DA+Y+LNP GD
Sbjct: 1897 PWENIPILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGD 1956

Query: 2609 LKQLEHNLAEWFSKQKFEGIVGTSPTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNL 2788
            L   +     WF  Q  EG  G +P   +L   LKSRD+F+Y GHGSG   I    ++ L
Sbjct: 1957 LSSTQIEFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKL 2016

Query: 2789 DRCAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAGSPAVVCSLWDITTS----------- 2935
            D CAA++L+GCSSGSL LNG Y P G+ L YL AGSP +V +LW++T             
Sbjct: 2017 DACAASLLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILE 2076

Query: 2936 -----------------------HATQITKTLVKSLMHSKDDKTT--------------- 3001
                                    A +I+  L    + SK   TT               
Sbjct: 2077 AWLRERSCALPSSVQCDIVTKELEAMKISSKLANKKVASKSLPTTCESSSSKDHSVHSRM 2136

Query: 3002 VGSSMGSARESCYFPYIQGASLICYGIKTRIKKR 3103
            +GS +  ARE+C   Y+ GAS +CYG+ T I+K+
Sbjct: 2137 IGSFLCEAREACNLRYLIGASPVCYGVPTSIRKK 2170



 Score =  162 bits (411), Expect = 2e-36
 Identities = 106/319 (33%), Positives = 172/319 (53%), Gaps = 11/319 (3%)
 Frame = +2

Query: 53   SVEFWKHCMENLEPR-IGFEQKLSILPTISSPDSFQHDHA--IHINIEEVKQAATNLIDR 223
            S +FW +C++ L    +GF+Q  S L +  +  S +H+ +  +H+ ++EVK+AA  LI  
Sbjct: 948  SNDFWTNCLKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISH 1007

Query: 224  DPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICH-SKKVDK 400
             P    S F AG+LYYDL E ++ +G +             R+KL  + F     +  +K
Sbjct: 1008 IPACKSSIFSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEK 1067

Query: 401  VFDDNGEVIEIKYTYKTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFLESILQIG 580
              +      +  Y  K  R   SV++  WS DK S + +   ++P+NVLQC+LES LQ+G
Sbjct: 1068 YCEITHASQKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVG 1127

Query: 581  ---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSEFQLQSAL 751
                +I +   A+ +L  GK+IS L+ L +F + F  AL K+YRK++ W +++ +L+ A 
Sbjct: 1128 LVHEIIGNGSEAETLLQWGKSISCLRSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAK 1187

Query: 752  SIWNSSQSHISCVHCKAVLEVSIKQVFADL----YIHRFNNKSKILKELDDADSFYKDVE 919
             I   + +  SC+ C+ VLEV + Q   DL    Y++   N S+ L  L +A+  YK   
Sbjct: 1188 QILTDNIT--SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEEL--LINAEELYKLAL 1243

Query: 920  KVLNEPIWENDISSPEKSS 976
            + LN   W+N IS P++ S
Sbjct: 1244 EKLNLSGWKNSISCPDEDS 1262


>gb|KJB11859.1| hypothetical protein B456_002G207900 [Gossypium raimondii]
          Length = 1807

 Score =  416 bits (1069), Expect = e-119
 Identities = 284/852 (33%), Positives = 423/852 (49%), Gaps = 121/852 (14%)
 Frame = +2

Query: 911  DVEKVLNEPIWENDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQ 1090
            +V   L++  WE D S   K S  + +  +      A   N+  C+HC P ++ KS  L 
Sbjct: 961  NVASKLSDTCWEKDSSLLGKGSCMEELTSE-----IACFCNQTKCWHCLPAEIMKSGLLI 1015

Query: 1091 MLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPIS 1267
              I++K E                      + ++  +V++ SISVL SR  + ++     
Sbjct: 1016 YFINMKWEYAHRKLLVRILTGTGKCLGYGDQTHEIHEVVWQSISVLVSRNAYTQTCSSAH 1075

Query: 1268 PEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNK----DTSKKSIIATRTIVRGL 1435
              F+  +I +   G  F +E  +ILY+I W  LK   +K    D    S +     V  L
Sbjct: 1076 RTFLLDLIGRETVGDTFAIERAAILYRIGWMTLKGFHSKVARIDCCDLSKVQLSKTVHWL 1135

Query: 1436 KIAFIRTRLVPELHQKVARSLAVLY---ASDE--ALSKCDSPCVNQWAAYFHQASIGTYL 1600
            K+AF+ +R VP L QKV+R L+ +Y   A+ E  AL  C     + WA+YFHQAS+GT+L
Sbjct: 1136 KLAFVLSREVPVLFQKVSRLLSAIYLLSATSEHFALPSCKELSESHWASYFHQASLGTHL 1195

Query: 1601 NNHY---------SDRHQDH----------IHQEPVKVEKIE----KDLEASVLEFFSNL 1711
            NN +         + R  D            H E   + ++      DLE  V+ F+  L
Sbjct: 1196 NNQFFPSTSGRSNAQRFVDSGDLNGVVSSCAHTETSTLLRLAPGSVNDLEQFVMNFYVGL 1255

Query: 1712 PNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDSAG 1885
            P  AI+CIS+L  D   L   L   P+S+  W++LSRLNS + P+ ++LP+ S  ++ + 
Sbjct: 1256 PGTAIICISLLGHDYTNLLQELLLYPSSIHAWLLLSRLNSKNQPVVLLLPLDSVLEEVSD 1315

Query: 1886 FPINKDCSVR------------RPWDCPWVGDTVMDRAAPIFKSILEE---------FHT 2002
                 D + R            + W CPW G TV+D  AP FK ILEE            
Sbjct: 1316 DAAPNDDNARACQELRQLMNSGKKWHCPW-GSTVVDNVAPAFKMILEENFMTSSGCPLED 1374

Query: 2003 TDPNQSV--IINKKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYKNIVC---KLKDHLK 2167
            T   +S+  ++ KK+D  L +LLS++E  W G W+ +L G+  D +++     KL   LK
Sbjct: 1375 TKSTRSLWWMVRKKVDHQLGKLLSNLEDSWLGPWRHVLLGDCLDCRSLNTVHKKLVQDLK 1434

Query: 2168 SE-YINVDENILKVVLEGAGVNTPKEEFLSELILSNGFYVG------TTNSHCDGIDKDS 2326
            S+  ++++E+ LK+VL GA     +E  LS   L  G Y G        NS  +GID D 
Sbjct: 1435 SKCKMDINESYLKLVL-GAAKFDIEEACLSLRCLRKGCYTGKLEHHEQENSQTNGID-DV 1492

Query: 2327 FTSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSIS 2506
                S ++ +   ++  + +  R P+ILVLDL++QMLPWE++PILR Q+V RMPS+ SIS
Sbjct: 1493 SALASQLIREAVNELHMEDAICREPIILVLDLEVQMLPWESIPILRQQEVYRMPSVGSIS 1552

Query: 2507 STIRKKCSYDD---NDHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGT 2677
              + +   Y +    +   FP IDP DA+Y+LNP GDL   +     WF  Q FEG  GT
Sbjct: 1553 IILERSQRYHELACTNAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGT 1612

Query: 2678 SPTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYP 2857
             PT ++L+  LKS D+++Y GHGSG   +S D ++ L++CAA +L+GCSSGSL LNG Y 
Sbjct: 1613 VPTAEELATALKSHDLYLYFGHGSGEQYLSKDEIQGLEKCAATVLMGCSSGSLRLNGCYV 1672

Query: 2858 PLGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLVKSLMHSKDD--------------- 2992
            P GV L Y+ AGSP  + +LW++T     +  K ++ + +  + D               
Sbjct: 1673 PRGVSLSYIQAGSPVTIANLWEVTDKDIDRFGKAVLNAWLRERMDLVDCSQCNQLVKEFE 1732

Query: 2993 -----------------------------------KTTVGSSMGSARESCYFPYIQGASL 3067
                                               + TVGS +G ARESC  P++ GAS 
Sbjct: 1733 AMKIKGRKGNSRKKSASSNLTETANSGSSTNACEHRPTVGSFVGRARESCTLPFLNGASP 1792

Query: 3068 ICYGIKTRIKKR 3103
            +CYG+ T I K+
Sbjct: 1793 VCYGVPTGIMKK 1804



 Score =  145 bits (365), Expect = 4e-31
 Identities = 88/248 (35%), Positives = 134/248 (54%), Gaps = 11/248 (4%)
 Frame = +2

Query: 116  LSILPTISSPDSFQHDHAIHINIEEVKQAATNLID----RDPLSIHSWFVAGHLYYDLSE 283
            L +  +I  PDS   D    +      Q   N++D    +DP+  HS F+AG+LYYDL E
Sbjct: 623  LDLWLSIFIPDSCFVDDEFKMVSGNTLQLLYNVLDLLSVKDPVRSHSLFLAGYLYYDLCE 682

Query: 284  TMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICHSKKVDKVFDDNGEVIEIKYTY----KT 451
              + +G +             R++L  + F    + + + ++++GE+ EI  T     KT
Sbjct: 683  RQICRGRLFEGLSYAKEAFRLRSQLFKRKFTFSIEALVEKYNESGEIGEIAQTAINGPKT 742

Query: 452  FRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFLESILQIG---NLIDDALSAQNILI 622
             ++   VA   WS D  SSN     ++PWNVLQC++ESILQ+G    +I + + A++ L+
Sbjct: 743  LQVHRIVASELWSFDSSSSNLCGCYLSPWNVLQCYIESILQVGCINEMIGNGVEAESFLL 802

Query: 623  RGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSEFQLQSALSIWNSSQSHISCVHCKA 802
             GK+IS  Q L +F   F   L KLYRK+R W  +E +LQSA  I   S SH SC+ C+ 
Sbjct: 803  WGKSISCSQNLPLFEATFSSILGKLYRKKRLWNFAEQELQSAKRILVDSSSHCSCIRCRL 862

Query: 803  VLEVSIKQ 826
            +LE ++ Q
Sbjct: 863  MLEANLDQ 870


>gb|POE83410.1| separase [Quercus suber]
          Length = 1735

 Score =  415 bits (1067), Expect = e-119
 Identities = 278/811 (34%), Positives = 404/811 (49%), Gaps = 124/811 (15%)
 Frame = +2

Query: 1043 CFHCQPLKVKKSMSLQMLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKVLY-SIS 1219
            C+HC  ++V KS  +   +++K E                    RG+I++  KVL+ SIS
Sbjct: 927  CWHCLLMEVMKSGLVSNFLNMKWELVRRRLSLRLLTGIGKCFGDRGQIHETHKVLFQSIS 986

Query: 1220 VLFSRCLFRESHFPISPEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNKDTSKK 1399
            VL SR  F      + P F+   I + ++  AF +E  +IL+ I W  LK   +KDT   
Sbjct: 987  VLVSRNSFGYVDSSVPPTFLLDFIGKEISRDAFTIERAAILFNISWFSLKSYHSKDTRTS 1046

Query: 1400 SI----IATRTIVRGLKIAFIRTRLVPELHQKVARSLAVLY---ASDEALS---KCDSPC 1549
                  I    +V  L +AF+    VP L QKV++ LA +Y   AS E       C +  
Sbjct: 1047 CCDLFDIHLPKLVSWLMLAFVLCSEVPILFQKVSKLLATIYVLSASTEFFPLSPSCKALS 1106

Query: 1550 VNQWAAYFHQASIGTYLN-------------NHYSDRHQDHIH----QEPVKVEKIE--- 1669
             N WA+YFHQAS+GT+LN              H  D    H+     +E   + ++    
Sbjct: 1107 ENHWASYFHQASLGTHLNYQFFSNMIGRCKVQHLVDAEGSHVTGCGVEETSNLIRLAPDS 1166

Query: 1670 -KDLEASVLEFFSNLPNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPI 1840
             KDLE  V +FF  LP + ++CIS+L      L   L   PT V  W+++SRLNS   P+
Sbjct: 1167 IKDLEEFVAKFFVGLPCMTVICISLLGGAYASLVKDLLCYPTCVHAWMLVSRLNSKRQPV 1226

Query: 1841 FVMLPVASTFQDSAGFPIN-------KDCSVRRPWDCPWVGDTVMDRAAPIFKSILEE-- 1993
             V+LPV    +D++    N       K   +   W CPW G TV+D  AP+FK ILEE  
Sbjct: 1227 VVLLPVDPVIEDASDDDANSGSDKLYKSMDLGEHWHCPW-GSTVVDDVAPVFKLILEENY 1285

Query: 1994 -------FHTTDPNQSV--IINKKLDQCLSELLSDIEKKWFGRWKQLLWGEWSD------ 2128
                      T  N+++  +  KKLD  L +LL +IE  W G WK LL GEWS+      
Sbjct: 1286 LSSSMFPLEDTKRNRTLWWMWRKKLDCRLGKLLRNIEDSWLGPWKYLLLGEWSNRKRLDI 1345

Query: 2129 -YKNIVCKLKDHLKSEYINVDENILKVVLEGAGVNTPKEEFLSELILSNGFYVG------ 2287
             YK +V  LK   K   ++V+E++LKV+L G+      E  +S+  L +G YVG      
Sbjct: 1346 VYKKLVHDLKSKCK---LDVNESLLKVILGGSKYAFEGEACISQQCLRSGCYVGRVGYCN 1402

Query: 2288 -----TTNSHCDGIDKDSFTSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENL 2452
                 T ++  +G+DK S  ++  ++     +++ + S +R P+ILVLD ++QMLPWENL
Sbjct: 1403 EAKFGTLSNASNGVDKQSELALQ-LIQDAANELEGEDSMNREPIILVLDCEVQMLPWENL 1461

Query: 2453 PILRHQDVSRMPSIMSISSTIRKKCSYDDNDHMV---FPTIDPRDAYYVLNPQGDLKQLE 2623
            P+LR+Q+V RMPS+ SIS T+ +     +    +   FP IDP DA+Y+LNP GDL   +
Sbjct: 1462 PVLRNQEVYRMPSVGSISETLDRSHHRQEQVGRICATFPLIDPVDAFYLLNPSGDLSSTQ 1521

Query: 2624 HNLAEWFSKQKFEGIVGTSPTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAA 2803
                 WF     EG  G++PT ++L   LKS D+F+Y GHGSG   I    ++ L+ CAA
Sbjct: 1522 VEFENWFRDHNLEGKAGSAPTAEELVAALKSHDLFIYFGHGSGAQYIPRHEIQKLENCAA 1581

Query: 2804 AMLIGCSSGSLHLNGSYPPLGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLVKSLMHS 2983
             +L+GCSSGSL LNGSY P G  L YL+AGSP +V +LW++T     +  K ++ + +  
Sbjct: 1582 TLLMGCSSGSLTLNGSYVPQGTPLSYLLAGSPVIVANLWEVTDKDIDRFGKAMLDAWLRE 1641

Query: 2984 K---------------------------------------------------DDKTTVGS 3010
            +                                                   D    +GS
Sbjct: 1642 RSSPSLGCAQCDLVVEEFEAMTIRGCKGNVRRKNRRKKSPEAHDTSSFKVSCDHGPKIGS 1701

Query: 3011 SMGSARESCYFPYIQGASLICYGIKTRIKKR 3103
             M  ARE+C  P++ GAS +CYGI T I+++
Sbjct: 1702 FMSQAREACTLPFLIGASPVCYGIPTGIRRK 1732



 Score =  174 bits (440), Expect = 5e-40
 Identities = 104/319 (32%), Positives = 173/319 (54%), Gaps = 10/319 (3%)
 Frame = +2

Query: 53   SVEFWKHCMENLEPR-IGFEQKLSI-LPTISSPDSFQHDHAIHINIEEVKQAATNLIDRD 226
            SV+FW  C++   P  +GF+Q  S    +  S  + +      I ++EV++AA  LI   
Sbjct: 473  SVDFWIRCLKGSHPLLVGFQQSFSFSFVSSQSSSNLESTFRSDITVDEVREAAFQLISSV 532

Query: 227  PLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICHSKKVDKVF 406
            P+   S F+AG+LYYDL E ++  G +I            RT+L  + F+   ++  + +
Sbjct: 533  PVPSQSVFLAGYLYYDLCERLVSHGRLIEALLYAKEAHQLRTRLFQEKFMYSVEQHTEKY 592

Query: 407  DDNGEVIE-IKYTYKTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFLESILQIG- 580
            ++ G+VI+ + Y  K  ++  S+A   WS D    + +   ++PWNVLQC+LES LQIG 
Sbjct: 593  NETGDVIQKLTYGLKNLQIRRSIATEIWSFDTIQWDLEGCYLSPWNVLQCYLESTLQIGI 652

Query: 581  --NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSEFQLQSALS 754
               +  +   A+  LI GK IS  QGL +F + F   L KLYRK++ W ++E +LQSA  
Sbjct: 653  IHEITGNGAEAETFLIWGKNISCSQGLPLFIVAFSSVLGKLYRKKQLWDLAEKELQSAKK 712

Query: 755  IWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFNNKSKI----LKELDDADSFYKDVEK 922
            +   + + +SC+ C+ ++E ++     DL   R N+ S      ++ L  A++ YK    
Sbjct: 713  VLMDTSTTLSCIKCRLMMEATLDHHLGDL--SRSNSDSTTADTSVERLSHAENQYKLALD 770

Query: 923  VLNEPIWENDISSPEKSSA 979
             L+ P+W+N +S PE ++A
Sbjct: 771  KLDLPVWKNCVSCPEMANA 789


>ref|XP_022748575.1| separase isoform X4 [Durio zibethinus]
          Length = 2114

 Score =  417 bits (1073), Expect = e-119
 Identities = 287/852 (33%), Positives = 417/852 (48%), Gaps = 120/852 (14%)
 Frame = +2

Query: 911  DVEKVLNEPIWENDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQ 1090
            DV   L++  WE + S   K S       D +    A       C+HC P ++ KS  L 
Sbjct: 1253 DVASKLSDTCWEKESSLLGKGSCT-----DELRNEIACICKGTKCWHCLPTEIVKSGLLN 1307

Query: 1091 MLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPIS 1267
              I++K E                      E ++  KV++ S+S L SR     +     
Sbjct: 1308 YFINMKWEYARRKLLMRVLTGIGKCLLYHDETHEIHKVVWQSLSALVSRTTLPLTFSSAH 1367

Query: 1268 PEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNKDTS----KKSIIATRTIVRGL 1435
              F+  +I + + G  F VE  +ILY I W  +K   +KD+       S +    IV  L
Sbjct: 1368 GAFLLDLIGKEILGDTFAVERAAILYCIGWITVKNFHSKDSRIVCCDLSNVQLSKIVHWL 1427

Query: 1436 KIAFIRTRLVPELHQKVARSLAVLYASDE-----ALSKCDSPCVNQWAAYFHQASIGTYL 1600
            K+AF+  R VP L QKV+R L+ +Y         AL  C +   + WA++FHQAS+GT+L
Sbjct: 1428 KLAFVLCREVPVLFQKVSRLLSAIYLLSATIEHFALPSCKALSESHWASFFHQASLGTHL 1487

Query: 1601 NNHYSDRHQDHIHQEPV-----------------------KVEKIEKDLEASVLEFFSNL 1711
            N  +        + +P                           +   +LE  V+ F   L
Sbjct: 1488 NYQFFPNTSGRSNAQPFVDSRDLHVIGSSCLDTETSTLLRLAPESVNNLEQFVMNFLVGL 1547

Query: 1712 PNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDS-A 1882
            P   I+CIS+L      L   L   P+S+  W++LSRLNS + P+ ++LP+ S  + S A
Sbjct: 1548 PCTTIICISLLGRAYTGLLQELLLYPSSIQAWMLLSRLNSKNQPVVLLLPLDSVLEVSDA 1607

Query: 1883 GFP----------INKDCSVRRPWDCPWVGDTVMDRAAPIFKSILEE-FHTT-----DPN 2014
              P          + +  +  + W CPW G TV+D  AP FK ILEE F TT     +  
Sbjct: 1608 AAPDDDNARACQELRQQMNSSKKWHCPW-GSTVVDDVAPAFKVILEENFITTSGYPLEDT 1666

Query: 2015 QSV-----IINKKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYK---NIVCKLKDHLKS 2170
            +S+      I KK+DQ L +LLS++E  W G WK +L G+  D K    +  KL   LKS
Sbjct: 1667 KSIRSLWWTIRKKVDQQLGKLLSNLEDSWLGPWKHVLLGDCLDCKILNTVHKKLVRDLKS 1726

Query: 2171 E-YINVDENILKVVLEGAGVNTPKEEFLSELILSNGFYV------GTTNSHCDGIDKDSF 2329
            +  ++++E+ LK+VL GA  N  +E  LS+  L  G Y+      G  N   +GID  S 
Sbjct: 1727 KCKMDINESFLKLVLGGAKFNV-EEACLSQQCLKKGCYIGMLEHRGEENCRSNGIDNIS- 1784

Query: 2330 TSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISS 2509
            +  S ++S+   ++  +G+ SR P+ILVLD D+QMLPWE++PILR Q+V RMPS+ SIS 
Sbjct: 1785 SLASQLISEAVNELHAEGTISREPIILVLDSDVQMLPWESIPILRQQEVYRMPSVGSISM 1844

Query: 2510 TIRKKCSYD---DNDHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTS 2680
            T+ +   Y    D     FP IDP DA+Y+LNP GDL   +     WF  Q FEG  GT 
Sbjct: 1845 TLERNWHYQEQVDGKAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGTV 1904

Query: 2681 PTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPP 2860
            PT ++L+  LKS D+F+Y GHGSG   +S D ++ LDRCAA +L+GCSSGSL LNG Y P
Sbjct: 1905 PTTEQLATALKSHDLFLYFGHGSGEQYLSRDEIQELDRCAATLLMGCSSGSLRLNGCYIP 1964

Query: 2861 LGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLVKSLMHSK------------------ 2986
             GV L YL AGSP  + +LW++T     +  K ++ + +  +                  
Sbjct: 1965 RGVSLSYLRAGSPVTIANLWEVTDKDIDRFGKAVLNAWLRERMDLADCSQCNQLVKEFEA 2024

Query: 2987 --------------------------------DDKTTVGSSMGSARESCYFPYIQGASLI 3070
                                            D +  +GS +G ARE+C  P++ GAS +
Sbjct: 2025 MKIKGRKGNSRKKVASSNLAETTDGGSFKNACDHRPKIGSFVGQARETCTLPFLNGASPV 2084

Query: 3071 CYGIKTRIKKRV 3106
            CYG+ T I++++
Sbjct: 2085 CYGVPTGIRRKI 2096



 Score =  166 bits (421), Expect = 1e-37
 Identities = 105/325 (32%), Positives = 166/325 (51%), Gaps = 14/325 (4%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLS-ILPTISSPDSFQHDHAIHINIEEVKQAATNL 214
            ES +S++FW HC+   +P + GF+Q L+    T +   + + D    + +  VKQ  + L
Sbjct: 846  ESSESIDFWIHCLSGSQPGLLGFQQNLTCFFNTSNHGKNHERDFQSAVTVNNVKQIVSEL 905

Query: 215  IDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICHSKKV 394
            I  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F    ++ 
Sbjct: 906  IASDPVRSHSLFLAGYLYYDLCERHISNGQLFEGLSYAKEAFQLRSQLFKRKFTFSIQEQ 965

Query: 395  DKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFLE 562
             + +++ GE+ EI        K  ++   VA   WS D  S +     ++PWNVLQ +LE
Sbjct: 966  VEKYNETGEIGEIAVKVINGPKNLQIHRIVASELWSFDSSSWDLCGCYLSPWNVLQSYLE 1025

Query: 563  SILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSEF 733
            SILQ+G    +  + + A++ L+ GK+IS  Q L +F   F   L KLYRK+R W  +E 
Sbjct: 1026 SILQVGCINEMTGNGIEAESFLLWGKSISCSQNLPLFEAAFSSVLGKLYRKKRLWDFAEK 1085

Query: 734  QLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHR-----FNNKSKILKELDDAD 898
            +L+SA  I   S S  SC+ C+ +LEV++     DL+ +       NN +   + L  A+
Sbjct: 1086 ELKSAKRILVDSSSCYSCIKCRLMLEVNLNLQLGDLFRNLSDCVVLNNSN---ERLSHAE 1142

Query: 899  SFYKDVEKVLNEPIWENDISSPEKS 973
              YK   + LN   W+N I   E +
Sbjct: 1143 FLYKSAIEKLNHSEWKNIIFDEESN 1167


>ref|XP_022748574.1| separase isoform X3 [Durio zibethinus]
          Length = 2151

 Score =  417 bits (1073), Expect = e-119
 Identities = 287/852 (33%), Positives = 417/852 (48%), Gaps = 120/852 (14%)
 Frame = +2

Query: 911  DVEKVLNEPIWENDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQ 1090
            DV   L++  WE + S   K S       D +    A       C+HC P ++ KS  L 
Sbjct: 1290 DVASKLSDTCWEKESSLLGKGSCT-----DELRNEIACICKGTKCWHCLPTEIVKSGLLN 1344

Query: 1091 MLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPIS 1267
              I++K E                      E ++  KV++ S+S L SR     +     
Sbjct: 1345 YFINMKWEYARRKLLMRVLTGIGKCLLYHDETHEIHKVVWQSLSALVSRTTLPLTFSSAH 1404

Query: 1268 PEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNKDTS----KKSIIATRTIVRGL 1435
              F+  +I + + G  F VE  +ILY I W  +K   +KD+       S +    IV  L
Sbjct: 1405 GAFLLDLIGKEILGDTFAVERAAILYCIGWITVKNFHSKDSRIVCCDLSNVQLSKIVHWL 1464

Query: 1436 KIAFIRTRLVPELHQKVARSLAVLYASDE-----ALSKCDSPCVNQWAAYFHQASIGTYL 1600
            K+AF+  R VP L QKV+R L+ +Y         AL  C +   + WA++FHQAS+GT+L
Sbjct: 1465 KLAFVLCREVPVLFQKVSRLLSAIYLLSATIEHFALPSCKALSESHWASFFHQASLGTHL 1524

Query: 1601 NNHYSDRHQDHIHQEPV-----------------------KVEKIEKDLEASVLEFFSNL 1711
            N  +        + +P                           +   +LE  V+ F   L
Sbjct: 1525 NYQFFPNTSGRSNAQPFVDSRDLHVIGSSCLDTETSTLLRLAPESVNNLEQFVMNFLVGL 1584

Query: 1712 PNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDS-A 1882
            P   I+CIS+L      L   L   P+S+  W++LSRLNS + P+ ++LP+ S  + S A
Sbjct: 1585 PCTTIICISLLGRAYTGLLQELLLYPSSIQAWMLLSRLNSKNQPVVLLLPLDSVLEVSDA 1644

Query: 1883 GFP----------INKDCSVRRPWDCPWVGDTVMDRAAPIFKSILEE-FHTT-----DPN 2014
              P          + +  +  + W CPW G TV+D  AP FK ILEE F TT     +  
Sbjct: 1645 AAPDDDNARACQELRQQMNSSKKWHCPW-GSTVVDDVAPAFKVILEENFITTSGYPLEDT 1703

Query: 2015 QSV-----IINKKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYK---NIVCKLKDHLKS 2170
            +S+      I KK+DQ L +LLS++E  W G WK +L G+  D K    +  KL   LKS
Sbjct: 1704 KSIRSLWWTIRKKVDQQLGKLLSNLEDSWLGPWKHVLLGDCLDCKILNTVHKKLVRDLKS 1763

Query: 2171 E-YINVDENILKVVLEGAGVNTPKEEFLSELILSNGFYV------GTTNSHCDGIDKDSF 2329
            +  ++++E+ LK+VL GA  N  +E  LS+  L  G Y+      G  N   +GID  S 
Sbjct: 1764 KCKMDINESFLKLVLGGAKFNV-EEACLSQQCLKKGCYIGMLEHRGEENCRSNGIDNIS- 1821

Query: 2330 TSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISS 2509
            +  S ++S+   ++  +G+ SR P+ILVLD D+QMLPWE++PILR Q+V RMPS+ SIS 
Sbjct: 1822 SLASQLISEAVNELHAEGTISREPIILVLDSDVQMLPWESIPILRQQEVYRMPSVGSISM 1881

Query: 2510 TIRKKCSYD---DNDHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTS 2680
            T+ +   Y    D     FP IDP DA+Y+LNP GDL   +     WF  Q FEG  GT 
Sbjct: 1882 TLERNWHYQEQVDGKAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGTV 1941

Query: 2681 PTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPP 2860
            PT ++L+  LKS D+F+Y GHGSG   +S D ++ LDRCAA +L+GCSSGSL LNG Y P
Sbjct: 1942 PTTEQLATALKSHDLFLYFGHGSGEQYLSRDEIQELDRCAATLLMGCSSGSLRLNGCYIP 2001

Query: 2861 LGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLVKSLMHSK------------------ 2986
             GV L YL AGSP  + +LW++T     +  K ++ + +  +                  
Sbjct: 2002 RGVSLSYLRAGSPVTIANLWEVTDKDIDRFGKAVLNAWLRERMDLADCSQCNQLVKEFEA 2061

Query: 2987 --------------------------------DDKTTVGSSMGSARESCYFPYIQGASLI 3070
                                            D +  +GS +G ARE+C  P++ GAS +
Sbjct: 2062 MKIKGRKGNSRKKVASSNLAETTDGGSFKNACDHRPKIGSFVGQARETCTLPFLNGASPV 2121

Query: 3071 CYGIKTRIKKRV 3106
            CYG+ T I++++
Sbjct: 2122 CYGVPTGIRRKI 2133



 Score =  166 bits (421), Expect = 1e-37
 Identities = 105/325 (32%), Positives = 166/325 (51%), Gaps = 14/325 (4%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLS-ILPTISSPDSFQHDHAIHINIEEVKQAATNL 214
            ES +S++FW HC+   +P + GF+Q L+    T +   + + D    + +  VKQ  + L
Sbjct: 883  ESSESIDFWIHCLSGSQPGLLGFQQNLTCFFNTSNHGKNHERDFQSAVTVNNVKQIVSEL 942

Query: 215  IDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICHSKKV 394
            I  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F    ++ 
Sbjct: 943  IASDPVRSHSLFLAGYLYYDLCERHISNGQLFEGLSYAKEAFQLRSQLFKRKFTFSIQEQ 1002

Query: 395  DKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFLE 562
             + +++ GE+ EI        K  ++   VA   WS D  S +     ++PWNVLQ +LE
Sbjct: 1003 VEKYNETGEIGEIAVKVINGPKNLQIHRIVASELWSFDSSSWDLCGCYLSPWNVLQSYLE 1062

Query: 563  SILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSEF 733
            SILQ+G    +  + + A++ L+ GK+IS  Q L +F   F   L KLYRK+R W  +E 
Sbjct: 1063 SILQVGCINEMTGNGIEAESFLLWGKSISCSQNLPLFEAAFSSVLGKLYRKKRLWDFAEK 1122

Query: 734  QLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHR-----FNNKSKILKELDDAD 898
            +L+SA  I   S S  SC+ C+ +LEV++     DL+ +       NN +   + L  A+
Sbjct: 1123 ELKSAKRILVDSSSCYSCIKCRLMLEVNLNLQLGDLFRNLSDCVVLNNSN---ERLSHAE 1179

Query: 899  SFYKDVEKVLNEPIWENDISSPEKS 973
              YK   + LN   W+N I   E +
Sbjct: 1180 FLYKSAIEKLNHSEWKNIIFDEESN 1204


>ref|XP_022748571.1| separase isoform X1 [Durio zibethinus]
          Length = 2200

 Score =  417 bits (1073), Expect = e-119
 Identities = 287/852 (33%), Positives = 417/852 (48%), Gaps = 120/852 (14%)
 Frame = +2

Query: 911  DVEKVLNEPIWENDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQ 1090
            DV   L++  WE + S   K S       D +    A       C+HC P ++ KS  L 
Sbjct: 1339 DVASKLSDTCWEKESSLLGKGSCT-----DELRNEIACICKGTKCWHCLPTEIVKSGLLN 1393

Query: 1091 MLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPIS 1267
              I++K E                      E ++  KV++ S+S L SR     +     
Sbjct: 1394 YFINMKWEYARRKLLMRVLTGIGKCLLYHDETHEIHKVVWQSLSALVSRTTLPLTFSSAH 1453

Query: 1268 PEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNKDTS----KKSIIATRTIVRGL 1435
              F+  +I + + G  F VE  +ILY I W  +K   +KD+       S +    IV  L
Sbjct: 1454 GAFLLDLIGKEILGDTFAVERAAILYCIGWITVKNFHSKDSRIVCCDLSNVQLSKIVHWL 1513

Query: 1436 KIAFIRTRLVPELHQKVARSLAVLYASDE-----ALSKCDSPCVNQWAAYFHQASIGTYL 1600
            K+AF+  R VP L QKV+R L+ +Y         AL  C +   + WA++FHQAS+GT+L
Sbjct: 1514 KLAFVLCREVPVLFQKVSRLLSAIYLLSATIEHFALPSCKALSESHWASFFHQASLGTHL 1573

Query: 1601 NNHYSDRHQDHIHQEPV-----------------------KVEKIEKDLEASVLEFFSNL 1711
            N  +        + +P                           +   +LE  V+ F   L
Sbjct: 1574 NYQFFPNTSGRSNAQPFVDSRDLHVIGSSCLDTETSTLLRLAPESVNNLEQFVMNFLVGL 1633

Query: 1712 PNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDS-A 1882
            P   I+CIS+L      L   L   P+S+  W++LSRLNS + P+ ++LP+ S  + S A
Sbjct: 1634 PCTTIICISLLGRAYTGLLQELLLYPSSIQAWMLLSRLNSKNQPVVLLLPLDSVLEVSDA 1693

Query: 1883 GFP----------INKDCSVRRPWDCPWVGDTVMDRAAPIFKSILEE-FHTT-----DPN 2014
              P          + +  +  + W CPW G TV+D  AP FK ILEE F TT     +  
Sbjct: 1694 AAPDDDNARACQELRQQMNSSKKWHCPW-GSTVVDDVAPAFKVILEENFITTSGYPLEDT 1752

Query: 2015 QSV-----IINKKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYK---NIVCKLKDHLKS 2170
            +S+      I KK+DQ L +LLS++E  W G WK +L G+  D K    +  KL   LKS
Sbjct: 1753 KSIRSLWWTIRKKVDQQLGKLLSNLEDSWLGPWKHVLLGDCLDCKILNTVHKKLVRDLKS 1812

Query: 2171 E-YINVDENILKVVLEGAGVNTPKEEFLSELILSNGFYV------GTTNSHCDGIDKDSF 2329
            +  ++++E+ LK+VL GA  N  +E  LS+  L  G Y+      G  N   +GID  S 
Sbjct: 1813 KCKMDINESFLKLVLGGAKFNV-EEACLSQQCLKKGCYIGMLEHRGEENCRSNGIDNIS- 1870

Query: 2330 TSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISS 2509
            +  S ++S+   ++  +G+ SR P+ILVLD D+QMLPWE++PILR Q+V RMPS+ SIS 
Sbjct: 1871 SLASQLISEAVNELHAEGTISREPIILVLDSDVQMLPWESIPILRQQEVYRMPSVGSISM 1930

Query: 2510 TIRKKCSYD---DNDHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTS 2680
            T+ +   Y    D     FP IDP DA+Y+LNP GDL   +     WF  Q FEG  GT 
Sbjct: 1931 TLERNWHYQEQVDGKAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGTV 1990

Query: 2681 PTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPP 2860
            PT ++L+  LKS D+F+Y GHGSG   +S D ++ LDRCAA +L+GCSSGSL LNG Y P
Sbjct: 1991 PTTEQLATALKSHDLFLYFGHGSGEQYLSRDEIQELDRCAATLLMGCSSGSLRLNGCYIP 2050

Query: 2861 LGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLVKSLMHSK------------------ 2986
             GV L YL AGSP  + +LW++T     +  K ++ + +  +                  
Sbjct: 2051 RGVSLSYLRAGSPVTIANLWEVTDKDIDRFGKAVLNAWLRERMDLADCSQCNQLVKEFEA 2110

Query: 2987 --------------------------------DDKTTVGSSMGSARESCYFPYIQGASLI 3070
                                            D +  +GS +G ARE+C  P++ GAS +
Sbjct: 2111 MKIKGRKGNSRKKVASSNLAETTDGGSFKNACDHRPKIGSFVGQARETCTLPFLNGASPV 2170

Query: 3071 CYGIKTRIKKRV 3106
            CYG+ T I++++
Sbjct: 2171 CYGVPTGIRRKI 2182



 Score =  166 bits (421), Expect = 1e-37
 Identities = 105/325 (32%), Positives = 166/325 (51%), Gaps = 14/325 (4%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLS-ILPTISSPDSFQHDHAIHINIEEVKQAATNL 214
            ES +S++FW HC+   +P + GF+Q L+    T +   + + D    + +  VKQ  + L
Sbjct: 932  ESSESIDFWIHCLSGSQPGLLGFQQNLTCFFNTSNHGKNHERDFQSAVTVNNVKQIVSEL 991

Query: 215  IDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFICHSKKV 394
            I  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F    ++ 
Sbjct: 992  IASDPVRSHSLFLAGYLYYDLCERHISNGQLFEGLSYAKEAFQLRSQLFKRKFTFSIQEQ 1051

Query: 395  DKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVLQCFLE 562
             + +++ GE+ EI        K  ++   VA   WS D  S +     ++PWNVLQ +LE
Sbjct: 1052 VEKYNETGEIGEIAVKVINGPKNLQIHRIVASELWSFDSSSWDLCGCYLSPWNVLQSYLE 1111

Query: 563  SILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKWIMSEF 733
            SILQ+G    +  + + A++ L+ GK+IS  Q L +F   F   L KLYRK+R W  +E 
Sbjct: 1112 SILQVGCINEMTGNGIEAESFLLWGKSISCSQNLPLFEAAFSSVLGKLYRKKRLWDFAEK 1171

Query: 734  QLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHR-----FNNKSKILKELDDAD 898
            +L+SA  I   S S  SC+ C+ +LEV++     DL+ +       NN +   + L  A+
Sbjct: 1172 ELKSAKRILVDSSSCYSCIKCRLMLEVNLNLQLGDLFRNLSDCVVLNNSN---ERLSHAE 1228

Query: 899  SFYKDVEKVLNEPIWENDISSPEKS 973
              YK   + LN   W+N I   E +
Sbjct: 1229 FLYKSAIEKLNHSEWKNIIFDEESN 1253


>ref|XP_016711479.1| PREDICTED: separase-like [Gossypium hirsutum]
          Length = 2183

 Score =  416 bits (1070), Expect = e-118
 Identities = 284/852 (33%), Positives = 423/852 (49%), Gaps = 121/852 (14%)
 Frame = +2

Query: 911  DVEKVLNEPIWENDISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQ 1090
            +V   L++  WE D S   K S  + +  +      A   N+  C+HC P +V KS  L 
Sbjct: 1337 NVASKLSDTCWEKDSSLLGKGSCMEELTSE-----IACFCNQTKCWHCLPAEVMKSGLLI 1391

Query: 1091 MLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPIS 1267
              I++K E                        ++  +V++ SISVL SR  + ++     
Sbjct: 1392 YFINMKWEYAHRKLLVRILTGTGKCLGYGDPTHEIHEVVWQSISVLVSRNAYTQTCSSSH 1451

Query: 1268 PEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNK----DTSKKSIIATRTIVRGL 1435
              F+  +I +   G  F +E  +ILY+I W  LK   +K    D    S +     V  L
Sbjct: 1452 RTFLLDLIGRETVGDTFAIERAAILYRIGWMTLKGFHSKVARIDCCDLSKVQLSKTVHWL 1511

Query: 1436 KIAFIRTRLVPELHQKVARSLAVLY---ASDE--ALSKCDSPCVNQWAAYFHQASIGTYL 1600
            K+AF+ +R VP L QKV+R L+ +Y   A+ E  AL  C +   + WA+YFHQAS+GT+L
Sbjct: 1512 KLAFVLSREVPVLFQKVSRLLSAIYLLSATSEHFALPSCKALSESHWASYFHQASLGTHL 1571

Query: 1601 NNHY---------SDRHQDH----------IHQEPVKVEKIE----KDLEASVLEFFSNL 1711
            NN +         + R  D            H E   + ++      DLE  V+ F+  L
Sbjct: 1572 NNQFFPSTSGRLNAQRFVDSGDLNGVVSSCAHTETSTLLRLAPESVNDLEQFVMNFYVGL 1631

Query: 1712 PNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDSAG 1885
            P  AI+CIS+L  D   L   L   P+S+  W++LSRLNS + P+ ++LP+ S  ++ + 
Sbjct: 1632 PATAIICISLLGHDYTNLLQELLLDPSSIHAWMLLSRLNSKNQPVVLLLPLDSVLEEVSD 1691

Query: 1886 FPINKDCSVR------------RPWDCPWVGDTVMDRAAPIFKSILEE---------FHT 2002
                 D + R            + W CPW G  V+D  AP FK ILEE            
Sbjct: 1692 DAAPNDDNARACQELRQLMNSGKKWHCPW-GSAVVDNVAPAFKMILEENFMTSSGCPLED 1750

Query: 2003 TDPNQSV--IINKKLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYKNIVC---KLKDHLK 2167
            T   +S+  ++ KK+D  L +LLS++E  W G W+ +L G+  D +++     KL   LK
Sbjct: 1751 TKSTRSLWWMVRKKVDHQLGKLLSNLEDSWLGPWRHVLLGDCLDCRSLNTVHKKLVQDLK 1810

Query: 2168 SE-YINVDENILKVVLEGAGVNTPKEEFLSELILSNGFYVG------TTNSHCDGIDKDS 2326
            S+  +N++E+ LK+VL GA     +E  LS+  L  G Y G        NS  +GID D 
Sbjct: 1811 SKCKMNINESYLKLVL-GAAKFDIEEACLSQRCLRKGCYTGKLEHHEQENSQSNGID-DV 1868

Query: 2327 FTSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSIS 2506
                S ++ +   ++  + +  R P+ILVLDLD+QMLPWE++PILR Q+V RMPS+ SIS
Sbjct: 1869 SALASQLIREAVNELHMEDAICREPIILVLDLDVQMLPWESIPILRQQEVYRMPSVGSIS 1928

Query: 2507 STIRKKCSYDD---NDHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGT 2677
              + +   Y +    +   FP IDP DA+Y+LNP GDL   +     WF  Q FEG  GT
Sbjct: 1929 FILERSQQYHELACTNAAAFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGT 1988

Query: 2678 SPTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYP 2857
             PT ++L+  LK+ D+++Y GHGSG   +S D ++ L++CAA +L+GCSSGSL LNG Y 
Sbjct: 1989 VPTAEELATALKNHDLYLYFGHGSGEQYLSKDEIQGLEKCAATVLMGCSSGSLRLNGCYV 2048

Query: 2858 PLGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLVKSLMHSKDD--------------- 2992
            P GV L Y+ AGSP  + +LW++T     +  K ++ + +  + D               
Sbjct: 2049 PRGVSLSYIQAGSPVTIANLWEVTDKDIDRFGKAVLNAWLRERMDLVDCSQCNQLVKEFE 2108

Query: 2993 -----------------------------------KTTVGSSMGSARESCYFPYIQGASL 3067
                                               + TVGS +G ARESC  P++ GAS 
Sbjct: 2109 AMKIKGRKGNSRKKSASSNLTETANSGSSTNACEHRPTVGSFVGRARESCTLPFLNGASP 2168

Query: 3068 ICYGIKTRIKKR 3103
            +CYG+ T I ++
Sbjct: 2169 VCYGVPTGIMRK 2180



 Score =  167 bits (424), Expect = 4e-38
 Identities = 109/327 (33%), Positives = 170/327 (51%), Gaps = 17/327 (5%)
 Frame = +2

Query: 44   SFDSVEFWKHCMENLEPRI-GFEQKLSILPTISSPDSFQH---DHAIH----INIEEVKQ 199
            S  SV+FW H +   +P + GF+Q L+        ++F H   +H  H    ++   V  
Sbjct: 930  SSKSVDFWMHSLSGSQPGLLGFQQNLTCFF-----NNFNHGLKNHESHTQSAVSANNVNL 984

Query: 200  AATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFIC 379
             A+ LI  DP+  HS F+AG+LYYDL E  + +G +             R++L  + F  
Sbjct: 985  VASELIASDPVRSHSLFLAGYLYYDLCERQICRGRLFEGLSYAKEAFRLRSQLFKRKFTF 1044

Query: 380  HSKKVDKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNVL 547
              + + + ++++G++ EI  T     K  ++   VA   WS D  SSN     ++PWNVL
Sbjct: 1045 SIEALVEKYNESGDIGEIAQTAINGPKNLQVHRIVASELWSFDSNSSNLCGCYLSPWNVL 1104

Query: 548  QCFLESILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRKW 718
            +C+LESILQ+G    +I + + A++ L+ GK+IS  Q L +F   F   L KLYRK+R W
Sbjct: 1105 ECYLESILQVGCINEMIGNGVEAESFLLWGKSISCSQNLPLFEATFSSILGKLYRKKRLW 1164

Query: 719  IMSEFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFNNK--SKILKELDD 892
              +E +LQSA  I   S SH SC+ C  +LE ++ Q   DL+ + F+    +    +L  
Sbjct: 1165 NFAEQELQSAKRILVDSSSHCSCIRCLLMLEANLDQQLGDLFRNLFDISIINDSTGKLSR 1224

Query: 893  ADSFYKDVEKVLNEPIWENDISSPEKS 973
            A+  YK   + LN   W+N I   E +
Sbjct: 1225 AEVLYKSALEKLNCSEWKNIICDAESN 1251


>gb|EOX97396.1| Separase, putative [Theobroma cacao]
          Length = 2198

 Score =  416 bits (1070), Expect = e-118
 Identities = 284/840 (33%), Positives = 420/840 (50%), Gaps = 122/840 (14%)
 Frame = +2

Query: 950  DISSPEKSSARKTVFCDAIGIPFALQGNEENCFHCQPLKVKKSMSLQMLIHLKMECXXXX 1129
            D  S ++S  +K    D +    A       C+ C P ++ KS  L   I++K E     
Sbjct: 1355 DTCSEKESLFKKGSCRDELRNETACICKRTKCWQCLPTEIMKSGLLNYFINMKWEYAHRK 1414

Query: 1130 XXXXXXXXXXXXHRARGEINDEQKVLY-SISVLFSRCLFRESHFPISPEFVTTMIDQNLT 1306
                            G+ ++  KV++ SISVL SR    ++       F+  +I + + 
Sbjct: 1415 LLVRVLTGIGKCLEYHGQTHELHKVVWQSISVLVSRKRITQTCSSAHDTFLLDLIGREIL 1474

Query: 1307 GLAFGVEHTSILYKICWHFLKCISNKDTSKK----SIIATRTIVRGLKIAFIRTRLVPEL 1474
            G  F VE  +ILY I W  +K I +KDT       S +     V  LK+AF+  R VP L
Sbjct: 1475 GDVFAVERAAILYSIGWITVKNIHSKDTRAVCCDLSNVQLSKTVHWLKLAFVLCREVPVL 1534

Query: 1475 HQKVARSLAVLY---ASDE--ALSKCDSPCVNQWAAYFHQASIGTYLNNHYS-------- 1615
             QKV+R L+ +Y   A+ E  +L  C +   + WA+YFHQAS+GT+LN  +         
Sbjct: 1535 FQKVSRLLSAIYLLSATTELFSLPSCKALSESHWASYFHQASLGTHLNYQFFPNTCGRPN 1594

Query: 1616 -----DRHQDH------IHQEPVKVEKIE----KDLEASVLEFFSNLPNVAILCISMLED 1750
                 D    H      +H E   + ++     KDLE  V+ F++ LP  AI+CIS+L  
Sbjct: 1595 AQCFVDSRDSHAIGSSCLHTETSTLLRLAPESVKDLEQFVMNFYACLPCTAIICISLLGH 1654

Query: 1751 DSDFLSNLLPHSPTSV--WIVLSRLNSNDVPIFVMLPVASTFQDSAGFPINKDCSVR--- 1915
                L   L  +P+ +  W++LSRLNSN+ P+ ++LP+ S  ++ +      D + R   
Sbjct: 1655 AYTSLLQELLLNPSCIHAWMLLSRLNSNNQPVVLLLPLDSVLEEVSDDAAPDDDNARACQ 1714

Query: 1916 ---------RPWDCPWVGDTVMDRAAPIFKSILEEFHTTDPNQSV-----------IINK 2035
                     + W CPW G TV+D  AP FK ILEE   T  N  +           +I K
Sbjct: 1715 NLRQHMNSGKKWHCPW-GSTVVDDVAPAFKGILEENFITTSNFLIEDTKSTRSLWWMIRK 1773

Query: 2036 KLDQCLSELLSDIEKKWFGRWKQLLWGEWSDYKNIVC---KLKDHLKSEY-INVDENILK 2203
            K+DQ L +LLS++E  W G W+ +L G+  D K++     KL   LKS+  ++++E+ LK
Sbjct: 1774 KVDQQLGKLLSNLEDSWLGPWRHVLLGDCLDCKSLNMVHKKLVRDLKSKCKMDINESFLK 1833

Query: 2204 VVLEGAGVNTPKEEFLSELILSNGFYVGTTNS------HCDGIDKDSFTSISSVVSKTRE 2365
            +VL GA  +  +E   S   L  G Y+G            +GIDK S  + S ++ +   
Sbjct: 1834 LVLGGAKYDI-EEACFSWQCLKEGCYIGRLEHPGEEICRSNGIDKVSALA-SQLIHEAVN 1891

Query: 2366 QMKKKGSTSRLPVILVLDLDIQMLPWENLPILRHQDVSRMPSIMSISSTIRKKCSYDDN- 2542
            ++    + SR P+ILVLD D+QMLPWE++PILR Q+V RMPS+ SIS T+ +   Y +  
Sbjct: 1892 ELHLADTISREPIILVLDYDVQMLPWESIPILRQQEVYRMPSVGSISLTLERSWHYQEQV 1951

Query: 2543 --DHMVFPTIDPRDAYYVLNPQGDLKQLEHNLAEWFSKQKFEGIVGTSPTQDKLSMILKS 2716
              +  VFP IDP DA+Y+LNP GDL   +     WF  Q FEG  GT PT ++L+  LKS
Sbjct: 1952 GRNAAVFPLIDPLDAFYLLNPSGDLSSTQAEFENWFRDQNFEGKAGTVPTAEELATALKS 2011

Query: 2717 RDIFVYIGHGSGTDCISGDIVRNLDRCAAAMLIGCSSGSLHLNGSYPPLGVVLDYLVAGS 2896
             D+F+Y GHGSG   +S   ++ LD+CAA +L+GCSSGSL LNG Y P G+ L YL AGS
Sbjct: 2012 HDLFLYFGHGSGEQYLSRKEIQELDKCAATLLMGCSSGSLVLNGCYMPRGISLSYLRAGS 2071

Query: 2897 PAVVCSLWDITTSHATQITKTLVK------------------------------------ 2968
            P  + +LW++T     +  K ++                                     
Sbjct: 2072 PVTIANLWEVTDKDIDRFGKAVLSAWLSERLEPADCSQCDQLVKEFEAMKIRGRSKGTSR 2131

Query: 2969 ---------------SLMHSKDDKTTVGSSMGSARESCYFPYIQGASLICYGIKTRIKKR 3103
                           SL ++ D +  +GS +G ARE+C  P++ GAS +CYG+ T I+++
Sbjct: 2132 KKVASSNIDETSNGDSLKNTCDHRPKIGSFVGRARETCTLPFLNGASPVCYGVPTGIRRK 2191



 Score =  165 bits (418), Expect = 2e-37
 Identities = 108/336 (32%), Positives = 171/336 (50%), Gaps = 19/336 (5%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLSILPTISSPDSFQHDHAIH-------INIEEVK 196
            E+  S++FW  C+   +P + GF+Q L     I   ++F H    H       + +  VK
Sbjct: 937  ETSKSIDFWIGCLSGSQPGLLGFQQNL-----ICFFNTFTHRFKTHERDFKSAVPVNNVK 991

Query: 197  QAATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFI 376
            Q  + LI  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F 
Sbjct: 992  QIVSELISSDPVRSHSLFLAGYLYYDLCERCVSSGQLFEGLSYAKEAFQLRSQLFKRKFA 1051

Query: 377  CHSKKVDKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNV 544
               ++  +  ++ G++ EI        K  ++  +VA   WS D  S +     ++PWNV
Sbjct: 1052 FSIEEQVEKCNETGDIGEIALKVINGPKDLQVQRTVASELWSFDSSSWDLCGCYLSPWNV 1111

Query: 545  LQCFLESILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRK 715
            LQC+LESILQ+G    +  + + A+  L+ GK+IS  Q L +F   F   L KLYRK++ 
Sbjct: 1112 LQCYLESILQVGYINEMTGNGVEAETFLVWGKSISCSQSLPLFEATFSSVLGKLYRKKQL 1171

Query: 716  WIMSEFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFN----NKSKILKE 883
            W  +E +LQSA  I   S S+ SC+ C+ +LE+++ Q   DL+ + F+    N SK  + 
Sbjct: 1172 WHFAEKELQSAKQILVGSSSYYSCIKCRLMLEINLDQQLGDLFCNLFDSTIINNSK--ER 1229

Query: 884  LDDADSFYKDVEKVLNEPIWENDISSPEKSSARKTV 991
            L  A+  YK   + LN   W+  IS  E++    T+
Sbjct: 1230 LSHAEFLYKSALEKLNHSEWKR-ISFDEENDENITI 1264


>ref|XP_017971181.1| PREDICTED: separase isoform X2 [Theobroma cacao]
          Length = 2202

 Score =  416 bits (1070), Expect = e-118
 Identities = 278/809 (34%), Positives = 411/809 (50%), Gaps = 122/809 (15%)
 Frame = +2

Query: 1043 CFHCQPLKVKKSMSLQMLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKVLY-SIS 1219
            C+ C P ++ KS  L   I++K E                     G+ ++  KV++ SIS
Sbjct: 1390 CWQCLPTEIMKSGLLNYFINMKWEYAHRKLLVRVLTGIGKCLEYHGQTHELHKVVWQSIS 1449

Query: 1220 VLFSRCLFRESHFPISPEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNKDTSKK 1399
            VL SR    ++       F+  +I + + G  F VE  +ILY I W  +K I +KDT   
Sbjct: 1450 VLVSRKRITQTCSSAHDTFLLDLIGREILGDVFAVERAAILYSIGWITVKNIHSKDTRAV 1509

Query: 1400 ----SIIATRTIVRGLKIAFIRTRLVPELHQKVARSLAVLY---ASDE--ALSKCDSPCV 1552
                S +     V  LK+AF+  R VP L QKV+R L+ +Y   A+ E  +L  C +   
Sbjct: 1510 CCDLSNVQLSKTVHWLKLAFVLCREVPVLFQKVSRLLSAIYLLSATTELFSLPSCKALSE 1569

Query: 1553 NQWAAYFHQASIGTYLNNHYS-------------DRHQDH------IHQEPVKVEKIE-- 1669
            + WA+YFHQAS+GT+LN  +              D    H      +H E   + ++   
Sbjct: 1570 SHWASYFHQASLGTHLNYQFFPNTCGRPNAQCFVDSRDSHAIGSSCLHTETSTLLRLAPE 1629

Query: 1670 --KDLEASVLEFFSNLPNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSRLNSNDVP 1837
              KDLE  V++F++ LP  AI+CIS+L      L   L  +P+ +  W++LSRLNSN+ P
Sbjct: 1630 SVKDLEQFVMDFYACLPCTAIICISLLGHAYTSLLQELLLNPSCIHAWMLLSRLNSNNQP 1689

Query: 1838 IFVMLPVASTFQDSAGFPINKDCSVR------------RPWDCPWVGDTVMDRAAPIFKS 1981
            + ++LP+ S  ++ +      D + R            + W CPW G TV+D  AP FK 
Sbjct: 1690 VVLLLPLDSVLEEVSDDAAPDDDNARACQNLRQHMNSGKKWHCPW-GSTVVDDVAPAFKG 1748

Query: 1982 ILEEFHTTDPNQSV-----------IINKKLDQCLSELLSDIEKKWFGRWKQLLWGEWSD 2128
            ILEE   T  N  +           +I KK+DQ L +LLS++E  W G W+ +L G+  D
Sbjct: 1749 ILEENFITTSNFLIEDTKSTRSLWWMIRKKVDQQLGKLLSNLEDSWLGPWRHVLLGDCLD 1808

Query: 2129 YKNIVC---KLKDHLKSEY-INVDENILKVVLEGAGVNTPKEEFLSELILSNGFYVGTTN 2296
             K++     KL   LKS+  ++++E+ LK+VL GA  +  +E   S   L  G Y+G   
Sbjct: 1809 CKSLNMVHKKLVRDLKSKCKMDINESFLKLVLGGAKYDI-EEACFSWQCLKEGCYIGRLE 1867

Query: 2297 S------HCDGIDKDSFTSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENLPI 2458
                     +GIDK S  + S ++ +   ++    + SR P+ILVLD D+QMLPWE++PI
Sbjct: 1868 HPGEEICRSNGIDKVSALA-SQLIHEAVNELHLADTISREPIILVLDYDVQMLPWESIPI 1926

Query: 2459 LRHQDVSRMPSIMSISSTIRKKCSYDDN---DHMVFPTIDPRDAYYVLNPQGDLKQLEHN 2629
            LR Q+V RMPS+ SIS T+ +   Y +    +  VFP IDP DA+Y+LNP GDL   +  
Sbjct: 1927 LRQQEVYRMPSVGSISLTLERSWHYQEQVGRNAAVFPLIDPLDAFYLLNPSGDLSSTQAE 1986

Query: 2630 LAEWFSKQKFEGIVGTSPTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAAAM 2809
               WF  Q FEG  GT PT ++L+  LKS D+F+Y GHGSG   +S   ++ LD+CAA +
Sbjct: 1987 FENWFRDQNFEGKAGTVPTAEELATALKSHDLFLYFGHGSGEQYLSRKEIQELDKCAATL 2046

Query: 2810 LIGCSSGSLHLNGSYPPLGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLVK------- 2968
            L+GCSSGSL LNG Y P G+ L YL AGSP  + +LW++T     +  K ++        
Sbjct: 2047 LMGCSSGSLVLNGCYMPRGISLSYLRAGSPVTIANLWEVTDKDIDRFGKAVLSAWLSERL 2106

Query: 2969 --------------------------------------------SLMHSKDDKTTVGSSM 3016
                                                        SL ++ D +  +GS +
Sbjct: 2107 EPADCSQCDQLVKEFEAMKIRGRSKGTSRKKVASSNIDETSNGDSLKNTCDHRPKIGSFV 2166

Query: 3017 GSARESCYFPYIQGASLICYGIKTRIKKR 3103
            G ARE+C  P++ GAS +CYG+ T I+++
Sbjct: 2167 GRARETCTLPFLNGASPVCYGVPTGIRRK 2195



 Score =  167 bits (424), Expect = 4e-38
 Identities = 109/336 (32%), Positives = 172/336 (51%), Gaps = 19/336 (5%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLSILPTISSPDSFQHDHAIH-------INIEEVK 196
            E+  S++FW  C+   +P + GF+Q L     I   ++F H    H       + +  VK
Sbjct: 941  ETSKSIDFWIGCLSGSQPGLLGFQQNL-----ICFFNTFTHRFKTHERDFKSAVPVNNVK 995

Query: 197  QAATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFI 376
            Q  + LI  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F 
Sbjct: 996  QIVSELISSDPVRSHSLFLAGYLYYDLCERCVSSGQLFEGLSYAKEAFQLRSQLFKRKFA 1055

Query: 377  CHSKKVDKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNV 544
               ++  +  ++ G++ EI        K  ++  +VA   WS D  S +     ++PWNV
Sbjct: 1056 FSIEEQVEKCNETGDIGEIALKVINGPKDLQVQRTVASELWSFDSSSWDLCGCYLSPWNV 1115

Query: 545  LQCFLESILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRK 715
            LQC+LESILQ+G    +  + + A+  L+ GK+IS  Q L +F   F   L KLYRK++ 
Sbjct: 1116 LQCYLESILQVGYINEMTGNGVEAETFLVWGKSISCSQSLPLFEATFSSVLGKLYRKKQL 1175

Query: 716  WIMSEFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFN----NKSKILKE 883
            W  +E +LQSA  I   S S+ SC+ C+ +LE+++ Q   DL+ + F+    N SK  + 
Sbjct: 1176 WHFAEKELQSAKQILVGSSSYYSCIKCRLMLEINLDQQLGDLFCNLFDSTIINNSK--ER 1233

Query: 884  LDDADSFYKDVEKVLNEPIWENDISSPEKSSARKTV 991
            L  A+  YK   + LN   W+  ISS E++    T+
Sbjct: 1234 LSHAEFLYKSALEKLNHSEWKR-ISSDEENDENITI 1268


>ref|XP_017971180.1| PREDICTED: separase isoform X1 [Theobroma cacao]
          Length = 2205

 Score =  416 bits (1070), Expect = e-118
 Identities = 278/809 (34%), Positives = 411/809 (50%), Gaps = 122/809 (15%)
 Frame = +2

Query: 1043 CFHCQPLKVKKSMSLQMLIHLKMECXXXXXXXXXXXXXXXXHRARGEINDEQKVLY-SIS 1219
            C+ C P ++ KS  L   I++K E                     G+ ++  KV++ SIS
Sbjct: 1393 CWQCLPTEIMKSGLLNYFINMKWEYAHRKLLVRVLTGIGKCLEYHGQTHELHKVVWQSIS 1452

Query: 1220 VLFSRCLFRESHFPISPEFVTTMIDQNLTGLAFGVEHTSILYKICWHFLKCISNKDTSKK 1399
            VL SR    ++       F+  +I + + G  F VE  +ILY I W  +K I +KDT   
Sbjct: 1453 VLVSRKRITQTCSSAHDTFLLDLIGREILGDVFAVERAAILYSIGWITVKNIHSKDTRAV 1512

Query: 1400 ----SIIATRTIVRGLKIAFIRTRLVPELHQKVARSLAVLY---ASDE--ALSKCDSPCV 1552
                S +     V  LK+AF+  R VP L QKV+R L+ +Y   A+ E  +L  C +   
Sbjct: 1513 CCDLSNVQLSKTVHWLKLAFVLCREVPVLFQKVSRLLSAIYLLSATTELFSLPSCKALSE 1572

Query: 1553 NQWAAYFHQASIGTYLNNHYS-------------DRHQDH------IHQEPVKVEKIE-- 1669
            + WA+YFHQAS+GT+LN  +              D    H      +H E   + ++   
Sbjct: 1573 SHWASYFHQASLGTHLNYQFFPNTCGRPNAQCFVDSRDSHAIGSSCLHTETSTLLRLAPE 1632

Query: 1670 --KDLEASVLEFFSNLPNVAILCISMLEDDSDFLSNLLPHSPTSV--WIVLSRLNSNDVP 1837
              KDLE  V++F++ LP  AI+CIS+L      L   L  +P+ +  W++LSRLNSN+ P
Sbjct: 1633 SVKDLEQFVMDFYACLPCTAIICISLLGHAYTSLLQELLLNPSCIHAWMLLSRLNSNNQP 1692

Query: 1838 IFVMLPVASTFQDSAGFPINKDCSVR------------RPWDCPWVGDTVMDRAAPIFKS 1981
            + ++LP+ S  ++ +      D + R            + W CPW G TV+D  AP FK 
Sbjct: 1693 VVLLLPLDSVLEEVSDDAAPDDDNARACQNLRQHMNSGKKWHCPW-GSTVVDDVAPAFKG 1751

Query: 1982 ILEEFHTTDPNQSV-----------IINKKLDQCLSELLSDIEKKWFGRWKQLLWGEWSD 2128
            ILEE   T  N  +           +I KK+DQ L +LLS++E  W G W+ +L G+  D
Sbjct: 1752 ILEENFITTSNFLIEDTKSTRSLWWMIRKKVDQQLGKLLSNLEDSWLGPWRHVLLGDCLD 1811

Query: 2129 YKNIVC---KLKDHLKSEY-INVDENILKVVLEGAGVNTPKEEFLSELILSNGFYVGTTN 2296
             K++     KL   LKS+  ++++E+ LK+VL GA  +  +E   S   L  G Y+G   
Sbjct: 1812 CKSLNMVHKKLVRDLKSKCKMDINESFLKLVLGGAKYDI-EEACFSWQCLKEGCYIGRLE 1870

Query: 2297 S------HCDGIDKDSFTSISSVVSKTREQMKKKGSTSRLPVILVLDLDIQMLPWENLPI 2458
                     +GIDK S  + S ++ +   ++    + SR P+ILVLD D+QMLPWE++PI
Sbjct: 1871 HPGEEICRSNGIDKVSALA-SQLIHEAVNELHLADTISREPIILVLDYDVQMLPWESIPI 1929

Query: 2459 LRHQDVSRMPSIMSISSTIRKKCSYDDN---DHMVFPTIDPRDAYYVLNPQGDLKQLEHN 2629
            LR Q+V RMPS+ SIS T+ +   Y +    +  VFP IDP DA+Y+LNP GDL   +  
Sbjct: 1930 LRQQEVYRMPSVGSISLTLERSWHYQEQVGRNAAVFPLIDPLDAFYLLNPSGDLSSTQAE 1989

Query: 2630 LAEWFSKQKFEGIVGTSPTQDKLSMILKSRDIFVYIGHGSGTDCISGDIVRNLDRCAAAM 2809
               WF  Q FEG  GT PT ++L+  LKS D+F+Y GHGSG   +S   ++ LD+CAA +
Sbjct: 1990 FENWFRDQNFEGKAGTVPTAEELATALKSHDLFLYFGHGSGEQYLSRKEIQELDKCAATL 2049

Query: 2810 LIGCSSGSLHLNGSYPPLGVVLDYLVAGSPAVVCSLWDITTSHATQITKTLVK------- 2968
            L+GCSSGSL LNG Y P G+ L YL AGSP  + +LW++T     +  K ++        
Sbjct: 2050 LMGCSSGSLVLNGCYMPRGISLSYLRAGSPVTIANLWEVTDKDIDRFGKAVLSAWLSERL 2109

Query: 2969 --------------------------------------------SLMHSKDDKTTVGSSM 3016
                                                        SL ++ D +  +GS +
Sbjct: 2110 EPADCSQCDQLVKEFEAMKIRGRSKGTSRKKVASSNIDETSNGDSLKNTCDHRPKIGSFV 2169

Query: 3017 GSARESCYFPYIQGASLICYGIKTRIKKR 3103
            G ARE+C  P++ GAS +CYG+ T I+++
Sbjct: 2170 GRARETCTLPFLNGASPVCYGVPTGIRRK 2198



 Score =  167 bits (424), Expect = 4e-38
 Identities = 109/336 (32%), Positives = 172/336 (51%), Gaps = 19/336 (5%)
 Frame = +2

Query: 41   ESFDSVEFWKHCMENLEPRI-GFEQKLSILPTISSPDSFQHDHAIH-------INIEEVK 196
            E+  S++FW  C+   +P + GF+Q L     I   ++F H    H       + +  VK
Sbjct: 944  ETSKSIDFWIGCLSGSQPGLLGFQQNL-----ICFFNTFTHRFKTHERDFKSAVPVNNVK 998

Query: 197  QAATNLIDRDPLSIHSWFVAGHLYYDLSETMLEKGSMIXXXXXXXXXXXXRTKLLDKMFI 376
            Q  + LI  DP+  HS F+AG+LYYDL E  +  G +             R++L  + F 
Sbjct: 999  QIVSELISSDPVRSHSLFLAGYLYYDLCERCVSSGQLFEGLSYAKEAFQLRSQLFKRKFA 1058

Query: 377  CHSKKVDKVFDDNGEVIEIKYTY----KTFRMISSVAQTAWSPDKCSSNSKDFIMTPWNV 544
               ++  +  ++ G++ EI        K  ++  +VA   WS D  S +     ++PWNV
Sbjct: 1059 FSIEEQVEKCNETGDIGEIALKVINGPKDLQVQRTVASELWSFDSSSWDLCGCYLSPWNV 1118

Query: 545  LQCFLESILQIG---NLIDDALSAQNILIRGKAISHLQGLDIFSIRFCIALAKLYRKQRK 715
            LQC+LESILQ+G    +  + + A+  L+ GK+IS  Q L +F   F   L KLYRK++ 
Sbjct: 1119 LQCYLESILQVGYINEMTGNGVEAETFLVWGKSISCSQSLPLFEATFSSVLGKLYRKKQL 1178

Query: 716  WIMSEFQLQSALSIWNSSQSHISCVHCKAVLEVSIKQVFADLYIHRFN----NKSKILKE 883
            W  +E +LQSA  I   S S+ SC+ C+ +LE+++ Q   DL+ + F+    N SK  + 
Sbjct: 1179 WHFAEKELQSAKQILVGSSSYYSCIKCRLMLEINLDQQLGDLFCNLFDSTIINNSK--ER 1236

Query: 884  LDDADSFYKDVEKVLNEPIWENDISSPEKSSARKTV 991
            L  A+  YK   + LN   W+  ISS E++    T+
Sbjct: 1237 LSHAEFLYKSALEKLNHSEWKR-ISSDEENDENITI 1271