BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00018743
         (1117 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_023751382.1| pullulanase 1, chloroplastic [Lactuca sativa...   614   0.0  
ref|XP_022006097.1| pullulanase 1, chloroplastic isoform X1 [Hel...   598   0.0  
ref|XP_022006098.1| pullulanase 1, chloroplastic isoform X2 [Hel...   592   0.0  
ref|XP_021690510.1| pullulanase 1, chloroplastic isoform X3 [Hev...   559   0.0  
gb|ESR49767.1| hypothetical protein CICLE_v10031035mg [Citrus cl...   550   0.0  
dbj|GAV67655.1| Alpha-amylase domain-containing protein/CBM_48 d...   559   0.0  
ref|XP_021690509.1| pullulanase 1, chloroplastic isoform X2 [Hev...   559   0.0  
gb|ONI23749.1| hypothetical protein PRUPE_2G205800 [Prunus persica]   551   0.0  
ref|XP_021690508.1| pullulanase 1, chloroplastic isoform X1 [Hev...   559   0.0  
ref|XP_023879675.1| pullulanase 1, chloroplastic isoform X3 [Que...   554   0.0  
gb|OMO65703.1| hypothetical protein CCACVL1_21428, partial [Corc...   549   0.0  
gb|KDP30498.1| hypothetical protein JCGZ_16177 [Jatropha curcas]      551   0.0  
gb|ONI23746.1| hypothetical protein PRUPE_2G205800 [Prunus persica]   551   0.0  
ref|XP_021295296.1| pullulanase 1, chloroplastic isoform X3 [Her...   553   0.0  
ref|XP_021626046.1| pullulanase 1, chloroplastic [Manihot escule...   555   0.0  
ref|XP_015387859.1| PREDICTED: pullulanase 1, chloroplastic isof...   550   0.0  
ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Que...   554   0.0  
ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Que...   554   0.0  
ref|XP_015056540.1| PREDICTED: pullulanase 1, chloroplastic isof...   547   0.0  
ref|XP_021295295.1| pullulanase 1, chloroplastic isoform X2 [Her...   553   0.0  

>ref|XP_023751382.1| pullulanase 1, chloroplastic [Lactuca sativa]
 gb|PLY94815.1| hypothetical protein LSAT_2X103740 [Lactuca sativa]
          Length = 963

 Score =  614 bits (1584), Expect = 0.0
 Identities = 290/326 (88%), Positives = 307/326 (94%)
 Frame = +2

Query: 140  EPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGK 319
            E LEIV L ESNGVWSVNGPRSWEGCYYVYEVSVYHPSTL+IEKCIANDPYARGLSGDGK
Sbjct: 237  ESLEIVNLKESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLKIEKCIANDPYARGLSGDGK 296

Query: 320  RTFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLA 499
            RTFLVNLDSD +KPQKWD+L D+KP+I DF+DISIYELH+RDFSAND TV PEARGGYLA
Sbjct: 297  RTFLVNLDSDALKPQKWDDLADKKPNIVDFSDISIYELHVRDFSANDPTVDPEARGGYLA 356

Query: 500  FTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQ 679
            F+S++SAGVLHLKKLSDAGLTHIHLLPTF FGDV+DEKEKWKSVDMEMLKSLPPDS EQQ
Sbjct: 357  FSSQDSAGVLHLKKLSDAGLTHIHLLPTFHFGDVDDEKEKWKSVDMEMLKSLPPDSPEQQ 416

Query: 680  EYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVV 859
            EYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNG CRI+EFR MVQALN +GLRVVLDVV
Sbjct: 417  EYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGSCRIIEFRKMVQALNFIGLRVVLDVV 476

Query: 860  YNHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINW 1039
            YNHLH +GP  DNSV DKIVPGYYLRRNADGFIENSTC+NNTASEHFMVDRLI+DDL+NW
Sbjct: 477  YNHLHGNGPVGDNSVLDKIVPGYYLRRNADGFIENSTCMNNTASEHFMVDRLIIDDLLNW 536

Query: 1040 VVNYKIDGFRFDLMGHMMKSTMMRAK 1117
             VNYKIDGFRFDLMGHMMKSTM RAK
Sbjct: 537  AVNYKIDGFRFDLMGHMMKSTMTRAK 562



 Score = 68.9 bits (167), Expect = 7e-09
 Identities = 32/40 (80%), Positives = 36/40 (90%)
 Frame = +3

Query: 3   IGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           I VTGLQLPGV+DD+FSY GPLGAIF +E VSL+LWAPTA
Sbjct: 183 ISVTGLQLPGVLDDIFSYLGPLGAIFLNEVVSLHLWAPTA 222


>ref|XP_022006097.1| pullulanase 1, chloroplastic isoform X1 [Helianthus annuus]
 gb|OTF99361.1| putative limit dextrinase [Helianthus annuus]
          Length = 952

 Score =  598 bits (1543), Expect = 0.0
 Identities = 281/322 (87%), Positives = 301/322 (93%)
 Frame = +2

Query: 152  IVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFL 331
            + QL ESNGVWS N PRSWEGCYYVYEVSVYHPSTL+IEKC ANDPYARGLSGDGKRTFL
Sbjct: 232  VAQLKESNGVWSGNVPRSWEGCYYVYEVSVYHPSTLQIEKCFANDPYARGLSGDGKRTFL 291

Query: 332  VNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSE 511
            VNLDSD++KP KWD+L DEKP+IADF+DISIYELH+RDFSAND  V+PE RGGYLAFTS+
Sbjct: 292  VNLDSDDLKPHKWDDLGDEKPNIADFSDISIYELHVRDFSANDPNVHPEIRGGYLAFTSQ 351

Query: 512  ESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYIT 691
            ESAGVLHLKKLSDAGLTHIHLLPTFQF DV+D+KE WK VD EML+SLPPDSAEQQEYIT
Sbjct: 352  ESAGVLHLKKLSDAGLTHIHLLPTFQFDDVDDQKENWKFVDTEMLESLPPDSAEQQEYIT 411

Query: 692  AIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYNHL 871
            +IQNEDGYNWGYNPVLWGVPKGSYATNPNGP RI+EFR MVQALNR+GLRVVLDVVYNHL
Sbjct: 412  SIQNEDGYNWGYNPVLWGVPKGSYATNPNGPSRIIEFRKMVQALNRIGLRVVLDVVYNHL 471

Query: 872  HASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNY 1051
            H SGP  DNSV DKIVPGYYLRRN DGFIENSTCVNNTASEHFMVDRLI+DDL+NWVVNY
Sbjct: 472  HGSGPVGDNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVDRLIIDDLLNWVVNY 531

Query: 1052 KIDGFRFDLMGHMMKSTMMRAK 1117
            KIDGFRFDLMGH+MKSTMM+AK
Sbjct: 532  KIDGFRFDLMGHLMKSTMMKAK 553



 Score = 73.9 bits (180), Expect = 2e-10
 Identities = 33/40 (82%), Positives = 36/40 (90%)
 Frame = +3

Query: 3   IGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           I VTGLQLPGVIDD FSYTGPLGA+FS+E + LYLWAPTA
Sbjct: 174 ISVTGLQLPGVIDDFFSYTGPLGAVFSNEAIDLYLWAPTA 213


>ref|XP_022006098.1| pullulanase 1, chloroplastic isoform X2 [Helianthus annuus]
          Length = 951

 Score =  592 bits (1526), Expect = 0.0
 Identities = 280/322 (86%), Positives = 300/322 (93%)
 Frame = +2

Query: 152  IVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFL 331
            + QL ESNGVWS N PRSWEGCYYVYEVSVYHPSTL+IEKC ANDPYARGLSGDGKRTFL
Sbjct: 232  VAQLKESNGVWSGNVPRSWEGCYYVYEVSVYHPSTLQIEKCFANDPYARGLSGDGKRTFL 291

Query: 332  VNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSE 511
            VNLDSD++KP KWD+L DEKP+IADF+DISIYELH+RDFSAND  V+PE RGGYLAFTS+
Sbjct: 292  VNLDSDDLKPHKWDDLGDEKPNIADFSDISIYELHVRDFSANDPNVHPEIRGGYLAFTSQ 351

Query: 512  ESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYIT 691
             SAGVLHLKKLSDAGLTHIHLLPTFQF DV+D+KE WK VD EML+SLPPDSAEQQEYIT
Sbjct: 352  -SAGVLHLKKLSDAGLTHIHLLPTFQFDDVDDQKENWKFVDTEMLESLPPDSAEQQEYIT 410

Query: 692  AIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYNHL 871
            +IQNEDGYNWGYNPVLWGVPKGSYATNPNGP RI+EFR MVQALNR+GLRVVLDVVYNHL
Sbjct: 411  SIQNEDGYNWGYNPVLWGVPKGSYATNPNGPSRIIEFRKMVQALNRIGLRVVLDVVYNHL 470

Query: 872  HASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNY 1051
            H SGP  DNSV DKIVPGYYLRRN DGFIENSTCVNNTASEHFMVDRLI+DDL+NWVVNY
Sbjct: 471  HGSGPVGDNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVDRLIIDDLLNWVVNY 530

Query: 1052 KIDGFRFDLMGHMMKSTMMRAK 1117
            KIDGFRFDLMGH+MKSTMM+AK
Sbjct: 531  KIDGFRFDLMGHLMKSTMMKAK 552



 Score = 73.9 bits (180), Expect = 2e-10
 Identities = 33/40 (82%), Positives = 36/40 (90%)
 Frame = +3

Query: 3   IGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           I VTGLQLPGVIDD FSYTGPLGA+FS+E + LYLWAPTA
Sbjct: 174 ISVTGLQLPGVIDDFFSYTGPLGAVFSNEAIDLYLWAPTA 213


>ref|XP_021690510.1| pullulanase 1, chloroplastic isoform X3 [Hevea brasiliensis]
          Length = 779

 Score =  559 bits (1441), Expect = 0.0
 Identities = 256/326 (78%), Positives = 289/326 (88%)
 Frame = +2

Query: 140  EPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGK 319
            +P+EIVQL+E NGVWS+ GP  WEGCYYVYEVSVYHP +LRIEKC ANDPYARGLS DGK
Sbjct: 241  DPMEIVQLVEVNGVWSIKGPTDWEGCYYVYEVSVYHPCSLRIEKCFANDPYARGLSSDGK 300

Query: 320  RTFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLA 499
            RT LVNLDSD +KP+ WD L +EKP +  F+DISIYELHIRDFSAND TV P+ RGGYLA
Sbjct: 301  RTLLVNLDSDTLKPEGWDILANEKPTLHSFSDISIYELHIRDFSANDHTVRPDLRGGYLA 360

Query: 500  FTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQ 679
            FT E+SA VLHLKKLS+AG+TH+HLLPTFQF  V+D +E WKSVD  ML+ LPPDS EQQ
Sbjct: 361  FTLEDSASVLHLKKLSNAGITHVHLLPTFQFAGVDDVRENWKSVDNNMLEKLPPDSVEQQ 420

Query: 680  EYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVV 859
             +ITAIQN+DGYNWGYNPVLWGVPKGSYA+NPNGPCR +EFR MVQALN +GLRVVLDVV
Sbjct: 421  AHITAIQNDDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMVQALNHIGLRVVLDVV 480

Query: 860  YNHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINW 1039
            YNHLH SGP D+NSV DK+VPGYYLRRN DGFIENSTCVNNTASEH+MV+RLI+DDL++W
Sbjct: 481  YNHLHGSGPFDENSVLDKVVPGYYLRRNNDGFIENSTCVNNTASEHYMVERLIIDDLLSW 540

Query: 1040 VVNYKIDGFRFDLMGHMMKSTMMRAK 1117
             VNYK+DGFRFDLMGH+MKSTMM+AK
Sbjct: 541  AVNYKVDGFRFDLMGHIMKSTMMKAK 566



 Score = 63.9 bits (154), Expect = 3e-07
 Identities = 28/37 (75%), Positives = 33/37 (89%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++DD+FSY GPLGA FS + VSL+LWAPTA
Sbjct: 190 TGLQLPGILDDLFSYDGPLGADFSTDAVSLHLWAPTA 226


>gb|ESR49767.1| hypothetical protein CICLE_v10031035mg [Citrus clementina]
          Length = 590

 Score =  550 bits (1416), Expect = 0.0
 Identities = 252/325 (77%), Positives = 287/325 (88%)
 Frame = +2

Query: 143  PLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKR 322
            PLE+VQL E++GVWS+ GP+SWEGCYYVYEVSVYHPS L+IEKC ANDPYARGLS DG+R
Sbjct: 246  PLEVVQLKENDGVWSIKGPKSWEGCYYVYEVSVYHPSALQIEKCYANDPYARGLSSDGRR 305

Query: 323  TFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAF 502
            T LVNLDSD +KP+ WD LV EKPDI  F+DISIYELH+RDFS +D TV+P+ RGGYLAF
Sbjct: 306  TLLVNLDSDTLKPEGWDKLVYEKPDILSFSDISIYELHVRDFSVSDHTVHPDFRGGYLAF 365

Query: 503  TSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQE 682
            T + SAGV HLKKLS+AGLTH+HLLPTFQF  V+D KE WKSVD E+L+ LPPDS EQQ 
Sbjct: 366  TLQNSAGVSHLKKLSNAGLTHVHLLPTFQFAGVDDRKENWKSVDAEVLEKLPPDSTEQQA 425

Query: 683  YITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVY 862
             ITAIQN+DGYNWGYNPVLWGVPKGSYA+NPNG CR +EFR MVQALN +GL VVLDVVY
Sbjct: 426  QITAIQNDDGYNWGYNPVLWGVPKGSYASNPNGSCRTIEFRRMVQALNHIGLHVVLDVVY 485

Query: 863  NHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWV 1042
            NHL  SGP DDNSV DK+VPGYYLRRN+DGFIE+STC+NNTASEH+MV+RLI+DDL+ W 
Sbjct: 486  NHLQGSGPFDDNSVLDKVVPGYYLRRNSDGFIEHSTCMNNTASEHYMVERLIIDDLLCWA 545

Query: 1043 VNYKIDGFRFDLMGHMMKSTMMRAK 1117
            VNYK+DGFRFDLMGH+MKSTMM+AK
Sbjct: 546  VNYKVDGFRFDLMGHIMKSTMMKAK 570



 Score = 67.0 bits (162), Expect = 2e-08
 Identities = 27/37 (72%), Positives = 35/37 (94%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++D++FSY GPLGA++++E VSLYLWAPTA
Sbjct: 194 TGLQLPGILDELFSYDGPLGALYAEETVSLYLWAPTA 230


>dbj|GAV67655.1| Alpha-amylase domain-containing protein/CBM_48 domain-containing
            protein/DUF3372 domain-containing protein, partial
            [Cephalotus follicularis]
          Length = 857

 Score =  559 bits (1440), Expect = 0.0
 Identities = 256/326 (78%), Positives = 291/326 (89%)
 Frame = +2

Query: 140  EPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGK 319
            +P EIVQL E NGVWS  GP+SWEGCYYVYEVSVYHPST++IEKC ANDPYARGLS DG+
Sbjct: 238  DPQEIVQLQEVNGVWSTKGPKSWEGCYYVYEVSVYHPSTMQIEKCYANDPYARGLSSDGR 297

Query: 320  RTFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLA 499
            RT LVNLDSD +KP+ WD + DEKPDI  F+DISIYELHIRDFSAND TV+P+ RG Y+A
Sbjct: 298  RTLLVNLDSDTLKPEGWDKVADEKPDIISFSDISIYELHIRDFSANDHTVHPDFRGCYMA 357

Query: 500  FTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQ 679
            FT ++SAG+LHLKKLS+AG+TH+HLLPTF F  V DEKEKWK+VD E+L+ LPPDS EQQ
Sbjct: 358  FTLQDSAGMLHLKKLSNAGITHVHLLPTFHFAGVVDEKEKWKNVDAEILEKLPPDSTEQQ 417

Query: 680  EYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVV 859
              ITAIQN+DGYNWGYNPVLWGVPKGSYATNPNGPCR +EFR MVQALNR+GLRVVLDVV
Sbjct: 418  AQITAIQNDDGYNWGYNPVLWGVPKGSYATNPNGPCRTIEFRKMVQALNRIGLRVVLDVV 477

Query: 860  YNHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINW 1039
            YNHLH +GP D+NSV DKIVPGYYLRRN DGFIE+STCVNNTASEH+MV+RLI+DDL+ W
Sbjct: 478  YNHLHGNGPFDENSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASEHYMVERLILDDLLCW 537

Query: 1040 VVNYKIDGFRFDLMGHMMKSTMMRAK 1117
             +NYK+DGFRFDLMGH+MKSTMM+AK
Sbjct: 538  AINYKVDGFRFDLMGHIMKSTMMKAK 563



 Score = 64.7 bits (156), Expect = 2e-07
 Identities = 26/37 (70%), Positives = 35/37 (94%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++D++FSY GPLGA+F +E+V+L+LWAPTA
Sbjct: 187 TGLQLPGILDELFSYDGPLGALFLEESVTLFLWAPTA 223


>ref|XP_021690509.1| pullulanase 1, chloroplastic isoform X2 [Hevea brasiliensis]
          Length = 946

 Score =  559 bits (1441), Expect = 0.0
 Identities = 256/326 (78%), Positives = 289/326 (88%)
 Frame = +2

Query: 140  EPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGK 319
            +P+EIVQL+E NGVWS+ GP  WEGCYYVYEVSVYHP +LRIEKC ANDPYARGLS DGK
Sbjct: 241  DPMEIVQLVEVNGVWSIKGPTDWEGCYYVYEVSVYHPCSLRIEKCFANDPYARGLSSDGK 300

Query: 320  RTFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLA 499
            RT LVNLDSD +KP+ WD L +EKP +  F+DISIYELHIRDFSAND TV P+ RGGYLA
Sbjct: 301  RTLLVNLDSDTLKPEGWDILANEKPTLHSFSDISIYELHIRDFSANDHTVRPDLRGGYLA 360

Query: 500  FTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQ 679
            FT E+SA VLHLKKLS+AG+TH+HLLPTFQF  V+D +E WKSVD  ML+ LPPDS EQQ
Sbjct: 361  FTLEDSASVLHLKKLSNAGITHVHLLPTFQFAGVDDVRENWKSVDNNMLEKLPPDSVEQQ 420

Query: 680  EYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVV 859
             +ITAIQN+DGYNWGYNPVLWGVPKGSYA+NPNGPCR +EFR MVQALN +GLRVVLDVV
Sbjct: 421  AHITAIQNDDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMVQALNHIGLRVVLDVV 480

Query: 860  YNHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINW 1039
            YNHLH SGP D+NSV DK+VPGYYLRRN DGFIENSTCVNNTASEH+MV+RLI+DDL++W
Sbjct: 481  YNHLHGSGPFDENSVLDKVVPGYYLRRNNDGFIENSTCVNNTASEHYMVERLIIDDLLSW 540

Query: 1040 VVNYKIDGFRFDLMGHMMKSTMMRAK 1117
             VNYK+DGFRFDLMGH+MKSTMM+AK
Sbjct: 541  AVNYKVDGFRFDLMGHIMKSTMMKAK 566



 Score = 63.9 bits (154), Expect = 3e-07
 Identities = 28/37 (75%), Positives = 33/37 (89%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++DD+FSY GPLGA FS + VSL+LWAPTA
Sbjct: 190 TGLQLPGILDDLFSYDGPLGADFSTDAVSLHLWAPTA 226


>gb|ONI23749.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 697

 Score =  551 bits (1419), Expect = 0.0
 Identities = 255/325 (78%), Positives = 286/325 (88%)
 Frame = +2

Query: 143  PLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKR 322
            PLE+VQL E NGVWS  GP+SWEGCYYVYEVSVYHPSTLRIEKC ANDPYARGLS DG+R
Sbjct: 242  PLEVVQLEEFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGRR 301

Query: 323  TFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAF 502
            T LVNLDSDN+KP+ WD LVDEKPDI  F+DISIYELHIRDFSA+D  V+PE RGGYLAF
Sbjct: 302  TLLVNLDSDNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHPEFRGGYLAF 361

Query: 503  TSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQE 682
            T ++SAG +HLKKLS+AG+TH+HLLP FQF  V+DEKE WKSVD E+L+  P DS +QQ 
Sbjct: 362  TLQDSAGAIHLKKLSNAGITHVHLLPAFQFAGVDDEKENWKSVDPEVLEKFPSDSDKQQA 421

Query: 683  YITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVY 862
             ITAIQN+DGYNWGYNPVLWGVPKGSYA+N NG  R +EFR MVQALNR GLRVVLDVVY
Sbjct: 422  LITAIQNDDGYNWGYNPVLWGVPKGSYASNANGTYRAIEFRKMVQALNRFGLRVVLDVVY 481

Query: 863  NHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWV 1042
            NHLH SGP DDNSV DKIVPGYYLRRN DGFIE+STCVNNTASEHFMV+RLIVDDL++W 
Sbjct: 482  NHLHGSGPVDDNSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASEHFMVERLIVDDLLHWA 541

Query: 1043 VNYKIDGFRFDLMGHMMKSTMMRAK 1117
            V+YK+DGFRFDLMGH+M+ TM++AK
Sbjct: 542  VDYKVDGFRFDLMGHIMRRTMVKAK 566



 Score = 68.2 bits (165), Expect = 1e-08
 Identities = 28/37 (75%), Positives = 35/37 (94%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++DD+FSY GPLGA++S ++VSLYLWAPTA
Sbjct: 190 TGLQLPGILDDLFSYNGPLGALYSKQSVSLYLWAPTA 226


>ref|XP_021690508.1| pullulanase 1, chloroplastic isoform X1 [Hevea brasiliensis]
          Length = 965

 Score =  559 bits (1441), Expect = 0.0
 Identities = 256/326 (78%), Positives = 289/326 (88%)
 Frame = +2

Query: 140  EPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGK 319
            +P+EIVQL+E NGVWS+ GP  WEGCYYVYEVSVYHP +LRIEKC ANDPYARGLS DGK
Sbjct: 241  DPMEIVQLVEVNGVWSIKGPTDWEGCYYVYEVSVYHPCSLRIEKCFANDPYARGLSSDGK 300

Query: 320  RTFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLA 499
            RT LVNLDSD +KP+ WD L +EKP +  F+DISIYELHIRDFSAND TV P+ RGGYLA
Sbjct: 301  RTLLVNLDSDTLKPEGWDILANEKPTLHSFSDISIYELHIRDFSANDHTVRPDLRGGYLA 360

Query: 500  FTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQ 679
            FT E+SA VLHLKKLS+AG+TH+HLLPTFQF  V+D +E WKSVD  ML+ LPPDS EQQ
Sbjct: 361  FTLEDSASVLHLKKLSNAGITHVHLLPTFQFAGVDDVRENWKSVDNNMLEKLPPDSVEQQ 420

Query: 680  EYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVV 859
             +ITAIQN+DGYNWGYNPVLWGVPKGSYA+NPNGPCR +EFR MVQALN +GLRVVLDVV
Sbjct: 421  AHITAIQNDDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMVQALNHIGLRVVLDVV 480

Query: 860  YNHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINW 1039
            YNHLH SGP D+NSV DK+VPGYYLRRN DGFIENSTCVNNTASEH+MV+RLI+DDL++W
Sbjct: 481  YNHLHGSGPFDENSVLDKVVPGYYLRRNNDGFIENSTCVNNTASEHYMVERLIIDDLLSW 540

Query: 1040 VVNYKIDGFRFDLMGHMMKSTMMRAK 1117
             VNYK+DGFRFDLMGH+MKSTMM+AK
Sbjct: 541  AVNYKVDGFRFDLMGHIMKSTMMKAK 566



 Score = 63.9 bits (154), Expect = 3e-07
 Identities = 28/37 (75%), Positives = 33/37 (89%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++DD+FSY GPLGA FS + VSL+LWAPTA
Sbjct: 190 TGLQLPGILDDLFSYDGPLGADFSTDAVSLHLWAPTA 226


>ref|XP_023879675.1| pullulanase 1, chloroplastic isoform X3 [Quercus suber]
          Length = 825

 Score =  554 bits (1427), Expect = 0.0
 Identities = 254/324 (78%), Positives = 290/324 (89%)
 Frame = +2

Query: 146  LEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRT 325
            LEIVQL E NGVWS  GP+ WEGCYYVYEVSVYHPSTLRI+KC  NDPYARGLS DG+RT
Sbjct: 243  LEIVQLEEVNGVWSTKGPKRWEGCYYVYEVSVYHPSTLRIDKCHVNDPYARGLSSDGRRT 302

Query: 326  FLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFT 505
            FLVNLDSD +KP++WDNL +EKPDI  F+DISIYELHIRDFSAND TV+PE RGGYLAFT
Sbjct: 303  FLVNLDSDTLKPEEWDNLANEKPDILSFSDISIYELHIRDFSANDYTVHPEFRGGYLAFT 362

Query: 506  SEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEY 685
             ++S+GVLHLKKLS+AGLTH+HLLPTFQF  V+DEKE WK VD  ML+ LPPDS +QQ+ 
Sbjct: 363  LQDSSGVLHLKKLSNAGLTHVHLLPTFQFAGVDDEKENWKCVDTNMLEKLPPDSVQQQDL 422

Query: 686  ITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYN 865
            ITAIQ+ DGYNWGYNPVLWGVPKGSYA+NPNGP RI+EFR M+QALNR+GLR+VLDVVYN
Sbjct: 423  ITAIQDNDGYNWGYNPVLWGVPKGSYASNPNGPYRIIEFRKMIQALNRIGLRIVLDVVYN 482

Query: 866  HLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVV 1045
            H+H SGP D+NSV DKIVPGYYLR+N DG IENSTCVNNTASEH MV+RLI+DDL++W V
Sbjct: 483  HVHGSGPFDENSVLDKIVPGYYLRKNTDGLIENSTCVNNTASEHVMVERLILDDLLHWAV 542

Query: 1046 NYKIDGFRFDLMGHMMKSTMMRAK 1117
            +YK+DGFRFDLMGH+MKSTM++AK
Sbjct: 543  HYKVDGFRFDLMGHIMKSTMVKAK 566



 Score = 73.9 bits (180), Expect = 2e-10
 Identities = 32/40 (80%), Positives = 38/40 (95%)
 Frame = +3

Query: 3   IGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           I VTGLQLPGV+DD+FSY GPLGA++S+E+VSLYLWAPTA
Sbjct: 187 INVTGLQLPGVLDDLFSYNGPLGALYSEESVSLYLWAPTA 226


>gb|OMO65703.1| hypothetical protein CCACVL1_21428, partial [Corchorus capsularis]
          Length = 702

 Score =  549 bits (1415), Expect = 0.0
 Identities = 252/325 (77%), Positives = 288/325 (88%)
 Frame = +2

Query: 143  PLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKR 322
            PLEI+ L E+NGVWS  GP+SWEGCYYVYEVSVYHPSTL+IEKC ANDPYARGLS DG+R
Sbjct: 271  PLEIIPLEETNGVWSTKGPKSWEGCYYVYEVSVYHPSTLQIEKCYANDPYARGLSSDGRR 330

Query: 323  TFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAF 502
            T  VNLD++ +KP+ WD L D+KPDI  F+DISIYELHIRDFSAND+TVYPE RGGYLAF
Sbjct: 331  TLFVNLDTNGLKPEGWDELADKKPDIDSFSDISIYELHIRDFSANDNTVYPEFRGGYLAF 390

Query: 503  TSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQE 682
            T E+SAGVLHLKKLS+AG+TH+HLLPTFQF  V+DEKE WK VD ++++ L PDS EQQ 
Sbjct: 391  TLEDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDEKENWKHVDSKIMEKLSPDSDEQQM 450

Query: 683  YITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVY 862
             ITAIQN+D YNWGYNPVLWGVPKGSYA++PNGPCRI+EFR M+QALN +GL VVLDVVY
Sbjct: 451  QITAIQNDDAYNWGYNPVLWGVPKGSYASDPNGPCRIIEFRKMIQALNHIGLCVVLDVVY 510

Query: 863  NHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWV 1042
            NHLHASGP D +SV DKIVPGYYLRRN DGFIENSTCVNNTASEH+MV RLIVDDL++W 
Sbjct: 511  NHLHASGPVDKDSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHYMVGRLIVDDLLSWA 570

Query: 1043 VNYKIDGFRFDLMGHMMKSTMMRAK 1117
            +NYK+DGFRFDLMGH+MK TM++AK
Sbjct: 571  MNYKVDGFRFDLMGHIMKRTMVKAK 595



 Score = 67.4 bits (163), Expect = 2e-08
 Identities = 28/37 (75%), Positives = 35/37 (94%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++D++FSY GPLGA++S E+VSLYLWAPTA
Sbjct: 219 TGLQLPGILDELFSYDGPLGALYSGESVSLYLWAPTA 255


>gb|KDP30498.1| hypothetical protein JCGZ_16177 [Jatropha curcas]
          Length = 774

 Score =  551 bits (1420), Expect = 0.0
 Identities = 254/326 (77%), Positives = 290/326 (88%)
 Frame = +2

Query: 140  EPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGK 319
            EPLEI QL+E +GVWS+ GP+ WEGCYYVYEVSVYHPSTLRIEKC ANDPYARGLS DGK
Sbjct: 50   EPLEIGQLMEVDGVWSITGPKDWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGK 109

Query: 320  RTFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLA 499
            RT LVNLDS+  KP+ WD L DEKP +  F++ISIYELHIRDFSAND TV+P++RGGYLA
Sbjct: 110  RTLLVNLDSNATKPEGWDELSDEKPTLLSFSNISIYELHIRDFSANDETVHPDSRGGYLA 169

Query: 500  FTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQ 679
            FT E+SAGVLHLKKLS+AG+TH+HLLP FQF +V+D KE WK VD +ML+ L PDS+EQQ
Sbjct: 170  FTLEDSAGVLHLKKLSNAGITHVHLLPAFQFAEVDDVKENWKRVDNKMLEHLSPDSSEQQ 229

Query: 680  EYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVV 859
              ITAIQ+ DGYNWGYNPVLWGVPKGSYA+NP+G CR +EFR MVQALNR+GLRVVLDVV
Sbjct: 230  ALITAIQDNDGYNWGYNPVLWGVPKGSYASNPDGACRTIEFRKMVQALNRIGLRVVLDVV 289

Query: 860  YNHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINW 1039
            YNHLH +GP D NSV DK+VPGYYLRRN DGFIENSTCVNNTASEH+MV+RLI+DDL+NW
Sbjct: 290  YNHLHGNGPFDGNSVLDKVVPGYYLRRNNDGFIENSTCVNNTASEHYMVERLIIDDLLNW 349

Query: 1040 VVNYKIDGFRFDLMGHMMKSTMMRAK 1117
            VVNYK+DGFRFDLMGH+MKSTM++AK
Sbjct: 350  VVNYKVDGFRFDLMGHIMKSTMVKAK 375


>gb|ONI23746.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 777

 Score =  551 bits (1419), Expect = 0.0
 Identities = 255/325 (78%), Positives = 286/325 (88%)
 Frame = +2

Query: 143  PLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKR 322
            PLE+VQL E NGVWS  GP+SWEGCYYVYEVSVYHPSTLRIEKC ANDPYARGLS DG+R
Sbjct: 242  PLEVVQLEEFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGRR 301

Query: 323  TFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAF 502
            T LVNLDSDN+KP+ WD LVDEKPDI  F+DISIYELHIRDFSA+D  V+PE RGGYLAF
Sbjct: 302  TLLVNLDSDNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHPEFRGGYLAF 361

Query: 503  TSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQE 682
            T ++SAG +HLKKLS+AG+TH+HLLP FQF  V+DEKE WKSVD E+L+  P DS +QQ 
Sbjct: 362  TLQDSAGAIHLKKLSNAGITHVHLLPAFQFAGVDDEKENWKSVDPEVLEKFPSDSDKQQA 421

Query: 683  YITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVY 862
             ITAIQN+DGYNWGYNPVLWGVPKGSYA+N NG  R +EFR MVQALNR GLRVVLDVVY
Sbjct: 422  LITAIQNDDGYNWGYNPVLWGVPKGSYASNANGTYRAIEFRKMVQALNRFGLRVVLDVVY 481

Query: 863  NHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWV 1042
            NHLH SGP DDNSV DKIVPGYYLRRN DGFIE+STCVNNTASEHFMV+RLIVDDL++W 
Sbjct: 482  NHLHGSGPVDDNSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASEHFMVERLIVDDLLHWA 541

Query: 1043 VNYKIDGFRFDLMGHMMKSTMMRAK 1117
            V+YK+DGFRFDLMGH+M+ TM++AK
Sbjct: 542  VDYKVDGFRFDLMGHIMRRTMVKAK 566



 Score = 68.2 bits (165), Expect = 1e-08
 Identities = 28/37 (75%), Positives = 35/37 (94%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++DD+FSY GPLGA++S ++VSLYLWAPTA
Sbjct: 190 TGLQLPGILDDLFSYNGPLGALYSKQSVSLYLWAPTA 226


>ref|XP_021295296.1| pullulanase 1, chloroplastic isoform X3 [Herrania umbratica]
          Length = 877

 Score =  553 bits (1425), Expect = 0.0
 Identities = 251/325 (77%), Positives = 291/325 (89%)
 Frame = +2

Query: 143  PLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKR 322
            PLEI+QL+E+NGVWS  GP+ WEGCYYVYEVSVYHPSTL+IEKC ANDPYARGLS DG+R
Sbjct: 236  PLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYANDPYARGLSSDGRR 295

Query: 323  TFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAF 502
            T  VNLDS+++KP+ WD L D+KPDI  F+DISIYELHIRDFSAND TV P+  GGY+AF
Sbjct: 296  TLFVNLDSNSLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDKTVNPDFCGGYMAF 355

Query: 503  TSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQE 682
            T ++SAGVLHLKKLS+AG+TH+HLLPTFQF  V+DE E WK VD ++LK LPPDSAEQQ 
Sbjct: 356  TLQDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDESENWKYVDYKILKKLPPDSAEQQV 415

Query: 683  YITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVY 862
             ITAIQN+D YNWGYNPVLWGVPKGSYA++PNGPCRI+EFR M+QALN +GLR+VLDVVY
Sbjct: 416  QITAIQNDDAYNWGYNPVLWGVPKGSYASDPNGPCRIIEFRKMIQALNHIGLRIVLDVVY 475

Query: 863  NHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWV 1042
            NHLHASGP D +SV DKIVPGYYLRRN DGFIENSTCVNNTASEH+MV+RLI+DDL++W 
Sbjct: 476  NHLHASGPFDKDSVLDKIVPGYYLRRNNDGFIENSTCVNNTASEHYMVERLIIDDLLSWS 535

Query: 1043 VNYKIDGFRFDLMGHMMKSTMMRAK 1117
            +NYK+DGFRFDLMGH+MKSTM++AK
Sbjct: 536  INYKVDGFRFDLMGHIMKSTMVKAK 560



 Score = 64.3 bits (155), Expect = 2e-07
 Identities = 27/37 (72%), Positives = 33/37 (89%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++D++FSY GPLGA + +E VSLYLWAPTA
Sbjct: 184 TGLQLPGILDELFSYDGPLGAFYLEEAVSLYLWAPTA 220


>ref|XP_021626046.1| pullulanase 1, chloroplastic [Manihot esculenta]
 gb|OAY38904.1| hypothetical protein MANES_10G051700 [Manihot esculenta]
          Length = 966

 Score =  555 bits (1431), Expect = 0.0
 Identities = 257/325 (79%), Positives = 286/325 (88%)
 Frame = +2

Query: 143  PLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKR 322
            P E VQL+E NGVWS  GP+ WEGCYYVYEVSVYHPSTL IEK  ANDPYARGLS DGKR
Sbjct: 243  PTETVQLMEVNGVWSTKGPKDWEGCYYVYEVSVYHPSTLHIEKYFANDPYARGLSSDGKR 302

Query: 323  TFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAF 502
            T LVNLDSD +KP++WD L DEKP +  F+DISIYELHIRDFSAND TV+ + RGGYLAF
Sbjct: 303  TLLVNLDSDTLKPERWDILADEKPTLLSFSDISIYELHIRDFSANDDTVHSDLRGGYLAF 362

Query: 503  TSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQE 682
            T E+SAGVLHLKKLS AGLTH+HLLP FQF  V+D +E WKSVD  ML+ LPPDSAEQQ 
Sbjct: 363  TVEDSAGVLHLKKLSSAGLTHVHLLPAFQFAGVDDVRENWKSVDNSMLEKLPPDSAEQQA 422

Query: 683  YITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVY 862
            +ITA QN+DGYNWGYNPVLWGVPKGSYA+NPNG CR +EFR MVQA+NR+GLRVVLDVVY
Sbjct: 423  HITAFQNDDGYNWGYNPVLWGVPKGSYASNPNGSCRTIEFRKMVQAINRIGLRVVLDVVY 482

Query: 863  NHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWV 1042
            NHLH SGP D+NSV DK+VPGYYLRRN+DGFIENSTCVNNTASEH+MV+RLIVDDL++W 
Sbjct: 483  NHLHGSGPFDENSVLDKVVPGYYLRRNSDGFIENSTCVNNTASEHYMVERLIVDDLLSWA 542

Query: 1043 VNYKIDGFRFDLMGHMMKSTMMRAK 1117
            VNYKIDGFRFDLMGHMMKSTM++AK
Sbjct: 543  VNYKIDGFRFDLMGHMMKSTMVKAK 567



 Score = 63.2 bits (152), Expect = 5e-07
 Identities = 27/37 (72%), Positives = 33/37 (89%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++D++FSY GPLGA F+ + VSLYLWAPTA
Sbjct: 191 TGLQLPGILDELFSYDGPLGAHFAKDAVSLYLWAPTA 227


>ref|XP_015387859.1| PREDICTED: pullulanase 1, chloroplastic isoform X4 [Citrus sinensis]
 ref|XP_024042245.1| pullulanase 1, chloroplastic isoform X4 [Citrus clementina]
          Length = 817

 Score =  550 bits (1416), Expect = 0.0
 Identities = 252/325 (77%), Positives = 287/325 (88%)
 Frame = +2

Query: 143  PLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKR 322
            PLE+VQL E++GVWS+ GP+SWEGCYYVYEVSVYHPS L+IEKC ANDPYARGLS DG+R
Sbjct: 246  PLEVVQLKENDGVWSIKGPKSWEGCYYVYEVSVYHPSALQIEKCYANDPYARGLSSDGRR 305

Query: 323  TFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAF 502
            T LVNLDSD +KP+ WD LV EKPDI  F+DISIYELH+RDFS +D TV+P+ RGGYLAF
Sbjct: 306  TLLVNLDSDTLKPEGWDKLVYEKPDILSFSDISIYELHVRDFSVSDHTVHPDFRGGYLAF 365

Query: 503  TSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQE 682
            T + SAGV HLKKLS+AGLTH+HLLPTFQF  V+D KE WKSVD E+L+ LPPDS EQQ 
Sbjct: 366  TLQNSAGVSHLKKLSNAGLTHVHLLPTFQFAGVDDRKENWKSVDAEVLEKLPPDSTEQQA 425

Query: 683  YITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVY 862
             ITAIQN+DGYNWGYNPVLWGVPKGSYA+NPNG CR +EFR MVQALN +GL VVLDVVY
Sbjct: 426  QITAIQNDDGYNWGYNPVLWGVPKGSYASNPNGSCRTIEFRRMVQALNHIGLHVVLDVVY 485

Query: 863  NHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWV 1042
            NHL  SGP DDNSV DK+VPGYYLRRN+DGFIE+STC+NNTASEH+MV+RLI+DDL+ W 
Sbjct: 486  NHLQGSGPFDDNSVLDKVVPGYYLRRNSDGFIEHSTCMNNTASEHYMVERLIIDDLLCWA 545

Query: 1043 VNYKIDGFRFDLMGHMMKSTMMRAK 1117
            VNYK+DGFRFDLMGH+MKSTMM+AK
Sbjct: 546  VNYKVDGFRFDLMGHIMKSTMMKAK 570



 Score = 67.0 bits (162), Expect = 3e-08
 Identities = 27/37 (72%), Positives = 35/37 (94%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++D++FSY GPLGA++++E VSLYLWAPTA
Sbjct: 194 TGLQLPGILDELFSYDGPLGALYAEETVSLYLWAPTA 230


>ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Quercus suber]
 gb|POE76574.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 961

 Score =  554 bits (1427), Expect = 0.0
 Identities = 254/324 (78%), Positives = 290/324 (89%)
 Frame = +2

Query: 146  LEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRT 325
            LEIVQL E NGVWS  GP+ WEGCYYVYEVSVYHPSTLRI+KC  NDPYARGLS DG+RT
Sbjct: 241  LEIVQLEEVNGVWSTKGPKRWEGCYYVYEVSVYHPSTLRIDKCHVNDPYARGLSSDGRRT 300

Query: 326  FLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFT 505
            FLVNLDSD +KP++WDNL +EKPDI  F+DISIYELHIRDFSAND TV+PE RGGYLAFT
Sbjct: 301  FLVNLDSDTLKPEEWDNLANEKPDILSFSDISIYELHIRDFSANDYTVHPEFRGGYLAFT 360

Query: 506  SEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEY 685
             ++S+GVLHLKKLS+AGLTH+HLLPTFQF  V+DEKE WK VD  ML+ LPPDS +QQ+ 
Sbjct: 361  LQDSSGVLHLKKLSNAGLTHVHLLPTFQFAGVDDEKENWKCVDTNMLEKLPPDSVQQQDL 420

Query: 686  ITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYN 865
            ITAIQ+ DGYNWGYNPVLWGVPKGSYA+NPNGP RI+EFR M+QALNR+GLR+VLDVVYN
Sbjct: 421  ITAIQDNDGYNWGYNPVLWGVPKGSYASNPNGPYRIIEFRKMIQALNRIGLRIVLDVVYN 480

Query: 866  HLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVV 1045
            H+H SGP D+NSV DKIVPGYYLR+N DG IENSTCVNNTASEH MV+RLI+DDL++W V
Sbjct: 481  HVHGSGPFDENSVLDKIVPGYYLRKNTDGLIENSTCVNNTASEHVMVERLILDDLLHWAV 540

Query: 1046 NYKIDGFRFDLMGHMMKSTMMRAK 1117
            +YK+DGFRFDLMGH+MKSTM++AK
Sbjct: 541  HYKVDGFRFDLMGHIMKSTMVKAK 564



 Score = 73.9 bits (180), Expect = 2e-10
 Identities = 32/40 (80%), Positives = 38/40 (95%)
 Frame = +3

Query: 3   IGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           I VTGLQLPGV+DD+FSY GPLGA++S+E+VSLYLWAPTA
Sbjct: 185 INVTGLQLPGVLDDLFSYNGPLGALYSEESVSLYLWAPTA 224


>ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Quercus suber]
 gb|POE76573.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 963

 Score =  554 bits (1427), Expect = 0.0
 Identities = 254/324 (78%), Positives = 290/324 (89%)
 Frame = +2

Query: 146  LEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRT 325
            LEIVQL E NGVWS  GP+ WEGCYYVYEVSVYHPSTLRI+KC  NDPYARGLS DG+RT
Sbjct: 243  LEIVQLEEVNGVWSTKGPKRWEGCYYVYEVSVYHPSTLRIDKCHVNDPYARGLSSDGRRT 302

Query: 326  FLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFT 505
            FLVNLDSD +KP++WDNL +EKPDI  F+DISIYELHIRDFSAND TV+PE RGGYLAFT
Sbjct: 303  FLVNLDSDTLKPEEWDNLANEKPDILSFSDISIYELHIRDFSANDYTVHPEFRGGYLAFT 362

Query: 506  SEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEY 685
             ++S+GVLHLKKLS+AGLTH+HLLPTFQF  V+DEKE WK VD  ML+ LPPDS +QQ+ 
Sbjct: 363  LQDSSGVLHLKKLSNAGLTHVHLLPTFQFAGVDDEKENWKCVDTNMLEKLPPDSVQQQDL 422

Query: 686  ITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYN 865
            ITAIQ+ DGYNWGYNPVLWGVPKGSYA+NPNGP RI+EFR M+QALNR+GLR+VLDVVYN
Sbjct: 423  ITAIQDNDGYNWGYNPVLWGVPKGSYASNPNGPYRIIEFRKMIQALNRIGLRIVLDVVYN 482

Query: 866  HLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVV 1045
            H+H SGP D+NSV DKIVPGYYLR+N DG IENSTCVNNTASEH MV+RLI+DDL++W V
Sbjct: 483  HVHGSGPFDENSVLDKIVPGYYLRKNTDGLIENSTCVNNTASEHVMVERLILDDLLHWAV 542

Query: 1046 NYKIDGFRFDLMGHMMKSTMMRAK 1117
            +YK+DGFRFDLMGH+MKSTM++AK
Sbjct: 543  HYKVDGFRFDLMGHIMKSTMVKAK 566



 Score = 73.9 bits (180), Expect = 2e-10
 Identities = 32/40 (80%), Positives = 38/40 (95%)
 Frame = +3

Query: 3   IGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           I VTGLQLPGV+DD+FSY GPLGA++S+E+VSLYLWAPTA
Sbjct: 187 INVTGLQLPGVLDDLFSYNGPLGALYSEESVSLYLWAPTA 226


>ref|XP_015056540.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Solanum
            pennellii]
          Length = 778

 Score =  547 bits (1410), Expect = 0.0
 Identities = 256/326 (78%), Positives = 288/326 (88%)
 Frame = +2

Query: 140  EPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGK 319
            +P+EIVQL E +GVWS  GPR WEGCYYVYEVSVYHPSTLRIEKC++NDPYARGLS DGK
Sbjct: 230  DPVEIVQLKELDGVWSAKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGK 289

Query: 320  RTFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLA 499
            RT LVNLDSD+VKP+ WDNL DEKP++  F+D+SIYELH+RDFSAND TV  E +GGYLA
Sbjct: 290  RTLLVNLDSDDVKPEGWDNLQDEKPNLLSFSDVSIYELHVRDFSANDPTVPHEFQGGYLA 349

Query: 500  FTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQ 679
            FTS++SAGV HLK+LS+AG+TH+HLLPT+QF  VEDEK KWK  D+E L S PPDS EQQ
Sbjct: 350  FTSQDSAGVQHLKRLSNAGITHVHLLPTYQFAGVEDEKHKWKYTDIEKLNSFPPDSEEQQ 409

Query: 680  EYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVV 859
              ITAIQ+EDGYNWGYNPVLWGVPKGSYA+N NGPCRI+EFR MVQALNR+GLRVVLDVV
Sbjct: 410  ALITAIQDEDGYNWGYNPVLWGVPKGSYASNANGPCRIVEFRKMVQALNRIGLRVVLDVV 469

Query: 860  YNHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINW 1039
            YNHLHASGP  D SV DKIVPGY+LRRNADG IENSTCVNNTASEHFMV+RLI+DDL  W
Sbjct: 470  YNHLHASGPFSDFSVLDKIVPGYFLRRNADGAIENSTCVNNTASEHFMVERLILDDLKCW 529

Query: 1040 VVNYKIDGFRFDLMGHMMKSTMMRAK 1117
             V+YKIDGFRFDLMGH+MK TM++AK
Sbjct: 530  AVHYKIDGFRFDLMGHIMKRTMLKAK 555



 Score = 67.8 bits (164), Expect = 2e-08
 Identities = 29/37 (78%), Positives = 34/37 (91%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++D++FSYTGPLG IF+ E VSLYLWAPTA
Sbjct: 179 TGLQLPGILDELFSYTGPLGGIFATEAVSLYLWAPTA 215


>ref|XP_021295295.1| pullulanase 1, chloroplastic isoform X2 [Herrania umbratica]
          Length = 963

 Score =  553 bits (1425), Expect = 0.0
 Identities = 251/325 (77%), Positives = 291/325 (89%)
 Frame = +2

Query: 143  PLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKR 322
            PLEI+QL+E+NGVWS  GP+ WEGCYYVYEVSVYHPSTL+IEKC ANDPYARGLS DG+R
Sbjct: 234  PLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYANDPYARGLSSDGRR 293

Query: 323  TFLVNLDSDNVKPQKWDNLVDEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAF 502
            T  VNLDS+++KP+ WD L D+KPDI  F+DISIYELHIRDFSAND TV P+  GGY+AF
Sbjct: 294  TLFVNLDSNSLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDKTVNPDFCGGYMAF 353

Query: 503  TSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQE 682
            T ++SAGVLHLKKLS+AG+TH+HLLPTFQF  V+DE E WK VD ++LK LPPDSAEQQ 
Sbjct: 354  TLQDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDESENWKYVDYKILKKLPPDSAEQQV 413

Query: 683  YITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVY 862
             ITAIQN+D YNWGYNPVLWGVPKGSYA++PNGPCRI+EFR M+QALN +GLR+VLDVVY
Sbjct: 414  QITAIQNDDAYNWGYNPVLWGVPKGSYASDPNGPCRIIEFRKMIQALNHIGLRIVLDVVY 473

Query: 863  NHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWV 1042
            NHLHASGP D +SV DKIVPGYYLRRN DGFIENSTCVNNTASEH+MV+RLI+DDL++W 
Sbjct: 474  NHLHASGPFDKDSVLDKIVPGYYLRRNNDGFIENSTCVNNTASEHYMVERLIIDDLLSWS 533

Query: 1043 VNYKIDGFRFDLMGHMMKSTMMRAK 1117
            +NYK+DGFRFDLMGH+MKSTM++AK
Sbjct: 534  INYKVDGFRFDLMGHIMKSTMVKAK 558



 Score = 64.3 bits (155), Expect = 2e-07
 Identities = 27/37 (72%), Positives = 33/37 (89%)
 Frame = +3

Query: 12  TGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTA 122
           TGLQLPG++D++FSY GPLGA + +E VSLYLWAPTA
Sbjct: 182 TGLQLPGILDELFSYDGPLGAFYLEEAVSLYLWAPTA 218