BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00014726
         (4803 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_023759388.1| eIF-2-alpha kinase GCN2 isoform X1 [Lactuca ...  1857   0.0  
ref|XP_022029921.1| eIF-2-alpha kinase GCN2 [Helianthus annuus] ...  1851   0.0  
ref|XP_023759389.1| eIF-2-alpha kinase GCN2 isoform X2 [Lactuca ...  1787   0.0  
ref|XP_023759390.1| eIF-2-alpha kinase GCN2 isoform X3 [Lactuca ...  1496   0.0  
ref|XP_022873128.1| eIF-2-alpha kinase GCN2 isoform X1 [Olea eur...  1496   0.0  
ref|XP_020409699.1| eIF-2-alpha kinase GCN2 [Prunus persica] >gi...  1484   0.0  
ref|XP_024195140.1| eIF-2-alpha kinase GCN2 isoform X2 [Rosa chi...  1483   0.0  
ref|XP_021813367.1| eIF-2-alpha kinase GCN2 isoform X1 [Prunus a...  1482   0.0  
ref|XP_016648036.1| PREDICTED: eIF-2-alpha kinase GCN2 [Prunus m...  1482   0.0  
ref|XP_024195138.1| eIF-2-alpha kinase GCN2 isoform X1 [Rosa chi...  1480   0.0  
ref|XP_021627653.1| eIF-2-alpha kinase GCN2 isoform X1 [Manihot ...  1472   0.0  
ref|XP_015901852.1| PREDICTED: eIF-2-alpha kinase GCN2 isoform X...  1470   0.0  
ref|XP_015901851.1| PREDICTED: eIF-2-alpha kinase GCN2 isoform X...  1468   0.0  
gb|AMY99278.1| serine/threonine-protein kinase GCN2 [Nicotiana t...  1466   0.0  
ref|XP_024030685.1| eIF-2-alpha kinase GCN2 isoform X2 [Morus no...  1464   0.0  
ref|XP_019253353.1| PREDICTED: eIF-2-alpha kinase GCN2 isoform X...  1461   0.0  
ref|XP_024030684.1| eIF-2-alpha kinase GCN2 isoform X1 [Morus no...  1459   0.0  
dbj|GAV69857.1| Pkinase domain-containing protein/RWD domain-con...  1457   0.0  
ref|XP_018806250.1| PREDICTED: eIF-2-alpha kinase GCN2 isoform X...  1454   0.0  
ref|NP_001311633.1| eIF-2-alpha kinase GCN2 [Nicotiana tabacum] ...  1451   0.0  

>ref|XP_023759388.1| eIF-2-alpha kinase GCN2 isoform X1 [Lactuca sativa]
          Length = 1221

 Score = 1857 bits (4810), Expect = 0.0
 Identities = 953/1212 (78%), Positives = 1030/1212 (84%), Gaps = 22/1212 (1%)
 Frame = +2

Query: 233  PATDHTSLDN---ELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVS 397
            P  DHTS+D    ELLSEE+TAL AIFQEDCDV+SE+  RIKIKLRPYSQDSGYEDLDVS
Sbjct: 19   PTNDHTSIDGVGGELLSEELTALLAIFQEDCDVVSESPPRIKIKLRPYSQDSGYEDLDVS 78

Query: 398  ALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAA 577
            ALLSIRFL GYPYKCPKL I  EKGLSKSDADNLLSLL+DQANLNAREGRVM+YNLVEAA
Sbjct: 79   ALLSIRFLPGYPYKCPKLLIIPEKGLSKSDADNLLSLLHDQANLNAREGRVMVYNLVEAA 138

Query: 578  QEFLSEVAPLEQPHLPRKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHWGLSVEEXXXXX 757
            QEFLSEV P+EQPH+P     V++DRPSKGPFVYGF+DLF+GS ESW WGLS+EE     
Sbjct: 139  QEFLSEVVPMEQPHVP----CVTTDRPSKGPFVYGFIDLFMGSGESWPWGLSIEESNNKN 194

Query: 758  XXLD-----DLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXXXXXXXXXXXXXX 922
              L+     + P+ +   + KVNQNMK  VVED  ++KV++ T +               
Sbjct: 195  TSLELHHALEDPHNTSLEKVKVNQNMKTGVVEDGDMDKVISPTSRLDDLEEESKSTNSFT 254

Query: 923  XXXXXXXXXXXVGEDYPLAXXXXXXXXXXXXXXXHSGSASAASIDHHQISHDVEKDLILA 1102
                       +GEDYPL                HS S S+AS DHHQISH VE+DLILA
Sbjct: 255  SSSEELVDNVSIGEDYPLEENTEEETDYGEIKSDHSESVSSASTDHHQISHTVERDLILA 314

Query: 1103 QLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLFDKSFNQAFGHRTVTS 1282
             LLRLAC+ KGPLADALTDV SEL +LGIVS+RVADLATESSS FD+SFNQAFGHR VTS
Sbjct: 315  HLLRLACAPKGPLADALTDVTSELVNLGIVSERVADLATESSSHFDRSFNQAFGHRMVTS 374

Query: 1283 RISQFWKTASDTKGQ-HTSPAFSSRYLNDFEELQPLGHGGFGNVVLCKNKLDGRQYAVKK 1459
            +IS FW+T SD++GQ HTSP  SSRYLNDFEELQPLGHGGFG+VVLCKNKLDGRQYAVKK
Sbjct: 375  KISHFWRTVSDSRGQQHTSPTLSSRYLNDFEELQPLGHGGFGHVVLCKNKLDGRQYAVKK 434

Query: 1460 IRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYG-DATWGSR-AAASS 1633
            IRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETG  G Y  DAT GSR AAASS
Sbjct: 435  IRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGVGGLYNNDATLGSRTAAASS 494

Query: 1634 SFSYLDQSSTDVIG--QDNNVGTYLYIQMEYCPRTLRQMFESYQ-FEKKPAWHLFRQIVE 1804
            SFS++DQSSTDV+G  QDN VGTYLYIQMEYCPRTLRQMFESY  F+KK AWHLFRQIVE
Sbjct: 495  SFSFIDQSSTDVVGVTQDNKVGTYLYIQMEYCPRTLRQMFESYSHFDKKLAWHLFRQIVE 554

Query: 1805 GLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPDPTETTGMSIDG 1984
            GLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLE D DP ETTG+S+DG
Sbjct: 555  GLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEQDVDPAETTGVSVDG 614

Query: 1985 TGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHVVLSDLKKKGEL 2164
            TGQIGTYFYTAPEIEQRWPKINEKADMYSLGI+FLE+WHPFSTGMERH+VLSDLKKKGEL
Sbjct: 615  TGQIGTYFYTAPEIEQRWPKINEKADMYSLGIVFLEIWHPFSTGMERHIVLSDLKKKGEL 674

Query: 2165 PSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNILRTMHNSEDTS 2344
            P DW  EFPEQA                ATELL+HAFPPRMESELLDN LRTMHNSEDTS
Sbjct: 675  PLDWITEFPEQASLLRRMMSSIPSDRPSATELLQHAFPPRMESELLDNFLRTMHNSEDTS 734

Query: 2345 IYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRDLVWEVSSTVFRL 2524
            IYDKVV A+FSEE ++TKN    +E+PKLG  + SSIQHTDLDTEIRDLVWEVS+ VF+L
Sbjct: 735  IYDKVVDAIFSEETLSTKNH---AEIPKLGGNDTSSIQHTDLDTEIRDLVWEVSAAVFKL 791

Query: 2525 HCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWVVLNQKSSFKRF 2704
            HCAKRLEIIPM LLGDSLQ NRN VKLLT GGDM+ELCHELRLPFINWVVLNQKSSFKRF
Sbjct: 792  HCAKRLEIIPMRLLGDSLQFNRNTVKLLTSGGDMVELCHELRLPFINWVVLNQKSSFKRF 851

Query: 2705 EICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFFLPESCDIHLNH 2884
            EICYVYRR IGHSPPNRYLQGDFDVIGGAS+LTEAEVIKAAMDIITRFF PESCDIHLNH
Sbjct: 852  EICYVYRRSIGHSPPNRYLQGDFDVIGGASSLTEAEVIKAAMDIITRFFDPESCDIHLNH 911

Query: 2885 GDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQLRQELNITEGVV 3064
            GDLLEAIWSWIGIK++HRQKVAELLSLLGSLRPQS+ERK+ WVVIRRQLRQELNI+EGVV
Sbjct: 912  GDLLEAIWSWIGIKSEHRQKVAELLSLLGSLRPQSSERKTKWVVIRRQLRQELNISEGVV 971

Query: 3065 NKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLRIWKIDKNVFIDP 3244
            NKLQ VGLRFCGIAD ALPRLRGAL    DTL  KAL+ELSQ+  YLRIW+I+KNVFIDP
Sbjct: 972  NKLQTVGLRFCGIADQALPRLRGALP--ADTLTRKALDELSQLFNYLRIWRINKNVFIDP 1029

Query: 3245 LIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQSEHKSNPPRAVG 3424
            L+PPT+ YH+DSFFQI+LRK +S+G+ TEGTLLAVGGRYDYLLH MWQSE+KSNPP AVG
Sbjct: 1030 LMPPTQAYHKDSFFQIYLRKDSSLGSLTEGTLLAVGGRYDYLLHNMWQSEYKSNPPGAVG 1089

Query: 3425 TSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAELWEDDIKAEFVP 3604
            TSLALETIIQHSSVDI RPFRNDSS+SVLVCSRGGGGLLEKRMELVAELWEDDIKAEFVP
Sbjct: 1090 TSLALETIIQHSSVDIYRPFRNDSSRSVLVCSRGGGGLLEKRMELVAELWEDDIKAEFVP 1149

Query: 3605 IVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKEVSRESLVKFLSEA 3766
            I+DPS  EQY+YA EHDIKCLI +S+      GSVKIRHLELKREKEVSRESL KFLSEA
Sbjct: 1150 ILDPSLTEQYEYANEHDIKCLIIISDTGVSQKGSVKIRHLELKREKEVSRESLTKFLSEA 1209

Query: 3767 MASQFRNPSIWN 3802
            MASQFRNPSIWN
Sbjct: 1210 MASQFRNPSIWN 1221


>ref|XP_022029921.1| eIF-2-alpha kinase GCN2 [Helianthus annuus]
 gb|OTG32846.1| putative protein kinase family protein [Helianthus annuus]
          Length = 1215

 Score = 1851 bits (4795), Expect = 0.0
 Identities = 948/1202 (78%), Positives = 1020/1202 (84%), Gaps = 12/1202 (0%)
 Frame = +2

Query: 233  PATDHTSLDN---ELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVS 397
            P +DHTS+D    ELLS+E+TALCAI QED DV+SE+  +IKIKLRPYSQDSGYED+DVS
Sbjct: 19   PPSDHTSIDGVGGELLSDELTALCAILQEDYDVVSESPPQIKIKLRPYSQDSGYEDVDVS 78

Query: 398  ALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAA 577
            A LSIRFL GYPYK PKL I  EKGLSKSDADNLLSLL+DQANLNAREGRVMIYNLVEAA
Sbjct: 79   AFLSIRFLPGYPYKYPKLTIIPEKGLSKSDADNLLSLLHDQANLNAREGRVMIYNLVEAA 138

Query: 578  QEFLSEVAPLEQPHLPRKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHWGLSVEEXXXXX 757
            QEFLSEV  ++QPHL RK   VS DRPSKGPFVYGFLDLFIGS ESWHWGLSVEE     
Sbjct: 139  QEFLSEVVTIDQPHLSRKNATVSCDRPSKGPFVYGFLDLFIGSGESWHWGLSVEESNSTI 198

Query: 758  XXLDDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXXXXXXXXXXXXXXXXXXX 937
              LD+L NG+QPS +KVN  +KM V+E  +L KV ++                       
Sbjct: 199  VALDNLNNGAQPSWEKVNPTLKMGVIEGGQL-KVKSTERLDILEEDDSKSTDSLASQSAE 257

Query: 938  XXXXXXVGEDYPLAXXXXXXXXXXXXXXXHSGSASAASIDHHQISHDVEKDLILAQLLRL 1117
                  VGEDYPLA                  SAS  S+DHHQISH VE+DLILA LLRL
Sbjct: 258  PVENVSVGEDYPLAEDTEEETDYDETESDILESAS--SVDHHQISHTVERDLILAHLLRL 315

Query: 1118 ACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLFDKSFNQAFGHRTVTSRISQF 1297
            AC+ KGPLADALTDV SEL +LGIVSDRVADLAT SSSLFD+SFNQAFG+R V S+IS F
Sbjct: 316  ACAPKGPLADALTDVTSELVNLGIVSDRVADLATGSSSLFDRSFNQAFGNRMVASKISNF 375

Query: 1298 WKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVVLCKNKLDGRQYAVKKIRLKDK 1477
            WKTA+D+KGQHTSP  SSRYLNDFEELQP+GHGGFG+VVLCKNKLDGRQYAVKKIRLKDK
Sbjct: 376  WKTAADSKGQHTSPTLSSRYLNDFEELQPIGHGGFGHVVLCKNKLDGRQYAVKKIRLKDK 435

Query: 1478 SQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYGDATWGSRAAASSSFSYLDQS 1657
            SQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAG Y DATWGSR AASSS+SY+D +
Sbjct: 436  SQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGFYDDATWGSRTAASSSYSYIDHT 495

Query: 1658 STDVIGQDNNVGTYLYIQMEYCPRTLRQMFESYQ-FEKKPAWHLFRQIVEGLAHIHSQGI 1834
            STDVIGQDN VGTYLYIQMEYCPRTLRQMFESY  F+KK AWHLFRQIVEGLAHIHSQGI
Sbjct: 496  STDVIGQDNKVGTYLYIQMEYCPRTLRQMFESYSHFDKKDAWHLFRQIVEGLAHIHSQGI 555

Query: 1835 IHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPDPTETTGMSIDGTGQIGTYFYT 2014
            IHRDLTPNNIFFDAR+DIKIGDFGLAKFLKLEQL+ D DP ETTG+S+DGTGQIGTYFYT
Sbjct: 556  IHRDLTPNNIFFDARSDIKIGDFGLAKFLKLEQLDQDVDPAETTGVSVDGTGQIGTYFYT 615

Query: 2015 APEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHVVLSDLKKKGELPSDWAAEFPE 2194
            APEIEQRWPKINEKADMYSLGI+F ELWHPFSTGMERH+VLSDLKKKGELPSDW AEFPE
Sbjct: 616  APEIEQRWPKINEKADMYSLGIVFFELWHPFSTGMERHIVLSDLKKKGELPSDWVAEFPE 675

Query: 2195 QAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNILRTMHNSEDTSIYDKVVGAVF 2374
            QA                A ELL+HA PPRMESELLDNILRTMHNSEDTSIYDKVV A+F
Sbjct: 676  QAALLRRMMSSSPSDRPSANELLQHACPPRMESELLDNILRTMHNSEDTSIYDKVVNAIF 735

Query: 2375 SEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRDLVWEVSSTVFRLHCAKRLEIIP 2554
            SEEI++TK QDN++E  KLG    SSIQHTDLDTEIRDLV EVS+ VFRLHCAK LE IP
Sbjct: 736  SEEILSTKIQDNDTETLKLGRNVTSSIQHTDLDTEIRDLVSEVSAAVFRLHCAKHLETIP 795

Query: 2555 MCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWVVLNQKSSFKRFEICYVYRRPI 2734
            M LLGDS+Q NRN VKLLT+GGDM+ELCHELRLPFINWVVLNQKS+FKRFEI YVYRR I
Sbjct: 796  MRLLGDSVQFNRNNVKLLTNGGDMLELCHELRLPFINWVVLNQKSTFKRFEIGYVYRRSI 855

Query: 2735 GHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFFLPESCDIHLNHGDLLEAIWSW 2914
            GHSPPNRYLQGDFDVIGG SA+TEAEVIKA MDIITRFF PESCDIHLNHGDLLEAIWSW
Sbjct: 856  GHSPPNRYLQGDFDVIGGDSAITEAEVIKATMDIITRFFHPESCDIHLNHGDLLEAIWSW 915

Query: 2915 IGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQLRQELNITEGVVNKLQNVGLRF 3094
            IGIKADHRQKVAELLSLLGSLRPQSTERK+ WVVIRRQLRQELNITEG VNKLQ VGLRF
Sbjct: 916  IGIKADHRQKVAELLSLLGSLRPQSTERKTKWVVIRRQLRQELNITEGAVNKLQTVGLRF 975

Query: 3095 CGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLRIWKIDKNVFIDPLIPPTEGYHR 3274
            CG ADHALPRLRGAL    DTL  KAL+ELSQ+  YLRIWKIDK+VFIDPL+PPTEGYHR
Sbjct: 976  CGSADHALPRLRGALPS--DTLTRKALDELSQLFNYLRIWKIDKHVFIDPLMPPTEGYHR 1033

Query: 3275 DSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQSEHKSNPPRAVGTSLALETIIQ 3454
            DSFFQI+LRK N++G+ TEGTLLAVGGRYDYLLH+MWQSE+KSNPP AVGTSLALETII 
Sbjct: 1034 DSFFQIYLRKDNTLGSHTEGTLLAVGGRYDYLLHRMWQSEYKSNPPGAVGTSLALETIIH 1093

Query: 3455 HSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAELWEDDIKAEFVPIVDPSPKEQY 3634
            HSSVD+ RPFRNDSS+SVLVCSRGGGGLLEKRMELV EL EDDIKAEFVP +DPS  EQY
Sbjct: 1094 HSSVDVYRPFRNDSSRSVLVCSRGGGGLLEKRMELVTELREDDIKAEFVPTLDPSLTEQY 1153

Query: 3635 DYAKEHDIKCLIEMSE------GSVKIRHLELKREKEVSRESLVKFLSEAMASQFRNPSI 3796
            +YA E+DIKCLI +S+      GSVKIRHLELK+EKEVSRESL  FLS+AMASQFRNPSI
Sbjct: 1154 EYANENDIKCLIIISDTGVSQNGSVKIRHLELKKEKEVSRESLANFLSDAMASQFRNPSI 1213

Query: 3797 WN 3802
            WN
Sbjct: 1214 WN 1215


>ref|XP_023759389.1| eIF-2-alpha kinase GCN2 isoform X2 [Lactuca sativa]
          Length = 1194

 Score = 1787 bits (4629), Expect = 0.0
 Identities = 927/1212 (76%), Positives = 1004/1212 (82%), Gaps = 22/1212 (1%)
 Frame = +2

Query: 233  PATDHTSLDN---ELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVS 397
            P  DHTS+D    ELLSEE+TAL AIFQEDCDV+SE+  RIKIKLRPYSQDSGYEDLDVS
Sbjct: 19   PTNDHTSIDGVGGELLSEELTALLAIFQEDCDVVSESPPRIKIKLRPYSQDSGYEDLDVS 78

Query: 398  ALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAA 577
            ALLSIRFL GYPYKCPKL I  EKGLSKSDADNLLSLL+DQANLNAREGRVM+YNLVEAA
Sbjct: 79   ALLSIRFLPGYPYKCPKLLIIPEKGLSKSDADNLLSLLHDQANLNAREGRVMVYNLVEAA 138

Query: 578  QEFLSEVAPLEQPHLPRKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHWGLSVEEXXXXX 757
            QEFLSEV P+EQPH+P     V++DRPSKGPFVYGF+DLF+GS ESW WGLS+EE     
Sbjct: 139  QEFLSEVVPMEQPHVP----CVTTDRPSKGPFVYGFIDLFMGSGESWPWGLSIEESNNKN 194

Query: 758  XXLD-----DLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXXXXXXXXXXXXXX 922
              L+     + P+ +   + KVNQNMK  VVED  ++KV++ T +               
Sbjct: 195  TSLELHHALEDPHNTSLEKVKVNQNMKTGVVEDGDMDKVISPTSRLDDLEEESKSTNSFT 254

Query: 923  XXXXXXXXXXXVGEDYPLAXXXXXXXXXXXXXXXHSGSASAASIDHHQISHDVEKDLILA 1102
                       +GEDYPL                HS S S+AS DHHQISH VE+DLILA
Sbjct: 255  SSSEELVDNVSIGEDYPLEENTEEETDYGEIKSDHSESVSSASTDHHQISHTVERDLILA 314

Query: 1103 QLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLFDKSFNQAFGHRTVTS 1282
             LLRLAC+ KGPLADALTDV SEL +LGIVS+RVADLATESSS FD+SFNQAFGHR VTS
Sbjct: 315  HLLRLACAPKGPLADALTDVTSELVNLGIVSERVADLATESSSHFDRSFNQAFGHRMVTS 374

Query: 1283 RISQFWKTASDTKGQ-HTSPAFSSRYLNDFEELQPLGHGGFGNVVLCKNKLDGRQYAVKK 1459
            +IS FW+T SD++GQ HTSP  SSRYLNDFEELQPLGHGGFG+VVLCKNKLDGRQYAVKK
Sbjct: 375  KISHFWRTVSDSRGQQHTSPTLSSRYLNDFEELQPLGHGGFGHVVLCKNKLDGRQYAVKK 434

Query: 1460 IRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYG-DATWGSR-AAASS 1633
            IRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETG  G Y  DAT GSR AAASS
Sbjct: 435  IRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGVGGLYNNDATLGSRTAAASS 494

Query: 1634 SFSYLDQSSTDVIG--QDNNVGTYLYIQMEYCPRTLRQMFESYQ-FEKKPAWHLFRQIVE 1804
            SFS++DQSSTDV+G  QDN VGTYLYIQMEYCPRTLRQMFESY  F+KK AWHLFRQIVE
Sbjct: 495  SFSFIDQSSTDVVGVTQDNKVGTYLYIQMEYCPRTLRQMFESYSHFDKKLAWHLFRQIVE 554

Query: 1805 GLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPDPTETTGMSIDG 1984
            GLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLE D DP ETTG+S+DG
Sbjct: 555  GLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEQDVDPAETTGVSVDG 614

Query: 1985 TGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHVVLSDLKKKGEL 2164
            TGQIGTYFYTAPEIEQRWPKINEKADMYSLGI+FLE+WHPFSTGMERH+VLSDLKKKGEL
Sbjct: 615  TGQIGTYFYTAPEIEQRWPKINEKADMYSLGIVFLEIWHPFSTGMERHIVLSDLKKKGEL 674

Query: 2165 PSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNILRTMHNSEDTS 2344
            P DW  EFPEQA                ATELL+HAFPPRMESELLDN LRTMHNSEDTS
Sbjct: 675  PLDWITEFPEQASLLRRMMSSIPSDRPSATELLQHAFPPRMESELLDNFLRTMHNSEDTS 734

Query: 2345 IYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRDLVWEVSSTVFRL 2524
            IYDKVV A+FSEE ++TKN    +E+PKLG  + SSIQHTDLDTEIRDLVWEVS+ VF+L
Sbjct: 735  IYDKVVDAIFSEETLSTKNH---AEIPKLGGNDTSSIQHTDLDTEIRDLVWEVSAAVFKL 791

Query: 2525 HCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWVVLNQKSSFKRF 2704
            HCAKRLEIIPM LLGDSLQ NRN VKLLT GGDM+ELCHELRLPFINWVVLNQ       
Sbjct: 792  HCAKRLEIIPMRLLGDSLQFNRNTVKLLTSGGDMVELCHELRLPFINWVVLNQ------- 844

Query: 2705 EICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFFLPESCDIHLNH 2884
                                GDFDVIGGAS+LTEAEVIKAAMDIITRFF PESCDIHLNH
Sbjct: 845  --------------------GDFDVIGGASSLTEAEVIKAAMDIITRFFDPESCDIHLNH 884

Query: 2885 GDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQLRQELNITEGVV 3064
            GDLLEAIWSWIGIK++HRQKVAELLSLLGSLRPQS+ERK+ WVVIRRQLRQELNI+EGVV
Sbjct: 885  GDLLEAIWSWIGIKSEHRQKVAELLSLLGSLRPQSSERKTKWVVIRRQLRQELNISEGVV 944

Query: 3065 NKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLRIWKIDKNVFIDP 3244
            NKLQ VGLRFCGIAD ALPRLRGAL    DTL  KAL+ELSQ+  YLRIW+I+KNVFIDP
Sbjct: 945  NKLQTVGLRFCGIADQALPRLRGALPA--DTLTRKALDELSQLFNYLRIWRINKNVFIDP 1002

Query: 3245 LIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQSEHKSNPPRAVG 3424
            L+PPT+ YH+DSFFQI+LRK +S+G+ TEGTLLAVGGRYDYLLH MWQSE+KSNPP AVG
Sbjct: 1003 LMPPTQAYHKDSFFQIYLRKDSSLGSLTEGTLLAVGGRYDYLLHNMWQSEYKSNPPGAVG 1062

Query: 3425 TSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAELWEDDIKAEFVP 3604
            TSLALETIIQHSSVDI RPFRNDSS+SVLVCSRGGGGLLEKRMELVAELWEDDIKAEFVP
Sbjct: 1063 TSLALETIIQHSSVDIYRPFRNDSSRSVLVCSRGGGGLLEKRMELVAELWEDDIKAEFVP 1122

Query: 3605 IVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKEVSRESLVKFLSEA 3766
            I+DPS  EQY+YA EHDIKCLI +S+      GSVKIRHLELKREKEVSRESL KFLSEA
Sbjct: 1123 ILDPSLTEQYEYANEHDIKCLIIISDTGVSQKGSVKIRHLELKREKEVSRESLTKFLSEA 1182

Query: 3767 MASQFRNPSIWN 3802
            MASQFRNPSIWN
Sbjct: 1183 MASQFRNPSIWN 1194


>ref|XP_023759390.1| eIF-2-alpha kinase GCN2 isoform X3 [Lactuca sativa]
 gb|PLY88842.1| hypothetical protein LSAT_3X115420 [Lactuca sativa]
          Length = 1048

 Score = 1496 bits (3873), Expect = 0.0
 Identities = 768/985 (77%), Positives = 828/985 (84%), Gaps = 16/985 (1%)
 Frame = +2

Query: 233  PATDHTSLDN---ELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVS 397
            P  DHTS+D    ELLSEE+TAL AIFQEDCDV+SE+  RIKIKLRPYSQDSGYEDLDVS
Sbjct: 19   PTNDHTSIDGVGGELLSEELTALLAIFQEDCDVVSESPPRIKIKLRPYSQDSGYEDLDVS 78

Query: 398  ALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAA 577
            ALLSIRFL GYPYKCPKL I  EKGLSKSDADNLLSLL+DQANLNAREGRVM+YNLVEAA
Sbjct: 79   ALLSIRFLPGYPYKCPKLLIIPEKGLSKSDADNLLSLLHDQANLNAREGRVMVYNLVEAA 138

Query: 578  QEFLSEVAPLEQPHLPRKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHWGLSVEEXXXXX 757
            QEFLSEV P+EQPH+P     V++DRPSKGPFVYGF+DLF+GS ESW WGLS+EE     
Sbjct: 139  QEFLSEVVPMEQPHVP----CVTTDRPSKGPFVYGFIDLFMGSGESWPWGLSIEESNNKN 194

Query: 758  XXLD-----DLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXXXXXXXXXXXXXX 922
              L+     + P+ +   + KVNQNMK  VVED  ++KV++ T +               
Sbjct: 195  TSLELHHALEDPHNTSLEKVKVNQNMKTGVVEDGDMDKVISPTSRLDDLEEESKSTNSFT 254

Query: 923  XXXXXXXXXXXVGEDYPLAXXXXXXXXXXXXXXXHSGSASAASIDHHQISHDVEKDLILA 1102
                       +GEDYPL                HS S S+AS DHHQISH VE+DLILA
Sbjct: 255  SSSEELVDNVSIGEDYPLEENTEEETDYGEIKSDHSESVSSASTDHHQISHTVERDLILA 314

Query: 1103 QLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLFDKSFNQAFGHRTVTS 1282
             LLRLAC+ KGPLADALTDV SEL +LGIVS+RVADLATESSS FD+SFNQAFGHR VTS
Sbjct: 315  HLLRLACAPKGPLADALTDVTSELVNLGIVSERVADLATESSSHFDRSFNQAFGHRMVTS 374

Query: 1283 RISQFWKTASDTKGQ-HTSPAFSSRYLNDFEELQPLGHGGFGNVVLCKNKLDGRQYAVKK 1459
            +IS FW+T SD++GQ HTSP  SSRYLNDFEELQPLGHGGFG+VVLCKNKLDGRQYAVKK
Sbjct: 375  KISHFWRTVSDSRGQQHTSPTLSSRYLNDFEELQPLGHGGFGHVVLCKNKLDGRQYAVKK 434

Query: 1460 IRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYG-DATWGSR-AAASS 1633
            IRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETG  G Y  DAT GSR AAASS
Sbjct: 435  IRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGVGGLYNNDATLGSRTAAASS 494

Query: 1634 SFSYLDQSSTDVIG--QDNNVGTYLYIQMEYCPRTLRQMFESYQ-FEKKPAWHLFRQIVE 1804
            SFS++DQSSTDV+G  QDN VGTYLYIQMEYCPRTLRQMFESY  F+KK AWHLFRQIVE
Sbjct: 495  SFSFIDQSSTDVVGVTQDNKVGTYLYIQMEYCPRTLRQMFESYSHFDKKLAWHLFRQIVE 554

Query: 1805 GLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPDPTETTGMSIDG 1984
            GLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLE D DP ETTG+S+DG
Sbjct: 555  GLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEQDVDPAETTGVSVDG 614

Query: 1985 TGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHVVLSDLKKKGEL 2164
            TGQIGTYFYTAPEIEQRWPKINEKADMYSLGI+FLE+WHPFSTGMERH+VLSDLKKKGEL
Sbjct: 615  TGQIGTYFYTAPEIEQRWPKINEKADMYSLGIVFLEIWHPFSTGMERHIVLSDLKKKGEL 674

Query: 2165 PSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNILRTMHNSEDTS 2344
            P DW  EFPEQA                ATELL+HAFPPRMESELLDN LRTMHNSEDTS
Sbjct: 675  PLDWITEFPEQASLLRRMMSSIPSDRPSATELLQHAFPPRMESELLDNFLRTMHNSEDTS 734

Query: 2345 IYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRDLVWEVSSTVFRL 2524
            IYDKVV A+FSEE ++TKN    +E+PKLG  + SSIQHTDLDTEIRDLVWEVS+ VF+L
Sbjct: 735  IYDKVVDAIFSEETLSTKNH---AEIPKLGGNDTSSIQHTDLDTEIRDLVWEVSAAVFKL 791

Query: 2525 HCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWVVLNQKSSFKRF 2704
            HCAKRLEIIPM LLGDSLQ NRN VKLLT GGDM+ELCHELRLPFINWVVLNQKSSFKRF
Sbjct: 792  HCAKRLEIIPMRLLGDSLQFNRNTVKLLTSGGDMVELCHELRLPFINWVVLNQKSSFKRF 851

Query: 2705 EICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFFLPESCDIHLNH 2884
            EICYVYRR IGHSPPNRYLQGDFDVIGGAS+LTEAEVIKAAMDIITRFF PESCDIHLNH
Sbjct: 852  EICYVYRRSIGHSPPNRYLQGDFDVIGGASSLTEAEVIKAAMDIITRFFDPESCDIHLNH 911

Query: 2885 GDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQLRQELNITEGVV 3064
            GDLLEAIWSWIGIK++HRQKVAELLSLLGSLRPQS+ERK+ WVVIRRQLRQELNI+EGVV
Sbjct: 912  GDLLEAIWSWIGIKSEHRQKVAELLSLLGSLRPQSSERKTKWVVIRRQLRQELNISEGVV 971

Query: 3065 NKLQNVGLRFCGIADHALPRLRGAL 3139
            NKLQ VGLRFCGIAD ALPRLRGAL
Sbjct: 972  NKLQTVGLRFCGIADQALPRLRGAL 996


>ref|XP_022873128.1| eIF-2-alpha kinase GCN2 isoform X1 [Olea europaea var. sylvestris]
          Length = 1238

 Score = 1496 bits (3873), Expect = 0.0
 Identities = 783/1223 (64%), Positives = 917/1223 (74%), Gaps = 34/1223 (2%)
 Frame = +2

Query: 236  ATDHTSL---DNELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVSA 400
            A D  SL   ++ELLSEE+TALCAIFQEDC+V+SE+  +I IKLRPYS+D+GYED DVSA
Sbjct: 21   AKDQNSLAADNSELLSEELTALCAIFQEDCEVVSESPPQINIKLRPYSKDTGYEDSDVSA 80

Query: 401  LLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQ 580
            LLS+RFL GYPYK PKLQI  EKGLSKSDADNLLSLL+DQA+LNAREGRVMIYNLVEAAQ
Sbjct: 81   LLSVRFLHGYPYKSPKLQIVPEKGLSKSDADNLLSLLHDQASLNAREGRVMIYNLVEAAQ 140

Query: 581  EFLSEVAPLEQPH-------------LPRKTVAVSSDRPS--KGPFVYGFLDLFIGSEES 715
            EFLSEV    Q               L +K   +SS + +  +GPF+Y  +DLF GS ES
Sbjct: 141  EFLSEVVSQGQSQESVRCQETDNKGQLSQKNATISSSKINSFRGPFIYSHVDLFSGSGES 200

Query: 716  WHWGLSVEEXXXXXXX-LDDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXXXX 892
            W+W L +E+          +       S DKV QN+K   VE+ K   +   + +     
Sbjct: 201  WNWNLGMEDSTQIVHQHTSEGSKQGNDSLDKVEQNVKPATVENLKTESLHKPSSRLDPLE 260

Query: 893  XXXXXXXXXXXXXXXXXXXXXVGEDYP------LAXXXXXXXXXXXXXXXHSGSASAASI 1054
                                  G D P                        S   S  S+
Sbjct: 261  EESEDETRSTNSSGKISDESD-GNDVPGDFKDIFVVGNQIETDYEDVYGESSEPLSFESV 319

Query: 1055 DHHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSL 1234
             H Q S  V +DLILA LLR+AC+SKGP ADAL ++ SEL +LGIVS+ V DLA + S++
Sbjct: 320  AHDQPSQPVARDLILAHLLRIACASKGPFADALPEITSELLNLGIVSETVRDLAVKPSAV 379

Query: 1235 FDKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVV 1414
            FDK+ N  FG   V+S+IS+FW  ASD  G+H+S   SSRYLNDFEELQPLGHGGFG+VV
Sbjct: 380  FDKTLNCIFGKHIVSSKISKFWTMASDF-GEHSSSIPSSRYLNDFEELQPLGHGGFGHVV 438

Query: 1415 LCKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCY 1594
            LCKNKLDGRQYAVKKIRLKDKS P++DRILREVATLSRLQHQHVVRYYQAWFETG  G +
Sbjct: 439  LCKNKLDGRQYAVKKIRLKDKSLPVNDRILREVATLSRLQHQHVVRYYQAWFETGIVGSH 498

Query: 1595 GDATWGSRAAASSSFSYLDQSSTDVIGQDNNVGTYLYIQMEYCPRTLRQMFESYQ-FEKK 1771
            GDA WGS+   SSSFSY + SS++   Q+    TYLYIQMEYCPRTLRQMFESY   +K+
Sbjct: 499  GDAFWGSKTGLSSSFSYDNTSSSEFGPQNKLDSTYLYIQMEYCPRTLRQMFESYNNLDKE 558

Query: 1772 PAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPD 1951
             AWHLFRQIVEGL+HIH QGIIHRDLTPNNIFF ARNDIKIGDFGLAKFLKLEQL+ D D
Sbjct: 559  LAWHLFRQIVEGLSHIHGQGIIHRDLTPNNIFFGARNDIKIGDFGLAKFLKLEQLDQDVD 618

Query: 1952 PTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHV 2131
              ET G+S+D TGQ+GTYFYTAPEIEQ WPKI+EKADMYSL ++F ELWHPF T MERHV
Sbjct: 619  AGETVGVSVDRTGQVGTYFYTAPEIEQGWPKIDEKADMYSLAVVFFELWHPFDTAMERHV 678

Query: 2132 VLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNI 2311
            VLSDLK+KGELP++W AEFPEQA                ATELL+HAFPPRME ++LDNI
Sbjct: 679  VLSDLKQKGELPANWVAEFPEQASLLRRLMSASPSDRPSATELLQHAFPPRMEYDMLDNI 738

Query: 2312 LRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRDL 2491
            LRT+H+SEDTSIY+K+V A+F E+++++K        PKL  ++ SSI  TD+ +  RD 
Sbjct: 739  LRTIHSSEDTSIYEKLVNAIFDEDMLSSKGIQESVGRPKLVGQDTSSILFTDVCSANRDQ 798

Query: 2492 VWEVSSTVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWV 2671
            V EV++ VFR HCAK LEIIPM +L + LQLNRN VKLLT GGDM+ELCHELR PF+ W 
Sbjct: 799  VVEVATEVFRKHCAKHLEIIPMRMLDERLQLNRNTVKLLTPGGDMVELCHELRFPFVKWA 858

Query: 2672 VLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFF 2851
            + NQKS FKR+EI YVYRR IGHSPPNRYLQGDFD+IGGA+ LTEAEVIKA +DI+TRFF
Sbjct: 859  ITNQKSFFKRYEISYVYRRAIGHSPPNRYLQGDFDIIGGAATLTEAEVIKATVDIVTRFF 918

Query: 2852 LPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQL 3031
              ESCDIHLNHGDLLEAIWSW GIK +HRQKV++LLSLLGSLRPQS+ERKS WVVIRRQL
Sbjct: 919  QSESCDIHLNHGDLLEAIWSWTGIKPEHRQKVSDLLSLLGSLRPQSSERKSKWVVIRRQL 978

Query: 3032 RQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLRI 3211
            RQELN++E  VN+LQ VGLRFCGIADHALPRLRGAL    D    KAL+ELS++L YLR+
Sbjct: 979  RQELNLSEATVNRLQTVGLRFCGIADHALPRLRGALP--ADKSTHKALDELSELLNYLRV 1036

Query: 3212 WKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQS 3391
            W+IDK +++D L+PPTE YHR+ +FQI+LRK NS  +  EGTLLAVGGRYDYL+H M  S
Sbjct: 1037 WRIDKRLYVDALMPPTESYHRNLYFQIYLRKDNSTVSLMEGTLLAVGGRYDYLVHHMSDS 1096

Query: 3392 EHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAEL 3571
            EHK NPP AVGTSLALETI+ HSSVDI +P RND   +VLVCSRGGGGLL +RMELVAEL
Sbjct: 1097 EHKLNPPGAVGTSLALETILMHSSVDI-KPHRNDVGINVLVCSRGGGGLLVERMELVAEL 1155

Query: 3572 WEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKEVS 3733
            WE++IKAE VP+ DPS  EQY+YA EHDIKCL+ +++      GSVK+RHLELK+EKEV 
Sbjct: 1156 WEENIKAELVPLCDPSLTEQYEYASEHDIKCLVIVTDTGVTQKGSVKVRHLELKKEKEVE 1215

Query: 3734 RESLVKFLSEAMASQFRNPSIWN 3802
            RE+LVKFL +AM +QFRN SIWN
Sbjct: 1216 RETLVKFLLDAMETQFRNLSIWN 1238


>ref|XP_020409699.1| eIF-2-alpha kinase GCN2 [Prunus persica]
 gb|ONI29344.1| hypothetical protein PRUPE_1G194100 [Prunus persica]
          Length = 1243

 Score = 1484 bits (3842), Expect = 0.0
 Identities = 774/1219 (63%), Positives = 926/1219 (75%), Gaps = 37/1219 (3%)
 Frame = +2

Query: 257  DNELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVSALLSIRFLDGY 430
            DNELLSEEITALCAIFQ+DC V+S +  +I IKLRP+S+D GYEDLDVSALL +R L GY
Sbjct: 33   DNELLSEEITALCAIFQDDCKVMSGSHPQIIIKLRPHSKDMGYEDLDVSALLLVRCLPGY 92

Query: 431  PYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQEFLSEVAPLE 610
            PYKCPKLQI  EKGLS+SDAD LLSL++DQAN NAREGRVMI+NLVE AQEFLSEV P+ 
Sbjct: 93   PYKCPKLQITPEKGLSQSDADRLLSLIHDQANSNAREGRVMIFNLVETAQEFLSEVVPVS 152

Query: 611  QPH-------------LPRKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHWGLSVEEXXX 751
            Q H             L +K +A+SS++  KGPFVYGF+DLF GS ESW+WG  V+E   
Sbjct: 153  QSHGSVICPTTGSSAQLFQKDIAISSNK--KGPFVYGFIDLFSGSGESWNWGFGVDETSG 210

Query: 752  XXXXLDD-LPNGSQPSRD----KVNQNMKMMVVEDNKLNKVLTSTGKXXXXXXXXXXXXX 916
                +     +GS+   +    K++++ + + ++D K + +L+ST K             
Sbjct: 211  INPSVPSHTGDGSKVKHEIQEKKLDRHAEPLNLQDIKKSSLLSSTVKLDSLEEDSEDGNK 270

Query: 917  XXXXXXXXXXXXX--VG-----EDYPLAXXXXXXXXXXXXXXXHSGSASAASIDHHQISH 1075
                           VG     E   L                 S S S AS+ H Q+S 
Sbjct: 271  SIASTNSSRFLLEELVGSGGKAEKENLVLEEDSTEDDCEFGSEQSESLSFASLGHDQVSQ 330

Query: 1076 DVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLFDKSFNQ 1255
             V+KDLI+  LLRLAC+SKGPLADAL  + +EL +LGI+S+   DLA++  SL +++FN 
Sbjct: 331  TVKKDLIMVHLLRLACTSKGPLADALPQITTELENLGILSEWGRDLASKPPSLLNRTFNH 390

Query: 1256 AFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVVLCKNKLD 1435
            AF    V+SR+SQFW+  SD +G  TS   SSRYL+DFEELQ LGHGGFG+VVLCKNKLD
Sbjct: 391  AFREHMVSSRVSQFWEPTSDFEGPSTSLP-SSRYLSDFEELQSLGHGGFGHVVLCKNKLD 449

Query: 1436 GRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYGDATWGS 1615
            GRQYAVKKIRLKDKS P++DRILREVATLSRLQHQHVVRYYQAWFETG  G +GD TWGS
Sbjct: 450  GRQYAVKKIRLKDKSLPVNDRILREVATLSRLQHQHVVRYYQAWFETGIVGAHGDTTWGS 509

Query: 1616 RAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMFESY-QFEKKPAWHLF 1789
              AASS+FS+   +S D +G +N +  TYLYIQMEYCPRTLRQ+FESY +F+K+ AWHL 
Sbjct: 510  MTAASSTFSFKGTNSADALGHENKLESTYLYIQMEYCPRTLRQVFESYSRFDKELAWHLC 569

Query: 1790 RQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPD-PTETT 1966
            RQIVEGLAHIH QGIIHRDLTP+NIFFDARNDIKIGDFGLAKFLKLEQL+ +P  P +T 
Sbjct: 570  RQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQEPSFPPDTA 629

Query: 1967 GMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHVVLSDL 2146
            G+S+DGTGQ+GTYFYTAPEIEQ WPKI+EKADMYSLG++F ELWHPF T MERH VLSDL
Sbjct: 630  GVSLDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGVVFFELWHPFGTAMERHHVLSDL 689

Query: 2147 KKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNILRTMH 2326
            K+KGELP  W AEFPEQA                ATELLKHAFPPRMESELLDNILRTM 
Sbjct: 690  KQKGELPPAWVAEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRTMQ 749

Query: 2327 NSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKEN-SSIQHTDLDTEIRDLVWEV 2503
             SED S+YDKV+ A+F EE+++ K+Q +     +LG+  + S+IQ+ DL TE RD V ++
Sbjct: 750  TSEDRSVYDKVLNAIFDEEMLSMKDQQHHDG--RLGSVSDISAIQYADLHTEARDYVVDI 807

Query: 2504 SSTVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWVVLNQ 2683
            +  VFR HCAK LE+I M LL D  Q NRN VKLLT GGDM+ELCHELRLPF++WVV +Q
Sbjct: 808  TREVFRQHCAKHLEVITMRLLDDCQQFNRNTVKLLTHGGDMLELCHELRLPFVSWVVSSQ 867

Query: 2684 KSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFFLPES 2863
            KSSFKR+E+ YV+RRP+GHSPP+RYLQGDFD+IGGASALTEAEVIK   DI+  FF  + 
Sbjct: 868  KSSFKRYEVSYVHRRPVGHSPPSRYLQGDFDIIGGASALTEAEVIKVTRDIVAPFFNSDF 927

Query: 2864 CDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQLRQEL 3043
            CDIHLNHGDLLEAIWSW+G+K++HRQKVAELLS++GSLRPQS+ERKS WVVIRRQL QEL
Sbjct: 928  CDIHLNHGDLLEAIWSWVGVKSEHRQKVAELLSMMGSLRPQSSERKSKWVVIRRQLLQEL 987

Query: 3044 NITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLRIWKID 3223
            N+ E VVN+LQ VGLRFCG AD AL RLRGAL    D    KAL+ELS +  +LR+W+I+
Sbjct: 988  NLPEAVVNRLQTVGLRFCGAADQALARLRGALP--TDKPTRKALDELSDLYSHLRVWRIE 1045

Query: 3224 KNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQSEHKS 3403
            ++V+I+PL+PPTEGYHRD FFQ++L K N+ G+ TEGTLLAVGGRYDYLL QMW  EHKS
Sbjct: 1046 RHVYINPLMPPTEGYHRDLFFQVYLVKDNNPGSLTEGTLLAVGGRYDYLLRQMWGLEHKS 1105

Query: 3404 NPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAELWEDD 3583
            +PP AVG SLALETIIQHS VD+ +P R + S  VLVCS+GGGGLL +RMELVAELWE++
Sbjct: 1106 SPPGAVGASLALETIIQHSPVDV-KPIRYEVSNDVLVCSKGGGGLLAERMELVAELWEEN 1164

Query: 3584 IKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKEVSRESL 3745
            IKAEFVPI DPS  EQY+YA EHDIKCL+ +++      GSVK+RHLELK+EKEV R +L
Sbjct: 1165 IKAEFVPIPDPSLTEQYEYANEHDIKCLVIITDTGVSQKGSVKVRHLELKKEKEVERSNL 1224

Query: 3746 VKFLSEAMASQFRNPSIWN 3802
            V+FL +AMA QF+NPSIWN
Sbjct: 1225 VRFLLDAMAIQFKNPSIWN 1243


>ref|XP_024195140.1| eIF-2-alpha kinase GCN2 isoform X2 [Rosa chinensis]
          Length = 1235

 Score = 1483 bits (3838), Expect = 0.0
 Identities = 778/1224 (63%), Positives = 913/1224 (74%), Gaps = 35/1224 (2%)
 Frame = +2

Query: 233  PATDHT-SLDNELLSEEITALCAIFQEDCDVIS--ENRIKIKLRPYSQDSGYEDLDVSAL 403
            P  DH  + DN+LLSEEITALCAIFQ+DC + S  + +I IKLRP+S+D GYEDLDVSAL
Sbjct: 22   PLKDHAGAYDNDLLSEEITALCAIFQDDCKIASGAQPQITIKLRPHSKDMGYEDLDVSAL 81

Query: 404  LSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQE 583
            L +R L GYPYKCPKLQI  EKGLSKSD+D LLSL+ DQAN NAREGRVMI+NLVEAAQE
Sbjct: 82   LLVRCLPGYPYKCPKLQITPEKGLSKSDSDKLLSLINDQANSNAREGRVMIFNLVEAAQE 141

Query: 584  FLSEVAPLEQPHLP-------------RKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHW 724
            FLSE+ P+ Q   P             +   A+SS++  KGPFVYGF+DLF GS +SW W
Sbjct: 142  FLSEIVPVGQSQGPVICSTMDNSAQLFQNDTAISSNK--KGPFVYGFIDLFSGSGKSWSW 199

Query: 725  GLSVEEXXXXXXXLDDLP--NGS-------QPSRDKVNQNMKMMVVEDNKLNKVLTSTGK 877
               V+E       +  LP  +GS       +   DK  + +K    +D K   +L+   K
Sbjct: 200  SFGVDETKGINSTVP-LPRVDGSTLMHEIQEKKHDKPAEALKS---QDIKQGPILSPNVK 255

Query: 878  XXXXXXXXXXXXXXXXXXXXXXXXXXVGEDYPLAXXXXXXXXXXXXXXXHSGSASAASID 1057
                                        E                     S S S  S+ 
Sbjct: 256  LETLEEESEGRDSSRFLLEEMDRNGGETEKESSVPEEDSTEDDWEFGSQQSDSLSFTSLA 315

Query: 1058 HHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLF 1237
            H Q S +V++DLI+  LLRLAC+ KGPLADAL  + +EL ++G++S+   DLA++  SL 
Sbjct: 316  HEQGSQNVKRDLIMVHLLRLACT-KGPLADALPQITTELQNIGVLSEWARDLASKPISLL 374

Query: 1238 DKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVVL 1417
            +++FN AFG + V+SRISQFW+  SD +G  TS   SSRYLNDFEELQ LGHGGF +VVL
Sbjct: 375  NRTFNHAFGQQMVSSRISQFWELTSDLEGPSTSLP-SSRYLNDFEELQSLGHGGFAHVVL 433

Query: 1418 CKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYG 1597
            CKNKLDGRQYAVKKIRLKDKS PLDDRILREVATLSRLQHQHVVRYYQAWFETG  G +G
Sbjct: 434  CKNKLDGRQYAVKKIRLKDKSLPLDDRILREVATLSRLQHQHVVRYYQAWFETGGVGAHG 493

Query: 1598 DATWGSRAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMFESY-QFEKK 1771
            D TWGSR AASS+FS+   SS D +G +N +  TYLYIQMEYCPRTLRQ+FESY QF+K+
Sbjct: 494  DTTWGSRTAASSTFSFKGTSSADDLGNENKLESTYLYIQMEYCPRTLRQVFESYSQFDKE 553

Query: 1772 PAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPD 1951
             AWHLFRQIVEGLAHIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQL+ DP 
Sbjct: 554  SAWHLFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDPS 613

Query: 1952 -PTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERH 2128
             P +TTG+S+DGTGQ+GTYFYTAPEIEQ WPKI+EKADMYSLGI+F ELWHPF T MERH
Sbjct: 614  FPADTTGVSLDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMERH 673

Query: 2129 VVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDN 2308
             VLSDLK+KGELPS W AEFPEQA                ATEL+KHAFPPRMESELLDN
Sbjct: 674  HVLSDLKQKGELPSAWVAEFPEQASLLRSLMSPSPSDRPSATELIKHAFPPRMESELLDN 733

Query: 2309 ILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRD 2488
            ILRTM  SED S+YDKV+ A+F EE+++ K+Q +     +L   + S+IQ+ DLD+E RD
Sbjct: 734  ILRTMQTSEDRSVYDKVLNAIFDEEMLSLKDQQHHDGRLRLAGSDTSAIQYADLDSEARD 793

Query: 2489 LVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQ-LNRNAVKLLTDGGDMIELCHELRLPFIN 2665
             V E++  VFR HCAK LE+IPM LL D    + RN VKLLT GGDM+EL HELRLPF++
Sbjct: 794  YVVEITREVFRQHCAKHLEVIPMRLLDDCQHFMRRNTVKLLTHGGDMLELLHELRLPFVS 853

Query: 2666 WVVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITR 2845
            WV+ NQKSSFKR+EI  VYRRPIGH+PP+RYLQGDFD+IGGASALTEAEVIK   DI+TR
Sbjct: 854  WVISNQKSSFKRYEISCVYRRPIGHAPPSRYLQGDFDIIGGASALTEAEVIKVTRDIVTR 913

Query: 2846 FFLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRR 3025
            F   E CDIHLNHGDLLEAIWSW+G+KADHRQKVAELLS++GSLRPQS+ERKS WVVIRR
Sbjct: 914  FVNWEFCDIHLNHGDLLEAIWSWVGVKADHRQKVAELLSMMGSLRPQSSERKSKWVVIRR 973

Query: 3026 QLRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYL 3205
            QL QELN+ E VVN+LQ VGLRFCG AD +L RLRGAL    D    KAL+ELS +  YL
Sbjct: 974  QLLQELNLQEAVVNRLQTVGLRFCGAADQSLHRLRGALPN--DKPTRKALDELSDLCSYL 1031

Query: 3206 RIWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMW 3385
            R W+I+++V+IDPLIPPTE YHRD FFQ++L K ++ G+ TEG LLA+GGRYDYLLH MW
Sbjct: 1032 RAWRIEEHVYIDPLIPPTESYHRDLFFQVYLVKDSNPGSVTEGALLAIGGRYDYLLHHMW 1091

Query: 3386 QSEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVA 3565
              EHKSNPP +VGTSLALETIIQHS VD  +P RN+ S SVLVCS+GGGGLL +RMELV 
Sbjct: 1092 GLEHKSNPPGSVGTSLALETIIQHSPVDF-KPIRNEVSNSVLVCSKGGGGLLAERMELVN 1150

Query: 3566 ELWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKE 3727
            ELWE++IKAEF+P  DPS  EQY+YA EHDIKCL+ +++      GSVK+RHLELK+EKE
Sbjct: 1151 ELWEENIKAEFLPTPDPSLTEQYEYANEHDIKCLVIITDTGVSQKGSVKVRHLELKKEKE 1210

Query: 3728 VSRESLVKFLSEAMASQFRNPSIW 3799
            V RE LV+FL +AMA QF+NPSIW
Sbjct: 1211 VEREILVRFLLDAMAIQFKNPSIW 1234


>ref|XP_021813367.1| eIF-2-alpha kinase GCN2 isoform X1 [Prunus avium]
          Length = 1243

 Score = 1482 bits (3837), Expect = 0.0
 Identities = 773/1219 (63%), Positives = 924/1219 (75%), Gaps = 37/1219 (3%)
 Frame = +2

Query: 257  DNELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVSALLSIRFLDGY 430
            DNELLSEEITALCAIFQ+DC V+S +  +I IKLRP+S+D GYEDLDVSALL +R L GY
Sbjct: 33   DNELLSEEITALCAIFQDDCKVMSGSHPQIIIKLRPHSKDMGYEDLDVSALLLVRCLPGY 92

Query: 431  PYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQEFLSEVAPLE 610
            PYKCPKLQI  EKGLS+SDAD LLSL++DQAN NAREGRVMI+NLVE AQEFLSEV P+ 
Sbjct: 93   PYKCPKLQITPEKGLSQSDADRLLSLIHDQANSNAREGRVMIFNLVETAQEFLSEVVPVS 152

Query: 611  QPH-------------LPRKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHWGLSVEEXXX 751
            Q               L +K +A+SS++  KGPFVYGF+DLF GS ESW+WGL V+E   
Sbjct: 153  QSRGSVICPTMGSSAQLFQKDIAISSNK--KGPFVYGFIDLFSGSGESWNWGLGVDETSG 210

Query: 752  XXXXL-----DDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXXXXXXXXXXXX 916
                +     D      +    K++++ + + ++D K + +L+ST K             
Sbjct: 211  INPSVPSHTVDVSKVKHEIQEKKLDRHAEPLNLQDIKKSSLLSSTVKLDSLEEDSEDGNK 270

Query: 917  XXXXXXXXXXXXX--VG-----EDYPLAXXXXXXXXXXXXXXXHSGSASAASIDHHQISH 1075
                           VG     E   L                 S S S AS+ H Q+S 
Sbjct: 271  SIASTDSSRFLLEELVGNGGKAEKENLVLEEDSTEDDCEFGSEQSESLSFASLGHDQVSQ 330

Query: 1076 DVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLFDKSFNQ 1255
             V+KDLI+  LLRLAC+SKGPLADAL  + +EL +LGI+S+   DLA++  SL +++FN 
Sbjct: 331  TVKKDLIMVHLLRLACTSKGPLADALPQITTELENLGILSEWGRDLASKPPSLLNRTFNH 390

Query: 1256 AFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVVLCKNKLD 1435
            AF    V+SR+SQFW+  SD +G  TS   SSRYL+DFEELQ LGHGGFG+VVLCKNKLD
Sbjct: 391  AFREHMVSSRVSQFWEPTSDFEGPSTSLP-SSRYLSDFEELQSLGHGGFGHVVLCKNKLD 449

Query: 1436 GRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYGDATWGS 1615
            GRQYAVKKIRLKDKS P++DRILREVATLSRLQHQHVVRYYQAWFETG  G +GD TWGS
Sbjct: 450  GRQYAVKKIRLKDKSLPVNDRILREVATLSRLQHQHVVRYYQAWFETGIVGAHGDTTWGS 509

Query: 1616 RAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMFESY-QFEKKPAWHLF 1789
              AASS+FS+   +S D +G +N +  TYLYIQMEYCPRTLRQ+FESY +F+K+ AWHL 
Sbjct: 510  MTAASSTFSFKGTNSADALGHENKLESTYLYIQMEYCPRTLRQVFESYSRFDKELAWHLC 569

Query: 1790 RQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPD-PTETT 1966
            RQIVEGLAHIH QGIIHRDLTP+NIFFDARNDIKIGDFGLAKFLKLEQL+ +P  P +T 
Sbjct: 570  RQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQEPSFPPDTA 629

Query: 1967 GMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHVVLSDL 2146
            G+S+DGTGQ+GTYFYTAPEIEQ WPKI+EKADMYSLG++F ELWHPF T MERH VLSDL
Sbjct: 630  GVSLDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGVVFFELWHPFGTAMERHHVLSDL 689

Query: 2147 KKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNILRTMH 2326
            K+KGELP  W AEFPEQA                ATELLKHAFPPRMESELLDNILRTM 
Sbjct: 690  KQKGELPPAWVAEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRTMQ 749

Query: 2327 NSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKEN-SSIQHTDLDTEIRDLVWEV 2503
             SED S+YDKV+ A+F EE+++ K+Q +     +LG+  + S+IQ+ DL TE RD V ++
Sbjct: 750  TSEDRSVYDKVLNAIFDEEMLSMKDQQHHDG--RLGSVSDISAIQYADLHTEARDYVVDI 807

Query: 2504 SSTVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWVVLNQ 2683
            +  VFR HCAK LE+I M LL D  Q NRN VKLLT GGDM+ELCHELRLPF++W++ +Q
Sbjct: 808  TREVFRQHCAKHLEVITMRLLDDCQQFNRNTVKLLTHGGDMLELCHELRLPFVSWMISSQ 867

Query: 2684 KSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFFLPES 2863
            KSSFKR+E+ YV+RRPIGHSPP+RYLQGDFD+IGGASALTEAEVIK   DI+ RFF  + 
Sbjct: 868  KSSFKRYEVSYVHRRPIGHSPPSRYLQGDFDIIGGASALTEAEVIKVTTDIVARFFNSDF 927

Query: 2864 CDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQLRQEL 3043
            CDIHLNHGDLLEAIWSW+G+K++HR KVAELLS++GSLRPQS+ERKS WVVIRRQL QEL
Sbjct: 928  CDIHLNHGDLLEAIWSWVGVKSEHRHKVAELLSMMGSLRPQSSERKSKWVVIRRQLLQEL 987

Query: 3044 NITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLRIWKID 3223
            N+ E VVN+LQ VGLRFCG AD AL RLRGAL    D    KAL+ELS +  +LR+W+I+
Sbjct: 988  NLPEAVVNRLQTVGLRFCGAADQALARLRGALP--TDKPTRKALDELSDLYSHLRVWRIE 1045

Query: 3224 KNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQSEHKS 3403
            ++V+I+PL+PPTEGYHRD FFQ++L K N+ G+ TEGTLLAVGGRYDYLL QMW  EHKS
Sbjct: 1046 RHVYINPLMPPTEGYHRDLFFQVYLVKDNNPGSLTEGTLLAVGGRYDYLLRQMWGLEHKS 1105

Query: 3404 NPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAELWEDD 3583
            +PP AVG SLALETIIQHS VD+ +P R + S  VLVCS+GGGGLL +RMELVAELWE++
Sbjct: 1106 SPPGAVGASLALETIIQHSPVDV-KPIRYEVSNDVLVCSKGGGGLLAERMELVAELWEEN 1164

Query: 3584 IKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKEVSRESL 3745
            IKAEFVPI DPS  EQY+YA EHDIKCL+ +++      GSVK+RHLELK+EKEV RE+L
Sbjct: 1165 IKAEFVPIPDPSLTEQYEYANEHDIKCLVIITDTGVSQKGSVKVRHLELKKEKEVERENL 1224

Query: 3746 VKFLSEAMASQFRNPSIWN 3802
            V+FL +AMA QF+NPSIWN
Sbjct: 1225 VRFLLDAMAIQFKNPSIWN 1243


>ref|XP_016648036.1| PREDICTED: eIF-2-alpha kinase GCN2 [Prunus mume]
          Length = 1243

 Score = 1482 bits (3836), Expect = 0.0
 Identities = 772/1219 (63%), Positives = 927/1219 (76%), Gaps = 37/1219 (3%)
 Frame = +2

Query: 257  DNELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVSALLSIRFLDGY 430
            DNELLSEEITALCAIFQ+DC V+S +  +I IKL+P+S+D GYEDLDVSALL +R L GY
Sbjct: 33   DNELLSEEITALCAIFQDDCKVMSGSHPQIIIKLKPHSKDMGYEDLDVSALLLVRCLPGY 92

Query: 431  PYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQEFLSEVAPLE 610
            PYKCPKLQI  EKGLS+SDAD LLSL++DQAN NAREGRVMI+NLVE AQEFLSEV P+ 
Sbjct: 93   PYKCPKLQITPEKGLSQSDADRLLSLIHDQANSNAREGRVMIFNLVETAQEFLSEVVPVS 152

Query: 611  QPH-------------LPRKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHWGLSVEEXXX 751
            Q H             L +K +A+SS++  KGPFVYGF+DLF GS ESW+WG  V+E   
Sbjct: 153  QSHGSVICPTMGSSAQLFQKDIAISSNK--KGPFVYGFIDLFSGSGESWNWGFGVDETSG 210

Query: 752  XXXXLDD-LPNGSQPSRD----KVNQNMKMMVVEDNKLNKVLTSTGKXXXXXXXXXXXXX 916
                +     +GS+   +    K++++ + + ++D K + +L+ST K             
Sbjct: 211  INPSVPSHTVDGSKVKHEIQEKKLDRHAEPLNLQDIKKSSLLSSTVKLDSLEEDSEDGNK 270

Query: 917  XXXXXXXXXXXXX--VG-----EDYPLAXXXXXXXXXXXXXXXHSGSASAASIDHHQISH 1075
                           VG     E   L                 S S S AS+ H Q+S 
Sbjct: 271  SIASTNSSRFLLEGLVGNGGKAEKENLVLEEDSTEDDCEFGSEQSESLSFASLGHDQVSQ 330

Query: 1076 DVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLFDKSFNQ 1255
             V+KDLI+  LLRLAC+SKGPLADAL  + +EL +LGI+S+   DLA++  SL +++FN 
Sbjct: 331  TVKKDLIMVHLLRLACTSKGPLADALPQITTELENLGILSEWGRDLASKPPSLLNRTFNH 390

Query: 1256 AFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVVLCKNKLD 1435
            AF    V+SR+SQFW+  SD +G  TS   SSRYL+DFEELQ LGHGGFG+VVLCKNKLD
Sbjct: 391  AFREHMVSSRVSQFWEPTSDFEGPSTSLP-SSRYLSDFEELQSLGHGGFGHVVLCKNKLD 449

Query: 1436 GRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYGDATWGS 1615
            GRQYAVKKIRLKDKS P++DRILREVATLSRLQHQHVVRYYQAWFETG  G +GD TWGS
Sbjct: 450  GRQYAVKKIRLKDKSLPVNDRILREVATLSRLQHQHVVRYYQAWFETGIVGAHGDTTWGS 509

Query: 1616 RAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMFESY-QFEKKPAWHLF 1789
              AASS+FS+   +S D +G +N +  TYLYIQMEYCPRTLRQ+FESY +F+K+ AWHL 
Sbjct: 510  MTAASSTFSFKGTNSADALGHENKLESTYLYIQMEYCPRTLRQVFESYSRFDKELAWHLC 569

Query: 1790 RQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPD-PTETT 1966
            RQIVEGLAHIH QGIIHRDLTP+NIFFDARNDIKIGDFGLAKFLKLEQL+ +P  P +T 
Sbjct: 570  RQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQEPSFPPDTA 629

Query: 1967 GMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHVVLSDL 2146
            G+S+DGTG++GTYFYTAPEIEQ WPKI+EKADMYSLG++F ELWHPF T MERH VL+DL
Sbjct: 630  GVSLDGTGKVGTYFYTAPEIEQGWPKIDEKADMYSLGVVFFELWHPFRTAMERHHVLTDL 689

Query: 2147 KKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNILRTMH 2326
            K+KGELP  W AEFPEQA                ATELLKHAFPPRMESELLDNILRTM 
Sbjct: 690  KQKGELPPAWVAEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRTMQ 749

Query: 2327 NSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKEN-SSIQHTDLDTEIRDLVWEV 2503
             SED S+YDKV+ A+F EE+++ K+Q +     +LG+  + S+IQ+ DL TE RD V ++
Sbjct: 750  TSEDRSVYDKVLNAIFDEEMLSMKDQQHHDG--RLGSVSDISAIQYADLHTEARDYVVDI 807

Query: 2504 SSTVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWVVLNQ 2683
            +  VFR HCAK LE+I M LL D  Q NRN VKLLT GGDM+ELCHELRLPF++WVV +Q
Sbjct: 808  TREVFRQHCAKHLEVITMRLLDDCQQFNRNTVKLLTHGGDMLELCHELRLPFVSWVVSSQ 867

Query: 2684 KSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFFLPES 2863
            KSSFKR+E+ YV+RRP+GHSPP+RYLQGDFD+IGGASALTEAEVIK   DI+  FF  + 
Sbjct: 868  KSSFKRYEVSYVHRRPVGHSPPSRYLQGDFDIIGGASALTEAEVIKVTRDIVAPFFNSDF 927

Query: 2864 CDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQLRQEL 3043
            CDIHLNHGDLLEAIWSW+G+K++HRQKVAELLS++GSLRPQS+ERKS WVVIRRQL QEL
Sbjct: 928  CDIHLNHGDLLEAIWSWVGVKSEHRQKVAELLSMMGSLRPQSSERKSKWVVIRRQLLQEL 987

Query: 3044 NITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLRIWKID 3223
            N+ E VVN+LQ VGLRFCG AD AL RLRGAL    D    KAL+ELS +  +LR+W+I+
Sbjct: 988  NLPEAVVNRLQTVGLRFCGAADQALARLRGALP--TDKPTRKALDELSDLYSHLRVWRIE 1045

Query: 3224 KNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQSEHKS 3403
            ++V+I+PL+PPTEGYHRD FFQ++L K N+ G+ TEGTLLAVGGRYDYLL QMW  EHKS
Sbjct: 1046 RHVYINPLMPPTEGYHRDLFFQVYLVKDNNPGSLTEGTLLAVGGRYDYLLRQMWGLEHKS 1105

Query: 3404 NPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAELWEDD 3583
            +PP AVG SLALETIIQHS VD+ +P R + S  VLVCS+GGGGLL +RMELVAELWE++
Sbjct: 1106 SPPGAVGASLALETIIQHSPVDV-KPIRYEVSNDVLVCSKGGGGLLAERMELVAELWEEN 1164

Query: 3584 IKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKEVSRESL 3745
            IKAEFVPI DPS  EQY+YA EHDIKCL+ +++      GSVK+RHLELK+EKEV RE+L
Sbjct: 1165 IKAEFVPIPDPSLTEQYEYANEHDIKCLVIITDTGVSQKGSVKVRHLELKKEKEVERENL 1224

Query: 3746 VKFLSEAMASQFRNPSIWN 3802
            V+FL +AMA QF+NPSIWN
Sbjct: 1225 VRFLLDAMAIQFKNPSIWN 1243


>ref|XP_024195138.1| eIF-2-alpha kinase GCN2 isoform X1 [Rosa chinensis]
 gb|PRQ37806.1| putative protein kinase PEK-GCN2 family [Rosa chinensis]
          Length = 1236

 Score = 1480 bits (3831), Expect = 0.0
 Identities = 777/1224 (63%), Positives = 911/1224 (74%), Gaps = 35/1224 (2%)
 Frame = +2

Query: 233  PATDHT-SLDNELLSEEITALCAIFQEDCDVIS--ENRIKIKLRPYSQDSGYEDLDVSAL 403
            P  DH  + DN+LLSEEITALCAIFQ+DC + S  + +I IKLRP+S+D GYEDLDVSAL
Sbjct: 22   PLKDHAGAYDNDLLSEEITALCAIFQDDCKIASGAQPQITIKLRPHSKDMGYEDLDVSAL 81

Query: 404  LSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQE 583
            L +R L GYPYKCPKLQI  EKGLSKSD+D LLSL+ DQAN NAREGRVMI+NLVEAAQE
Sbjct: 82   LLVRCLPGYPYKCPKLQITPEKGLSKSDSDKLLSLINDQANSNAREGRVMIFNLVEAAQE 141

Query: 584  FLSEVAPLEQPHLP-------------RKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHW 724
            FLSE+ P+ Q   P             +   A+SS++  KGPFVYGF+DLF GS +SW W
Sbjct: 142  FLSEIVPVGQSQGPVICSTMDNSAQLFQNDTAISSNK--KGPFVYGFIDLFSGSGKSWSW 199

Query: 725  GLSVEEXXXXXXXLDDLP--NGS-------QPSRDKVNQNMKMMVVEDNKLNKVLTSTGK 877
               V+E       +  LP  +GS       +   DK  + +K    +D K   +L+   K
Sbjct: 200  SFGVDETKGINSTVP-LPRVDGSTLMHEIQEKKHDKPAEALKS---QDIKQGPILSPNVK 255

Query: 878  XXXXXXXXXXXXXXXXXXXXXXXXXXVGEDYPLAXXXXXXXXXXXXXXXHSGSASAASID 1057
                                        E                     S S S  S+ 
Sbjct: 256  LETLEEESEGRDSSRFLLEEMDRNGGETEKESSVPEEDSTEDDWEFGSQQSDSLSFTSLA 315

Query: 1058 HHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLF 1237
            H Q S +V++DLI+  LLRLAC+ KGPLADAL  + +EL ++G++S+   DLA++  SL 
Sbjct: 316  HEQGSQNVKRDLIMVHLLRLACT-KGPLADALPQITTELQNIGVLSEWARDLASKPISLL 374

Query: 1238 DKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVVL 1417
            +++FN AFG + V+SRISQFW+  SD +G  TS   SSRYLNDFEELQ LGHGGF +VVL
Sbjct: 375  NRTFNHAFGQQMVSSRISQFWELTSDLEGPSTSLP-SSRYLNDFEELQSLGHGGFAHVVL 433

Query: 1418 CKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYG 1597
            CKNKLDGRQYAVKKIRLKDKS PLDDRILREVATLSRLQHQHVVRYYQAWFETG  G +G
Sbjct: 434  CKNKLDGRQYAVKKIRLKDKSLPLDDRILREVATLSRLQHQHVVRYYQAWFETGGVGAHG 493

Query: 1598 DATWGSRAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMFESY-QFEKK 1771
            D TWGSR AASS+FS+   SS D +G +N +  TYLYIQMEYCPRTLRQ+FESY QF+K+
Sbjct: 494  DTTWGSRTAASSTFSFKGTSSADDLGNENKLESTYLYIQMEYCPRTLRQVFESYSQFDKE 553

Query: 1772 PAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPD 1951
             AWHLFRQIVEGLAHIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQL+ DP 
Sbjct: 554  SAWHLFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDPS 613

Query: 1952 -PTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERH 2128
             P +TTG+S+DGTGQ+GTYFYTAPEIEQ WPKI+EKADMYSLGI+F ELWHPF T MERH
Sbjct: 614  FPADTTGVSLDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMERH 673

Query: 2129 VVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDN 2308
             VLSDLK+KGELPS W AEFPEQA                ATEL+KHAFPPRMESELLDN
Sbjct: 674  HVLSDLKQKGELPSAWVAEFPEQASLLRSLMSPSPSDRPSATELIKHAFPPRMESELLDN 733

Query: 2309 ILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRD 2488
            ILRTM  SED S+YDKV+ A+F EE+++ K+Q +     +L   + S+IQ+ DLD+E RD
Sbjct: 734  ILRTMQTSEDRSVYDKVLNAIFDEEMLSLKDQQHHDGRLRLAGSDTSAIQYADLDSEARD 793

Query: 2489 LVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQ-LNRNAVKLLTDGGDMIELCHELRLPFIN 2665
             V E++  VFR HCAK LE+IPM LL D    + RN VKLLT GGDM+EL HELRLPF++
Sbjct: 794  YVVEITREVFRQHCAKHLEVIPMRLLDDCQHFMRRNTVKLLTHGGDMLELLHELRLPFVS 853

Query: 2666 WVVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITR 2845
            WV+ NQKSSFKR+EI  VYRRPIGH+PP+RYLQGDFD+IGGASALTEAEVIK   DI+TR
Sbjct: 854  WVISNQKSSFKRYEISCVYRRPIGHAPPSRYLQGDFDIIGGASALTEAEVIKVTRDIVTR 913

Query: 2846 FFLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRR 3025
            F   E CDIHLNHGDLLEAIWSW+G+KADHRQKVAELLS++GSLRPQS+ERKS WVVIRR
Sbjct: 914  FVNWEFCDIHLNHGDLLEAIWSWVGVKADHRQKVAELLSMMGSLRPQSSERKSKWVVIRR 973

Query: 3026 QLRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYL 3205
            QL QELN+ E VVN+LQ VGLRFCG AD +L RLRGAL    D    KAL+ELS +  YL
Sbjct: 974  QLLQELNLQEAVVNRLQTVGLRFCGAADQSLHRLRGALPN--DKPTRKALDELSDLCSYL 1031

Query: 3206 RIWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMW 3385
            R W+I+++V+IDPLIPPTE YHRD FFQ++L K ++ G+ TEG LLA+GGRYDYLLH MW
Sbjct: 1032 RAWRIEEHVYIDPLIPPTESYHRDLFFQVYLVKDSNPGSVTEGALLAIGGRYDYLLHHMW 1091

Query: 3386 QSEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVA 3565
              EHKSNPP +VGTSLALETIIQHS VD     RN+ S SVLVCS+GGGGLL +RMELV 
Sbjct: 1092 GLEHKSNPPGSVGTSLALETIIQHSPVDFKPISRNEVSNSVLVCSKGGGGLLAERMELVN 1151

Query: 3566 ELWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKE 3727
            ELWE++IKAEF+P  DPS  EQY+YA EHDIKCL+ +++      GSVK+RHLELK+EKE
Sbjct: 1152 ELWEENIKAEFLPTPDPSLTEQYEYANEHDIKCLVIITDTGVSQKGSVKVRHLELKKEKE 1211

Query: 3728 VSRESLVKFLSEAMASQFRNPSIW 3799
            V RE LV+FL +AMA QF+NPSIW
Sbjct: 1212 VEREILVRFLLDAMAIQFKNPSIW 1235


>ref|XP_021627653.1| eIF-2-alpha kinase GCN2 isoform X1 [Manihot esculenta]
 ref|XP_021627654.1| eIF-2-alpha kinase GCN2 isoform X1 [Manihot esculenta]
 gb|OAY37680.1| hypothetical protein MANES_11G120800 [Manihot esculenta]
          Length = 1246

 Score = 1472 bits (3810), Expect = 0.0
 Identities = 762/1217 (62%), Positives = 919/1217 (75%), Gaps = 36/1217 (2%)
 Frame = +2

Query: 257  DNELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVSALLSIRFLDGY 430
            DNELL+EEITALCAIFQEDC ++SE+  +I IKLRPYS+D GYEDLDVSALLS+R + GY
Sbjct: 34   DNELLAEEITALCAIFQEDCKIVSESPPQILIKLRPYSKDMGYEDLDVSALLSVRCIPGY 93

Query: 431  PYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQEFLSEVAPL- 607
            PYKCPKLQI  EKGL+KSD DNLLSLL+DQAN NAREGRVMI+NLVEAAQEFLSE+ P+ 
Sbjct: 94   PYKCPKLQITPEKGLTKSDVDNLLSLLHDQANSNAREGRVMIFNLVEAAQEFLSEIVPVG 153

Query: 608  ---EQPHLPR--------KTVAVSSDR--PSKGPFVYGFLDLFIGSEESWHWGLSVEEXX 748
               E   L          K +AVSS++   S  PFV GF+DLF GS ESW WGL+V++  
Sbjct: 154  PVPESALLSTMDGSDQLFKDIAVSSNKNCSSCEPFVSGFIDLFSGSGESWDWGLAVDDTR 213

Query: 749  XXXXX-----LDDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXXXXXXXXXXX 913
                      LD    G +    K+++  + + V+D K + +L    K            
Sbjct: 214  AMNSSIKSHSLDGSKVGYEVLEKKLDKATRPLTVQDAKQSPLLFPVAKLGTLEEESEDDN 273

Query: 914  XXXXXXXXXXXXXX-VGEDYPLAXXXXXXXXXXXXXXXHSGSA-----SAASIDHHQISH 1075
                           VG +                   +S S      S+AS+DH Q + 
Sbjct: 274  RSISTDSSTSVTEESVGNELESKKEDDFFEEKGPEDDGNSESEPWDLLSSASLDHDQATQ 333

Query: 1076 DVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLFDKSFNQ 1255
             +EKDL++  +LRLAC+SKG LADAL+ + +EL +LG++S+   DLA++ SS+F+K+F+ 
Sbjct: 334  TIEKDLVMVHMLRLACASKGGLADALSQITTELCNLGVLSEWARDLASKPSSVFNKTFDH 393

Query: 1256 AFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVVLCKNKLD 1435
             F    V+S+ISQFWK  SD  G + S + +SRYL+DFEELQPLGHGGFG+VVLCKNKLD
Sbjct: 394  IFHQHAVSSKISQFWKPTSDLGGTNMSLS-NSRYLSDFEELQPLGHGGFGHVVLCKNKLD 452

Query: 1436 GRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYGDATWGS 1615
            GRQYAVKKIRLKDKS P++DRILREVATLSRLQHQHVVRYYQAWFETG AG +GD TWGS
Sbjct: 453  GRQYAVKKIRLKDKSLPVNDRILREVATLSRLQHQHVVRYYQAWFETGVAGSFGDTTWGS 512

Query: 1616 RAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMFESYQ-FEKKPAWHLF 1789
              A SS+FSY   +S DV GQDN +  TYLYIQMEYCPRTLRQ+FESY  F+++ AWHLF
Sbjct: 513  TTATSSTFSYRGANSADV-GQDNKLESTYLYIQMEYCPRTLRQVFESYNHFDQELAWHLF 571

Query: 1790 RQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPD-PTETT 1966
            RQIVEGLAHIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQL+ D   PT+TT
Sbjct: 572  RQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLDHDTTLPTDTT 631

Query: 1967 GMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHVVLSDL 2146
            G+S+DGTGQ+GTYFYTAPEIEQ WPKI+EKADMYSLG++F ELWHPF T MERH++LSDL
Sbjct: 632  GVSLDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGVVFFELWHPFGTAMERHIILSDL 691

Query: 2147 KKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNILRTMH 2326
            K+KG LP  W ++FPEQ                 AT+LLKHAFPPRMESELLDNILRTM 
Sbjct: 692  KQKGVLPPSWVSQFPEQTSLLQRLMSPSPSDRPSATDLLKHAFPPRMESELLDNILRTMK 751

Query: 2327 NSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRDLVWEVS 2506
             SED ++YDKVV ++F EE+++ K+Q   S    +G  + SS+Q+ DLDTE+RD + E +
Sbjct: 752  TSEDRTVYDKVVSSIFDEEMLSMKSQHQLSGRLGMGGGDTSSVQYADLDTELRDYIVEAT 811

Query: 2507 STVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWVVLNQK 2686
              VF+ H AK LEI+ M LL D  Q +R+ VKLLT GGD++ELCHELRLPF++W+V NQK
Sbjct: 812  REVFKQHSAKHLEIVSMRLLDDCPQFSRSTVKLLTHGGDLLELCHELRLPFVSWLVANQK 871

Query: 2687 SSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFFLPESC 2866
            SSFKR+EI  VYRR IGHSPPNRYLQGDFD++GG SAL EAEVIK +MDI+T+FF+P SC
Sbjct: 872  SSFKRYEISPVYRRAIGHSPPNRYLQGDFDIVGGVSALMEAEVIKVSMDIVTQFFVPGSC 931

Query: 2867 DIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQLRQELN 3046
            DIHLNHGD+L+AIWSWIGIK +HRQKVAELLSL+GSLRPQS+ERK  W VIRRQL QELN
Sbjct: 932  DIHLNHGDILDAIWSWIGIKPEHRQKVAELLSLMGSLRPQSSERKLKWGVIRRQLLQELN 991

Query: 3047 ITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLRIWKIDK 3226
            + E VVN+LQ VGLRFCG AD A+PRLRGAL    D    KAL+ELS +++YLR+WKI+ 
Sbjct: 992  LAEAVVNRLQTVGLRFCGAADQAVPRLRGALP--ADKPTRKALDELSDLIVYLRVWKIED 1049

Query: 3227 NVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQSEHKSN 3406
            +V+I+ L+PPTE YHRD FFQI+L K N+ G+ +EGTLLAVGGRYDYLL QMW  ++++N
Sbjct: 1050 HVYINALMPPTENYHRDLFFQIYLMKDNNPGSLSEGTLLAVGGRYDYLLRQMWDHKYRTN 1109

Query: 3407 PPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAELWEDDI 3586
            PP AVGTSLALETIIQHS VD  RP RN++  ++LVCSRGGGGLL +RMELVAELWE +I
Sbjct: 1110 PPGAVGTSLALETIIQHSPVDF-RPLRNEACTNILVCSRGGGGLLVERMELVAELWEANI 1168

Query: 3587 KAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKEVSRESLV 3748
            KAEFVP+ DPS  EQY+YA EHDI+CL+ +++      G VK+RHLELK+EKEV RE LV
Sbjct: 1169 KAEFVPVPDPSLTEQYEYASEHDIRCLVLITDAGLSQKGFVKVRHLELKKEKEVEREKLV 1228

Query: 3749 KFLSEAMASQFRNPSIW 3799
             FL  AMA+QFRNPS+W
Sbjct: 1229 SFLLNAMATQFRNPSVW 1245


>ref|XP_015901852.1| PREDICTED: eIF-2-alpha kinase GCN2 isoform X2 [Ziziphus jujuba]
          Length = 1243

 Score = 1470 bits (3806), Expect = 0.0
 Identities = 765/1217 (62%), Positives = 904/1217 (74%), Gaps = 28/1217 (2%)
 Frame = +2

Query: 233  PATDHTSLD----NELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDV 394
            P  DH + D    NELLSEEITALCAIFQEDC V+S +  +I IK+RPYS+D G+EDLDV
Sbjct: 22   PLKDHAAHDGVDDNELLSEEITALCAIFQEDCKVVSGSAPQIMIKIRPYSKDMGFEDLDV 81

Query: 395  SALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEA 574
            +ALL +R L GYPYKCPKLQI  EKGLS++DAD LLSLL+DQAN NAREGRVMI+NLVEA
Sbjct: 82   TALLVVRCLPGYPYKCPKLQITPEKGLSENDADKLLSLLHDQANSNAREGRVMIFNLVEA 141

Query: 575  AQEFLSEVAPLEQP------HLPRKTVAVSSDRPSKGPFVYGFLDLFIGSEESWHWGLSV 736
            AQEFLSE+ P+          L  + VA+S+    +GPFVYGF+DLF GS ESW+WG  +
Sbjct: 142  AQEFLSEIVPVSCSSTDSISQLFLQDVAISNS--IRGPFVYGFIDLFSGSGESWNWGFGI 199

Query: 737  E-----EXXXXXXXLDDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXXXXXXX 901
            +             +DD    ++     + ++++  +++D K   +L+ T K        
Sbjct: 200  DGNSGTNTLVPPHTVDDSKLRNEVKEKNLEKHVRPTLLQDTKQASLLSPTAKLHPLEEES 259

Query: 902  XXXXXXXXXXXXXXXXXXV---GEDYPLAXXXXXXXXXXXXXXXHSGSASAASIDHHQIS 1072
                                    D                    S S S   +  +Q+S
Sbjct: 260  EESNKSLSSSDASESLQEKLLGNGDKGEKGFPIEEETTDDAEFEGSESLSFTYLADNQVS 319

Query: 1073 HDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLFDKSFN 1252
               EKDLI+  LLRLAC+SKGPL DAL  V +EL +LGI+S+   DLA++ S LF+K+F+
Sbjct: 320  KTTEKDLIMVHLLRLACTSKGPLTDALQQVTTELYNLGILSEWARDLASKPSPLFNKTFD 379

Query: 1253 QAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVVLCKNKL 1432
             AF    V+S+ISQFW  AS+  G +TS   SSRYLNDFEELQ LGHGGFG+VVLCKNKL
Sbjct: 380  HAFKQHMVSSKISQFWTPASE--GANTSLP-SSRYLNDFEELQSLGHGGFGHVVLCKNKL 436

Query: 1433 DGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYGDATWG 1612
            DGRQYAVKKIRLKDKS P++DRILREVATLSRLQHQHVVRYYQAWFETG A  YGD TWG
Sbjct: 437  DGRQYAVKKIRLKDKSLPVNDRILREVATLSRLQHQHVVRYYQAWFETGIADSYGDVTWG 496

Query: 1613 SRAAASSSFSYLDQSSTDVIGQDNNVG-TYLYIQMEYCPRTLRQMFESYQFEKKPAWHLF 1789
            SR AASS+FS +  SS D  GQ+N +  TYLYIQMEYCPRTLRQ+F+SY F+K+ AWH F
Sbjct: 497  SRTAASSTFSLMGTSSADAFGQENKLDPTYLYIQMEYCPRTLRQVFDSYHFDKELAWHFF 556

Query: 1790 RQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPD-PTETT 1966
            RQIVEGL HIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQ + DP  P +TT
Sbjct: 557  RQIVEGLVHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQFDQDPSFPADTT 616

Query: 1967 GMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERHVVLSDL 2146
            G+S D TGQ+GTYFYTAPEIEQ WPKI+EKADMYSLGI+F ELWHPF T MERH+VLSDL
Sbjct: 617  GLSADRTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMERHIVLSDL 676

Query: 2147 KKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDNILRTMH 2326
            K+KGE PS W AEFPEQ                 ATELL+HAFPPRMESELLDNILRTM 
Sbjct: 677  KQKGEFPSSWIAEFPEQTSLLRRLLSASPSDRPSATELLQHAFPPRMESELLDNILRTMQ 736

Query: 2327 NSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRDLVWEVS 2506
             SED+SIY KVV A+F EEI++ K+Q + +    L   + S+IQ+ DLDTEIRD V E++
Sbjct: 737  TSEDSSIYTKVVNAIFDEEILSVKDQHHHAGGLSLAGGDTSAIQYADLDTEIRDYVVEIT 796

Query: 2507 STVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINWVVLNQK 2686
              +FR HCAK LE+IPM LL D  Q NRN VKLL  GGDM+ELCHELRLPF+NWVV  QK
Sbjct: 797  RDIFRKHCAKHLEVIPMRLLDDCPQFNRNTVKLLNHGGDMLELCHELRLPFVNWVVSTQK 856

Query: 2687 SSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRFFLPESC 2866
             SFKR+EI  VYRR IGHSPPNRYLQGDFD+IGG   LTEAEVIK  +DIIT FF  +SC
Sbjct: 857  FSFKRYEISCVYRRAIGHSPPNRYLQGDFDIIGGTVTLTEAEVIKVTLDIITHFFHSDSC 916

Query: 2867 DIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQLRQELN 3046
            DIHLNHGDLL+AIWSWIG+KA+HR KVAELLS++GSLRPQS+ERKS W VIRRQL QELN
Sbjct: 917  DIHLNHGDLLDAIWSWIGVKAEHRHKVAELLSMMGSLRPQSSERKSKWAVIRRQLLQELN 976

Query: 3047 ITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLRIWKIDK 3226
            + E +VN+LQ V LRFCG AD ALPRLRGAL    D  A KAL+ELS +  YLR+W+I++
Sbjct: 977  LPEAIVNRLQTVALRFCGAADQALPRLRGALP--ADKSARKALDELSDLFSYLRVWRIER 1034

Query: 3227 NVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQSEHKSN 3406
            +V+ID L+ PTEGYHRD FFQ++L K +S G+  EG LLAVGG YDYLLHQ+W  E+KSN
Sbjct: 1035 HVYIDALMTPTEGYHRDLFFQVYLIKEHSSGSLVEGALLAVGGCYDYLLHQLWAQEYKSN 1094

Query: 3407 PPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAELWEDDI 3586
            PP AVG+SLALETIIQ+S VD  +P RN++S S+LVCSRGGGGLL +RMELVAELWE++I
Sbjct: 1095 PPGAVGSSLALETIIQYSPVDF-KPIRNEASTSILVCSRGGGGLLVERMELVAELWENNI 1153

Query: 3587 KAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKEVSRESLV 3748
            KAEFVP  DPS  EQY+YA EHDIKCL+ +++       +VK+RHLELK+EKEV R+ +V
Sbjct: 1154 KAEFVPNPDPSLTEQYEYANEHDIKCLVIITDTGMVHKDTVKVRHLELKKEKEVERDGVV 1213

Query: 3749 KFLSEAMASQFRNPSIW 3799
            KFL EA+A+QFRNPSIW
Sbjct: 1214 KFLLEAIATQFRNPSIW 1230


>ref|XP_015901851.1| PREDICTED: eIF-2-alpha kinase GCN2 isoform X1 [Ziziphus jujuba]
          Length = 1250

 Score = 1468 bits (3800), Expect = 0.0
 Identities = 765/1224 (62%), Positives = 904/1224 (73%), Gaps = 35/1224 (2%)
 Frame = +2

Query: 233  PATDHTSLD----NELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDV 394
            P  DH + D    NELLSEEITALCAIFQEDC V+S +  +I IK+RPYS+D G+EDLDV
Sbjct: 22   PLKDHAAHDGVDDNELLSEEITALCAIFQEDCKVVSGSAPQIMIKIRPYSKDMGFEDLDV 81

Query: 395  SALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEA 574
            +ALL +R L GYPYKCPKLQI  EKGLS++DAD LLSLL+DQAN NAREGRVMI+NLVEA
Sbjct: 82   TALLVVRCLPGYPYKCPKLQITPEKGLSENDADKLLSLLHDQANSNAREGRVMIFNLVEA 141

Query: 575  AQEFLSEVAPLEQPH-------------LPRKTVAVSSDRPSKGPFVYGFLDLFIGSEES 715
            AQEFLSE+ P+                 L  + VA+S+    +GPFVYGF+DLF GS ES
Sbjct: 142  AQEFLSEIVPVGHSQTSVSCSSTDSISQLFLQDVAISNS--IRGPFVYGFIDLFSGSGES 199

Query: 716  WHWGLSVE-----EXXXXXXXLDDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKX 880
            W+WG  ++             +DD    ++     + ++++  +++D K   +L+ T K 
Sbjct: 200  WNWGFGIDGNSGTNTLVPPHTVDDSKLRNEVKEKNLEKHVRPTLLQDTKQASLLSPTAKL 259

Query: 881  XXXXXXXXXXXXXXXXXXXXXXXXXV---GEDYPLAXXXXXXXXXXXXXXXHSGSASAAS 1051
                                           D                    S S S   
Sbjct: 260  HPLEEESEESNKSLSSSDASESLQEKLLGNGDKGEKGFPIEEETTDDAEFEGSESLSFTY 319

Query: 1052 IDHHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSS 1231
            +  +Q+S   EKDLI+  LLRLAC+SKGPL DAL  V +EL +LGI+S+   DLA++ S 
Sbjct: 320  LADNQVSKTTEKDLIMVHLLRLACTSKGPLTDALQQVTTELYNLGILSEWARDLASKPSP 379

Query: 1232 LFDKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNV 1411
            LF+K+F+ AF    V+S+ISQFW  AS+  G +TS   SSRYLNDFEELQ LGHGGFG+V
Sbjct: 380  LFNKTFDHAFKQHMVSSKISQFWTPASE--GANTSLP-SSRYLNDFEELQSLGHGGFGHV 436

Query: 1412 VLCKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGC 1591
            VLCKNKLDGRQYAVKKIRLKDKS P++DRILREVATLSRLQHQHVVRYYQAWFETG A  
Sbjct: 437  VLCKNKLDGRQYAVKKIRLKDKSLPVNDRILREVATLSRLQHQHVVRYYQAWFETGIADS 496

Query: 1592 YGDATWGSRAAASSSFSYLDQSSTDVIGQDNNVG-TYLYIQMEYCPRTLRQMFESYQFEK 1768
            YGD TWGSR AASS+FS +  SS D  GQ+N +  TYLYIQMEYCPRTLRQ+F+SY F+K
Sbjct: 497  YGDVTWGSRTAASSTFSLMGTSSADAFGQENKLDPTYLYIQMEYCPRTLRQVFDSYHFDK 556

Query: 1769 KPAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDP 1948
            + AWH FRQIVEGL HIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQ + DP
Sbjct: 557  ELAWHFFRQIVEGLVHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQFDQDP 616

Query: 1949 D-PTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMER 2125
              P +TTG+S D TGQ+GTYFYTAPEIEQ WPKI+EKADMYSLGI+F ELWHPF T MER
Sbjct: 617  SFPADTTGLSADRTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMER 676

Query: 2126 HVVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLD 2305
            H+VLSDLK+KGE PS W AEFPEQ                 ATELL+HAFPPRMESELLD
Sbjct: 677  HIVLSDLKQKGEFPSSWIAEFPEQTSLLRRLLSASPSDRPSATELLQHAFPPRMESELLD 736

Query: 2306 NILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIR 2485
            NILRTM  SED+SIY KVV A+F EEI++ K+Q + +    L   + S+IQ+ DLDTEIR
Sbjct: 737  NILRTMQTSEDSSIYTKVVNAIFDEEILSVKDQHHHAGGLSLAGGDTSAIQYADLDTEIR 796

Query: 2486 DLVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFIN 2665
            D V E++  +FR HCAK LE+IPM LL D  Q NRN VKLL  GGDM+ELCHELRLPF+N
Sbjct: 797  DYVVEITRDIFRKHCAKHLEVIPMRLLDDCPQFNRNTVKLLNHGGDMLELCHELRLPFVN 856

Query: 2666 WVVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITR 2845
            WVV  QK SFKR+EI  VYRR IGHSPPNRYLQGDFD+IGG   LTEAEVIK  +DIIT 
Sbjct: 857  WVVSTQKFSFKRYEISCVYRRAIGHSPPNRYLQGDFDIIGGTVTLTEAEVIKVTLDIITH 916

Query: 2846 FFLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRR 3025
            FF  +SCDIHLNHGDLL+AIWSWIG+KA+HR KVAELLS++GSLRPQS+ERKS W VIRR
Sbjct: 917  FFHSDSCDIHLNHGDLLDAIWSWIGVKAEHRHKVAELLSMMGSLRPQSSERKSKWAVIRR 976

Query: 3026 QLRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYL 3205
            QL QELN+ E +VN+LQ V LRFCG AD ALPRLRGAL    D  A KAL+ELS +  YL
Sbjct: 977  QLLQELNLPEAIVNRLQTVALRFCGAADQALPRLRGALP--ADKSARKALDELSDLFSYL 1034

Query: 3206 RIWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMW 3385
            R+W+I+++V+ID L+ PTEGYHRD FFQ++L K +S G+  EG LLAVGG YDYLLHQ+W
Sbjct: 1035 RVWRIERHVYIDALMTPTEGYHRDLFFQVYLIKEHSSGSLVEGALLAVGGCYDYLLHQLW 1094

Query: 3386 QSEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVA 3565
              E+KSNPP AVG+SLALETIIQ+S VD  +P RN++S S+LVCSRGGGGLL +RMELVA
Sbjct: 1095 AQEYKSNPPGAVGSSLALETIIQYSPVDF-KPIRNEASTSILVCSRGGGGLLVERMELVA 1153

Query: 3566 ELWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKE 3727
            ELWE++IKAEFVP  DPS  EQY+YA EHDIKCL+ +++       +VK+RHLELK+EKE
Sbjct: 1154 ELWENNIKAEFVPNPDPSLTEQYEYANEHDIKCLVIITDTGMVHKDTVKVRHLELKKEKE 1213

Query: 3728 VSRESLVKFLSEAMASQFRNPSIW 3799
            V R+ +VKFL EA+A+QFRNPSIW
Sbjct: 1214 VERDGVVKFLLEAIATQFRNPSIW 1237


>gb|AMY99278.1| serine/threonine-protein kinase GCN2 [Nicotiana tabacum]
          Length = 1252

 Score = 1466 bits (3795), Expect = 0.0
 Identities = 763/1230 (62%), Positives = 914/1230 (74%), Gaps = 43/1230 (3%)
 Frame = +2

Query: 242  DHTSL---DNELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVSALL 406
            DH S+   ++ELL+EE+TALCAIFQEDC+V+SE+  ++ IKLRPYS+D+GYED DVSALL
Sbjct: 26   DHNSIGDGNSELLAEEVTALCAIFQEDCEVVSESPSQLHIKLRPYSKDAGYEDSDVSALL 85

Query: 407  SIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQEF 586
            S+R L GYPYK PKLQI  EKGLSK+DA+NLLSLLYDQA+ NAREGRVMIYNLVEAAQEF
Sbjct: 86   SVRCLPGYPYKTPKLQIIPEKGLSKADANNLLSLLYDQASSNAREGRVMIYNLVEAAQEF 145

Query: 587  LSEVAPLEQPH-------------LPRKTVAVSSDRPSK--GPFVYGFLDLFIGSEESWH 721
            LSE+ P E  H             L  K    SS       GPFVYGFLDLF GS ESWH
Sbjct: 146  LSEIVPQEHLHGSVSCQLTYITSQLTYKDAKASSGDTCSFGGPFVYGFLDLFSGSGESWH 205

Query: 722  WGLSVEEXXXXXXXL-----DDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXX 886
            W L + +       +     D L +     + K++Q +K  + E  K + +  +  K   
Sbjct: 206  WSLGMNDTGRIDSLVQSHVSDGLKHEYDNQQKKIDQIVKPALNEAAKQDSLRKAEMKLDD 265

Query: 887  XXXXXXXXXXXXXXXXXXXXXXXVGEDYPLAXXXXXXXXXXXXXXXH-------SGSASA 1045
                                     ED+ +                        S   S+
Sbjct: 266  LEEESVGESKSCSDLSKSYTDEST-EDHVMCKNIFLEGNISDSGDSQRETEPKPSELVSS 324

Query: 1046 ASIDHHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATES 1225
             S+    + H +EKDLILA LLRLAC  KGPL+DAL ++ SEL  LGIVS+RV DLA++ 
Sbjct: 325  GSLVQDHLPHTLEKDLILAHLLRLACGPKGPLSDALPEITSELFDLGIVSERVQDLASKP 384

Query: 1226 SSLFDKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFG 1405
            SS FD +F++ F    V+S++SQFWK +S+ +GQ++SP  +SRYLNDFEELQPLG GGFG
Sbjct: 385  SSFFDGTFDRVFQAHKVSSKLSQFWKASSEFEGQNSSPPQNSRYLNDFEELQPLGQGGFG 444

Query: 1406 NVVLCKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDA 1585
            +VVLCKNKLDGRQYAVKKIRLKDKS PL+DRILREVATLSRLQHQHVVRYYQAWFETG  
Sbjct: 445  HVVLCKNKLDGRQYAVKKIRLKDKSLPLNDRILREVATLSRLQHQHVVRYYQAWFETGIT 504

Query: 1586 GCYGDATWGSRAAASSSFSYLDQSSTDVIGQDNNVG-TYLYIQMEYCPRTLRQMFESYQ- 1759
              Y D++ GSR   SSSF+Y+D S +D +GQ+N +  TYLYIQMEYCPRTLRQMFESY  
Sbjct: 505  LSYDDSSCGSRTVVSSSFTYVDGSMSDHLGQENKLELTYLYIQMEYCPRTLRQMFESYSN 564

Query: 1760 FEKKPAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLE 1939
             +K+ AWHLFRQIVEGLAHIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQL+
Sbjct: 565  LDKELAWHLFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLD 624

Query: 1940 PDPDPTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGM 2119
             D D  E  G+S+DGTGQ+GTYFYTAPEIEQRWPKINEKADMYSLG++F ELWHPF T M
Sbjct: 625  QDVDAAEMIGVSVDGTGQVGTYFYTAPEIEQRWPKINEKADMYSLGVVFFELWHPFDTAM 684

Query: 2120 ERHVVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESEL 2299
            ERH+VLSDLK+KGE+PS WAAEFPEQA                A ELL++ FPPRME E+
Sbjct: 685  ERHIVLSDLKQKGEVPSAWAAEFPEQASLLRRLMTPSPSDRPSAVELLQNDFPPRMEYEM 744

Query: 2300 LDNILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTE 2479
            LDNILRT+H S+DT +YDK+V A+F+E+ +NTK  +   E  K+  ++ S I  TDL TE
Sbjct: 745  LDNILRTVHISDDTGVYDKIVNAIFNEDTLNTKGHNMNLESSKVVGRDTSCILFTDLQTE 804

Query: 2480 IRDLVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQLN---RNAVKLLTDGGDMIELCHELR 2650
             RD V E+S+ VFR HCAK  EIIPM +LG+  Q N   R+AVKLLT GGD++ELCHELR
Sbjct: 805  RRDNVVEISTEVFRRHCAKHFEIIPMRMLGECPQENSRERSAVKLLTHGGDVVELCHELR 864

Query: 2651 LPFINWVVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAM 2830
            LPF+ W++ N++S FKR+EI YVYRR IGHSPPNRYLQGDFD+IGG +ALTE+E+IKA M
Sbjct: 865  LPFVKWIIANRRSFFKRYEISYVYRRAIGHSPPNRYLQGDFDIIGGETALTESEIIKATM 924

Query: 2831 DIITRFFLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMW 3010
            DII  +F  ESCDIHLNH DLL+AIW+W GI+ +HRQKVAELLSLLGSLRPQS+ERK+ W
Sbjct: 925  DIILHYFHSESCDIHLNHADLLDAIWAWAGIRPEHRQKVAELLSLLGSLRPQSSERKTKW 984

Query: 3011 VVIRRQLRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQ 3190
            VVIRRQLRQELN+ E  VN+LQ VGLRFCG+ADHALPRLRGAL    D    KAL++LS+
Sbjct: 985  VVIRRQLRQELNLAETAVNRLQTVGLRFCGVADHALPRLRGALP--ADKTTRKALDDLSE 1042

Query: 3191 ILMYLRIWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYL 3370
            +  YLR+W++D+ V+ID L+PPTE YHR  FFQI+LRK + +G+  EGTLLAVGGRYDYL
Sbjct: 1043 LFNYLRVWRLDQRVYIDALMPPTESYHRKLFFQIYLRKDDHLGSLMEGTLLAVGGRYDYL 1102

Query: 3371 LHQMWQSEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKR 3550
            LHQ   SE KSNPP AVG+SLALETI+QH++    RP+R D   +VLVCSRGGGGLL +R
Sbjct: 1103 LHQSGDSECKSNPPGAVGSSLALETILQHAASLEIRPYRYDVVTNVLVCSRGGGGLLIER 1162

Query: 3551 MELVAELWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLEL 3712
            MEL+AELWE++I+AEFVP+ DPS  EQY+YA EHDIKCL+ +++       SVK+RHLEL
Sbjct: 1163 MELLAELWEENIRAEFVPLCDPSLTEQYEYANEHDIKCLVIITDTGVSQKDSVKVRHLEL 1222

Query: 3713 KREKEVSRESLVKFLSEAMASQFRNPSIWN 3802
            K+EKEV   +LVKFL EAM+SQFRNPSIWN
Sbjct: 1223 KKEKEVESGNLVKFLLEAMSSQFRNPSIWN 1252


>ref|XP_024030685.1| eIF-2-alpha kinase GCN2 isoform X2 [Morus notabilis]
          Length = 1245

 Score = 1464 bits (3790), Expect = 0.0
 Identities = 767/1233 (62%), Positives = 911/1233 (73%), Gaps = 43/1233 (3%)
 Frame = +2

Query: 233  PATDHTSL----DNELLSEEITALCAIFQEDCDVIS--ENRIKIKLRPYSQDSGYEDLDV 394
            P  DH S     DNELLSEEITALCAIFQEDC  +     ++ IKLRPYS+D G+ED+DV
Sbjct: 23   PLKDHASHESADDNELLSEEITALCAIFQEDCKFLPGPPPQVFIKLRPYSKDMGFEDVDV 82

Query: 395  SALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEA 574
            SA+L +R L GYPYKCPKLQI  EKGLS++DAD LLSLL+DQAN NAREGRVMI+NLVEA
Sbjct: 83   SAVLLVRCLPGYPYKCPKLQITPEKGLSENDADKLLSLLHDQANSNAREGRVMIFNLVEA 142

Query: 575  AQEFLSEVAPLEQPHLPRKTVAVSSDRPSK--------------GPFVYGFLDLFIGSEE 712
            AQEFLSE+ P+ Q       ++ S D  SK              GPFVYGF+DLF GS E
Sbjct: 143  AQEFLSEIVPVGQSQ--ELVLSSSMDSGSKLLYQDNAISTSNLGGPFVYGFVDLFSGSGE 200

Query: 713  SWHWGLSVEEXXXXXXXLD----DLPNGSQPSRDKVNQN-MKMMVVEDNKLNKVLTSTGK 877
            SW+WG  ++E       +     D P       +K  QN +  +V ++ + + +L+   K
Sbjct: 201  SWNWGFGLDENSVIKTSVPARTVDGPKTKNKVEEKHLQNYVGPLVFQETRKDSLLSPMTK 260

Query: 878  XXXXXXXXXXXXXXXXXXXXXXXXXX--VG-------EDYPLAXXXXXXXXXXXXXXXHS 1030
                                        VG       ED+ L                 S
Sbjct: 261  LRTLEEESEEGDKNLSSSNSSTSSVEKSVGNSSTGGEEDFSLVEHIEEDDKGSEC----S 316

Query: 1031 GSASAASIDHHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVAD 1210
             S S+AS+ H ++    +KDLI+  LLRLAC+S   L++A+  +++EL +LGI S++  +
Sbjct: 317  NSLSSASLSHERVPQTSQKDLIMVHLLRLACTSNVALSNAMQQISTELYNLGIFSEQAKE 376

Query: 1211 LATESSSLFDKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLG 1390
            LA+E+S++F+++F+ AF    V+S++S+FWK  SD +G  TS   +SRYLNDFEELQ LG
Sbjct: 377  LASEASTVFNRTFDNAFRQYMVSSKLSEFWKYTSDIEGPRTSLP-NSRYLNDFEELQSLG 435

Query: 1391 HGGFGNVVLCKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWF 1570
            HGGFG+VVLCKNK+DGRQYAVKKIRLKDKS PL+DRILREVATLSRLQHQHVVRYYQAWF
Sbjct: 436  HGGFGHVVLCKNKIDGRQYAVKKIRLKDKSLPLNDRILREVATLSRLQHQHVVRYYQAWF 495

Query: 1571 ETGDAGCYGDATWGSRAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMF 1747
            ETG +G YGDA WGSR A  S+FS+   SS D IGQDN +  TYLYIQMEYCPRTLRQ+F
Sbjct: 496  ETGISGSYGDAIWGSRTAGGSNFSFTGTSSADTIGQDNKLESTYLYIQMEYCPRTLRQVF 555

Query: 1748 ESYQ-FEKKPAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK 1924
            + Y  F+K+ AWH FRQIVEGLAHIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK
Sbjct: 556  DPYSHFDKELAWHFFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK 615

Query: 1925 LEQLEPDPD-PTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWH 2101
            LEQLE D   P ETTG+S+DGTGQ+GTYFYTAPEIEQ WPKI+EKADMYSLGI+F ELWH
Sbjct: 616  LEQLEQDLSYPAETTGVSVDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 675

Query: 2102 PFSTGMERHVVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPP 2281
            PF T MERHVVLSDLK+KGELPS W AEFPEQA                A ELL HAFPP
Sbjct: 676  PFGTAMERHVVLSDLKQKGELPSAWVAEFPEQASLLRRLMSPSPSDRPSAAELLHHAFPP 735

Query: 2282 RMESELLDNILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQH 2461
            RMESELLDNILRTM  SED++IYDKVV A+F EE+++ K+Q N +   +L     S+IQ+
Sbjct: 736  RMESELLDNILRTMQTSEDSNIYDKVVNAIFDEEVLSIKDQHNHTGRFRLPGGGTSAIQY 795

Query: 2462 TDLDTEIRDLVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCH 2641
             +LDTEIRD V E+S+ VFR HCA+ LEII M L  D  Q NRN VKLL+ GGDM+ELCH
Sbjct: 796  AELDTEIRDYVTEISNEVFRKHCARHLEIISMRLSDDCPQFNRNTVKLLSHGGDMLELCH 855

Query: 2642 ELRLPFINWVVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIK 2821
            ELRLPF++WV+ +QKSSFKRFEI  VYRR IGHSPPNRYLQGDFD+IGGASA+TEAE+IK
Sbjct: 856  ELRLPFVSWVIYSQKSSFKRFEISCVYRRAIGHSPPNRYLQGDFDIIGGASAITEAEIIK 915

Query: 2822 AAMDIITRFFLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERK 3001
              MDI+  F  P  C++HLNHG+LLE+IWSW G+KA+HR KVAELLS++ SLRPQSTERK
Sbjct: 916  VTMDILIHFLNPHCCELHLNHGNLLESIWSWTGVKAEHRHKVAELLSMMDSLRPQSTERK 975

Query: 3002 SMWVVIRRQLRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEE 3181
            S WVVIRRQL QELN+ E VVN+LQ VGLRFCG AD ALPRLRGAL    D    KAL+E
Sbjct: 976  SKWVVIRRQLLQELNLPEAVVNRLQTVGLRFCGPADQALPRLRGALPS--DKATRKALDE 1033

Query: 3182 LSQILMYLRIWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRY 3361
            LS +  YLR+W+I+++V+IDPL+PPTE YHR+ FFQ++L K N  GT  +G LLA+GGRY
Sbjct: 1034 LSDLCSYLRVWRIERHVYIDPLMPPTEIYHRELFFQVYLMKENLSGTLVDGILLAIGGRY 1093

Query: 3362 DYLLHQMWQSEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLL 3541
            DYLLHQMW  EHKS+ P  VGTSLALETIIQHS VD+ +P RND S S+LVCSRGGGGLL
Sbjct: 1094 DYLLHQMWVQEHKSHAPGGVGTSLALETIIQHSLVDL-KPIRNDDSVSILVCSRGGGGLL 1152

Query: 3542 EKRMELVAELWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRH 3703
            E+ MELVAELWE++IKAEFVP  DPS  EQY+YA EHDIKCL+ + +      GSVK+RH
Sbjct: 1153 EEWMELVAELWEENIKAEFVPKPDPSLTEQYEYASEHDIKCLVIIRDTGVAHMGSVKVRH 1212

Query: 3704 LELKREKEVSRESLVKFLSEAMASQFRNPSIWN 3802
            LE+K+EKEV RE+LVKFL +AMA QFRNPSIWN
Sbjct: 1213 LEIKKEKEVERENLVKFLLDAMAVQFRNPSIWN 1245


>ref|XP_019253353.1| PREDICTED: eIF-2-alpha kinase GCN2 isoform X1 [Nicotiana attenuata]
          Length = 1252

 Score = 1461 bits (3782), Expect = 0.0
 Identities = 761/1230 (61%), Positives = 913/1230 (74%), Gaps = 43/1230 (3%)
 Frame = +2

Query: 242  DHTSL---DNELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVSALL 406
            DH S+   ++ELL+EE+TALCAIFQEDC+V+SE+  ++ IKLRPYS+D+GYED DVSALL
Sbjct: 26   DHNSIGDGNSELLAEEVTALCAIFQEDCEVVSESPSQLHIKLRPYSKDAGYEDSDVSALL 85

Query: 407  SIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQEF 586
            S+R L GYPYK PKLQI  EKGLSK+DA+NLLSLLYDQA+ NAREGRVMIYNLVEAAQEF
Sbjct: 86   SVRCLPGYPYKTPKLQIIPEKGLSKADANNLLSLLYDQASSNAREGRVMIYNLVEAAQEF 145

Query: 587  LSEVAPLEQPH-------------LPRKTVAVSSDRPSK--GPFVYGFLDLFIGSEESWH 721
            LSE+ P E  H             L  K  + SS       GPFVYGFLDLF GS ESWH
Sbjct: 146  LSEIVPQEHLHGSVSCQSTDITSQLTYKDASASSGDTCSFGGPFVYGFLDLFSGSGESWH 205

Query: 722  WGLSVEEXXXXXXXL-----DDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXXX 886
            W L + +       +     + L +     + K++Q +K  + E  K + +  +  K   
Sbjct: 206  WSLGMNDTGRIDSLVQPHVSEGLKHEYDNQQKKIDQIVKPALNEAAKQDSLRKAEMKLDD 265

Query: 887  XXXXXXXXXXXXXXXXXXXXXXXVGEDYPLAXXXXXXXXXXXXXXXH-------SGSASA 1045
                                     ED+ +                        S   S+
Sbjct: 266  LEEESVGESKSCSDLSKSYTDEST-EDHVMCKNIFLEGNISDSGDAQWETEPKPSELVSS 324

Query: 1046 ASIDHHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATES 1225
             S+    + H +EKDLILA LLRLAC  KGPL+DAL ++ SEL  LGIVS RV DLA++ 
Sbjct: 325  GSLVQDHLPHTLEKDLILAHLLRLACGPKGPLSDALPEITSELFDLGIVSKRVQDLASKP 384

Query: 1226 SSLFDKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFG 1405
            SS FD +F++ F    V+S++SQFWK +S+ +GQ++SP  +SRYLNDFEELQPLG GGFG
Sbjct: 385  SSFFDGTFDRVFQAHKVSSKLSQFWKASSEFEGQNSSPPQNSRYLNDFEELQPLGQGGFG 444

Query: 1406 NVVLCKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDA 1585
            +VVLCKNKLDGRQYAVKKIRLKDKS P++DRILREVATLSRLQHQHVVRYYQAWFETG  
Sbjct: 445  HVVLCKNKLDGRQYAVKKIRLKDKSLPVNDRILREVATLSRLQHQHVVRYYQAWFETGIT 504

Query: 1586 GCYGDATWGSRAAASSSFSYLDQSSTDVIGQDNNVG-TYLYIQMEYCPRTLRQMFESYQ- 1759
                D++ GSR   SSSF+Y+D S +D +GQ+N +  TYLYIQMEYCPRTLRQMFESY  
Sbjct: 505  LSCDDSSCGSRTVVSSSFTYVDGSMSDHLGQENKLELTYLYIQMEYCPRTLRQMFESYSN 564

Query: 1760 FEKKPAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLE 1939
             +K+ AWHLFRQIVEGLAHIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQL+
Sbjct: 565  LDKELAWHLFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLD 624

Query: 1940 PDPDPTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGM 2119
             D D  E  G+S+DGTGQ+GTYFYTAPEIEQRWPKINEKADMYSLG++F ELWHPF T M
Sbjct: 625  QDVDAAEMIGVSVDGTGQVGTYFYTAPEIEQRWPKINEKADMYSLGVVFFELWHPFDTAM 684

Query: 2120 ERHVVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESEL 2299
            ERH+VLSDLK+KGE+P  WAAEFPEQA                A ELL++ FPPRME E+
Sbjct: 685  ERHIVLSDLKQKGEVPPAWAAEFPEQASLLRRLMTPSPSDRPSAVELLQNDFPPRMEYEM 744

Query: 2300 LDNILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTE 2479
            LDNILRT+H S+DT +YDK+V A+F+E+ +NTK  +   E  K+  ++ S I  TDL TE
Sbjct: 745  LDNILRTVHISDDTGVYDKIVNAIFNEDTLNTKGHNTNLESSKVVGRDTSCILFTDLQTE 804

Query: 2480 IRDLVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQLN---RNAVKLLTDGGDMIELCHELR 2650
             RD V E+S+ VFR HCAK LEIIPM +LG+  Q N   R+AVKLLT GGD++ELCHELR
Sbjct: 805  RRDNVVEISTEVFRRHCAKHLEIIPMRMLGECPQENSRERSAVKLLTHGGDVVELCHELR 864

Query: 2651 LPFINWVVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAM 2830
            LPF+ W++ N++S FKR+EI YVYRR IGHSPPNRYLQGDFD+IGG +ALTE+E+IKA M
Sbjct: 865  LPFVKWIIANRRSFFKRYEISYVYRRAIGHSPPNRYLQGDFDIIGGETALTESEIIKATM 924

Query: 2831 DIITRFFLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMW 3010
            DII  +F  ESCDIHLNH DLL+AIW+W GI+ +HRQKVAELLSLLGSLRPQS+ERK+ W
Sbjct: 925  DIILHYFHSESCDIHLNHADLLDAIWAWAGIRPEHRQKVAELLSLLGSLRPQSSERKTKW 984

Query: 3011 VVIRRQLRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQ 3190
            VVIRRQLRQELN+ E  VN+LQ VGLRFCG+ADHALPRLRGAL    D    KAL++LS+
Sbjct: 985  VVIRRQLRQELNLAETAVNRLQTVGLRFCGVADHALPRLRGALP--PDKTTRKALDDLSE 1042

Query: 3191 ILMYLRIWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYL 3370
            +  YLR+W++D+ V+ID L+PPTE YHR  FFQI+LRK + +G+  EGTLLAVGGRYDYL
Sbjct: 1043 LFNYLRVWRLDQRVYIDALMPPTESYHRKLFFQIYLRKDDHLGSLMEGTLLAVGGRYDYL 1102

Query: 3371 LHQMWQSEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKR 3550
            LHQ   SE KSNPP A G+SLALETI+QH+S    RP+R D   +VLVCSRGGGGLL +R
Sbjct: 1103 LHQSGDSECKSNPPGAAGSSLALETILQHASSLEVRPYRYDVVTNVLVCSRGGGGLLIER 1162

Query: 3551 MELVAELWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLEL 3712
            MEL+AELWE++I+AEFVP+ DPS  EQY+YA EHDIKCL+ +++       SVK+RHLEL
Sbjct: 1163 MELLAELWEENIRAEFVPLCDPSLTEQYEYANEHDIKCLVIITDTGVSQKDSVKVRHLEL 1222

Query: 3713 KREKEVSRESLVKFLSEAMASQFRNPSIWN 3802
            K+EKEV R +LVKFL EAM+SQFRNPSIWN
Sbjct: 1223 KKEKEVERGNLVKFLLEAMSSQFRNPSIWN 1252


>ref|XP_024030684.1| eIF-2-alpha kinase GCN2 isoform X1 [Morus notabilis]
          Length = 1247

 Score = 1459 bits (3777), Expect = 0.0
 Identities = 767/1235 (62%), Positives = 911/1235 (73%), Gaps = 45/1235 (3%)
 Frame = +2

Query: 233  PATDHTSL----DNELLSEEITALCAIFQEDCDVIS--ENRIKIKLRPYSQDSGYEDLDV 394
            P  DH S     DNELLSEEITALCAIFQEDC  +     ++ IKLRPYS+D G+ED+DV
Sbjct: 23   PLKDHASHESADDNELLSEEITALCAIFQEDCKFLPGPPPQVFIKLRPYSKDMGFEDVDV 82

Query: 395  SALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEA 574
            SA+L +R L GYPYKCPKLQI  EKGLS++DAD LLSLL+DQAN NAREGRVMI+NLVEA
Sbjct: 83   SAVLLVRCLPGYPYKCPKLQITPEKGLSENDADKLLSLLHDQANSNAREGRVMIFNLVEA 142

Query: 575  AQEFLSEVAPLEQPHLPRKTVAVSSDRPSK--------------GPFVYGFLDLFIGSEE 712
            AQEFLSE+ P+ Q       ++ S D  SK              GPFVYGF+DLF GS E
Sbjct: 143  AQEFLSEIVPVGQSQ--ELVLSSSMDSGSKLLYQDNAISTSNLGGPFVYGFVDLFSGSGE 200

Query: 713  SWHWGLSVEEXXXXXXXLD----DLPNGSQPSRDKVNQN-MKMMVVEDNKLNKVLTSTGK 877
            SW+WG  ++E       +     D P       +K  QN +  +V ++ + + +L+   K
Sbjct: 201  SWNWGFGLDENSVIKTSVPARTVDGPKTKNKVEEKHLQNYVGPLVFQETRKDSLLSPMTK 260

Query: 878  XXXXXXXXXXXXXXXXXXXXXXXXXX--VG-------EDYPLAXXXXXXXXXXXXXXXHS 1030
                                        VG       ED+ L                 S
Sbjct: 261  LRTLEEESEEGDKNLSSSNSSTSSVEKSVGNSSTGGEEDFSLVEHIEEDDKGSEC----S 316

Query: 1031 GSASAASIDHHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVAD 1210
             S S+AS+ H ++    +KDLI+  LLRLAC+S   L++A+  +++EL +LGI S++  +
Sbjct: 317  NSLSSASLSHERVPQTSQKDLIMVHLLRLACTSNVALSNAMQQISTELYNLGIFSEQAKE 376

Query: 1211 LATESSSLFDKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLG 1390
            LA+E+S++F+++F+ AF    V+S++S+FWK  SD +G  TS   +SRYLNDFEELQ LG
Sbjct: 377  LASEASTVFNRTFDNAFRQYMVSSKLSEFWKYTSDIEGPRTSLP-NSRYLNDFEELQSLG 435

Query: 1391 HGGFGNVVLCKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWF 1570
            HGGFG+VVLCKNK+DGRQYAVKKIRLKDKS PL+DRILREVATLSRLQHQHVVRYYQAWF
Sbjct: 436  HGGFGHVVLCKNKIDGRQYAVKKIRLKDKSLPLNDRILREVATLSRLQHQHVVRYYQAWF 495

Query: 1571 ETGDAGCYGDATWGSRAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMF 1747
            ETG +G YGDA WGSR A  S+FS+   SS D IGQDN +  TYLYIQMEYCPRTLRQ+F
Sbjct: 496  ETGISGSYGDAIWGSRTAGGSNFSFTGTSSADTIGQDNKLESTYLYIQMEYCPRTLRQVF 555

Query: 1748 ESYQ-FEKKPAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK 1924
            + Y  F+K+ AWH FRQIVEGLAHIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK
Sbjct: 556  DPYSHFDKELAWHFFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLK 615

Query: 1925 LEQLEPDPD-PTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWH 2101
            LEQLE D   P ETTG+S+DGTGQ+GTYFYTAPEIEQ WPKI+EKADMYSLGI+F ELWH
Sbjct: 616  LEQLEQDLSYPAETTGVSVDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWH 675

Query: 2102 PFSTGMERHVVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPP 2281
            PF T MERHVVLSDLK+KGELPS W AEFPEQA                A ELL HAFPP
Sbjct: 676  PFGTAMERHVVLSDLKQKGELPSAWVAEFPEQASLLRRLMSPSPSDRPSAAELLHHAFPP 735

Query: 2282 RMESELLDNILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQH 2461
            RMESELLDNILRTM  SED++IYDKVV A+F EE+++ K+Q N +   +L     S+IQ+
Sbjct: 736  RMESELLDNILRTMQTSEDSNIYDKVVNAIFDEEVLSIKDQHNHTGRFRLPGGGTSAIQY 795

Query: 2462 TDLDTEIRDLVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCH 2641
             +LDTEIRD V E+S+ VFR HCA+ LEII M L  D  Q NRN VKLL+ GGDM+ELCH
Sbjct: 796  AELDTEIRDYVTEISNEVFRKHCARHLEIISMRLSDDCPQFNRNTVKLLSHGGDMLELCH 855

Query: 2642 ELRLPFINWVVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIK 2821
            ELRLPF++WV+ +QKSSFKRFEI  VYRR IGHSPPNRYLQGDFD+IGGASA+TEAE+IK
Sbjct: 856  ELRLPFVSWVIYSQKSSFKRFEISCVYRRAIGHSPPNRYLQGDFDIIGGASAITEAEIIK 915

Query: 2822 AAMDIITRFFLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERK 3001
              MDI+  F  P  C++HLNHG+LLE+IWSW G+KA+HR KVAELLS++ SLRPQSTERK
Sbjct: 916  VTMDILIHFLNPHCCELHLNHGNLLESIWSWTGVKAEHRHKVAELLSMMDSLRPQSTERK 975

Query: 3002 SMWVVIRRQLRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEE 3181
            S WVVIRRQL QELN+ E VVN+LQ VGLRFCG AD ALPRLRGAL    D    KAL+E
Sbjct: 976  SKWVVIRRQLLQELNLPEAVVNRLQTVGLRFCGPADQALPRLRGALPS--DKATRKALDE 1033

Query: 3182 LSQILMYLRIWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRY 3361
            LS +  YLR+W+I+++V+IDPL+PPTE YHR+ FFQ++L K N  GT  +G LLA+GGRY
Sbjct: 1034 LSDLCSYLRVWRIERHVYIDPLMPPTEIYHRELFFQVYLMKENLSGTLVDGILLAIGGRY 1093

Query: 3362 DYLLHQMWQSEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLL 3541
            DYLLHQMW  EHKS+ P  VGTSLALETIIQHS VD+ +P RND S S+LVCSRGGGGLL
Sbjct: 1094 DYLLHQMWVQEHKSHAPGGVGTSLALETIIQHSLVDL-KPIRNDDSVSILVCSRGGGGLL 1152

Query: 3542 EKRMELVAELWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVK--I 3697
            E+ MELVAELWE++IKAEFVP  DPS  EQY+YA EHDIKCL+ + +      GSVK  +
Sbjct: 1153 EEWMELVAELWEENIKAEFVPKPDPSLTEQYEYASEHDIKCLVIIRDTGVAHMGSVKLQV 1212

Query: 3698 RHLELKREKEVSRESLVKFLSEAMASQFRNPSIWN 3802
            RHLE+K+EKEV RE+LVKFL +AMA QFRNPSIWN
Sbjct: 1213 RHLEIKKEKEVERENLVKFLLDAMAVQFRNPSIWN 1247


>dbj|GAV69857.1| Pkinase domain-containing protein/RWD domain-containing
            protein/HGTP_anticodon2 domain-containing
            protein/tRNA-synt_His domain-containing protein
            [Cephalotus follicularis]
          Length = 1246

 Score = 1457 bits (3772), Expect = 0.0
 Identities = 764/1223 (62%), Positives = 906/1223 (74%), Gaps = 34/1223 (2%)
 Frame = +2

Query: 233  PATDH----TSLDNELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDV 394
            P+ DH       DNELLSEEITALCAIFQEDC ++S +  +I IK+RP+S+D GYEDLDV
Sbjct: 28   PSKDHYVSQADDDNELLSEEITALCAIFQEDCKIVSGSPPQIIIKIRPHSKDMGYEDLDV 87

Query: 395  SALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEA 574
            SALL +R L GYPYKCPKLQI  E GL++SDADNLLSLL++QA  N+REGRVMI+NLVEA
Sbjct: 88   SALLVVRCLPGYPYKCPKLQITPEIGLTESDADNLLSLLHEQAISNSREGRVMIFNLVEA 147

Query: 575  AQEFLSEVAPLEQPHLPRKTVA-----------VSSDRP--SKGPFVYGFLDLFIGSEES 715
            AQEFLSE+ P  Q H    ++            VSS++    KGPFV GF+DLF GS +S
Sbjct: 148  AQEFLSEIVPAGQLHESVSSMKNSNGQLFQDTEVSSNKSFCPKGPFVCGFIDLFCGSGDS 207

Query: 716  WHWGLSVEEXXXXXXXLD----DLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXX 883
            WHW L V+        +     D  N     ++K+++N K   +E      + + T K  
Sbjct: 208  WHWSLGVDNNRGTTSSVQSHRLDSSNLGYKVQEKLDKNAKQPAMEGTNQGLLHSPTAKLD 267

Query: 884  XXXXXXXXXXXXXXXXXXXXXXXXV--GEDYPLAXXXXXXXXXXXXXXXHSGSASAASID 1057
                                       G++                    S S S+AS  
Sbjct: 268  TVEEESEDDDTGTSTVDSSGTLVEELEGKEDTFLEQCRTEDDDDDDESEPSESLSSASFG 327

Query: 1058 HHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATESSSLF 1237
            H +    +EKDL++  LLRLACSSKGPL+D L  +A+EL +LGI S+RV DLA++ SSLF
Sbjct: 328  HEKTPQAIEKDLVMVHLLRLACSSKGPLSDVLPPIATELHNLGIFSERVRDLASKPSSLF 387

Query: 1238 DKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGFGNVVL 1417
            +++F+  F  + V+S ISQFWK ASD  G  TS   SSRYLNDFEE+Q LGHGGFG+VVL
Sbjct: 388  NRTFDHVFQQQMVSSMISQFWKPASDFGGPSTSLP-SSRYLNDFEEIQSLGHGGFGHVVL 446

Query: 1418 CKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGDAGCYG 1597
            CKNKLDGRQYAVKKIRLKDK  P++DRILREVATLSRLQHQHVVRYYQAWFETG AG +G
Sbjct: 447  CKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWFETGVAGSFG 506

Query: 1598 DATWGSRAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMFESYQ-FEKK 1771
            D   GS  AASS+FSY   SS D  GQ+N +  T+LYIQME+CPRTLRQ+FESY  F+K+
Sbjct: 507  DTPLGSVTAASSTFSYKGASSADDPGQENKLESTFLYIQMEFCPRTLRQLFESYNHFDKE 566

Query: 1772 PAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLEPDPD 1951
             AWHLF QIVEGLAHIH QGIIHRDLTP+NIFFD+RNDIKIGDFGLAKFLKLEQL+ D  
Sbjct: 567  LAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDSRNDIKIGDFGLAKFLKLEQLDQDGG 626

Query: 1952 -PTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFSTGMERH 2128
             PT+TTG+S+DGTGQ+GTYFYTAPEIEQ WPKI+EKADMYS+G++F ELWHPF T MERH
Sbjct: 627  FPTDTTGVSVDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSVGVVFFELWHPFGTAMERH 686

Query: 2129 VVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMESELLDN 2308
            VVLSDLK KGELP+ W AEFPEQA                ATELL++AFPPRME ELLDN
Sbjct: 687  VVLSDLKLKGELPAAWVAEFPEQASLLRRLMSPSPSDRPSATELLQYAFPPRMEYELLDN 746

Query: 2309 ILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLDTEIRD 2488
            ILRTM +SEDTS+YDKVV A+F EE++  KN        ++ A +  SIQ+TDL TE+RD
Sbjct: 747  ILRTMQSSEDTSVYDKVVNAIFDEEMLG-KNHHQPVGRLRMAAHDTLSIQYTDLGTELRD 805

Query: 2489 LVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRLPFINW 2668
             V EV+  V+RLHCAK LEIIPM LL D  Q NRN VKLLT GGDMIELCHELRLPF++W
Sbjct: 806  YVVEVTREVYRLHCAKHLEIIPMRLLDDCSQFNRNTVKLLTHGGDMIELCHELRLPFVSW 865

Query: 2669 VVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMDIITRF 2848
            ++ NQKSSFKR+EI YVYR+ IGHSPPNRYLQGDFD++GGASALTEAEVIK  MD++  F
Sbjct: 866  LIANQKSSFKRYEISYVYRKAIGHSPPNRYLQGDFDIVGGASALTEAEVIKVTMDVVNCF 925

Query: 2849 FLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWVVIRRQ 3028
            F  +SCD+HLNHG LL+AIWSW GIKA+H  KVAE LSL+GSLRPQS+ERK  WVVIRRQ
Sbjct: 926  FHSDSCDVHLNHGGLLDAIWSWAGIKAEHVHKVAERLSLMGSLRPQSSERKLKWVVIRRQ 985

Query: 3029 LRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQILMYLR 3208
            L QELN+ E VVN+LQ VGLRFCG AD ALPRLRGAL    D    KAL+ELS +  YLR
Sbjct: 986  LLQELNLAEAVVNRLQTVGLRFCGAADQALPRLRGALP--ADRATRKALDELSDLFSYLR 1043

Query: 3209 IWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLLHQMWQ 3388
            +WKI+K+V+ID L+ PTE YHRD FFQI+L K N  G+  EG LLAVGGRYDYLLHQMW 
Sbjct: 1044 VWKIEKHVYIDSLMAPTESYHRDLFFQIYLTKENQPGSLFEGALLAVGGRYDYLLHQMWN 1103

Query: 3389 SEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRMELVAE 3568
             E+K+N P AVG+SLALET+IQHS  D  +P RN+ + ++LVCSRGGGGLL +RMELVAE
Sbjct: 1104 QEYKTNSPGAVGSSLALETLIQHSLGDY-KPIRNEGNTNILVCSRGGGGLLVERMELVAE 1162

Query: 3569 LWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELKREKEV 3730
            LWE++IKAEFVPI DPS  EQY+YA EHDIKCL+ +++      GSVK+RHLELK+EKEV
Sbjct: 1163 LWEENIKAEFVPIPDPSLTEQYEYANEHDIKCLVIITDTGVSQTGSVKVRHLELKKEKEV 1222

Query: 3731 SRESLVKFLSEAMASQFRNPSIW 3799
             RE++V+FL EAMA+QFRNP +W
Sbjct: 1223 ERENIVRFLLEAMATQFRNPLVW 1245


>ref|XP_018806250.1| PREDICTED: eIF-2-alpha kinase GCN2 isoform X2 [Juglans regia]
          Length = 1244

 Score = 1454 bits (3763), Expect = 0.0
 Identities = 766/1229 (62%), Positives = 907/1229 (73%), Gaps = 39/1229 (3%)
 Frame = +2

Query: 233  PATDHTSL----DNELLSEEITALCAIFQEDCDVI--SENRIKIKLRPYSQDSGYEDLDV 394
            P  D+TS     DNELLSEEITALCAIFQEDC +I  S  +I IKLRPYS+D GYEDL+V
Sbjct: 23   PLKDNTSHLGGDDNELLSEEITALCAIFQEDCKIIPGSPPQIIIKLRPYSKDMGYEDLNV 82

Query: 395  SALLSIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEA 574
            SALL +R L GYPYKCPKLQI  E GLSKSDAD LL+LL DQAN NAREGRVMI+NLVEA
Sbjct: 83   SALLLVRCLPGYPYKCPKLQITPENGLSKSDADKLLALLSDQANSNAREGRVMIFNLVEA 142

Query: 575  AQEFLSEVAPLEQPHLP------------RKTVAVSSDRPSKGPFVYGFLDLFIGSEESW 718
            AQEFLSE  P+ Q H               K +  SS++  +GPFVYG +DLF GS ESW
Sbjct: 143  AQEFLSETVPVGQSHESVACSTMDGGGEFLKEITASSNK--RGPFVYGLMDLFSGSGESW 200

Query: 719  HWGLSVEEXXXXXXX-----LDDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKXX 883
            +WG  ++E            L+    G    +  +++N+K   ++D K   +   T K  
Sbjct: 201  NWGFEMDEKSGVNSSVQPHTLEGSKLGYDYQQKGLDKNLKPSKIQDTKQGLLPFPTAKLD 260

Query: 884  XXXXXXXXXXXXXXXXXXXXXXXXVG-------EDYPLAXXXXXXXXXXXXXXXHSGSAS 1042
                                    +         D+                   SGS  
Sbjct: 261  TLDEETEDSNKSISSTDSSGFPEELAGDATDDERDFMEMEKTTEDDGDGEFSSEASGSLP 320

Query: 1043 AASIDHHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLATE 1222
            +AS+ + + S  VE+DLI+  LLRLAC+S+GPL +AL  + +EL S GI+S+   DLA++
Sbjct: 321  SASVGNGKESQMVERDLIMVHLLRLACASRGPLDEALPQITTELYSQGIISEWARDLASK 380

Query: 1223 SSSLFDKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGGF 1402
             SS+F+K+F+  F  + V+SRISQFWK  +D +   TS   SSR+L+DFEEL+PLGHGGF
Sbjct: 381  PSSIFNKTFDHVFQKQMVSSRISQFWKPTTDFEDPSTSLP-SSRFLSDFEELKPLGHGGF 439

Query: 1403 GNVVLCKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETGD 1582
            G+VVLCKNKLDGRQYAVKKIRLKDK  P++DRILREVATLSRLQHQHVVRYYQAWFETG 
Sbjct: 440  GHVVLCKNKLDGRQYAVKKIRLKDKILPVNDRILREVATLSRLQHQHVVRYYQAWFETGV 499

Query: 1583 AGCYGDATWGSRAAASSSFSYLDQSSTDVIGQDNNV-GTYLYIQMEYCPRTLRQMFESYQ 1759
               +GD T GS  AASSSFSYL  SS D +G +N +  TYLYIQMEYCPRTLRQ+FESY 
Sbjct: 500  VSSFGDTTGGSMTAASSSFSYLGTSSVDALGHENKLESTYLYIQMEYCPRTLRQVFESYS 559

Query: 1760 -FEKKPAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQL 1936
             F+K+ AWHLFRQIVEGLAHIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQL
Sbjct: 560  HFDKELAWHLFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQL 619

Query: 1937 EPDPD-PTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFST 2113
            + DP  P++T G+S+DGTGQ+GTYFYTAPEIEQ WPKI+EKADMYSLG++F ELWHPF T
Sbjct: 620  DQDPGYPSDTAGVSLDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGVVFFELWHPFGT 679

Query: 2114 GMERHVVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMES 2293
             MERH+VL+DLK+KGELP  W AEFPEQ                 ATELL+HAFPPRMES
Sbjct: 680  AMERHIVLTDLKQKGELPPSWIAEFPEQESLLRRLMSPSPSDRPSATELLQHAFPPRMES 739

Query: 2294 ELLDNILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLD 2473
            ELLDNILRTM  SED S+YDKVV A+F E++++ K+    +   +L   E +SIQ+TDLD
Sbjct: 740  ELLDNILRTMQTSEDRSMYDKVVNAIFDEDMLSIKDVHRHAGRLRLAGDE-TSIQYTDLD 798

Query: 2474 TEIRDLVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQLNRNAVKLLTDGGDMIELCHELRL 2653
            TE+RD V EV+  VFR HCAK LEI+PM LL D  Q NRN VKLLT GGD +ELC ELRL
Sbjct: 799  TEVRDYVVEVTREVFRQHCAKHLEIVPMRLLDDFPQFNRNTVKLLTHGGDKLELCRELRL 858

Query: 2654 PFINWVVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKAAMD 2833
            PF++WV+ +QKSSFKR+EI  VYRR IGHSPPNRYLQGDFDVIGGASALTEAEVIK  MD
Sbjct: 859  PFVSWVISHQKSSFKRYEISCVYRRAIGHSPPNRYLQGDFDVIGGASALTEAEVIKVTMD 918

Query: 2834 IITRFFLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKSMWV 3013
            II +FF  +SCDIHLNHGDLL+AIWSWIG+KA+HRQKVAELL ++GSLRPQS+ERKS WV
Sbjct: 919  IIAQFFSADSCDIHLNHGDLLDAIWSWIGVKAEHRQKVAELLLMMGSLRPQSSERKSKWV 978

Query: 3014 VIRRQLRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEELSQI 3193
            VIRRQL QELN+ E VVN+LQ V LRFCG AD ALPRLRGAL    D    KAL+ELS +
Sbjct: 979  VIRRQLLQELNLAEAVVNRLQTVSLRFCGAADQALPRLRGALP--ADKPTRKALDELSDL 1036

Query: 3194 LMYLRIWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYDYLL 3373
              YLRIW+I+K+V+ID L+PPTE YHRD FFQ++L K N+ G+  EG LLA GG Y+YLL
Sbjct: 1037 CSYLRIWRIEKHVYIDALMPPTESYHRDLFFQVYLIKENNPGSLIEGALLAAGGHYNYLL 1096

Query: 3374 HQMWQSEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLEKRM 3553
            HQ+W  E+K++PP AVGTSLALETIIQ SS+D  +P R +SS  +LVCSRGGGGLL +RM
Sbjct: 1097 HQLWGHEYKTSPPGAVGTSLALETIIQRSSIDF-KPTRIESSTIILVCSRGGGGLLVERM 1155

Query: 3554 ELVAELWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHLELK 3715
            ELVAELWE++IKAEFVP  DPS  EQY+YA EHDIKCL+ +++      GSVK+RHLELK
Sbjct: 1156 ELVAELWEENIKAEFVPAPDPSLTEQYEYANEHDIKCLVILTDTDVSQLGSVKVRHLELK 1215

Query: 3716 REKEVSRESLVKFLSEAMASQFRNPSIWN 3802
            +EK+V RE LV+FL  AM +QFRNPSIW+
Sbjct: 1216 KEKKVEREILVRFLLAAMTTQFRNPSIWS 1244


>ref|NP_001311633.1| eIF-2-alpha kinase GCN2 [Nicotiana tabacum]
 ref|XP_016434811.1| PREDICTED: eIF-2-alpha kinase GCN2 [Nicotiana tabacum]
 gb|AJO15929.1| protein kinase family protein GCN2 [Nicotiana tabacum]
          Length = 1252

 Score = 1451 bits (3757), Expect = 0.0
 Identities = 759/1232 (61%), Positives = 913/1232 (74%), Gaps = 45/1232 (3%)
 Frame = +2

Query: 242  DHTSL---DNELLSEEITALCAIFQEDCDVISEN--RIKIKLRPYSQDSGYEDLDVSALL 406
            DH S+   ++ELL+EE+TALCAIFQEDC+V+SE+  ++ I+LRPYS+D+GYED DVSA+L
Sbjct: 26   DHNSIGDGNSELLAEELTALCAIFQEDCEVVSESPSQLHIQLRPYSKDAGYEDSDVSAIL 85

Query: 407  SIRFLDGYPYKCPKLQIELEKGLSKSDADNLLSLLYDQANLNAREGRVMIYNLVEAAQEF 586
            S+R L GYPYK PKLQI  EKGLSK+DA+NLLSLLYDQA+ NAREGRVMIYNLVEAAQEF
Sbjct: 86   SVRCLPGYPYKTPKLQIIPEKGLSKADANNLLSLLYDQASSNAREGRVMIYNLVEAAQEF 145

Query: 587  LSEVAPLEQPHLPRKTVAVSSDRPSK-----------------GPFVYGFLDLFIGSEES 715
            LSE+ P  Q HL       S+D  S+                 G FVYGFLDLF GS ES
Sbjct: 146  LSEIVP--QEHLHGSVSCQSTDITSQFTYKDATASSGDTCSFGGQFVYGFLDLFSGSGES 203

Query: 716  WHWGLSVEEXXXXXXXL-----DDLPNGSQPSRDKVNQNMKMMVVEDNKLNKVLTSTGKX 880
            WHW L + +       +     + L +     + K++Q +K  + E  K + +  +  K 
Sbjct: 204  WHWSLGMNDTGCIDSLVQSHVSEGLTHEYDNQQKKIDQIVKPALNEAAKQDSLRKAEMKL 263

Query: 881  XXXXXXXXXXXXXXXXXXXXXXXXXVGEDYPLAXXXXXXXXXXXXXXXH-------SGSA 1039
                                       ED+ +                        S   
Sbjct: 264  DDLEEESVGESKSCSDLSKSYTDEST-EDHVMCKNIFLEGNISDSGDAQRETEPKPSELV 322

Query: 1040 SAASIDHHQISHDVEKDLILAQLLRLACSSKGPLADALTDVASELASLGIVSDRVADLAT 1219
            S+ S+    + H +EKDLILA LLRLAC +KGPL+DAL +V SEL  LGIVS RV DLA+
Sbjct: 323  SSGSLVLDHLPHTLEKDLILAHLLRLACGAKGPLSDALPEVTSELFDLGIVSKRVQDLAS 382

Query: 1220 ESSSLFDKSFNQAFGHRTVTSRISQFWKTASDTKGQHTSPAFSSRYLNDFEELQPLGHGG 1399
            + SS+FD +F++ F    V+S++SQFWK +S+ +GQ++SP  +SRYLNDFEELQPLG GG
Sbjct: 383  KPSSIFDGTFDRVFQAHKVSSKLSQFWKASSEFEGQNSSPPQNSRYLNDFEELQPLGQGG 442

Query: 1400 FGNVVLCKNKLDGRQYAVKKIRLKDKSQPLDDRILREVATLSRLQHQHVVRYYQAWFETG 1579
            FG+VVLCKNKLDGRQYAVKKIRLKDKS PL+DRILREVATLSRLQHQHVVRYYQAWFETG
Sbjct: 443  FGHVVLCKNKLDGRQYAVKKIRLKDKSLPLNDRILREVATLSRLQHQHVVRYYQAWFETG 502

Query: 1580 DAGCYGDATWGSRAAASSSFSYLDQSSTDVIGQDNNVG-TYLYIQMEYCPRTLRQMFESY 1756
                  D++ GSR   SSSF+Y+D S +D +GQ+N +  TYLYIQMEYCPRTLRQMFESY
Sbjct: 503  ITLSCDDSSCGSRTVVSSSFTYVDGSMSDHLGQENKLELTYLYIQMEYCPRTLRQMFESY 562

Query: 1757 -QFEKKPAWHLFRQIVEGLAHIHSQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQ 1933
               +K+ AWHLFRQIVEGLAHIH QGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQ
Sbjct: 563  SNLDKELAWHLFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQ 622

Query: 1934 LEPDPDPTETTGMSIDGTGQIGTYFYTAPEIEQRWPKINEKADMYSLGIIFLELWHPFST 2113
            L+ D D  E  G+S+DGTGQ+GTYFYTAPEIEQRWPKINEKADMYSLG++F ELWHPF T
Sbjct: 623  LDQDVDAAEMIGVSVDGTGQVGTYFYTAPEIEQRWPKINEKADMYSLGVVFFELWHPFDT 682

Query: 2114 GMERHVVLSDLKKKGELPSDWAAEFPEQAXXXXXXXXXXXXXXXXATELLKHAFPPRMES 2293
             MERH+VLSDLK+KGE+P  WAAEFPEQA                A ELL++ FPPRME 
Sbjct: 683  AMERHIVLSDLKQKGEVPPAWAAEFPEQASLLRRLMTPSPSDRPSAVELLQNDFPPRMEY 742

Query: 2294 ELLDNILRTMHNSEDTSIYDKVVGAVFSEEIINTKNQDNESEVPKLGAKENSSIQHTDLD 2473
            E+LDNILRT+H S+DT +YDK+V A+F+E+ +NTK  +   E  K+  ++ S I  TDL 
Sbjct: 743  EMLDNILRTVHISDDTGVYDKIVNAIFNEDTLNTKGHNTNLESSKVIGRDTSCILFTDLQ 802

Query: 2474 TEIRDLVWEVSSTVFRLHCAKRLEIIPMCLLGDSLQLN---RNAVKLLTDGGDMIELCHE 2644
            TE RD V E+S+ VFR HCAK L IIPM +LG+  Q N   R+AVKLLT GGD++ELCHE
Sbjct: 803  TERRDNVVEISTEVFRRHCAKHLGIIPMRMLGECPQANSRERSAVKLLTHGGDVVELCHE 862

Query: 2645 LRLPFINWVVLNQKSSFKRFEICYVYRRPIGHSPPNRYLQGDFDVIGGASALTEAEVIKA 2824
            LRLPF+ W++ N++S FKR+EI YVYRR IGHSPPNRYLQGDFD+IGG +ALTE+E+IKA
Sbjct: 863  LRLPFVKWIIANRRSFFKRYEISYVYRRAIGHSPPNRYLQGDFDIIGGETALTESEIIKA 922

Query: 2825 AMDIITRFFLPESCDIHLNHGDLLEAIWSWIGIKADHRQKVAELLSLLGSLRPQSTERKS 3004
             MDII  +F  ESCDIHLNH DLL+AIW+W GI+ +HRQKVAELLSLLGSLRPQS+ERK+
Sbjct: 923  TMDIILHYFHSESCDIHLNHADLLDAIWAWAGIRPEHRQKVAELLSLLGSLRPQSSERKT 982

Query: 3005 MWVVIRRQLRQELNITEGVVNKLQNVGLRFCGIADHALPRLRGALQGCVDTLAMKALEEL 3184
             WVVIRRQLRQELN+ E  VN+LQ VGLRFCG+ADHALPRLRGAL    D    KALE+L
Sbjct: 983  KWVVIRRQLRQELNLAETAVNRLQTVGLRFCGVADHALPRLRGALP--PDKTTRKALEDL 1040

Query: 3185 SQILMYLRIWKIDKNVFIDPLIPPTEGYHRDSFFQIFLRKYNSVGTPTEGTLLAVGGRYD 3364
            S++  YLR+W++D+ V+ID L+PPTE YHR  FFQI+LRK + +G+  EGTLLAVGGRYD
Sbjct: 1041 SELFNYLRVWRLDQRVYIDALMPPTESYHRKLFFQIYLRKDDHLGSLMEGTLLAVGGRYD 1100

Query: 3365 YLLHQMWQSEHKSNPPRAVGTSLALETIIQHSSVDICRPFRNDSSKSVLVCSRGGGGLLE 3544
            YLLHQ   SE KSNP  A G+SLALETI+QH+S    RP+R D   +VLVCSRGGGGLL 
Sbjct: 1101 YLLHQSGDSECKSNPLGAAGSSLALETILQHASSLEFRPYRYDVVTNVLVCSRGGGGLLI 1160

Query: 3545 KRMELVAELWEDDIKAEFVPIVDPSPKEQYDYAKEHDIKCLIEMSE------GSVKIRHL 3706
            +RMEL+AELWE++I+AEFVP+ DPS  EQY+YA EHDIKCL+ +++       SVK+RHL
Sbjct: 1161 ERMELLAELWEENIRAEFVPLCDPSLTEQYEYANEHDIKCLVIITDTCVSQKDSVKVRHL 1220

Query: 3707 ELKREKEVSRESLVKFLSEAMASQFRNPSIWN 3802
            ELK+EKEV R +LVKFL EAM+SQFRNPSIWN
Sbjct: 1221 ELKKEKEVERGNLVKFLLEAMSSQFRNPSIWN 1252