BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00013659
         (622 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AGO03794.1| cytochrome P450-like protein [Tanacetum cinerarii...   334   e-110
gb|KVH98298.1| cytochrome P450 [Cynara cardunculus var. scolymus]     244   3e-75
ref|XP_023741018.1| cytochrome P450 81D1-like [Lactuca sativa] >...   243   8e-75
gb|KVH98297.1| cytochrome P450 [Cynara cardunculus var. scolymus]     240   1e-69
ref|XP_022015673.1| cytochrome P450 81F3-like isoform X2 [Helian...   164   2e-44
ref|XP_022015664.1| cytochrome P450 81F3-like isoform X1 [Helian...   163   4e-44
ref|XP_023535659.1| isoflavone 2'-hydroxylase-like [Cucurbita pe...   155   2e-41
ref|XP_022936848.1| isoflavone 2'-hydroxylase-like [Cucurbita mo...   155   2e-41
ref|XP_022977008.1| isoflavone 2'-hydroxylase-like [Cucurbita ma...   155   3e-41
ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ...   155   3e-41
ref|XP_021691638.1| cytochrome P450 81E8-like [Hevea brasiliensis]    150   6e-41
ref|XP_008452618.1| PREDICTED: cytochrome P450 81E8-like [Cucumi...   155   7e-41
ref|XP_021612036.1| cytochrome P450 81E8-like [Manihot esculenta...   154   1e-40
ref|XP_009354182.2| PREDICTED: cytochrome P450 81D11-like [Pyrus...   152   5e-40
gb|OAY50996.1| hypothetical protein MANES_05G179300 [Manihot esc...   152   6e-40
ref|XP_021612537.1| cytochrome P450 81D1-like [Manihot esculenta]     152   7e-40
ref|XP_008378166.1| PREDICTED: isoflavone 2'-hydroxylase-like [M...   151   9e-40
ref|XP_010654768.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   151   1e-39
ref|XP_024162938.1| cytochrome P450 81D11-like [Rosa chinensis] ...   150   2e-39
ref|XP_004141396.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   150   4e-39

>gb|AGO03794.1| cytochrome P450-like protein [Tanacetum cinerariifolium]
          Length = 509

 Score =  334 bits (857), Expect = e-110
 Identities = 172/212 (81%), Positives = 185/212 (87%), Gaps = 5/212 (2%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D +FA R KLITGKEFGCNYT LASA HG  WRHLRRVS+LEILPFHRL GQDGSIADEV
Sbjct: 92  DKVFAYRPKLITGKEFGCNYTGLASAPHGAHWRHLRRVSSLEILPFHRLPGQDGSIADEV 151

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAAEDDQ-----LKDYAI 277
           KLLLG LF T+KEIVE+KSVFLDLVFD+MMKMFAGKRY EKK+  EDDQ     LKDY I
Sbjct: 152 KLLLGRLFHTEKEIVEMKSVFLDLVFDMMMKMFAGKRYDEKKRTTEDDQIKSTSLKDYVI 211

Query: 276 RSFRITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGS 97
           RSFRITT E DV YFMPILK LGLRGLERKCN+LQK+GDA M+SLIE+VRKKIP+FS GS
Sbjct: 212 RSFRITTGEPDVAYFMPILKSLGLRGLERKCNELQKKGDALMDSLIEDVRKKIPEFSIGS 271

Query: 96  GETKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
           GET+EKVIE LLARQKD PKRYSD+TIRGLLL
Sbjct: 272 GETEEKVIELLLARQKDDPKRYSDETIRGLLL 303


>gb|KVH98298.1| cytochrome P450 [Cynara cardunculus var. scolymus]
          Length = 503

 Score =  244 bits (623), Expect = 3e-75
 Identities = 129/211 (61%), Positives = 158/211 (74%), Gaps = 4/211 (1%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D  FANR KL+ GK FG NYT LA A HG LW HLRRVS LEILPFHRL  Q+ S  +EV
Sbjct: 91  DAAFANRPKLLPGKVFGHNYTNLAWAPHGALWCHLRRVSLLEILPFHRLPAQNESCTEEV 150

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAAED----DQLKDYAIR 274
           +LLLG LF ++++++ELK +FL+LV DVMM+MFAGKRY  KK A  D      L DY  R
Sbjct: 151 RLLLGRLFHSEEKVIELKPMFLNLVLDVMMRMFAGKRYCTKKMAEGDVTQPISLLDYVTR 210

Query: 273 SFRITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSG 94
           SFR+TT E DVGYFMPILK LGLRGLE++CN LQK+GD  M+S+I E++ ++     GSG
Sbjct: 211 SFRMTTGEPDVGYFMPILKLLGLRGLEQRCNDLQKKGDLLMDSIIHELKTRM----LGSG 266

Query: 93  ETKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
           E ++KVIEFLLARQ+D PK Y D+ I+GL+L
Sbjct: 267 EKEDKVIEFLLARQQDDPKLYPDEMIKGLVL 297


>ref|XP_023741018.1| cytochrome P450 81D1-like [Lactuca sativa]
 gb|PLY68301.1| hypothetical protein LSAT_1X23861 [Lactuca sativa]
          Length = 509

 Score =  243 bits (620), Expect = 8e-75
 Identities = 128/211 (60%), Positives = 158/211 (74%), Gaps = 5/211 (2%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D +FA+R KL+ GKEFG NY+ LA A HG  WRHLRRVS LEILPFHRL  Q  S+ DEV
Sbjct: 92  DAVFAHRPKLVVGKEFGSNYSNLAWAPHGGHWRHLRRVSCLEILPFHRLPEQHDSLTDEV 151

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAAEDDQLK-----DYAI 277
           KLLLG LF ++K+ VELK +FLDLV +VMMKMFAG  +  KK   E + ++     DY  
Sbjct: 152 KLLLGRLFHSQKKTVELKPLFLDLVLNVMMKMFAGNGFCPKKMTCEGNGMESISMVDYVT 211

Query: 276 RSFRITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGS 97
            SFR+TT E DVGYFMP+LK LGL  LE++C +LQK+GD+ M+SLIEE+R K+ D   GS
Sbjct: 212 HSFRMTTDEQDVGYFMPLLKTLGLITLEQRCKELQKKGDSLMDSLIEELRSKMRDI-EGS 270

Query: 96  GETKEKVIEFLLARQKDHPKRYSDKTIRGLL 4
           G+ + KVIEFLLARQK++P+RY DK IRGL+
Sbjct: 271 GQKEGKVIEFLLARQKENPERYPDKLIRGLV 301


>gb|KVH98297.1| cytochrome P450 [Cynara cardunculus var. scolymus]
          Length = 2951

 Score =  240 bits (612), Expect = 1e-69
 Identities = 132/217 (60%), Positives = 157/217 (72%), Gaps = 10/217 (4%)
 Frame = -2

Query: 621  DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
            D +FA+R KL+ GK+FG NYT LA A  G LWRHLRRVS LEILPFHRL+    S+ADEV
Sbjct: 569  DAVFAHRPKLLAGKQFGHNYTNLAWAPRGALWRHLRRVSCLEILPFHRLTDHHDSLADEV 628

Query: 441  KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKR----YGEKK-----KAAEDDQLK 289
            KLLLG LF +  EIVELK VFL+LV DVM++MF GK     Y  KK      A E     
Sbjct: 629  KLLLGRLFHSDNEIVELKPVFLELVVDVMIRMFTGKSVCDGYCRKKLTARMNAMESISFL 688

Query: 288  DYAIRSFRITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDF 109
            DY   SFR+TT E D+GYFMPILK LGLRGLE +  +LQ++GD  M++LI E+R K+ +F
Sbjct: 689  DYVTSSFRMTTAEPDLGYFMPILKLLGLRGLEHRMKELQQKGDLLMDNLIGELRTKMLEF 748

Query: 108  SNGSGETK-EKVIEFLLARQKDHPKRYSDKTIRGLLL 1
            S+GSG+ + EKVIEFLLARQKD PK Y D+ IRGLLL
Sbjct: 749  SDGSGDQREEKVIEFLLARQKDDPKGYHDEIIRGLLL 785



 Score =  137 bits (346), Expect = 8e-34
 Identities = 81/210 (38%), Positives = 125/210 (59%), Gaps = 3/210 (1%)
 Frame = -2

Query: 621  DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
            D++FANR +++ GK  G NYT+LA + +G  WR+LRR++T+EIL  HRL+       DE 
Sbjct: 2540 DIIFANRPRMLFGKIIGLNYTSLAWSPYGDNWRNLRRIATVEILSVHRLNEFHDIRVDEG 2599

Query: 441  KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAAEDDQLKDY--AIRSF 268
            KLL+  L       V +KSVF +L  +VMM+M +GKRY      A D++ K +   +   
Sbjct: 2600 KLLIRKLLSASSSPVNMKSVFYELTLNVMMRMISGKRYFGGDIPAVDEEGKRFREILNQT 2659

Query: 267  RITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGET 88
             +     ++  ++PIL +LG++GL+ K   LQ++ D   + LIE++RK       G  E 
Sbjct: 2660 FLLAGAANIVDYLPILGWLGVKGLKNKLIALQEKRDVFFQGLIEQLRKS----KGGESEN 2715

Query: 87   KEK-VIEFLLARQKDHPKRYSDKTIRGLLL 1
            K+K +IE LL+ Q+  P+ Y+D  IR L+L
Sbjct: 2716 KKKTMIEVLLSLQESDPEYYTDAMIRSLIL 2745



 Score =  134 bits (337), Expect = 1e-32
 Identities = 75/207 (36%), Positives = 123/207 (59%)
 Frame = -2

Query: 621  DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
            D+ FANR +++ GK  G NYT LA +S+G  WR+LRR++++EIL  HRL+       +E 
Sbjct: 1562 DITFANRPRMLFGKILGMNYTTLAWSSYGDNWRNLRRIASIEILSIHRLNELQDIRVEEG 1621

Query: 441  KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAAEDDQLKDYAIRSFRI 262
            +LL+  L       V +KSVF +   ++MM+M +GKRY +  K  E+ +     +R   +
Sbjct: 1622 RLLIHKLLSNSSSPVTVKSVFYEHTLNMMMRMISGKRYFDGDKLEEEGKEFKKILRDTFV 1681

Query: 261  TTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGETKE 82
                ++VG ++PIL +LG+  LE+K   L+++ D   + LIE++RK   D     G  ++
Sbjct: 1682 LMGASNVGDYLPILSWLGVNELEKKLIALKERRDVFFQGLIEQLRKGKGD---QVGNKRK 1738

Query: 81   KVIEFLLARQKDHPKRYSDKTIRGLLL 1
             +IE LL+ Q+  P+ Y+D+ IR  +L
Sbjct: 1739 NMIEVLLSLQESDPEYYTDELIRNFVL 1765



 Score =  132 bits (332), Expect = 6e-32
 Identities = 79/208 (37%), Positives = 122/208 (58%), Gaps = 1/208 (0%)
 Frame = -2

Query: 621  DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
            D+ FANR +++ GK  G NYT LA   +G  WR+LRR++++EIL  HRL+       DE 
Sbjct: 1058 DITFANRPRMLFGKIIGLNYTTLAWTPYGDNWRNLRRIASIEILSIHRLNEFHDIRVDEG 1117

Query: 441  KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRY-GEKKKAAEDDQLKDYAIRSFR 265
            +LL+  L       V +KSVF +   +VMM+M +GKRY G  K   E  QL+     SF 
Sbjct: 1118 RLLIRKLLSNYSSPVTVKSVFYEQTLNVMMRMISGKRYFGGDKLEEEGKQLRQILDDSF- 1176

Query: 264  ITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGETK 85
            +    +++G ++PIL +LG++ LE+K   L+++ D   + LIE+VR+   D +    +T 
Sbjct: 1177 VLAGASNIGDYLPILSWLGVKRLEKKLIALKERRDVFFQGLIEQVREGNGDQARNKRKT- 1235

Query: 84   EKVIEFLLARQKDHPKRYSDKTIRGLLL 1
              +IE LL+ Q+  P+ Y+D  IR  +L
Sbjct: 1236 --MIELLLSLQESDPEYYTDALIRNFVL 1261



 Score =  130 bits (326), Expect = 4e-31
 Identities = 75/207 (36%), Positives = 125/207 (60%)
 Frame = -2

Query: 621  DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
            D++FANR +++ GK  G NYT+ +S+S+G  WR+LRR++++EIL  HRL+       +E 
Sbjct: 2066 DIIFANRPRMLFGKIIGQNYTSFSSSSYGDNWRNLRRIASIEILSIHRLNEFHDIRVEEG 2125

Query: 441  KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAAEDDQLKDYAIRSFRI 262
            +LL+  L  +    V +K VF +L  +VMM+M +GKRY       E  +L++    +F +
Sbjct: 2126 RLLIRKLL-SDSSPVNVKLVFYELTLNVMMRMVSGKRYFGGDNLEEGKELREILNDTFML 2184

Query: 261  TTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGETKE 82
             +  ++VG ++PIL  LG+  LE+K   LQ++ D   + LIE++RK   D     G  ++
Sbjct: 2185 AS-ASNVGDYLPILSCLGVNRLEKKLIALQERRDVFFQGLIEQLRKGSRDH---EGNKRK 2240

Query: 81   KVIEFLLARQKDHPKRYSDKTIRGLLL 1
             +I+ LL+ Q+  P+ Y+D  IR  +L
Sbjct: 2241 TMIDLLLSLQESDPEYYTDAMIRNFVL 2267



 Score =  119 bits (297), Expect = 3e-27
 Identities = 68/210 (32%), Positives = 124/210 (59%), Gaps = 3/210 (1%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           DL+FANR K++ GK  G NYT+++ AS+   WR+LRR+++ E+L  H L+       +E 
Sbjct: 93  DLVFANRPKMLYGKIIGNNYTSMSWASYSDHWRNLRRIASTELLSLHHLNELHAIREEEG 152

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAAEDDQLKDY--AIRSF 268
            +++  L  T    V +KSVF +L  +VMM+M +GKRY     A  D++ K +   +   
Sbjct: 153 GVMIHNLLLTSSSPVNMKSVFYELTLNVMMRMISGKRYFSGDIADVDEEGKRFKKLLEEM 212

Query: 267 RITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGET 88
            +++  +++  ++P L +LG++G E+K   LQ+  +   + LI ++++       G  E 
Sbjct: 213 FLSSGASNLADYLPNLSWLGVKGSEKKLIALQENINVFFQGLINQLKQ----VKGGEVEN 268

Query: 87  KEK-VIEFLLARQKDHPKRYSDKTIRGLLL 1
           ++K +IE LL+ Q+ HP+ Y+D+ I+  ++
Sbjct: 269 QKKTMIEVLLSLQRLHPEYYNDEMIKSFVM 298


>ref|XP_022015673.1| cytochrome P450 81F3-like isoform X2 [Helianthus annuus]
          Length = 496

 Score =  164 bits (414), Expect = 2e-44
 Identities = 93/213 (43%), Positives = 136/213 (63%), Gaps = 6/213 (2%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRR-VSTLEILPFHRLSGQDGSIADE 445
           D++FANR KL+ GK FGCN+T LA AS+   W+HLR  VS  E     RL      +ADE
Sbjct: 90  DVVFANRPKLVPGKVFGCNFTNLAWASYDSHWKHLRSCVSCFEKQQSQRLPDL---LADE 146

Query: 444 VKLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAAEDDQ-----LKDYA 280
           VKLL+  L     + ++LK +F +LVF+VMM+M+AGKR    K   +  +     L +YA
Sbjct: 147 VKLLIRQLCCNNNDAIQLKPMFQELVFNVMMRMYAGKRSCRYKTTGQQKENELISLLEYA 206

Query: 279 IRSFRITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNG 100
           I SF++TT E+D+ YF PILK LG+ GLE++C +LQ++GD+ + +LI ++R+K       
Sbjct: 207 IHSFKMTTAESDLTYFRPILKLLGVTGLEQRCTQLQRKGDSLIHNLINKIREK------- 259

Query: 99  SGETKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
               +E +I+FLL +QK+ P  Y+ + I GL+L
Sbjct: 260 -SLKEESLIQFLLDKQKEDPLNYTKEIISGLVL 291


>ref|XP_022015664.1| cytochrome P450 81F3-like isoform X1 [Helianthus annuus]
 gb|OTG33928.1| putative cytochrome P450, family 81, subfamily F, polypeptide 3
           [Helianthus annuus]
          Length = 500

 Score =  163 bits (412), Expect = 4e-44
 Identities = 93/217 (42%), Positives = 138/217 (63%), Gaps = 10/217 (4%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRR-VSTLEILPFHRLSGQDGSIADE 445
           D++FANR KL+ GK FGCN+T LA AS+   W+HLR  VS  E     RL      +ADE
Sbjct: 90  DVVFANRPKLVPGKVFGCNFTNLAWASYDSHWKHLRSCVSCFEKQQSQRLPDL---LADE 146

Query: 444 VKLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEK---------KKAAEDDQL 292
           VKLL+  L     + ++LK +F +LVF+VMM+M+AGK + ++         +K  E   L
Sbjct: 147 VKLLIRQLCCNNNDAIQLKPMFQELVFNVMMRMYAGKSFSDRSCRYKTTGQQKENELISL 206

Query: 291 KDYAIRSFRITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPD 112
            +YAI SF++TT E+D+ YF PILK LG+ GLE++C +LQ++GD+ + +LI ++R+K   
Sbjct: 207 LEYAIHSFKMTTAESDLTYFRPILKLLGVTGLEQRCTQLQRKGDSLIHNLINKIREK--- 263

Query: 111 FSNGSGETKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
                   +E +I+FLL +QK+ P  Y+ + I GL+L
Sbjct: 264 -----SLKEESLIQFLLDKQKEDPLNYTKEIISGLVL 295


>ref|XP_023535659.1| isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo]
          Length = 498

 Score =  155 bits (393), Expect = 2e-41
 Identities = 89/210 (42%), Positives = 139/210 (66%), Gaps = 3/210 (1%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR  LI GK  G NYT+L  A +G  WR+LRRVS+LEI   HRL        DEV
Sbjct: 90  DIVFANRPHLIGGKYLGYNYTSLLWAPYGEHWRNLRRVSSLEIFSTHRLQTLSSIRVDEV 149

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKR-YGEKKKAAED-DQLKDYAIRSF 268
           + L+  L+ T+ ++V++++ F +L+F++MM+M AGKR YGE    +E+ ++ ++    +F
Sbjct: 150 RTLIRRLYNTENDVVDMRTEFFELMFNIMMRMIAGKRYYGEDAAESEEAERFREIQEETF 209

Query: 267 RITTKETDVGYFMPILKFLGL-RGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGE 91
           R+++K T++G F+ I+K+ GL +GLE +  +LQ + DA M+SLIEE RK+         +
Sbjct: 210 RLSSK-TNLGDFLRIVKWAGLSKGLENRMKELQSKRDAWMQSLIEEHRKQ------SKKD 262

Query: 90  TKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
            K+ +IE LL+ Q+  P+ Y D+ IRGL+L
Sbjct: 263 AKKTMIEVLLSLQQKEPRYYKDEYIRGLML 292


>ref|XP_022936848.1| isoflavone 2'-hydroxylase-like [Cucurbita moschata]
          Length = 498

 Score =  155 bits (393), Expect = 2e-41
 Identities = 89/210 (42%), Positives = 138/210 (65%), Gaps = 3/210 (1%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR  LI GK  G NYT+L  A +G  WR+LRR+S+LEI   HRL        DEV
Sbjct: 90  DIVFANRPHLIGGKYLGYNYTSLLWAPYGEHWRNLRRISSLEIFSTHRLQTLSSIRVDEV 149

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKR-YGEKKKAAED-DQLKDYAIRSF 268
           + L+  L+ TK ++V++++ F +L+F++MM+M AGKR YGE    +E+ ++ ++    +F
Sbjct: 150 RTLIRRLYNTKNDVVDMRTEFFELMFNIMMRMIAGKRYYGEDAAESEEAERFREIQEETF 209

Query: 267 RITTKETDVGYFMPILKFLGL-RGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGE 91
           R+++K T++G F+ I+K+ GL +GLE +  +LQ + DA M+SLIEE RK+         +
Sbjct: 210 RLSSK-TNLGDFLRIVKWAGLSKGLENRMKELQSKRDAWMQSLIEEHRKQ------SKKD 262

Query: 90  TKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
            K+ +IE LL+ Q+  P  Y D+ IRGL+L
Sbjct: 263 AKKTMIEVLLSLQQKEPHYYKDEYIRGLML 292


>ref|XP_022977008.1| isoflavone 2'-hydroxylase-like [Cucurbita maxima]
          Length = 498

 Score =  155 bits (392), Expect = 3e-41
 Identities = 89/210 (42%), Positives = 138/210 (65%), Gaps = 3/210 (1%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR  LI GK  G NYT+L  A +G  WR+LRR+S+LEI   HRL        DEV
Sbjct: 90  DIVFANRPHLIGGKYLGYNYTSLLWAPYGEHWRNLRRISSLEIFSTHRLQTLSSIRVDEV 149

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKR-YGEKKKAAED-DQLKDYAIRSF 268
           + L+  L+ TK ++V++K+ F +L+F++MM+M AGKR YGE    +E+ ++ ++    +F
Sbjct: 150 RTLIRRLYNTKNDVVDMKTEFFELMFNIMMRMIAGKRYYGEDVAESEEAERFREIQEETF 209

Query: 267 RITTKETDVGYFMPILKFLGL-RGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGE 91
           R+++K T++G F+ ++K+ GL +GLE +  +LQ + DA M+SLIEE RK+         +
Sbjct: 210 RLSSK-TNLGDFLRMVKWAGLSKGLENRMKELQSKRDAWMQSLIEEHRKQ------SKKD 262

Query: 90  TKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
            K+ +IE LL+ Q+  P  Y D+ IRGL+L
Sbjct: 263 AKKTMIEVLLSLQQKEPHYYKDEYIRGLML 292


>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
          Length = 499

 Score =  155 bits (392), Expect = 3e-41
 Identities = 90/209 (43%), Positives = 139/209 (66%), Gaps = 2/209 (0%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR +LI GK  G NYT+++ A HG  WR+LRR+S+ EIL  +RL    G  +DEV
Sbjct: 90  DVVFANRPRLIAGKHLGYNYTSMSWAPHGDHWRNLRRISSFEILSSNRLQTLSGIRSDEV 149

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKR-YGEKKKAAEDDQL-KDYAIRSF 268
           + L+  LF+ + ++VE+KS F ++  +VMMKM  GKR YGE     E+ ++ ++    +F
Sbjct: 150 RSLVRWLFKNQSQMVEMKSAFFEMTLNVMMKMIGGKRYYGENIGEVEEARMFREMVSETF 209

Query: 267 RITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGET 88
           ++    T++  F+PIL +LGL+G ER+  KLQK+ ++ +++LIEE R+K    SN  G  
Sbjct: 210 QL-AGATNMVDFLPILGWLGLKGTERRLIKLQKRRESFIQNLIEEHRRK---GSNCEGRQ 265

Query: 87  KEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
           K  +IE LL+ Q+  P+ Y+D+ IRGL+L
Sbjct: 266 K-TMIEVLLSLQETEPEYYTDEIIRGLML 293


>ref|XP_021691638.1| cytochrome P450 81E8-like [Hevea brasiliensis]
          Length = 329

 Score =  150 bits (380), Expect = 6e-41
 Identities = 88/208 (42%), Positives = 132/208 (63%), Gaps = 1/208 (0%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR +L+ GK  G NYT+LA A +G LWR+LR++S+LEIL  HRL       +DEV
Sbjct: 87  DVVFANRPRLLHGKHIGYNYTSLAWAPYGDLWRNLRKLSSLEILSSHRLQLLSSIRSDEV 146

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKR-YGEKKKAAEDDQLKDYAIRSFR 265
           KLL+  LF  K E ++LKS F +L+ +VMM+M AGKR YGE  +  E        +R   
Sbjct: 147 KLLIRRLFNNKDETLDLKSAFFELMLNVMMRMIAGKRYYGENVEEVEKAASFQKIVRETF 206

Query: 264 ITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGETK 85
           +    +++G F+P+L  +G  G+E++   LQK+ D  ++ LIEE RK++   ++ S E  
Sbjct: 207 LFAGTSNMGDFLPLLAKIG--GVEKRLLDLQKRRDGFIQVLIEEHRKRMS--TSPSEEKN 262

Query: 84  EKVIEFLLARQKDHPKRYSDKTIRGLLL 1
           + +IE LL  Q+  P  Y+D+TI+ L+L
Sbjct: 263 KTLIEVLLTLQQSDPDCYTDQTIKSLML 290


>ref|XP_008452618.1| PREDICTED: cytochrome P450 81E8-like [Cucumis melo]
          Length = 538

 Score =  155 bits (391), Expect = 7e-41
 Identities = 85/216 (39%), Positives = 143/216 (66%), Gaps = 9/216 (4%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR +L++GK  G NYT+L  A +G  WR+LRR+S+LEIL  HRL        DEV
Sbjct: 120 DVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEV 179

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAAEDDQ---LKDYAIRS 271
           + L+  L+  + ++V++++ F +L+F++MM+M AGKRY  +   A+ ++    ++    +
Sbjct: 180 RKLIRRLYNAENDVVDMRTEFFELMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEET 239

Query: 270 FRITTKETDVGYFMPILKFLGL-RGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGS- 97
           FR++ K T++G F+ ++K++GL +G+E +  +LQ + D  M+SLIEE RKK+ +  + S 
Sbjct: 240 FRLSGK-TNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSS 298

Query: 96  ----GETKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
               G+ K+ +IE LL+ Q+  P+ Y D+ IRGL+L
Sbjct: 299 SSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIRGLML 334


>ref|XP_021612036.1| cytochrome P450 81E8-like [Manihot esculenta]
 gb|OAY50994.1| hypothetical protein MANES_05G179100 [Manihot esculenta]
          Length = 495

 Score =  154 bits (388), Expect = 1e-40
 Identities = 90/209 (43%), Positives = 136/209 (65%), Gaps = 2/209 (0%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR +L+ GK  G N+T+LA A +G LWR+LR++S+LEIL  HRL        DEV
Sbjct: 87  DIVFANRPRLLAGKHIGYNFTSLAWAPYGDLWRNLRKLSSLEILSSHRLQLLSSIRNDEV 146

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKR-YGEKKKAAED-DQLKDYAIRSF 268
           KLL+  LFR K E+V+LKS F +L+ +VMM+M AGKR YGE  +  E  +  ++    +F
Sbjct: 147 KLLIHRLFRNKDEMVDLKSTFFELMLNVMMRMIAGKRYYGENVEEVEKAESFREIVRETF 206

Query: 267 RITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSGET 88
           ++    T +G F+P+L  +G  G+E++   LQK+ DA ++ LIEE R ++    + S E 
Sbjct: 207 QMA---TSMGDFLPLLAKIG--GIEKRLLDLQKRRDAFIQDLIEEHRSRM--MISPSEEK 259

Query: 87  KEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
            + +IE LL  Q+  P+ Y+D+TI+ L+L
Sbjct: 260 NKTLIEVLLTLQQSDPEYYTDQTIKNLML 288


>ref|XP_009354182.2| PREDICTED: cytochrome P450 81D11-like [Pyrus x bretschneideri]
          Length = 539

 Score =  152 bits (385), Expect = 5e-40
 Identities = 88/212 (41%), Positives = 137/212 (64%), Gaps = 6/212 (2%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D+ FANR  L+ GK  G NYT L  AS+G  WR++RR++++E+L  HRL    G   +EV
Sbjct: 124 DVAFANRPGLLAGKHLGYNYTTLVWASYGTHWRNMRRIASIELLSSHRLQMFYGIRVEEV 183

Query: 441 KLLLGGLFRTKK----EIVELKSVFLDLVFDVMMKMFAGKR-YGEKKKAAEDDQL-KDYA 280
           + LL  LFR  K    +I+++KS F +L  +V+M+M AGKR YGE+ + +E+ QL K+  
Sbjct: 184 RSLLSRLFRGSKPGEFQILDMKSTFFELTLNVLMRMIAGKRYYGEQTEKSEEAQLFKEMV 243

Query: 279 IRSFRITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNG 100
           I +F + +  T++G FMP+LK LG+ GLE+K   LQK+ D  M++LIEE RK      + 
Sbjct: 244 IETFEL-SGATNIGDFMPVLKHLGVTGLEKKLVLLQKKKDEFMQNLIEEYRKL--QRGSV 300

Query: 99  SGETKEKVIEFLLARQKDHPKRYSDKTIRGLL 4
           S +  + +++ LL  Q+  P+ YS++ +RG++
Sbjct: 301 SEQRSKSMVDVLLDLQETDPEYYSNEIVRGMI 332


>gb|OAY50996.1| hypothetical protein MANES_05G179300 [Manihot esculenta]
          Length = 496

 Score =  152 bits (383), Expect = 6e-40
 Identities = 89/213 (41%), Positives = 136/213 (63%), Gaps = 6/213 (2%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR +L+ GK  G N+T+LA A +G LWR+ R++S+LEIL  HRL    G  +DEV
Sbjct: 87  DIIFANRPRLLAGKHIGYNFTSLAWAPYGDLWRNHRKLSSLEILSSHRLELLSGIRSDEV 146

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEK--KKAAEDDQLKDYAIRSF 268
           KLL+  LFR K EIV+LKS F  L+ +VMM+M AGKRY  +  ++  E    ++    +F
Sbjct: 147 KLLIRRLFRNKDEIVDLKSAFFQLILNVMMRMIAGKRYYNENVEEVEEVASFQEIVRETF 206

Query: 267 RITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKK----IPDFSNG 100
           ++ T  +++G F+P+L  +G  G+E++   LQK+ D  ++SLIEE R K    +PD    
Sbjct: 207 QMAT--SNMGDFLPLLAKVG--GVEKRLLDLQKRRDGFIQSLIEEHRNKMTISLPD---- 258

Query: 99  SGETKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
             E  +  IE LL  Q+  P+ Y+D+TI+ +++
Sbjct: 259 --EKNKTFIEVLLTLQQSDPESYTDQTIKSIMM 289


>ref|XP_021612537.1| cytochrome P450 81D1-like [Manihot esculenta]
          Length = 506

 Score =  152 bits (383), Expect = 7e-40
 Identities = 89/213 (41%), Positives = 136/213 (63%), Gaps = 6/213 (2%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR +L+ GK  G N+T+LA A +G LWR+ R++S+LEIL  HRL    G  +DEV
Sbjct: 97  DIIFANRPRLLAGKHIGYNFTSLAWAPYGDLWRNHRKLSSLEILSSHRLELLSGIRSDEV 156

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEK--KKAAEDDQLKDYAIRSF 268
           KLL+  LFR K EIV+LKS F  L+ +VMM+M AGKRY  +  ++  E    ++    +F
Sbjct: 157 KLLIRRLFRNKDEIVDLKSAFFQLILNVMMRMIAGKRYYNENVEEVEEVASFQEIVRETF 216

Query: 267 RITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKK----IPDFSNG 100
           ++ T  +++G F+P+L  +G  G+E++   LQK+ D  ++SLIEE R K    +PD    
Sbjct: 217 QMAT--SNMGDFLPLLAKVG--GVEKRLLDLQKRRDGFIQSLIEEHRNKMTISLPD---- 268

Query: 99  SGETKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
             E  +  IE LL  Q+  P+ Y+D+TI+ +++
Sbjct: 269 --EKNKTFIEVLLTLQQSDPESYTDQTIKSIMM 299


>ref|XP_008378166.1| PREDICTED: isoflavone 2'-hydroxylase-like [Malus domestica]
          Length = 506

 Score =  151 bits (382), Expect = 9e-40
 Identities = 90/214 (42%), Positives = 136/214 (63%), Gaps = 8/214 (3%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D+ FANR  L+ GK  G NYT L  AS+G  WR++RR++++E+L  HRL    G   +EV
Sbjct: 91  DVAFANRPGLLAGKHLGYNYTTLVWASYGTHWRNMRRIASIELLSSHRLQMFYGIRVEEV 150

Query: 441 KLLLGGLFRTKK----EIVELKSVFLDLVFDVMMKMFAGKR-YGEKKKAAEDDQL-KDYA 280
           + LL  LFR  K    +I+++KS F +L  +V+M+M AGKR YGE+ +  E+ QL K+  
Sbjct: 151 RSLLSRLFRGSKPGEFQILDMKSTFFELTLNVLMRMIAGKRYYGEQTEKLEEAQLFKEIV 210

Query: 279 IRSFRITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNG 100
           I +F + +  T++G FMP+LK LG+ GLE+K   LQK+ D  M++LIEE RK       G
Sbjct: 211 IETFEL-SGATNIGDFMPVLKHLGVTGLEKKLVLLQKKRDKFMQNLIEEHRK----LQRG 265

Query: 99  S--GETKEKVIEFLLARQKDHPKRYSDKTIRGLL 4
           S   +  + +++ LL  ++  P+ YSD+ IRG++
Sbjct: 266 SVPEQRSKSMVDVLLDLKETEPEYYSDEIIRGMI 299


>ref|XP_010654768.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 512

 Score =  151 bits (382), Expect = 1e-39
 Identities = 89/219 (40%), Positives = 141/219 (64%), Gaps = 12/219 (5%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR +LI GK  G NYT+LA A++G  WR+LRR+S+LEIL   R+    G  ADEV
Sbjct: 90  DIVFANRPQLIAGKYIGYNYTSLAWANYGDHWRNLRRISSLEILSSSRIQMLSGIRADEV 149

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRY--GEKKKAAEDDQLKDYAIRSF 268
           +LL+  L   + + V +K++  ++  +VMM+M AGKRY  G   +A E  + ++    + 
Sbjct: 150 RLLVRWLLENENQTVNVKAMLFEITTNVMMRMIAGKRYYGGSMAEAEETVKFREIIADTL 209

Query: 267 RITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPD-------F 109
           R+    T+VG ++P+L++LG++G+E+   +LQ++ D  M+SLIEE R ++ +        
Sbjct: 210 RL-GDTTNVGDYLPMLRWLGVKGMEKGLRELQRKRDRFMQSLIEEHRTRMAEEKESYSSC 268

Query: 108 SNG-SGETKEK--VIEFLLARQKDHPKRYSDKTIRGLLL 1
           SNG  GE K+K  +IE +L+ Q+  P  Y+D+ IRGL+L
Sbjct: 269 SNGDDGEKKKKKTMIEVMLSLQEKEPDYYTDQIIRGLML 307


>ref|XP_024162938.1| cytochrome P450 81D11-like [Rosa chinensis]
 gb|PRQ27788.1| putative oxidoreductase [Rosa chinensis]
          Length = 506

 Score =  150 bits (379), Expect = 2e-39
 Identities = 85/211 (40%), Positives = 132/211 (62%), Gaps = 5/211 (2%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D+ FANR KL+ GK FGCNYTA+  AS+GP WR+LRR+++LE+L   RL    G  ADEV
Sbjct: 91  DVTFANRPKLLAGKHFGCNYTAIVWASYGPHWRNLRRIASLELLSSSRLQMFHGIRADEV 150

Query: 441 KLLLGGLFR----TKKEIVELKSVFLDLVFDVMMKMFAGKR-YGEKKKAAEDDQLKDYAI 277
           + L+  LFR     +   +++KS F +L  +V+M++ AGKR YGE    + + +  +  +
Sbjct: 151 RSLICKLFRGYNGGELRSLDMKSTFFELTLNVLMRVIAGKRYYGEDIAGSSEAKSFEQIV 210

Query: 276 RSFRITTKETDVGYFMPILKFLGLRGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGS 97
           +     +   ++G F+P+LK+LG  GLE+K  KLQ++ D  M+ LIEE RK      + S
Sbjct: 211 KETFQLSGANNIGDFLPVLKYLGSGGLEKKMLKLQEKRDKFMQYLIEEHRK--VQSGSVS 268

Query: 96  GETKEKVIEFLLARQKDHPKRYSDKTIRGLL 4
            +  + +++ LL+ Q   P+ Y+DK IRG++
Sbjct: 269 EQKSQTMVDVLLSLQATEPEYYTDKIIRGIM 299


>ref|XP_004141396.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis sativus]
 ref|XP_011654142.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis sativus]
 gb|KGN55248.1| hypothetical protein Csa_4G642290 [Cucumis sativus]
          Length = 507

 Score =  150 bits (378), Expect = 4e-39
 Identities = 84/215 (39%), Positives = 141/215 (65%), Gaps = 8/215 (3%)
 Frame = -2

Query: 621 DLLFANRSKLITGKEFGCNYTALASASHGPLWRHLRRVSTLEILPFHRLSGQDGSIADEV 442
           D++FANR +L++GK  G NYT+L  A +G  WR+LRR+S+LEIL  HRL        DEV
Sbjct: 90  DVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEV 149

Query: 441 KLLLGGLFRTKKEIVELKSVFLDLVFDVMMKMFAGKRYGEKKKAA---EDDQLKDYAIRS 271
           + L+  L+  + ++V++++ F +++F++ M+M AGKRY  +  AA   E  + ++    +
Sbjct: 150 RTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGKRYYGEDVAAYSEEAGRFREIQEET 209

Query: 270 FRITTKETDVGYFMPILKFLGL-RGLERKCNKLQKQGDAAMESLIEEVRKKIPDFSNGSG 94
           FR++ K T++G F+ ++K++GL +G+E +  +LQ + D  M+SLIEE RKK+ +  + S 
Sbjct: 210 FRLSGK-TNLGDFLRVVKWVGLSKGIENRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSS 268

Query: 93  ----ETKEKVIEFLLARQKDHPKRYSDKTIRGLLL 1
               + K+ +IE LL+ Q+  P+ Y D+ IRGL+L
Sbjct: 269 SIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLML 303