BLASTX nr result
ID: Chrysanthemum22_contig00011828
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Chrysanthemum22_contig00011828
(681 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|OTF93917.1| putative debranching enzyme 1 [Helianthus annuus] 251 1e-74
ref|XP_022010633.1| isoamylase 2, chloroplastic [Helianthus annu... 236 2e-69
ref|XP_023738402.1| isoamylase 2, chloroplastic isoform X1 [Lact... 232 1e-67
gb|KVH94413.1| Glycoside hydrolase, catalytic domain-containing ... 225 5e-65
ref|XP_023738403.1| isoamylase 2, chloroplastic isoform X2 [Lact... 207 2e-58
gb|AOQ26242.1| ISA2 [Actinidia deliciosa] 130 5e-31
ref|XP_021613460.1| isoamylase 2, chloroplastic [Manihot esculen... 112 9e-25
ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nico... 110 3e-24
ref|XP_009356744.1| PREDICTED: isoamylase 2, chloroplastic-like ... 108 1e-23
ref|XP_008386532.1| PREDICTED: isoamylase 2, chloroplastic-like ... 108 1e-23
ref|XP_018858427.1| PREDICTED: isoamylase 2, chloroplastic [Jugl... 108 3e-23
ref|XP_021681351.1| isoamylase 2, chloroplastic isoform X2 [Heve... 106 9e-23
ref|XP_021681350.1| isoamylase 2, chloroplastic isoform X1 [Heve... 106 9e-23
gb|PON52856.1| Glycoside hydrolase [Trema orientalis] 106 1e-22
gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum] 105 2e-22
ref|XP_008368864.1| PREDICTED: isoamylase 2, chloroplastic-like ... 105 3e-22
gb|PON65768.1| Glycoside hydrolase [Parasponia andersonii] 104 4e-22
ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea... 102 3e-21
ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nico... 102 3e-21
ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber] ... 101 5e-21
>gb|OTF93917.1| putative debranching enzyme 1 [Helianthus annuus]
Length = 847
Score = 251 bits (640), Expect = 1e-74
Identities = 127/191 (66%), Positives = 151/191 (79%), Gaps = 3/191 (1%)
Frame = +2
Query: 116 FWFLQRICLHSWLDITMAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKN 295
FWFLQ ICL S L M+ML+ SSATQP CLTCGTIK+S LI +D+CKYG R TQ+FG+
Sbjct: 4 FWFLQVICLQSAL---MSMLSLSSATQPTCLTCGTIKISKLIIADSCKYGRRATQSFGRM 60
Query: 296 NLGRNLVLNEVA---IKGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVIT 466
+LG+NLV + A IKG Y R +LA ST+PV QT +KLSTY FRTEKGGNV V+IT
Sbjct: 61 DLGQNLVFGKTAKNIIKGLYHNPRFGILAASTLPVIQTAEKLSTYLFRTEKGGNVTVIIT 120
Query: 467 KLKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLSDSGIFETPFVNNLLN 646
+LK+GKYGVN+E SSLQL+GND D++MSWG+FRSDSSS +P+ SDSGIFETPF + LN
Sbjct: 121 RLKDGKYGVNVEISSLQLTGNDADIIMSWGVFRSDSSSRIPIHSSDSGIFETPFAIDSLN 180
Query: 647 KLSVELEFDAS 679
K S ELEFDAS
Sbjct: 181 KFSAELEFDAS 191
>ref|XP_022010633.1| isoamylase 2, chloroplastic [Helianthus annuus]
ref|XP_022010634.1| isoamylase 2, chloroplastic [Helianthus annuus]
Length = 831
Score = 236 bits (603), Expect = 2e-69
Identities = 117/175 (66%), Positives = 141/175 (80%), Gaps = 3/175 (1%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---I 334
M+ML+ SSATQP CLTCGTIK+S LI +D+CKYG R TQ+FG+ +LG+NLV + A I
Sbjct: 1 MSMLSLSSATQPTCLTCGTIKISKLIIADSCKYGRRATQSFGRMDLGQNLVFGKTAKNII 60
Query: 335 KGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSL 514
KG Y R +LA ST+PV QT +KLSTY FRTEKGGNV V+IT+LK+GKYGVN+E SSL
Sbjct: 61 KGLYHNPRFGILAASTLPVIQTAEKLSTYLFRTEKGGNVTVIITRLKDGKYGVNVEISSL 120
Query: 515 QLSGNDDDVVMSWGIFRSDSSSLMPLQLSDSGIFETPFVNNLLNKLSVELEFDAS 679
QL+GND D++MSWG+FRSDSSS +P+ SDSGIFETPF + LNK S ELEFDAS
Sbjct: 121 QLTGNDADIIMSWGVFRSDSSSRIPIHSSDSGIFETPFAIDSLNKFSAELEFDAS 175
>ref|XP_023738402.1| isoamylase 2, chloroplastic isoform X1 [Lactuca sativa]
Length = 876
Score = 232 bits (592), Expect = 1e-67
Identities = 129/196 (65%), Positives = 146/196 (74%), Gaps = 7/196 (3%)
Frame = +2
Query: 113 IFWFLQRICLHSWL----DITMAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQ 280
I WFLQ +CLHSW+ DI+ AM++ SSATQP LTCGTIK+S LI +D+CKYG RV Q
Sbjct: 27 IIWFLQVLCLHSWVVLHSDISTAMISLSSATQPSYLTCGTIKVSKLIIADSCKYGRRVAQ 86
Query: 281 NFGKNNLGRNLVLNEVA---IKGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNV 451
G+ N+ +NLV VA IK S RRI VLA ST PV Q KLSTY FRTEKGG+V
Sbjct: 87 TPGRINVEQNLVFGLVAKNMIKDSCHARRISVLAASTFPVIQPANKLSTYLFRTEKGGHV 146
Query: 452 KVVITKLKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLSDSGIFETPFV 631
KV IT+LKN KY VN+E S L NDDDV+MSWGIFRSDSSSL+PL+ SDSGI ETPFV
Sbjct: 147 KV-ITELKNDKYRVNLEVSPLH--DNDDDVIMSWGIFRSDSSSLIPLESSDSGISETPFV 203
Query: 632 NNLLNKLSVELEFDAS 679
N LNKLS ELEFDAS
Sbjct: 204 NKSLNKLSTELEFDAS 219
>gb|KVH94413.1| Glycoside hydrolase, catalytic domain-containing protein [Cynara
cardunculus var. scolymus]
Length = 841
Score = 225 bits (573), Expect = 5e-65
Identities = 121/175 (69%), Positives = 141/175 (80%), Gaps = 3/175 (1%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---I 334
MAML+ SSAT P LTCGT KLS LI +D+CKYG RVT++ G+ ++ R+LV EVA I
Sbjct: 1 MAMLSLSSATHPRYLTCGTTKLSKLIIADSCKYGRRVTRSLGRMDMERSLVFGEVAKNMI 60
Query: 335 KGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSL 514
KGS R+ VLA ST+PV +TV+KLSTY FRTEKGG+VKV IT+LK+ KYGV++E S L
Sbjct: 61 KGSCHNLRVGVLAASTIPVIETVEKLSTYLFRTEKGGHVKV-ITRLKDDKYGVDVEVSYL 119
Query: 515 QLSGNDDDVVMSWGIFRSDSSSLMPLQLSDSGIFETPFVNNLLNKLSVELEFDAS 679
QLSGND DVVMSWGIFRSDSSSLM L+ +DSGI ETPFVN LNKLSVELEFDAS
Sbjct: 120 QLSGNDGDVVMSWGIFRSDSSSLMSLRSNDSGILETPFVNKSLNKLSVELEFDAS 174
>ref|XP_023738403.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
ref|XP_023738404.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
ref|XP_023738405.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
ref|XP_023738406.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
gb|PLY70241.1| hypothetical protein LSAT_9X4440 [Lactuca sativa]
Length = 827
Score = 207 bits (527), Expect = 2e-58
Identities = 116/173 (67%), Positives = 130/173 (75%), Gaps = 3/173 (1%)
Frame = +2
Query: 170 MLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---IKG 340
M++ SSATQP LTCGTIK+S LI +D+CKYG RV Q G+ N+ +NLV VA IK
Sbjct: 1 MISLSSATQPSYLTCGTIKVSKLIIADSCKYGRRVAQTPGRINVEQNLVFGLVAKNMIKD 60
Query: 341 SYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSLQL 520
S RRI VLA ST PV Q KLSTY FRTEKGG+VKV IT+LKN KY VN+E S L
Sbjct: 61 SCHARRISVLAASTFPVIQPANKLSTYLFRTEKGGHVKV-ITELKNDKYRVNLEVSPLH- 118
Query: 521 SGNDDDVVMSWGIFRSDSSSLMPLQLSDSGIFETPFVNNLLNKLSVELEFDAS 679
NDDDV+MSWGIFRSDSSSL+PL+ SDSGI ETPFVN LNKLS ELEFDAS
Sbjct: 119 -DNDDDVIMSWGIFRSDSSSLIPLESSDSGISETPFVNKSLNKLSTELEFDAS 170
>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
Length = 866
Score = 130 bits (327), Expect = 5e-31
Identities = 81/181 (44%), Positives = 105/181 (58%), Gaps = 9/181 (4%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEV---AI 334
MA L S Q C+ CG + S + + Y RV QN + + RN V EV A
Sbjct: 1 MATLPLSFTIQSHCVYCGATESSKSVVATHFNYRKRVAQNL-QQSYKRNFVHGEVKKYAT 59
Query: 335 KGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSL 514
K S++ R ++VLATS + V Q +K S Y FRTE GG+VKV++ + KN KY V +E SSL
Sbjct: 60 KDSFQHRNLKVLATSRISVGQKEEKSSAYLFRTEIGGHVKVLVRQ-KNLKYSVYVEVSSL 118
Query: 515 QLSGNDDDVVMSWGIFRSDSSSLMPLQLSD------SGIFETPFVNNLLNKLSVELEFDA 676
+L +D +VM+WGIFRSDSS +PL L S ETPFV N L KL +EL F+
Sbjct: 119 KLKSGEDKLVMNWGIFRSDSSQFIPLDLQSSTPDDRSSTVETPFVQNSLGKLVIELNFEG 178
Query: 677 S 679
S
Sbjct: 179 S 179
>ref|XP_021613460.1| isoamylase 2, chloroplastic [Manihot esculenta]
gb|OAY49666.1| hypothetical protein MANES_05G073400 [Manihot esculenta]
gb|AUZ20773.1| isoamylase 2 (plastid) [Manihot esculenta]
Length = 883
Score = 112 bits (280), Expect = 9e-25
Identities = 72/190 (37%), Positives = 102/190 (53%), Gaps = 19/190 (10%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAIK-- 337
MA L S A C +CG ++ S L + G ++ FG+ ++ + L++ EVA
Sbjct: 1 MATLLPSFAISRCCYSCGAVESSKLTLTTRYTSGKKMELGFGRTDVEKRLLVGEVAQNVR 60
Query: 338 ---------GSYRTRRIQVLAT----STVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKN 478
G + R+ V T ST+ ++K+S+Y FRT+ GGNVKV + K KN
Sbjct: 61 STLHWNHNSGVFAAARVPVQETEQILSTITEVDELQKVSSYLFRTQIGGNVKVSVRK-KN 119
Query: 479 GKYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLSD----SGIFETPFVNNLLN 646
KY V IE SSL+L +D +V++WGI+RSDSS MPL + ETPFV N
Sbjct: 120 AKYAVYIEVSSLELGNSDYRLVLAWGIYRSDSSCFMPLDSQRLDPVARTMETPFVQNAFA 179
Query: 647 KLSVELEFDA 676
S+ELEF+A
Sbjct: 180 IFSLELEFEA 189
>ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana attenuata]
gb|OIT28559.1| isoamylase 2, chloroplastic [Nicotiana attenuata]
Length = 863
Score = 110 bits (276), Expect = 3e-24
Identities = 74/187 (39%), Positives = 101/187 (54%), Gaps = 15/187 (8%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAIKG- 340
MA LT A Q L+ G + + L+ + +G RV R L L E+ G
Sbjct: 1 MATLTVQLAVQSRLLSYGIGESTKLVPAPPANHGKRVLCRL------RKLELEEINFSGR 54
Query: 341 --------SYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVN 496
S RT R++ L+TS + + QT K + TY FRT+ GG VKV++ K NGKY V
Sbjct: 55 SRKTDQEVSKRTHRLKALSTSGISLIQTAKTVPTYLFRTDTGGQVKVLVEKT-NGKYKVL 113
Query: 497 IEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLS------DSGIFETPFVNNLLNKLSV 658
+EA +LS ++VM WG+FRSD+S MPL L+ S ETPFV NK++V
Sbjct: 114 VEALPSELSDAHAELVMVWGLFRSDASCFMPLDLNRRGADGKSSTIETPFVQGPSNKVTV 173
Query: 659 ELEFDAS 679
EL+F+AS
Sbjct: 174 ELDFEAS 180
>ref|XP_009356744.1| PREDICTED: isoamylase 2, chloroplastic-like [Pyrus x
bretschneideri]
Length = 870
Score = 108 bits (271), Expect = 1e-23
Identities = 82/196 (41%), Positives = 104/196 (53%), Gaps = 25/196 (12%)
Frame = +2
Query: 164 MAMLTSSSATQPP-CLTCGTIKLSTLIFSDTCKY---GTRVTQNFGKNNLGRNLVLNEVA 331
MA L S A Q CL CGT +LS L ++ + G RV+ G + RNLV EV
Sbjct: 1 MATLPLSIAIQASFCLNCGTTELSKLTAANRSIHRHNGLRVSVKLG---IERNLVFGEVL 57
Query: 332 I---KGSYRTRRIQVLATSTVPV------------TQTVKKLSTYSFRTEKGGNVKVVIT 466
+ R R ++V ATS V V T+ K+STY FRTE G VKV +
Sbjct: 58 QNFKETPLRDRDLKVYATSRVSVEPMEQKVYTSTETEEAGKVSTYRFRTETGDLVKVFV- 116
Query: 467 KLKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLSDS------GIFETPF 628
++KN KY VNIE SSL LS ND +V+SWGI+RSD+SS MP S ETPF
Sbjct: 117 RMKNAKYIVNIEVSSLHLSSNDRLLVLSWGIYRSDASSFMPSNFRSSTPADRTTTLETPF 176
Query: 629 VNNLLNKLSVELEFDA 676
+ ++ELEF+A
Sbjct: 177 TETSSGRFTLELEFEA 192
>ref|XP_008386532.1| PREDICTED: isoamylase 2, chloroplastic-like [Malus domestica]
Length = 870
Score = 108 bits (271), Expect = 1e-23
Identities = 79/193 (40%), Positives = 101/193 (52%), Gaps = 22/193 (11%)
Frame = +2
Query: 164 MAMLTSSSATQPPC-LTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAI-- 334
MA L S A Q C L CGT +LS L ++ ++ + K + RNLV EV
Sbjct: 1 MATLPLSIAMQASCCLNCGTTELSKLTAANRYRHRHNGLRGSVKLGIERNLVFGEVVQNF 60
Query: 335 -KGSYRTRRIQVLATSTVPV------------TQTVKKLSTYSFRTEKGGNVKVVITKLK 475
+ R R ++V ATS V V T+ K+STY FRTE G VKV + ++K
Sbjct: 61 KETPLRDRDLKVYATSRVSVEPMEQRVYTSTETEEAGKVSTYRFRTETGDMVKVFV-RMK 119
Query: 476 NGKYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLSDS------GIFETPFVNN 637
N K VNIE SSL LS ND +V+SWGI+RSDSSS MP S ETPF
Sbjct: 120 NAKCIVNIEVSSLHLSSNDRLLVLSWGIYRSDSSSFMPSNFRSSTPADRTTTLETPFTET 179
Query: 638 LLNKLSVELEFDA 676
+ ++ELEF+A
Sbjct: 180 CSGRFTLELEFEA 192
>ref|XP_018858427.1| PREDICTED: isoamylase 2, chloroplastic [Juglans regia]
Length = 889
Score = 108 bits (269), Expect = 3e-23
Identities = 73/188 (38%), Positives = 100/188 (53%), Gaps = 18/188 (9%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAIKGS 343
MA L+ S A +P CLTCG + S + Y ++ +L R L+ EVA K S
Sbjct: 1 MATLSPSLAIRPCCLTCGASQSSKSSLAYHYIYRNQIRCGIENTDLERKLICGEVA-KTS 59
Query: 344 YRTRRIQVLATSTVPVTQTVKKLST------------YSFRTEKGGNVKVVITKLKNGKY 487
YR +V ATS V + +T ++++T Y F TE GG VKV + K KN KY
Sbjct: 60 YRHLNSKVYATSRVFIKETEQRVTTITEVEDMLKSLTYLFWTEIGGQVKVSVRK-KNVKY 118
Query: 488 GVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLSDS------GIFETPFVNNLLNK 649
V +E SSLQL G+DD +++SWGI+R DSS M L S ETPF+ N +
Sbjct: 119 AVYVEVSSLQLHGSDDRLLLSWGIYRDDSSCFMHLDAQSSTPDGRATTRETPFIQNTKGR 178
Query: 650 LSVELEFD 673
+EL+F+
Sbjct: 179 FVLELDFE 186
>ref|XP_021681351.1| isoamylase 2, chloroplastic isoform X2 [Hevea brasiliensis]
Length = 842
Score = 106 bits (265), Expect = 9e-23
Identities = 74/192 (38%), Positives = 104/192 (54%), Gaps = 22/192 (11%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---- 331
MA L S A P +C ++ S L + G ++ G ++ + L++ EVA
Sbjct: 1 MATLPLSLAKSPCFYSCRAVESSKLTVTAHYISGKKLALGLGGMDVEKGLLVGEVAQNVR 60
Query: 332 --IKGSYRTRRIQVLATSTVPVTQT------------VKKLSTYSFRTEKGGNVKVVITK 469
+ G+ +R A + VPV QT +KK+STY FRT+ GGNVKV + K
Sbjct: 61 KALHGNLSSREF---AAARVPVQQTEQIFSTTSEVDELKKVSTYLFRTQNGGNVKVFVRK 117
Query: 470 LKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPL--QLSDSG--IFETPFVNN 637
KN KY + IE SSL+L +D +V++WGI+RSDSS MPL Q D G +TPFV N
Sbjct: 118 -KNAKYALYIEVSSLELGTSDYRLVLTWGIYRSDSSCFMPLDSQHLDPGARTMDTPFVQN 176
Query: 638 LLNKLSVELEFD 673
+ S+ELEF+
Sbjct: 177 TFGRFSLELEFE 188
>ref|XP_021681350.1| isoamylase 2, chloroplastic isoform X1 [Hevea brasiliensis]
Length = 883
Score = 106 bits (265), Expect = 9e-23
Identities = 74/192 (38%), Positives = 104/192 (54%), Gaps = 22/192 (11%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---- 331
MA L S A P +C ++ S L + G ++ G ++ + L++ EVA
Sbjct: 1 MATLPLSLAKSPCFYSCRAVESSKLTVTAHYISGKKLALGLGGMDVEKGLLVGEVAQNVR 60
Query: 332 --IKGSYRTRRIQVLATSTVPVTQT------------VKKLSTYSFRTEKGGNVKVVITK 469
+ G+ +R A + VPV QT +KK+STY FRT+ GGNVKV + K
Sbjct: 61 KALHGNLSSREF---AAARVPVQQTEQIFSTTSEVDELKKVSTYLFRTQNGGNVKVFVRK 117
Query: 470 LKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPL--QLSDSG--IFETPFVNN 637
KN KY + IE SSL+L +D +V++WGI+RSDSS MPL Q D G +TPFV N
Sbjct: 118 -KNAKYALYIEVSSLELGTSDYRLVLTWGIYRSDSSCFMPLDSQHLDPGARTMDTPFVQN 176
Query: 638 LLNKLSVELEFD 673
+ S+ELEF+
Sbjct: 177 TFGRFSLELEFE 188
>gb|PON52856.1| Glycoside hydrolase [Trema orientalis]
Length = 880
Score = 106 bits (264), Expect = 1e-22
Identities = 69/189 (36%), Positives = 99/189 (52%), Gaps = 18/189 (9%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---I 334
MA L S AT C+ CG K S L ++ +V K NL R L+ ++
Sbjct: 1 MATLPPSLATSCCCVNCGATKSSKLKLANRILCRNKVFNGLDKMNLERKLLFGQIVPHFS 60
Query: 335 KGSYRTRRIQVLATSTVPV-----------TQTVKKLSTYSFRTEKGGNVKVVITKLKNG 481
S R ++V ATS V + T+ + K+S+Y FRTE G VKV++ K K+
Sbjct: 61 NTSLRNTNMEVNATSRVSIPVKQRFSRKTETEELDKVSSYLFRTEVGDLVKVLVKK-KSV 119
Query: 482 KYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLS----DSGIFETPFVNNLLNK 649
GV+IE SSL L +DD +++ WGI+R DSS+ MP++ S D ETPF+ K
Sbjct: 120 NCGVHIEVSSLPLFNSDDSLILKWGIYRGDSSNFMPIEFSTSAPDDRTVETPFLKTSFGK 179
Query: 650 LSVELEFDA 676
VE+EF++
Sbjct: 180 FVVEMEFES 188
>gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]
Length = 844
Score = 105 bits (263), Expect = 2e-22
Identities = 67/168 (39%), Positives = 99/168 (58%), Gaps = 9/168 (5%)
Frame = +2
Query: 203 CLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRN---LVLNEVAIKGSYRTRRIQVLA 373
C +CG + S L + KY VT++ K +L + + LN+ + S ++ LA
Sbjct: 8 CPSCGASESSYLFAAKFGKYVEWVTRDLVKFDLKQKKDRVRLNKFGGRISLEHHVMKALA 67
Query: 374 TSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSLQLSGNDDDVVMSW 553
TS V V QT KL++Y FRTE GG +KV++ KN K+ V++EAS+L D+ ++M W
Sbjct: 68 TSDVSVVQTTGKLASYRFRTEIGGQLKVLVR--KNSKFEVHVEASNLP-GEEDEGLIMRW 124
Query: 554 GIFRSDSSSLMPLQLSDS------GIFETPFVNNLLNKLSVELEFDAS 679
G+FRSDSS MPL S + ETPF+ + + +L+VEL+FDAS
Sbjct: 125 GLFRSDSSIFMPLNFQQSTADSKDNVVETPFLKDSVGRLAVELDFDAS 172
>ref|XP_008368864.1| PREDICTED: isoamylase 2, chloroplastic-like [Malus domestica]
Length = 870
Score = 105 bits (261), Expect = 3e-22
Identities = 81/195 (41%), Positives = 101/195 (51%), Gaps = 24/195 (12%)
Frame = +2
Query: 164 MAMLTSSSATQPPC-LTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLG--RNLVLNEVAI 334
MA L S A Q C L CGT +L L +D +YG R G LG RNLVL EV
Sbjct: 1 MATLPLSIAIQASCCLNCGTTELPKLTATD--RYGHRNNGVHGYVKLGTERNLVLREVVQ 58
Query: 335 ---KGSYRTRRIQVLATSTVPV------------TQTVKKLSTYSFRTEKGGNVKVVITK 469
+ R R +V ATS V V T+ V K+STY FRT+ G V V + K
Sbjct: 59 ILKERPLRDREWKVYATSRVSVEPVEQRVSTGTETEEVGKVSTYLFRTDTGDPVNVFVRK 118
Query: 470 LKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLSDS------GIFETPFV 631
+N KY VNIE S L LS +D +V+SWG++R+DSSS +P S ETPF
Sbjct: 119 -RNAKYAVNIEVSLLHLSRSDRRLVLSWGMYRADSSSFVPSNFRSSTPADRTTALETPFT 177
Query: 632 NNLLNKLSVELEFDA 676
K ++ELEF+A
Sbjct: 178 GTSSGKFTLELEFEA 192
>gb|PON65768.1| Glycoside hydrolase [Parasponia andersonii]
Length = 880
Score = 104 bits (260), Expect = 4e-22
Identities = 69/189 (36%), Positives = 98/189 (51%), Gaps = 18/189 (9%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAIKGS 343
MA L S A C+ CG K S L ++ +V K NL R L+ ++ S
Sbjct: 1 MATLPPSLALSCCCVNCGATKSSKLKLANRILCRNKVFNRLDKMNLERKLLFGQIVPHFS 60
Query: 344 ---YRTRRIQVLATSTVPV-----------TQTVKKLSTYSFRTEKGGNVKVVITKLKNG 481
R ++V ATS V + + K+S+Y FRTE GG VKV++ K K+
Sbjct: 61 NTFLRNTNMEVNATSRVSIPVKERFSRKTEAEEPDKVSSYLFRTEVGGLVKVLVKK-KSV 119
Query: 482 KYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLS----DSGIFETPFVNNLLNK 649
GV+IE SSL LS +DD +++ WGI+R DSS+ MP++ S D ETPF+ K
Sbjct: 120 NCGVHIEVSSLPLSNSDDSLILKWGIYRGDSSNFMPIEFSTSAPDDRTVETPFLKTSFGK 179
Query: 650 LSVELEFDA 676
VE+EF++
Sbjct: 180 FVVEMEFES 188
>ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea europaea var.
sylvestris]
Length = 849
Score = 102 bits (254), Expect = 3e-21
Identities = 68/171 (39%), Positives = 95/171 (55%), Gaps = 9/171 (5%)
Frame = +2
Query: 194 QPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAI---KGSYRTRRIQ 364
Q CL+ G ++LS S CKY VT F K + VL+ + + S+ +
Sbjct: 6 QSQCLSLGALELSKFSAS-CCKYDKGVTCGFQKMDAKYKQVLSGLGKSVRRISHELHTWK 64
Query: 365 VLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSLQLSGNDDDVV 544
LATS V V QT +K++ Y FRTE GG +K+++ K N KYGV +E S SG D++
Sbjct: 65 ALATSDVSV-QTAEKVAIYRFRTENGGQLKLLVRK-NNKKYGVYVEVLSPLHSGRKGDLL 122
Query: 545 MSWGIFRSDSSSLMPLQLSDS------GIFETPFVNNLLNKLSVELEFDAS 679
MSWG+FRS+S S MP+ +S +TPFV + +L VEL+FD S
Sbjct: 123 MSWGLFRSNSESFMPMDFENSSADSKYSTIDTPFVKDSSGRLVVELDFDES 173
>ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
ref|XP_018625653.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
Length = 863
Score = 102 bits (253), Expect = 3e-21
Identities = 69/184 (37%), Positives = 100/184 (54%), Gaps = 12/184 (6%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNL------GRNLVLNE 325
MA T A Q L+ G + + L+ + G RV K L GR+ +++
Sbjct: 1 MATSTVQLAVQSRLLSYGIGESTELVPAPPANRGKRVVCRLRKLELEEMNFSGRSRKIDQ 60
Query: 326 VAIKGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEA 505
K RT R++ L+ S + + QT K++ TY FRT+ GG VKV++ K NGKY V +E
Sbjct: 61 EVSK---RTHRLKALSASGISLIQTAKRVPTYLFRTDIGGQVKVLVEKT-NGKYKVLVEV 116
Query: 506 SSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLS------DSGIFETPFVNNLLNKLSVELE 667
+LS ++VM WG+FRSD+S MPL L+ S ETPFV +K++VEL+
Sbjct: 117 LPSELSDAHAELVMVWGLFRSDASCFMPLDLNRHGADGKSSTIETPFVQGPSDKVTVELD 176
Query: 668 FDAS 679
F+AS
Sbjct: 177 FEAS 180
>ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber]
ref|XP_023886632.1| isoamylase 2, chloroplastic [Quercus suber]
gb|POE68275.1| isoamylase 2, chloroplastic [Quercus suber]
Length = 887
Score = 101 bits (252), Expect = 5e-21
Identities = 75/194 (38%), Positives = 101/194 (52%), Gaps = 23/194 (11%)
Frame = +2
Query: 164 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTR--VTQNFGKNNLGRNLVLNEVA-- 331
MA L S A Q L CG +S S C Y R + F K +L R L+ +EV+
Sbjct: 1 MATLPPSLAIQSGYLKCGISDVSKS--SPACHYIYRNHIRYGFEKMDLERRLICDEVSQN 58
Query: 332 -IKGSYRTRRIQVLATSTVPVTQTVKKLST------------YSFRTEKGGNVKVVITKL 472
+K S R +V ATS V V + +K+ST Y F TE GG VKV + K
Sbjct: 59 VVKSSCRNLNSRVFATSRVLVEEIEQKVSTITEADNREKALTYLFWTEVGGQVKVFVRKT 118
Query: 473 KNGKYGVNIEASSLQLSGNDDDVVMSWGIFRSDSSSLMPLQLSDS------GIFETPFVN 634
+ KY VNIE SSLQL ++D ++ SWG +R+DSSS +PL + S ETPF+
Sbjct: 119 -SVKYAVNIEVSSLQLQSSEDRLISSWGRYRADSSSFIPLDVQSSVPDSRTPTSETPFIQ 177
Query: 635 NLLNKLSVELEFDA 676
+ ++ELEF+A
Sbjct: 178 TSAGRFALELEFEA 191