BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00008809
         (352 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_023762212.1| protein phosphatase 2C 37-like [Lactuca sativa]   147   2e-40
gb|PLY86731.1| hypothetical protein LSAT_2X118601 [Lactuca sativa]    147   3e-40
gb|KVI09878.1| Protein phosphatase 2C [Cynara cardunculus var. s...   138   9e-37
ref|XP_021971888.1| protein phosphatase 2C 37-like [Helianthus a...   129   3e-35
ref|XP_021990009.1| protein phosphatase 2C 37-like [Helianthus a...   133   4e-35
ref|XP_021971890.1| protein phosphatase 2C 37-like [Helianthus a...   128   2e-33
ref|XP_021971889.1| protein phosphatase 2C 37-like [Helianthus a...   127   6e-33
gb|KVI06988.1| Protein phosphatase 2C [Cynara cardunculus var. s...   127   2e-32
ref|XP_023763926.1| protein phosphatase 2C 37-like [Lactuca sati...   126   3e-32
gb|OTF86530.1| putative protein phosphatase 2C 49 [Helianthus an...   119   4e-30
ref|XP_021981867.1| protein phosphatase 2C 37-like [Helianthus a...   115   3e-28
gb|PHU18236.1| Protein phosphatase 2C 37 [Capsicum chinense]          114   3e-28
gb|PHT82155.1| Protein phosphatase 2C 3 [Capsicum annuum]             114   1e-27
gb|PHT48658.1| Protein phosphatase 2C 37 [Capsicum baccatum]          112   4e-27
emb|CAC10359.1| protein phosphatase 2C, partial [Nicotiana tabacum]   112   5e-27
ref|NP_001311930.1| protein phosphatase 2C 37 [Capsicum annuum] ...   112   6e-27
ref|XP_009802038.1| PREDICTED: protein phosphatase 2C 37-like [N...   112   7e-27
ref|NP_001312221.1| protein phosphatase 2C 37-like [Nicotiana ta...   112   7e-27
ref|XP_020228370.1| protein phosphatase 2C 37-like [Cajanus caja...   106   7e-26
ref|XP_019228734.1| PREDICTED: protein phosphatase 2C 37-like [N...   108   1e-25

>ref|XP_023762212.1| protein phosphatase 2C 37-like [Lactuca sativa]
          Length = 420

 Score =  147 bits (372), Expect = 2e-40
 Identities = 71/89 (79%), Positives = 78/89 (87%), Gaps = 3/89 (3%)
 Frame = +3

Query: 93  GTSSETIVEDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFV---NRDRDDSHFYGVYDG 263
           GTSSET+ EDV DKENSRFGLTVVCGRRRDLEDAVAVKP+F    +R  DD HFYGVYDG
Sbjct: 106 GTSSETLGEDVLDKENSRFGLTVVCGRRRDLEDAVAVKPSFCRTSSRSSDDLHFYGVYDG 165

Query: 264 HGCSHVAMKCKERMHEIVKDEVEKCEETM 350
           HGCSHVAMKCK+RMHEIVK+EV  CE++M
Sbjct: 166 HGCSHVAMKCKDRMHEIVKEEVVSCEKSM 194


>gb|PLY86731.1| hypothetical protein LSAT_2X118601 [Lactuca sativa]
          Length = 432

 Score =  147 bits (372), Expect = 3e-40
 Identities = 71/89 (79%), Positives = 78/89 (87%), Gaps = 3/89 (3%)
 Frame = +3

Query: 93  GTSSETIVEDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFV---NRDRDDSHFYGVYDG 263
           GTSSET+ EDV DKENSRFGLTVVCGRRRDLEDAVAVKP+F    +R  DD HFYGVYDG
Sbjct: 118 GTSSETLGEDVLDKENSRFGLTVVCGRRRDLEDAVAVKPSFCRTSSRSSDDLHFYGVYDG 177

Query: 264 HGCSHVAMKCKERMHEIVKDEVEKCEETM 350
           HGCSHVAMKCK+RMHEIVK+EV  CE++M
Sbjct: 178 HGCSHVAMKCKDRMHEIVKEEVVSCEKSM 206


>gb|KVI09878.1| Protein phosphatase 2C [Cynara cardunculus var. scolymus]
          Length = 427

 Score =  138 bits (348), Expect = 9e-37
 Identities = 74/118 (62%), Positives = 88/118 (74%), Gaps = 3/118 (2%)
 Frame = +3

Query: 6   KVDTVQYSGTETTPKVCHDSVDIKRDVLVGTSSETIVEDVSDKENSRFGLTVVCGRRRDL 185
           K++ V  SGT         S + KR    G S ET+  DVSD+ENSRFGLTVVCGRRRDL
Sbjct: 93  KLEEVGSSGT---------SSETKRLNRCGASLETLDRDVSDEENSRFGLTVVCGRRRDL 143

Query: 186 EDAVAVKPAFV---NRDRDDSHFYGVYDGHGCSHVAMKCKERMHEIVKDEVEKCEETM 350
           EDAVAVKP+F    +++ +D HFYGVYDGHGCSHVAMKCK+RMHEIVK+EVE  E++M
Sbjct: 144 EDAVAVKPSFCKTSSQNCNDLHFYGVYDGHGCSHVAMKCKDRMHEIVKEEVENGEKSM 201


>ref|XP_021971888.1| protein phosphatase 2C 37-like [Helianthus annuus]
 gb|OTG21205.1| putative PPM-type phosphatase domain, Protein phosphatase 2C family
           [Helianthus annuus]
          Length = 221

 Score =  129 bits (325), Expect = 3e-35
 Identities = 58/83 (69%), Positives = 71/83 (85%)
 Frame = +3

Query: 96  TSSETIVEDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFVNRDRDDSHFYGVYDGHGCS 275
           T+S++  +DV +KE+SRFGLT+VCGRRRDLEDAV+VKP+F      D HFYGV+DGHGCS
Sbjct: 61  TTSKSFRDDVLEKESSRFGLTIVCGRRRDLEDAVSVKPSFCKMICSDLHFYGVFDGHGCS 120

Query: 276 HVAMKCKERMHEIVKDEVEKCEE 344
           HVA KCK+RMHEIVK+EVE CE+
Sbjct: 121 HVATKCKDRMHEIVKEEVENCED 143


>ref|XP_021990009.1| protein phosphatase 2C 37-like [Helianthus annuus]
 gb|OTG12724.1| putative PPM-type phosphatase domain, Protein phosphatase 2C family
           [Helianthus annuus]
          Length = 395

 Score =  133 bits (335), Expect = 4e-35
 Identities = 63/81 (77%), Positives = 70/81 (86%), Gaps = 3/81 (3%)
 Frame = +3

Query: 117 EDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFVNRDR---DDSHFYGVYDGHGCSHVAM 287
           E+V DKE SRFGLTVVCGRRRDLEDAVAVKP+F   +    DD HFYGVYDGHGCSHVAM
Sbjct: 85  EEVLDKEGSRFGLTVVCGRRRDLEDAVAVKPSFCKTNSSVSDDLHFYGVYDGHGCSHVAM 144

Query: 288 KCKERMHEIVKDEVEKCEETM 350
           KCK+RMHEIVK+EVE CEE++
Sbjct: 145 KCKDRMHEIVKEEVENCEESI 165


>ref|XP_021971890.1| protein phosphatase 2C 37-like [Helianthus annuus]
 gb|OTG21207.1| putative PPM-type phosphatase domain, Protein phosphatase 2C family
           [Helianthus annuus]
          Length = 355

 Score =  128 bits (322), Expect = 2e-33
 Identities = 60/87 (68%), Positives = 71/87 (81%)
 Frame = +3

Query: 90  VGTSSETIVEDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFVNRDRDDSHFYGVYDGHG 269
           V TS ++  +DV +KE+SRFGLTVVCGRRRDLEDAV+VKP+F        HFYGV+DGHG
Sbjct: 41  VTTSKKSFRDDVLEKESSRFGLTVVCGRRRDLEDAVSVKPSFCKTICSYLHFYGVFDGHG 100

Query: 270 CSHVAMKCKERMHEIVKDEVEKCEETM 350
           CSHVA KCK+RMHEIVK+EVE CEE +
Sbjct: 101 CSHVATKCKDRMHEIVKEEVENCEEAI 127


>ref|XP_021971889.1| protein phosphatase 2C 37-like [Helianthus annuus]
 gb|OTG21206.1| putative PPM-type phosphatase domain, Protein phosphatase 2C family
           [Helianthus annuus]
          Length = 369

 Score =  127 bits (319), Expect = 6e-33
 Identities = 57/83 (68%), Positives = 71/83 (85%)
 Frame = +3

Query: 96  TSSETIVEDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFVNRDRDDSHFYGVYDGHGCS 275
           T+S++  +DV +KE+SRFGLT+VCGRRRDLEDAV+VKP+F      D HFYGV+DGHGCS
Sbjct: 61  TTSKSFRDDVLEKESSRFGLTIVCGRRRDLEDAVSVKPSFCKMICSDLHFYGVFDGHGCS 120

Query: 276 HVAMKCKERMHEIVKDEVEKCEE 344
           HVA KCK+RM+EIVK+EVE CE+
Sbjct: 121 HVARKCKDRMYEIVKEEVENCED 143


>gb|KVI06988.1| Protein phosphatase 2C [Cynara cardunculus var. scolymus]
          Length = 416

 Score =  127 bits (318), Expect = 2e-32
 Identities = 71/140 (50%), Positives = 90/140 (64%), Gaps = 24/140 (17%)
 Frame = +3

Query: 3   RKVDTVQYSGTE------TTPKVCHDSVD-----IKRDVLVGTSSET------------- 110
           RKV++++  G        TTP  C   V      +K +  +G+SSET             
Sbjct: 55  RKVESLEMIGNNVDAKALTTPLECEKVVQPCHMKLKEEGHLGSSSETETVLNPCKLLSET 114

Query: 111 IVEDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFVNRDRDDSHFYGVYDGHGCSHVAMK 290
           + E V D+E+SRFG+T VCGRRRD+EDAVAVKP+F +    D HFYGVYDGHGCSHVAMK
Sbjct: 115 VGETVRDEESSRFGITTVCGRRRDMEDAVAVKPSFSS----DLHFYGVYDGHGCSHVAMK 170

Query: 291 CKERMHEIVKDEVEKCEETM 350
           CK+RMHEIVK+EVE   E++
Sbjct: 171 CKDRMHEIVKEEVENSGESL 190


>ref|XP_023763926.1| protein phosphatase 2C 37-like [Lactuca sativa]
 gb|PLY85395.1| hypothetical protein LSAT_9X63741 [Lactuca sativa]
          Length = 420

 Score =  126 bits (317), Expect = 3e-32
 Identities = 59/82 (71%), Positives = 69/82 (84%)
 Frame = +3

Query: 105 ETIVEDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFVNRDRDDSHFYGVYDGHGCSHVA 284
           ET+VE V D++ SRFG+T VCGRRRD+EDAVAVKP+F     DD HFYGVYDGHGCSHVA
Sbjct: 116 ETVVETVQDEDESRFGVTTVCGRRRDMEDAVAVKPSFCG---DDLHFYGVYDGHGCSHVA 172

Query: 285 MKCKERMHEIVKDEVEKCEETM 350
           MKCK+RMHEIVK+EVE   E++
Sbjct: 173 MKCKDRMHEIVKNEVENGGESL 194


>gb|OTF86530.1| putative protein phosphatase 2C 49 [Helianthus annuus]
          Length = 360

 Score =  119 bits (299), Expect = 4e-30
 Identities = 56/90 (62%), Positives = 69/90 (76%), Gaps = 3/90 (3%)
 Frame = +3

Query: 90  VGTSSETIVEDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFVNRDRD---DSHFYGVYD 260
           V TS ++  +DV +KE+SRFGL +VCGR RDLED V+VK +F   +     D HFYGV+D
Sbjct: 41  VTTSKKSFGDDVLEKESSRFGLNIVCGRMRDLEDVVSVKSSFCKTNASVCSDLHFYGVFD 100

Query: 261 GHGCSHVAMKCKERMHEIVKDEVEKCEETM 350
           GHGCSHVA KCK+RMHEIVK+EVE CEE +
Sbjct: 101 GHGCSHVATKCKDRMHEIVKEEVENCEEAI 130


>ref|XP_021981867.1| protein phosphatase 2C 37-like [Helianthus annuus]
 gb|OTG14495.1| putative protein phosphatase 2CA [Helianthus annuus]
          Length = 385

 Score =  115 bits (288), Expect = 3e-28
 Identities = 54/83 (65%), Positives = 66/83 (79%)
 Frame = +3

Query: 102 SETIVEDVSDKENSRFGLTVVCGRRRDLEDAVAVKPAFVNRDRDDSHFYGVYDGHGCSHV 281
           +E + + V D   +RFG+T VCGRRRD+EDAVAVKP+F +    D +FYGVYDGHGCSHV
Sbjct: 81  TEAVSKTVCDNNEARFGVTTVCGRRRDMEDAVAVKPSFSS----DFNFYGVYDGHGCSHV 136

Query: 282 AMKCKERMHEIVKDEVEKCEETM 350
           AMKCK+RMHEIVKDEVE   E++
Sbjct: 137 AMKCKDRMHEIVKDEVETAGESV 159


>gb|PHU18236.1| Protein phosphatase 2C 37 [Capsicum chinense]
          Length = 317

 Score =  114 bits (284), Expect = 3e-28
 Identities = 59/116 (50%), Positives = 79/116 (68%), Gaps = 5/116 (4%)
 Frame = +3

Query: 3   RKVDTVQYSGTETTPKVCHDSVDIKRDVLV-GTSSETIVE-DVSDKENSRFGLTVVCGRR 176
           +K+++       T+P V  D  ++  D+   GT+    +E +V   +  +FG+T VCGRR
Sbjct: 71  QKLESTVTISLSTSPVVVSDEKEVVPDLSESGTTQLGYMENEVVSDDLPKFGMTSVCGRR 130

Query: 177 RDLEDAVAVKPAFVNRDRDDS---HFYGVYDGHGCSHVAMKCKERMHEIVKDEVEK 335
           RD+EDAVA+ P+F   + + S   HFYGVYDGHGCSHVAMKCK+RMHEIVKDEVEK
Sbjct: 131 RDMEDAVAIHPSFTKENSETSRNLHFYGVYDGHGCSHVAMKCKDRMHEIVKDEVEK 186


>gb|PHT82155.1| Protein phosphatase 2C 3 [Capsicum annuum]
          Length = 420

 Score =  114 bits (284), Expect = 1e-27
 Identities = 59/116 (50%), Positives = 79/116 (68%), Gaps = 5/116 (4%)
 Frame = +3

Query: 3   RKVDTVQYSGTETTPKVCHDSVDIKRDVLV-GTSSETIVE-DVSDKENSRFGLTVVCGRR 176
           +K+++       T+P V  D  ++  D+   GT+    +E +V   +  +FG+T VCGRR
Sbjct: 71  QKLESTVTISLSTSPVVVSDEKEVVPDLSESGTTQLGYMENEVVSDDLPKFGMTSVCGRR 130

Query: 177 RDLEDAVAVKPAFVNRDRDDS---HFYGVYDGHGCSHVAMKCKERMHEIVKDEVEK 335
           RD+EDAVA+ P+F   + + S   HFYGVYDGHGCSHVAMKCK+RMHEIVKDEVEK
Sbjct: 131 RDMEDAVAIHPSFTKENSETSRNLHFYGVYDGHGCSHVAMKCKDRMHEIVKDEVEK 186


>gb|PHT48658.1| Protein phosphatase 2C 37 [Capsicum baccatum]
          Length = 420

 Score =  112 bits (281), Expect = 4e-27
 Identities = 58/116 (50%), Positives = 79/116 (68%), Gaps = 5/116 (4%)
 Frame = +3

Query: 3   RKVDTVQYSGTETTPKVCHDSVDIKRDVLV-GTSSETIVE-DVSDKENSRFGLTVVCGRR 176
           +K+++       T+P V  D  ++  D+   GT+    +E +V   +  +FG+T VCGRR
Sbjct: 71  QKLESTVTISLSTSPVVVSDEKEVVPDLSESGTTQLGYMENEVVSDDLPKFGMTSVCGRR 130

Query: 177 RDLEDAVAVKPAFVNRDRDDS---HFYGVYDGHGCSHVAMKCKERMHEIVKDEVEK 335
           RD+EDAVA+ P+F   + + S   HFYGVYDGHGCSHVAMKCK+RMHEIVKDEV+K
Sbjct: 131 RDMEDAVAIHPSFTKENSETSRNLHFYGVYDGHGCSHVAMKCKDRMHEIVKDEVDK 186


>emb|CAC10359.1| protein phosphatase 2C, partial [Nicotiana tabacum]
          Length = 378

 Score =  112 bits (279), Expect = 5e-27
 Identities = 61/108 (56%), Positives = 72/108 (66%), Gaps = 8/108 (7%)
 Frame = +3

Query: 42  TPKVCHDSVDIKR-DVLVGTSSETIVEDVS----DKENSRFGLTVVCGRRRDLEDAVAVK 206
           T KV     D K  D+     S   V+ V     D   S+FG+T VCGRRRD+EDAVAV 
Sbjct: 78  TEKVAEKEFDAKPLDLSESDVSSVKVDGVEVVAGDNNLSKFGITSVCGRRRDMEDAVAVH 137

Query: 207 PAFVNRDRDDS---HFYGVYDGHGCSHVAMKCKERMHEIVKDEVEKCE 341
           P+F   + ++S   HFYGVYDGHGCSHVAMKCK+RMHEIVK+EVEK E
Sbjct: 138 PSFCKGENENSNSLHFYGVYDGHGCSHVAMKCKDRMHEIVKNEVEKGE 185


>ref|NP_001311930.1| protein phosphatase 2C 37 [Capsicum annuum]
 gb|AML80890.1| 2C type protein phosphatase [Capsicum annuum]
          Length = 420

 Score =  112 bits (280), Expect = 6e-27
 Identities = 58/116 (50%), Positives = 78/116 (67%), Gaps = 5/116 (4%)
 Frame = +3

Query: 3   RKVDTVQYSGTETTPKVCHDSVDIKRDVLV-GTSSETIVE-DVSDKENSRFGLTVVCGRR 176
           +K+++       T+P V  D  ++  D+   GT+    +E +V   +  +FG+T VCGRR
Sbjct: 71  QKLESTVTISLSTSPVVVSDEKEVVPDLSESGTTQLGYMENEVVSDDLPKFGMTSVCGRR 130

Query: 177 RDLEDAVAVKPAFVNRDRDDS---HFYGVYDGHGCSHVAMKCKERMHEIVKDEVEK 335
           RD+ED VA+ P+F   + + S   HFYGVYDGHGCSHVAMKCK+RMHEIVKDEVEK
Sbjct: 131 RDMEDTVAIHPSFTKENSETSRNLHFYGVYDGHGCSHVAMKCKDRMHEIVKDEVEK 186


>ref|XP_009802038.1| PREDICTED: protein phosphatase 2C 37-like [Nicotiana sylvestris]
          Length = 416

 Score =  112 bits (279), Expect = 7e-27
 Identities = 61/108 (56%), Positives = 72/108 (66%), Gaps = 8/108 (7%)
 Frame = +3

Query: 42  TPKVCHDSVDIKR-DVLVGTSSETIVEDVS----DKENSRFGLTVVCGRRRDLEDAVAVK 206
           T KV     D K  D+     S   V+ V     D   S+FG+T VCGRRRD+EDAVAV 
Sbjct: 78  TEKVAEKEFDAKPLDLSESDVSSVKVDGVEVVAGDNNLSKFGITSVCGRRRDMEDAVAVH 137

Query: 207 PAFVNRDRDDS---HFYGVYDGHGCSHVAMKCKERMHEIVKDEVEKCE 341
           P+F   + ++S   HFYGVYDGHGCSHVAMKCK+RMHEIVK+EVEK E
Sbjct: 138 PSFCKGENENSNSLHFYGVYDGHGCSHVAMKCKDRMHEIVKNEVEKGE 185


>ref|NP_001312221.1| protein phosphatase 2C 37-like [Nicotiana tabacum]
 dbj|BAF43700.1| protein phosphatase 2C [Nicotiana tabacum]
          Length = 416

 Score =  112 bits (279), Expect = 7e-27
 Identities = 61/108 (56%), Positives = 72/108 (66%), Gaps = 8/108 (7%)
 Frame = +3

Query: 42  TPKVCHDSVDIKR-DVLVGTSSETIVEDVS----DKENSRFGLTVVCGRRRDLEDAVAVK 206
           T KV     D K  D+     S   V+ V     D   S+FG+T VCGRRRD+EDAVAV 
Sbjct: 78  TEKVAEKEFDAKPLDLSESDVSSVKVDGVEVVAGDNNLSKFGITSVCGRRRDMEDAVAVH 137

Query: 207 PAFVNRDRDDS---HFYGVYDGHGCSHVAMKCKERMHEIVKDEVEKCE 341
           P+F   + ++S   HFYGVYDGHGCSHVAMKCK+RMHEIVK+EVEK E
Sbjct: 138 PSFCKGENENSNSLHFYGVYDGHGCSHVAMKCKDRMHEIVKNEVEKGE 185


>ref|XP_020228370.1| protein phosphatase 2C 37-like [Cajanus cajan]
 gb|KYP72626.1| Protein phosphatase 2C 37 [Cajanus cajan]
          Length = 263

 Score =  106 bits (265), Expect = 7e-26
 Identities = 59/130 (45%), Positives = 80/130 (61%), Gaps = 14/130 (10%)
 Frame = +3

Query: 3   RKVDTVQYSGTETTPKVCHDSVDIKRDVLVGTSSETIVEDVSDKENS----------RFG 152
           RK   +  SG+   P+ C ++V+        + S+ +VE VS    S          R+G
Sbjct: 51  RKRQKLDASGSPAQPRECQNAVE-------SSESKGVVEAVSLPNTSKIPAFVEECPRYG 103

Query: 153 LTVVCGRRRDLEDAVAVKPAFV--NRDRDDS--HFYGVYDGHGCSHVAMKCKERMHEIVK 320
           +T VCGRRRD+EDAV+V P+F   NR +D    HF+ V+DGHGCSHVA  CKER+HEIVK
Sbjct: 104 VTSVCGRRRDMEDAVSVHPSFCLENRSQDQKGFHFFAVFDGHGCSHVATMCKERLHEIVK 163

Query: 321 DEVEKCEETM 350
           +EVEK +E +
Sbjct: 164 EEVEKAKEDL 173


>ref|XP_019228734.1| PREDICTED: protein phosphatase 2C 37-like [Nicotiana attenuata]
 gb|OIT30550.1| protein phosphatase 2c 37 [Nicotiana attenuata]
          Length = 416

 Score =  108 bits (271), Expect = 1e-25
 Identities = 51/74 (68%), Positives = 59/74 (79%), Gaps = 3/74 (4%)
 Frame = +3

Query: 129 DKENSRFGLTVVCGRRRDLEDAVAVKPAFVNRDRDDS---HFYGVYDGHGCSHVAMKCKE 299
           D   S+FG T VCGRRRD+EDAVA  P+F   + ++S   HFYGVYDGHGCSHVAMKCK+
Sbjct: 112 DNNPSKFGTTSVCGRRRDMEDAVATHPSFCKGENENSNNLHFYGVYDGHGCSHVAMKCKD 171

Query: 300 RMHEIVKDEVEKCE 341
           RMHEIVK+EVEK E
Sbjct: 172 RMHEIVKNEVEKGE 185