BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00006069
         (2384 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_021983379.1| senescence-associated carboxylesterase 101-l...   603   0.0  
ref|XP_022029823.1| senescence-associated carboxylesterase 101-l...   593   0.0  
gb|OTG32732.1| putative alpha/Beta hydrolase fold protein [Helia...   593   0.0  
ref|XP_023743613.1| senescence-associated carboxylesterase 101-l...   575   0.0  
gb|KVH92757.1| Lipase, class 3, partial [Cynara cardunculus var....   582   0.0  
ref|XP_023743631.1| senescence-associated carboxylesterase 101-l...   556   0.0  
ref|XP_023743645.1| senescence-associated carboxylesterase 101-l...   547   0.0  
ref|XP_021984326.1| senescence-associated carboxylesterase 101-l...   522   e-174
ref|XP_023743620.1| senescence-associated carboxylesterase 101-l...   501   e-166
gb|PLY96564.1| hypothetical protein LSAT_4X180981 [Lactuca sativa]    486   e-161
ref|XP_022029822.1| senescence-associated carboxylesterase 101-l...   481   e-158
gb|OTF95917.1| putative alpha/Beta hydrolase fold protein [Helia...   446   e-144
ref|XP_022014633.1| senescence-associated carboxylesterase 101-l...   395   e-126
gb|KVH92759.1| Lipase, class 3, partial [Cynara cardunculus var....   345   e-108
ref|XP_016510829.1| PREDICTED: senescence-associated carboxylest...   347   e-106
ref|XP_016510827.1| PREDICTED: senescence-associated carboxylest...   346   e-106
ref|XP_019223911.1| PREDICTED: senescence-associated carboxylest...   343   e-105
ref|XP_009764370.1| PREDICTED: senescence-associated carboxylest...   343   e-105
ref|XP_019223910.1| PREDICTED: senescence-associated carboxylest...   342   e-104
ref|XP_009764369.1| PREDICTED: senescence-associated carboxylest...   342   e-104

>ref|XP_021983379.1| senescence-associated carboxylesterase 101-like [Helianthus annuus]
 gb|OTG15932.1| putative alpha/Beta hydrolase fold protein [Helianthus annuus]
          Length = 550

 Score =  603 bits (1555), Expect = 0.0
 Identities = 310/551 (56%), Positives = 400/551 (72%), Gaps = 3/551 (0%)
 Frame = +2

Query: 563  MFSSEVELSNLLGSFDILPDAYQAILKTSSAYQLHTDIQSGIQFLAFN-ISSEYTTRFLK 739
            MF+SEVEL   LGS  ++P+AYQAI +T+ AY+LHT   SG   LAF+  S +YTTRFL 
Sbjct: 5    MFNSEVELGKFLGSIGVIPEAYQAISETTLAYELHTT-PSGNTVLAFSSCSPDYTTRFLN 63

Query: 740  GEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTGRC 919
            G F+LV S+N   VDFISTKV   F IN+AAV+LF+ +  +L ++E +     L++TG  
Sbjct: 64   GGFDLVPSKNFQGVDFISTKVNSSFFINEAAVELFQQLGDDLKELENKHINSSLIITGSG 123

Query: 920  LGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSSF 1099
            LGGYLAIL TL L  AID++E NG    KRPICITFGSPL+G++A + AI ERP WKSSF
Sbjct: 124  LGGYLAILSTLRLHHAIDVEESNGSKKTKRPICITFGSPLIGDKAFKSAIDERPQWKSSF 183

Query: 1100 LNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAENLE 1279
            LNVV++KDP+ASFFSS T YKPFGTFLFC ESGG++  ED D+IL VL++M SS  +N +
Sbjct: 184  LNVVARKDPIASFFSSETRYKPFGTFLFCVESGGHAAFEDQDSILPVLEAMKSSNDDNSQ 243

Query: 1280 IHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEENQ 1459
            ++ Y N L++I  K LYRGVSELGE ++N  RAGITLQL E             +ME+ Q
Sbjct: 244  VYDYTNVLSSIRRKVLYRGVSELGEFNLNPLRAGITLQLKEVDVLSDITNDEIGKMEKKQ 303

Query: 1460 AKTKRRKKDISNH--RNKLSEIKISLIYMEWYMKSRKKYGGYYDSYKVQSTREEIVSHRV 1633
                +RK  +S +    KL+++KISL YMEWYMK+RK  GGYYDSYK  ++  +I   ++
Sbjct: 304  TDMIKRKSAMSAYDPTKKLNDMKISLTYMEWYMKTRKSEGGYYDSYKNATSTSDIEKQQL 363

Query: 1634 IYTHKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSN 1813
            +  H+  LNQ+W+K V E ++MP +E AK+RK WL   TNYRRIVEPLDIA+HYKKGN+N
Sbjct: 364  MTKHQRILNQYWKKVVEEKDIMPQKEGAKLRKRWLFGGTNYRRIVEPLDIAEHYKKGNTN 423

Query: 1814 YTEQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEG 1993
            Y + RP+HYKLLEKW +ED++D   SE    KAA+LT+D+CFWAHVEEALI L DL N G
Sbjct: 424  YIKNRPDHYKLLEKWSDEDKKDSKTSEVTRHKAASLTEDSCFWAHVEEALISLKDLVNGG 483

Query: 1994 SSDNIDGILLGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYASEFAQ 2173
            S++N +     L+ FE+Y+M  IN Y VS DIF++GSSLM+WWN YK ++G+ YASEFA+
Sbjct: 484  STENEE----KLKEFESYMMSAINNYSVSQDIFLEGSSLMKWWNGYKAHKGTGYASEFAE 539

Query: 2174 YMNSKSYTSYQ 2206
            YMN++ Y SYQ
Sbjct: 540  YMNNEGYRSYQ 550


>ref|XP_022029823.1| senescence-associated carboxylesterase 101-like [Helianthus annuus]
          Length = 547

 Score =  593 bits (1528), Expect = 0.0
 Identities = 315/550 (57%), Positives = 391/550 (71%), Gaps = 2/550 (0%)
 Frame = +2

Query: 563  MFSSEVELSNLLGSFDILPDAYQAILKTSSAYQLHTDIQSGIQFLAFN-ISSEYTTRFLK 739
            MF++E+EL   LGS D++PDAYQAI +T+ AY+LHT  +SG   LAF+    +YTTRFL 
Sbjct: 5    MFNNEIELGKFLGSIDVIPDAYQAISETTLAYELHTT-RSGNTVLAFSSCPPDYTTRFLT 63

Query: 740  GEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTGRC 919
            GEF+LVSS N  VVDFI TKV   FSINKAAV+LF+ +  +L ++E++     L++TG  
Sbjct: 64   GEFDLVSSENLQVVDFIPTKVNSSFSINKAAVELFQQLGDDLKELEHKYINSSLIITGSG 123

Query: 920  LGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSSF 1099
            LGGYLAIL  L L  AID++E NG    KRPICITFGSPL+G++A + AI ERP WK SF
Sbjct: 124  LGGYLAILSALRLHHAIDVEESNGSKKTKRPICITFGSPLIGDKAFKSAIDERPQWKFSF 183

Query: 1100 LNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAENLE 1279
            LNVV++KDPVA+FFSS T YKPFGTFLFC ESGG++  ED  +IL VLD+M  S  ++ +
Sbjct: 184  LNVVARKDPVATFFSSNTLYKPFGTFLFCTESGGHTAFEDQHSILPVLDAMKFSNEDSSQ 243

Query: 1280 IHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEENQ 1459
            ++ Y N L++I  K LYRG SEL E ++NS RA I LQL E             +M ENQ
Sbjct: 244  VYDYTNVLSSIRRKVLYRGSSELDEFNLNSLRAEIKLQLKEVDVLSDITNDDIGKM-ENQ 302

Query: 1460 AKTKRRKKDISNHRNKLSEIKISLIYMEWYMKSRKKYG-GYYDSYKVQSTREEIVSHRVI 1636
            AK  +RKK+      KL+E+KISL YMEWYM  +K+ G GYYD YK   TREEIV  + I
Sbjct: 303  AKLNKRKKNPYEPTKKLNEMKISLTYMEWYMTGQKRKGCGYYDGYKNPKTREEIVGQQEI 362

Query: 1637 YTHKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNY 1816
              H+ +LNQ+W+  V E + MP +E AK+RK WL    NYR+IVEPLDIA++YKKGN  Y
Sbjct: 363  LKHQGRLNQYWKDIVVEKDRMPQKEGAKLRKRWLLGGNNYRKIVEPLDIAEYYKKGNIKY 422

Query: 1817 TEQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGS 1996
             E R NHYKLLEKWL+ED +D N SE    KAA+LT+D+CFWAHVEEALILL ++ N G 
Sbjct: 423  IENRSNHYKLLEKWLDED-KDSNSSEVTRNKAASLTEDSCFWAHVEEALILLRNMVN-GG 480

Query: 1997 SDNIDGILLGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYASEFAQY 2176
            S N D     LE FE Y+M EI  Y VSPDIF+ GSSLM WWNEYK Y+G+ YASEFA+Y
Sbjct: 481  SINAD---KELEEFEVYVMHEIENYTVSPDIFLKGSSLMTWWNEYKAYKGTAYASEFARY 537

Query: 2177 MNSKSYTSYQ 2206
            MN+ SY SY+
Sbjct: 538  MNNASYDSYK 547


>gb|OTG32732.1| putative alpha/Beta hydrolase fold protein [Helianthus annuus]
          Length = 593

 Score =  593 bits (1528), Expect = 0.0
 Identities = 315/550 (57%), Positives = 391/550 (71%), Gaps = 2/550 (0%)
 Frame = +2

Query: 563  MFSSEVELSNLLGSFDILPDAYQAILKTSSAYQLHTDIQSGIQFLAFN-ISSEYTTRFLK 739
            MF++E+EL   LGS D++PDAYQAI +T+ AY+LHT  +SG   LAF+    +YTTRFL 
Sbjct: 51   MFNNEIELGKFLGSIDVIPDAYQAISETTLAYELHTT-RSGNTVLAFSSCPPDYTTRFLT 109

Query: 740  GEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTGRC 919
            GEF+LVSS N  VVDFI TKV   FSINKAAV+LF+ +  +L ++E++     L++TG  
Sbjct: 110  GEFDLVSSENLQVVDFIPTKVNSSFSINKAAVELFQQLGDDLKELEHKYINSSLIITGSG 169

Query: 920  LGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSSF 1099
            LGGYLAIL  L L  AID++E NG    KRPICITFGSPL+G++A + AI ERP WK SF
Sbjct: 170  LGGYLAILSALRLHHAIDVEESNGSKKTKRPICITFGSPLIGDKAFKSAIDERPQWKFSF 229

Query: 1100 LNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAENLE 1279
            LNVV++KDPVA+FFSS T YKPFGTFLFC ESGG++  ED  +IL VLD+M  S  ++ +
Sbjct: 230  LNVVARKDPVATFFSSNTLYKPFGTFLFCTESGGHTAFEDQHSILPVLDAMKFSNEDSSQ 289

Query: 1280 IHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEENQ 1459
            ++ Y N L++I  K LYRG SEL E ++NS RA I LQL E             +M ENQ
Sbjct: 290  VYDYTNVLSSIRRKVLYRGSSELDEFNLNSLRAEIKLQLKEVDVLSDITNDDIGKM-ENQ 348

Query: 1460 AKTKRRKKDISNHRNKLSEIKISLIYMEWYMKSRKKYG-GYYDSYKVQSTREEIVSHRVI 1636
            AK  +RKK+      KL+E+KISL YMEWYM  +K+ G GYYD YK   TREEIV  + I
Sbjct: 349  AKLNKRKKNPYEPTKKLNEMKISLTYMEWYMTGQKRKGCGYYDGYKNPKTREEIVGQQEI 408

Query: 1637 YTHKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNY 1816
              H+ +LNQ+W+  V E + MP +E AK+RK WL    NYR+IVEPLDIA++YKKGN  Y
Sbjct: 409  LKHQGRLNQYWKDIVVEKDRMPQKEGAKLRKRWLLGGNNYRKIVEPLDIAEYYKKGNIKY 468

Query: 1817 TEQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGS 1996
             E R NHYKLLEKWL+ED +D N SE    KAA+LT+D+CFWAHVEEALILL ++ N G 
Sbjct: 469  IENRSNHYKLLEKWLDED-KDSNSSEVTRNKAASLTEDSCFWAHVEEALILLRNMVN-GG 526

Query: 1997 SDNIDGILLGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYASEFAQY 2176
            S N D     LE FE Y+M EI  Y VSPDIF+ GSSLM WWNEYK Y+G+ YASEFA+Y
Sbjct: 527  SINAD---KELEEFEVYVMHEIENYTVSPDIFLKGSSLMTWWNEYKAYKGTAYASEFARY 583

Query: 2177 MNSKSYTSYQ 2206
            MN+ SY SY+
Sbjct: 584  MNNASYDSYK 593


>ref|XP_023743613.1| senescence-associated carboxylesterase 101-like [Lactuca sativa]
 gb|PLY96558.1| hypothetical protein LSAT_4X180921 [Lactuca sativa]
          Length = 545

 Score =  575 bits (1483), Expect = 0.0
 Identities = 304/548 (55%), Positives = 379/548 (69%)
 Frame = +2

Query: 560  EMFSSEVELSNLLGSFDILPDAYQAILKTSSAYQLHTDIQSGIQFLAFNISSEYTTRFLK 739
            +MF+SEVE+   LGS  ++ DAY+AI +TSS YQLHT   SGI+ LAFN  S+YT  F K
Sbjct: 4    KMFNSEVEVGKFLGSIHVIQDAYRAISQTSSTYQLHTS-PSGIKVLAFNCLSDYTIPFRK 62

Query: 740  GEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTGRC 919
            GE +LVSS NH VV+FISTKV P  SINKAAV+LFE    ELS++  QL    LVVTG  
Sbjct: 63   GE-DLVSSDNHKVVEFISTKVNPKISINKAAVELFELHFNELSELSNQLINDQLVVTGCG 121

Query: 920  LGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSSF 1099
            L GYLAIL+TL  Q   D+KE NG    KRPICITFGSPL+GN+ LQ AI ERP WKSSF
Sbjct: 122  LAGYLAILYTLLHQHYADLKESNGSKTAKRPICITFGSPLIGNQHLQGAISERPQWKSSF 181

Query: 1100 LNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAENLE 1279
            LNVV+K D +ASFFSS + YKPFGTFLFC ESGG++  ED D ILAVLD+M S  A N +
Sbjct: 182  LNVVAKTDLLASFFSSTSQYKPFGTFLFCTESGGHTAFEDQDAILAVLDAMVSPNAGNYQ 241

Query: 1280 IHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEENQ 1459
            +H Y  EL +I    LYRG S+  E ++   RAGI  Q  E             +ME+ Q
Sbjct: 242  MHDYSKELGSIRKTVLYRGGSKFKESNLTLLRAGIVFQFQEIGALNDISNDLIEKMEKKQ 301

Query: 1460 AKTKRRKKDISNHRNKLSEIKISLIYMEWYMKSRKKYGGYYDSYKVQSTREEIVSHRVIY 1639
             +   + +++     KL+++KI L Y+EWYMK+R+  GGYYDSYK   +  E+       
Sbjct: 302  TRMIIKSRNVYEPTKKLNDMKIILTYIEWYMKTRRLTGGYYDSYKNAESTNELKGKNEAI 361

Query: 1640 THKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYT 1819
             H+LKLNQ+W+KTV EN+L+P +E AK+RK WL + TNYRRIVEPLDIADHYK+G +NY 
Sbjct: 362  IHQLKLNQYWKKTVEENDLVPQKEGAKLRKRWLYAGTNYRRIVEPLDIADHYKRGKTNYM 421

Query: 1820 EQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSS 1999
              RPNHYKLLEKW  E+++   PS+ +  KAA+LT+D+CFWAHVEEALI L DLRN G  
Sbjct: 422  AIRPNHYKLLEKWSEEEKKGRKPSDPK-TKAASLTEDSCFWAHVEEALISLRDLRN-GDP 479

Query: 2000 DNIDGILLGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYASEFAQYM 2179
            +N   I   +E FE Y+   I  + VSP+IF++GSS M+WW+EYK Y+GS Y S  A+YM
Sbjct: 480  NN---IATDIEKFEVYVWHSIKDFSVSPEIFVEGSSFMKWWSEYKGYKGSSYDSVLARYM 536

Query: 2180 NSKSYTSY 2203
            N K+Y SY
Sbjct: 537  NDKNYISY 544


>gb|KVH92757.1| Lipase, class 3, partial [Cynara cardunculus var. scolymus]
          Length = 1132

 Score =  582 bits (1500), Expect = 0.0
 Identities = 299/560 (53%), Positives = 382/560 (68%), Gaps = 14/560 (2%)
 Frame = +2

Query: 566  FSSEVELSNLLGSFDILPDAYQAILKTSSA---YQLHTDIQSGIQFLAFNISSEYTTRFL 736
            F SEVE+   +GS D++P+A++A+ +TSS+   Y  HT   SGIQ LAFN S +Y  RFL
Sbjct: 21   FCSEVEVGGFVGSIDVVPEAFRAVSETSSSSSSYGFHTT-SSGIQILAFNCSIDYRNRFL 79

Query: 737  KGEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTGR 916
             G F+LVSS    VVDFI TKV P F I++ AV+LFE++  EL Q++ +    PL+VTG 
Sbjct: 80   SGGFDLVSSETFKVVDFIGTKVNPSFFISEVAVELFESLPLELKQLKDE----PLIVTGL 135

Query: 917  CLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSS 1096
             LGGY+A+LF LWLQ A+D+KE +G  + KRPICITFG PL+G+EALQ ++ ERP WKS 
Sbjct: 136  SLGGYIAVLFALWLQHAVDVKEASGSKDTKRPICITFGCPLVGDEALQRSVSERPQWKSG 195

Query: 1097 FLNVVSKKDPVASFFSSYT----PYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSI 1264
            FLNVV+K DPVASF SS      PYKPFGTFLFC ESGG++  ED +++LAVL +M SS 
Sbjct: 196  FLNVVAKTDPVASFISSKIQAERPYKPFGTFLFCTESGGHTAFEDQESVLAVLHAMASSR 255

Query: 1265 AENLEIHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXR 1444
              N+E+  Y N L AI  K LYRGVSE G+ ++N  RAGIT Q  E             R
Sbjct: 256  HGNMEVPDYENHLTAIRRKALYRGVSEFGQFNLNPLRAGITWQFKEVGMLDEIPNNLIER 315

Query: 1445 MEENQAKTKRRKKDISNHRNKLSEIKISLIYMEWYMKSRKKYGGYYDSYKVQSTREEIVS 1624
            ME+ Q +  + KK       KL+++KISL +MEWYMK+ K   GYYD+YK   T +E  S
Sbjct: 316  MEDKQTRAIKSKKHTYEPTKKLNDMKISLTFMEWYMKTWKPKRGYYDTYKNPETIKEKDS 375

Query: 1625 HRVIYTHKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADH---- 1792
            +  I  H+L+LNQ+W+K VTE +L+P +E AK+RK WL S  NYRRIVEPLDIA+H    
Sbjct: 376  NNGIELHRLRLNQYWQKFVTEKDLLPQKEGAKLRKRWLYSGNNYRRIVEPLDIAEHYKSE 435

Query: 1793 ---YKKGNSNYTEQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEAL 1963
               YK GN +Y   RPNHYKLLE W NED++  +   G   KAA+LT+D+CFWAHVEEAL
Sbjct: 436  GKYYKNGNKHYLADRPNHYKLLEDWSNEDKKGSDNPIGGKKKAASLTEDSCFWAHVEEAL 495

Query: 1964 ILLNDLRNEGSSDNIDGILLGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYR 2143
            I L +L+NEGS  + D     LE FE  +M EI  Y +SPD+F++GSS M+WWNEYK ++
Sbjct: 496  ISLRELKNEGSGSHADVRNQELENFETKVMGEIKSYSLSPDVFLEGSSFMKWWNEYKQHK 555

Query: 2144 GSMYASEFAQYMNSKSYTSY 2203
            G  Y SEFA+YMN +SY  Y
Sbjct: 556  GGSYVSEFARYMNERSYKLY 575



 Score =  454 bits (1167), Expect = e-141
 Identities = 270/572 (47%), Positives = 351/572 (61%), Gaps = 18/572 (3%)
 Frame = +2

Query: 509  RETMNQHIAIAPKCYESEMFSSEVELSNLLGSFDILPDAYQAILKTSSA--YQLHTDIQS 682
            +E+ + H+A A       +F SEVEL   LGS D++ DAY A   TSS+  Y+LH +  S
Sbjct: 613  QESFSSHLAKA-------VFCSEVELGGFLGSLDLIEDAYHANFDTSSSTSYKLHHNF-S 664

Query: 683  GIQFLAFNISSEYTTRFLKGEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFE 862
            G Q L+F+ SS +TTRFL G F+LVSS++H V+DF+STK+ P+FSIN+AAV LFE +  E
Sbjct: 665  GFQVLSFHSSSHHTTRFLNGGFDLVSSKDHPVIDFVSTKLNPLFSINEAAVRLFEGLFLE 724

Query: 863  LSQIEYQLTYKPLVVTGRCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLL 1042
            L ++E Q T                                      KRPICITFG PL+
Sbjct: 725  LKELENQDT--------------------------------------KRPICITFGCPLV 746

Query: 1043 GNEALQHAICERPTWKSSFLNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDP 1222
            GN+ L+ AI ERP WKS FLNVV++ DPVA F+SS   YKPFGT LFC ESGG+ V ED 
Sbjct: 747  GNQDLRKAISERPQWKSRFLNVVAQTDPVARFYSSNNLYKPFGTSLFCTESGGHMVFEDQ 806

Query: 1223 DTILAVLDSMGSSIAENLEIHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLME 1402
            + + AVLD+M SSI  N EI  YGN L +I  K LYRGVSELGE  ++S RAGITLQ  E
Sbjct: 807  ELVPAVLDAMASSITGNTEI-DYGNHLKSIRRKVLYRGVSELGEFKLDSLRAGITLQFSE 865

Query: 1403 -AXXXXXXXXXXXXRMEENQAKTKRRKK-----DISNHRNKLSEIKISLIYMEWYMKSRK 1564
                          R+ E Q   KR+++     D  N + +  E++  ++ ME YMK  +
Sbjct: 866  VGHVLDDNLNDLVGRIAEEQKIAKRKEQLNFFYDRINSKKRNEEMR-GILCMELYMKKGR 924

Query: 1565 KYGGYYDSYK-VQSTREEIVSHRVIYTHKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLS 1741
              GG YDS+K    ++ EI  ++ I  +K  LNQ+W++ V ENN MP +E  K+ +  L 
Sbjct: 925  SKGGSYDSFKNPGESKLEIELYQEIVKNKRILNQYWKEKVDENNRMPKKEGTKLHRRLLY 984

Query: 1742 SATNYRRIVEPLDIADH-------YKKGNSNYTEQRPNHYKLLEKWLNED--QQDLNPSE 1894
                YRRIVEPLDIA+H       YK G  +Y E R  HY+LLE+WLNE   ++ LN SE
Sbjct: 985  RGNTYRRIVEPLDIAEHYKLNGEYYKNGEKHYLENRSQHYQLLEEWLNEHNYKKGLNQSE 1044

Query: 1895 GEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGILLGLEGFEAYLMREINGYL 2074
                KA+T+  D+CFWA+VEEA ILL+ L+N GS+D        LE FE Y+M EI  Y 
Sbjct: 1045 -RTNKASTINDDSCFWAYVEEASILLSKLKNGGSADVRS---QELEYFENYVMSEIKTYS 1100

Query: 2075 VSPDIFMDGSSLMRWWNEYKTYRGSMYASEFA 2170
            +SPD+F+ GSSLM+WWNEYK ++GS+YASEFA
Sbjct: 1101 LSPDVFIKGSSLMKWWNEYKQHKGSLYASEFA 1132


>ref|XP_023743631.1| senescence-associated carboxylesterase 101-like [Lactuca sativa]
          Length = 541

 Score =  556 bits (1434), Expect = 0.0
 Identities = 292/548 (53%), Positives = 377/548 (68%)
 Frame = +2

Query: 563  MFSSEVELSNLLGSFDILPDAYQAILKTSSAYQLHTDIQSGIQFLAFNISSEYTTRFLKG 742
            MF+S+VEL   +GS D++PDAY+AI +TSS  QLHT  +SGI+ LAF+   +YT RF   
Sbjct: 1    MFNSDVELGKFVGSTDLIPDAYRAITETSSTCQLHTT-RSGIKVLAFSSFPDYTFRFHNR 59

Query: 743  EFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTGRCL 922
            EF+LVSS +H + DFISTKV P FSIN A V+LFE    +L ++  QL   PLV+TG+ +
Sbjct: 60   EFDLVSSEHHEMFDFISTKVNPKFSINGAGVELFEQHFAKLLELLNQLIDSPLVITGQGI 119

Query: 923  GGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSSFL 1102
            GGYLAILF L  Q  +D++E NG  + KRPICITFG P+LGNE LQ AI ERP WKSSFL
Sbjct: 120  GGYLAILFALRHQHEVDVQESNGSKSAKRPICITFGCPMLGNEHLQGAIAERPQWKSSFL 179

Query: 1103 NVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAENLEI 1282
            NVV+K DP+ASFFSS +PYKPFGTFLFC ESGG++  ED D ILA LD+M SS A N ++
Sbjct: 180  NVVAKTDPLASFFSSNSPYKPFGTFLFCTESGGHAAFEDQDAILAALDAMVSSNAGNSQM 239

Query: 1283 HSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEENQA 1462
            H Y  EL +I  K LYRG SE  E ++   RAGI  Q  E              ME+ Q 
Sbjct: 240  HDYSKELGSIRKKVLYRGPSEFSESNVTPLRAGIVFQFQEIGVLNDISNDLITEMEKKQV 299

Query: 1463 KTKRRKKDISNHRNKLSEIKISLIYMEWYMKSRKKYGGYYDSYKVQSTREEIVSHRVIYT 1642
            K  + KK     + KL+++KI+L YMEWYMK+R+  GGYYD YK  S  +++ +  VI  
Sbjct: 300  KIIKSKKRYEPTK-KLNDMKINLTYMEWYMKTRRLKGGYYDIYKNVSDTDKVENKDVIIK 358

Query: 1643 HKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYTE 1822
             +  L+Q+W+KTV E++LMP ++ AK+RK WL + TNYRRI+EPLDIAD+YK G  NY  
Sbjct: 359  PQRFLDQYWKKTVDESDLMPQKQGAKLRKRWLYNGTNYRRIMEPLDIADYYKSGRKNYIA 418

Query: 1823 QRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSD 2002
             RP HY+LLEKW  +++++  PSE   +KAA+LT+D+CFWAHVEEA I L  L+N   S 
Sbjct: 419  NRPKHYELLEKWSEDEKKERKPSEVR-MKAASLTEDSCFWAHVEEAFISLRGLKNGDLSC 477

Query: 2003 NIDGILLGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYASEFAQYMN 2182
            N   I      FEAYL+  IN   +SPD+F++GSSLM+WW+EY  Y+GS    E A++MN
Sbjct: 478  NTTDI----GKFEAYLLGAINEKSLSPDVFVEGSSLMKWWSEYYAYKGSSCNPELARFMN 533

Query: 2183 SKSYTSYQ 2206
            + SY SY+
Sbjct: 534  NGSYKSYE 541


>ref|XP_023743645.1| senescence-associated carboxylesterase 101-like [Lactuca sativa]
 gb|PLY96561.1| hypothetical protein LSAT_4X181061 [Lactuca sativa]
          Length = 555

 Score =  547 bits (1409), Expect = 0.0
 Identities = 287/556 (51%), Positives = 372/556 (66%), Gaps = 5/556 (0%)
 Frame = +2

Query: 554  ESEMFSSEVELSNLLGSFDILPDAYQAILKTSSAYQLHTDIQSGIQFLAFNISSEYTTRF 733
            + +MFSSEVEL   LGS D++ DAY+AI +TSS + LH   +SG   LAFN  S+Y+   
Sbjct: 3    DQKMFSSEVELGKFLGSTDVIQDAYRAISQTSSTHHLHPT-RSGFNVLAFNSLSDYSILI 61

Query: 734  LKGEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTG 913
            +    +LV+  NH  + F+STKV P  SINKAA++LFE    EL ++  Q    PLVVTG
Sbjct: 62   INSALDLVTPENHKDLHFLSTKVNPECSINKAAIELFEQHFDELLELLKQHKDSPLVVTG 121

Query: 914  RCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKS 1093
              LGGYLAILF L  Q A+D++E  G    KRPICITFG+PLLG+E L+ AICERP W S
Sbjct: 122  HGLGGYLAILFALLHQHAVDVEESKGLKTAKRPICITFGAPLLGDETLRRAICERPQWSS 181

Query: 1094 SFLNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAEN 1273
             FLNVV+  D +AS FSS T YKPFGTFLFC ESGG++  ED +TILAVLD + SS   N
Sbjct: 182  CFLNVVANTDTLASVFSSKTLYKPFGTFLFCTESGGHTAFEDQETILAVLDDIASSNGGN 241

Query: 1274 LEIHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEE 1453
             + H Y NEL  I  K LYRG SE G  ++    AGI LQ  E             + +E
Sbjct: 242  YQTHDYTNELGLIRRKILYRGPSEFGGFNLTPLTAGIMLQFQEIGLLKDDSQDLIAKTKE 301

Query: 1454 NQAKTKRRKKDISNHR--NKLSEIKISLIYMEWYMKSRKKYGGYYDSYKVQSTR--EEIV 1621
             +AK  + K+ ++ +    KL+E+KISL YMEWYMK ++  GGYYDS+K   T+  E+I 
Sbjct: 302  KRAKMIKTKRMVNAYEPTKKLNEMKISLTYMEWYMKGQRSRGGYYDSFKNAETKTIEDIR 361

Query: 1622 SHRVIYTHKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKK 1801
            S + I  ++  L+Q+W+KT+ E  LMP +E AK+RK WL   TNYRRIVEPLDIAD+YKK
Sbjct: 362  SQQEIIRNQRILSQYWKKTIEEKELMPQKEGAKLRKRWLYGGTNYRRIVEPLDIADYYKK 421

Query: 1802 GNSNYTEQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDL 1981
            GN+NY E RPNHY LLE+W  +D++D  PS  +  KAA+LT+D+CFWAHVEEALI L DL
Sbjct: 422  GNTNYIESRPNHYVLLERWSEDDKKDQKPS--DKTKAASLTEDSCFWAHVEEALISLRDL 479

Query: 1982 RNEGSSDNIDGI-LLGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYA 2158
            +N G  D    +    L+ FE  ++  I  + +SP++F+ GSSLM+WW +YK ++G  Y 
Sbjct: 480  KNGGLDDITTSLKTSSLKRFEEDILCAIKAFSLSPEVFVPGSSLMKWWIDYKVFKGIGYH 539

Query: 2159 SEFAQYMNSKSYTSYQ 2206
            SEFA+YMN++ YT YQ
Sbjct: 540  SEFAEYMNNERYTEYQ 555


>ref|XP_021984326.1| senescence-associated carboxylesterase 101-like [Helianthus annuus]
 gb|OTG16751.1| putative senescence associated protein [Helianthus annuus]
          Length = 545

 Score =  522 bits (1344), Expect = e-174
 Identities = 276/551 (50%), Positives = 361/551 (65%), Gaps = 3/551 (0%)
 Frame = +2

Query: 563  MFSSEVELSNLLGSFDILPDAYQAILKTS---SAYQLHTDIQSGIQFLAFNISSEYTTRF 733
            MF SEVEL   L S DI+P  +  + K +   S Y+LHT    GI+ LAFN S +YTTRF
Sbjct: 5    MFCSEVELGKFLSSIDIIPYVFNVVRKLAEKNSPYELHTS-PYGIKVLAFNYSLDYTTRF 63

Query: 734  LKGEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTG 913
              GEF+LVSS++H VVDFISTKV P F INKAAV+LF+++   L +++ +    PLVVTG
Sbjct: 64   ANGEFDLVSSQSHEVVDFISTKVNPSFFINKAAVELFKHVKNNLKELKKEDLKNPLVVTG 123

Query: 914  RCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKS 1093
              LGGYLAI+ TLWLQ  +  +E       KRPICITFG+PL+G+EAL+ AI E P W+S
Sbjct: 124  WGLGGYLAIMTTLWLQSTMYEEE------TKRPICITFGTPLIGDEALRLAISESPQWES 177

Query: 1094 SFLNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAEN 1273
             FLNVVS  D +ASF  S   Y+PFG+FLFC E GG++  +D + ILAVLD++      N
Sbjct: 178  CFLNVVSMTDSIASFVPSKGKYEPFGSFLFCTELGGHATFKDHEAILAVLDALALPRDAN 237

Query: 1274 LEIHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEE 1453
            L+ H+Y  +L++I  + L RGV ELGE  +     GI +Q  E             +ME 
Sbjct: 238  LKNHNYEIDLSSIRKRVLRRGVFELGEYKLKPMTKGINMQFKEIGVLDKISNDMIVKMEL 297

Query: 1454 NQAKTKRRKKDISNHRNKLSEIKISLIYMEWYMKSRKKYGGYYDSYKVQSTREEIVSHRV 1633
             Q K  + KK+      KL++IKIS+ ++EWY+K+R+  GGYYD YK + TR+E   H+ 
Sbjct: 298  KQEKMIKSKKNTYEPTKKLNDIKISMTFIEWYIKTRQLKGGYYDCYKKEPTRDEKEGHQK 357

Query: 1634 IYTHKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSN 1813
            I    L LN+FW K V E +  P +E  K+RK WL S  NYRR++EPLDIA HY  G ++
Sbjct: 358  IIKEHLNLNKFWEKFVMEKDQNPQKEGGKLRKRWLYSGNNYRRMIEPLDIAKHYANGKTD 417

Query: 1814 YTEQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEG 1993
            Y   R NHYKLLE W  ED++D++PS G   KA  L +D+CFWA+VEEALI LN+L+  G
Sbjct: 418  YLAIRSNHYKLLETWSKEDKKDVDPSAGRN-KAPNLNEDSCFWAYVEEALISLNELKMSG 476

Query: 1994 SSDNIDGILLGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYASEFAQ 2173
            +  N D I   L+ F  Y+MR +N Y VSPDIF++GSSLM+WW+EY+ Y   +Y SEFAQ
Sbjct: 477  A--NGDDIAWKLKPFMDYVMRGVNDYSVSPDIFLEGSSLMKWWSEYEAYAQGLYTSEFAQ 534

Query: 2174 YMNSKSYTSYQ 2206
            YMN+  Y SYQ
Sbjct: 535  YMNNGRYASYQ 545


>ref|XP_023743620.1| senescence-associated carboxylesterase 101-like [Lactuca sativa]
 gb|PLY96582.1| hypothetical protein LSAT_4X180961 [Lactuca sativa]
          Length = 533

 Score =  501 bits (1289), Expect = e-166
 Identities = 283/550 (51%), Positives = 358/550 (65%), Gaps = 1/550 (0%)
 Frame = +2

Query: 560  EMFSSEVELSNLLGSFDILPDAYQAILKTSSAYQLHTDIQSGIQFLAFNISSEYTTRFLK 739
            +MFSSEVE    L S D++ DA+ A  KTSS YQLHT+  SGI+ LAFN  S+Y  RF  
Sbjct: 4    KMFSSEVEGGKFLVSIDVIQDAFGATAKTSSTYQLHTN-PSGIKVLAFNCLSDYKIRFRN 62

Query: 740  GEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTGRC 919
            GE +LVSS  H VVDFISTK+ P  SINKAAV+LFE    ELS++  QL  +PLVVTG  
Sbjct: 63   GE-DLVSSDKHKVVDFISTKLNPKLSINKAAVELFELHFKELSELSNQLINEPLVVTGCG 121

Query: 920  LGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSSF 1099
            LGGYL+IL+TL  Q   D++E  G    KRPICITFGSPLLGN+ LQ AI ERP WKSSF
Sbjct: 122  LGGYLSILYTLLHQHYADVEESKGSKTTKRPICITFGSPLLGNQHLQGAISERPQWKSSF 181

Query: 1100 LNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAENLE 1279
            LNVV+K D +ASFFSS + YKPFGTFLFC ESGG++  ED D ILAVLD+M S  A N +
Sbjct: 182  LNVVAKTDFLASFFSSNSQYKPFGTFLFCTESGGHTAFEDQDAILAVLDAMVSPNAGNDQ 241

Query: 1280 IHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEENQ 1459
             H Y  EL +I  K LYRG S+  E  ++  RAGI  Q  E             ++E+ Q
Sbjct: 242  THDYSKELVSIRKKILYRGPSDFSESHLSPLRAGILFQFQEIGMLHNISNDFIDKIEKKQ 301

Query: 1460 AKTKRRKKDISNHRNKLSEIKISLIYMEWYMKSRKKYGGYYDSYKVQSTREEIVSHRVIY 1639
             K   + K++     KL++ KI+L  MEWYMK+R+  GGYYD+YK     ++  S  VI 
Sbjct: 302  VK-MIKMKNVYEPARKLNDTKINLTLMEWYMKTRRSKGGYYDAYKNADITDKFESKDVII 360

Query: 1640 THKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYT 1819
              +    Q+W++TV  ++LM        R  WL + TNYRRI+EPLDIAD+YK G  NY 
Sbjct: 361  KLQRFQEQYWKETVKNSDLMS-------RMRWLYNGTNYRRIMEPLDIADYYKSGRKNYI 413

Query: 1820 EQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRN-EGS 1996
              RP H++LL+K L+ED++ +        KAA+LT D+CFWAHVEEALI L DL+N + S
Sbjct: 414  ANRPEHFELLQK-LSEDEKKVR------TKAASLTVDSCFWAHVEEALISLTDLKNGDLS 466

Query: 1997 SDNIDGILLGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYASEFAQY 2176
            S   D        FE YL+  IN   +SPD+F++GSSLM+WW+EY  Y+GS   +E A+Y
Sbjct: 467  STTTD---TDQFDFEVYLLGAINEKSLSPDVFLEGSSLMKWWSEYDAYKGSSGNAELARY 523

Query: 2177 MNSKSYTSYQ 2206
            M ++ Y SYQ
Sbjct: 524  MKNERYKSYQ 533


>gb|PLY96564.1| hypothetical protein LSAT_4X180981 [Lactuca sativa]
          Length = 471

 Score =  486 bits (1251), Expect = e-161
 Identities = 253/475 (53%), Positives = 324/475 (68%)
 Frame = +2

Query: 782  DFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTGRCLGGYLAILFTLWLQ 961
            DFISTKV P FSIN A V+LFE    +L ++  QL   PLV+TG+ +GGYLAILF L  Q
Sbjct: 3    DFISTKVNPKFSINGAGVELFEQHFAKLLELLNQLIDSPLVITGQGIGGYLAILFALRHQ 62

Query: 962  QAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSSFLNVVSKKDPVASFF 1141
              +D++E NG  + KRPICITFG P+LGNE LQ AI ERP WKSSFLNVV+K DP+ASFF
Sbjct: 63   HEVDVQESNGSKSAKRPICITFGCPMLGNEHLQGAIAERPQWKSSFLNVVAKTDPLASFF 122

Query: 1142 SSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAENLEIHSYGNELNAIGSK 1321
            SS +PYKPFGTFLFC ESGG++  ED D ILA LD+M SS A N ++H Y  EL +I  K
Sbjct: 123  SSNSPYKPFGTFLFCTESGGHAAFEDQDAILAALDAMVSSNAGNSQMHDYSKELGSIRKK 182

Query: 1322 GLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEENQAKTKRRKKDISNHR 1501
             LYRG SE  E ++   RAGI  Q  E              ME+ Q K  + KK     +
Sbjct: 183  VLYRGPSEFSESNVTPLRAGIVFQFQEIGVLNDISNDLITEMEKKQVKIIKSKKRYEPTK 242

Query: 1502 NKLSEIKISLIYMEWYMKSRKKYGGYYDSYKVQSTREEIVSHRVIYTHKLKLNQFWRKTV 1681
             KL+++KI+L YMEWYMK+R+  GGYYD YK  S  +++ +  VI   +  L+Q+W+KTV
Sbjct: 243  -KLNDMKINLTYMEWYMKTRRLKGGYYDIYKNVSDTDKVENKDVIIKPQRFLDQYWKKTV 301

Query: 1682 TENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYTEQRPNHYKLLEKWL 1861
             E++LMP ++ AK+RK WL + TNYRRI+EPLDIAD+YK G  NY   RP HY+LLEKW 
Sbjct: 302  DESDLMPQKQGAKLRKRWLYNGTNYRRIMEPLDIADYYKSGRKNYIANRPKHYELLEKWS 361

Query: 1862 NEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGILLGLEGFE 2041
             +++++  PSE   +KAA+LT+D+CFWAHVEEA I L  L+N   S N   I      FE
Sbjct: 362  EDEKKERKPSEVR-MKAASLTEDSCFWAHVEEAFISLRGLKNGDLSCNTTDI----GKFE 416

Query: 2042 AYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYASEFAQYMNSKSYTSYQ 2206
            AYL+  IN   +SPD+F++GSSLM+WW+EY  Y+GS    E A++MN+ SY SY+
Sbjct: 417  AYLLGAINEKSLSPDVFVEGSSLMKWWSEYYAYKGSSCNPELARFMNNGSYKSYE 471


>ref|XP_022029822.1| senescence-associated carboxylesterase 101-like [Helianthus annuus]
 gb|OTG32731.1| putative alpha/Beta hydrolase fold protein [Helianthus annuus]
          Length = 537

 Score =  481 bits (1238), Expect = e-158
 Identities = 269/553 (48%), Positives = 354/553 (64%), Gaps = 5/553 (0%)
 Frame = +2

Query: 563  MFSSEVELSNLLGSFDILPDAYQAILKTSSAYQLHTDIQSGIQFLAFNISSEYTTRFLKG 742
            MF  EVEL   LGS DI+P A+ AIL+T S Y+L     SGIQ LAFN SS+YTTRFL  
Sbjct: 5    MFGCEVELGKFLGSIDIIPYAFCAILETCSTYELRKS-PSGIQVLAFNCSSDYTTRFLDN 63

Query: 743  EFNLVSSRNHNVVDFIST-KVKPIFSINKAAVDLFENISFELSQIEYQL-TYKPLVVTGR 916
            EF LVSS  H  +DFIS  +V P FSINKAAV+LFEN++ +L  ++Y+L   +PLVVTGR
Sbjct: 64   EFGLVSSEEHKALDFISAGEVNPSFSINKAAVELFENLTNDLGHLKYKLEAERPLVVTGR 123

Query: 917  CLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSS 1096
             LGGY+AILF LWL QA+D+KE +   ++KRP+CITFG PL+G+E L   I + P W+S 
Sbjct: 124  GLGGYVAILFALWLHQAVDVKESDDDWSSKRPVCITFGCPLVGDENLGRIISKYPQWESR 183

Query: 1097 FLNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAENL 1276
            FLNVV+K D VAS FSS + Y+PFGTFLFC ESG  +  +D D ILAVLD MG    EN 
Sbjct: 184  FLNVVAKDDRVASVFSSNSRYRPFGTFLFCTESGARTAFQDQDLILAVLDKMGLPRTENT 243

Query: 1277 EIHSYGNELNAIGSKGLYRGVSELGELSMNSFRAGITLQLMEAXXXXXXXXXXXXRMEEN 1456
            E   Y     AI  K L+RGVSE GE +   +   +                    +EE 
Sbjct: 244  ETPDYVKLFGAIRRKTLHRGVSESGEFNKPEYFRDL---------------IDIGGVEEK 288

Query: 1457 QAKTKRRKKDISNHRNKLSEIKISLIYMEWYMKSRKKYGGYYDSY-KVQSTREEIVSHRV 1633
            +   ++  + I +   K++++KI ++ +E YM   +  GG YD Y K   ++EEI     
Sbjct: 289  RKMMRKGNRKIYDQTKKVNDMKIKMVNIEMYMNMTRSNGGIYDFYKKPNKSKEEIHIENK 348

Query: 1634 IYTHKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKK-GNS 1810
            I +H+  L + W+  V E    P +E AK+RK WL +  NYRR++EPL IA++YK  GN 
Sbjct: 349  ITSHQRALTKHWQDFVEETKQRPQREDAKMRKRWLYNGHNYRRMIEPLSIAEYYKTVGNR 408

Query: 1811 NYTEQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNE 1990
            +Y  QRP HY  LEK L   +  ++PS G   KAA+LT+D+CFWA+VEE++ILL  L+ E
Sbjct: 409  DYIGQRPPHYLELEKLL---KPYIDPSNGNK-KAASLTQDSCFWAYVEESIILLTYLKKE 464

Query: 1991 GSSDNIDGILLG-LEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRGSMYASEF 2167
              S N+   + G L+ FE Y+MR IN Y VSPDIF+ GSS M+WW+EYK  +GS Y SE 
Sbjct: 465  DLSHNLMNNIKGKLDLFEEYVMRSINDYSVSPDIFLTGSSFMKWWHEYKACKGSSYVSEL 524

Query: 2168 AQYMNSKSYTSYQ 2206
            A+YMN+ SY+ Y+
Sbjct: 525  ARYMNNGSYSLYK 537


>gb|OTF95917.1| putative alpha/Beta hydrolase fold protein [Helianthus annuus]
          Length = 546

 Score =  446 bits (1146), Expect = e-144
 Identities = 261/560 (46%), Positives = 333/560 (59%), Gaps = 13/560 (2%)
 Frame = +2

Query: 566  FSSEVELSNLLGSF-DIL-PDAYQAILKTSSAYQLHTDIQSGIQFLAFNISSE-YTTRFL 736
            F SE+EL   L +  D+L P A+  + + S  Y ++T   SGIQ L    S + YTTRFL
Sbjct: 5    FRSELELGYFLVTILDVLIPYAFGTVAENSLTYNIYTS-PSGIQVLDIQCSLDHYTTRFL 63

Query: 737  KGEFNLVSSRNHNVVDFISTKVKPIFSINKAAVDLFENISFELSQIEYQLTYKPLVVTGR 916
              EF+LVSS+NH VVDFI TK  P F  +      FE I  E+         + LVVTG 
Sbjct: 64   NKEFDLVSSKNHKVVDFILTKDNPHFHND------FEKIQNEVKD-------RKLVVTGI 110

Query: 917  CLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSS 1096
             LGGY+AIL+TLWL   ID K         RPICITFGSPLLG++AL+ AI ERP WKSS
Sbjct: 111  ALGGYIAILYTLWLHHTIDEKRKPDSKITTRPICITFGSPLLGDKALESAISERPEWKSS 170

Query: 1097 FLNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGGNSVLEDPDTILAVLDSM-----GSS 1261
            FLNVVS  DP A FFSS T YKP GTF+FC +S G++  ED D ILA+LD M     GS+
Sbjct: 171  FLNVVSTSDPFACFFSSNTRYKPIGTFVFCTQSSGHTTFEDNDAILAILDKMASSRSGSN 230

Query: 1262 IAENLEIHSYGNELNAIGSKGLYRGVSEL-GELSMNSFRAGITLQLMEAXXXXXXXXXXX 1438
               + ++H Y N+L +I S  LYRG  E+ GEL  N+ R GITLQ  E            
Sbjct: 231  PGISRQMHDYENDLRSIRSNVLYRGACEVAGELDSNALREGITLQFREIGALDISNDLIE 290

Query: 1439 XRMEENQAKTKRRKKDISNHRNKLSEIKISLIYMEWYMKSRKKYGGYYDSYKV-QSTREE 1615
              ME    K  R++ +       L++ KI +  ME ++ SR+   GYYD +K    T +E
Sbjct: 291  KLMEGEHEK-MRKRMNTKRLEGNLNKRKIKMAKMELFISSRRSRRGYYDRFKSGPMTIDE 349

Query: 1616 IVSHRVIYTHKLKLNQFWRKTVTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHY 1795
            +  H  I      LNQ+W + V E  LMP +E   +RK WL S  NYRR+ EPL IA++Y
Sbjct: 350  LSGHNEIINSHESLNQYWDEFVKEKQLMPQKEGVSLRKRWLYSGNNYRRMFEPLYIAEYY 409

Query: 1796 KKGNSNYTEQRPNHYKLLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLN 1975
            KKGN +Y E RPN Y+LLEKW NED+++LNP+E +  K   +  D+CFWA VEEALI L 
Sbjct: 410  KKGNISYIENRPNRYRLLEKWWNEDKKNLNPNERKE-KGPNVNDDSCFWARVEEALISLR 468

Query: 1976 DLRNEGSSDNIDGIL---LGLEGFEAYLMREINGYLVSPDIFMDGSSLMRWWNEYKTYRG 2146
             L N  SS N  G+      L+ F  Y+M  +N Y +S D F++GSSLM+WW EY  Y+ 
Sbjct: 469  ILTNGSSSSN--GVCEKEQKLDNFMTYMMHSVNDYSLSSDTFLEGSSLMQWWREYNAYKK 526

Query: 2147 SMYASEFAQYMNSKSYTSYQ 2206
             +  S+FA+YM S  Y SYQ
Sbjct: 527  GLCTSKFAEYMKSGRYKSYQ 546


>ref|XP_022014633.1| senescence-associated carboxylesterase 101-like [Helianthus annuus]
          Length = 462

 Score =  395 bits (1015), Expect = e-126
 Identities = 219/465 (47%), Positives = 281/465 (60%), Gaps = 15/465 (3%)
 Frame = +2

Query: 857  FELSQIEYQLTY-----KPLVVTGRCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICI 1021
            +++S   Y  TY     + LVVTG  LGGY+AIL+TLWL   ID K         RPICI
Sbjct: 2    YQVSLFYYFNTYLQVKDRKLVVTGIALGGYIAILYTLWLHHTIDEKRKPDSKITTRPICI 61

Query: 1022 TFGSPLLGNEALQHAICERPTWKSSFLNVVSKKDPVASFFSSYTPYKPFGTFLFCEESGG 1201
            TFGSPLLG++AL+ AI ERP WKSSFLNVVS  DP A FFSS T YKP GTF+FC +S G
Sbjct: 62   TFGSPLLGDKALESAISERPEWKSSFLNVVSTSDPFACFFSSNTRYKPIGTFVFCTQSSG 121

Query: 1202 NSVLEDPDTILAVLDSM-----GSSIAENLEIHSYGNELNAIGSKGLYRGVSEL-GELSM 1363
            ++  ED D ILA+LD M     GS+   + ++H Y N+L +I S  LYRG  E+ GEL  
Sbjct: 122  HTTFEDNDAILAILDKMASSRSGSNPGISRQMHDYENDLRSIRSNVLYRGACEVAGELDS 181

Query: 1364 NSFRAGITLQLMEAXXXXXXXXXXXXRMEENQAKTKRRKKDISNHRNKLSEIKISLIYME 1543
            N+ R GITLQ  E              ME    K  R++ +       L++ KI +  ME
Sbjct: 182  NALREGITLQFREIGALDISNDLIEKLMEGEHEK-MRKRMNTKRLEGNLNKRKIKMAKME 240

Query: 1544 WYMKSRKKYGGYYDSYKV-QSTREEIVSHRVIYTHKLKLNQFWRKTVTENNLMPPQEAAK 1720
             ++ SR+   GYYD +K    T +E+  H  I      LNQ+W + V E  LMP +E   
Sbjct: 241  LFISSRRSRRGYYDRFKSGPMTIDELSGHNEIINSHESLNQYWDEFVKEKQLMPQKEGVS 300

Query: 1721 VRKGWLSSATNYRRIVEPLDIADHYKKGNSNYTEQRPNHYKLLEKWLNEDQQDLNPSEGE 1900
            +RK WL S  NYRR+ EPL IA++YKKGN +Y E RPN Y+LLEKW NED+++LNP+E +
Sbjct: 301  LRKRWLYSGNNYRRMFEPLYIAEYYKKGNISYIENRPNRYRLLEKWWNEDKKNLNPNERK 360

Query: 1901 YIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGIL---LGLEGFEAYLMREINGY 2071
              K   +  D+CFWA VEEALI L  L N  SS N  G+      L+ F  Y+M  +N Y
Sbjct: 361  E-KGPNVNDDSCFWARVEEALISLRILTNGSSSSN--GVCEKEQKLDNFMTYMMHSVNDY 417

Query: 2072 LVSPDIFMDGSSLMRWWNEYKTYRGSMYASEFAQYMNSKSYTSYQ 2206
             +S D F++GSSLM+WW EY  Y+  +  S+FA+YM S  Y SYQ
Sbjct: 418  SLSSDTFLEGSSLMQWWREYNAYKKGLCTSKFAEYMKSGRYKSYQ 462


>gb|KVH92759.1| Lipase, class 3, partial [Cynara cardunculus var. scolymus]
          Length = 374

 Score =  345 bits (884), Expect = e-108
 Identities = 192/372 (51%), Positives = 246/372 (66%), Gaps = 11/372 (2%)
 Frame = +2

Query: 977  KELNGYTNNKRPICITFGSPLLGNEALQHAICERPTWKSSFLNVVSKKDPVASFFSSYTP 1156
            KE +G+ + KRPICITFG PL+GN+ L+ AI ERP WKS FLNVV++ DPVA F+SS + 
Sbjct: 2    KEDDGFKDTKRPICITFGCPLVGNQDLRKAISERPQWKSRFLNVVAQTDPVARFYSSNSL 61

Query: 1157 YKPFGTFLFCEESGGNSVLEDPDTILAVLDSMGSSIAENLEIHSYGNELNAIGSKGLYRG 1336
            YKPFGT LFC ESGG+ V ED +++ A LD+M SSI EN EI  YGN L +I  K LYRG
Sbjct: 62   YKPFGTSLFCTESGGHMVFEDQESVPAALDAMASSITENTEI-DYGNHLKSIRRKVLYRG 120

Query: 1337 VSELGELSMNSFRAGITLQLME-AXXXXXXXXXXXXRMEENQ--AKTKRRKKDISNHRNK 1507
            VS+LGE  ++S RAGITLQ  E              RMEE Q  AK K   +       K
Sbjct: 121  VSKLGEFGLDSLRAGITLQFSEVGHVLDDNCNDLIGRMEEEQRLAKRKSNLRYDRTKSKK 180

Query: 1508 LSEIKISLIYMEWYMKSRKKYGGYYDSYKVQ-STREEIVSHRVIYTHKLKLNQFWRKTVT 1684
            L++ K S++ ME YMK+ +  GGYYD +K    ++ EI S++ I  +K  L+Q+W++TV 
Sbjct: 181  LNKAKTSMVCMELYMKNERSKGGYYDRFKTPVKSKSEIESYQEIVKNKRILDQYWKETVA 240

Query: 1685 ENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADH-------YKKGNSNYTEQRPNHYK 1843
            ENNLMP  + AK+R   L     YR+IVEPLDIA+H       YK G  +Y E R  HY+
Sbjct: 241  ENNLMPQNKGAKLRNCLLYGGNTYRKIVEPLDIAEHYKLNGEYYKNGEKHYLEIRSQHYQ 300

Query: 1844 LLEKWLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGILL 2023
            LLEKWLNED++ LNPSE    KAA+L  D+CFWAHVEEA +LL++L+N GS+D + G   
Sbjct: 301  LLEKWLNEDKKGLNPSERSN-KAASLNDDSCFWAHVEEASVLLSELKNGGSTD-VRG--Q 356

Query: 2024 GLEGFEAYLMRE 2059
             LE FE Y+M +
Sbjct: 357  KLEQFETYVMTQ 368


>ref|XP_016510829.1| PREDICTED: senescence-associated carboxylesterase 101-like isoform X2
            [Nicotiana tabacum]
          Length = 582

 Score =  347 bits (889), Expect = e-106
 Identities = 225/597 (37%), Positives = 331/597 (55%), Gaps = 47/597 (7%)
 Frame = +2

Query: 554  ESEMFSSEVELSNLLGSFDILPDAYQAILKTSS-------------AYQLHTDIQSGIQF 694
            +  +FSS  EL+NL+ S D+L D++  I +  S             A++++    +    
Sbjct: 3    QDSLFSSGQELANLVVSSDLLHDSWATIYELLSHAYLNNPTNLAPIAFKVYYPYNTNGAI 62

Query: 695  LAFNISSEYTTRFLKGEFNLVSSRNHNV----VDFISTKVKPIFSINKAAVDLFENISFE 862
            +AF +SS   T     +  +VSS +        DFISTK+ P FS++K A+ LF ++  +
Sbjct: 63   IAF-VSSPTCTTVHHLQKEMVSSEDLKGSQLDFDFISTKLNPHFSVHKGAIALFASLLNQ 121

Query: 863  LSQIEYQL-TYKPLVVTGRCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPL 1039
            LS ++ QL ++ PL++TG  LGG +A LFTLWL   I +K+     NNKRP CITFGSP 
Sbjct: 122  LSALKLQLDSFSPLIITGHSLGGSVASLFTLWLLDNISLKD-----NNKRPTCITFGSPF 176

Query: 1040 LGNEALQHAICERPTWKSSFLNVVSKKDPVASFFSSYT-----------PYKPFGTFLFC 1186
            LG+  LQ AI ERP+W SSFL+V S +DP+  FF S T            YKPFGTFL C
Sbjct: 177  LGDRGLQQAISERPSWNSSFLHVASNQDPIPRFFISPTNGFSGSIPQSCVYKPFGTFLLC 236

Query: 1187 EESGGNSVLEDPDTILAVLDSMGSSIAENLE----IHSYGNELNAIGSKGLYRGVSELGE 1354
             +S   S  E+P+++L ++ +M +SI++  +    + +YG  L  +  + + +G S+L +
Sbjct: 237  SDS-DCSCFEEPESVLELMMAM-NSISQQQDNGFLVFNYGPILECLKHRVICKGTSQLSD 294

Query: 1355 LSMNSFRAGITLQL--MEAXXXXXXXXXXXXRMEENQAKTKRRKKDISNHRNKLSEIKIS 1528
              ++  +AGI LQL  +               MEE    +  +K +  +   KLS+IK  
Sbjct: 295  FGVSQLQAGIILQLEAIGIGGQQTSNMSFLRSMEERVKASFVKKTNAFDPGKKLSKIKED 354

Query: 1529 LIYMEWYMKSRKKYGGYYDSYK--------VQSTREEIVS-HRVIYTHKLKLNQFWRKTV 1681
            + Y+EWY K+    GGYY  +K        V  +REEIV  HR+       L ++W++ V
Sbjct: 355  MAYLEWYKKATLNEGGYYICFKERGSGSRDVVKSREEIVKRHRI-------LTKYWKRMV 407

Query: 1682 TENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYTE-QRPNHYKLLEKW 1858
             E   +P +E A  R  WL   TNYRR+VEPL+IA++Y KGN +Y    R  HYKLLEKW
Sbjct: 408  AEVEKLPQKEEAAFRTRWLYGGTNYRRMVEPLEIAEYYMKGNRDYVNFGRSEHYKLLEKW 467

Query: 1859 LNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGILLGLEGF 2038
            +N+D+  +     +  KA +LT+D+CFWA+VEEA+I    LR EGSS+  +     L  F
Sbjct: 468  MNKDK--IGGIANDRRKAVSLTEDSCFWAYVEEAIITSKGLR-EGSSEEKEKSRRNLVNF 524

Query: 2039 EAYLMREINGYLVSPDIFMDGSSLM-RWWNEYK-TYRGSMYASEFAQYMNSKSYTSY 2203
              Y+M  I  Y VS +IF   S+ M +WW EY+     S+Y+     YM ++ Y SY
Sbjct: 525  GEYVMSLIRSYSVSTEIFQPQSTFMKKWWQEYRQDILSSLYSCPLTFYMENEEYRSY 581


>ref|XP_016510827.1| PREDICTED: senescence-associated carboxylesterase 101-like isoform X1
            [Nicotiana tabacum]
 ref|XP_016510828.1| PREDICTED: senescence-associated carboxylesterase 101-like isoform X1
            [Nicotiana tabacum]
          Length = 583

 Score =  346 bits (887), Expect = e-106
 Identities = 225/598 (37%), Positives = 330/598 (55%), Gaps = 48/598 (8%)
 Frame = +2

Query: 554  ESEMFSSEVELSNLLGSFDILPDAYQAILKTSS-------------AYQLHTDIQSGIQF 694
            +  +FSS  EL+NL+ S D+L D++  I +  S             A++++    +    
Sbjct: 3    QDSLFSSGQELANLVVSSDLLHDSWATIYELLSHAYLNNPTNLAPIAFKVYYPYNTNGAI 62

Query: 695  LAFNISSEYTTRFLKGEFNLVSSRNHNV----VDFISTKVKPIFSINKAAVDLFENISFE 862
            +AF +SS   T     +  +VSS +        DFISTK+ P FS++K A+ LF ++  +
Sbjct: 63   IAF-VSSPTCTTVHHLQKEMVSSEDLKGSQLDFDFISTKLNPHFSVHKGAIALFASLLNQ 121

Query: 863  LSQIEYQL-TYKPLVVTGRCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPL 1039
            LS ++ QL ++ PL++TG  LGG +A LFTLWL   I +K+     NNKRP CITFGSP 
Sbjct: 122  LSALKLQLDSFSPLIITGHSLGGSVASLFTLWLLDNISLKD-----NNKRPTCITFGSPF 176

Query: 1040 LGNEALQHAICERPTWKSSFLNVVSKKDPVASFFSSYT-----------PYKPFGTFLFC 1186
            LG+  LQ AI ERP+W SSFL+V S +DP+  FF S T            YKPFGTFL C
Sbjct: 177  LGDRGLQQAISERPSWNSSFLHVASNQDPIPRFFISPTNGFSGSIPQSCVYKPFGTFLLC 236

Query: 1187 EESGGNSVLEDPDTILAVLDSMGSSIAENLE----IHSYGNELNAIGSKGLYRGVSELGE 1354
             +S   S  E+P+++L ++ +M +SI++  +    + +YG  L  +  + + +G S+L +
Sbjct: 237  SDS-DCSCFEEPESVLELMMAM-NSISQQQDNGFLVFNYGPILECLKHRVICKGTSQLSD 294

Query: 1355 LSMNSFRAGITLQLME---AXXXXXXXXXXXXRMEENQAKTKRRKKDISNHRNKLSEIKI 1525
              ++  +AGI LQL                   MEE    +  +K +  +   KLS+IK 
Sbjct: 295  FGVSQLQAGIILQLEAIGIGGQQQTSNMSFLRSMEERVKASFVKKTNAFDPGKKLSKIKE 354

Query: 1526 SLIYMEWYMKSRKKYGGYYDSYK--------VQSTREEIVS-HRVIYTHKLKLNQFWRKT 1678
             + Y+EWY K+    GGYY  +K        V  +REEIV  HR+       L ++W++ 
Sbjct: 355  DMAYLEWYKKATLNEGGYYICFKERGSGSRDVVKSREEIVKRHRI-------LTKYWKRM 407

Query: 1679 VTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYTE-QRPNHYKLLEK 1855
            V E   +P +E A  R  WL   TNYRR+VEPL+IA++Y KGN +Y    R  HYKLLEK
Sbjct: 408  VAEVEKLPQKEEAAFRTRWLYGGTNYRRMVEPLEIAEYYMKGNRDYVNFGRSEHYKLLEK 467

Query: 1856 WLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGILLGLEG 2035
            W+N+D+  +     +  KA +LT+D+CFWA+VEEA+I    LR EGSS+  +     L  
Sbjct: 468  WMNKDK--IGGIANDRRKAVSLTEDSCFWAYVEEAIITSKGLR-EGSSEEKEKSRRNLVN 524

Query: 2036 FEAYLMREINGYLVSPDIFMDGSSLM-RWWNEYK-TYRGSMYASEFAQYMNSKSYTSY 2203
            F  Y+M  I  Y VS +IF   S+ M +WW EY+     S+Y+     YM ++ Y SY
Sbjct: 525  FGEYVMSLIRSYSVSTEIFQPQSTFMKKWWQEYRQDILSSLYSCPLTFYMENEEYRSY 582


>ref|XP_019223911.1| PREDICTED: senescence-associated carboxylesterase 101-like isoform X2
            [Nicotiana attenuata]
          Length = 582

 Score =  343 bits (880), Expect = e-105
 Identities = 225/596 (37%), Positives = 324/596 (54%), Gaps = 46/596 (7%)
 Frame = +2

Query: 554  ESEMFSSEVELSNLLGSFDILPDAYQAILKTSS-------------AYQLHTDIQSGIQF 694
            +  +FSS  EL+NL+   D+L D++  I    S             A++++    +    
Sbjct: 3    QDSLFSSGQELANLVVISDLLHDSWATIYDLISHAYLNNPTNPAPIAFKVYYPYNTNGAI 62

Query: 695  LAFNISSEYTTRFLKGEFNLVSSRNHNV----VDFISTKVKPIFSINKAAVDLFENISFE 862
            +AF +SS   T     +  +VSS +        DFISTK+ P FS++K A+ LF ++  +
Sbjct: 63   VAF-VSSPTCTTLHHLQKEMVSSEDLEGSQLDFDFISTKLNPHFSLHKEAIALFASLLNQ 121

Query: 863  LSQIEYQL-TYKPLVVTGRCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPL 1039
            LS ++ QL ++ PL++TG  LGG +A LFTLWL   I  K+     NNKRP CITFGSP 
Sbjct: 122  LSALKLQLGSFSPLIITGHSLGGSVASLFTLWLLDNISPKD-----NNKRPTCITFGSPF 176

Query: 1040 LGNEALQHAICERPTWKSSFLNVVSKKDPVASF-------FSSYTP----YKPFGTFLFC 1186
            LG+   Q AI ERP+W SSFL+V S +DP+  F       FS   P    YKPFGTFL C
Sbjct: 177  LGDRGFQQAISERPSWNSSFLHVASNQDPIPRFLISPTNGFSGSIPQSCVYKPFGTFLLC 236

Query: 1187 EESGGNSVLEDPDTILAVLDSMGSSIAE---NLEIHSYGNELNAIGSKGLYRGVSELGEL 1357
             +S   S  E+P+++L ++  M S+  +      I +YG  L  +  + + +G S+L + 
Sbjct: 237  SDS-DCSCFEEPESVLDLMMVMNSNSQQQDSGFLIFNYGPILECLKRRVICKGTSQLSDF 295

Query: 1358 SMNSFRAGITLQL--MEAXXXXXXXXXXXXRMEENQAKTKRRKKDISNHRNKLSEIKISL 1531
             +N  +AGI LQL  +               MEE    +  +K +  +   KLS+IK  +
Sbjct: 296  GVNQLQAGIILQLEAIGIGGQQTSNMSFLRNMEERVKASFAKKTNAFDPGKKLSKIKEDM 355

Query: 1532 IYMEWYMKSRKKYGGYYDSYK--------VQSTREEIVS-HRVIYTHKLKLNQFWRKTVT 1684
             Y+EWY K+    GGYY  +K        V  +REEIV  HR+       L ++W++ V 
Sbjct: 356  AYLEWYKKATLNEGGYYICFKERGSGSRDVVKSREEIVKRHRI-------LTKYWKRMVA 408

Query: 1685 ENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYTE-QRPNHYKLLEKWL 1861
            E + +P +E A  R  WL   TNYRR+VEPL+IA++Y KGN +Y    R  HYKLLEKW+
Sbjct: 409  EVDKLPQKEEAAFRTRWLYGGTNYRRMVEPLEIAEYYMKGNRDYVNLGRSEHYKLLEKWM 468

Query: 1862 NEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGILLGLEGFE 2041
            NED+  +     +  KA +LT+D+CFWA+VEEA+I    LR EGSS+  +     L  F 
Sbjct: 469  NEDK--IGGIANDRRKAVSLTEDSCFWAYVEEAIIASKGLR-EGSSEEKENSRRNLVNFG 525

Query: 2042 AYLMREINGYLVSPDIFMDGSSLM-RWWNEYK-TYRGSMYASEFAQYMNSKSYTSY 2203
             Y+M  I  Y VS +IF   S+ M +WW EY+     S+Y+     YM ++ Y SY
Sbjct: 526  EYVMSLIRSYSVSTEIFQPQSTFMKKWWQEYRQDILSSLYSCPLTFYMENEEYRSY 581


>ref|XP_009764370.1| PREDICTED: senescence-associated carboxylesterase 101-like isoform X2
            [Nicotiana sylvestris]
          Length = 582

 Score =  343 bits (880), Expect = e-105
 Identities = 224/597 (37%), Positives = 330/597 (55%), Gaps = 47/597 (7%)
 Frame = +2

Query: 554  ESEMFSSEVELSNLLGSFDILPDAYQAILKTSS-------------AYQLHTDIQSGIQF 694
            +  +FSS  EL+NL+ S D+L D++  I +  S             A++++    +    
Sbjct: 3    QDSLFSSGQELANLVVSSDLLHDSWATIYELLSHAYLNNPTNLAPIAFKVYYPYNTNGAI 62

Query: 695  LAFNISSEYTTRFLKGEFNLVSSRNHNV----VDFISTKVKPIFSINKAAVDLFENISFE 862
            +AF +SS   T     +  +VSS +        DFISTK+ P FS++K A+ LF ++  +
Sbjct: 63   IAF-VSSPTCTTVHHLQKEMVSSEDLKGSQLDFDFISTKLNPHFSVHKGAIALFASLLNQ 121

Query: 863  LSQIEYQL-TYKPLVVTGRCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPL 1039
            LS ++ QL ++ PL++TG  LGG +A LFTLWL   I +K+     NNKRP CITFGSP 
Sbjct: 122  LSALKLQLDSFSPLIITGHSLGGSVASLFTLWLLDNISLKD-----NNKRPTCITFGSPF 176

Query: 1040 LGNEALQHAICERPTWKSSFLNVVSKKDPVASFFSSYT-----------PYKPFGTFLFC 1186
            LG+  LQ AI ERP+W SSFL+V S +DP+  FF S T            YKPFGTFL C
Sbjct: 177  LGDRGLQQAISERPSWNSSFLHVASNQDPIPRFFISPTNGFSGSIPQSCVYKPFGTFLLC 236

Query: 1187 EESGGNSVLEDPDTILAVLDSMGSSIAENLE----IHSYGNELNAIGSKGLYRGVSELGE 1354
             +S   S  E+P+++L ++ +M +SI++  +    + +YG  L  +  + + +G S+L +
Sbjct: 237  SDS-DCSCFEEPESVLELMMAM-NSISQQQDNGFLVFNYGPILECLKHRVICKGTSQLSD 294

Query: 1355 LSMNSFRAGITLQL--MEAXXXXXXXXXXXXRMEENQAKTKRRKKDISNHRNKLSEIKIS 1528
              ++  +AGI LQL  +               MEE    +  +K +  +   KLS+IK  
Sbjct: 295  FGVSQLQAGIILQLEAIGIGGQQTSNMSFLRSMEERVKASFVKKTNAFDPGKKLSKIKED 354

Query: 1529 LIYMEWYMKSRKKYGGYYDSYK--------VQSTREEIVS-HRVIYTHKLKLNQFWRKTV 1681
            + Y+EWY K+    GG Y  +K        V  +REEIV  HR+       L ++W++ V
Sbjct: 355  MAYLEWYKKATLNEGGCYICFKERGSGSRDVVKSREEIVKRHRI-------LTKYWKRMV 407

Query: 1682 TENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYTE-QRPNHYKLLEKW 1858
             E   +P +E A  R  WL   TNYRR+VEPL+IA++Y KGN +Y    R  HYKLLEKW
Sbjct: 408  AEVEKLPQKEEAAFRTRWLYGGTNYRRMVEPLEIAEYYMKGNRDYVNFGRSEHYKLLEKW 467

Query: 1859 LNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGILLGLEGF 2038
            +N+D+  +     +  KA +LT+D+CFWA+VEEA+I    LR EGSS+  +     L  F
Sbjct: 468  MNKDK--IGGIANDRRKAVSLTEDSCFWAYVEEAIITSKGLR-EGSSEEKEKSRRNLVNF 524

Query: 2039 EAYLMREINGYLVSPDIFMDGSSLM-RWWNEYK-TYRGSMYASEFAQYMNSKSYTSY 2203
              Y+M  I  Y VS +IF   S+ M +WW EY+     S+Y+     YM ++ Y SY
Sbjct: 525  GEYVMSLIRSYSVSTEIFQPQSTFMKKWWQEYRQDILSSLYSCPLTFYMENEEYRSY 581


>ref|XP_019223910.1| PREDICTED: senescence-associated carboxylesterase 101-like isoform X1
            [Nicotiana attenuata]
 gb|OIT33729.1| senescence-associated carboxylesterase 101 [Nicotiana attenuata]
          Length = 583

 Score =  342 bits (878), Expect = e-104
 Identities = 225/597 (37%), Positives = 323/597 (54%), Gaps = 47/597 (7%)
 Frame = +2

Query: 554  ESEMFSSEVELSNLLGSFDILPDAYQAILKTSS-------------AYQLHTDIQSGIQF 694
            +  +FSS  EL+NL+   D+L D++  I    S             A++++    +    
Sbjct: 3    QDSLFSSGQELANLVVISDLLHDSWATIYDLISHAYLNNPTNPAPIAFKVYYPYNTNGAI 62

Query: 695  LAFNISSEYTTRFLKGEFNLVSSRNHNV----VDFISTKVKPIFSINKAAVDLFENISFE 862
            +AF +SS   T     +  +VSS +        DFISTK+ P FS++K A+ LF ++  +
Sbjct: 63   VAF-VSSPTCTTLHHLQKEMVSSEDLEGSQLDFDFISTKLNPHFSLHKEAIALFASLLNQ 121

Query: 863  LSQIEYQL-TYKPLVVTGRCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPL 1039
            LS ++ QL ++ PL++TG  LGG +A LFTLWL   I  K+     NNKRP CITFGSP 
Sbjct: 122  LSALKLQLGSFSPLIITGHSLGGSVASLFTLWLLDNISPKD-----NNKRPTCITFGSPF 176

Query: 1040 LGNEALQHAICERPTWKSSFLNVVSKKDPVASF-------FSSYTP----YKPFGTFLFC 1186
            LG+   Q AI ERP+W SSFL+V S +DP+  F       FS   P    YKPFGTFL C
Sbjct: 177  LGDRGFQQAISERPSWNSSFLHVASNQDPIPRFLISPTNGFSGSIPQSCVYKPFGTFLLC 236

Query: 1187 EESGGNSVLEDPDTILAVLDSMGSSIAE---NLEIHSYGNELNAIGSKGLYRGVSELGEL 1357
             +S   S  E+P+++L ++  M S+  +      I +YG  L  +  + + +G S+L + 
Sbjct: 237  SDS-DCSCFEEPESVLDLMMVMNSNSQQQDSGFLIFNYGPILECLKRRVICKGTSQLSDF 295

Query: 1358 SMNSFRAGITLQLME---AXXXXXXXXXXXXRMEENQAKTKRRKKDISNHRNKLSEIKIS 1528
             +N  +AGI LQL                   MEE    +  +K +  +   KLS+IK  
Sbjct: 296  GVNQLQAGIILQLEAIGIGGQQQTSNMSFLRNMEERVKASFAKKTNAFDPGKKLSKIKED 355

Query: 1529 LIYMEWYMKSRKKYGGYYDSYK--------VQSTREEIVS-HRVIYTHKLKLNQFWRKTV 1681
            + Y+EWY K+    GGYY  +K        V  +REEIV  HR+       L ++W++ V
Sbjct: 356  MAYLEWYKKATLNEGGYYICFKERGSGSRDVVKSREEIVKRHRI-------LTKYWKRMV 408

Query: 1682 TENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYTE-QRPNHYKLLEKW 1858
             E + +P +E A  R  WL   TNYRR+VEPL+IA++Y KGN +Y    R  HYKLLEKW
Sbjct: 409  AEVDKLPQKEEAAFRTRWLYGGTNYRRMVEPLEIAEYYMKGNRDYVNLGRSEHYKLLEKW 468

Query: 1859 LNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGILLGLEGF 2038
            +NED+  +     +  KA +LT+D+CFWA+VEEA+I    LR EGSS+  +     L  F
Sbjct: 469  MNEDK--IGGIANDRRKAVSLTEDSCFWAYVEEAIIASKGLR-EGSSEEKENSRRNLVNF 525

Query: 2039 EAYLMREINGYLVSPDIFMDGSSLM-RWWNEYK-TYRGSMYASEFAQYMNSKSYTSY 2203
              Y+M  I  Y VS +IF   S+ M +WW EY+     S+Y+     YM ++ Y SY
Sbjct: 526  GEYVMSLIRSYSVSTEIFQPQSTFMKKWWQEYRQDILSSLYSCPLTFYMENEEYRSY 582


>ref|XP_009764369.1| PREDICTED: senescence-associated carboxylesterase 101-like isoform X1
            [Nicotiana sylvestris]
          Length = 583

 Score =  342 bits (878), Expect = e-104
 Identities = 224/598 (37%), Positives = 329/598 (55%), Gaps = 48/598 (8%)
 Frame = +2

Query: 554  ESEMFSSEVELSNLLGSFDILPDAYQAILKTSS-------------AYQLHTDIQSGIQF 694
            +  +FSS  EL+NL+ S D+L D++  I +  S             A++++    +    
Sbjct: 3    QDSLFSSGQELANLVVSSDLLHDSWATIYELLSHAYLNNPTNLAPIAFKVYYPYNTNGAI 62

Query: 695  LAFNISSEYTTRFLKGEFNLVSSRNHNV----VDFISTKVKPIFSINKAAVDLFENISFE 862
            +AF +SS   T     +  +VSS +        DFISTK+ P FS++K A+ LF ++  +
Sbjct: 63   IAF-VSSPTCTTVHHLQKEMVSSEDLKGSQLDFDFISTKLNPHFSVHKGAIALFASLLNQ 121

Query: 863  LSQIEYQL-TYKPLVVTGRCLGGYLAILFTLWLQQAIDMKELNGYTNNKRPICITFGSPL 1039
            LS ++ QL ++ PL++TG  LGG +A LFTLWL   I +K+     NNKRP CITFGSP 
Sbjct: 122  LSALKLQLDSFSPLIITGHSLGGSVASLFTLWLLDNISLKD-----NNKRPTCITFGSPF 176

Query: 1040 LGNEALQHAICERPTWKSSFLNVVSKKDPVASFFSSYT-----------PYKPFGTFLFC 1186
            LG+  LQ AI ERP+W SSFL+V S +DP+  FF S T            YKPFGTFL C
Sbjct: 177  LGDRGLQQAISERPSWNSSFLHVASNQDPIPRFFISPTNGFSGSIPQSCVYKPFGTFLLC 236

Query: 1187 EESGGNSVLEDPDTILAVLDSMGSSIAENLE----IHSYGNELNAIGSKGLYRGVSELGE 1354
             +S   S  E+P+++L ++ +M +SI++  +    + +YG  L  +  + + +G S+L +
Sbjct: 237  SDS-DCSCFEEPESVLELMMAM-NSISQQQDNGFLVFNYGPILECLKHRVICKGTSQLSD 294

Query: 1355 LSMNSFRAGITLQLME---AXXXXXXXXXXXXRMEENQAKTKRRKKDISNHRNKLSEIKI 1525
              ++  +AGI LQL                   MEE    +  +K +  +   KLS+IK 
Sbjct: 295  FGVSQLQAGIILQLEAIGIGGQQQTSNMSFLRSMEERVKASFVKKTNAFDPGKKLSKIKE 354

Query: 1526 SLIYMEWYMKSRKKYGGYYDSYK--------VQSTREEIVS-HRVIYTHKLKLNQFWRKT 1678
             + Y+EWY K+    GG Y  +K        V  +REEIV  HR+       L ++W++ 
Sbjct: 355  DMAYLEWYKKATLNEGGCYICFKERGSGSRDVVKSREEIVKRHRI-------LTKYWKRM 407

Query: 1679 VTENNLMPPQEAAKVRKGWLSSATNYRRIVEPLDIADHYKKGNSNYTE-QRPNHYKLLEK 1855
            V E   +P +E A  R  WL   TNYRR+VEPL+IA++Y KGN +Y    R  HYKLLEK
Sbjct: 408  VAEVEKLPQKEEAAFRTRWLYGGTNYRRMVEPLEIAEYYMKGNRDYVNFGRSEHYKLLEK 467

Query: 1856 WLNEDQQDLNPSEGEYIKAATLTKDTCFWAHVEEALILLNDLRNEGSSDNIDGILLGLEG 2035
            W+N+D+  +     +  KA +LT+D+CFWA+VEEA+I    LR EGSS+  +     L  
Sbjct: 468  WMNKDK--IGGIANDRRKAVSLTEDSCFWAYVEEAIITSKGLR-EGSSEEKEKSRRNLVN 524

Query: 2036 FEAYLMREINGYLVSPDIFMDGSSLM-RWWNEYK-TYRGSMYASEFAQYMNSKSYTSY 2203
            F  Y+M  I  Y VS +IF   S+ M +WW EY+     S+Y+     YM ++ Y SY
Sbjct: 525  FGEYVMSLIRSYSVSTEIFQPQSTFMKKWWQEYRQDILSSLYSCPLTFYMENEEYRSY 582