BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00005874
         (1504 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KVI12435.1| Zinc finger, RING/FYVE/PHD-type [Cynara carduncul...   381   e-122
ref|XP_021968981.1| putative E3 ubiquitin-protein ligase RF298 [...   357   e-113
gb|OTG21756.1| putative zinc finger, RING/FYVE/PHD-type [Heliant...   357   e-113
gb|PLY61778.1| hypothetical protein LSAT_8X55580 [Lactuca sativa]     311   2e-95
ref|XP_023749584.1| putative E3 ubiquitin-protein ligase RF298 [...   311   3e-95
ref|XP_015168014.1| PREDICTED: putative E3 ubiquitin-protein lig...   246   3e-69
ref|XP_010321841.1| PREDICTED: putative E3 ubiquitin-protein lig...   246   3e-69
ref|XP_006355787.1| PREDICTED: putative E3 ubiquitin-protein lig...   246   4e-69
ref|XP_015078209.1| PREDICTED: putative E3 ubiquitin-protein lig...   245   9e-69
gb|OMP06663.1| putative E3 ubiquitin-protein ligase [Corchorus c...   244   3e-68
ref|XP_015078210.1| PREDICTED: putative E3 ubiquitin-protein lig...   240   6e-67
ref|XP_023889471.1| putative E3 ubiquitin-protein ligase RF298 i...   238   3e-66
gb|POE64780.1| putative e3 ubiquitin-protein ligase [Quercus suber]   238   5e-66
ref|XP_023889470.1| putative E3 ubiquitin-protein ligase RF298 i...   238   6e-66
ref|XP_004290694.1| PREDICTED: putative E3 ubiquitin-protein lig...   236   3e-65
ref|XP_008360889.2| PREDICTED: putative E3 ubiquitin-protein lig...   234   3e-65
ref|XP_008360888.2| PREDICTED: putative E3 ubiquitin-protein lig...   234   3e-65
ref|XP_021803473.1| putative E3 ubiquitin-protein ligase RF298 [...   229   5e-65
ref|XP_021281192.1| putative E3 ubiquitin-protein ligase RF298 [...   234   1e-64
ref|XP_017184827.1| PREDICTED: putative E3 ubiquitin-protein lig...   234   2e-64

>gb|KVI12435.1| Zinc finger, RING/FYVE/PHD-type [Cynara cardunculus var. scolymus]
          Length = 669

 Score =  381 bits (978), Expect = e-122
 Identities = 220/442 (49%), Positives = 273/442 (61%), Gaps = 28/442 (6%)
 Frame = +1

Query: 7    KKSFRFERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVNNKNPLPLKETNS- 183
            +K FRFE+ Y GRMSKKALK  L +W   +L  +K   +CESSS       L LKET   
Sbjct: 231  EKMFRFEKPYKGRMSKKALKEKLAAWG--DLVSDKKHPSCESSSGTKKTPTLALKETEET 288

Query: 184  -------EPVSQ----EPLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQK 330
                   EPV+     EP KT ++YL+ IP+DE+KGEHV +D KD+++L A   + +L K
Sbjct: 289  AKATPKPEPVAPVAAPEPTKTVQHYLASIPYDESKGEHVPRDVKDKILLAAVDQVLALHK 348

Query: 331  ELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXX-----------SETTA 477
            EL+DWDDWAN+KVMQVAKRLS DR                               SE T 
Sbjct: 349  ELRDWDDWANLKVMQVAKRLSQDRAELNKLRAEKEEAEKMMKDKQVAEENNMRKLSEMTT 408

Query: 478  ALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXX 657
            ALN              RL+ E  +LK++ E A  RS  EA+ L+ ALQKEQEA +K   
Sbjct: 409  ALNNANYQIEMASSAVVRLKSEKIILKKEMEAARIRSSIEAKRLEAALQKEQEAYKKSQS 468

Query: 658  XXXXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSE 837
                              VVP QR  EKAK LL++T+IRLA E+KETAK   + ES++ E
Sbjct: 469  FGTEKSLLEEELKALKHEVVPIQRSAEKAKDLLSQTQIRLANEEKETAKVVLKYESLKRE 528

Query: 838  REHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSWQP 1017
            RE +E +AK+EE++  EKAE +L+++E+E+KR+Q EI A+K E ETKKIAAMR  M+W P
Sbjct: 529  RELIEEMAKSEEEMVVEKAEKKLQKFENEMKRIQYEISAMKLEQETKKIAAMRTGMNW-P 587

Query: 1018 AELTSSQSEV-----KHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNG 1182
                S  SE+     K+G    KKDRECVMCLTEEMTVV VPCGHQVLCA+CNV+HE+NG
Sbjct: 588  VFEGSKSSEILRNNNKNGNLRQKKDRECVMCLTEEMTVVLVPCGHQVLCADCNVIHEQNG 647

Query: 1183 MKDCPSCRTQIQKRITARFAKS 1248
            MKDCPSCRT IQKRI ARFAKS
Sbjct: 648  MKDCPSCRTPIQKRINARFAKS 669


>ref|XP_021968981.1| putative E3 ubiquitin-protein ligase RF298 [Helianthus annuus]
 ref|XP_021968982.1| putative E3 ubiquitin-protein ligase RF298 [Helianthus annuus]
          Length = 600

 Score =  357 bits (916), Expect = e-113
 Identities = 216/429 (50%), Positives = 265/429 (61%), Gaps = 13/429 (3%)
 Frame = +1

Query: 1    GHKKSFR-FERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVNNKNPLP-LKE 174
            G+K++   FE+ + G+MSKKALKA +     +NL   K       S SV+ +NPL  ++E
Sbjct: 195  GNKRNLTSFEKAFKGQMSKKALKAKI-----VNLVDNK------QSPSVSKENPLKEIEE 243

Query: 175  TNSEPVSQEPLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDW 354
               +  + +P KTA+YYL+GIPFDE+KGEHV QDD+D+MILTA + LQSLQK++K WDDW
Sbjct: 244  EKPKVTTPKPFKTADYYLAGIPFDESKGEHVPQDDQDKMILTAVAQLQSLQKQVKSWDDW 303

Query: 355  ANVKVMQVAKRLSYDRP-----------XXXXXXXXXXXXXXXXXXXSETTAALNTXXXX 501
            A+VKVMQVA RLS D P                              SE TAAL T    
Sbjct: 304  ASVKVMQVANRLSQDGPEIKKLKAEKVEAENLKKDKEATEESTKKKLSEMTAALTTTDCQ 363

Query: 502  XXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXX 681
                     RLE ENSVLK+D+E+AE RSL EA SL EAL KE E ++K           
Sbjct: 364  FKMSTSTIARLESENSVLKRDKERAEKRSLIEANSLAEALCKELEVLKKSELCGSEKKLL 423

Query: 682  XXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALA 861
                      V P QRDL+KAK LL ETE RLA+E++E AKF + A+SIR+ERE LEALA
Sbjct: 424  EEELKSLKQEVGPNQRDLDKAKLLLKETETRLAQEEREAAKFYEEAKSIRNERERLEALA 483

Query: 862  KAEEQITAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSWQPAELTSSQS 1041
            +A  +   ++A  ELK+ E+EIKRL+ EI A+  EAETKKIA     MSWQ         
Sbjct: 484  EAGGERAMKRAAKELKKVETEIKRLEYEIAALNLEAETKKIAG---GMSWQ--------- 531

Query: 1042 EVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQK 1221
                G +    D ECVMCL+EEMTV+FVPCGHQVLC ECNV+HE NGMK+CPSCRT IQK
Sbjct: 532  ---LGCEGLSHD-ECVMCLSEEMTVLFVPCGHQVLCDECNVIHEDNGMKECPSCRTPIQK 587

Query: 1222 RITARFAKS 1248
            RI   FAKS
Sbjct: 588  RINCLFAKS 596


>gb|OTG21756.1| putative zinc finger, RING/FYVE/PHD-type [Helianthus annuus]
          Length = 620

 Score =  357 bits (916), Expect = e-113
 Identities = 216/429 (50%), Positives = 265/429 (61%), Gaps = 13/429 (3%)
 Frame = +1

Query: 1    GHKKSFR-FERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVNNKNPLP-LKE 174
            G+K++   FE+ + G+MSKKALKA +     +NL   K       S SV+ +NPL  ++E
Sbjct: 215  GNKRNLTSFEKAFKGQMSKKALKAKI-----VNLVDNK------QSPSVSKENPLKEIEE 263

Query: 175  TNSEPVSQEPLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDW 354
               +  + +P KTA+YYL+GIPFDE+KGEHV QDD+D+MILTA + LQSLQK++K WDDW
Sbjct: 264  EKPKVTTPKPFKTADYYLAGIPFDESKGEHVPQDDQDKMILTAVAQLQSLQKQVKSWDDW 323

Query: 355  ANVKVMQVAKRLSYDRP-----------XXXXXXXXXXXXXXXXXXXSETTAALNTXXXX 501
            A+VKVMQVA RLS D P                              SE TAAL T    
Sbjct: 324  ASVKVMQVANRLSQDGPEIKKLKAEKVEAENLKKDKEATEESTKKKLSEMTAALTTTDCQ 383

Query: 502  XXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXX 681
                     RLE ENSVLK+D+E+AE RSL EA SL EAL KE E ++K           
Sbjct: 384  FKMSTSTIARLESENSVLKRDKERAEKRSLIEANSLAEALCKELEVLKKSELCGSEKKLL 443

Query: 682  XXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALA 861
                      V P QRDL+KAK LL ETE RLA+E++E AKF + A+SIR+ERE LEALA
Sbjct: 444  EEELKSLKQEVGPNQRDLDKAKLLLKETETRLAQEEREAAKFYEEAKSIRNERERLEALA 503

Query: 862  KAEEQITAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSWQPAELTSSQS 1041
            +A  +   ++A  ELK+ E+EIKRL+ EI A+  EAETKKIA     MSWQ         
Sbjct: 504  EAGGERAMKRAAKELKKVETEIKRLEYEIAALNLEAETKKIAG---GMSWQ--------- 551

Query: 1042 EVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQK 1221
                G +    D ECVMCL+EEMTV+FVPCGHQVLC ECNV+HE NGMK+CPSCRT IQK
Sbjct: 552  ---LGCEGLSHD-ECVMCLSEEMTVLFVPCGHQVLCDECNVIHEDNGMKECPSCRTPIQK 607

Query: 1222 RITARFAKS 1248
            RI   FAKS
Sbjct: 608  RINCLFAKS 616


>gb|PLY61778.1| hypothetical protein LSAT_8X55580 [Lactuca sativa]
          Length = 656

 Score =  311 bits (798), Expect = 2e-95
 Identities = 196/435 (45%), Positives = 260/435 (59%), Gaps = 21/435 (4%)
 Frame = +1

Query: 7    KKSFRFERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVN---NKNP-LPLKE 174
            K +   E+ Y GR  +K LK  + S K+L+L  +K  S+  SSSS +   NKN  L LKE
Sbjct: 225  KAALLVEKPYKGRRIRKGLKKQVFSLKELSLVNKKSPSSSSSSSSSSSEQNKNTSLALKE 284

Query: 175  TNSEPV----SQEPLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKD 342
            T  +P     S++P KT +YYL  IP DE KGE+V +DDKD + L A + +++LQKEL+ 
Sbjct: 285  TEEKPKGTPKSEQP-KTLDYYLKNIPCDE-KGEYVPRDDKDILRLEAVAQIEALQKELQG 342

Query: 343  WDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXXS----ETTAALNTXXXXXXX 510
            WDDWA  KVM+V KRL+ +R                    +    ETT  LNT       
Sbjct: 343  WDDWATSKVMEVTKRLNQNRSEVNMLKAEMKDNEAMDENTAKRLLETTLTLNTTNSELNV 402

Query: 511  XXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXX 690
                  +L+ E SV+K++ E+  NRS+ +A+ L++AL KE+EA +K              
Sbjct: 403  ANSTIVKLKTEQSVIKEEMERERNRSIFQAKRLEQALIKEEEARKKSQSAFGSEKTSLEE 462

Query: 691  XXXXXXXVV-PKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKA 867
                    V  KQ++LEKAK LL+ET IR+AKE++ETAK  ++AE I++ERE +EALA A
Sbjct: 463  ELKVLKRQVGQKQQELEKAKCLLSETLIRVAKEERETAKVVNKAECIKNERERIEALAMA 522

Query: 868  EEQITAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSW--------QPAE 1023
            E +I   KAE E K+ E+E KR++ EI A+K EAE+K+IA M   M+         +   
Sbjct: 523  EYEIMKGKAEEEFKKNETEKKRIEYEISALKLEAESKRIAEMYMSMNMNMDSSKGLEGFR 582

Query: 1024 LTSSQSEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSC 1203
              S + + K   K  KKDRECVMCL EEM+VVFVPCGHQVLC  CNV HE+  M +CPSC
Sbjct: 583  NMSKKKKKKKKMKKKKKDRECVMCLNEEMSVVFVPCGHQVLCKACNVKHEER-MNECPSC 641

Query: 1204 RTQIQKRITARFAKS 1248
            RT IQKRI ARFAKS
Sbjct: 642  RTPIQKRINARFAKS 656


>ref|XP_023749584.1| putative E3 ubiquitin-protein ligase RF298 [Lactuca sativa]
          Length = 663

 Score =  311 bits (798), Expect = 3e-95
 Identities = 196/435 (45%), Positives = 260/435 (59%), Gaps = 21/435 (4%)
 Frame = +1

Query: 7    KKSFRFERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVN---NKNP-LPLKE 174
            K +   E+ Y GR  +K LK  + S K+L+L  +K  S+  SSSS +   NKN  L LKE
Sbjct: 232  KAALLVEKPYKGRRIRKGLKKQVFSLKELSLVNKKSPSSSSSSSSSSSEQNKNTSLALKE 291

Query: 175  TNSEPV----SQEPLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKD 342
            T  +P     S++P KT +YYL  IP DE KGE+V +DDKD + L A + +++LQKEL+ 
Sbjct: 292  TEEKPKGTPKSEQP-KTLDYYLKNIPCDE-KGEYVPRDDKDILRLEAVAQIEALQKELQG 349

Query: 343  WDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXXS----ETTAALNTXXXXXXX 510
            WDDWA  KVM+V KRL+ +R                    +    ETT  LNT       
Sbjct: 350  WDDWATSKVMEVTKRLNQNRSEVNMLKAEMKDNEAMDENTAKRLLETTLTLNTTNSELNV 409

Query: 511  XXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXX 690
                  +L+ E SV+K++ E+  NRS+ +A+ L++AL KE+EA +K              
Sbjct: 410  ANSTIVKLKTEQSVIKEEMERERNRSIFQAKRLEQALIKEEEARKKSQSAFGSEKTSLEE 469

Query: 691  XXXXXXXVV-PKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKA 867
                    V  KQ++LEKAK LL+ET IR+AKE++ETAK  ++AE I++ERE +EALA A
Sbjct: 470  ELKVLKRQVGQKQQELEKAKCLLSETLIRVAKEERETAKVVNKAECIKNERERIEALAMA 529

Query: 868  EEQITAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSW--------QPAE 1023
            E +I   KAE E K+ E+E KR++ EI A+K EAE+K+IA M   M+         +   
Sbjct: 530  EYEIMKGKAEEEFKKNETEKKRIEYEISALKLEAESKRIAEMYMSMNMNMDSSKGLEGFR 589

Query: 1024 LTSSQSEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSC 1203
              S + + K   K  KKDRECVMCL EEM+VVFVPCGHQVLC  CNV HE+  M +CPSC
Sbjct: 590  NMSKKKKKKKKMKKKKKDRECVMCLNEEMSVVFVPCGHQVLCKACNVKHEER-MNECPSC 648

Query: 1204 RTQIQKRITARFAKS 1248
            RT IQKRI ARFAKS
Sbjct: 649  RTPIQKRINARFAKS 663


>ref|XP_015168014.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Solanum tuberosum]
          Length = 819

 Score =  246 bits (629), Expect = 3e-69
 Identities = 161/490 (32%), Positives = 245/490 (50%), Gaps = 74/490 (15%)
 Frame = +1

Query: 7    KKSFRFERTYSGRMSKKALKANLTSWKKLNLS---------------------IEKVQSA 123
            +K+F FE++Y GRM K + KA LT+W  + L                         V+S 
Sbjct: 330  QKTFHFEKSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESRSSGLGMKSTNSKVTTTVKSN 389

Query: 124  C-----ESSSSVNNKNPLPLKETNSEPVSQEPL--------------------------- 207
            C      S SS  + +  P  ET + P +Q+ +                           
Sbjct: 390  CPLAVGSSHSSSTSPSIAPSSETATVPATQDTVCALPAVNTNIAASLTPDPKSSSNTPGS 449

Query: 208  -----KTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVM 372
                 K  +YY +GIP+DE+  +HV Q++KD+++L   + +Q+LQKEL+ W DWAN KVM
Sbjct: 450  TPALPKVLDYY-AGIPYDESLEKHVPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVM 508

Query: 373  QVAKRLSYDR-----------PXXXXXXXXXXXXXXXXXXXSETTAALNTXXXXXXXXXX 519
            Q A+RL  D+                               SE   AL+           
Sbjct: 509  QAARRLGKDQGELKMLKQEKEEAEKLQKEKQIVEENNMKRLSEMEYALSNASGQSKMADS 568

Query: 520  XXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXX 699
               RL+ EN  LK++ + A   +L  A +  +A+ +EQE ++KC                
Sbjct: 569  TLHRLKEENVSLKKEMDAATLAALESATNFHQAVAREQEILKKCQAWEMKKGSLLDNFST 628

Query: 700  XXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQI 879
                 V  +++LE+AK    + ++   +E++E  K   +A+S+++ERE     +K EE  
Sbjct: 629  LKREAVHFEQELERAKTRQNQLKVLSEQEEREKRKVLQQADSLKAEREKRGVQSKMEEDN 688

Query: 880  TAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSW---QPAELTSSQS--E 1044
              EKAE  +++ + +I++L+ EI  ++ ++E  KI A+R+ ++    Q  +LT S +  E
Sbjct: 689  IREKAERNMQKCKEDIRKLESEISLLRFQSEGSKIEALRRGINNTRPQSPKLTKSLAVFE 748

Query: 1045 VKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKR 1224
               G+ S K +RECVMCL+EEMTVVF+PC HQVLCA+CNVLHEK GM DCPSCRT I+KR
Sbjct: 749  ENLGSGSVKIERECVMCLSEEMTVVFLPCAHQVLCAQCNVLHEKQGMNDCPSCRTPIKKR 808

Query: 1225 ITARFAKS*R 1254
            I  +FA+S R
Sbjct: 809  INVQFARSSR 818


>ref|XP_010321841.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Solanum
            lycopersicum]
          Length = 842

 Score =  246 bits (629), Expect = 3e-69
 Identities = 158/490 (32%), Positives = 245/490 (50%), Gaps = 74/490 (15%)
 Frame = +1

Query: 7    KKSFRFERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVNNKN---------- 156
            +K+F FE++Y GRM K + KA LT+W  + L       +C S  ++ + N          
Sbjct: 353  QKTFHFEKSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESCSSGLAMKSTNSKVTTTVKCN 412

Query: 157  -PL---------------PLKETNSEPVSQE----------------------------- 201
             PL               P  ET S P +Q+                             
Sbjct: 413  GPLEVGSSHSSSTYPSIAPSSETASVPATQDTVCALPAVNTNISVPLTPDPKSSSNTPGS 472

Query: 202  ---PLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVM 372
               P K  +YY +GIP+DE+ G++V Q++KD+++L   + +Q+LQKEL+ W DWAN KVM
Sbjct: 473  TPAPPKVLDYY-AGIPYDESLGKYVPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVM 531

Query: 373  QVAKRLSYDR-----------PXXXXXXXXXXXXXXXXXXXSETTAALNTXXXXXXXXXX 519
            Q A+RL  D+                               SE   AL+           
Sbjct: 532  QAARRLGKDQGELKMLKQEKEEAEKLQKEKQMVEENTMKRLSEMECALSNASGQSKMADS 591

Query: 520  XXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXX 699
               RL+ EN  LK++ + A   +L  A ++ +A+ +EQE ++ C                
Sbjct: 592  TLHRLKEENVSLKKEMDAATLAALESATNVHQAVAREQEMLKNCQALEMENGSLQHNFST 651

Query: 700  XXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQI 879
                 V  +++LE+AK    + ++ L +E++E  +   +A+S+++ERE     +K EE  
Sbjct: 652  LKREAVHFEQELERAKTRQNQLKVLLEQEEREKRRVLQQADSLKAEREKRGVQSKMEEDN 711

Query: 880  TAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSW---QPAELTSSQS--E 1044
              E  E  +++ + +IK+L+ EI  ++ ++E  KI A+R+ ++    Q  +LT S +  E
Sbjct: 712  IRETTERNMQKCKEDIKKLESEISLLRYQSEGSKIEALRRGINHTRPQSPKLTKSLAVFE 771

Query: 1045 VKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKR 1224
               G+ S + +RECVMCL+EEMTVVF+PC HQVLCA+C+VLHEK GM DCPSCRT I+KR
Sbjct: 772  ENLGSASVEIERECVMCLSEEMTVVFLPCAHQVLCAQCSVLHEKQGMNDCPSCRTPIKKR 831

Query: 1225 ITARFAKS*R 1254
            I  +FA+S R
Sbjct: 832  INVQFARSSR 841


>ref|XP_006355787.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Solanum tuberosum]
 ref|XP_006355788.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Solanum tuberosum]
          Length = 845

 Score =  246 bits (629), Expect = 4e-69
 Identities = 161/490 (32%), Positives = 245/490 (50%), Gaps = 74/490 (15%)
 Frame = +1

Query: 7    KKSFRFERTYSGRMSKKALKANLTSWKKLNLS---------------------IEKVQSA 123
            +K+F FE++Y GRM K + KA LT+W  + L                         V+S 
Sbjct: 356  QKTFHFEKSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESRSSGLGMKSTNSKVTTTVKSN 415

Query: 124  C-----ESSSSVNNKNPLPLKETNSEPVSQEPL--------------------------- 207
            C      S SS  + +  P  ET + P +Q+ +                           
Sbjct: 416  CPLAVGSSHSSSTSPSIAPSSETATVPATQDTVCALPAVNTNIAASLTPDPKSSSNTPGS 475

Query: 208  -----KTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVM 372
                 K  +YY +GIP+DE+  +HV Q++KD+++L   + +Q+LQKEL+ W DWAN KVM
Sbjct: 476  TPALPKVLDYY-AGIPYDESLEKHVPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVM 534

Query: 373  QVAKRLSYDR-----------PXXXXXXXXXXXXXXXXXXXSETTAALNTXXXXXXXXXX 519
            Q A+RL  D+                               SE   AL+           
Sbjct: 535  QAARRLGKDQGELKMLKQEKEEAEKLQKEKQIVEENNMKRLSEMEYALSNASGQSKMADS 594

Query: 520  XXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXX 699
               RL+ EN  LK++ + A   +L  A +  +A+ +EQE ++KC                
Sbjct: 595  TLHRLKEENVSLKKEMDAATLAALESATNFHQAVAREQEILKKCQAWEMKKGSLLDNFST 654

Query: 700  XXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQI 879
                 V  +++LE+AK    + ++   +E++E  K   +A+S+++ERE     +K EE  
Sbjct: 655  LKREAVHFEQELERAKTRQNQLKVLSEQEEREKRKVLQQADSLKAEREKRGVQSKMEEDN 714

Query: 880  TAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSW---QPAELTSSQS--E 1044
              EKAE  +++ + +I++L+ EI  ++ ++E  KI A+R+ ++    Q  +LT S +  E
Sbjct: 715  IREKAERNMQKCKEDIRKLESEISLLRFQSEGSKIEALRRGINNTRPQSPKLTKSLAVFE 774

Query: 1045 VKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKR 1224
               G+ S K +RECVMCL+EEMTVVF+PC HQVLCA+CNVLHEK GM DCPSCRT I+KR
Sbjct: 775  ENLGSGSVKIERECVMCLSEEMTVVFLPCAHQVLCAQCNVLHEKQGMNDCPSCRTPIKKR 834

Query: 1225 ITARFAKS*R 1254
            I  +FA+S R
Sbjct: 835  INVQFARSSR 844


>ref|XP_015078209.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Solanum pennellii]
          Length = 842

 Score =  245 bits (626), Expect = 9e-69
 Identities = 158/490 (32%), Positives = 244/490 (49%), Gaps = 74/490 (15%)
 Frame = +1

Query: 7    KKSFRFERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVNNKN---------- 156
            +K+F FE++Y GRM K + KA LT+W  + L       +C S  ++ + N          
Sbjct: 353  QKTFHFEKSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESCSSGLAMKSTNSKVTTTVKCN 412

Query: 157  -PL---------------PLKETNSEPVSQE----------------------------- 201
             PL               P  ET S P +Q+                             
Sbjct: 413  VPLEVASSHSLSTSPSIAPSSETASVPATQDTVCALPAVNTNISVSLTPDPKSSSNTPGS 472

Query: 202  ---PLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVM 372
               P K  +YY +GIP+DE+ G++V Q++KD+++L   + +Q+LQKEL+ W DWAN KVM
Sbjct: 473  TPAPPKVLDYY-AGIPYDESLGKYVPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVM 531

Query: 373  QVAKRLSYDR-----------PXXXXXXXXXXXXXXXXXXXSETTAALNTXXXXXXXXXX 519
            Q A+RL  D+                               SE   AL+           
Sbjct: 532  QAARRLGKDQVELKMLKQEKEEAEKLQKEKQMVEENNMKRLSEMEYALSNASGQSKMADS 591

Query: 520  XXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXX 699
               RL+ EN  LK++ + A   +L  A +  +A+ +EQE ++ C                
Sbjct: 592  TLHRLKEENVSLKKEMDAATLAALESATNFHQAVAREQEMLKNCQAWEMEKGSLQHNFST 651

Query: 700  XXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQI 879
                 V  +++LE+AK    + ++ L +E++E  +   +A+S+++ERE     +K EE  
Sbjct: 652  LKREAVHFEQELERAKTRQNQLKVLLEQEEREKRRVLQQADSLKAEREKRGVQSKMEEDN 711

Query: 880  TAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSW---QPAELTSSQS--E 1044
              E  E  +++ + +IK+L+ EI  ++ ++E  KI A+R+ ++    Q  +LT S +  E
Sbjct: 712  IRETTERNMQKCKEDIKKLESEISLLRFQSEGSKIEALRRGINHTRPQSPKLTKSLAVFE 771

Query: 1045 VKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKR 1224
               G+ S + +RECVMCL+EEMTVVF+PC HQVLCA+C+VLHEK GM DCPSCRT I+KR
Sbjct: 772  ENLGSASVEIERECVMCLSEEMTVVFLPCAHQVLCAQCSVLHEKQGMNDCPSCRTPIKKR 831

Query: 1225 ITARFAKS*R 1254
            I  +FA+S R
Sbjct: 832  INVQFARSSR 841


>gb|OMP06663.1| putative E3 ubiquitin-protein ligase [Corchorus capsularis]
          Length = 866

 Score =  244 bits (623), Expect = 3e-68
 Identities = 156/425 (36%), Positives = 220/425 (51%), Gaps = 25/425 (5%)
 Frame = +1

Query: 46   MSKKALKANLTSWKKLNLSIEKVQSACESSSSVNNKNPLPLKETNSEPVSQEPLKTAEYY 225
            +SK   + +++S    N   +K  S  ESSSSV++K P                   +YY
Sbjct: 461  VSKSEGRTSVSSKTPDNDGEKKPTSKAESSSSVSSKMP-------------------DYY 501

Query: 226  LSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDRP 405
             +GIP+DE+ G+++ QD+KDE+IL     LQ LQ EL  W  WAN K+MQ A+RLS D+P
Sbjct: 502  -AGIPYDESLGKYMPQDEKDELILKLVPRLQELQNELHSWTQWANQKIMQAARRLSKDQP 560

Query: 406  XXXXXXXXXXXXXXXXXXX-----------SETTAALNTXXXXXXXXXXXXFRLEYENSV 552
                                          SE   ALN              +LE E+S+
Sbjct: 561  ELKSLRQEKEEVEQLKKEKLAMEENTMKRLSEMEFALNNATSQVEDAHFTVQKLEREHSL 620

Query: 553  LKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXXXXXXVVPKQRD 732
            LKQ+ E A  ++   A S + +L +EQ AM+                      +V  QR 
Sbjct: 621  LKQELEVATLQAAHAAASCQVSLLREQTAMKDAQSWDGQRSVLQEELTSEKQNLVELQRK 680

Query: 733  LEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKE 912
            + KAK +  +TE+RL +E     KF  +A SIR E+E LEA AKAEE    +KAE ++++
Sbjct: 681  VGKAKNIYNQTEMRLKQESTAKEKFLAQAASIRKEQERLEAAAKAEEDKIKQKAEKDMQK 740

Query: 913  YESEIKRLQREIDAIKSEAETKKIAAMRKEMSW---------QPAELTSSQSEVKH---- 1053
            Y  EIK L+ ++  +K + ++ KIAA+R+             Q  E+TS  + V      
Sbjct: 741  YVEEIKELENKLSELKLKLDSSKIAALRRGTGGVKGQCSSVNQGNEITSFSTRVVDIKDY 800

Query: 1054 -GTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKRIT 1230
             G +  K++ ECVMCL+EE   VF+PC HQVLC +CN LHEK GMKDCP+CRT I++R+ 
Sbjct: 801  SGNRGLKQEHECVMCLSEEKITVFLPCAHQVLCVKCNELHEKQGMKDCPACRTPIKRRLC 860

Query: 1231 ARFAK 1245
            ARFAK
Sbjct: 861  ARFAK 865


>ref|XP_015078210.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Solanum pennellii]
          Length = 837

 Score =  240 bits (613), Expect = 6e-67
 Identities = 159/490 (32%), Positives = 241/490 (49%), Gaps = 74/490 (15%)
 Frame = +1

Query: 7    KKSFRFERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVNNKN---------- 156
            +K+F FE++Y GRM K + KA LT+W  + L       +C S  ++ + N          
Sbjct: 353  QKTFHFEKSYKGRMGKGSFKAKLTTWSSMVLDKTLNSESCSSGLAMKSTNSKVTTTVKCN 412

Query: 157  -PL---------------PLKETNSEPVSQE----------------------------- 201
             PL               P  ET S P +Q+                             
Sbjct: 413  VPLEVASSHSLSTSPSIAPSSETASVPATQDTVCALPAVNTNISVSLTPDPKSSSNTPGS 472

Query: 202  ---PLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVM 372
               P K  +YY +GIP+DE+ G++V Q++KD+++L   + +Q+LQKEL+ W DWAN KVM
Sbjct: 473  TPAPPKVLDYY-AGIPYDESLGKYVPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVM 531

Query: 373  QVAKRLSYDR-----------PXXXXXXXXXXXXXXXXXXXSETTAALNTXXXXXXXXXX 519
            Q A+RL  D+                               SE   AL+           
Sbjct: 532  QAARRLGKDQVELKMLKQEKEEAEKLQKEKQMVEENNMKRLSEMEYALSNASGQSKMADS 591

Query: 520  XXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXX 699
               RL+ EN  LK++ + A   +L  A +  +A+ +EQE ++ C                
Sbjct: 592  TLHRLKEENVSLKKEMDAATLAALESATNFHQAVAREQEMLKNCQAWEMEKGSLQHNFST 651

Query: 700  XXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQI 879
                 V  +++LE+AK     T     KE++E  +   +A+S+++ERE     +K EE  
Sbjct: 652  LKREAVHFEQELERAK-----TRQNQLKEEREKRRVLQQADSLKAEREKRGVQSKMEEDN 706

Query: 880  TAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSW---QPAELTSSQS--E 1044
              E  E  +++ + +IK+L+ EI  ++ ++E  KI A+R+ ++    Q  +LT S +  E
Sbjct: 707  IRETTERNMQKCKEDIKKLESEISLLRFQSEGSKIEALRRGINHTRPQSPKLTKSLAVFE 766

Query: 1045 VKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKR 1224
               G+ S + +RECVMCL+EEMTVVF+PC HQVLCA+C+VLHEK GM DCPSCRT I+KR
Sbjct: 767  ENLGSASVEIERECVMCLSEEMTVVFLPCAHQVLCAQCSVLHEKQGMNDCPSCRTPIKKR 826

Query: 1225 ITARFAKS*R 1254
            I  +FA+S R
Sbjct: 827  INVQFARSSR 836


>ref|XP_023889471.1| putative E3 ubiquitin-protein ligase RF298 isoform X2 [Quercus suber]
 ref|XP_023889472.1| putative E3 ubiquitin-protein ligase RF298 isoform X2 [Quercus suber]
          Length = 834

 Score =  238 bits (608), Expect = 3e-66
 Identities = 144/399 (36%), Positives = 210/399 (52%), Gaps = 14/399 (3%)
 Frame = +1

Query: 88   KLNLSIEKVQSACESSSSVNNKNPLPLKETNSEPVSQEPLKTAEYYLSGIPFDEAKGEHV 267
            K+  ++   ++A  S+SS       P  + N+    Q P    +YY +GIP+D++ G++V
Sbjct: 439  KIPSTLPATENAPTSTSSTAEMKSNPKAQVNTPKAPQIP----DYY-AGIPYDKSLGKYV 493

Query: 268  AQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXX 447
             QD+KDE++L     LQ LQ EL+ W +WAN KVMQ A RL  D+               
Sbjct: 494  PQDEKDELVLKLVPRLQELQDELQCWTEWANQKVMQAAHRLGKDQAELKALRLEKEEAEE 553

Query: 448  XXXXX-----------SETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLT 594
                            SE   AL               RLE +NSVLK++ E A+ R+  
Sbjct: 554  KKKEKKILEENTTKRLSEMENALGNATAEVEIADSTLRRLEVQNSVLKEELEAAKLRASE 613

Query: 595  EAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIR 774
             A S +E +++EQ  ++K                     V   Q++  KAK L  +TE R
Sbjct: 614  SAASYQETVEREQATLKKVQSLEGQKAALQEELGTKKEMVAELQKESGKAKNLYNKTEAR 673

Query: 775  LAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDA 954
              +E+ E      +A SIR ERE LEA AKAEE++  +KAE+E+++Y+ EIK+L++++  
Sbjct: 674  WKQERMEKENLLAQAASIRQERERLEAQAKAEEEMIKQKAESEVQKYKEEIKKLEQKLSQ 733

Query: 955  IKSEAETKKIAAMRKEMSWQPAELTSSQSEVKHGTKSN---KKDRECVMCLTEEMTVVFV 1125
            +K EA++  IAA+R       +   SS  +     ++    K +REC MCL+EE TV+F+
Sbjct: 734  MKMEADSSAIAALRSGTEASSSSTGSSGVQAMQRNQNPQGLKLERECAMCLSEEKTVIFL 793

Query: 1126 PCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKRITARFA 1242
            PC HQ+LCA+CN LHEK GM DCP+CR  I  R  ARFA
Sbjct: 794  PCAHQLLCAKCNELHEKKGMIDCPACRAPIMLRFNARFA 832


>gb|POE64780.1| putative e3 ubiquitin-protein ligase [Quercus suber]
          Length = 868

 Score =  238 bits (608), Expect = 5e-66
 Identities = 144/399 (36%), Positives = 210/399 (52%), Gaps = 14/399 (3%)
 Frame = +1

Query: 88   KLNLSIEKVQSACESSSSVNNKNPLPLKETNSEPVSQEPLKTAEYYLSGIPFDEAKGEHV 267
            K+  ++   ++A  S+SS       P  + N+    Q P    +YY +GIP+D++ G++V
Sbjct: 473  KIPSTLPATENAPTSTSSTAEMKSNPKAQVNTPKAPQIP----DYY-AGIPYDKSLGKYV 527

Query: 268  AQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXX 447
             QD+KDE++L     LQ LQ EL+ W +WAN KVMQ A RL  D+               
Sbjct: 528  PQDEKDELVLKLVPRLQELQDELQCWTEWANQKVMQAAHRLGKDQAELKALRLEKEEAEE 587

Query: 448  XXXXX-----------SETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLT 594
                            SE   AL               RLE +NSVLK++ E A+ R+  
Sbjct: 588  KKKEKKILEENTTKRLSEMENALGNATAEVEIADSTLRRLEVQNSVLKEELEAAKLRASE 647

Query: 595  EAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIR 774
             A S +E +++EQ  ++K                     V   Q++  KAK L  +TE R
Sbjct: 648  SAASYQETVEREQATLKKVQSLEGQKAALQEELGTKKEMVAELQKESGKAKNLYNKTEAR 707

Query: 775  LAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDA 954
              +E+ E      +A SIR ERE LEA AKAEE++  +KAE+E+++Y+ EIK+L++++  
Sbjct: 708  WKQERMEKENLLAQAASIRQERERLEAQAKAEEEMIKQKAESEVQKYKEEIKKLEQKLSQ 767

Query: 955  IKSEAETKKIAAMRKEMSWQPAELTSSQSEVKHGTKSN---KKDRECVMCLTEEMTVVFV 1125
            +K EA++  IAA+R       +   SS  +     ++    K +REC MCL+EE TV+F+
Sbjct: 768  MKMEADSSAIAALRSGTEASSSSTGSSGVQAMQRNQNPQGLKLERECAMCLSEEKTVIFL 827

Query: 1126 PCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKRITARFA 1242
            PC HQ+LCA+CN LHEK GM DCP+CR  I  R  ARFA
Sbjct: 828  PCAHQLLCAKCNELHEKKGMIDCPACRAPIMLRFNARFA 866


>ref|XP_023889470.1| putative E3 ubiquitin-protein ligase RF298 isoform X1 [Quercus suber]
          Length = 885

 Score =  238 bits (608), Expect = 6e-66
 Identities = 144/399 (36%), Positives = 210/399 (52%), Gaps = 14/399 (3%)
 Frame = +1

Query: 88   KLNLSIEKVQSACESSSSVNNKNPLPLKETNSEPVSQEPLKTAEYYLSGIPFDEAKGEHV 267
            K+  ++   ++A  S+SS       P  + N+    Q P    +YY +GIP+D++ G++V
Sbjct: 490  KIPSTLPATENAPTSTSSTAEMKSNPKAQVNTPKAPQIP----DYY-AGIPYDKSLGKYV 544

Query: 268  AQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXX 447
             QD+KDE++L     LQ LQ EL+ W +WAN KVMQ A RL  D+               
Sbjct: 545  PQDEKDELVLKLVPRLQELQDELQCWTEWANQKVMQAAHRLGKDQAELKALRLEKEEAEE 604

Query: 448  XXXXX-----------SETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLT 594
                            SE   AL               RLE +NSVLK++ E A+ R+  
Sbjct: 605  KKKEKKILEENTTKRLSEMENALGNATAEVEIADSTLRRLEVQNSVLKEELEAAKLRASE 664

Query: 595  EAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIR 774
             A S +E +++EQ  ++K                     V   Q++  KAK L  +TE R
Sbjct: 665  SAASYQETVEREQATLKKVQSLEGQKAALQEELGTKKEMVAELQKESGKAKNLYNKTEAR 724

Query: 775  LAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDA 954
              +E+ E      +A SIR ERE LEA AKAEE++  +KAE+E+++Y+ EIK+L++++  
Sbjct: 725  WKQERMEKENLLAQAASIRQERERLEAQAKAEEEMIKQKAESEVQKYKEEIKKLEQKLSQ 784

Query: 955  IKSEAETKKIAAMRKEMSWQPAELTSSQSEVKHGTKSN---KKDRECVMCLTEEMTVVFV 1125
            +K EA++  IAA+R       +   SS  +     ++    K +REC MCL+EE TV+F+
Sbjct: 785  MKMEADSSAIAALRSGTEASSSSTGSSGVQAMQRNQNPQGLKLERECAMCLSEEKTVIFL 844

Query: 1126 PCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKRITARFA 1242
            PC HQ+LCA+CN LHEK GM DCP+CR  I  R  ARFA
Sbjct: 845  PCAHQLLCAKCNELHEKKGMIDCPACRAPIMLRFNARFA 883


>ref|XP_004290694.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Fragaria vesca
            subsp. vesca]
          Length = 827

 Score =  236 bits (601), Expect = 3e-65
 Identities = 141/386 (36%), Positives = 204/386 (52%), Gaps = 28/386 (7%)
 Frame = +1

Query: 172  ETNSEPVSQEPLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDD 351
            ET +   +  P KT +Y  +GIP+DE  G++V QD KDE+I+     LQ LQ EL+ W +
Sbjct: 442  ETKTPVCTSVPPKTVDY-CAGIPYDETLGKYVPQDKKDELIMKLVPQLQKLQNELQSWTE 500

Query: 352  WANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXX-----------SETTAALNTXXX 498
            WAN KVMQV++RL  +RP                              SE   ALN    
Sbjct: 501  WANEKVMQVSRRLGKERPELKALRQEKEEAGKFEKEKQMLDENTMKRLSEMEHALNNASS 560

Query: 499  XXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXX 678
                      RL+  NS LK+    A+  S+  A +L+EAL++E++A++K          
Sbjct: 561  QVDGANATISRLQENNSTLKKQLSSAKILSIESAANLREALEREKKALKKVQAWEGQKSS 620

Query: 679  XXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEAL 858
                       V   Q+DL KAK  L + E R  +  +E      +A S+R+ERE LEAL
Sbjct: 621  LKEELETKKNKVAVLQQDLGKAKNTLQQIEARWKRVIREKEIILAQAASMRNEREQLEAL 680

Query: 859  AKAEEQITAEKAENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSWQ-------- 1014
               EE     KAE+++++Y  EIK L+R++  ++ E+++ +IAA+R++ +          
Sbjct: 681  TLVEEAKMKLKAEDDMQKYVEEIKLLERKLSELQIESDSSRIAALRRDATGSFGAGLADR 740

Query: 1015 ---------PAELTSSQSEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVL 1167
                      +   S +     GT+S ++DRECVMCL+EEM+VVF+PC HQVLCA CN L
Sbjct: 741  KNVVGTKANQSSTVSKKLTNSRGTESLRQDRECVMCLSEEMSVVFLPCAHQVLCATCNEL 800

Query: 1168 HEKNGMKDCPSCRTQIQKRITARFAK 1245
            H K GMKDCPSCR  IQ+RI  ++A+
Sbjct: 801  HLKQGMKDCPSCRALIQRRIKVQYAR 826


>ref|XP_008360889.2| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X3
            [Malus domestica]
          Length = 726

 Score =  234 bits (596), Expect = 3e-65
 Identities = 134/366 (36%), Positives = 202/366 (55%), Gaps = 26/366 (7%)
 Frame = +1

Query: 223  YLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDR 402
            Y +GIP+D++ G++VAQD+KDE+IL     L+ LQ EL+ W +WAN K+MQV++RLS ++
Sbjct: 360  YRAGIPYDKSLGKYVAQDEKDELILKLVPRLEELQNELESWTNWANEKIMQVSRRLSKEQ 419

Query: 403  PXXXXXXXXXXXXXXXXXXX-----------SETTAALNTXXXXXXXXXXXXFRLEYENS 549
            P                              SE   ALN              RLE ENS
Sbjct: 420  PELKILRQEKAEAEKFKKEKQMLEENTVKRISELENALNNATDQVEKATSNICRLELENS 479

Query: 550  VLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXXXXXXVVPKQR 729
             LK++R  AE  ++      +EAL++EQ A++K                     V   Q+
Sbjct: 480  TLKEERSTAERMAIDSTARHQEALEREQNALKKVQAWEGQRSALQEELETGKKKVAALQQ 539

Query: 730  DLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELK 909
            DL KAK +  + E R  +EK E  K   +A+ IR ERE  +AL K EE      AE +++
Sbjct: 540  DLAKAKNVHHQIEARWKREKIEREKILVQADFIRKEREQRQALTKLEEDRIKLSAEYDMR 599

Query: 910  EYESEIKRLQREIDAIKSEAETKKIAAMRK----EMSWQPAELTSSQS-----------E 1044
            EY  +I++++ ++  +K ++++ +IAA+R+         P++  S ++           +
Sbjct: 600  EYMEDIRKVESKLSELKLKSDSSRIAALRRGAAGSFGGSPSDRKSGKAXKGNQNFTSLKK 659

Query: 1045 VKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKR 1224
            VK+ ++  ++DRECVMCL+EEM+VVF+PC HQV+CA CN LH+K GMKDCPSCRT IQ+R
Sbjct: 660  VKN-SQDLRQDRECVMCLSEEMSVVFLPCAHQVVCANCNELHKKQGMKDCPSCRTPIQRR 718

Query: 1225 ITARFA 1242
            I  ++A
Sbjct: 719  INVQYA 724


>ref|XP_008360888.2| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X4
            [Malus domestica]
          Length = 731

 Score =  234 bits (596), Expect = 3e-65
 Identities = 134/366 (36%), Positives = 202/366 (55%), Gaps = 26/366 (7%)
 Frame = +1

Query: 223  YLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDR 402
            Y +GIP+D++ G++VAQD+KDE+IL     L+ LQ EL+ W +WAN K+MQV++RLS ++
Sbjct: 365  YRAGIPYDKSLGKYVAQDEKDELILKLVPRLEELQNELESWTNWANEKIMQVSRRLSKEQ 424

Query: 403  PXXXXXXXXXXXXXXXXXXX-----------SETTAALNTXXXXXXXXXXXXFRLEYENS 549
            P                              SE   ALN              RLE ENS
Sbjct: 425  PELKILRQEKAEAEKFKKEKQMLEENTVKRISELENALNNATDQVEKATSNICRLELENS 484

Query: 550  VLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXXXXXXVVPKQR 729
             LK++R  AE  ++      +EAL++EQ A++K                     V   Q+
Sbjct: 485  TLKEERSTAERMAIDSTARHQEALEREQNALKKVQAWEGQRSALQEELETGKKKVAALQQ 544

Query: 730  DLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELK 909
            DL KAK +  + E R  +EK E  K   +A+ IR ERE  +AL K EE      AE +++
Sbjct: 545  DLAKAKNVHHQIEARWKREKIEREKILVQADFIRKEREQRQALTKLEEDRIKLSAEYDMR 604

Query: 910  EYESEIKRLQREIDAIKSEAETKKIAAMRK----EMSWQPAELTSSQS-----------E 1044
            EY  +I++++ ++  +K ++++ +IAA+R+         P++  S ++           +
Sbjct: 605  EYMEDIRKVESKLSELKLKSDSSRIAALRRGAAGSFGGSPSDRKSGKAXKGNQNFTSLKK 664

Query: 1045 VKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKR 1224
            VK+ ++  ++DRECVMCL+EEM+VVF+PC HQV+CA CN LH+K GMKDCPSCRT IQ+R
Sbjct: 665  VKN-SQDLRQDRECVMCLSEEMSVVFLPCAHQVVCANCNELHKKQGMKDCPSCRTPIQRR 723

Query: 1225 ITARFA 1242
            I  ++A
Sbjct: 724  INVQYA 729


>ref|XP_021803473.1| putative E3 ubiquitin-protein ligase RF298 [Prunus avium]
          Length = 547

 Score =  229 bits (584), Expect = 5e-65
 Identities = 137/371 (36%), Positives = 196/371 (52%), Gaps = 31/371 (8%)
 Frame = +1

Query: 223  YLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDR 402
            Y +GIP+D++ G++V QD+KDE+IL     L+ LQ EL+ W  WAN KVMQV++RLS +R
Sbjct: 175  YCAGIPYDKSIGKYVPQDEKDELILKLVPRLEELQNELQGWTKWANEKVMQVSRRLSKER 234

Query: 403  PXXXXXXXXXXXXXXXXXXX-----------SETTAALNTXXXXXXXXXXXXFRLEYENS 549
            P                              SE   ALN              RLE ENS
Sbjct: 235  PELKMLRQEKEEAEQFKKEKLMLEENTMKRLSELEHALNNATGQVERAKSNICRLEVENS 294

Query: 550  VLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXXXXXXVVPKQR 729
             LK+    A+  +L +A+   +AL +EQ ++++                     V   Q+
Sbjct: 295  TLKKKLLAAKKIALEKARRHLDALDQEQRSLKRAQAWEGEISSLQEELETEKKKVASLQQ 354

Query: 730  DLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELK 909
            DL KAK +  + E R   E  E  K    A SIR+ERE  EALAK EE    +KAEN+++
Sbjct: 355  DLGKAKSVHHQIEARWKWENTENEKLLAHANSIRNEREQREALAKVEEDRIKQKAENDMQ 414

Query: 910  EYESEIKRLQREIDAIKSEAETKKIAAMRKEMSWQPAELTSSQ----------------- 1038
            +Y  +IK L+ ++   + ++++ +IAA+R+  +       S +                 
Sbjct: 415  KYMEDIKELESKLSEFQLKSDSSRIAALRRGAAGSFGGCLSDRKTVMATKGTQNFTSIKR 474

Query: 1039 ---SEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRT 1209
               SE   GT S ++DRECVMCL+EEM+VVF+PC HQV+CA+CN LH+K GM+DCPSCRT
Sbjct: 475  VMNSEDYFGTGSLRQDRECVMCLSEEMSVVFLPCAHQVVCAKCNELHKKQGMEDCPSCRT 534

Query: 1210 QIQKRITARFA 1242
             IQ+RI  ++A
Sbjct: 535  PIQRRINVQYA 545


>ref|XP_021281192.1| putative E3 ubiquitin-protein ligase RF298 [Herrania umbratica]
 ref|XP_021281193.1| putative E3 ubiquitin-protein ligase RF298 [Herrania umbratica]
          Length = 854

 Score =  234 bits (597), Expect = 1e-64
 Identities = 137/371 (36%), Positives = 198/371 (53%), Gaps = 25/371 (6%)
 Frame = +1

Query: 208  KTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKR 387
            KT +YY +GIP+D++ G+H+ QD+KDE+IL     +Q LQ EL  W  W N KVMQ A+R
Sbjct: 484  KTPDYY-AGIPYDQSLGKHIPQDEKDELILKLVPRVQELQNELHSWTQWTNQKVMQAARR 542

Query: 388  LSYDRPXXXXXXXXXXXXXXXXXXX-----------SETTAALNTXXXXXXXXXXXXFRL 534
            LS ++P                              +E   ALN              +L
Sbjct: 543  LSKEQPELKALRQEKEEAEQLQKEKQTMEENTVKRVAEMEGALNDATTQVEDANSTVQKL 602

Query: 535  EYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXXXXXXV 714
            E E+S+LK++ E A+ + +  A S +EA ++EQ+A++                       
Sbjct: 603  EMEHSMLKKEMEVAKLQVVESAASCQEAFEREQKALKDVRSWDGQRSLLQEELALEKQMA 662

Query: 715  VPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKA 894
               Q+ + KAK +  +TE+R  +E+    KF  +A SIR ERE LEA AK EE    +KA
Sbjct: 663  AELQKKVGKAKNVYNQTEMRWKQERLAKEKFLAQAASIRMEREGLEAAAKVEEDKIKQKA 722

Query: 895  ENELKEYESEIKRLQREIDAIKSEAETKKIAAMRKEMSWQPAELTSSQS----------- 1041
            E ++ +YE EIK L+ ++  +K + ++ KIA +R+       + +S              
Sbjct: 723  EKDVHKYEEEIKELENKLSELKMKLDSSKIALLRRGSDGGNGQCSSVNERNQVPSFFERV 782

Query: 1042 -EVKH--GTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQ 1212
             ++K   G++  K++RECVMCL+EE  VVF+PC HQVLC +CN LHEK GMKDCP+CRT 
Sbjct: 783  VDIKDYSGSRGLKQERECVMCLSEEKIVVFLPCAHQVLCVKCNELHEKQGMKDCPACRTL 842

Query: 1213 IQKRITARFAK 1245
            I  RI ARFAK
Sbjct: 843  IDWRICARFAK 853


>ref|XP_017184827.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Malus domestica]
          Length = 837

 Score =  234 bits (596), Expect = 2e-64
 Identities = 134/366 (36%), Positives = 202/366 (55%), Gaps = 26/366 (7%)
 Frame = +1

Query: 223  YLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDR 402
            Y +GIP+D++ G++VAQD+KDE+IL     L+ LQ EL+ W +WAN K+MQV++RLS ++
Sbjct: 471  YRAGIPYDKSLGKYVAQDEKDELILKLVPRLEELQNELESWTNWANEKIMQVSRRLSKEQ 530

Query: 403  PXXXXXXXXXXXXXXXXXXX-----------SETTAALNTXXXXXXXXXXXXFRLEYENS 549
            P                              SE   ALN              RLE ENS
Sbjct: 531  PELKILRQEKAEAEKFKKEKQMLEENTVKRISELENALNNATDQVEKATSNICRLELENS 590

Query: 550  VLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCXXXXXXXXXXXXXXXXXXXXVVPKQR 729
             LK++R  AE  ++      +EAL++EQ A++K                     V   Q+
Sbjct: 591  TLKEERSTAERMAIDSTARHQEALEREQNALKKVQAWEGQRSALQEELETGKKKVAALQQ 650

Query: 730  DLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELK 909
            DL KAK +  + E R  +EK E  K   +A+ IR ERE  +AL K EE      AE +++
Sbjct: 651  DLAKAKNVHHQIEARWKREKIEREKILVQADFIRKEREQRQALTKLEEDRIKLSAEYDMR 710

Query: 910  EYESEIKRLQREIDAIKSEAETKKIAAMRK----EMSWQPAELTSSQS-----------E 1044
            EY  +I++++ ++  +K ++++ +IAA+R+         P++  S ++           +
Sbjct: 711  EYMEDIRKVESKLSELKLKSDSSRIAALRRGAAGSFGGSPSDRKSGKAXKGNQNFTSLKK 770

Query: 1045 VKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKNGMKDCPSCRTQIQKR 1224
            VK+ ++  ++DRECVMCL+EEM+VVF+PC HQV+CA CN LH+K GMKDCPSCRT IQ+R
Sbjct: 771  VKN-SQDLRQDRECVMCLSEEMSVVFLPCAHQVVCANCNELHKKQGMKDCPSCRTPIQRR 829

Query: 1225 ITARFA 1242
            I  ++A
Sbjct: 830  INVQYA 835