BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum22_contig00000676
         (2579 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_021981773.1| isoamylase 3, chloroplastic isoform X1 [Heli...  1099   0.0  
ref|XP_021981774.1| isoamylase 3, chloroplastic isoform X2 [Heli...  1087   0.0  
ref|XP_023763204.1| isoamylase 3, chloroplastic [Lactuca sativa]     1079   0.0  
gb|KVI06832.1| Glycoside hydrolase, catalytic domain-containing ...  1036   0.0  
ref|XP_008342522.1| PREDICTED: isoamylase 3, chloroplastic-like ...  1014   0.0  
ref|XP_009373283.1| PREDICTED: isoamylase 3, chloroplastic isofo...  1001   0.0  
ref|XP_008219350.1| PREDICTED: isoamylase 3, chloroplastic [Prun...  1001   0.0  
ref|XP_020426543.1| isoamylase 3, chloroplastic [Prunus persica]...  1001   0.0  
ref|XP_009373275.1| PREDICTED: isoamylase 3, chloroplastic isofo...  1001   0.0  
ref|XP_008373106.1| PREDICTED: isoamylase 3, chloroplastic-like ...   994   0.0  
ref|XP_021808237.1| isoamylase 3, chloroplastic isoform X4 [Prun...   991   0.0  
ref|XP_021808236.1| isoamylase 3, chloroplastic isoform X3 [Prun...   991   0.0  
ref|NP_001275220.1| isoamylase isoform 3 [Solanum tuberosum] >gi...   986   0.0  
ref|XP_010664352.1| PREDICTED: isoamylase 3, chloroplastic isofo...   985   0.0  
ref|XP_010664351.1| PREDICTED: isoamylase 3, chloroplastic isofo...   985   0.0  
emb|CBI19205.3| unnamed protein product, partial [Vitis vinifera]     985   0.0  
ref|XP_021808234.1| isoamylase 3, chloroplastic isoform X1 [Prun...   984   0.0  
ref|XP_021808235.1| isoamylase 3, chloroplastic isoform X2 [Prun...   984   0.0  
ref|XP_023872402.1| isoamylase 3, chloroplastic [Quercus suber]       984   0.0  
gb|AOQ26243.1| ISA3 [Actinidia deliciosa]                             982   0.0  

>ref|XP_021981773.1| isoamylase 3, chloroplastic isoform X1 [Helianthus annuus]
 gb|OTG14395.1| putative isoamylase 3 [Helianthus annuus]
          Length = 771

 Score = 1099 bits (2843), Expect = 0.0
 Identities = 543/678 (80%), Positives = 574/678 (84%), Gaps = 18/678 (2%)
 Frame = -1

Query: 2354 MILNTTTNS-------------PIPLSAFRANRVILSRNAXXXXXXXXXXXKVIGSECMK 2214
            MILN T +S              + L A  +  VI SRNA           KV GSE M 
Sbjct: 1    MILNATNSSFCDSSLKWSNVSRSLHLPATASMGVIWSRNASGKSFVSGYSGKVKGSEQMN 60

Query: 2213 HKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQHATS 2034
             K AY  S+            VMSE  PSLKSIPGLPNPLGV ET+NGINFALFS+HATS
Sbjct: 61   RKSAYAISKHAEEIVIEDEAAVMSETAPSLKSIPGLPNPLGVLETDNGINFALFSKHATS 120

Query: 2033 VTLCLSLPGRE-----NDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQGS 1869
            VTLCLSLP RE     ND M+ELKLDP +NKTGDIWHICVQ+LPRSNVIYGY+IDGPQ  
Sbjct: 121  VTLCLSLPDREIDASSNDEMIELKLDPIANKTGDIWHICVQELPRSNVIYGYKIDGPQNW 180

Query: 1868 HQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNIPE 1689
            HQGHRFD+SVVLIDPYAKLIEGRR+FGD T+RFSKL GTYDFSS PFDWG+N+AQPNIPE
Sbjct: 181  HQGHRFDSSVVLIDPYAKLIEGRRIFGDPTDRFSKLSGTYDFSSLPFDWGDNYAQPNIPE 240

Query: 1688 KDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEFEF 1509
            KDLV+YEMNVRAFTADKSSGLE+ IRGSY G+IDKIPHLLELG+NAVELLPVFEFDEFEF
Sbjct: 241  KDLVIYEMNVRAFTADKSSGLEQSIRGSYDGVIDKIPHLLELGVNAVELLPVFEFDEFEF 300

Query: 1508 QRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVILDV 1329
            QR PNPRDHM+NTWGYSTINFFAPM RYAS GGGPI ASQEFKEMVKALHLAGIEVILDV
Sbjct: 301  QRHPNPRDHMVNTWGYSTINFFAPMARYASDGGGPIVASQEFKEMVKALHLAGIEVILDV 360

Query: 1328 VYNHTNEADDNNPYTTSFRGIDNKTYYMVDGNGQLLNFSGCGNTLNCNHPVVMELILDSL 1149
            VYNHTNEADD +PYTTSFRGIDNKTYYMVD NGQLLNFSGCGNTLNCNHPVVMELILDSL
Sbjct: 361  VYNHTNEADDKHPYTTSFRGIDNKTYYMVDDNGQLLNFSGCGNTLNCNHPVVMELILDSL 420

Query: 1148 RHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDCGGLY 969
            RHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPL++AIAKDS LSR KII+EPWDCGGLY
Sbjct: 421  RHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLIRAIAKDSTLSRCKIISEPWDCGGLY 480

Query: 968  LVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYHGVNF 789
            LVGKFPNWDRWAEWNG+YRDD+RRFIKGD GMKG FATRVAGS+DLY VNKRKPYHG+NF
Sbjct: 481  LVGKFPNWDRWAEWNGMYRDDIRRFIKGDIGMKGRFATRVAGSADLYKVNKRKPYHGINF 540

Query: 788  VIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFRQMKN 609
            VIAHDGFTLYDLVSYN KH              NLSWNCG EGETSDVNIKALRFRQMKN
Sbjct: 541  VIAHDGFTLYDLVSYNNKHNDANGENGNDGSNDNLSWNCGCEGETSDVNIKALRFRQMKN 600

Query: 608  FHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFFAEVI 429
            FHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKND FRFFAEVI
Sbjct: 601  FHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDLFRFFAEVI 660

Query: 428  KFRLKHRVFRQEIFXXHR 375
            K R KH+VFRQE F  ++
Sbjct: 661  KLRQKHQVFRQEYFIGNK 678



 Score =  144 bits (362), Expect = 1e-31
 Identities = 67/83 (80%), Positives = 74/83 (89%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S FIAFTLHD DEGD+YLAFNAH+YF+KV IPSPPQ R WFRVVDTNLESPNDI+P
Sbjct: 688 DNHESKFIAFTLHDSDEGDLYLAFNAHNYFVKVPIPSPPQGRCWFRVVDTNLESPNDIIP 747

Query: 220 QGVVGIGDTYNVAPYSSILLQAK 152
           +GV GIG+TY+VAPYSSILLQAK
Sbjct: 748 EGVTGIGNTYDVAPYSSILLQAK 770


>ref|XP_021981774.1| isoamylase 3, chloroplastic isoform X2 [Helianthus annuus]
          Length = 713

 Score = 1087 bits (2812), Expect = 0.0
 Identities = 524/620 (84%), Positives = 552/620 (89%), Gaps = 5/620 (0%)
 Frame = -1

Query: 2219 MKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQHA 2040
            M  K AY  S+            VMSE  PSLKSIPGLPNPLGV ET+NGINFALFS+HA
Sbjct: 1    MNRKSAYAISKHAEEIVIEDEAAVMSETAPSLKSIPGLPNPLGVLETDNGINFALFSKHA 60

Query: 2039 TSVTLCLSLPGRE-----NDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQ 1875
            TSVTLCLSLP RE     ND M+ELKLDP +NKTGDIWHICVQ+LPRSNVIYGY+IDGPQ
Sbjct: 61   TSVTLCLSLPDREIDASSNDEMIELKLDPIANKTGDIWHICVQELPRSNVIYGYKIDGPQ 120

Query: 1874 GSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNI 1695
              HQGHRFD+SVVLIDPYAKLIEGRR+FGD T+RFSKL GTYDFSS PFDWG+N+AQPNI
Sbjct: 121  NWHQGHRFDSSVVLIDPYAKLIEGRRIFGDPTDRFSKLSGTYDFSSLPFDWGDNYAQPNI 180

Query: 1694 PEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEF 1515
            PEKDLV+YEMNVRAFTADKSSGLE+ IRGSY G+IDKIPHLLELG+NAVELLPVFEFDEF
Sbjct: 181  PEKDLVIYEMNVRAFTADKSSGLEQSIRGSYDGVIDKIPHLLELGVNAVELLPVFEFDEF 240

Query: 1514 EFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVIL 1335
            EFQR PNPRDHM+NTWGYSTINFFAPM RYAS GGGPI ASQEFKEMVKALHLAGIEVIL
Sbjct: 241  EFQRHPNPRDHMVNTWGYSTINFFAPMARYASDGGGPIVASQEFKEMVKALHLAGIEVIL 300

Query: 1334 DVVYNHTNEADDNNPYTTSFRGIDNKTYYMVDGNGQLLNFSGCGNTLNCNHPVVMELILD 1155
            DVVYNHTNEADD +PYTTSFRGIDNKTYYMVD NGQLLNFSGCGNTLNCNHPVVMELILD
Sbjct: 301  DVVYNHTNEADDKHPYTTSFRGIDNKTYYMVDDNGQLLNFSGCGNTLNCNHPVVMELILD 360

Query: 1154 SLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDCGG 975
            SLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPL++AIAKDS LSR KII+EPWDCGG
Sbjct: 361  SLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLIRAIAKDSTLSRCKIISEPWDCGG 420

Query: 974  LYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYHGV 795
            LYLVGKFPNWDRWAEWNG+YRDD+RRFIKGD GMKG FATRVAGS+DLY VNKRKPYHG+
Sbjct: 421  LYLVGKFPNWDRWAEWNGMYRDDIRRFIKGDIGMKGRFATRVAGSADLYKVNKRKPYHGI 480

Query: 794  NFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFRQM 615
            NFVIAHDGFTLYDLVSYN KH              NLSWNCG EGETSDVNIKALRFRQM
Sbjct: 481  NFVIAHDGFTLYDLVSYNNKHNDANGENGNDGSNDNLSWNCGCEGETSDVNIKALRFRQM 540

Query: 614  KNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFFAE 435
            KNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKND FRFFAE
Sbjct: 541  KNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDLFRFFAE 600

Query: 434  VIKFRLKHRVFRQEIFXXHR 375
            VIK R KH+VFRQE F  ++
Sbjct: 601  VIKLRQKHQVFRQEYFIGNK 620



 Score =  144 bits (362), Expect = 9e-32
 Identities = 67/83 (80%), Positives = 74/83 (89%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S FIAFTLHD DEGD+YLAFNAH+YF+KV IPSPPQ R WFRVVDTNLESPNDI+P
Sbjct: 630 DNHESKFIAFTLHDSDEGDLYLAFNAHNYFVKVPIPSPPQGRCWFRVVDTNLESPNDIIP 689

Query: 220 QGVVGIGDTYNVAPYSSILLQAK 152
           +GV GIG+TY+VAPYSSILLQAK
Sbjct: 690 EGVTGIGNTYDVAPYSSILLQAK 712


>ref|XP_023763204.1| isoamylase 3, chloroplastic [Lactuca sativa]
          Length = 782

 Score = 1079 bits (2790), Expect = 0.0
 Identities = 528/678 (77%), Positives = 571/678 (84%), Gaps = 22/678 (3%)
 Frame = -1

Query: 2354 MILNTTTNSP-------------IPLSAFRANR---VILSRNAXXXXXXXXXXXKVIGSE 2223
            MIL TTT+S              +PLSAFRA     V+ SRNA           KV GSE
Sbjct: 8    MILRTTTSSLCDSSSKISNVNRFLPLSAFRATATMGVMWSRNASGKSSVSGFSSKVKGSE 67

Query: 2222 CMKHKPAYVHSQRXXXXXXXXXXXV-MSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
             +KH   YVH+Q            V MSE   S +SIPG+PNPLGVS+TENGINF+LFS+
Sbjct: 68   PIKHTSTYVHNQHAKEIVIEEEEVVVMSETDSSFESIPGVPNPLGVSKTENGINFSLFSK 127

Query: 2045 HATSVTLCLSLPGRENDG-----MVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDG 1881
            HATSVTLCLSL    ND      M+ELKLDP  NKTGDIWHICV +LP  NV+YGY+I+G
Sbjct: 128  HATSVTLCLSLQDSGNDASPNDEMIELKLDPHVNKTGDIWHICVLELPLRNVVYGYKING 187

Query: 1880 PQGSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQP 1701
            PQG H+GHRFDN+VVLIDPYAKLI+GR++FGD   +FSK +GTYDFSS PFDWG+N+  P
Sbjct: 188  PQGFHEGHRFDNTVVLIDPYAKLIQGRKIFGDVKNKFSKFYGTYDFSSLPFDWGDNYTPP 247

Query: 1700 NIPEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFD 1521
            NIPEKDLVVYEMNVRAFTADKSSG+EE++RGSYLGII+KIPHLLELGINAVELLPVFEFD
Sbjct: 248  NIPEKDLVVYEMNVRAFTADKSSGVEENLRGSYLGIIEKIPHLLELGINAVELLPVFEFD 307

Query: 1520 EFEFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEV 1341
            EFEFQRRPNPRDHMINTWGYSTINFF+PMTRYAS GGGPI A  EFK+MVKALHLAGIEV
Sbjct: 308  EFEFQRRPNPRDHMINTWGYSTINFFSPMTRYASGGGGPIAARLEFKQMVKALHLAGIEV 367

Query: 1340 ILDVVYNHTNEADDNNPYTTSFRGIDNKTYYMVDGNGQLLNFSGCGNTLNCNHPVVMELI 1161
            ILDVVYNHTNEADD  PYTTSFRGIDNK YYMVDGNG+LLNFSGCGNTLNCNHPVVMELI
Sbjct: 368  ILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMVDGNGELLNFSGCGNTLNCNHPVVMELI 427

Query: 1160 LDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDC 981
            LDSLRHWV EYHVDGFRFDLASVLCRGTDGSP+DAPPL++AIAKDSILSR KIIAEPWDC
Sbjct: 428  LDSLRHWVIEYHVDGFRFDLASVLCRGTDGSPVDAPPLIRAIAKDSILSRCKIIAEPWDC 487

Query: 980  GGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYH 801
            GGLYLVGKFPNWDRWAEWNGIYRDD+RRFIKGDTGMKG FATR+AGS+DLY VN+RKPYH
Sbjct: 488  GGLYLVGKFPNWDRWAEWNGIYRDDMRRFIKGDTGMKGAFATRIAGSADLYKVNQRKPYH 547

Query: 800  GVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFR 621
            GVNFVIAHDGFTLYDLVSYN+KH              NLSWNCGHEGETSD NIK+LRFR
Sbjct: 548  GVNFVIAHDGFTLYDLVSYNFKHNDANGEGGNDGSNDNLSWNCGHEGETSDANIKSLRFR 607

Query: 620  QMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFF 441
            QMKNFH+ALMVSQGVPMMLMGDEYGHTR GNNNSYGHDT +NHFQWGQLDARKNDCFRFF
Sbjct: 608  QMKNFHMALMVSQGVPMMLMGDEYGHTRYGNNNSYGHDTTLNHFQWGQLDARKNDCFRFF 667

Query: 440  AEVIKFRLKHRVFRQEIF 387
            AEVIKFR K+RVFRQE F
Sbjct: 668  AEVIKFRQKYRVFRQEYF 685



 Score =  154 bits (389), Expect = 6e-35
 Identities = 72/80 (90%), Positives = 76/80 (95%)
 Frame = -2

Query: 388 SXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVPQGVV 209
           S FIAFTLHD DEGDIYLAFNAHDYF++V IPSPP QRRWFRVVDTNLESPND+VP+GVV
Sbjct: 703 SRFIAFTLHDDDEGDIYLAFNAHDYFVQVPIPSPPLQRRWFRVVDTNLESPNDMVPEGVV 762

Query: 208 GIGDTYNVAPYSSILLQAKP 149
           GIGDTYNVAPYSSILLQAKP
Sbjct: 763 GIGDTYNVAPYSSILLQAKP 782


>gb|KVI06832.1| Glycoside hydrolase, catalytic domain-containing protein [Cynara
            cardunculus var. scolymus]
          Length = 725

 Score = 1036 bits (2678), Expect = 0.0
 Identities = 520/673 (77%), Positives = 552/673 (82%), Gaps = 8/673 (1%)
 Frame = -1

Query: 2381 CLPDASLFRMILNTTTNSPIPLSAFRANR---VILSRNAXXXXXXXXXXXKVIGSECMKH 2211
            CLPD+S       +  N  +PLSAF A     VI SRNA           KV G E +KH
Sbjct: 10   CLPDSSS----KFSNVNHSLPLSAFGATDATGVIWSRNASGKSSVSGLSCKVKGLEPVKH 65

Query: 2210 KPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQHATSV 2031
            K   VHSQR           VMSE  P+LK+IPGLPNPLGVSETE GINFALFSQHATSV
Sbjct: 66   KSTCVHSQRAEEIVVEDEVTVMSETSPALKNIPGLPNPLGVSETEKGINFALFSQHATSV 125

Query: 2030 TLCLSLPGR-----ENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQGSH 1866
            TLCL LP R      NDGM+ELKLDP +NKTGDIWHIC+Q+LPRSNVIYGYR+ GPQG H
Sbjct: 126  TLCLLLPERGDDASPNDGMIELKLDPHANKTGDIWHICIQELPRSNVIYGYRVYGPQGWH 185

Query: 1865 QGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNIPEK 1686
            QGHRFDNS VLIDPYAKLIEGRR+FGD+++RFSKL+GTYDFSS PFDWG+N+AQPNIPEK
Sbjct: 186  QGHRFDNSDVLIDPYAKLIEGRRIFGDASDRFSKLYGTYDFSSLPFDWGDNYAQPNIPEK 245

Query: 1685 DLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEFEFQ 1506
            DLVVYEMNVRAFTADKSSGLE+DI GSYLG+I+KIPHLLELGINAVELLP+FEFDEFEFQ
Sbjct: 246  DLVVYEMNVRAFTADKSSGLEQDICGSYLGLIEKIPHLLELGINAVELLPIFEFDEFEFQ 305

Query: 1505 RRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVILDVV 1326
            RRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPI ASQEFKEMVKALHLAGIEVILDVV
Sbjct: 306  RRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPIGASQEFKEMVKALHLAGIEVILDVV 365

Query: 1325 YNHTNEADDNNPYTTSFRGIDNKTYYMVDGNGQLLNFSGCGNTLNCNHPVVMELILDSLR 1146
            YNHTNEADD NPYTTSFRGIDNK YYMVDGNGQLLNFSGCGNTLNCNHPVVMELILDSLR
Sbjct: 366  YNHTNEADDKNPYTTSFRGIDNKIYYMVDGNGQLLNFSGCGNTLNCNHPVVMELILDSLR 425

Query: 1145 HWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDCGGLYL 966
            HWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPL++AIAKDSILSR KIIAEPWDCGGLYL
Sbjct: 426  HWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLIRAIAKDSILSRCKIIAEPWDCGGLYL 485

Query: 965  VGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYHGVNFV 786
            VGKFPNWDRWAEWNG+YRDD+RRFIKGDTGMKGGFATRVAGS+DLY VNKRKPYHGVNF+
Sbjct: 486  VGKFPNWDRWAEWNGMYRDDMRRFIKGDTGMKGGFATRVAGSADLYKVNKRKPYHGVNFI 545

Query: 785  IAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFRQMKNF 606
            IAHDGFTLYDLVSYNYKH              NLSWNCG+EGETS+ NIKALRFRQMKNF
Sbjct: 546  IAHDGFTLYDLVSYNYKHNDANGEGGNDGSNDNLSWNCGYEGETSEANIKALRFRQMKNF 605

Query: 605  HLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFFAEVIK 426
            HLALMVS                                   QLDARKNDCFRFF+EVIK
Sbjct: 606  HLALMVS-----------------------------------QLDARKNDCFRFFSEVIK 630

Query: 425  FRLKHRVFRQEIF 387
            FR K+RVF QE F
Sbjct: 631  FRKKNRVFSQEYF 643



 Score =  112 bits (281), Expect = 8e-22
 Identities = 58/84 (69%), Positives = 62/84 (73%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S FIAFTLHD DEGD+YLAFNAHDYF+KV               DTNLESP+D+VP
Sbjct: 657 DNYESKFIAFTLHDRDEGDVYLAFNAHDYFVKV---------------DTNLESPHDMVP 701

Query: 220 QGVVGIGDTYNVAPYSSILLQAKP 149
            GV GIGDTYNVAPYSSILLQAKP
Sbjct: 702 DGVDGIGDTYNVAPYSSILLQAKP 725


>ref|XP_008342522.1| PREDICTED: isoamylase 3, chloroplastic-like [Malus domestica]
          Length = 776

 Score = 1014 bits (2623), Expect = 0.0
 Identities = 480/617 (77%), Positives = 532/617 (86%), Gaps = 7/617 (1%)
 Frame = -1

Query: 2216 KHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQHAT 2037
            KH  A  + QR            +SE+  S K+ PG   PLGVSE +NGINFA+FSQHAT
Sbjct: 64   KHSTANAYGQRAQERVLEEEAPQLSESSSSWKTFPGQAFPLGVSEVDNGINFAIFSQHAT 123

Query: 2036 SVTLCLSLPGRE-----NDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQG 1872
            +VTLCLSLP RE     + GM+EL LDP  NKTGDIWHIC++DLPRSNV+YGYR+DGPQG
Sbjct: 124  AVTLCLSLPERERLGGLDGGMMELPLDPNVNKTGDIWHICIKDLPRSNVLYGYRMDGPQG 183

Query: 1871 SHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNIP 1692
             HQGHRFD+S+VL+DPYAKL+EGRR+FGD+T++ SK  GTYDF S PFDWGEN+  PNIP
Sbjct: 184  WHQGHRFDSSIVLVDPYAKLVEGRRMFGDTTKKLSKFLGTYDFDSLPFDWGENYKLPNIP 243

Query: 1691 EKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEFE 1512
            EKDLV+YEMNVRA+TAD+SSGL+ DIRGSYLG+I+KIPHL ELGINAVELLPVFEFDEFE
Sbjct: 244  EKDLVIYEMNVRAYTADESSGLDPDIRGSYLGMIEKIPHLRELGINAVELLPVFEFDEFE 303

Query: 1511 FQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVILD 1332
            FQRRPNPRDHMINTWGYSTINFFAPM+RYASAGGGP+ ASQEFK+MVKALH AGIEVILD
Sbjct: 304  FQRRPNPRDHMINTWGYSTINFFAPMSRYASAGGGPVKASQEFKQMVKALHGAGIEVILD 363

Query: 1331 VVYNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSGCGNTLNCNHPVVMELIL 1158
            VVYNHTNEADD NPYTTSFRGIDNK YYM+D   NGQLLN+SGCGNTLNCNHPVVMELIL
Sbjct: 364  VVYNHTNEADDANPYTTSFRGIDNKVYYMLDMNNNGQLLNYSGCGNTLNCNHPVVMELIL 423

Query: 1157 DSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDCG 978
            DSLRHWV EYHVDGFRFDLASVLCRGTDG+PLDAPP+++AIAKD+ILSR KII+EPWDCG
Sbjct: 424  DSLRHWVVEYHVDGFRFDLASVLCRGTDGTPLDAPPVIRAIAKDAILSRCKIISEPWDCG 483

Query: 977  GLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYHG 798
            GLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV+GS+DLY VNKRKPYH 
Sbjct: 484  GLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRVSGSADLYKVNKRKPYHS 543

Query: 797  VNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFRQ 618
            VNF+IAHDGFTLYDLVSYN+KH              NLSWNCGHEGET D  IKA+R RQ
Sbjct: 544  VNFIIAHDGFTLYDLVSYNFKHNEANGEGGNDGSNDNLSWNCGHEGETDDSTIKAIRSRQ 603

Query: 617  MKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFFA 438
            MKNFHLALM+SQG PMMLMGDEYGHTR+GNNNSYGHDTA+NHFQWGQL+AR N+ FRFF 
Sbjct: 604  MKNFHLALMISQGTPMMLMGDEYGHTRHGNNNSYGHDTALNHFQWGQLEARNNNHFRFFK 663

Query: 437  EVIKFRLKHRVFRQEIF 387
            E IKFR  HRVF +E F
Sbjct: 664  EAIKFRRAHRVFGREAF 680



 Score =  129 bits (323), Expect = 7e-27
 Identities = 61/83 (73%), Positives = 68/83 (81%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S F+AFT H+  EGDIYLAFNAHD FI V IP+PP +RRWFRVVDTNLESPNDIVP
Sbjct: 694 DNYESKFLAFTFHESSEGDIYLAFNAHDCFITVPIPAPPVKRRWFRVVDTNLESPNDIVP 753

Query: 220 QGVVGIGDTYNVAPYSSILLQAK 152
            GV GI  TYN+AP+SS+LLQAK
Sbjct: 754 DGVPGIEGTYNMAPFSSVLLQAK 776


>ref|XP_009373283.1| PREDICTED: isoamylase 3, chloroplastic isoform X2 [Pyrus x
            bretschneideri]
          Length = 773

 Score = 1001 bits (2589), Expect = 0.0
 Identities = 473/614 (77%), Positives = 529/614 (86%), Gaps = 4/614 (0%)
 Frame = -1

Query: 2216 KHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQHAT 2037
            KH  A  + Q             +SE+  S K+ PG   PLGVSE ++GINFA+FS+HAT
Sbjct: 64   KHSTANAYGQHAQERVLEEEAPQVSESSLSWKTFPGQAFPLGVSEVDSGINFAIFSRHAT 123

Query: 2036 SVTLCLSLP---GRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQGSH 1866
            +VTLCLSLP   G  + GM+EL LDP  NKTGDIWH+C++DLPRSNV+YGYR+DGP+G H
Sbjct: 124  AVTLCLSLPEGLGGLDGGMMELPLDPNVNKTGDIWHMCIKDLPRSNVLYGYRMDGPRGWH 183

Query: 1865 QGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNIPEK 1686
            QGHRFD+S+VL+DPYAKL+EGRR+FGD+T++ SK  GTYDF S PFDWGEN+  PNIPEK
Sbjct: 184  QGHRFDSSIVLVDPYAKLVEGRRMFGDTTKKLSKFLGTYDFDSLPFDWGENYKLPNIPEK 243

Query: 1685 DLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEFEFQ 1506
            DLV+YEMNVRA+TAD+SSGL+ DIRGSYLG+I+KIPHL ELGINAVELLPVFEFDEFEFQ
Sbjct: 244  DLVIYEMNVRAYTADESSGLDPDIRGSYLGMIEKIPHLRELGINAVELLPVFEFDEFEFQ 303

Query: 1505 RRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVILDVV 1326
            RRPNPRDHMINTWGYSTINFFAPM+RYASAGGGP+ ASQEFK+MVKALH AGIEVILDVV
Sbjct: 304  RRPNPRDHMINTWGYSTINFFAPMSRYASAGGGPVKASQEFKQMVKALHGAGIEVILDVV 363

Query: 1325 YNHTNEADDNNPYTTSFRGIDNKTYYMVD-GNGQLLNFSGCGNTLNCNHPVVMELILDSL 1149
            YNHTNEADD NPYTTSFRGIDNK YYM+D  NGQLLN+SGCGNTLNCNHPVVMELILDSL
Sbjct: 364  YNHTNEADDANPYTTSFRGIDNKVYYMLDVNNGQLLNYSGCGNTLNCNHPVVMELILDSL 423

Query: 1148 RHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDCGGLY 969
            RHWV EYHVDGFRFDLASVLCRGTDG+PLDAPP+++AIAKD+ILSR KII+EPWDCGGLY
Sbjct: 424  RHWVVEYHVDGFRFDLASVLCRGTDGTPLDAPPVIRAIAKDAILSRCKIISEPWDCGGLY 483

Query: 968  LVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYHGVNF 789
            LVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV+GS+DLY VNKRKPYH VNF
Sbjct: 484  LVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRVSGSADLYKVNKRKPYHSVNF 543

Query: 788  VIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFRQMKN 609
            +IAHDGFTLYDLVSYN+KH              NLSWNCGHEGET D  IKA+R R MKN
Sbjct: 544  IIAHDGFTLYDLVSYNFKHNDANGEGGNDGSNDNLSWNCGHEGETDDSTIKAIRSRPMKN 603

Query: 608  FHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFFAEVI 429
            FHLALM+SQG PMMLMGDEYGHTR+GNNNSYGHDTA+NHFQWGQL+AR N+ FRFF E I
Sbjct: 604  FHLALMISQGTPMMLMGDEYGHTRHGNNNSYGHDTALNHFQWGQLEARNNNHFRFFKEAI 663

Query: 428  KFRLKHRVFRQEIF 387
            KFR  HRVF +E F
Sbjct: 664  KFRRAHRVFGRETF 677



 Score =  132 bits (331), Expect = 8e-28
 Identities = 62/83 (74%), Positives = 69/83 (83%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S F+AFT HD  EGDIYLAFNAHD FI V IP+PP++RRWFRVVDTNLESPNDIVP
Sbjct: 691 DNYESKFLAFTFHDSSEGDIYLAFNAHDCFITVPIPAPPEKRRWFRVVDTNLESPNDIVP 750

Query: 220 QGVVGIGDTYNVAPYSSILLQAK 152
            G+ GI  TYN+AP+SSILLQAK
Sbjct: 751 DGIPGIEGTYNMAPFSSILLQAK 773


>ref|XP_008219350.1| PREDICTED: isoamylase 3, chloroplastic [Prunus mume]
          Length = 773

 Score = 1001 bits (2588), Expect = 0.0
 Identities = 473/618 (76%), Positives = 529/618 (85%), Gaps = 5/618 (0%)
 Frame = -1

Query: 2225 ECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
            +C+KH+ A  + QR            MSE  PS K+ PG   PLGVSE ++G NFA+FSQ
Sbjct: 60   DCVKHRTANAYGQRAQERVLEDEALQMSETSPSWKTFPGQAFPLGVSEVDSGTNFAIFSQ 119

Query: 2045 HATSVTLCLSLP---GRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQ 1875
            HAT+VTLCLSLP   GR + GM+E  LD   NKTGDIWHIC++DLPRSNV+YGYR+DGP+
Sbjct: 120  HATAVTLCLSLPERLGRVDGGMMEFSLDRNENKTGDIWHICIKDLPRSNVLYGYRMDGPR 179

Query: 1874 GSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNI 1695
            G H+GHRFD+ VVLIDPYAKL++GRR FGDS+++FSK  GTYDF S PF WG+N+  PNI
Sbjct: 180  GWHEGHRFDSRVVLIDPYAKLVDGRRFFGDSSKKFSKFLGTYDFDSLPFAWGDNYKLPNI 239

Query: 1694 PEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEF 1515
            PEKDLV+YEMNVRAFTA +SSGL+ DIRGSYLG+I+KIPHL ELGINAVELLPVFEFDEF
Sbjct: 240  PEKDLVIYEMNVRAFTASESSGLDPDIRGSYLGVIEKIPHLQELGINAVELLPVFEFDEF 299

Query: 1514 EFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVIL 1335
            EFQRRPNPRDHMINTWGYSTINFFAPM+RYAS GGGP+ ASQEFK+MVKALH AGIEVIL
Sbjct: 300  EFQRRPNPRDHMINTWGYSTINFFAPMSRYASDGGGPLKASQEFKQMVKALHGAGIEVIL 359

Query: 1334 DVVYNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSGCGNTLNCNHPVVMELI 1161
            DVVYNHTNEADD NPYTTSFRGIDNK YYM+D   NGQLLNFSGCGNTLNCNHPVVMELI
Sbjct: 360  DVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLNNNGQLLNFSGCGNTLNCNHPVVMELI 419

Query: 1160 LDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDC 981
            +DSLRHWVTEYHVDGFRFDLASVLCRG+DGSPL APP+++AIAKDSILSR KII+EPWDC
Sbjct: 420  IDSLRHWVTEYHVDGFRFDLASVLCRGSDGSPLSAPPVIRAIAKDSILSRCKIISEPWDC 479

Query: 980  GGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYH 801
            GGLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV+GS+DLY VNKRKPYH
Sbjct: 480  GGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRVSGSADLYKVNKRKPYH 539

Query: 800  GVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFR 621
             VNFV+AHDGFTLYDLVSYN+KH              NLSWNCG+EGET D  +KA+R R
Sbjct: 540  SVNFVVAHDGFTLYDLVSYNFKHNDANGEGGNDGSSDNLSWNCGYEGETDDSTVKAIRSR 599

Query: 620  QMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFF 441
            QMKNFHLALM+SQG PMMLMGDEYGH+RNGNNNSYGHDTAINHFQWG L+AR ++ FRFF
Sbjct: 600  QMKNFHLALMISQGTPMMLMGDEYGHSRNGNNNSYGHDTAINHFQWGLLEARNSNHFRFF 659

Query: 440  AEVIKFRLKHRVFRQEIF 387
             EVIKFR  HRVF ++ F
Sbjct: 660  KEVIKFRRTHRVFGRDTF 677



 Score =  125 bits (313), Expect = 1e-25
 Identities = 58/79 (73%), Positives = 66/79 (83%)
 Frame = -2

Query: 388 SXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVPQGVV 209
           S F+AFT HD + GD+Y+AFNAHDYFIKV IP+ P +RRWFRVVDTNLESPND V  GV 
Sbjct: 695 SKFLAFTFHDNNGGDVYMAFNAHDYFIKVPIPAAPVKRRWFRVVDTNLESPNDFVLDGVP 754

Query: 208 GIGDTYNVAPYSSILLQAK 152
           GI  TYN+APYSSILL+AK
Sbjct: 755 GIKSTYNMAPYSSILLEAK 773


>ref|XP_020426543.1| isoamylase 3, chloroplastic [Prunus persica]
 gb|ONI35223.1| hypothetical protein PRUPE_1G523500 [Prunus persica]
          Length = 773

 Score = 1001 bits (2587), Expect = 0.0
 Identities = 474/618 (76%), Positives = 529/618 (85%), Gaps = 5/618 (0%)
 Frame = -1

Query: 2225 ECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
            +C+KH+ A V+ QR            MSE  PS K+ PG   PLGVSE ++G NFA+FSQ
Sbjct: 60   DCVKHRTANVYGQRAQERVLEDEAPQMSETSPSWKTFPGQAFPLGVSEVDSGTNFAIFSQ 119

Query: 2045 HATSVTLCLSLP---GRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQ 1875
            HAT+VTLCLSLP   GR + GM+E  LD   NKTGDIWHIC++DLPRSNV+YGYR+DGP+
Sbjct: 120  HATAVTLCLSLPERLGRVDGGMMEFSLDRNVNKTGDIWHICIKDLPRSNVLYGYRMDGPR 179

Query: 1874 GSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNI 1695
            G H+GHRFD+ VVLIDPYAKL++GRR FGDS+++FSK  GTYDF S PF WG+N+  PNI
Sbjct: 180  GWHEGHRFDSRVVLIDPYAKLVDGRRFFGDSSKKFSKFLGTYDFDSLPFAWGDNYKLPNI 239

Query: 1694 PEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEF 1515
            PEKDLV+YEMNVRAFTA +SSGL+ DIRGSYLG+I+KIPHL ELGINAVELLPVFEFDEF
Sbjct: 240  PEKDLVIYEMNVRAFTASESSGLDPDIRGSYLGVIEKIPHLQELGINAVELLPVFEFDEF 299

Query: 1514 EFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVIL 1335
            EFQRRPNPRDHMINTWGYSTINFFAPM+RYAS GGGP+ ASQEFK+MVKALH AGIEVIL
Sbjct: 300  EFQRRPNPRDHMINTWGYSTINFFAPMSRYASDGGGPLKASQEFKQMVKALHGAGIEVIL 359

Query: 1334 DVVYNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSGCGNTLNCNHPVVMELI 1161
            DVVYNHTNEADD NPYTTSFRGIDNK YYM+D   NGQLLNFSGCGNTLNCNHPVVMELI
Sbjct: 360  DVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLNNNGQLLNFSGCGNTLNCNHPVVMELI 419

Query: 1160 LDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDC 981
            +DSLRHWVTEYHVDGFRFDLAS LCRG+DGSPL APP+++AIAKDSILSR KII+EPWDC
Sbjct: 420  IDSLRHWVTEYHVDGFRFDLASALCRGSDGSPLSAPPVIRAIAKDSILSRCKIISEPWDC 479

Query: 980  GGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYH 801
            GGLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV+GS+DLY VNKRKPYH
Sbjct: 480  GGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRVSGSADLYKVNKRKPYH 539

Query: 800  GVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFR 621
             VNFVIAHDGFTLYDLVSYN+KH              NLSWNCG+EGET D  +KA+R R
Sbjct: 540  SVNFVIAHDGFTLYDLVSYNFKHNDANGEGGNDGSNDNLSWNCGYEGETDDSTVKAIRSR 599

Query: 620  QMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFF 441
            QMKNFHLALM+SQG PMMLMGDEYGH+RNGNNNSYGHDTAINHFQWG L+AR ++ FRFF
Sbjct: 600  QMKNFHLALMISQGTPMMLMGDEYGHSRNGNNNSYGHDTAINHFQWGLLEARNSNHFRFF 659

Query: 440  AEVIKFRLKHRVFRQEIF 387
             EVIKFR  HRVF ++ F
Sbjct: 660  KEVIKFRRTHRVFGRDTF 677



 Score =  125 bits (313), Expect = 1e-25
 Identities = 58/79 (73%), Positives = 66/79 (83%)
 Frame = -2

Query: 388 SXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVPQGVV 209
           S F+AFT HD + GD+Y+AFNAHDYFIKV IP+ P +RRWFRVVDTNLESPND V  GV 
Sbjct: 695 SKFLAFTFHDNNGGDVYMAFNAHDYFIKVPIPAAPVKRRWFRVVDTNLESPNDFVLDGVP 754

Query: 208 GIGDTYNVAPYSSILLQAK 152
           GI  TYN+APYSSILL+AK
Sbjct: 755 GIKSTYNMAPYSSILLEAK 773


>ref|XP_009373275.1| PREDICTED: isoamylase 3, chloroplastic isoform X1 [Pyrus x
            bretschneideri]
          Length = 775

 Score = 1001 bits (2587), Expect = 0.0
 Identities = 473/616 (76%), Positives = 529/616 (85%), Gaps = 6/616 (0%)
 Frame = -1

Query: 2216 KHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQHAT 2037
            KH  A  + Q             +SE+  S K+ PG   PLGVSE ++GINFA+FS+HAT
Sbjct: 64   KHSTANAYGQHAQERVLEEEAPQVSESSLSWKTFPGQAFPLGVSEVDSGINFAIFSRHAT 123

Query: 2036 SVTLCLSLPGRE-----NDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQG 1872
            +VTLCLSLP  E     + GM+EL LDP  NKTGDIWH+C++DLPRSNV+YGYR+DGP+G
Sbjct: 124  AVTLCLSLPEGERLGGLDGGMMELPLDPNVNKTGDIWHMCIKDLPRSNVLYGYRMDGPRG 183

Query: 1871 SHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNIP 1692
             HQGHRFD+S+VL+DPYAKL+EGRR+FGD+T++ SK  GTYDF S PFDWGEN+  PNIP
Sbjct: 184  WHQGHRFDSSIVLVDPYAKLVEGRRMFGDTTKKLSKFLGTYDFDSLPFDWGENYKLPNIP 243

Query: 1691 EKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEFE 1512
            EKDLV+YEMNVRA+TAD+SSGL+ DIRGSYLG+I+KIPHL ELGINAVELLPVFEFDEFE
Sbjct: 244  EKDLVIYEMNVRAYTADESSGLDPDIRGSYLGMIEKIPHLRELGINAVELLPVFEFDEFE 303

Query: 1511 FQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVILD 1332
            FQRRPNPRDHMINTWGYSTINFFAPM+RYASAGGGP+ ASQEFK+MVKALH AGIEVILD
Sbjct: 304  FQRRPNPRDHMINTWGYSTINFFAPMSRYASAGGGPVKASQEFKQMVKALHGAGIEVILD 363

Query: 1331 VVYNHTNEADDNNPYTTSFRGIDNKTYYMVD-GNGQLLNFSGCGNTLNCNHPVVMELILD 1155
            VVYNHTNEADD NPYTTSFRGIDNK YYM+D  NGQLLN+SGCGNTLNCNHPVVMELILD
Sbjct: 364  VVYNHTNEADDANPYTTSFRGIDNKVYYMLDVNNGQLLNYSGCGNTLNCNHPVVMELILD 423

Query: 1154 SLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDCGG 975
            SLRHWV EYHVDGFRFDLASVLCRGTDG+PLDAPP+++AIAKD+ILSR KII+EPWDCGG
Sbjct: 424  SLRHWVVEYHVDGFRFDLASVLCRGTDGTPLDAPPVIRAIAKDAILSRCKIISEPWDCGG 483

Query: 974  LYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYHGV 795
            LYLVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV+GS+DLY VNKRKPYH V
Sbjct: 484  LYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRVSGSADLYKVNKRKPYHSV 543

Query: 794  NFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFRQM 615
            NF+IAHDGFTLYDLVSYN+KH              NLSWNCGHEGET D  IKA+R R M
Sbjct: 544  NFIIAHDGFTLYDLVSYNFKHNDANGEGGNDGSNDNLSWNCGHEGETDDSTIKAIRSRPM 603

Query: 614  KNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFFAE 435
            KNFHLALM+SQG PMMLMGDEYGHTR+GNNNSYGHDTA+NHFQWGQL+AR N+ FRFF E
Sbjct: 604  KNFHLALMISQGTPMMLMGDEYGHTRHGNNNSYGHDTALNHFQWGQLEARNNNHFRFFKE 663

Query: 434  VIKFRLKHRVFRQEIF 387
             IKFR  HRVF +E F
Sbjct: 664  AIKFRRAHRVFGRETF 679



 Score =  132 bits (331), Expect = 8e-28
 Identities = 62/83 (74%), Positives = 69/83 (83%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S F+AFT HD  EGDIYLAFNAHD FI V IP+PP++RRWFRVVDTNLESPNDIVP
Sbjct: 693 DNYESKFLAFTFHDSSEGDIYLAFNAHDCFITVPIPAPPEKRRWFRVVDTNLESPNDIVP 752

Query: 220 QGVVGIGDTYNVAPYSSILLQAK 152
            G+ GI  TYN+AP+SSILLQAK
Sbjct: 753 DGIPGIEGTYNMAPFSSILLQAK 775


>ref|XP_008373106.1| PREDICTED: isoamylase 3, chloroplastic-like [Malus domestica]
          Length = 775

 Score =  994 bits (2571), Expect = 0.0
 Identities = 470/615 (76%), Positives = 526/615 (85%), Gaps = 5/615 (0%)
 Frame = -1

Query: 2216 KHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQHAT 2037
            +H  A  + QR            +SE+ PS K  PG   PLGVSE ++GINFA+FSQHAT
Sbjct: 65   RHSTASAYGQRAQERVLEEEATQLSESSPSWKLFPGQAFPLGVSEVDSGINFAIFSQHAT 124

Query: 2036 SVTLCLSLP---GRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQGSH 1866
            +VTLCLS P   G  + GM++L LD   NKTGDIWHIC+QDLPRSNV+YGYR+DGP+G H
Sbjct: 125  AVTLCLSFPERLGGLDGGMMDLPLDRNVNKTGDIWHICIQDLPRSNVLYGYRMDGPRGWH 184

Query: 1865 QGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNIPEK 1686
            QGHRFD+S+VL+DPY KL+EGRR+FGD+T++ SK  GTYDF S PF+WG+N+  PNIPEK
Sbjct: 185  QGHRFDSSIVLVDPYTKLVEGRRIFGDTTKKLSKFLGTYDFDSLPFNWGDNYKLPNIPEK 244

Query: 1685 DLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEFEFQ 1506
            DLV+YEMNVRA+TAD+SSGL+ DIRGSYLG+I KIPHL ELGINAVELLPV EFDEFEFQ
Sbjct: 245  DLVIYEMNVRAYTADESSGLDPDIRGSYLGVIQKIPHLRELGINAVELLPVVEFDEFEFQ 304

Query: 1505 RRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVILDVV 1326
            RR NPRDHMINTWGYSTINFFAPM+RYASAGGGP+ ASQEFK+MVKALH AGIEVILDVV
Sbjct: 305  RRRNPRDHMINTWGYSTINFFAPMSRYASAGGGPLKASQEFKQMVKALHGAGIEVILDVV 364

Query: 1325 YNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSGCGNTLNCNHPVVMELILDS 1152
            YNHTNEADD NPYTTSFRGIDNK YYM+D   NGQLLN+SGCGNTLNCNHPVVMELILDS
Sbjct: 365  YNHTNEADDANPYTTSFRGIDNKVYYMLDVNNNGQLLNYSGCGNTLNCNHPVVMELILDS 424

Query: 1151 LRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDCGGL 972
            LRHWVTEYHVDGFRFDLASVLCRG DG+PLDAPPL++AIAKD+ILSR KII+EPWDCGGL
Sbjct: 425  LRHWVTEYHVDGFRFDLASVLCRGADGTPLDAPPLIRAIAKDAILSRCKIISEPWDCGGL 484

Query: 971  YLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYHGVN 792
            YLVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV+GS+DLY VNKRKPYH VN
Sbjct: 485  YLVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRVSGSADLYKVNKRKPYHSVN 544

Query: 791  FVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFRQMK 612
            F+IAHDGFTLYDLVSYN+KH              NLSWNCGHEGET+D  IKA+R RQMK
Sbjct: 545  FIIAHDGFTLYDLVSYNFKHNDANGEGGNDGNNDNLSWNCGHEGETNDSTIKAIRSRQMK 604

Query: 611  NFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFFAEV 432
            NFHLALM+SQG PMMLMGDEYGHTR+GNNNSYGHDTA+NHFQWG L+ARKN+ FRFF E 
Sbjct: 605  NFHLALMISQGTPMMLMGDEYGHTRHGNNNSYGHDTALNHFQWGLLEARKNNHFRFFKEA 664

Query: 431  IKFRLKHRVFRQEIF 387
            IKFR  HRVF ++ F
Sbjct: 665  IKFRQAHRVFGRDTF 679



 Score =  125 bits (314), Expect = 9e-26
 Identities = 59/79 (74%), Positives = 66/79 (83%)
 Frame = -2

Query: 388 SXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVPQGVV 209
           S F+AFT HD  EGDIYLAFNAHD FI V +P+PP +R+WFRVVDTNL SPNDIVP GV 
Sbjct: 697 SKFLAFTFHDNSEGDIYLAFNAHDCFITVPMPAPPVKRKWFRVVDTNLVSPNDIVPGGVP 756

Query: 208 GIGDTYNVAPYSSILLQAK 152
           GI  TYN+AP+SSILLQAK
Sbjct: 757 GIAGTYNMAPFSSILLQAK 775


>ref|XP_021808237.1| isoamylase 3, chloroplastic isoform X4 [Prunus avium]
          Length = 774

 Score =  991 bits (2561), Expect = 0.0
 Identities = 470/618 (76%), Positives = 525/618 (84%), Gaps = 5/618 (0%)
 Frame = -1

Query: 2225 ECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
            +C+ H+ A  + QR            MSE  PS K+ PG   PLGVSE ++G NFA+FSQ
Sbjct: 61   DCVNHRTANAYGQRAQERVLEDEAPQMSETSPSWKTFPGQAFPLGVSEVDSGTNFAIFSQ 120

Query: 2045 HATSVTLCLSLP---GRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQ 1875
            HAT+VTLCLSLP   GR + GM+E  LD   NKTGDIWHIC++DLPRSNV+YGY +DGP+
Sbjct: 121  HATAVTLCLSLPERLGRVDGGMMEFSLDRNVNKTGDIWHICIKDLPRSNVLYGYCMDGPR 180

Query: 1874 GSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNI 1695
            G H+GHRFD+ VVLIDPYAKL++GRR FGDS+++FSK  GTYDF S PF WG+N+  PNI
Sbjct: 181  GWHEGHRFDSRVVLIDPYAKLVDGRRFFGDSSKKFSKFLGTYDFDSLPFAWGDNYKLPNI 240

Query: 1694 PEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEF 1515
            PEKDLV+YEMNVRAFTA +SSGL+ DIRGSYLG+I+KIPHL ELGINAVELLPVFEFDEF
Sbjct: 241  PEKDLVIYEMNVRAFTASESSGLDPDIRGSYLGVIEKIPHLQELGINAVELLPVFEFDEF 300

Query: 1514 EFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVIL 1335
            EFQRRPNPRDHMINTWGYSTINFFAPM+RYAS GGGP+ ASQEFK+MVKALH AGIEVIL
Sbjct: 301  EFQRRPNPRDHMINTWGYSTINFFAPMSRYASDGGGPLKASQEFKQMVKALHGAGIEVIL 360

Query: 1334 DVVYNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSGCGNTLNCNHPVVMELI 1161
            DVVYNHTNEADD NPYTTSFRGIDNK YYM+D   NGQLLNFSGCGNTLNCNHPVVMELI
Sbjct: 361  DVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLNNNGQLLNFSGCGNTLNCNHPVVMELI 420

Query: 1160 LDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDC 981
            +DSLRHWVTEYHVDGFRFDLASVLCRG DGSPL APP+++AIAKDSILSR KII+EPWDC
Sbjct: 421  MDSLRHWVTEYHVDGFRFDLASVLCRGADGSPLSAPPVIRAIAKDSILSRCKIISEPWDC 480

Query: 980  GGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYH 801
            GGLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV+GS+DL+ VNKRKPYH
Sbjct: 481  GGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRVSGSADLFKVNKRKPYH 540

Query: 800  GVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFR 621
             VNFVIAHDGFTLYDLVSYN+KH              NLSWNCG+EGET D  +KA+R R
Sbjct: 541  SVNFVIAHDGFTLYDLVSYNFKHNDANGEGGNDGSNDNLSWNCGYEGETDDSTVKAIRSR 600

Query: 620  QMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFF 441
            QMKNFHLALM+SQG PMMLMGDEYGH+RNGNNNSYGHDTAINHFQWG L+AR ++ FRFF
Sbjct: 601  QMKNFHLALMISQGTPMMLMGDEYGHSRNGNNNSYGHDTAINHFQWGLLEARNSNHFRFF 660

Query: 440  AEVIKFRLKHRVFRQEIF 387
             EVIKFR   RVF ++ F
Sbjct: 661  KEVIKFRRTRRVFGRDTF 678



 Score =  125 bits (313), Expect = 1e-25
 Identities = 58/79 (73%), Positives = 66/79 (83%)
 Frame = -2

Query: 388 SXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVPQGVV 209
           S F+AFT HD + GD+Y+AFNAHDYFIKV IP+ P +RRWFRVVDTNLESPND V  GV 
Sbjct: 696 SKFLAFTFHDNNGGDVYMAFNAHDYFIKVPIPAAPVKRRWFRVVDTNLESPNDFVLDGVP 755

Query: 208 GIGDTYNVAPYSSILLQAK 152
           GI  TYN+APYSSILL+AK
Sbjct: 756 GIKSTYNMAPYSSILLEAK 774


>ref|XP_021808236.1| isoamylase 3, chloroplastic isoform X3 [Prunus avium]
          Length = 781

 Score =  991 bits (2561), Expect = 0.0
 Identities = 470/618 (76%), Positives = 525/618 (84%), Gaps = 5/618 (0%)
 Frame = -1

Query: 2225 ECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
            +C+ H+ A  + QR            MSE  PS K+ PG   PLGVSE ++G NFA+FSQ
Sbjct: 61   DCVNHRTANAYGQRAQERVLEDEAPQMSETSPSWKTFPGQAFPLGVSEVDSGTNFAIFSQ 120

Query: 2045 HATSVTLCLSLP---GRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQ 1875
            HAT+VTLCLSLP   GR + GM+E  LD   NKTGDIWHIC++DLPRSNV+YGY +DGP+
Sbjct: 121  HATAVTLCLSLPERLGRVDGGMMEFSLDRNVNKTGDIWHICIKDLPRSNVLYGYCMDGPR 180

Query: 1874 GSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNI 1695
            G H+GHRFD+ VVLIDPYAKL++GRR FGDS+++FSK  GTYDF S PF WG+N+  PNI
Sbjct: 181  GWHEGHRFDSRVVLIDPYAKLVDGRRFFGDSSKKFSKFLGTYDFDSLPFAWGDNYKLPNI 240

Query: 1694 PEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEF 1515
            PEKDLV+YEMNVRAFTA +SSGL+ DIRGSYLG+I+KIPHL ELGINAVELLPVFEFDEF
Sbjct: 241  PEKDLVIYEMNVRAFTASESSGLDPDIRGSYLGVIEKIPHLQELGINAVELLPVFEFDEF 300

Query: 1514 EFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVIL 1335
            EFQRRPNPRDHMINTWGYSTINFFAPM+RYAS GGGP+ ASQEFK+MVKALH AGIEVIL
Sbjct: 301  EFQRRPNPRDHMINTWGYSTINFFAPMSRYASDGGGPLKASQEFKQMVKALHGAGIEVIL 360

Query: 1334 DVVYNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSGCGNTLNCNHPVVMELI 1161
            DVVYNHTNEADD NPYTTSFRGIDNK YYM+D   NGQLLNFSGCGNTLNCNHPVVMELI
Sbjct: 361  DVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLNNNGQLLNFSGCGNTLNCNHPVVMELI 420

Query: 1160 LDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDC 981
            +DSLRHWVTEYHVDGFRFDLASVLCRG DGSPL APP+++AIAKDSILSR KII+EPWDC
Sbjct: 421  MDSLRHWVTEYHVDGFRFDLASVLCRGADGSPLSAPPVIRAIAKDSILSRCKIISEPWDC 480

Query: 980  GGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYH 801
            GGLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV+GS+DL+ VNKRKPYH
Sbjct: 481  GGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRVSGSADLFKVNKRKPYH 540

Query: 800  GVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFR 621
             VNFVIAHDGFTLYDLVSYN+KH              NLSWNCG+EGET D  +KA+R R
Sbjct: 541  SVNFVIAHDGFTLYDLVSYNFKHNDANGEGGNDGSNDNLSWNCGYEGETDDSTVKAIRSR 600

Query: 620  QMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFF 441
            QMKNFHLALM+SQG PMMLMGDEYGH+RNGNNNSYGHDTAINHFQWG L+AR ++ FRFF
Sbjct: 601  QMKNFHLALMISQGTPMMLMGDEYGHSRNGNNNSYGHDTAINHFQWGLLEARNSNHFRFF 660

Query: 440  AEVIKFRLKHRVFRQEIF 387
             EVIKFR   RVF ++ F
Sbjct: 661  KEVIKFRRTRRVFGRDTF 678



 Score =  125 bits (313), Expect = 1e-25
 Identities = 58/79 (73%), Positives = 66/79 (83%)
 Frame = -2

Query: 388 SXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVPQGVV 209
           S F+AFT HD + GD+Y+AFNAHDYFIKV IP+ P +RRWFRVVDTNLESPND V  GV 
Sbjct: 703 SKFLAFTFHDNNGGDVYMAFNAHDYFIKVPIPAAPVKRRWFRVVDTNLESPNDFVLDGVP 762

Query: 208 GIGDTYNVAPYSSILLQAK 152
           GI  TYN+APYSSILL+AK
Sbjct: 763 GIKSTYNMAPYSSILLEAK 781


>ref|NP_001275220.1| isoamylase isoform 3 [Solanum tuberosum]
 gb|AAN15319.1| isoamylase isoform 3 [Solanum tuberosum]
          Length = 766

 Score =  986 bits (2550), Expect = 0.0
 Identities = 462/589 (78%), Positives = 514/589 (87%), Gaps = 2/589 (0%)
 Frame = -1

Query: 2147 MSEAGPSLKSIPGLPNPLGVSETENGINFALFSQHATSVTLCLSLPGRENDGMVELKLDP 1968
            M +  PS K  PGL +PLGVSETE+GINFA+FSQHA++VTLC+ LP   +DGM+EL LDP
Sbjct: 81   MLDTFPSFKVSPGLAHPLGVSETESGINFAIFSQHASAVTLCIILPKSVHDGMIELALDP 140

Query: 1967 QSNKTGDIWHICVQDLPRSNVIYGYRIDGPQGSHQGHRFDNSVVLIDPYAKLIEGRRVFG 1788
            Q N+TGDIWHIC+++LP+  V+YGYRIDGP+  H+GHRFD+S++L+DPYAKLIEGRRVFG
Sbjct: 141  QKNRTGDIWHICIKELPQGGVLYGYRIDGPRNWHEGHRFDDSIILVDPYAKLIEGRRVFG 200

Query: 1787 DSTERFSKLHGTYDFSSSPFDWGENHAQPNIPEKDLVVYEMNVRAFTADKSSGLEEDIRG 1608
            D + +  +  GTYDF+S PFDWGEN+  PNIPEKDLV+YEMNVRAFTAD++S L++D RG
Sbjct: 201  DESNKMCRFFGTYDFNSLPFDWGENYKLPNIPEKDLVIYEMNVRAFTADETSSLDQDQRG 260

Query: 1607 SYLGIIDKIPHLLELGINAVELLPVFEFDEFEFQRRPNPRDHMINTWGYSTINFFAPMTR 1428
            SYLG+I+KIPHLLELG+NAVELLPVFEFDE E QRRPNPRDHMINTWGYSTINFFAPM+R
Sbjct: 261  SYLGLIEKIPHLLELGVNAVELLPVFEFDELELQRRPNPRDHMINTWGYSTINFFAPMSR 320

Query: 1427 YASAGGGPICASQEFKEMVKALHLAGIEVILDVVYNHTNEADDNNPYTTSFRGIDNKTYY 1248
            YAS GGGP+ AS EFKEMVKALH AGIEVILDVVYNHTNEADD NPYTTSFRGIDNK YY
Sbjct: 321  YASCGGGPVRASWEFKEMVKALHGAGIEVILDVVYNHTNEADDENPYTTSFRGIDNKVYY 380

Query: 1247 MVD--GNGQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRGTD 1074
            MVD   N QLLNF+GCGNT NCNHP VMELIL+SLRHWVTEYHVDGFRFDLASVLCRGTD
Sbjct: 381  MVDLNNNAQLLNFAGCGNTFNCNHPTVMELILESLRHWVTEYHVDGFRFDLASVLCRGTD 440

Query: 1073 GSPLDAPPLVKAIAKDSILSRSKIIAEPWDCGGLYLVGKFPNWDRWAEWNGIYRDDLRRF 894
            G+P++APPLVKAI+KDS+LSR KIIAEPWDCGGLYLVGKFPNWDRWAEWNG YRDD+RRF
Sbjct: 441  GTPINAPPLVKAISKDSVLSRCKIIAEPWDCGGLYLVGKFPNWDRWAEWNGKYRDDIRRF 500

Query: 893  IKGDTGMKGGFATRVAGSSDLYNVNKRKPYHGVNFVIAHDGFTLYDLVSYNYKHXXXXXX 714
            IKGD GMKG FATR+AGS+DLY VNKRKPYH VNFVIAHDGFTLYDLVSYN KH      
Sbjct: 501  IKGDAGMKGNFATRIAGSADLYRVNKRKPYHSVNFVIAHDGFTLYDLVSYNNKHNDANGE 560

Query: 713  XXXXXXXXNLSWNCGHEGETSDVNIKALRFRQMKNFHLALMVSQGVPMMLMGDEYGHTRN 534
                    N SWNCG EGETSD NI ALR RQMKNFHLALMVSQG PMMLMGDEYGHTR 
Sbjct: 561  GGNDGCNDNFSWNCGIEGETSDANINALRSRQMKNFHLALMVSQGTPMMLMGDEYGHTRY 620

Query: 533  GNNNSYGHDTAINHFQWGQLDARKNDCFRFFAEVIKFRLKHRVFRQEIF 387
            GNNNSYGHDTAIN+FQWGQL+ARKND FRFF+++IKFRL H V R+E F
Sbjct: 621  GNNNSYGHDTAINNFQWGQLEARKNDHFRFFSKMIKFRLSHNVLRKENF 669



 Score =  117 bits (294), Expect = 2e-23
 Identities = 54/79 (68%), Positives = 66/79 (83%)
 Frame = -2

Query: 388 SXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVPQGVV 209
           S F+AF LHDG+ GDIYLAFNAH + IK +IPSPP+ R W+RVVDTNL+SP+D V +GV 
Sbjct: 687 SRFLAFMLHDGNGGDIYLAFNAHHFSIKTAIPSPPRNRSWYRVVDTNLKSPDDFVTEGVS 746

Query: 208 GIGDTYNVAPYSSILLQAK 152
           GI  TY+VAPYS+ILL+AK
Sbjct: 747 GISKTYDVAPYSAILLEAK 765


>ref|XP_010664352.1| PREDICTED: isoamylase 3, chloroplastic isoform X3 [Vitis vinifera]
          Length = 714

 Score =  985 bits (2547), Expect = 0.0
 Identities = 475/620 (76%), Positives = 518/620 (83%), Gaps = 7/620 (1%)
 Frame = -1

Query: 2225 ECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
            +C++ +    + +             ++E  PSLK +PG   PLGVSE ENGINFA+FSQ
Sbjct: 71   DCLRPRTTNAYGRHAQDRVLEEEAPQIAETSPSLKVLPGQAFPLGVSEVENGINFAIFSQ 130

Query: 2045 HATSVTLCLSLP-----GRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDG 1881
            HAT++ LCL LP      R +D MVEL LD   N+TGDIWHICV+DLPRSNV+YGY IDG
Sbjct: 131  HATAIVLCLFLPQRGKKDRMDDLMVELTLDADVNRTGDIWHICVEDLPRSNVLYGYCIDG 190

Query: 1880 PQGSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQP 1701
            P+  HQGHRFDNS VLIDPYAKL+EGRR FGD++ ++SK  GTYDF S PFDWG+N+  P
Sbjct: 191  PRDWHQGHRFDNSTVLIDPYAKLVEGRRFFGDASNKWSKFLGTYDFDSLPFDWGDNYKVP 250

Query: 1700 NIPEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFD 1521
            +IPEKDLV+YEMNVRAFTADKSSGL+ ++RGSYLG+I+KIPHLL+LGINAVELLPVFEFD
Sbjct: 251  SIPEKDLVIYEMNVRAFTADKSSGLDPNVRGSYLGVIEKIPHLLDLGINAVELLPVFEFD 310

Query: 1520 EFEFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEV 1341
            EFEFQRRPNPRDHMINTWGYSTINFFAPM+RYASAGGGPI AS+EFKEMVKALH AGIEV
Sbjct: 311  EFEFQRRPNPRDHMINTWGYSTINFFAPMSRYASAGGGPIKASREFKEMVKALHGAGIEV 370

Query: 1340 ILDVVYNHTNEADDNNPYTTSFRGIDNKTYYMVDGN--GQLLNFSGCGNTLNCNHPVVME 1167
            ILDVVYNHTNEADD NPYTTSFRGIDNK YYMVD N  GQLLNFSGCGNTLNCNHP+VME
Sbjct: 371  ILDVVYNHTNEADDENPYTTSFRGIDNKVYYMVDLNNEGQLLNFSGCGNTLNCNHPMVME 430

Query: 1166 LILDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPW 987
            LILDSLRHWV EYHVDGFRFDLASVLCRGTDGSPL+APP+++ IAKD ILSR KIIAEPW
Sbjct: 431  LILDSLRHWVIEYHVDGFRFDLASVLCRGTDGSPLNAPPIIREIAKDDILSRCKIIAEPW 490

Query: 986  DCGGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKP 807
            DCGGLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD G KG FATRVAGSSDLY VNKRKP
Sbjct: 491  DCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGTKGNFATRVAGSSDLYKVNKRKP 550

Query: 806  YHGVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALR 627
            YH VNFVIAHDGFTLYDLVSYN KH              N SWNCG EGET+D  IKALR
Sbjct: 551  YHSVNFVIAHDGFTLYDLVSYNSKHNNANGEGGKDGTNDNFSWNCGFEGETADAKIKALR 610

Query: 626  FRQMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFR 447
             RQMKNFHLALM SQG PMMLMGDEYGHTR GNNNSYGHDTAINHF WGQLD+RK D FR
Sbjct: 611  SRQMKNFHLALMCSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFLWGQLDSRKRDRFR 670

Query: 446  FFAEVIKFRLKHRVFRQEIF 387
            FF EVIKFR  H VF +E F
Sbjct: 671  FFKEVIKFRRMHHVFGRENF 690


>ref|XP_010664351.1| PREDICTED: isoamylase 3, chloroplastic isoform X1 [Vitis vinifera]
          Length = 787

 Score =  985 bits (2547), Expect = 0.0
 Identities = 475/620 (76%), Positives = 518/620 (83%), Gaps = 7/620 (1%)
 Frame = -1

Query: 2225 ECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
            +C++ +    + +             ++E  PSLK +PG   PLGVSE ENGINFA+FSQ
Sbjct: 71   DCLRPRTTNAYGRHAQDRVLEEEAPQIAETSPSLKVLPGQAFPLGVSEVENGINFAIFSQ 130

Query: 2045 HATSVTLCLSLP-----GRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDG 1881
            HAT++ LCL LP      R +D MVEL LD   N+TGDIWHICV+DLPRSNV+YGY IDG
Sbjct: 131  HATAIVLCLFLPQRGKKDRMDDLMVELTLDADVNRTGDIWHICVEDLPRSNVLYGYCIDG 190

Query: 1880 PQGSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQP 1701
            P+  HQGHRFDNS VLIDPYAKL+EGRR FGD++ ++SK  GTYDF S PFDWG+N+  P
Sbjct: 191  PRDWHQGHRFDNSTVLIDPYAKLVEGRRFFGDASNKWSKFLGTYDFDSLPFDWGDNYKVP 250

Query: 1700 NIPEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFD 1521
            +IPEKDLV+YEMNVRAFTADKSSGL+ ++RGSYLG+I+KIPHLL+LGINAVELLPVFEFD
Sbjct: 251  SIPEKDLVIYEMNVRAFTADKSSGLDPNVRGSYLGVIEKIPHLLDLGINAVELLPVFEFD 310

Query: 1520 EFEFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEV 1341
            EFEFQRRPNPRDHMINTWGYSTINFFAPM+RYASAGGGPI AS+EFKEMVKALH AGIEV
Sbjct: 311  EFEFQRRPNPRDHMINTWGYSTINFFAPMSRYASAGGGPIKASREFKEMVKALHGAGIEV 370

Query: 1340 ILDVVYNHTNEADDNNPYTTSFRGIDNKTYYMVDGN--GQLLNFSGCGNTLNCNHPVVME 1167
            ILDVVYNHTNEADD NPYTTSFRGIDNK YYMVD N  GQLLNFSGCGNTLNCNHP+VME
Sbjct: 371  ILDVVYNHTNEADDENPYTTSFRGIDNKVYYMVDLNNEGQLLNFSGCGNTLNCNHPMVME 430

Query: 1166 LILDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPW 987
            LILDSLRHWV EYHVDGFRFDLASVLCRGTDGSPL+APP+++ IAKD ILSR KIIAEPW
Sbjct: 431  LILDSLRHWVIEYHVDGFRFDLASVLCRGTDGSPLNAPPIIREIAKDDILSRCKIIAEPW 490

Query: 986  DCGGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKP 807
            DCGGLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD G KG FATRVAGSSDLY VNKRKP
Sbjct: 491  DCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGTKGNFATRVAGSSDLYKVNKRKP 550

Query: 806  YHGVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALR 627
            YH VNFVIAHDGFTLYDLVSYN KH              N SWNCG EGET+D  IKALR
Sbjct: 551  YHSVNFVIAHDGFTLYDLVSYNSKHNNANGEGGKDGTNDNFSWNCGFEGETADAKIKALR 610

Query: 626  FRQMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFR 447
             RQMKNFHLALM SQG PMMLMGDEYGHTR GNNNSYGHDTAINHF WGQLD+RK D FR
Sbjct: 611  SRQMKNFHLALMCSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFLWGQLDSRKRDRFR 670

Query: 446  FFAEVIKFRLKHRVFRQEIF 387
            FF EVIKFR  H VF +E F
Sbjct: 671  FFKEVIKFRRMHHVFGRENF 690



 Score =  128 bits (321), Expect = 1e-26
 Identities = 60/83 (72%), Positives = 67/83 (80%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S F+AFTLH  + GDIYLAFN HD+F+KV+IPSPP  RRWFRVVDTNLESP D V 
Sbjct: 704 DNYESKFLAFTLHANNGGDIYLAFNTHDFFVKVAIPSPPANRRWFRVVDTNLESPKDFVS 763

Query: 220 QGVVGIGDTYNVAPYSSILLQAK 152
           +GV GIG  YNVAPYSSILL+AK
Sbjct: 764 EGVPGIGSAYNVAPYSSILLEAK 786


>emb|CBI19205.3| unnamed protein product, partial [Vitis vinifera]
          Length = 775

 Score =  985 bits (2547), Expect = 0.0
 Identities = 475/620 (76%), Positives = 518/620 (83%), Gaps = 7/620 (1%)
 Frame = -1

Query: 2225 ECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
            +C++ +    + +             ++E  PSLK +PG   PLGVSE ENGINFA+FSQ
Sbjct: 59   DCLRPRTTNAYGRHAQDRVLEEEAPQIAETSPSLKVLPGQAFPLGVSEVENGINFAIFSQ 118

Query: 2045 HATSVTLCLSLP-----GRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDG 1881
            HAT++ LCL LP      R +D MVEL LD   N+TGDIWHICV+DLPRSNV+YGY IDG
Sbjct: 119  HATAIVLCLFLPQRGKKDRMDDLMVELTLDADVNRTGDIWHICVEDLPRSNVLYGYCIDG 178

Query: 1880 PQGSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQP 1701
            P+  HQGHRFDNS VLIDPYAKL+EGRR FGD++ ++SK  GTYDF S PFDWG+N+  P
Sbjct: 179  PRDWHQGHRFDNSTVLIDPYAKLVEGRRFFGDASNKWSKFLGTYDFDSLPFDWGDNYKVP 238

Query: 1700 NIPEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFD 1521
            +IPEKDLV+YEMNVRAFTADKSSGL+ ++RGSYLG+I+KIPHLL+LGINAVELLPVFEFD
Sbjct: 239  SIPEKDLVIYEMNVRAFTADKSSGLDPNVRGSYLGVIEKIPHLLDLGINAVELLPVFEFD 298

Query: 1520 EFEFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEV 1341
            EFEFQRRPNPRDHMINTWGYSTINFFAPM+RYASAGGGPI AS+EFKEMVKALH AGIEV
Sbjct: 299  EFEFQRRPNPRDHMINTWGYSTINFFAPMSRYASAGGGPIKASREFKEMVKALHGAGIEV 358

Query: 1340 ILDVVYNHTNEADDNNPYTTSFRGIDNKTYYMVDGN--GQLLNFSGCGNTLNCNHPVVME 1167
            ILDVVYNHTNEADD NPYTTSFRGIDNK YYMVD N  GQLLNFSGCGNTLNCNHP+VME
Sbjct: 359  ILDVVYNHTNEADDENPYTTSFRGIDNKVYYMVDLNNEGQLLNFSGCGNTLNCNHPMVME 418

Query: 1166 LILDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPW 987
            LILDSLRHWV EYHVDGFRFDLASVLCRGTDGSPL+APP+++ IAKD ILSR KIIAEPW
Sbjct: 419  LILDSLRHWVIEYHVDGFRFDLASVLCRGTDGSPLNAPPIIREIAKDDILSRCKIIAEPW 478

Query: 986  DCGGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKP 807
            DCGGLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD G KG FATRVAGSSDLY VNKRKP
Sbjct: 479  DCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGTKGNFATRVAGSSDLYKVNKRKP 538

Query: 806  YHGVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALR 627
            YH VNFVIAHDGFTLYDLVSYN KH              N SWNCG EGET+D  IKALR
Sbjct: 539  YHSVNFVIAHDGFTLYDLVSYNSKHNNANGEGGKDGTNDNFSWNCGFEGETADAKIKALR 598

Query: 626  FRQMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFR 447
             RQMKNFHLALM SQG PMMLMGDEYGHTR GNNNSYGHDTAINHF WGQLD+RK D FR
Sbjct: 599  SRQMKNFHLALMCSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFLWGQLDSRKRDRFR 658

Query: 446  FFAEVIKFRLKHRVFRQEIF 387
            FF EVIKFR  H VF +E F
Sbjct: 659  FFKEVIKFRRMHHVFGRENF 678



 Score =  128 bits (321), Expect = 1e-26
 Identities = 60/83 (72%), Positives = 67/83 (80%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S F+AFTLH  + GDIYLAFN HD+F+KV+IPSPP  RRWFRVVDTNLESP D V 
Sbjct: 692 DNYESKFLAFTLHANNGGDIYLAFNTHDFFVKVAIPSPPANRRWFRVVDTNLESPKDFVS 751

Query: 220 QGVVGIGDTYNVAPYSSILLQAK 152
           +GV GIG  YNVAPYSSILL+AK
Sbjct: 752 EGVPGIGSAYNVAPYSSILLEAK 774


>ref|XP_021808234.1| isoamylase 3, chloroplastic isoform X1 [Prunus avium]
          Length = 797

 Score =  984 bits (2545), Expect = 0.0
 Identities = 470/634 (74%), Positives = 525/634 (82%), Gaps = 21/634 (3%)
 Frame = -1

Query: 2225 ECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
            +C+ H+ A  + QR            MSE  PS K+ PG   PLGVSE ++G NFA+FSQ
Sbjct: 61   DCVNHRTANAYGQRAQERVLEDEAPQMSETSPSWKTFPGQAFPLGVSEVDSGTNFAIFSQ 120

Query: 2045 HATSVTLCLSLP-------------------GRENDGMVELKLDPQSNKTGDIWHICVQD 1923
            HAT+VTLCLSLP                   GR + GM+E  LD   NKTGDIWHIC++D
Sbjct: 121  HATAVTLCLSLPESFPQTHALMNIIFVKERLGRVDGGMMEFSLDRNVNKTGDIWHICIKD 180

Query: 1922 LPRSNVIYGYRIDGPQGSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDF 1743
            LPRSNV+YGY +DGP+G H+GHRFD+ VVLIDPYAKL++GRR FGDS+++FSK  GTYDF
Sbjct: 181  LPRSNVLYGYCMDGPRGWHEGHRFDSRVVLIDPYAKLVDGRRFFGDSSKKFSKFLGTYDF 240

Query: 1742 SSSPFDWGENHAQPNIPEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLEL 1563
             S PF WG+N+  PNIPEKDLV+YEMNVRAFTA +SSGL+ DIRGSYLG+I+KIPHL EL
Sbjct: 241  DSLPFAWGDNYKLPNIPEKDLVIYEMNVRAFTASESSGLDPDIRGSYLGVIEKIPHLQEL 300

Query: 1562 GINAVELLPVFEFDEFEFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEF 1383
            GINAVELLPVFEFDEFEFQRRPNPRDHMINTWGYSTINFFAPM+RYAS GGGP+ ASQEF
Sbjct: 301  GINAVELLPVFEFDEFEFQRRPNPRDHMINTWGYSTINFFAPMSRYASDGGGPLKASQEF 360

Query: 1382 KEMVKALHLAGIEVILDVVYNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSG 1209
            K+MVKALH AGIEVILDVVYNHTNEADD NPYTTSFRGIDNK YYM+D   NGQLLNFSG
Sbjct: 361  KQMVKALHGAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLNNNGQLLNFSG 420

Query: 1208 CGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAK 1029
            CGNTLNCNHPVVMELI+DSLRHWVTEYHVDGFRFDLASVLCRG DGSPL APP+++AIAK
Sbjct: 421  CGNTLNCNHPVVMELIMDSLRHWVTEYHVDGFRFDLASVLCRGADGSPLSAPPVIRAIAK 480

Query: 1028 DSILSRSKIIAEPWDCGGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRV 849
            DSILSR KII+EPWDCGGLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV
Sbjct: 481  DSILSRCKIISEPWDCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRV 540

Query: 848  AGSSDLYNVNKRKPYHGVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCG 669
            +GS+DL+ VNKRKPYH VNFVIAHDGFTLYDLVSYN+KH              NLSWNCG
Sbjct: 541  SGSADLFKVNKRKPYHSVNFVIAHDGFTLYDLVSYNFKHNDANGEGGNDGSNDNLSWNCG 600

Query: 668  HEGETSDVNIKALRFRQMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHF 489
            +EGET D  +KA+R RQMKNFHLALM+SQG PMMLMGDEYGH+RNGNNNSYGHDTAINHF
Sbjct: 601  YEGETDDSTVKAIRSRQMKNFHLALMISQGTPMMLMGDEYGHSRNGNNNSYGHDTAINHF 660

Query: 488  QWGQLDARKNDCFRFFAEVIKFRLKHRVFRQEIF 387
            QWG L+AR ++ FRFF EVIKFR   RVF ++ F
Sbjct: 661  QWGLLEARNSNHFRFFKEVIKFRRTRRVFGRDTF 694



 Score =  125 bits (313), Expect = 1e-25
 Identities = 58/79 (73%), Positives = 66/79 (83%)
 Frame = -2

Query: 388 SXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVPQGVV 209
           S F+AFT HD + GD+Y+AFNAHDYFIKV IP+ P +RRWFRVVDTNLESPND V  GV 
Sbjct: 719 SKFLAFTFHDNNGGDVYMAFNAHDYFIKVPIPAAPVKRRWFRVVDTNLESPNDFVLDGVP 778

Query: 208 GIGDTYNVAPYSSILLQAK 152
           GI  TYN+APYSSILL+AK
Sbjct: 779 GIKSTYNMAPYSSILLEAK 797


>ref|XP_021808235.1| isoamylase 3, chloroplastic isoform X2 [Prunus avium]
          Length = 790

 Score =  984 bits (2545), Expect = 0.0
 Identities = 470/634 (74%), Positives = 525/634 (82%), Gaps = 21/634 (3%)
 Frame = -1

Query: 2225 ECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQ 2046
            +C+ H+ A  + QR            MSE  PS K+ PG   PLGVSE ++G NFA+FSQ
Sbjct: 61   DCVNHRTANAYGQRAQERVLEDEAPQMSETSPSWKTFPGQAFPLGVSEVDSGTNFAIFSQ 120

Query: 2045 HATSVTLCLSLP-------------------GRENDGMVELKLDPQSNKTGDIWHICVQD 1923
            HAT+VTLCLSLP                   GR + GM+E  LD   NKTGDIWHIC++D
Sbjct: 121  HATAVTLCLSLPESFPQTHALMNIIFVKERLGRVDGGMMEFSLDRNVNKTGDIWHICIKD 180

Query: 1922 LPRSNVIYGYRIDGPQGSHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDF 1743
            LPRSNV+YGY +DGP+G H+GHRFD+ VVLIDPYAKL++GRR FGDS+++FSK  GTYDF
Sbjct: 181  LPRSNVLYGYCMDGPRGWHEGHRFDSRVVLIDPYAKLVDGRRFFGDSSKKFSKFLGTYDF 240

Query: 1742 SSSPFDWGENHAQPNIPEKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLEL 1563
             S PF WG+N+  PNIPEKDLV+YEMNVRAFTA +SSGL+ DIRGSYLG+I+KIPHL EL
Sbjct: 241  DSLPFAWGDNYKLPNIPEKDLVIYEMNVRAFTASESSGLDPDIRGSYLGVIEKIPHLQEL 300

Query: 1562 GINAVELLPVFEFDEFEFQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEF 1383
            GINAVELLPVFEFDEFEFQRRPNPRDHMINTWGYSTINFFAPM+RYAS GGGP+ ASQEF
Sbjct: 301  GINAVELLPVFEFDEFEFQRRPNPRDHMINTWGYSTINFFAPMSRYASDGGGPLKASQEF 360

Query: 1382 KEMVKALHLAGIEVILDVVYNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSG 1209
            K+MVKALH AGIEVILDVVYNHTNEADD NPYTTSFRGIDNK YYM+D   NGQLLNFSG
Sbjct: 361  KQMVKALHGAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLNNNGQLLNFSG 420

Query: 1208 CGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAK 1029
            CGNTLNCNHPVVMELI+DSLRHWVTEYHVDGFRFDLASVLCRG DGSPL APP+++AIAK
Sbjct: 421  CGNTLNCNHPVVMELIMDSLRHWVTEYHVDGFRFDLASVLCRGADGSPLSAPPVIRAIAK 480

Query: 1028 DSILSRSKIIAEPWDCGGLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRV 849
            DSILSR KII+EPWDCGGLYLVG+FPNWDRWAEWNG YRDDLRRFIKGD+GMKG FATRV
Sbjct: 481  DSILSRCKIISEPWDCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDSGMKGSFATRV 540

Query: 848  AGSSDLYNVNKRKPYHGVNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCG 669
            +GS+DL+ VNKRKPYH VNFVIAHDGFTLYDLVSYN+KH              NLSWNCG
Sbjct: 541  SGSADLFKVNKRKPYHSVNFVIAHDGFTLYDLVSYNFKHNDANGEGGNDGSNDNLSWNCG 600

Query: 668  HEGETSDVNIKALRFRQMKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHF 489
            +EGET D  +KA+R RQMKNFHLALM+SQG PMMLMGDEYGH+RNGNNNSYGHDTAINHF
Sbjct: 601  YEGETDDSTVKAIRSRQMKNFHLALMISQGTPMMLMGDEYGHSRNGNNNSYGHDTAINHF 660

Query: 488  QWGQLDARKNDCFRFFAEVIKFRLKHRVFRQEIF 387
            QWG L+AR ++ FRFF EVIKFR   RVF ++ F
Sbjct: 661  QWGLLEARNSNHFRFFKEVIKFRRTRRVFGRDTF 694



 Score =  125 bits (313), Expect = 1e-25
 Identities = 58/79 (73%), Positives = 66/79 (83%)
 Frame = -2

Query: 388 SXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVPQGVV 209
           S F+AFT HD + GD+Y+AFNAHDYFIKV IP+ P +RRWFRVVDTNLESPND V  GV 
Sbjct: 712 SKFLAFTFHDNNGGDVYMAFNAHDYFIKVPIPAAPVKRRWFRVVDTNLESPNDFVLDGVP 771

Query: 208 GIGDTYNVAPYSSILLQAK 152
           GI  TYN+APYSSILL+AK
Sbjct: 772 GIKSTYNMAPYSSILLEAK 790


>ref|XP_023872402.1| isoamylase 3, chloroplastic [Quercus suber]
          Length = 775

 Score =  984 bits (2543), Expect = 0.0
 Identities = 481/677 (71%), Positives = 540/677 (79%), Gaps = 3/677 (0%)
 Frame = -1

Query: 2408 NPTKLFKSVCLPDASLFRMIL-NTTTNSPIPLSAFRANRVILSRNAXXXXXXXXXXXKVI 2232
            NPTK         ASL   ++ N T+ S I  S     R+ LS+ A              
Sbjct: 14   NPTK---------ASLIASVMSNNTSRSCIGPSHVSEMRLKLSKRASGGNAPGLSSKT-- 62

Query: 2231 GSECMKHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALF 2052
                +KH    V+ QR            +SE  PS+K  PG   PLGVSE E+GINFA+F
Sbjct: 63   -GGHVKHWTTNVYGQRAQERVLEEEAPQVSETTPSIKIFPGQAFPLGVSEVESGINFAIF 121

Query: 2051 SQHATSVTLCLSLPGRENDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQG 1872
            SQHA++VTLCLS P R +  M+EL LDP  NKTGDIWHIC++DLPRSNV+YGYRIDGP+ 
Sbjct: 122  SQHASAVTLCLSFPERWDGKMMELTLDPHVNKTGDIWHICIEDLPRSNVLYGYRIDGPRD 181

Query: 1871 SHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNIP 1692
             H+GHRFD+S+VL+DPYAKL+EGRR FGD++++FS   GTYDF S PFDWGE +  PNIP
Sbjct: 182  WHRGHRFDSSIVLLDPYAKLVEGRRYFGDASKKFSNFLGTYDFESLPFDWGETYKLPNIP 241

Query: 1691 EKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEFE 1512
            EKDLV+YEMNVRAFT D+SS L+  IRGSYLG+I+KIPHLLELGINAVELLPVFEFDEFE
Sbjct: 242  EKDLVIYEMNVRAFTFDESSELDPKIRGSYLGVIEKIPHLLELGINAVELLPVFEFDEFE 301

Query: 1511 FQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVILD 1332
            FQRRPNPRDH+INTWGYSTINFFAPM+RYAS GGGP+ AS+E KEMVKALH AGIEVILD
Sbjct: 302  FQRRPNPRDHLINTWGYSTINFFAPMSRYASDGGGPLNASRELKEMVKALHGAGIEVILD 361

Query: 1331 VVYNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSGCGNTLNCNHPVVMELIL 1158
            VV+NHTNEADD NPYTTSFRGIDNK YYM+D   NGQLLNFSGCGNTLNCNHPVVMELIL
Sbjct: 362  VVFNHTNEADDANPYTTSFRGIDNKVYYMLDVNNNGQLLNFSGCGNTLNCNHPVVMELIL 421

Query: 1157 DSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDCG 978
            DSLRHWV +YHVDGFRFDLASVLCRGTDGSPL+APP+++AIAKDS+LSR KIIAEPWDCG
Sbjct: 422  DSLRHWVVDYHVDGFRFDLASVLCRGTDGSPLNAPPIIRAIAKDSVLSRCKIIAEPWDCG 481

Query: 977  GLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYHG 798
            GLYLVG FPNWDRWAEWNG YRDD+RR+IKGDTGMKG FATRV+GS+DLY VNKRKPYH 
Sbjct: 482  GLYLVGGFPNWDRWAEWNGKYRDDIRRYIKGDTGMKGSFATRVSGSADLYKVNKRKPYHS 541

Query: 797  VNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFRQ 618
            VNFVIAHDGFTLYDLVSYN+KH              N SWNCG EGET D++IK+LR RQ
Sbjct: 542  VNFVIAHDGFTLYDLVSYNFKHNDANGEGGNDGGNDNFSWNCGFEGETDDISIKSLRSRQ 601

Query: 617  MKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFFA 438
            MKNFHLALM+SQG PMMLMGDEYGHTR GNNNSYGHDT INHFQWGQLD RK+  FRFFA
Sbjct: 602  MKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTTINHFQWGQLDTRKSSLFRFFA 661

Query: 437  EVIKFRLKHRVFRQEIF 387
            + I+FR  H VF +E F
Sbjct: 662  KAIQFRQTHHVFHRENF 678



 Score =  124 bits (310), Expect = 3e-25
 Identities = 58/83 (69%), Positives = 66/83 (79%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S F+AFTLHD + GDIYLAFNAHDY ++ SIP PP  + WFRVVDTNLESP+D V 
Sbjct: 692 DNYESRFLAFTLHDNNGGDIYLAFNAHDYSVRASIPRPPVNKSWFRVVDTNLESPDDFVT 751

Query: 220 QGVVGIGDTYNVAPYSSILLQAK 152
            GV GIG TYNVAP+SSILL+AK
Sbjct: 752 DGVPGIGSTYNVAPFSSILLEAK 774


>gb|AOQ26243.1| ISA3 [Actinidia deliciosa]
          Length = 790

 Score =  982 bits (2538), Expect = 0.0
 Identities = 461/617 (74%), Positives = 522/617 (84%), Gaps = 7/617 (1%)
 Frame = -1

Query: 2216 KHKPAYVHSQRXXXXXXXXXXXVMSEAGPSLKSIPGLPNPLGVSETENGINFALFSQHAT 2037
            +HK    + +R              E+ PS +  PG   PLGVSE E+GINF++FSQHAT
Sbjct: 77   RHKSTNAYGRRAKERVIEEEVAQTLESRPSFRVFPGQAYPLGVSELESGINFSIFSQHAT 136

Query: 2036 SVTLCLSLPGRE-----NDGMVELKLDPQSNKTGDIWHICVQDLPRSNVIYGYRIDGPQG 1872
             VTLCLSLP R      + GMVEL LDP  NKTGDIWHICV+DLPRS+++YGYR+DGP+G
Sbjct: 137  KVTLCLSLPERGKHDKLDGGMVELTLDPHVNKTGDIWHICVEDLPRSDILYGYRVDGPRG 196

Query: 1871 SHQGHRFDNSVVLIDPYAKLIEGRRVFGDSTERFSKLHGTYDFSSSPFDWGENHAQPNIP 1692
             HQGHRFD+S+ L+DPYAKL+EGRR+FGD++ + +K  GTYDF+S PF+WG+N+  PNIP
Sbjct: 197  WHQGHRFDSSIFLVDPYAKLVEGRRLFGDTSHKMAKFLGTYDFNSLPFNWGDNYKLPNIP 256

Query: 1691 EKDLVVYEMNVRAFTADKSSGLEEDIRGSYLGIIDKIPHLLELGINAVELLPVFEFDEFE 1512
            EKDLV+YEM++RAFTAD+SSGL+   RGSYLG+I+KIPHL+ELGINAVELLP+FEFDE E
Sbjct: 257  EKDLVIYEMSIRAFTADESSGLDPSTRGSYLGVIEKIPHLVELGINAVELLPIFEFDELE 316

Query: 1511 FQRRPNPRDHMINTWGYSTINFFAPMTRYASAGGGPICASQEFKEMVKALHLAGIEVILD 1332
            FQRR NPRDH+INTWGYSTINFFAPM+RYASAGGGP  AS+EFKEMVKALH AGIEVILD
Sbjct: 317  FQRRRNPRDHLINTWGYSTINFFAPMSRYASAGGGPTNASREFKEMVKALHDAGIEVILD 376

Query: 1331 VVYNHTNEADDNNPYTTSFRGIDNKTYYMVD--GNGQLLNFSGCGNTLNCNHPVVMELIL 1158
            VVYNHTNEADD+NPYT SFRGIDNK YYMVD   NGQLLN++GCGNTLNCNHPVVMELIL
Sbjct: 377  VVYNHTNEADDDNPYTISFRGIDNKVYYMVDLNNNGQLLNYTGCGNTLNCNHPVVMELIL 436

Query: 1157 DSLRHWVTEYHVDGFRFDLASVLCRGTDGSPLDAPPLVKAIAKDSILSRSKIIAEPWDCG 978
            DSLRHWV +YHVDGFRFDLASVLCRGTDGSPLDAPPL++AIAKD +LSR KIIAEPWDCG
Sbjct: 437  DSLRHWVVDYHVDGFRFDLASVLCRGTDGSPLDAPPLIRAIAKDPLLSRCKIIAEPWDCG 496

Query: 977  GLYLVGKFPNWDRWAEWNGIYRDDLRRFIKGDTGMKGGFATRVAGSSDLYNVNKRKPYHG 798
            GLYLVGKFPNWDRWAEWNG YRDD+R+FIKGD G+KG FATRVAGS+DLY VNKRKPYH 
Sbjct: 497  GLYLVGKFPNWDRWAEWNGKYRDDMRKFIKGDAGVKGSFATRVAGSADLYKVNKRKPYHS 556

Query: 797  VNFVIAHDGFTLYDLVSYNYKHXXXXXXXXXXXXXXNLSWNCGHEGETSDVNIKALRFRQ 618
            +NFVIAHDGFTLYDLVSYN+KH              N SWNCG EGET+D NIKALRFRQ
Sbjct: 557  INFVIAHDGFTLYDLVSYNFKHNDANGESGNDGSNDNFSWNCGCEGETTDTNIKALRFRQ 616

Query: 617  MKNFHLALMVSQGVPMMLMGDEYGHTRNGNNNSYGHDTAINHFQWGQLDARKNDCFRFFA 438
            MKNFHLALM+SQG PMM+MGDEYGHTR GNNNSYGHDTAINHFQWGQL+ARKND FRFF+
Sbjct: 617  MKNFHLALMISQGTPMMMMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKNDHFRFFS 676

Query: 437  EVIKFRLKHRVFRQEIF 387
            E+IKFR    +F +E F
Sbjct: 677  EIIKFRRSRHIFGRESF 693



 Score =  140 bits (354), Expect = 1e-30
 Identities = 65/84 (77%), Positives = 74/84 (88%)
 Frame = -2

Query: 400 DKKFSXFIAFTLHDGDEGDIYLAFNAHDYFIKVSIPSPPQQRRWFRVVDTNLESPNDIVP 221
           D   S F+AFT H+ + GDIYLAFNAHDYF+KV+IPSPPQ+RRWFRVVDTNL+SP DIVP
Sbjct: 707 DNTESKFLAFTFHEKNGGDIYLAFNAHDYFVKVAIPSPPQKRRWFRVVDTNLKSPGDIVP 766

Query: 220 QGVVGIGDTYNVAPYSSILLQAKP 149
           +GV GIG TYNVAPYSSILL+AKP
Sbjct: 767 EGVPGIGKTYNVAPYSSILLEAKP 790