BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00048227
         (1814 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KVH88407.1| hypothetical protein Ccrd_023896 [Cynara carduncu...   894   0.0  
ref|XP_023744695.1| 4-coumarate--CoA ligase 2 [Lactuca sativa] >...   884   0.0  
gb|AQY55797.1| 4-coumarate:coenzyme A ligase 2 [Ixeris dentata v...   868   0.0  
ref|XP_022006757.1| 4-coumarate--CoA ligase 2 isoform X2 [Helian...   849   0.0  
ref|XP_022006756.1| 4-coumarate--CoA ligase 2 isoform X1 [Helian...   844   0.0  
ref|XP_022882151.1| 4-coumarate--CoA ligase 2-like [Olea europae...   781   0.0  
ref|XP_024179550.1| 4-coumarate--CoA ligase 2 [Rosa chinensis] >...   781   0.0  
gb|ASU87410.1| 4-coumarate-CoA ligase [Camellia sinensis]             780   0.0  
ref|XP_021816145.1| 4-coumarate--CoA ligase 2-like [Prunus avium...   780   0.0  
ref|XP_009351826.1| PREDICTED: 4-coumarate--CoA ligase 2-like [P...   779   0.0  
ref|XP_008234565.1| PREDICTED: 4-coumarate--CoA ligase 2 [Prunus...   778   0.0  
gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshu...   776   0.0  
ref|XP_018817126.1| PREDICTED: 4-coumarate--CoA ligase 2-like [J...   776   0.0  
ref|XP_022896767.1| 4-coumarate--CoA ligase 2-like [Olea europae...   775   0.0  
ref|XP_021282810.1| 4-coumarate--CoA ligase 2 [Herrania umbratica]    775   0.0  
dbj|GAV76541.1| AMP-binding domain-containing protein/DUF4009 do...   777   0.0  
ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate-...   775   0.0  
gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]   774   0.0  
ref|XP_008376904.1| PREDICTED: 4-coumarate--CoA ligase 2 [Malus ...   775   0.0  
gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]            775   0.0  

>gb|KVH88407.1| hypothetical protein Ccrd_023896 [Cynara cardunculus var. scolymus]
          Length = 583

 Score =  894 bits (2311), Expect = 0.0
 Identities = 461/567 (81%), Positives = 494/567 (87%), Gaps = 4/567 (0%)
 Frame = +3

Query: 3    PPPLVXXXXXXXXXXXXXXXXYNVEKDDSSSKNVVFRSKLPDLSYIPNHVPLHTYCFENV 182
            PPPLV                +N   DD SS++VVFRSKLPDL  IPNH+PLHTYCFEN+
Sbjct: 14   PPPLVTSSSSASTCNNNINHTHNHNVDDDSSRHVVFRSKLPDLPNIPNHIPLHTYCFENL 73

Query: 183  SRFSNRPCLVMXXXXXXXXXXQTHLLSRKIAAGLSTLGVSKGDVIMLLLQNCAEFALTFM 362
            SRFS+RPCLV+          QTH LSRKIAAGLSTLG++KGDVIMLLLQNCAEFALTFM
Sbjct: 74   SRFSDRPCLVVGSTGTTYSFSQTHFLSRKIAAGLSTLGITKGDVIMLLLQNCAEFALTFM 133

Query: 363  AASMIGAVITTANPFYTSGEIFKQFKSSNSKIIITQSQYVNKLVIRESNGEEEYIGQ--- 533
            AASMIGA+ T ANP YT+GEIFKQF SSNSK+IITQ+QYV+KL  R+       +GQ   
Sbjct: 134  AASMIGAITTAANPLYTAGEIFKQFNSSNSKMIITQAQYVDKL--RDPGEHNPKMGQDFP 191

Query: 534  VITIDDPPKKCLHFSFLLQNNEENIPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLIT 713
            VIT+D+PP+KCLHFS L + +E NIP V IDP+DAVALPFSSGTTGLPKGVILTHKSLIT
Sbjct: 192  VITVDEPPEKCLHFSVLWKTDENNIPSVTIDPDDAVALPFSSGTTGLPKGVILTHKSLIT 251

Query: 714  SVAQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLE 893
            SVAQQVDGENPNLYLK+DDVVLCVLPLFHIYSLNSVLLCSLR+GAAVVLMPKFEIRSLL+
Sbjct: 252  SVAQQVDGENPNLYLKQDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVVLMPKFEIRSLLD 311

Query: 894  LIQKYRVTXXXXXXXXXXXXXKNPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAI 1073
            LIQ+YRVT             KNPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAI
Sbjct: 312  LIQRYRVTVAAVVPPLVLALAKNPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAI 371

Query: 1074 FGQGYGMTEAGPVLSMSAAFAKNPLATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEI 1253
            FGQGYGMTEAGPVLSMSAAFAK PLATKSGSCG+VVRNAELKVID DTH SLGYNQPGEI
Sbjct: 372  FGQGYGMTEAGPVLSMSAAFAKQPLATKSGSCGSVVRNAELKVIDLDTHYSLGYNQPGEI 431

Query: 1254 CIRGPQIMKGYLNDAESTAATIDEEGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVP 1433
            CIRGPQIMKGYLNDA+STAATIDE+GWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVP
Sbjct: 432  CIRGPQIMKGYLNDAKSTAATIDEQGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVP 491

Query: 1434 PAELEALLVSHPCIADAAVVPQKDDAAGEVPVAFVVLLSGM-ALSEEAIKDHIAKQVVFY 1610
            PAELEALLVSHPCIADAAVVPQKDDAAGEVPVAFVVLL+GM  L+EEA+KD+IAKQVVFY
Sbjct: 492  PAELEALLVSHPCIADAAVVPQKDDAAGEVPVAFVVLLNGMQELTEEAVKDYIAKQVVFY 551

Query: 1611 KKLHKVYFVTSIPKSPSGKILRKDLRA 1691
            KKLHKVYFVTSIPKSPSGKILRKDLRA
Sbjct: 552  KKLHKVYFVTSIPKSPSGKILRKDLRA 578


>ref|XP_023744695.1| 4-coumarate--CoA ligase 2 [Lactuca sativa]
 gb|PLY65502.1| hypothetical protein LSAT_3X461 [Lactuca sativa]
          Length = 582

 Score =  884 bits (2285), Expect = 0.0
 Identities = 453/567 (79%), Positives = 490/567 (86%), Gaps = 4/567 (0%)
 Frame = +3

Query: 3    PPPLVXXXXXXXXXXXXXXXXYNVEK-DDSSSKNVVFRSKLPDLSYIPNHVPLHTYCFEN 179
            PPP +                 N+ K DD S+++V+FRSKLPDL  I NH+PLHTYCFEN
Sbjct: 13   PPPPLASSSSSPASTSNNTNTNNIHKVDDGSNRHVIFRSKLPDLPNIANHIPLHTYCFEN 72

Query: 180  VSRFSNRPCLVMXXXXXXXXXXQTHLLSRKIAAGLSTLGVSKGDVIMLLLQNCAEFALTF 359
            +SRFS++PCLV+          QTH+LSRKIA GLS LG+SKGDVIMLLLQNCAEFALTF
Sbjct: 73   LSRFSDKPCLVVGSTGATYTFSQTHILSRKIAVGLSALGISKGDVIMLLLQNCAEFALTF 132

Query: 360  MAASMIGAVITTANPFYTSGEIFKQFKSSNSKIIITQSQYVNKLVIRESNGEEEYIGQ-- 533
            MAASMIGAV T ANPFYT+GEIFKQF SSNSK+IITQ+QYV+KL  R+       +GQ  
Sbjct: 133  MAASMIGAVTTAANPFYTAGEIFKQFNSSNSKMIITQAQYVDKL--RDHGEHNPKMGQDF 190

Query: 534  -VITIDDPPKKCLHFSFLLQNNEENIPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLI 710
             VIT+DDPP+KCLHFS L + NE NIP V IDP+DAVALPFSSGTTGLPKGVILTHKSLI
Sbjct: 191  PVITVDDPPEKCLHFSMLYRTNENNIPSVTIDPDDAVALPFSSGTTGLPKGVILTHKSLI 250

Query: 711  TSVAQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLL 890
            TSVAQQVDGENPNLYLK++DVVLCVLPLFHIYSLNSVLLCSLR+GA+VVLMPKFEIRSLL
Sbjct: 251  TSVAQQVDGENPNLYLKQEDVVLCVLPLFHIYSLNSVLLCSLRAGASVVLMPKFEIRSLL 310

Query: 891  ELIQKYRVTXXXXXXXXXXXXXKNPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQA 1070
            ELIQ+YRVT             KNPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQA
Sbjct: 311  ELIQRYRVTVAAVVPPLVLALAKNPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQA 370

Query: 1071 IFGQGYGMTEAGPVLSMSAAFAKNPLATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGE 1250
            IFGQGYGMTEAGPVLSMS AFAK PL TKSGSCG+VVRNAELKVIDPDT+SSLGYNQPGE
Sbjct: 371  IFGQGYGMTEAGPVLSMSVAFAKQPLPTKSGSCGSVVRNAELKVIDPDTYSSLGYNQPGE 430

Query: 1251 ICIRGPQIMKGYLNDAESTAATIDEEGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQV 1430
            ICIRGPQIMKGYLNDA+STAATIDEEGWLHTGDIGYVDHD+EVFIVDRVKELIKFKGFQV
Sbjct: 431  ICIRGPQIMKGYLNDAKSTAATIDEEGWLHTGDIGYVDHDEEVFIVDRVKELIKFKGFQV 490

Query: 1431 PPAELEALLVSHPCIADAAVVPQKDDAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFY 1610
            PPAELE LLVSHPCIADAAVV QKDDAAGEVPVAFVVLL+GM L++EA+KD+IAKQVVFY
Sbjct: 491  PPAELEGLLVSHPCIADAAVVSQKDDAAGEVPVAFVVLLNGMELTQEAVKDYIAKQVVFY 550

Query: 1611 KKLHKVYFVTSIPKSPSGKILRKDLRA 1691
            KKLHKVYFV SIPKSPSGKILRKDLRA
Sbjct: 551  KKLHKVYFVASIPKSPSGKILRKDLRA 577


>gb|AQY55797.1| 4-coumarate:coenzyme A ligase 2 [Ixeris dentata var. albiflora]
          Length = 582

 Score =  868 bits (2242), Expect = 0.0
 Identities = 442/543 (81%), Positives = 478/543 (88%), Gaps = 1/543 (0%)
 Frame = +3

Query: 66   YNVEKDDSSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXX 245
            +N   DDSS  +VVFRSKLPDL  IPNH+PLHTYCFEN+ RFS +PCLV+          
Sbjct: 37   HNNVDDDSSRHHVVFRSKLPDLPNIPNHIPLHTYCFENLPRFSEKPCLVVGPTGTTYSFS 96

Query: 246  QTHLLSRKIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEI 425
            QT++LSRKIAAGL TLG+SKGDVIMLLLQNCAEFALTFMAASMIGAV T ANPFYT+GEI
Sbjct: 97   QTYILSRKIAAGLYTLGISKGDVIMLLLQNCAEFALTFMAASMIGAVTTAANPFYTAGEI 156

Query: 426  FKQFKSSNSKIIITQSQYVNKLVIRESNGEEEYIGQVITIDDPPKKCLHFSFLLQNNEE- 602
            FKQFK SNSK+IITQSQYV KL  R+       +G VITID+PP++CLHFS L    +E 
Sbjct: 157  FKQFKCSNSKMIITQSQYVEKL--RDHGKHNPKMGPVITIDEPPEECLHFSVLSGTTDET 214

Query: 603  NIPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLC 782
            NIP V IDP+D VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLK++DVVLC
Sbjct: 215  NIPYVTIDPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKQEDVVLC 274

Query: 783  VLPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKN 962
            VLPLFHIYSLNSVLLCS+R+GA+VVLMPKFEIRSLLELIQ+YRVT             KN
Sbjct: 275  VLPLFHIYSLNSVLLCSMRAGASVVLMPKFEIRSLLELIQRYRVTVAAVVPPMVLSLAKN 334

Query: 963  PMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKN 1142
            PMVGSYDLSSIRVVLSGAAPLGKELEDGLR RVPQAIFGQGYGMTEAGPVLSMSAAFAK 
Sbjct: 335  PMVGSYDLSSIRVVLSGAAPLGKELEDGLRRRVPQAIFGQGYGMTEAGPVLSMSAAFAKQ 394

Query: 1143 PLATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATID 1322
            PL TKSGSCG+VVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDA+STAATID
Sbjct: 395  PLPTKSGSCGSVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAQSTAATID 454

Query: 1323 EEGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQK 1502
            E GWLHTGDIGYVD DDEVFIVDR KELIKFKGFQVPPAELE LLV+HPCIADAAVVPQK
Sbjct: 455  EHGWLHTGDIGYVDDDDEVFIVDRAKELIKFKGFQVPPAELEGLLVNHPCIADAAVVPQK 514

Query: 1503 DDAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKD 1682
            DDAAGEVP+AFVVLL+G  +++EA+KD+IAKQVVFYKKLHKVYFV+SIPKSPSGKILRKD
Sbjct: 515  DDAAGEVPIAFVVLLTGKEITQEAVKDYIAKQVVFYKKLHKVYFVSSIPKSPSGKILRKD 574

Query: 1683 LRA 1691
            L+A
Sbjct: 575  LKA 577


>ref|XP_022006757.1| 4-coumarate--CoA ligase 2 isoform X2 [Helianthus annuus]
 gb|OTG00025.1| putative 4-coumarate--CoA ligase 2 [Helianthus annuus]
          Length = 564

 Score =  849 bits (2193), Expect = 0.0
 Identities = 441/541 (81%), Positives = 473/541 (87%)
 Frame = +3

Query: 69   NVEKDDSSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQ 248
            N   DDSS++  VFRSKLPDL  IPNH+PLH+YCF+N+SRFS+RPCLV+          Q
Sbjct: 30   NNNHDDSSTR--VFRSKLPDLPCIPNHMPLHSYCFQNLSRFSDRPCLVVGSTGTTYSYSQ 87

Query: 249  THLLSRKIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIF 428
            THLLSR+IAAGLS+LGVSKGDVIMLLLQNCAEFAL F+AASMIGAV T ANPF T+GEI 
Sbjct: 88   THLLSRRIAAGLSSLGVSKGDVIMLLLQNCAEFALAFIAASMIGAVTTAANPFCTAGEIS 147

Query: 429  KQFKSSNSKIIITQSQYVNKLVIRESNGEEEYIGQVITIDDPPKKCLHFSFLLQNNEENI 608
            KQ  SS +KIIITQ+QYV KL          +   VIT+DDPP+KC+HFS LL   + +I
Sbjct: 148  KQLTSSKAKIIITQAQYVEKL--------RPHCVPVITVDDPPEKCMHFSVLLLC-QNSI 198

Query: 609  PPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVL 788
            P V IDP+DAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLK+DDVVLCVL
Sbjct: 199  PSVTIDPDDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKQDDVVLCVL 258

Query: 789  PLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNPM 968
            PLFHIYSLNSVLLCSLR+GAAVVLMPKFEIRSLLE IQ+YRVT             KNPM
Sbjct: 259  PLFHIYSLNSVLLCSLRAGAAVVLMPKFEIRSLLEHIQRYRVTVAAVVPPLVLALAKNPM 318

Query: 969  VGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNPL 1148
            VGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAK PL
Sbjct: 319  VGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKQPL 378

Query: 1149 ATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDEE 1328
             TKSGSCG+VVRNAELKVIDPDTH SLGYNQPGEICIRGPQIMKGYLNDA STAATIDE 
Sbjct: 379  PTKSGSCGSVVRNAELKVIDPDTHHSLGYNQPGEICIRGPQIMKGYLNDAMSTAATIDEL 438

Query: 1329 GWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDD 1508
            GWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKD 
Sbjct: 439  GWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDI 498

Query: 1509 AAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDLR 1688
            AAGEVPVAFVVL++GM ++EE +KD+IAKQVVFYKKLHKVYFV+SIPKSPSGKILRKDLR
Sbjct: 499  AAGEVPVAFVVLVTGMEITEEDVKDYIAKQVVFYKKLHKVYFVSSIPKSPSGKILRKDLR 558

Query: 1689 A 1691
            A
Sbjct: 559  A 559


>ref|XP_022006756.1| 4-coumarate--CoA ligase 2 isoform X1 [Helianthus annuus]
          Length = 566

 Score =  844 bits (2180), Expect = 0.0
 Identities = 441/543 (81%), Positives = 473/543 (87%), Gaps = 2/543 (0%)
 Frame = +3

Query: 69   NVEKDDSSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQ 248
            N   DDSS++  VFRSKLPDL  IPNH+PLH+YCF+N+SRFS+RPCLV+          Q
Sbjct: 30   NNNHDDSSTR--VFRSKLPDLPCIPNHMPLHSYCFQNLSRFSDRPCLVVGSTGTTYSYSQ 87

Query: 249  THLLSRKIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIF 428
            THLLSR+IAAGLS+LGVSKGDVIMLLLQNCAEFAL F+AASMIGAV T ANPF T+GEI 
Sbjct: 88   THLLSRRIAAGLSSLGVSKGDVIMLLLQNCAEFALAFIAASMIGAVTTAANPFCTAGEIS 147

Query: 429  KQFKSSNSKIIITQSQYVNKLVIRESNGEEEYIGQVITIDDPPKKCLHFSFLLQNNEENI 608
            KQ  SS +KIIITQ+QYV KL          +   VIT+DDPP+KC+HFS LL   + +I
Sbjct: 148  KQLTSSKAKIIITQAQYVEKL--------RPHCVPVITVDDPPEKCMHFSVLLLC-QNSI 198

Query: 609  PPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVL 788
            P V IDP+DAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLK+DDVVLCVL
Sbjct: 199  PSVTIDPDDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKQDDVVLCVL 258

Query: 789  PLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNPM 968
            PLFHIYSLNSVLLCSLR+GAAVVLMPKFEIRSLLE IQ+YRVT             KNPM
Sbjct: 259  PLFHIYSLNSVLLCSLRAGAAVVLMPKFEIRSLLEHIQRYRVTVAAVVPPLVLALAKNPM 318

Query: 969  VGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNPL 1148
            VGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAK PL
Sbjct: 319  VGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKQPL 378

Query: 1149 ATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDEE 1328
             TKSGSCG+VVRNAELKVIDPDTH SLGYNQPGEICIRGPQIMKGYLNDA STAATIDE 
Sbjct: 379  PTKSGSCGSVVRNAELKVIDPDTHHSLGYNQPGEICIRGPQIMKGYLNDAMSTAATIDEL 438

Query: 1329 GWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDD 1508
            GWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKD 
Sbjct: 439  GWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDI 498

Query: 1509 AAGEVPVAFVVLLSGMALSEEAIKDHIAK--QVVFYKKLHKVYFVTSIPKSPSGKILRKD 1682
            AAGEVPVAFVVL++GM ++EE +KD+IAK  QVVFYKKLHKVYFV+SIPKSPSGKILRKD
Sbjct: 499  AAGEVPVAFVVLVTGMEITEEDVKDYIAKQMQVVFYKKLHKVYFVSSIPKSPSGKILRKD 558

Query: 1683 LRA 1691
            LRA
Sbjct: 559  LRA 561


>ref|XP_022882151.1| 4-coumarate--CoA ligase 2-like [Olea europaea var. sylvestris]
          Length = 568

 Score =  781 bits (2016), Expect = 0.0
 Identities = 402/538 (74%), Positives = 449/538 (83%), Gaps = 3/538 (0%)
 Frame = +3

Query: 87   SSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSR 266
            SS +  VF SKLP +  I NH+PLH YCFE+++++ +RPCL+M          +THL+ R
Sbjct: 27   SSEETHVFISKLPQIP-ISNHLPLHAYCFEDLAQYPDRPCLIMGNGGKTYSFSETHLVCR 85

Query: 267  KIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSS 446
            K AAGLS LGV KGDVIM+LLQNCAEF  TFM ASMIGAV TTANPFYTS EIFKQF +S
Sbjct: 86   KTAAGLSILGVKKGDVIMVLLQNCAEFVFTFMGASMIGAVTTTANPFYTSSEIFKQFGAS 145

Query: 447  NSKIIITQSQYVNKLVIRESNGEEEYIGQ---VITIDDPPKKCLHFSFLLQNNEENIPPV 617
             +K+IITQSQYV KL  R+   +   +G+   VITIDDPP+ CLHFS L + NE +IP V
Sbjct: 146  KAKLIITQSQYVCKL--RDQGQDYPKLGEDFTVITIDDPPENCLHFSVLSEANENDIPSV 203

Query: 618  IIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLF 797
             +DPND VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLK DDVVLCVLPLF
Sbjct: 204  CVDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKSDDVVLCVLPLF 263

Query: 798  HIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNPMVGS 977
            HIYSLNSVLLCSLR+GA V+LM KFEI SLLELIQ++RV+             KNPMV  
Sbjct: 264  HIYSLNSVLLCSLRAGAGVLLMQKFEIGSLLELIQRHRVSVAAVVPPLVLALAKNPMVDK 323

Query: 978  YDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNPLATK 1157
            +DLSSIRVVLSGAAPLGK+LE  LR+RVPQAIFGQGYGMTEAGPVLSMS AFAK P+ TK
Sbjct: 324  FDLSSIRVVLSGAAPLGKDLETALRNRVPQAIFGQGYGMTEAGPVLSMSPAFAKQPMPTK 383

Query: 1158 SGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDEEGWL 1337
            SGSCGNVVRNAELKV+DP++ SSL  NQPGEICIRGPQIMKGYLND E+TA TID +GWL
Sbjct: 384  SGSCGNVVRNAELKVVDPESGSSLPRNQPGEICIRGPQIMKGYLNDVEATANTIDVDGWL 443

Query: 1338 HTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDDAAG 1517
            HTGD+GYVD DD+VFIVDRVKELIKFKGFQVPPAELEALLVSHP IADAAVVPQKD+AAG
Sbjct: 444  HTGDVGYVDDDDDVFIVDRVKELIKFKGFQVPPAELEALLVSHPNIADAAVVPQKDEAAG 503

Query: 1518 EVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDLRA 1691
            EVPVAFVV  +G  L+EEA+K+ IAKQVVFYKKLHKVYF+ SIPKSP+GKILRKDLRA
Sbjct: 504  EVPVAFVVRSNGFELTEEAVKEFIAKQVVFYKKLHKVYFIHSIPKSPAGKILRKDLRA 561


>ref|XP_024179550.1| 4-coumarate--CoA ligase 2 [Rosa chinensis]
 gb|PRQ60707.1| putative acid--thiol ligase [Rosa chinensis]
          Length = 588

 Score =  781 bits (2018), Expect = 0.0
 Identities = 398/533 (74%), Positives = 443/533 (83%), Gaps = 4/533 (0%)
 Frame = +3

Query: 105  VFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSRKIAAGL 284
            VF+SKLPDL  I NH+PLHTYCFEN+S FS+RPCL++          +THL+S+K  AGL
Sbjct: 49   VFKSKLPDLPNISNHLPLHTYCFENISDFSDRPCLIVGSTGKTYTFSETHLISQKTGAGL 108

Query: 285  STLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSSNSKIII 464
            S LG++KGDVIM+LL NCAEF   FM ASMIGAV TTANPFYT+ EIFKQFK+SN+K+II
Sbjct: 109  SKLGITKGDVIMILLPNCAEFTFAFMGASMIGAVTTTANPFYTASEIFKQFKASNAKLII 168

Query: 465  TQSQYVNKLVIRESNGEE-EYIG---QVITIDDPPKKCLHFSFLLQNNEENIPPVIIDPN 632
            TQS YV+KL    ++ ++   +G   QV+TIDDPP  CLHFS L   NE  +P V IDP 
Sbjct: 169  TQSTYVDKLRHSGTSSDQYPMLGEHFQVVTIDDPPMNCLHFSVLSDANENELPEVSIDPE 228

Query: 633  DAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHIYSL 812
            D VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHIYSL
Sbjct: 229  DPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHIYSL 288

Query: 813  NSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNPMVGSYDLSS 992
            NSVLLCSLR+GAAV++M KFEI +LLELIQKYRV+             KNPMV  YDLSS
Sbjct: 289  NSVLLCSLRAGAAVLVMHKFEIGTLLELIQKYRVSVAAVVPPLVIALAKNPMVADYDLSS 348

Query: 993  IRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNPLATKSGSCG 1172
            IR +LSGAAPLGK+LE+ LRSRVPQA+ GQGYGMTEAGPVLSM  AFAK P  TKSGSCG
Sbjct: 349  IRAILSGAAPLGKDLEEALRSRVPQAVLGQGYGMTEAGPVLSMCLAFAKQPFPTKSGSCG 408

Query: 1173 NVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDEEGWLHTGDI 1352
             VVRNAELKVI+P+T  SLGYNQPGEICIRG QIMKGYLND E+TA T+D EGWLHTGD+
Sbjct: 409  TVVRNAELKVIEPETGRSLGYNQPGEICIRGSQIMKGYLNDDEATATTVDVEGWLHTGDV 468

Query: 1353 GYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDDAAGEVPVA 1532
            GYVD D+EVFIVDRVKELIKFKGFQVPPAELEALLV H  IADAAVVPQKDDAAGEVPVA
Sbjct: 469  GYVDDDNEVFIVDRVKELIKFKGFQVPPAELEALLVCHQSIADAAVVPQKDDAAGEVPVA 528

Query: 1533 FVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDLRA 1691
            FVV  +G  L+EEAIK+ +AKQVVFYK+LHKVYFV +IPKSPSGKILRKDLRA
Sbjct: 529  FVVRSNGNELTEEAIKEFVAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRA 581


>gb|ASU87410.1| 4-coumarate-CoA ligase [Camellia sinensis]
          Length = 570

 Score =  780 bits (2013), Expect = 0.0
 Identities = 401/538 (74%), Positives = 450/538 (83%), Gaps = 3/538 (0%)
 Frame = +3

Query: 87   SSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSR 266
            SSS+  VFRSKLPD+  I NH+PLHTYCF+N+S+F +RPCL++          +THL+SR
Sbjct: 25   SSSETHVFRSKLPDIP-ISNHLPLHTYCFQNLSQFPDRPCLLIGSTGKSYSFSETHLVSR 83

Query: 267  KIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSS 446
            K+A+GLS LG+ KGDVIMLLLQNCAEF   FM ASMIGAV TTANPFYT  E+FKQF +S
Sbjct: 84   KVASGLSLLGIKKGDVIMLLLQNCAEFVFAFMGASMIGAVTTTANPFYTPAEVFKQFNAS 143

Query: 447  NSKIIITQSQYVNKLVIRESNGEEEYIGQ---VITIDDPPKKCLHFSFLLQNNEENIPPV 617
             SK+IITQSQYV+KL  R++      +G+   VITIDDPP+ CLHFS L + NE  IP V
Sbjct: 144  KSKLIITQSQYVDKL--RDAGDNFPKLGEEFSVITIDDPPENCLHFSVLSEANESEIPTV 201

Query: 618  IIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLF 797
             IDP+D VALPFSSGTTGLPKGVILTHKSLI+SV QQVDGENPNL+LK +D+VLCVLPLF
Sbjct: 202  SIDPDDPVALPFSSGTTGLPKGVILTHKSLISSVPQQVDGENPNLHLKGEDMVLCVLPLF 261

Query: 798  HIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNPMVGS 977
            HIYSLNSVLLCSLR+GA V+LM KFEI +LLELIQ+YRV+             KNPMV +
Sbjct: 262  HIYSLNSVLLCSLRAGAGVLLMHKFEIGALLELIQRYRVSVAAAVPPLVLALAKNPMVVT 321

Query: 978  YDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNPLATK 1157
            +DLSSIR+VLSGAAPLGKELE+ LR+RVPQAIFGQGYGMTEAGPVLSM  AFAK P  TK
Sbjct: 322  FDLSSIRMVLSGAAPLGKELEEALRTRVPQAIFGQGYGMTEAGPVLSMCLAFAKQPFPTK 381

Query: 1158 SGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDEEGWL 1337
            SGSCG VVRNAELKVIDP+T  SLG N  GEICIRGPQIMKGYLNDAE+TA TID +GWL
Sbjct: 382  SGSCGTVVRNAELKVIDPETGCSLGPNHSGEICIRGPQIMKGYLNDAEATATTIDVDGWL 441

Query: 1338 HTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDDAAG 1517
            HTGDIGYVD DDEVFIVDRVKELIKFKGFQVPPAELEALLVSHP IADAAVVPQKDD AG
Sbjct: 442  HTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDDVAG 501

Query: 1518 EVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDLRA 1691
            EVPVAFVV  +G+ L+E+A+K+ IAKQVVFYKKLHKVYFV +IPKSPSGKILRKDLRA
Sbjct: 502  EVPVAFVVRSNGLELTEDAVKEFIAKQVVFYKKLHKVYFVHAIPKSPSGKILRKDLRA 559


>ref|XP_021816145.1| 4-coumarate--CoA ligase 2-like [Prunus avium]
 gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
          Length = 604

 Score =  780 bits (2013), Expect = 0.0
 Identities = 392/544 (72%), Positives = 455/544 (83%), Gaps = 9/544 (1%)
 Frame = +3

Query: 87   SSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSR 266
            +++ N VF+SKLPD+  IPNH+PLHTYCF+ +S FS+RPCL++           T+L+S+
Sbjct: 53   TTTTNHVFKSKLPDIP-IPNHLPLHTYCFQKLSEFSDRPCLIVSSTGKSYSFSDTYLISQ 111

Query: 267  KIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSS 446
            K  AGLS LG+ KGDVIM+LLQNCAEF   FM ASMIGA+ TTANPFYT+ EIFKQFK+S
Sbjct: 112  KTGAGLSNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAITTTANPFYTATEIFKQFKNS 171

Query: 447  NSKIIITQSQYVNKLVIRESNG----EEEYIG-----QVITIDDPPKKCLHFSFLLQNNE 599
            N+K+IIT SQ+V+KL    ++G    +  Y+      +V+T+DDPP+ CLHFS + + NE
Sbjct: 172  NAKLIITHSQFVDKLREAPADGAAPDQNNYLKLGEHFKVVTVDDPPENCLHFSVISEANE 231

Query: 600  ENIPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVL 779
            + +P V+IDP D VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVL
Sbjct: 232  KELPEVVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVL 291

Query: 780  CVLPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXK 959
            CVLPLFHIYSLNSV+LCSLR+GAAV++M KFEI +LLELIQ+YRV+             K
Sbjct: 292  CVLPLFHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAK 351

Query: 960  NPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAK 1139
            NPMV  +DLSSIRVVLSGAAPLGKELE+ LRSRVP A+ GQGYGMTEAGPVLSM  AFAK
Sbjct: 352  NPMVAQFDLSSIRVVLSGAAPLGKELEEALRSRVPGAVLGQGYGMTEAGPVLSMCLAFAK 411

Query: 1140 NPLATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATI 1319
             PL +KSGSCG V+RNAELKVID +T  SLGYNQPGEICIRG QIMKGYLNDAE+TA T+
Sbjct: 412  EPLPSKSGSCGTVIRNAELKVIDSETGCSLGYNQPGEICIRGSQIMKGYLNDAEATATTV 471

Query: 1320 DEEGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQ 1499
            D+EGWLHTGD+GYVD D+EVFIVDRVKELIKFKGFQVPPAELE+LLVSHP IADAAVVPQ
Sbjct: 472  DKEGWLHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQ 531

Query: 1500 KDDAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRK 1679
            KD+AAGEVPVAFVV  +G+ L+EEA+K+ IAKQVVFYK+LHKVYFV +IPKSPSGKILRK
Sbjct: 532  KDEAAGEVPVAFVVRSNGLQLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRK 591

Query: 1680 DLRA 1691
            DLRA
Sbjct: 592  DLRA 595


>ref|XP_009351826.1| PREDICTED: 4-coumarate--CoA ligase 2-like [Pyrus x bretschneideri]
          Length = 606

 Score =  779 bits (2011), Expect = 0.0
 Identities = 391/546 (71%), Positives = 454/546 (83%), Gaps = 11/546 (2%)
 Frame = +3

Query: 87   SSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSR 266
            +++ N VFRSKLPD+  IPNH+PLH+YCF+N++ FS+RPCL++          +THL+S+
Sbjct: 52   TATTNHVFRSKLPDIP-IPNHLPLHSYCFQNLAEFSDRPCLIVGSTGKSYSFSETHLISQ 110

Query: 267  KIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSS 446
            K  AGLS LG+ KGDVIM+LLQNCAEF   FM ASMIGAV TTANPFYT+ EIFKQ K++
Sbjct: 111  KTGAGLSNLGIQKGDVIMILLQNCAEFVFAFMGASMIGAVTTTANPFYTAAEIFKQVKAA 170

Query: 447  NSKIIITQSQYVNKLVIRESNGE-----------EEYIGQVITIDDPPKKCLHFSFLLQN 593
            N+K+IITQSQY NKL    S+ +           E++  +V+TIDDPP+ CLHFS L + 
Sbjct: 171  NAKLIITQSQYANKLHEHPSSADGADQNNFPKLGEDF--KVVTIDDPPENCLHFSVLSEA 228

Query: 594  NEENIPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDV 773
            NE+ +P V+ID  D VALPFSSGTTGLPKGVILTHKSL+TSVAQQVDGENPNLYLKEDDV
Sbjct: 229  NEKELPDVVIDTEDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKEDDV 288

Query: 774  VLCVLPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXX 953
            VLCVLPLFHI+SLNSVLLCSLR+GA V+LM KFEI +LLELIQ+YRV+            
Sbjct: 289  VLCVLPLFHIFSLNSVLLCSLRAGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIAL 348

Query: 954  XKNPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAF 1133
             KNPMV  +DLSSIRVVLSGAAPLGKELE+ L+SRVP+A+ GQGYGMTEAGPVLSM  AF
Sbjct: 349  AKNPMVAEFDLSSIRVVLSGAAPLGKELEEALKSRVPEAVLGQGYGMTEAGPVLSMCMAF 408

Query: 1134 AKNPLATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAA 1313
            A+ P+ TKSGSCG VVRNAELKV+DP+T  SLGYNQPGEICIRG QIMKGYLNDA +TA 
Sbjct: 409  AREPMPTKSGSCGTVVRNAELKVLDPETGLSLGYNQPGEICIRGSQIMKGYLNDAAATAT 468

Query: 1314 TIDEEGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVV 1493
            T+D EGWLHTGD+GYVD DDEVFIVDRVKELIKFKGFQVPPAELE+LL+SHP IADAAVV
Sbjct: 469  TVDTEGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELESLLISHPSIADAAVV 528

Query: 1494 PQKDDAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKIL 1673
            PQKDDAAGEVPVAFVV  SG+ L+EE++K+ IAKQVVFYK+LHKV+FV +IPKSP+GKIL
Sbjct: 529  PQKDDAAGEVPVAFVVRSSGLELTEESVKEFIAKQVVFYKRLHKVHFVHAIPKSPAGKIL 588

Query: 1674 RKDLRA 1691
            RKDLRA
Sbjct: 589  RKDLRA 594


>ref|XP_008234565.1| PREDICTED: 4-coumarate--CoA ligase 2 [Prunus mume]
          Length = 606

 Score =  778 bits (2009), Expect = 0.0
 Identities = 393/544 (72%), Positives = 455/544 (83%), Gaps = 9/544 (1%)
 Frame = +3

Query: 87   SSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSR 266
            +++ N VF+SKLPD+  IPNH+PLHTYCF+ +S FS+RPCL++           T+L+S+
Sbjct: 55   TNTTNHVFKSKLPDIP-IPNHLPLHTYCFQKLSEFSDRPCLIVGSTGKSYSFSDTYLISQ 113

Query: 267  KIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSS 446
            K  AGLS LG+ KGDVIM+LLQNCAEF   FM ASMIGAV TTANPFYT+ EIFKQFK+S
Sbjct: 114  KTGAGLSNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAVTTTANPFYTAAEIFKQFKAS 173

Query: 447  NSKIIITQSQYVNKLVIRESNG----EEEY--IGQ---VITIDDPPKKCLHFSFLLQNNE 599
            N+K+IIT SQ+V+KL    ++G    +  Y  +G+   V+T+DDPP+ CLHFS + + NE
Sbjct: 174  NAKLIITHSQFVDKLREAPADGAAPDQNNYPKLGEHFKVVTVDDPPENCLHFSVISEANE 233

Query: 600  ENIPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVL 779
              +P V+IDP D VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVL
Sbjct: 234  TELPEVVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVL 293

Query: 780  CVLPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXK 959
            CVLPLFHIYSLNSV+LCSLR+GAAV++M KFEI +LLELIQ+YRV+             K
Sbjct: 294  CVLPLFHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAK 353

Query: 960  NPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAK 1139
            NPMV  +DLSSIRVVLSGAAPLGKELE+ L+ RVP+A+ GQGYGMTEAGPVLSM  AFAK
Sbjct: 354  NPMVAQFDLSSIRVVLSGAAPLGKELEEALKGRVPRAVLGQGYGMTEAGPVLSMCLAFAK 413

Query: 1140 NPLATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATI 1319
             PL +KSGSCG VVRNAELKVID +T  SLGYNQPGEICIRG QIMKGYLNDAE+TA T+
Sbjct: 414  EPLPSKSGSCGTVVRNAELKVIDAETGCSLGYNQPGEICIRGSQIMKGYLNDAEATATTV 473

Query: 1320 DEEGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQ 1499
            D+EGWLHTGD+GYVD D+EVFIVDRVKELIKFKGFQVPPAELE+LLVSHP IADAAVVPQ
Sbjct: 474  DKEGWLHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQ 533

Query: 1500 KDDAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRK 1679
            KD+AAGEVPVAFVV  +G+ L+EEA+K+ IAKQVVFYK+LHKVYFV +IPKSPSGKILRK
Sbjct: 534  KDEAAGEVPVAFVVRSNGLQLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRK 593

Query: 1680 DLRA 1691
            DLRA
Sbjct: 594  DLRA 597


>gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshurica]
          Length = 573

 Score =  776 bits (2004), Expect = 0.0
 Identities = 400/538 (74%), Positives = 446/538 (82%), Gaps = 3/538 (0%)
 Frame = +3

Query: 87   SSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSR 266
            SS +  VF SKLP +  I NH+PLHTYCF N++++  RPCL++          +THLL R
Sbjct: 27   SSEETHVFISKLPQIP-ISNHLPLHTYCFGNLAQYPERPCLIVGSTGKTYSFSETHLLCR 85

Query: 267  KIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSS 446
            K AAGLS LG+ KGDVIM+LLQNC EF  TFM ASMIGAV TTANPFYTS E+FKQF +S
Sbjct: 86   KTAAGLSKLGLKKGDVIMVLLQNCPEFVFTFMGASMIGAVTTTANPFYTSSEVFKQFGAS 145

Query: 447  NSKIIITQSQYVNKLVIRESNGEEEYIGQ---VITIDDPPKKCLHFSFLLQNNEENIPPV 617
             +K+IITQSQYV+KL  R+       +G+   VITIDDPP+ CLHFS L + NE +IP V
Sbjct: 146  EAKMIITQSQYVDKL--RDPGQNNPKLGEDFTVITIDDPPENCLHFSVLSEANENDIPSV 203

Query: 618  IIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLF 797
             IDPND VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLK DDVVLCVLPLF
Sbjct: 204  SIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKSDDVVLCVLPLF 263

Query: 798  HIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNPMVGS 977
            HIYSLNSVLLCSLR+GA V+LM KFEI S LELIQ++RV+             KNP+V  
Sbjct: 264  HIYSLNSVLLCSLRAGAGVLLMQKFEIGSFLELIQRHRVSVAAVVPPLVLALAKNPLVDK 323

Query: 978  YDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNPLATK 1157
            +DLSSIRVVLSGAAPLGKELE  LR+RVPQAIFGQGYGMTEAGPVLSMS AFAK P+ TK
Sbjct: 324  FDLSSIRVVLSGAAPLGKELEAALRNRVPQAIFGQGYGMTEAGPVLSMSTAFAKQPILTK 383

Query: 1158 SGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDEEGWL 1337
            SGSCGNVVRNAELKV+DP++ SSL  NQPGEICIRGPQIMKGYLND E+TA TID +GWL
Sbjct: 384  SGSCGNVVRNAELKVVDPESGSSLPRNQPGEICIRGPQIMKGYLNDDEATANTIDVDGWL 443

Query: 1338 HTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDDAAG 1517
            HTGDIGYVD DD++FIVDRVKELIKFKGFQVPPAELEALLVSHP IADAAVVPQKD+AAG
Sbjct: 444  HTGDIGYVDDDDDIFIVDRVKELIKFKGFQVPPAELEALLVSHPNIADAAVVPQKDEAAG 503

Query: 1518 EVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDLRA 1691
            EVPVAFVV  +G  LSEEA+K+ IAKQVVFYKKLHKVYF+ +IPKSP+GKILRKDLRA
Sbjct: 504  EVPVAFVVRSNGFELSEEAVKEFIAKQVVFYKKLHKVYFIHAIPKSPAGKILRKDLRA 561


>ref|XP_018817126.1| PREDICTED: 4-coumarate--CoA ligase 2-like [Juglans regia]
          Length = 572

 Score =  776 bits (2003), Expect = 0.0
 Identities = 392/542 (72%), Positives = 451/542 (83%), Gaps = 3/542 (0%)
 Frame = +3

Query: 75   EKDDSSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTH 254
            +  ++S  + VFRSKLPD+  I NH+PLHTYCFE++S FS+RPCL++          +TH
Sbjct: 25   QNHEASETDRVFRSKLPDIP-ISNHLPLHTYCFEHLSEFSDRPCLIVGSTGKSFTFAETH 83

Query: 255  LLSRKIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQ 434
            L+S+K+AAGL  LG+ KGD IM+LLQNCAEF  +F+ ASM+GAV TTANPFYTS E+FKQ
Sbjct: 84   LISQKVAAGLFNLGIRKGDAIMILLQNCAEFVFSFIGASMLGAVTTTANPFYTSAEVFKQ 143

Query: 435  FKSSNSKIIITQSQYVNKLVIRESNGEEEYIGQ---VITIDDPPKKCLHFSFLLQNNEEN 605
            F++S +K+II+QSQYV+KL  RE   +   +G+   VIT+DDPP+KCLHFS L + NE  
Sbjct: 144  FRASRAKLIISQSQYVDKL--REPGEDYPKLGEDFTVITVDDPPEKCLHFSVLSEANESE 201

Query: 606  IPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCV 785
            IP V IDP D VALPFSSGTTGLPKGV+LTHKSLITSVAQQVDGENPNLYLK +DVVLCV
Sbjct: 202  IPTVSIDPEDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLKPEDVVLCV 261

Query: 786  LPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNP 965
            LPLFHIYSLNSVLLCSLR GAAV+LM KFEI SLLELIQ++R++             KNP
Sbjct: 262  LPLFHIYSLNSVLLCSLRVGAAVLLMQKFEIGSLLELIQRHRISVVAVVPPLVLALAKNP 321

Query: 966  MVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNP 1145
            +V  +DLSSIRVVLSGAAPLGKELE+ L+SR+PQAIFGQGYGMTEAGPVLSM   FAK P
Sbjct: 322  IVDKFDLSSIRVVLSGAAPLGKELEEALQSRIPQAIFGQGYGMTEAGPVLSMCLGFAKQP 381

Query: 1146 LATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDE 1325
              TKSGSCG VVRNAELKVIDP+T SS G+NQPGEICIRG QIMKGYLND E+TA+TID+
Sbjct: 382  FPTKSGSCGTVVRNAELKVIDPETGSSFGFNQPGEICIRGSQIMKGYLNDPEATASTIDD 441

Query: 1326 EGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKD 1505
            EGWLHTGDIGYVD D E+FIVDRVKELIKFKGFQVPPAELEALLVSHP IADAAVVPQK 
Sbjct: 442  EGWLHTGDIGYVDDDYEIFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKK 501

Query: 1506 DAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDL 1685
            D AGEVPVAFVV  +G  L+EEA+K+ +AK+VVFYK+LHKVYFV +IPKSPSGKILRKDL
Sbjct: 502  DVAGEVPVAFVVRSNGFELTEEAVKEFVAKEVVFYKRLHKVYFVHAIPKSPSGKILRKDL 561

Query: 1686 RA 1691
            RA
Sbjct: 562  RA 563


>ref|XP_022896767.1| 4-coumarate--CoA ligase 2-like [Olea europaea var. sylvestris]
          Length = 570

 Score =  775 bits (2001), Expect = 0.0
 Identities = 397/538 (73%), Positives = 446/538 (82%), Gaps = 3/538 (0%)
 Frame = +3

Query: 87   SSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSR 266
            SS +  VF SKLP +  I NH+PLHTYCFEN++++  RPCL++          +THLL +
Sbjct: 27   SSDETHVFISKLPQIP-ISNHLPLHTYCFENLAQYPERPCLIVGSTGKTYSFSETHLLCQ 85

Query: 267  KIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSS 446
            K AAGLS LGV KGDVIM+LLQNC EF  TF+ ASMIGA+ TTANPFYTS E+FKQ  +S
Sbjct: 86   KTAAGLSNLGVKKGDVIMVLLQNCPEFVFTFIGASMIGAITTTANPFYTSSEVFKQLGAS 145

Query: 447  NSKIIITQSQYVNKLVIRESNGEEEYIGQ---VITIDDPPKKCLHFSFLLQNNEENIPPV 617
             +K+IITQSQYV+KL  R+       +G+   VITIDDPP+ CLHFS L + NE +IP +
Sbjct: 146  KAKLIITQSQYVDKL--RDPGQNNPKVGEDFTVITIDDPPENCLHFSVLSEANENDIPSI 203

Query: 618  IIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLF 797
             IDPND VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLK DDVVLCVLPLF
Sbjct: 204  SIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKSDDVVLCVLPLF 263

Query: 798  HIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNPMVGS 977
            HIYSLNSVLLCSLR+GA V+LM KFEI S LELIQ++RV+             KNPMV  
Sbjct: 264  HIYSLNSVLLCSLRAGAGVLLMQKFEIGSFLELIQRHRVSVAAVVPPLVLALAKNPMVDK 323

Query: 978  YDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNPLATK 1157
            +DLSSIRVVLSGAAPLGKELE  LR+RVPQAIFGQGYGMTEAGPVLSMS AFAK P+ TK
Sbjct: 324  FDLSSIRVVLSGAAPLGKELEAALRNRVPQAIFGQGYGMTEAGPVLSMSPAFAKQPILTK 383

Query: 1158 SGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDEEGWL 1337
            SGSCGNVVRNAELKV+DP++ +SL  NQPGEICIRGPQIMKGYLNDAE+TA TID +GWL
Sbjct: 384  SGSCGNVVRNAELKVVDPESGTSLPRNQPGEICIRGPQIMKGYLNDAEATANTIDVDGWL 443

Query: 1338 HTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDDAAG 1517
            HTGDIGYVD DD VFIVDRVKELIKFKGFQVPPAELEALL+SHP IADAAVVPQKD+AAG
Sbjct: 444  HTGDIGYVDDDDNVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEAAG 503

Query: 1518 EVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDLRA 1691
            EVPVAFVV  +G  L+EEA+K+ IAKQVVFYKKLHKVYF+ +IPKSP+GKILRKDLRA
Sbjct: 504  EVPVAFVVRSNGFELTEEAVKEFIAKQVVFYKKLHKVYFIHAIPKSPAGKILRKDLRA 561


>ref|XP_021282810.1| 4-coumarate--CoA ligase 2 [Herrania umbratica]
          Length = 574

 Score =  775 bits (2001), Expect = 0.0
 Identities = 395/532 (74%), Positives = 440/532 (82%), Gaps = 3/532 (0%)
 Frame = +3

Query: 105  VFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSRKIAAGL 284
            VFRSKLPD+  I NH+PLHTYCFEN+S F ++PCL+           +THL++RK AAGL
Sbjct: 35   VFRSKLPDIP-ISNHLPLHTYCFENLSSFPDKPCLISGSSGKTYSFSETHLIARKTAAGL 93

Query: 285  STLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSSNSKIII 464
            S LG+ KGDVIM+LLQNCAEF  +FM ASMIGAV TTANPFYT  EIFKQ+K++ +K+I+
Sbjct: 94   SNLGIRKGDVIMILLQNCAEFVFSFMGASMIGAVSTTANPFYTPTEIFKQYKAAQAKLIV 153

Query: 465  TQSQYVNKLVIRESNGEEEYIGQ---VITIDDPPKKCLHFSFLLQNNEENIPPVIIDPND 635
            TQSQYV+KL    +N     IG+   VITIDDPP+ CLHF+ L + NE  IP V IDP+D
Sbjct: 154  TQSQYVDKLKDTSNNENFPKIGEDFKVITIDDPPENCLHFTVLSEANENEIPQVSIDPDD 213

Query: 636  AVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHIYSLN 815
             VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLK DDVVLCVLPLFHIYSLN
Sbjct: 214  PVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKHDDVVLCVLPLFHIYSLN 273

Query: 816  SVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNPMVGSYDLSSI 995
            SVLLCSLR+GAAV+LM KFEI +LLELIQ++RV+             KNPMV  YDLSSI
Sbjct: 274  SVLLCSLRAGAAVLLMQKFEIGALLELIQRHRVSVAAVVPPLVLALAKNPMVAQYDLSSI 333

Query: 996  RVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNPLATKSGSCGN 1175
            RVVLSGAAPLGKELE+ LR RVPQA+ GQGYGMTEAGPVLSM   FAK P  TKSGSCG 
Sbjct: 334  RVVLSGAAPLGKELEEALRGRVPQAVLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGT 393

Query: 1176 VVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDEEGWLHTGDIG 1355
            VVRNAELKVIDP+T  SL YNQPGEICIRG QIMKGYLND  STA TID EGWLHTGDIG
Sbjct: 394  VVRNAELKVIDPETGFSLDYNQPGEICIRGSQIMKGYLNDTSSTATTIDVEGWLHTGDIG 453

Query: 1356 YVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDDAAGEVPVAF 1535
            YVD DDE+FIVDRVKE+IKFKGFQVPPAELE+LLVSHP IADAAVVPQKD+ AGEVPVAF
Sbjct: 454  YVDDDDEIFIVDRVKEIIKFKGFQVPPAELESLLVSHPSIADAAVVPQKDEVAGEVPVAF 513

Query: 1536 VVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDLRA 1691
            VV  +G  L+EEA+K+ IAKQVVFYKKLHKV+FV +IPKSP+GKILRKDLRA
Sbjct: 514  VVRSNGFELTEEAVKEFIAKQVVFYKKLHKVHFVHAIPKSPAGKILRKDLRA 565


>dbj|GAV76541.1| AMP-binding domain-containing protein/DUF4009 domain-containing
            protein, partial [Cephalotus follicularis]
          Length = 627

 Score =  777 bits (2006), Expect = 0.0
 Identities = 400/544 (73%), Positives = 450/544 (82%), Gaps = 3/544 (0%)
 Frame = +3

Query: 69   NVEKDDSSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQ 248
            N+   + SS+  +FRSKL D+  I NH+PLHTYCFEN+S FS RPCL++          +
Sbjct: 78   NITSPNPSSETHIFRSKLEDIP-ISNHLPLHTYCFENLSDFSERPCLIVGSTGKVYSFAE 136

Query: 249  THLLSRKIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIF 428
            THL+ +K AAGLS LG+ KGDVIM+LL NCAEF  +FMAASMIGAV TTANPFYTS EIF
Sbjct: 137  THLICQKTAAGLSNLGIKKGDVIMILLHNCAEFVFSFMAASMIGAVSTTANPFYTSTEIF 196

Query: 429  KQFKSSNSKIIITQSQYVNKLVIRESNGEEEYIGQ---VITIDDPPKKCLHFSFLLQNNE 599
            KQF S+ +K+IITQSQYV+KL  R++N     IG+   VITIDDPP+ CLHF+ L + NE
Sbjct: 197  KQFNSARAKLIITQSQYVDKL--RDTNENTPKIGEDFKVITIDDPPENCLHFTVLSEANE 254

Query: 600  ENIPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVL 779
             + P   IDP+D VALPFSSGTTGLPKGVILT KSLITSVAQQVDGENPNLYL ++DVVL
Sbjct: 255  NDTPTATIDPDDPVALPFSSGTTGLPKGVILTQKSLITSVAQQVDGENPNLYLTQEDVVL 314

Query: 780  CVLPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXK 959
            CVLPLFHIYSLNSVLLCSLR+GAAV+LM KFEI +LLELIQ ++V+             K
Sbjct: 315  CVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGALLELIQTHKVSVAAVVPPLVLALAK 374

Query: 960  NPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAK 1139
            NPMV  +DLSSIRVVLSGAAPLGKELE+ LRSR+PQAIFGQGYGMTEAGPVLSM  AFAK
Sbjct: 375  NPMVAKFDLSSIRVVLSGAAPLGKELEEALRSRIPQAIFGQGYGMTEAGPVLSMCLAFAK 434

Query: 1140 NPLATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATI 1319
             P  TKSGSCG VVRNAELKVIDP+T  SLGYNQPGEICIRG QIMKGYLNDAE+TAATI
Sbjct: 435  QPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGQQIMKGYLNDAEATAATI 494

Query: 1320 DEEGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQ 1499
            D EGWLHTGDIGYVD DDEVFIVDRVKE+IKFKGFQVPPAELEALLVSHP IADAAVV Q
Sbjct: 495  DIEGWLHTGDIGYVDDDDEVFIVDRVKEIIKFKGFQVPPAELEALLVSHPSIADAAVVSQ 554

Query: 1500 KDDAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRK 1679
            KD+ AGEVPVAFVV  +G  L+E A+K++IAKQVVFYK+LHKV+FV +IPKSPSGKILRK
Sbjct: 555  KDEVAGEVPVAFVVRSNGFELTEVAVKEYIAKQVVFYKRLHKVHFVHAIPKSPSGKILRK 614

Query: 1680 DLRA 1691
            DLRA
Sbjct: 615  DLRA 618


>ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase 2-like
            [Fragaria vesca subsp. vesca]
          Length = 586

 Score =  775 bits (2001), Expect = 0.0
 Identities = 399/535 (74%), Positives = 439/535 (82%), Gaps = 6/535 (1%)
 Frame = +3

Query: 105  VFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSRKIAAGL 284
            VF+SKLPDL  I NH+PLHTYCFENVS FS RPCL++          +THL+S+K  AGL
Sbjct: 45   VFKSKLPDLPNISNHLPLHTYCFENVSDFSERPCLIVGSTGKTYSFSETHLISQKTGAGL 104

Query: 285  STLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSSNSKIII 464
            S LG++KGDVIM+LLQNCAEF   FM ASMIGAV TTANPFYT+ EIFKQFK+SN+K+II
Sbjct: 105  SKLGITKGDVIMILLQNCAEFVFAFMGASMIGAVTTTANPFYTASEIFKQFKASNAKLII 164

Query: 465  TQSQYVNKLVIRESNGEEEYIG------QVITIDDPPKKCLHFSFLLQNNEENIPPVIID 626
            TQS YV+KL  R S  + +         QV+TIDDPP+ CLHFS L   NE  +P V ID
Sbjct: 165  TQSIYVDKL--RHSGPDSDQYPKLGEHFQVVTIDDPPENCLHFSVLSGANESELPEVSID 222

Query: 627  PNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHIY 806
            P D VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHIY
Sbjct: 223  PEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHIY 282

Query: 807  SLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNPMVGSYDL 986
            SLNSVLLCSLR+GAAV++M KFEI +LLELIQ+YRV              +  MV  YDL
Sbjct: 283  SLNSVLLCSLRAGAAVLVMHKFEIGTLLELIQRYRVXCGSCGATAGDSAGEESMVADYDL 342

Query: 987  SSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNPLATKSGS 1166
            SSIRVVLSGAAPLGKELE+ LRSRVPQA+ GQGYGMTEAGPVLSM  AFAK P  TKSGS
Sbjct: 343  SSIRVVLSGAAPLGKELEEALRSRVPQAVLGQGYGMTEAGPVLSMCLAFAKQPFPTKSGS 402

Query: 1167 CGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDEEGWLHTG 1346
            CG VVRNAELKVI+P+T  SLGYNQPGEICIRG QIMKGYLND  +TA T+D EGWLHTG
Sbjct: 403  CGTVVRNAELKVIEPETGRSLGYNQPGEICIRGSQIMKGYLNDDAATATTVDVEGWLHTG 462

Query: 1347 DIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKDDAAGEVP 1526
            D+GYVD D EVFIVDRVKELIKFKGFQVPPAELE+LLVSH  IADAAVVPQKDDAAGEVP
Sbjct: 463  DVGYVDDDGEVFIVDRVKELIKFKGFQVPPAELESLLVSHQSIADAAVVPQKDDAAGEVP 522

Query: 1527 VAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDLRA 1691
            VAFVV  +G  L+EEA+K+ IAKQVVFYK+LHKVYFV +IPKSPSGKILRKDLRA
Sbjct: 523  VAFVVRSNGNELTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRA 577


>gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]
          Length = 569

 Score =  774 bits (1999), Expect = 0.0
 Identities = 397/544 (72%), Positives = 445/544 (81%), Gaps = 3/544 (0%)
 Frame = +3

Query: 69   NVEKDDSSSKNVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQ 248
            N     S   + +FRSKLPD++ I NH+PLH YCFEN+S FS+RPCL+           +
Sbjct: 18   NQNAPSSHETDHIFRSKLPDIT-ISNHLPLHAYCFENLSDFSDRPCLISGSTGKTYSFAE 76

Query: 249  THLLSRKIAAGLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIF 428
            THL+SRK+AAGLS LG+ KGDVIM LLQNC EF  +FM ASMIGAV TTANPFYT  EIF
Sbjct: 77   THLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMIGAVTTTANPFYTQSEIF 136

Query: 429  KQFKSSNSKIIITQSQYVNKLVIRESNGEEEYIGQ---VITIDDPPKKCLHFSFLLQNNE 599
            KQF +S +K+IITQSQYVNKL   + +   +  G+   VITIDDPP+ C HF+ L++ NE
Sbjct: 137  KQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITIDDPPENCRHFNVLVEANE 196

Query: 600  ENIPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVL 779
              +P + I P+D VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGE PNLYLK+DDVVL
Sbjct: 197  SEMPTISILPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGEIPNLYLKQDDVVL 256

Query: 780  CVLPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXK 959
            CVLPLFHI+SLNSVLLCSLR+G+AV+LM KFEI SLLELIQK+ V+             K
Sbjct: 257  CVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQKHNVSVAAVVPPLVLALAK 316

Query: 960  NPMVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAK 1139
            NP+V ++DLSSIRVVLSGAAPLGKELED LRSRVPQAI GQGYGMTEAGPVLSM  AF+K
Sbjct: 317  NPLVANFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLAFSK 376

Query: 1140 NPLATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATI 1319
             P  TKSGSCG VVRNAELKVIDP+T  SLGYNQPGEICIRG QIMKGYLNDAE+TA TI
Sbjct: 377  QPFPTKSGSCGTVVRNAELKVIDPETGGSLGYNQPGEICIRGSQIMKGYLNDAEATANTI 436

Query: 1320 DEEGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQ 1499
            D EGWLHTGDIGYVD DDE+FIVDRVKE+IKFKGFQVPPAELEALLV+HP IADAAVVPQ
Sbjct: 437  DVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVVPQ 496

Query: 1500 KDDAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRK 1679
            KD+ AGEVPVAFVV    + LSEEA+KD+IAKQVVFYKKLHKV+FV SIPKS SGKILRK
Sbjct: 497  KDEVAGEVPVAFVVRSDDLDLSEEAVKDYIAKQVVFYKKLHKVFFVHSIPKSASGKILRK 556

Query: 1680 DLRA 1691
            DLRA
Sbjct: 557  DLRA 560


>ref|XP_008376904.1| PREDICTED: 4-coumarate--CoA ligase 2 [Malus domestica]
          Length = 605

 Score =  775 bits (2002), Expect = 0.0
 Identities = 393/542 (72%), Positives = 448/542 (82%), Gaps = 11/542 (2%)
 Frame = +3

Query: 99   NVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSRKIAA 278
            N VFRSKLPD+  IPNH+PLHTYCF+N+  FS+RPCL++          +THL+S+K  A
Sbjct: 57   NHVFRSKLPDIP-IPNHLPLHTYCFQNLPEFSDRPCLIVGSTGKSYSFSETHLISQKTGA 115

Query: 279  GLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSSNSKI 458
            GLS LG+ KGDVIM+LLQNCAEF   FM AS+IGAV TTANPFYTS EIFKQ K++N+K+
Sbjct: 116  GLSNLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTSAEIFKQVKAANAKL 175

Query: 459  IITQSQYVNKLVIRESNGE-----------EEYIGQVITIDDPPKKCLHFSFLLQNNEEN 605
            IITQSQYV+KL    S+ +           E++  +V+TIDDPP+ CLHFS L + NE  
Sbjct: 176  IITQSQYVDKLREHPSSADGADQNNYPKLGEDF--KVVTIDDPPENCLHFSVLSEANENE 233

Query: 606  IPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCV 785
            +P V+ID  D VALPFSSGTTGLPKGVILTHKSL+TSVAQQVDGENPNLYLKEDDVVLCV
Sbjct: 234  LPDVVIDAEDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKEDDVVLCV 293

Query: 786  LPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNP 965
            LPLFHI+SLNSVLLCSLR+GA V+LM KFEI +LLELIQ+YRV+             KNP
Sbjct: 294  LPLFHIFSLNSVLLCSLRAGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNP 353

Query: 966  MVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNP 1145
            MV  +DLSSIRVVLSGAAPLGKELE+ L+SRVPQA+ GQGYGMTEAGPVLSM  AFAK P
Sbjct: 354  MVAEFDLSSIRVVLSGAAPLGKELEEALKSRVPQALLGQGYGMTEAGPVLSMCMAFAKEP 413

Query: 1146 LATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDE 1325
            + +KSGSCG VVRNAEL+V+DP+T  SLGYNQPGEICIRG QIMKGYLNDA +TA TID 
Sbjct: 414  MPSKSGSCGTVVRNAELRVLDPETGLSLGYNQPGEICIRGSQIMKGYLNDAAATATTIDT 473

Query: 1326 EGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKD 1505
            EGWLHTGD+GYVD DDEVFIVDRVKELIKFKGFQVPPAELE+LL+SHP IADAAVVPQKD
Sbjct: 474  EGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELESLLISHPSIADAAVVPQKD 533

Query: 1506 DAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDL 1685
            DAAGEVPVAFVV   G+ L+EEA+K+ IAKQVVFYK+LHKV+FV +IPKS SGKILRKDL
Sbjct: 534  DAAGEVPVAFVVRSDGLELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDL 593

Query: 1686 RA 1691
            RA
Sbjct: 594  RA 595


>gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]
          Length = 605

 Score =  775 bits (2002), Expect = 0.0
 Identities = 392/542 (72%), Positives = 449/542 (82%), Gaps = 11/542 (2%)
 Frame = +3

Query: 99   NVVFRSKLPDLSYIPNHVPLHTYCFENVSRFSNRPCLVMXXXXXXXXXXQTHLLSRKIAA 278
            N VFRSKLPD++ IPNH+PLHTYCF+N+  FS+RPCL++          +THL+S+K  A
Sbjct: 57   NHVFRSKLPDIA-IPNHLPLHTYCFQNLPEFSDRPCLIVGSTGKSYSFSETHLISQKTGA 115

Query: 279  GLSTLGVSKGDVIMLLLQNCAEFALTFMAASMIGAVITTANPFYTSGEIFKQFKSSNSKI 458
            GLS LG+ KGDVIM+LLQNCAEF   FM AS+IGAV TTANPFYT+ EIFKQ K++N+K+
Sbjct: 116  GLSNLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTTAEIFKQVKAANAKL 175

Query: 459  IITQSQYVNKLVIRESNGE-----------EEYIGQVITIDDPPKKCLHFSFLLQNNEEN 605
            IITQSQYVNKL    S+ +           E++  +V+TIDDPP+ CLHFS L + NE+ 
Sbjct: 176  IITQSQYVNKLREHPSSADGADQNNYPKLGEDF--KVVTIDDPPENCLHFSVLSEANEKE 233

Query: 606  IPPVIIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCV 785
            +P V+ID  D VALPFSSGTTGLPKGVILTHK+L+TSVAQQVDGENPNLYLKEDDVVLCV
Sbjct: 234  LPDVVIDAEDPVALPFSSGTTGLPKGVILTHKNLVTSVAQQVDGENPNLYLKEDDVVLCV 293

Query: 786  LPLFHIYSLNSVLLCSLRSGAAVVLMPKFEIRSLLELIQKYRVTXXXXXXXXXXXXXKNP 965
            LPLFHI+S NSVLLCSLR+GA V+LM KFEI +LLELIQ+YRV+             KNP
Sbjct: 294  LPLFHIFSFNSVLLCSLRAGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNP 353

Query: 966  MVGSYDLSSIRVVLSGAAPLGKELEDGLRSRVPQAIFGQGYGMTEAGPVLSMSAAFAKNP 1145
            MV  +DLSSIRVVLSGAAPLGKELE+ L+SRVPQA+ GQGYGMTEAGPVLSM  AFAK P
Sbjct: 354  MVAEFDLSSIRVVLSGAAPLGKELEEALKSRVPQAVLGQGYGMTEAGPVLSMCMAFAKEP 413

Query: 1146 LATKSGSCGNVVRNAELKVIDPDTHSSLGYNQPGEICIRGPQIMKGYLNDAESTAATIDE 1325
            + TKSGSCG VVRNAELKV+DP+T  SLGYNQPGEICIRG QIMKGYLNDA +TA TID 
Sbjct: 414  MPTKSGSCGTVVRNAELKVLDPETGLSLGYNQPGEICIRGFQIMKGYLNDAAATATTIDT 473

Query: 1326 EGWLHTGDIGYVDHDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPCIADAAVVPQKD 1505
            EGWLHTGD+GYVD DDEVFIVDRVKELIKFKGFQVPPAELE+LL+SHP IADAAVVPQ+D
Sbjct: 474  EGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELESLLISHPSIADAAVVPQRD 533

Query: 1506 DAAGEVPVAFVVLLSGMALSEEAIKDHIAKQVVFYKKLHKVYFVTSIPKSPSGKILRKDL 1685
            DAAGEVPVAFVV   G+ L+EEA+K+ IAKQVVFYK+LHKV+FV +IPKS SGKILRKDL
Sbjct: 534  DAAGEVPVAFVVRSDGLELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDL 593

Query: 1686 RA 1691
            RA
Sbjct: 594  RA 595