BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00046274
         (765 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|OTF93917.1| putative debranching enzyme 1 [Helianthus annuus]      254   1e-75
ref|XP_022010633.1| isoamylase 2, chloroplastic [Helianthus annu...   240   2e-70
gb|KVH94413.1| Glycoside hydrolase, catalytic domain-containing ...   231   7e-67
ref|XP_023738402.1| isoamylase 2, chloroplastic isoform X1 [Lact...   231   8e-67
ref|XP_023738403.1| isoamylase 2, chloroplastic isoform X2 [Lact...   206   8e-58
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                             130   1e-30
ref|XP_018858427.1| PREDICTED: isoamylase 2, chloroplastic [Jugl...   114   3e-25
ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nico...   114   4e-25
ref|XP_021613460.1| isoamylase 2, chloroplastic [Manihot esculen...   112   3e-24
ref|XP_017978673.1| PREDICTED: isoamylase 2, chloroplastic [Theo...   110   1e-23
gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]                 109   2e-23
gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]        109   2e-23
ref|XP_009356744.1| PREDICTED: isoamylase 2, chloroplastic-like ...   108   6e-23
gb|OMO77794.1| hypothetical protein CCACVL1_14832 [Corchorus cap...   108   6e-23
ref|XP_008386532.1| PREDICTED: isoamylase 2, chloroplastic-like ...   107   1e-22
ref|XP_021681351.1| isoamylase 2, chloroplastic isoform X2 [Heve...   105   3e-22
gb|OMP04997.1| hypothetical protein COLO4_09141 [Corchorus olito...   105   4e-22
ref|XP_021681350.1| isoamylase 2, chloroplastic isoform X1 [Heve...   105   4e-22
ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nico...   105   5e-22
ref|XP_008368864.1| PREDICTED: isoamylase 2, chloroplastic-like ...   105   5e-22

>gb|OTF93917.1| putative debranching enzyme 1 [Helianthus annuus]
          Length = 847

 Score =  254 bits (650), Expect = 1e-75
 Identities = 132/208 (63%), Positives = 156/208 (75%), Gaps = 3/208 (1%)
 Frame = -3

Query: 616 FWFLQRICLHSWLDITMAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKN 437
           FWFLQ ICL S L   M+ML+ SSATQP CLTCGTIK+S LI +D+CKYG R TQ+FG+ 
Sbjct: 4   FWFLQVICLQSAL---MSMLSLSSATQPTCLTCGTIKISKLIIADSCKYGRRATQSFGRM 60

Query: 436 NLGRNLVLNEVA---IKGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVIT 266
           +LG+NLV  + A   IKG Y   R  +LA ST+PV QT +KLSTY FRTEKGGNV V+IT
Sbjct: 61  DLGQNLVFGKTAKNIIKGLYHNPRFGILAASTLPVIQTAEKLSTYLFRTEKGGNVTVIIT 120

Query: 265 KLKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGIFETPFVNNLLN 86
           +LK+GKYGVN+E SSLQL+GND D++MSWG+FR             SGIFETPF  + LN
Sbjct: 121 RLKDGKYGVNVEISSLQLTGNDADIIMSWGVFRSDSSSRIPIHSSDSGIFETPFAIDSLN 180

Query: 85  KLSVELEFDASLAPFYVSFLLKSSLNGE 2
           K S ELEFDASLAPFY+SFLLK  L+GE
Sbjct: 181 KFSAELEFDASLAPFYISFLLKWHLDGE 208


>ref|XP_022010633.1| isoamylase 2, chloroplastic [Helianthus annuus]
 ref|XP_022010634.1| isoamylase 2, chloroplastic [Helianthus annuus]
          Length = 831

 Score =  240 bits (613), Expect = 2e-70
 Identities = 122/192 (63%), Positives = 146/192 (76%), Gaps = 3/192 (1%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---I 398
           M+ML+ SSATQP CLTCGTIK+S LI +D+CKYG R TQ+FG+ +LG+NLV  + A   I
Sbjct: 1   MSMLSLSSATQPTCLTCGTIKISKLIIADSCKYGRRATQSFGRMDLGQNLVFGKTAKNII 60

Query: 397 KGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSL 218
           KG Y   R  +LA ST+PV QT +KLSTY FRTEKGGNV V+IT+LK+GKYGVN+E SSL
Sbjct: 61  KGLYHNPRFGILAASTLPVIQTAEKLSTYLFRTEKGGNVTVIITRLKDGKYGVNVEISSL 120

Query: 217 QLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGIFETPFVNNLLNKLSVELEFDASLAPFY 38
           QL+GND D++MSWG+FR             SGIFETPF  + LNK S ELEFDASLAPFY
Sbjct: 121 QLTGNDADIIMSWGVFRSDSSSRIPIHSSDSGIFETPFAIDSLNKFSAELEFDASLAPFY 180

Query: 37  VSFLLKSSLNGE 2
           +SFLLK  L+GE
Sbjct: 181 ISFLLKWHLDGE 192


>gb|KVH94413.1| Glycoside hydrolase, catalytic domain-containing protein [Cynara
           cardunculus var. scolymus]
          Length = 841

 Score =  231 bits (589), Expect = 7e-67
 Identities = 126/192 (65%), Positives = 146/192 (76%), Gaps = 3/192 (1%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---I 398
           MAML+ SSAT P  LTCGT KLS LI +D+CKYG RVT++ G+ ++ R+LV  EVA   I
Sbjct: 1   MAMLSLSSATHPRYLTCGTTKLSKLIIADSCKYGRRVTRSLGRMDMERSLVFGEVAKNMI 60

Query: 397 KGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSL 218
           KGS    R+ VLA ST+PV +TV+KLSTY FRTEKGG+VKV IT+LK+ KYGV++E S L
Sbjct: 61  KGSCHNLRVGVLAASTIPVIETVEKLSTYLFRTEKGGHVKV-ITRLKDDKYGVDVEVSYL 119

Query: 217 QLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGIFETPFVNNLLNKLSVELEFDASLAPFY 38
           QLSGND DVVMSWGIFR             SGI ETPFVN  LNKLSVELEFDASLAPFY
Sbjct: 120 QLSGNDGDVVMSWGIFRSDSSSLMSLRSNDSGILETPFVNKSLNKLSVELEFDASLAPFY 179

Query: 37  VSFLLKSSLNGE 2
           +SFLLKS L+GE
Sbjct: 180 ISFLLKSQLSGE 191


>ref|XP_023738402.1| isoamylase 2, chloroplastic isoform X1 [Lactuca sativa]
          Length = 876

 Score =  231 bits (590), Expect = 8e-67
 Identities = 132/213 (61%), Positives = 148/213 (69%), Gaps = 7/213 (3%)
 Frame = -3

Query: 619 IFWFLQRICLHSWL----DITMAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQ 452
           I WFLQ +CLHSW+    DI+ AM++ SSATQP  LTCGTIK+S LI +D+CKYG RV Q
Sbjct: 27  IIWFLQVLCLHSWVVLHSDISTAMISLSSATQPSYLTCGTIKVSKLIIADSCKYGRRVAQ 86

Query: 451 NFGKNNLGRNLVLNEVA---IKGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNV 281
             G+ N+ +NLV   VA   IK S   RRI VLA ST PV Q   KLSTY FRTEKGG+V
Sbjct: 87  TPGRINVEQNLVFGLVAKNMIKDSCHARRISVLAASTFPVIQPANKLSTYLFRTEKGGHV 146

Query: 280 KVVITKLKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGIFETPFV 101
           KV IT+LKN KY VN+E S L    NDDDV+MSWGIFR             SGI ETPFV
Sbjct: 147 KV-ITELKNDKYRVNLEVSPLH--DNDDDVIMSWGIFRSDSSSLIPLESSDSGISETPFV 203

Query: 100 NNLLNKLSVELEFDASLAPFYVSFLLKSSLNGE 2
           N  LNKLS ELEFDASLAPF+VSFLLKS   GE
Sbjct: 204 NKSLNKLSTELEFDASLAPFHVSFLLKSHFGGE 236


>ref|XP_023738403.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
 ref|XP_023738404.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
 ref|XP_023738405.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
 ref|XP_023738406.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
 gb|PLY70241.1| hypothetical protein LSAT_9X4440 [Lactuca sativa]
          Length = 827

 Score =  206 bits (525), Expect = 8e-58
 Identities = 119/190 (62%), Positives = 132/190 (69%), Gaps = 3/190 (1%)
 Frame = -3

Query: 562 MLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---IKG 392
           M++ SSATQP  LTCGTIK+S LI +D+CKYG RV Q  G+ N+ +NLV   VA   IK 
Sbjct: 1   MISLSSATQPSYLTCGTIKVSKLIIADSCKYGRRVAQTPGRINVEQNLVFGLVAKNMIKD 60

Query: 391 SYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSLQL 212
           S   RRI VLA ST PV Q   KLSTY FRTEKGG+VKV IT+LKN KY VN+E S L  
Sbjct: 61  SCHARRISVLAASTFPVIQPANKLSTYLFRTEKGGHVKV-ITELKNDKYRVNLEVSPLH- 118

Query: 211 SGNDDDVVMSWGIFRXXXXXXXXXXXXXSGIFETPFVNNLLNKLSVELEFDASLAPFYVS 32
             NDDDV+MSWGIFR             SGI ETPFVN  LNKLS ELEFDASLAPF+VS
Sbjct: 119 -DNDDDVIMSWGIFRSDSSSLIPLESSDSGISETPFVNKSLNKLSTELEFDASLAPFHVS 177

Query: 31  FLLKSSLNGE 2
           FLLKS   GE
Sbjct: 178 FLLKSHFGGE 187


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score =  130 bits (327), Expect = 1e-30
 Identities = 84/198 (42%), Positives = 110/198 (55%), Gaps = 9/198 (4%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEV---AI 398
           MA L  S   Q  C+ CG  + S  + +    Y  RV QN  + +  RN V  EV   A 
Sbjct: 1   MATLPLSFTIQSHCVYCGATESSKSVVATHFNYRKRVAQNL-QQSYKRNFVHGEVKKYAT 59

Query: 397 KGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSL 218
           K S++ R ++VLATS + V Q  +K S Y FRTE GG+VKV++ + KN KY V +E SSL
Sbjct: 60  KDSFQHRNLKVLATSRISVGQKEEKSSAYLFRTEIGGHVKVLVRQ-KNLKYSVYVEVSSL 118

Query: 217 QLSGNDDDVVMSWGIFRXXXXXXXXXXXXXS------GIFETPFVNNLLNKLSVELEFDA 56
           +L   +D +VM+WGIFR             S         ETPFV N L KL +EL F+ 
Sbjct: 119 KLKSGEDKLVMNWGIFRSDSSQFIPLDLQSSTPDDRSSTVETPFVQNSLGKLVIELNFEG 178

Query: 55  SLAPFYVSFLLKSSLNGE 2
           SL PFY+SFLLK   +G+
Sbjct: 179 SLTPFYLSFLLKFPSDGD 196


>ref|XP_018858427.1| PREDICTED: isoamylase 2, chloroplastic [Juglans regia]
          Length = 889

 Score =  114 bits (286), Expect = 3e-25
 Identities = 78/207 (37%), Positives = 108/207 (52%), Gaps = 18/207 (8%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAIKGS 389
           MA L+ S A +P CLTCG  + S    +    Y  ++       +L R L+  EVA K S
Sbjct: 1   MATLSPSLAIRPCCLTCGASQSSKSSLAYHYIYRNQIRCGIENTDLERKLICGEVA-KTS 59

Query: 388 YRTRRIQVLATSTVPVTQTVKKLST------------YSFRTEKGGNVKVVITKLKNGKY 245
           YR    +V ATS V + +T ++++T            Y F TE GG VKV + K KN KY
Sbjct: 60  YRHLNSKVYATSRVFIKETEQRVTTITEVEDMLKSLTYLFWTEIGGQVKVSVRK-KNVKY 118

Query: 244 GVNIEASSLQLSGNDDDVVMSWGIFR------XXXXXXXXXXXXXSGIFETPFVNNLLNK 83
            V +E SSLQL G+DD +++SWGI+R                   +   ETPF+ N   +
Sbjct: 119 AVYVEVSSLQLHGSDDRLLLSWGIYRDDSSCFMHLDAQSSTPDGRATTRETPFIQNTKGR 178

Query: 82  LSVELEFDASLAPFYVSFLLKSSLNGE 2
             +EL+F+    PFY+SFLLKSSL  +
Sbjct: 179 FVLELDFEEKKIPFYLSFLLKSSLGSD 205


>ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana attenuata]
 gb|OIT28559.1| isoamylase 2, chloroplastic [Nicotiana attenuata]
          Length = 863

 Score =  114 bits (285), Expect = 4e-25
 Identities = 77/201 (38%), Positives = 104/201 (51%), Gaps = 15/201 (7%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAIKG- 392
           MA LT   A Q   L+ G  + + L+ +    +G RV          R L L E+   G 
Sbjct: 1   MATLTVQLAVQSRLLSYGIGESTKLVPAPPANHGKRVLCRL------RKLELEEINFSGR 54

Query: 391 --------SYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVN 236
                   S RT R++ L+TS + + QT K + TY FRT+ GG VKV++ K  NGKY V 
Sbjct: 55  SRKTDQEVSKRTHRLKALSTSGISLIQTAKTVPTYLFRTDTGGQVKVLVEKT-NGKYKVL 113

Query: 235 IEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGI------FETPFVNNLLNKLSV 74
           +EA   +LS    ++VM WG+FR              G        ETPFV    NK++V
Sbjct: 114 VEALPSELSDAHAELVMVWGLFRSDASCFMPLDLNRRGADGKSSTIETPFVQGPSNKVTV 173

Query: 73  ELEFDASLAPFYVSFLLKSSL 11
           EL+F+ASLAPFY+SF +KS L
Sbjct: 174 ELDFEASLAPFYISFYMKSQL 194


>ref|XP_021613460.1| isoamylase 2, chloroplastic [Manihot esculenta]
 gb|OAY49666.1| hypothetical protein MANES_05G073400 [Manihot esculenta]
 gb|AUZ20773.1| isoamylase 2 (plastid) [Manihot esculenta]
          Length = 883

 Score =  112 bits (279), Expect = 3e-24
 Identities = 74/206 (35%), Positives = 104/206 (50%), Gaps = 19/206 (9%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAIK-- 395
           MA L  S A    C +CG ++ S L  +     G ++   FG+ ++ + L++ EVA    
Sbjct: 1   MATLLPSFAISRCCYSCGAVESSKLTLTTRYTSGKKMELGFGRTDVEKRLLVGEVAQNVR 60

Query: 394 ---------GSYRTRRIQVLAT----STVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKN 254
                    G +   R+ V  T    ST+     ++K+S+Y FRT+ GGNVKV + K KN
Sbjct: 61  STLHWNHNSGVFAAARVPVQETEQILSTITEVDELQKVSSYLFRTQIGGNVKVSVRK-KN 119

Query: 253 GKYGVNIEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSG----IFETPFVNNLLN 86
            KY V IE SSL+L  +D  +V++WGI+R                     ETPFV N   
Sbjct: 120 AKYAVYIEVSSLELGNSDYRLVLAWGIYRSDSSCFMPLDSQRLDPVARTMETPFVQNAFA 179

Query: 85  KLSVELEFDASLAPFYVSFLLKSSLN 8
             S+ELEF+A   PF +SFLLKS  N
Sbjct: 180 IFSLELEFEAKQTPFSLSFLLKSMFN 205


>ref|XP_017978673.1| PREDICTED: isoamylase 2, chloroplastic [Theobroma cacao]
          Length = 867

 Score =  110 bits (274), Expect = 1e-23
 Identities = 77/198 (38%), Positives = 108/198 (54%), Gaps = 14/198 (7%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---I 398
           MA L  S A  P CL  G  +LS L F       +++ Q+  + ++GR L   EVA   +
Sbjct: 1   MATLPPSLAIGPHCLNSGATELSKLSFVTRYLCKSKMGQSLQRIDVGRKLFSGEVAQNVM 60

Query: 397 KGSYRTRRIQVLATSTVPVTQT-----------VKKLSTYSFRTEKGGNVKVVITKLKNG 251
           +  Y    ++  A S V V QT           +KKLSTY FRTE GG VKV++ K ++ 
Sbjct: 61  QSRYWNLDLRCFAASRVSVEQTEQIFTSTQVDELKKLSTYLFRTEIGGQVKVLVRK-RSV 119

Query: 250 KYGVNIEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGIFETPFVNNLLNKLSVE 71
            + V+IE SSLQLSG++  +V+S G+ R               I ETPF+    ++L++E
Sbjct: 120 NHVVDIEVSSLQLSGDESQLVLSGGVHRSDHDIKNR-------IIETPFIAKSSSELALE 172

Query: 70  LEFDASLAPFYVSFLLKS 17
           LEF+A  APFY SFLLK+
Sbjct: 173 LEFEAKEAPFYFSFLLKA 190


>gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score =  109 bits (273), Expect = 2e-23
 Identities = 78/198 (39%), Positives = 107/198 (54%), Gaps = 14/198 (7%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---I 398
           MA L  S A  P CL  G  +LS L F       +++ Q+  + ++GR L   EVA   +
Sbjct: 1   MATLPPSLAIGPHCLNSGATELSKLSFVTRYLCKSKMGQSLQRIDVGRKLFSGEVAQNVM 60

Query: 397 KGSYRTRRIQVLATSTVPVTQT-----------VKKLSTYSFRTEKGGNVKVVITKLKNG 251
           +  Y    ++  A S V V QT           +KKLSTY FRTE GG VKV + K ++ 
Sbjct: 61  QSRYWNLDLRCFAASRVSVEQTEQIFTSTQVDELKKLSTYLFRTEIGGQVKVFVRK-RSV 119

Query: 250 KYGVNIEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGIFETPFVNNLLNKLSVE 71
            + V+IE SSLQLSG++  +V+S GI R               I ETPF+    ++L++E
Sbjct: 120 NHVVDIEVSSLQLSGDESQLVLSGGIHRSDHDIKNR-------IIETPFIAKSSSELALE 172

Query: 70  LEFDASLAPFYVSFLLKS 17
           LEF+A  APFY SFLLK+
Sbjct: 173 LEFEAKEAPFYFSFLLKA 190


>gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]
          Length = 844

 Score =  109 bits (272), Expect = 2e-23
 Identities = 72/181 (39%), Positives = 104/181 (57%), Gaps = 9/181 (4%)
 Frame = -3

Query: 529 CLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRN---LVLNEVAIKGSYRTRRIQVLA 359
           C +CG  + S L  +   KY   VT++  K +L +    + LN+   + S     ++ LA
Sbjct: 8   CPSCGASESSYLFAAKFGKYVEWVTRDLVKFDLKQKKDRVRLNKFGGRISLEHHVMKALA 67

Query: 358 TSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEASSLQLSGNDDDVVMSW 179
           TS V V QT  KL++Y FRTE GG +KV++ K  N K+ V++EAS+L     D+ ++M W
Sbjct: 68  TSDVSVVQTTGKLASYRFRTEIGGQLKVLVRK--NSKFEVHVEASNLP-GEEDEGLIMRW 124

Query: 178 GIFRXXXXXXXXXXXXXS------GIFETPFVNNLLNKLSVELEFDASLAPFYVSFLLKS 17
           G+FR             S       + ETPF+ + + +L+VEL+FDASL PFYVSFLLKS
Sbjct: 125 GLFRSDSSIFMPLNFQQSTADSKDNVVETPFLKDSVGRLAVELDFDASLTPFYVSFLLKS 184

Query: 16  S 14
           S
Sbjct: 185 S 185


>ref|XP_009356744.1| PREDICTED: isoamylase 2, chloroplastic-like [Pyrus x
           bretschneideri]
          Length = 870

 Score =  108 bits (269), Expect = 6e-23
 Identities = 83/214 (38%), Positives = 109/214 (50%), Gaps = 25/214 (11%)
 Frame = -3

Query: 568 MAMLTSSSATQPP-CLTCGTIKLSTLIFSDTCKY---GTRVTQNFGKNNLGRNLVLNEVA 401
           MA L  S A Q   CL CGT +LS L  ++   +   G RV+   G   + RNLV  EV 
Sbjct: 1   MATLPLSIAIQASFCLNCGTTELSKLTAANRSIHRHNGLRVSVKLG---IERNLVFGEVL 57

Query: 400 I---KGSYRTRRIQVLATSTVPV------------TQTVKKLSTYSFRTEKGGNVKVVIT 266
               +   R R ++V ATS V V            T+   K+STY FRTE G  VKV + 
Sbjct: 58  QNFKETPLRDRDLKVYATSRVSVEPMEQKVYTSTETEEAGKVSTYRFRTETGDLVKVFV- 116

Query: 265 KLKNGKYGVNIEASSLQLSGNDDDVVMSWGIFR------XXXXXXXXXXXXXSGIFETPF 104
           ++KN KY VNIE SSL LS ND  +V+SWGI+R                   +   ETPF
Sbjct: 117 RMKNAKYIVNIEVSSLHLSSNDRLLVLSWGIYRSDASSFMPSNFRSSTPADRTTTLETPF 176

Query: 103 VNNLLNKLSVELEFDASLAPFYVSFLLKSSLNGE 2
                 + ++ELEF+A   PFY+SF+LKS  + +
Sbjct: 177 TETSSGRFTLELEFEAKQIPFYLSFILKSPADAD 210


>gb|OMO77794.1| hypothetical protein CCACVL1_14832 [Corchorus capsularis]
          Length = 878

 Score =  108 bits (269), Expect = 6e-23
 Identities = 77/200 (38%), Positives = 108/200 (54%), Gaps = 15/200 (7%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEV---AI 398
           MA L  S    P CL CG    S L         ++V Q+  + ++GR L   EV   A+
Sbjct: 1   MATLPPSLTIGPCCLKCGAPDSSRLSVVARYLCKSKVGQSLQRIDVGRKLFPGEVVQSAL 60

Query: 397 KGSYRTRRIQVLATSTVPVTQT------------VKKLSTYSFRTEKGGNVKVVITKLKN 254
           +  + +  ++  A S V V QT            +KKLSTY FRTE GG VK+ + K K+
Sbjct: 61  QPPHFSLDLRCFAASRVSVEQTEQTFTSTSQVDELKKLSTYMFRTEIGGQVKIFVRK-KS 119

Query: 253 GKYGVNIEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGIFETPFVNNLLNKLSV 74
            KY V+IE SSLQLSG+++ +V+S G++R                 ETPF+    ++L++
Sbjct: 120 VKYVVDIEVSSLQLSGDNNKLVLSGGVYRSDHDVKTNN-------IETPFIARSSSELAL 172

Query: 73  ELEFDASLAPFYVSFLLKSS 14
           ELEF+A  APFY SFLLK+S
Sbjct: 173 ELEFEAKEAPFYFSFLLKAS 192


>ref|XP_008386532.1| PREDICTED: isoamylase 2, chloroplastic-like [Malus domestica]
          Length = 870

 Score =  107 bits (266), Expect = 1e-22
 Identities = 79/211 (37%), Positives = 106/211 (50%), Gaps = 22/211 (10%)
 Frame = -3

Query: 568 MAMLTSSSATQPPC-LTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVAI-- 398
           MA L  S A Q  C L CGT +LS L  ++  ++     +   K  + RNLV  EV    
Sbjct: 1   MATLPLSIAMQASCCLNCGTTELSKLTAANRYRHRHNGLRGSVKLGIERNLVFGEVVQNF 60

Query: 397 -KGSYRTRRIQVLATSTVPV------------TQTVKKLSTYSFRTEKGGNVKVVITKLK 257
            +   R R ++V ATS V V            T+   K+STY FRTE G  VKV + ++K
Sbjct: 61  KETPLRDRDLKVYATSRVSVEPMEQRVYTSTETEEAGKVSTYRFRTETGDMVKVFV-RMK 119

Query: 256 NGKYGVNIEASSLQLSGNDDDVVMSWGIFR------XXXXXXXXXXXXXSGIFETPFVNN 95
           N K  VNIE SSL LS ND  +V+SWGI+R                   +   ETPF   
Sbjct: 120 NAKCIVNIEVSSLHLSSNDRLLVLSWGIYRSDSSSFMPSNFRSSTPADRTTTLETPFTET 179

Query: 94  LLNKLSVELEFDASLAPFYVSFLLKSSLNGE 2
              + ++ELEF+A   PFY+SF+LKS  + +
Sbjct: 180 CSGRFTLELEFEAKQIPFYLSFILKSPADAD 210


>ref|XP_021681351.1| isoamylase 2, chloroplastic isoform X2 [Hevea brasiliensis]
          Length = 842

 Score =  105 bits (263), Expect = 3e-22
 Identities = 73/209 (34%), Positives = 104/209 (49%), Gaps = 22/209 (10%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---- 401
           MA L  S A  P   +C  ++ S L  +     G ++    G  ++ + L++ EVA    
Sbjct: 1   MATLPLSLAKSPCFYSCRAVESSKLTVTAHYISGKKLALGLGGMDVEKGLLVGEVAQNVR 60

Query: 400 --IKGSYRTRRIQVLATSTVPVTQT------------VKKLSTYSFRTEKGGNVKVVITK 263
             + G+  +R     A + VPV QT            +KK+STY FRT+ GGNVKV + K
Sbjct: 61  KALHGNLSSREF---AAARVPVQQTEQIFSTTSEVDELKKVSTYLFRTQNGGNVKVFVRK 117

Query: 262 LKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSG----IFETPFVNN 95
            KN KY + IE SSL+L  +D  +V++WGI+R                     +TPFV N
Sbjct: 118 -KNAKYALYIEVSSLELGTSDYRLVLTWGIYRSDSSCFMPLDSQHLDPGARTMDTPFVQN 176

Query: 94  LLNKLSVELEFDASLAPFYVSFLLKSSLN 8
              + S+ELEF+    PFY+SFLLK   N
Sbjct: 177 TFGRFSLELEFEVKQTPFYLSFLLKCMFN 205


>gb|OMP04997.1| hypothetical protein COLO4_09141 [Corchorus olitorius]
          Length = 868

 Score =  105 bits (263), Expect = 4e-22
 Identities = 76/200 (38%), Positives = 107/200 (53%), Gaps = 15/200 (7%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEV---AI 398
           MA L  S      CL CG    S L         ++V Q+  + ++GR L   EV   A+
Sbjct: 1   MATLPPSLTIGRCCLKCGAPDSSRLSVVARYLCKSKVGQSLQRIDVGRKLFPGEVVQSAL 60

Query: 397 KGSYRTRRIQVLATSTVPVTQT------------VKKLSTYSFRTEKGGNVKVVITKLKN 254
           +  + +  ++  A S V V QT            +KKLSTY FRTE GG VK+ + K K+
Sbjct: 61  QPPHLSLDLRCFAASRVSVEQTEQTFTSTSQVDELKKLSTYMFRTEIGGQVKIFVRK-KS 119

Query: 253 GKYGVNIEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGIFETPFVNNLLNKLSV 74
            KY V+IE SSLQLSGN++ +V+S G++R                 E PF+    ++L++
Sbjct: 120 VKYVVDIEVSSLQLSGNNNKLVLSGGVYRSDHDIKTNN-------IEAPFIARSSSELAL 172

Query: 73  ELEFDASLAPFYVSFLLKSS 14
           ELEF+A  APFY+SFLLK+S
Sbjct: 173 ELEFEAKEAPFYLSFLLKAS 192


>ref|XP_021681350.1| isoamylase 2, chloroplastic isoform X1 [Hevea brasiliensis]
          Length = 883

 Score =  105 bits (263), Expect = 4e-22
 Identities = 73/209 (34%), Positives = 104/209 (49%), Gaps = 22/209 (10%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLGRNLVLNEVA---- 401
           MA L  S A  P   +C  ++ S L  +     G ++    G  ++ + L++ EVA    
Sbjct: 1   MATLPLSLAKSPCFYSCRAVESSKLTVTAHYISGKKLALGLGGMDVEKGLLVGEVAQNVR 60

Query: 400 --IKGSYRTRRIQVLATSTVPVTQT------------VKKLSTYSFRTEKGGNVKVVITK 263
             + G+  +R     A + VPV QT            +KK+STY FRT+ GGNVKV + K
Sbjct: 61  KALHGNLSSREF---AAARVPVQQTEQIFSTTSEVDELKKVSTYLFRTQNGGNVKVFVRK 117

Query: 262 LKNGKYGVNIEASSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSG----IFETPFVNN 95
            KN KY + IE SSL+L  +D  +V++WGI+R                     +TPFV N
Sbjct: 118 -KNAKYALYIEVSSLELGTSDYRLVLTWGIYRSDSSCFMPLDSQHLDPGARTMDTPFVQN 176

Query: 94  LLNKLSVELEFDASLAPFYVSFLLKSSLN 8
              + S+ELEF+    PFY+SFLLK   N
Sbjct: 177 TFGRFSLELEFEVKQTPFYLSFLLKCMFN 205


>ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
 ref|XP_018625653.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
          Length = 863

 Score =  105 bits (262), Expect = 5e-22
 Identities = 72/198 (36%), Positives = 103/198 (52%), Gaps = 12/198 (6%)
 Frame = -3

Query: 568 MAMLTSSSATQPPCLTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNL------GRNLVLNE 407
           MA  T   A Q   L+ G  + + L+ +     G RV     K  L      GR+  +++
Sbjct: 1   MATSTVQLAVQSRLLSYGIGESTELVPAPPANRGKRVVCRLRKLELEEMNFSGRSRKIDQ 60

Query: 406 VAIKGSYRTRRIQVLATSTVPVTQTVKKLSTYSFRTEKGGNVKVVITKLKNGKYGVNIEA 227
              K   RT R++ L+ S + + QT K++ TY FRT+ GG VKV++ K  NGKY V +E 
Sbjct: 61  EVSK---RTHRLKALSASGISLIQTAKRVPTYLFRTDIGGQVKVLVEKT-NGKYKVLVEV 116

Query: 226 SSLQLSGNDDDVVMSWGIFRXXXXXXXXXXXXXSGI------FETPFVNNLLNKLSVELE 65
              +LS    ++VM WG+FR              G        ETPFV    +K++VEL+
Sbjct: 117 LPSELSDAHAELVMVWGLFRSDASCFMPLDLNRHGADGKSSTIETPFVQGPSDKVTVELD 176

Query: 64  FDASLAPFYVSFLLKSSL 11
           F+ASLAPFY+SF +KS L
Sbjct: 177 FEASLAPFYISFYMKSQL 194


>ref|XP_008368864.1| PREDICTED: isoamylase 2, chloroplastic-like [Malus domestica]
          Length = 870

 Score =  105 bits (262), Expect = 5e-22
 Identities = 83/213 (38%), Positives = 106/213 (49%), Gaps = 24/213 (11%)
 Frame = -3

Query: 568 MAMLTSSSATQPPC-LTCGTIKLSTLIFSDTCKYGTRVTQNFGKNNLG--RNLVLNEVAI 398
           MA L  S A Q  C L CGT +L  L  +D  +YG R     G   LG  RNLVL EV  
Sbjct: 1   MATLPLSIAIQASCCLNCGTTELPKLTATD--RYGHRNNGVHGYVKLGTERNLVLREVVQ 58

Query: 397 ---KGSYRTRRIQVLATSTVPV------------TQTVKKLSTYSFRTEKGGNVKVVITK 263
              +   R R  +V ATS V V            T+ V K+STY FRT+ G  V V + K
Sbjct: 59  ILKERPLRDREWKVYATSRVSVEPVEQRVSTGTETEEVGKVSTYLFRTDTGDPVNVFVRK 118

Query: 262 LKNGKYGVNIEASSLQLSGNDDDVVMSWGIFR------XXXXXXXXXXXXXSGIFETPFV 101
            +N KY VNIE S L LS +D  +V+SWG++R                   +   ETPF 
Sbjct: 119 -RNAKYAVNIEVSLLHLSRSDRRLVLSWGMYRADSSSFVPSNFRSSTPADRTTALETPFT 177

Query: 100 NNLLNKLSVELEFDASLAPFYVSFLLKSSLNGE 2
                K ++ELEF+A   PFY+SF+LKS  + +
Sbjct: 178 GTSSGKFTLELEFEAKQIPFYLSFVLKSPADAD 210